CmoCh03G014960 (gene) Cucurbita moschata (Rifu) v1

BLAST of CmoCh03G014960 vs. ExPASy Swiss-Prot
Match: F4JT76 (Vacuolar protein sorting-associated protein 54, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=VPS54 PE=1 SV=1)

HSP 1 Score: 1242.3 bits (3213), Expect = 0.0e+00
Identity = 679/1038 (65.41%), Postives = 818/1038 (78.81%), Query Frame = 0

Query: 58   MESQPSQSGRSPT--DYSSL-ISRETRLDRTISSPSAKSNTDASSQSLSSILNNPHAGK- 117
            M+S PS  GRS T  + SSL + R +    +  SP  KS +DASSQSLSSILNNPH GK 
Sbjct: 1    MDSHPSLMGRSITNSNRSSLDLGRPSSSSSSSPSPLTKSISDASSQSLSSILNNPHGGKS 60

Query: 118  ----SDASWVGWWSSPSTVSPPEFMPLAST-IASSEVTRLDFNSYTAVISDPFNRFEDIR 177
                SDASWVGWWSS + V+P EF P+AST +  SE+TR DF+ Y + IS+   RFEDIR
Sbjct: 61   GVYGSDASWVGWWSSSTFVAPAEFAPVASTKLPGSELTRSDFHGYVSSISESHGRFEDIR 120

Query: 178  NHSSKEDGGLDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVSQNFVLQ 237
             H+ +E  G D        L ACLREVP+LYFKEDFALEDGATFR+ACPF ++++N  LQ
Sbjct: 121  KHTREESCGFDQ-ESHVSGLAACLREVPSLYFKEDFALEDGATFRSACPFSSLNENLALQ 180

Query: 238  EKLSHYLDVVELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQIKETIRLLDVDL 297
            EKLS YLDVVELHLVKEIS+RS+SFFEAQGQLQDLNVKIVEGCSRIR++KETIRLLD +L
Sbjct: 181  EKLSQYLDVVELHLVKEISVRSDSFFEAQGQLQDLNVKIVEGCSRIRELKETIRLLDRNL 240

Query: 298  VDSAREIQAQNATRNNLLALQHKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLL 357
            VDSAR+IQ  ++TR N+L LQ KL+LILYVNQA+SALKLLVASADCAGALD+TDDL +LL
Sbjct: 241  VDSARQIQELSSTRINMLELQRKLRLILYVNQALSALKLLVASADCAGALDITDDLQNLL 300

Query: 358  EGDELAGLHCFRHLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKARASNLM 417
             GDEL GL+CFRHLRDHV +SI+SI SIL++EFMR SIHD G++D++I++    R S   
Sbjct: 301  AGDELTGLYCFRHLRDHVTSSIDSINSILTSEFMRISIHDTGEIDVLILSAANIRGSISS 360

Query: 418  NGK-DEQVKLDEEETSNFCDHLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAFKNAVAE 477
            NG   E+VKL+EE+TS  CD LLP++IGLLRTAK PS+LR+YRD +T++MK A K AVA+
Sbjct: 361  NGNTGEEVKLEEEDTSTLCDRLLPLVIGLLRTAKFPSILRMYRDTLTSEMKNAIKKAVAD 420

Query: 478  LLPVLLLRPLDSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAILKIVQVHLVRA 537
            LLP+L+ R L+SDF+ GER+VD DGGG SLASKLR LSSE FV LL+AI KIVQ HLVRA
Sbjct: 421  LLPILVARSLESDFSHGERSVD-DGGGLSLASKLRTLSSEAFVNLLTAIFKIVQAHLVRA 480

Query: 538  AEVKKSIEWIMCNLDGHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKVT 597
            +EVKK+IEWI+CN+DGHYAADSVAAAI +GA A+ TAQ+   QGG L+     +A +K  
Sbjct: 481  SEVKKAIEWILCNIDGHYAADSVAAAIAVGAVAAETAQEIGFQGGSLVSSPLGKATSKAP 540

Query: 598  SSQGKENEAANPSNMSRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRTLVHPKLRLQE 657
              QGK ++A++  NMSRNFRADVLRENTEAVFAAC+  HGRWAKLLGVR L+HPKL+LQE
Sbjct: 541  PLQGKSSDASSLMNMSRNFRADVLRENTEAVFAACEVTHGRWAKLLGVRALLHPKLKLQE 600

Query: 658  FLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVE 717
            F+SIY++TQ+FIT+TEKIGGRLG SIRGTLQSQAKAFVD QHESRMTK+KAVLDQETW E
Sbjct: 601  FMSIYDLTQEFITSTEKIGGRLGSSIRGTLQSQAKAFVDSQHESRMTKLKAVLDQETWDE 660

Query: 718  VDVPDEFQTVAESI-CSQELLSEKVDHAQGNMDRSYSDVAANNGDSRIVGGESLNAQQNT 777
            +DVP+EFQ++  S+  SQ L+S KVD A  N   SY         S   G    N++   
Sbjct: 661  IDVPEEFQSIISSLFASQRLISGKVDDADLN---SYHSNRLPLNGSLTSGSGDQNSELRN 720

Query: 778  EQIDSSDMSGGNTAHVKPTPA-DTVEKSNADVTNTTTQVNNTNVKEHGKSSSQTLQYKGV 837
            E+ +SS+ S  + A VKPT + +++E+S A V++ T   N +N K HGKS+   L Y+GV
Sbjct: 721  EKSESSEGSVVSDAQVKPTVSPESLERSKAGVSSATN--NQSNQKAHGKSN---LFYQGV 780

Query: 838  GYHMVNCGLILLKMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVS 897
            GYHMVNCGLILLKMLSEYIDMN+SLPALSSE+V RVVE+L+FFNTRTCQLVLGAGAMQVS
Sbjct: 781  GYHMVNCGLILLKMLSEYIDMNNSLPALSSEIVLRVVEVLRFFNTRTCQLVLGAGAMQVS 840

Query: 898  GLKSITSKHLALASQVISFTFAIVPEIRRILFLKVPEARKTMLLSEIDRVALDFKVHRDE 957
            GLKSI +KHLALASQVI FT+ I+PE RRILF KVPE RK +L  EID+VA DF++HRDE
Sbjct: 841  GLKSIKAKHLALASQVIDFTYTIIPETRRILFSKVPETRKPLLSVEIDKVAQDFRIHRDE 900

Query: 958  IHTKLVQIMRERLSVHLRGLPQIVESWNRLENSDPQPSQFARSLTKEVGYLQRVLSRTLH 1017
            I+TKLVQIMRERL  HL GLP++VE WNR  +++ Q  +FA  LT+EVGYL RVLS TLH
Sbjct: 901  IYTKLVQIMRERLLAHLHGLPKVVEGWNRPPDTNKQTKEFAWPLTREVGYLHRVLSETLH 960

Query: 1018 EADVQAIFRQVVKIFHLQISEAFSRLDISTPQSKDRLLRDVKHILGCIRSLPSDNSSKPD 1077
            EADVQAIFRQV+ I H Q S+  + L+IS+ ++K RL   V+ IL CIRSLPSDN+++ D
Sbjct: 961  EADVQAIFRQVISIIHTQTSQTLTNLEISSTEAKKRLKLHVELILKCIRSLPSDNANQSD 1020

Query: 1078 IPNWGQLDEFFDQRFGSE 1084
            IPNWGQLDEFF + F  E
Sbjct: 1021 IPNWGQLDEFFAEHFREE 1028

BLAST of CmoCh03G014960 vs. ExPASy Swiss-Prot
Match: Q5SPW0 (Vacuolar protein sorting-associated protein 54 OS=Mus musculus OX=10090 GN=Vps54 PE=1 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 1.9e-60
Identity = 247/1006 (24.55%), Postives = 429/1006 (42.64%), Query Frame = 0

Query: 98   SSQSLSSILNNPHAGKSDASWV----GWWSSPSTVSPPEFMPLASTIASSEVTRLDFNSY 157
            S  +L + LN+P   K ++ +     G     + V P  ++P        ++++ +F +Y
Sbjct: 69   SKVNLPAALNDPTLAKRESDFFTKTWGLDFVDTEVIPSLYLP--------QISKENFIAY 128

Query: 158  TAVISDPFNRFEDIRNHSSKEDGGLDSIGGQGEALVACLREVPALYFKEDFALEDGATFR 217
               IS      E  +N    +D    ++    +     L +VP ++ K DFAL+D  TF 
Sbjct: 129  QQEISQREKIHERCKNICPPKDTFDRTLLHIHDKSRTDLEQVPKIFMKPDFALDDSLTFN 188

Query: 218  AACPF-----------LNVSQNFVLQEKLSHYLDVVELHLVKEISLRSNSFFEAQGQLQD 277
            +  P+            + + + +LQEKLSHYLD+VE+++  +ISLRS +FF A     +
Sbjct: 189  SVLPWSHFNTAGGKGSRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHE 248

Query: 278  LNVKIVEGCSRIRQIKETIRLLDVDLVDSAREIQAQNATRNNLLALQHKLKLILYVNQAI 337
            L   + +    ++ +++ I  +D  + + + +I     TRNN + + +KLKL+  V+Q  
Sbjct: 249  LQDYLKKTTQAVKMLRDKIAQIDKVMCEGSLQILRLALTRNNCVKVYNKLKLMATVHQTQ 308

Query: 338  SALKLLVASADCAGALDVTDDLLHLLEGDELAGLHCFRHLRDHVAASIESITSILSAEFM 397
              +++L+++++  GALD+      +L+  EL G+H FRHL   +    + I  ++ AEF 
Sbjct: 309  PTVQVLLSTSEFVGALDLIATTQEVLQ-QELQGIHSFRHLGSQLCELEKLIDKMMIAEFS 368

Query: 398  RASIHDAGDVDIVIITETKARASNLMNGKDEQVKLDEEETSNFCDHLLPIIIGLLRTAKL 457
              S                   S+L    + + ++ EEE       L+ ++ GLL+  KL
Sbjct: 369  TYS------------------HSDLNRPLEGECQVLEEE------RLVSLVFGLLKQRKL 428

Query: 458  PSVLRLYRDAVTADMKTAFKNAVAELLPVLLLRPLDSDFAPGERTVDADGGGASLASKLR 517
             + L +Y + +    K   K  V  +  V  +  +D+D                LA ++R
Sbjct: 429  -NFLEIYGEEMIITAKNIIKERV--INKVSQIEEIDTDVV------------VKLADQMR 488

Query: 518  GLSSEGFVQLLSAILKIVQVHLVRAAEVKKSIEWIMCNL--------------------- 577
             L+   ++ LL  I     V L R       I  ++ ++                     
Sbjct: 489  MLNFPQWIDLLKDIFSKFTVFLQRVKATLNIIHSVVLSVLEKSQRTRELEEIPQQRSAGK 548

Query: 578  -------------DGHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKV-- 637
                         +G + +D+ +      AA   T+Q + +      PH    ++  V  
Sbjct: 549  DSSLDTDVAYLTHEGWFISDAFSEGEPASAAVDTTSQRNTS------PHSEPCSSDSVSE 608

Query: 638  -------TSSQGKENEAANPSNMSRNFRADV---------LRENTEAVFA-ACDAAHGRW 697
                   +SS+ + +  A P  +      D+         L  N + +   A D  H R 
Sbjct: 609  PECTTDSSSSKEQTSACAPPGGIEIIVSEDMRLTDLELGKLASNIQELLCNASDVCHDRA 668

Query: 698  AKLLGVRTL--VHPKLRLQEFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDF 757
             K L  R       KL   EF+++  + + FI  TE+I GR   S+ G LQSQA  FV+ 
Sbjct: 669  VKFLMSRAKDGFLEKLNSTEFIALSRLMETFIVDTEQICGRKSTSLLGALQSQANKFVNR 728

Query: 758  QHESRMTKIKAVLDQETWVEVDVPDEFQTVAESICSQELLSEKVDHAQGNMDRSYSDVAA 817
             HE R TK+  +LD E W + DVP EFQ + +SI                          
Sbjct: 729  FHEERRTKLSLLLDNERWKQADVPAEFQDLVDSI-------------------------- 788

Query: 818  NNGDSRIVGGESLNAQQNTEQIDSSDMSGGNTAHVKPTPADTVEKSNADVTNTTTQVNNT 877
                                       + G  A  +  P  T E+  ADV          
Sbjct: 789  ---------------------------ADGKIALPEKKPVVTEERKPADV---------- 848

Query: 878  NVKEHGKSSSQTLQYKGVGYHMVNCGLILLKMLSEYIDMNDSLPALSSEVVHRVVEILKF 937
                        L  +G  Y +V   L+L++++ EY    D++P+++++++ R+ ++LK+
Sbjct: 849  ------------LVVEGHQYAVVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLTDLLKY 908

Query: 938  FNTRTCQLVLGAGAMQVSGLKSITSKHLALASQVISFTFAIVPEIRRILFLKVPEARKTM 997
            FN+R+CQLVLGAGA+QV GLK+IT+K+LAL+S+ +      +P IR     ++P  + +M
Sbjct: 909  FNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPVIRAHFEARLPPKQWSM 934

Query: 998  LLSEIDRVALDFKVHRDEIHTKLVQIMRERLSVHLRGLPQIVESWNRLENSDPQPSQFAR 1034
             L   D +  D+  H  EI  KLV IM       L          ++ E   P PS   R
Sbjct: 969  -LRHFDHITKDYHDHIAEISAKLVAIMDSLFDKLL----------SKYEVKAPVPSPCFR 934

BLAST of CmoCh03G014960 vs. ExPASy Swiss-Prot
Match: Q9JMK8 (Vacuolar protein sorting-associated protein 54 OS=Rattus norvegicus OX=10116 GN=Vps54 PE=2 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 4.6e-59
Identity = 245/1006 (24.35%), Postives = 426/1006 (42.35%), Query Frame = 0

Query: 98   SSQSLSSILNNPHAGKSDASWV----GWWSSPSTVSPPEFMPLASTIASSEVTRLDFNSY 157
            S  +L + LN+P   K ++ +     G     + V P  ++P        ++++  F +Y
Sbjct: 57   SKVNLPAALNDPTLAKRESDFFTKTWGLGFVDTEVIPSLYLP--------QISKEHFIAY 116

Query: 158  TAVISDPFNRFEDIRNHSSKEDGGLDSIGGQGEALVACLREVPALYFKEDFALEDGATFR 217
               IS      E  +N    +D    ++    +     L +VP ++ K DFAL+D  TF 
Sbjct: 117  QQEISQREKIHERCKNICPPKDTFDRTLLHIHDKSRTDLEQVPKIFMKPDFALDDSLTFN 176

Query: 218  AACPF-----------LNVSQNFVLQEKLSHYLDVVELHLVKEISLRSNSFFEAQGQLQD 277
            +  P+            + + + +LQEKLSHYLD+VE+++  +ISLRS +FF A     +
Sbjct: 177  SVLPWSHFNTAGGKGNRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHE 236

Query: 278  LNVKIVEGCSRIRQIKETIRLLDVDLVDSAREIQAQNATRNNLLALQHKLKLILYVNQAI 337
            L   + +    ++ +++ I  +D  + + + +I     TRNN + + +KLKL+  V+Q  
Sbjct: 237  LQDYLKKTSQAVKMLRDKIAQIDKVMCEGSLQILRLALTRNNCVKVSNKLKLMATVHQTQ 296

Query: 338  SALKLLVASADCAGALDVTDDLLHLLEGDELAGLHCFRHLRDHVAASIESITSILSAEFM 397
              +++L+++++  GALD+      +L+  EL G+H FRHL   +    + I  ++ AEF 
Sbjct: 297  PTVQVLLSTSEFVGALDLIATTQEVLQ-QELQGVHSFRHLGSQLCELEKLIDKMMIAEFS 356

Query: 398  RASIHDAGDVDIVIITETKARASNLMNGKDEQVKLDEEETSNFCDHLLPIIIGLLRTAKL 457
              S                   S+L    + + ++ EEE       L+ ++ GLL   KL
Sbjct: 357  TYS------------------HSDLNRPLEGECQVLEEE------RLVSLVFGLLEQRKL 416

Query: 458  PSVLRLYRDAVTADMKTAFKNAVAELLPVLLLRPLDSDFAPGERTVDADGGGASLASKLR 517
             + L +Y +      K   K  V  +  V  +  +D+D                LA ++R
Sbjct: 417  -NFLEIYGEETIITAKNIIKECV--INKVAQVEEIDTDAV------------VKLADQMR 476

Query: 518  GLSSEGFVQLLSAILKIVQVHLVRAAEVKKSIEWIMCNL--------------------- 577
             L+   ++ LL  I     + L R       I  ++ ++                     
Sbjct: 477  MLNFPQWIDLLKDIFSKFTIFLQRVKATLNVIHSVVLSVLDKNQRTRELEEVSQQRSAGK 536

Query: 578  -------------DGHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKVT- 637
                         +G + +D+ + A    AA   T+Q + +      PH    ++  V+ 
Sbjct: 537  DNSLDTEVAYLTHEGLFISDAFSEAEPASAAVDTTSQRNTS------PHSEPCSSDSVSE 596

Query: 638  ------SSQGKENEAANPS------NMSRNFRADVLR------ENTEAVFAACDAAHGRW 697
                  SS  KE   A+ +       +S + R   L          E +  A D  H R 
Sbjct: 597  PECTTDSSSSKEQTPASATLGGVDIIVSEDMRLTDLELGKLASNIQELLCNASDVCHDRA 656

Query: 698  AKLLGVRTL--VHPKLRLQEFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDF 757
             K L  R       KL   EF+++  + + +I  TE+I GR   S+ G LQSQA  FV+ 
Sbjct: 657  VKFLMSRAKDGFLEKLNSTEFIALSRLMETYIVDTEQICGRKSTSLLGALQSQANKFVNR 716

Query: 758  QHESRMTKIKAVLDQETWVEVDVPDEFQTVAESICSQELLSEKVDHAQGNMDRSYSDVAA 817
             HE R TK+  +LD E W + DVP EFQ + +SI                          
Sbjct: 717  FHEERRTKLSLLLDNERWKQADVPAEFQDLVDSI-------------------------- 776

Query: 818  NNGDSRIVGGESLNAQQNTEQIDSSDMSGGNTAHVKPTPADTVEKSNADVTNTTTQVNNT 877
                                       + G  A     PA T ++  ADV          
Sbjct: 777  ---------------------------ADGKIALPDKKPAATEDRKPADV---------- 836

Query: 878  NVKEHGKSSSQTLQYKGVGYHMVNCGLILLKMLSEYIDMNDSLPALSSEVVHRVVEILKF 937
                        L  +G  Y +V   L+L++++ EY    D++P+++++++ R+ ++LK+
Sbjct: 837  ------------LVVEGHQYAVVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLTDLLKY 896

Query: 938  FNTRTCQLVLGAGAMQVSGLKSITSKHLALASQVISFTFAIVPEIRRILFLKVPEARKTM 997
            FN+R+CQLVLGAGA+QV GLK+IT+K+LAL+S+ +      +P IR     ++P  ++  
Sbjct: 897  FNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPVIRAHFEARLPR-KQWS 922

Query: 998  LLSEIDRVALDFKVHRDEIHTKLVQIMRERLSVHLRGLPQIVESWNRLENSDPQPSQFAR 1034
            LL   D +  D+  H  EI +KL+ IM       L          +R E   P PS   R
Sbjct: 957  LLRHFDHITKDYHDHIGEISSKLIAIMDSLFDKLL----------SRCEVEAPAPSPCFR 922

BLAST of CmoCh03G014960 vs. ExPASy Swiss-Prot
Match: Q9P1Q0 (Vacuolar protein sorting-associated protein 54 OS=Homo sapiens OX=9606 GN=VPS54 PE=1 SV=2)

HSP 1 Score: 229.2 bits (583), Expect = 2.3e-58
Identity = 243/1000 (24.30%), Postives = 422/1000 (42.20%), Query Frame = 0

Query: 98   SSQSLSSILNNPHAGKSDASWV----GWWSSPSTVSPPEFMPLASTIASSEVTRLDFNSY 157
            S  +L + LN+P   K ++ +     G     + V P  ++P        ++++  F  Y
Sbjct: 69   SKVNLPAALNDPRLAKRESDFFTKTWGLDFVDTEVIPSFYLP--------QISKEHFTVY 128

Query: 158  TAVISDPFNRFEDIRNHSSKEDGGLDSIGGQGEALVACLREVPALYFKEDFALEDGATFR 217
               IS      E  +N    +D    ++    +     L +VP ++ K DFAL+D  TF 
Sbjct: 129  QQEISQREKIHERCKNICPPKDTFERTLLHTHDKSRTDLEQVPKIFMKPDFALDDSLTFN 188

Query: 218  AACPF-----------LNVSQNFVLQEKLSHYLDVVELHLVKEISLRSNSFFEAQGQLQD 277
            +  P+            + + + +LQEKLSHYLD+VE+++  +ISLRS +FF A     +
Sbjct: 189  SVLPWSHFNTAGGKGNRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHE 248

Query: 278  LNVKIVEGCSRIRQIKETIRLLDVDLVDSAREIQAQNATRNNLLALQHKLKLILYVNQAI 337
            L   + +    ++ +++ I  +D  + + +  I     TRNN + + +KLKL+  V+Q  
Sbjct: 249  LQDYLRKTSQAVKMLRDKIAQIDKVMCEGSLHILRLALTRNNCVKVYNKLKLMATVHQTQ 308

Query: 338  SALKLLVASADCAGALDVTDDLLHLLEGDELAGLHCFRHLRDHVAASIESITSILSAEFM 397
              +++L+++++  GALD+      +L+  EL G+H FRHL   +    + I  ++ AEF 
Sbjct: 309  PTVQVLLSTSEFVGALDLIATTQEVLQ-QELQGIHSFRHLGSQLCELEKLIDKMMIAEFS 368

Query: 398  RASIHDAGDVDIVIITETKARASNLMNGKDEQVKLDEEETSNFCDHLLPIIIGLLRTAKL 457
              S                   S+L    ++  ++ EEE       L+ ++ GLL+  KL
Sbjct: 369  TYS------------------HSDLNRPLEDDCQVLEEE------RLISLVFGLLKQRKL 428

Query: 458  PSVLRLYRDAVTADMKTAFKNAVAELLPVLLLRPLDSDFAPGERTVDADGGGASLASKLR 517
             + L +Y + +    K   K  V  +  V     +D+D                LA ++R
Sbjct: 429  -NFLEIYGEKMVITAKNIIKQCV--INKVSQTEEIDTDVV------------VKLADQMR 488

Query: 518  GLSSEGFVQLLSAILKIVQVHLVRAAEVKKSIEWIMCNL--------------------- 577
             L+   +  LL  I     + L R       I  ++ ++                     
Sbjct: 489  MLNFPQWFDLLKDIFSKFTIFLQRVKATLNIIHSVVLSVLDKNQRTRELEEISQQKNAAK 548

Query: 578  -------------DGHYAADSVAAAIVIGAAASGTAQ--DSDNQGGLLLPHLPQ-QAAAK 637
                         +G + +D+     +   A   T+Q   S N        + + +    
Sbjct: 549  DNSLDTEVAYLIHEGMFISDAFGEGELTPIAVDTTSQRNASPNSEPCSSDSVSEPECTTD 608

Query: 638  VTSSQGKENEAANPSNMSRNFRADV---------LREN-TEAVFAACDAAHGRWAKLLGV 697
             +SS+   + +A P  +      D+         L  N  E +++A D  H R  K L  
Sbjct: 609  SSSSKEHTSSSAIPGGVDIMVSEDMKLTDSELGKLANNIQELLYSASDICHDRAVKFLMS 668

Query: 698  RTL--VHPKLRLQEFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRM 757
            R       KL   EF+++  + + FI  TE+I GR   S+ G LQSQA  FV+  HE R 
Sbjct: 669  RAKDGFLEKLNSMEFITLSRLMETFILDTEQICGRKSTSLLGALQSQAIKFVNRFHEERK 728

Query: 758  TKIKAVLDQETWVEVDVPDEFQTVAESICSQELLSEKVDHAQGNMDRSYSDVAANNGDSR 817
            TK+  +LD E W + DVP EFQ + +S                                 
Sbjct: 729  TKLSLLLDNERWKQADVPAEFQDLVDS--------------------------------- 788

Query: 818  IVGGESLNAQQNTEQIDSSDMSGGNTAHVKPTPADTVEKSNADVTNTTTQVNNTNVKEHG 877
                                +S G  A  +     T E+  A+V                
Sbjct: 789  --------------------LSDGKIALPEKKSGATEERKPAEV---------------- 848

Query: 878  KSSSQTLQYKGVGYHMVNCGLILLKMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTC 937
                  L  +G  Y +V   L+L++++ EY    D++P+++++++ R+ ++LK+FN+R+C
Sbjct: 849  ------LIVEGQQYAVVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLSDLLKYFNSRSC 908

Query: 938  QLVLGAGAMQVSGLKSITSKHLALASQVISFTFAIVPEIRRILFLKVPEARKTMLLSEID 997
            QLVLGAGA+QV GLK+IT+K+LAL+S+ +      +P IR     ++P  + +M L   D
Sbjct: 909  QLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPVIRAHFEARLPPKQYSM-LRHFD 934

Query: 998  RVALDFKVHRDEIHTKLVQIMRERLSVHLRGLPQIVESWNRLENSDPQPSQFARSLTKEV 1034
             +  D+  H  EI  KLV IM       L          ++ E   P PS   R++ K++
Sbjct: 969  HITKDYHDHIAEISAKLVAIMDSLFDKLL----------SKYEVKAPVPSACFRNICKQM 934

BLAST of CmoCh03G014960 vs. ExPASy Swiss-Prot
Match: Q9VLC0 (Vacuolar protein sorting-associated protein 54 OS=Drosophila melanogaster OX=7227 GN=scat PE=1 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 1.2e-46
Identity = 235/994 (23.64%), Postives = 398/994 (40.04%), Query Frame = 0

Query: 66   GRSPTDYSSLISRETRLDRTISSPSAKSNTDASSQSLSSILNNPHAGKSDASWVGWWS-- 125
            G S  DY + +++      T   P  +    +++Q+L ++LN+P  GK    +   W   
Sbjct: 94   GVSDRDYDAHVAKYHVNQHTREMP-PEWGVYSAAQNLPAVLNDPSRGKQSNLFTKKWGEH 153

Query: 126  --SPSTVSPPEFMPLASTIASSEVTRLDFNSYTAVISDPF----NRFEDIRNHSSKEDGG 185
                S V P   +P        ++T  DF  Y   I   +     R + +      E+G 
Sbjct: 154  FVERSHVPPSPRLP--------DITHADFTVYLGSIGKRYRWHERRQQQLERDKPLENGA 213

Query: 186  LDSIG-GQGEALVACLREVPALYFKEDFALEDGATFR----------AACPFLNVSQNFV 245
              + G G G      L  VP ++ K    L   ATF+          A+ P  +      
Sbjct: 214  QGAPGPGTGGQTPTHLSSVPEIFLKSQLQLHHPATFKQVFPNYMQTSASSPESHQQTGRQ 273

Query: 246  LQEKLSHYLDVVELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQIKETI-RLLD 305
            LQE+LSHYLD+VE+ + +++S +S +FF A      +  ++ +   ++RQ++  +  L  
Sbjct: 274  LQEQLSHYLDMVEVKIAQQVSQKSAAFFHAMTTQHAILAEMEQAADQVRQLRAALAELHS 333

Query: 306  VDLVDSAREIQAQNATRNNLLALQHKLKLILYVNQAISALKLLVASADCAGALDVTDDLL 365
              +VDS + ++     ++  L L  KL+L+  V++    L+LL+ + D   ALD+     
Sbjct: 334  HSVVDSFKVLRFAQRRQHYNLTLD-KLRLMATVHKTQPMLQLLLGTQDYVAALDLIGTTQ 393

Query: 366  HLLEGDELAGLHCFRHLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKARAS 425
             +L   EL G+HCF+HL   ++   + I  +L+ EF R   + A D++  +    +    
Sbjct: 394  EILSA-ELLGIHCFKHLPMQLSEMEKLIDKMLTTEFER---YAAADLNRPLTDALR---- 453

Query: 426  NLMNGKDEQVKLDEEETSNFC---DHLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAFK 485
                           ET + C   D L+ I++GLLR     S ++ Y+    A ++   K
Sbjct: 454  ---------------ETDSVCAEEDKLVAIVMGLLRKQNF-SFVQAYQQEAIATIRAIIK 513

Query: 486  NAVAELLPVLLLRPLDSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAILKIVQV 545
              + E     +L   DSD     + +   G G                Q L   L     
Sbjct: 514  QLLIE-----VLARSDSD-----QEISLTGHGE---------------QALELTLP---- 573

Query: 546  HLVRAAEVKKSIEWIMCNLDGHYAADSVAAAI--VIGAAASGTAQDSDNQGGLLLPHLPQ 605
                        EWI        A  S+   I  V+G                    + Q
Sbjct: 574  ------------EWIALLQRSSQALVSILERIKTVVG--------------------IMQ 633

Query: 606  QAAAKVTSSQGKENEAANPSNMSRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRTLVH 665
            Q A     +Q   N   + + +S      +  +  + + A C   H R A ++  ++L  
Sbjct: 634  QTADAAVGAQDAVNLIDSEAFLSPGHHEQLKNQLQQLLQAVCHYCHERCANIVSPQSLER 693

Query: 666  PKLRLQEFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVL 725
                 QE   +  I   F   T  I G     ++  L+ QA  +    H  R  K+  +L
Sbjct: 694  SSASEQELFQLSEIVDHFGETTRSICGVASVPLQLALKVQASRYAQRFHSERKQKLSLLL 753

Query: 726  DQETWVEVDVPDEFQTVAESICSQELLSEKVDHAQGNMDRSYSDVAANNGDSRIVGGESL 785
            DQE W +VD+P EFQ + E + +        D+A+  M    S+ A N            
Sbjct: 754  DQERWRQVDIPHEFQRIIERMAAG-------DYAKPEMGNLISNGAGN------------ 813

Query: 786  NAQQNTEQIDSSDMSGGNTAHVKPTPADTVEKSNADVTNTTTQVNNTNVKEHGKSSSQTL 845
                                     P   VE                     GK      
Sbjct: 814  -------------------------PVLLVE---------------------GKQP---- 873

Query: 846  QYKGVGYHMVNCGLILLKMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAG 905
                  Y +V+  L+L++ML EY      LP L+S     VV++L+ FN+R+CQL++GAG
Sbjct: 874  ------YTLVSASLMLIRMLYEYGCSAHRLPLLASYHARNVVDLLRCFNSRSCQLIIGAG 897

Query: 906  AMQVSGLKSITSKHLALASQVISFTFAIVPEIRRILFLKVPEARKTMLLSEIDRVALDFK 965
            AM+V+GLK+ITS +LAL S+ +     ++P+++               +S  + +  D++
Sbjct: 934  AMRVAGLKTITSTNLALVSRALQLVLWLLPKLK----------EHFQAMSGYETIERDYQ 897

Query: 966  VHRDEIHTKLVQIMRERLSVHLRGLPQIVESWNRLENSDPQPSQFARSLTKEVGYLQRVL 1025
             H  EI  K+  I+ ERL+  L       ++W   E   P PSQ  R +++ +  L   +
Sbjct: 994  GHIKEIENKIHGIVSERLAAQL-------DAW---EARPPIPSQTFRHISRHLVKLHEAI 897

Query: 1026 SRTLHEADVQAIFRQVVKIFHLQISEAFSRLDIS 1035
            +  L EA +  I+  V + F  ++ E   +L+++
Sbjct: 1054 AGVLPEAQIHEIYGVVHRNFKDKLREQLLKLNVN 897

BLAST of CmoCh03G014960 vs. ExPASy TrEMBL
Match: A0A6J1ED73 (vacuolar protein sorting-associated protein 54, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111433106 PE=3 SV=1)

HSP 1 Score: 1963.3 bits (5085), Expect = 0.0e+00
Identity = 1028/1028 (100.00%), Postives = 1028/1028 (100.00%), Query Frame = 0

Query: 58   MESQPSQSGRSPTDYSSLISRETRLDRTISSPSAKSNTDASSQSLSSILNNPHAGKSDAS 117
            MESQPSQSGRSPTDYSSLISRETRLDRTISSPSAKSNTDASSQSLSSILNNPHAGKSDAS
Sbjct: 1    MESQPSQSGRSPTDYSSLISRETRLDRTISSPSAKSNTDASSQSLSSILNNPHAGKSDAS 60

Query: 118  WVGWWSSPSTVSPPEFMPLASTIASSEVTRLDFNSYTAVISDPFNRFEDIRNHSSKEDGG 177
            WVGWWSSPSTVSPPEFMPLASTIASSEVTRLDFNSYTAVISDPFNRFEDIRNHSSKEDGG
Sbjct: 61   WVGWWSSPSTVSPPEFMPLASTIASSEVTRLDFNSYTAVISDPFNRFEDIRNHSSKEDGG 120

Query: 178  LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVSQNFVLQEKLSHYLDV 237
            LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVSQNFVLQEKLSHYLDV
Sbjct: 121  LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVSQNFVLQEKLSHYLDV 180

Query: 238  VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQIKETIRLLDVDLVDSAREIQA 297
            VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQIKETIRLLDVDLVDSAREIQA
Sbjct: 181  VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQIKETIRLLDVDLVDSAREIQA 240

Query: 298  QNATRNNLLALQHKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLH 357
            QNATRNNLLALQHKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLH
Sbjct: 241  QNATRNNLLALQHKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLH 300

Query: 358  CFRHLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKARASNLMNGKDEQVKL 417
            CFRHLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKARASNLMNGKDEQVKL
Sbjct: 301  CFRHLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKARASNLMNGKDEQVKL 360

Query: 418  DEEETSNFCDHLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAFKNAVAELLPVLLLRPL 477
            DEEETSNFCDHLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAFKNAVAELLPVLLLRPL
Sbjct: 361  DEEETSNFCDHLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAFKNAVAELLPVLLLRPL 420

Query: 478  DSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAILKIVQVHLVRAAEVKKSIEWI 537
            DSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAILKIVQVHLVRAAEVKKSIEWI
Sbjct: 421  DSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAILKIVQVHLVRAAEVKKSIEWI 480

Query: 538  MCNLDGHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKVTSSQGKENEAA 597
            MCNLDGHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKVTSSQGKENEAA
Sbjct: 481  MCNLDGHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKVTSSQGKENEAA 540

Query: 598  NPSNMSRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRTLVHPKLRLQEFLSIYNITQD 657
            NPSNMSRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRTLVHPKLRLQEFLSIYNITQD
Sbjct: 541  NPSNMSRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRTLVHPKLRLQEFLSIYNITQD 600

Query: 658  FITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVEVDVPDEFQTV 717
            FITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVEVDVPDEFQTV
Sbjct: 601  FITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVEVDVPDEFQTV 660

Query: 718  AESICSQELLSEKVDHAQGNMDRSYSDVAANNGDSRIVGGESLNAQQNTEQIDSSDMSGG 777
            AESICSQELLSEKVDHAQGNMDRSYSDVAANNGDSRIVGGESLNAQQNTEQIDSSDMSGG
Sbjct: 661  AESICSQELLSEKVDHAQGNMDRSYSDVAANNGDSRIVGGESLNAQQNTEQIDSSDMSGG 720

Query: 778  NTAHVKPTPADTVEKSNADVTNTTTQVNNTNVKEHGKSSSQTLQYKGVGYHMVNCGLILL 837
            NTAHVKPTPADTVEKSNADVTNTTTQVNNTNVKEHGKSSSQTLQYKGVGYHMVNCGLILL
Sbjct: 721  NTAHVKPTPADTVEKSNADVTNTTTQVNNTNVKEHGKSSSQTLQYKGVGYHMVNCGLILL 780

Query: 838  KMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLAL 897
            KMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLAL
Sbjct: 781  KMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLAL 840

Query: 898  ASQVISFTFAIVPEIRRILFLKVPEARKTMLLSEIDRVALDFKVHRDEIHTKLVQIMRER 957
            ASQVISFTFAIVPEIRRILFLKVPEARKTMLLSEIDRVALDFKVHRDEIHTKLVQIMRER
Sbjct: 841  ASQVISFTFAIVPEIRRILFLKVPEARKTMLLSEIDRVALDFKVHRDEIHTKLVQIMRER 900

Query: 958  LSVHLRGLPQIVESWNRLENSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFRQVV 1017
            LSVHLRGLPQIVESWNRLENSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFRQVV
Sbjct: 901  LSVHLRGLPQIVESWNRLENSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFRQVV 960

Query: 1018 KIFHLQISEAFSRLDISTPQSKDRLLRDVKHILGCIRSLPSDNSSKPDIPNWGQLDEFFD 1077
            KIFHLQISEAFSRLDISTPQSKDRLLRDVKHILGCIRSLPSDNSSKPDIPNWGQLDEFFD
Sbjct: 961  KIFHLQISEAFSRLDISTPQSKDRLLRDVKHILGCIRSLPSDNSSKPDIPNWGQLDEFFD 1020

Query: 1078 QRFGSEAG 1086
            QRFGSEAG
Sbjct: 1021 QRFGSEAG 1028

BLAST of CmoCh03G014960 vs. ExPASy TrEMBL
Match: A0A6J1ECN3 (vacuolar protein sorting-associated protein 54, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111433106 PE=3 SV=1)

HSP 1 Score: 1956.8 bits (5068), Expect = 0.0e+00
Identity = 1027/1028 (99.90%), Postives = 1027/1028 (99.90%), Query Frame = 0

Query: 58   MESQPSQSGRSPTDYSSLISRETRLDRTISSPSAKSNTDASSQSLSSILNNPHAGKSDAS 117
            MESQPSQSGRSPTDYSSLISRETRLDRTISSPSAKSNTDASSQSLSSILNNPHAGKSDAS
Sbjct: 1    MESQPSQSGRSPTDYSSLISRETRLDRTISSPSAKSNTDASSQSLSSILNNPHAGKSDAS 60

Query: 118  WVGWWSSPSTVSPPEFMPLASTIASSEVTRLDFNSYTAVISDPFNRFEDIRNHSSKEDGG 177
            WVGWWSSPSTVSPPEFMPLASTIASSEVTRLDFNSYTAVISDPFNRFEDIRNHSSKEDGG
Sbjct: 61   WVGWWSSPSTVSPPEFMPLASTIASSEVTRLDFNSYTAVISDPFNRFEDIRNHSSKEDGG 120

Query: 178  LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVSQNFVLQEKLSHYLDV 237
            LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVSQNFVLQEKLSHYLDV
Sbjct: 121  LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVSQNFVLQEKLSHYLDV 180

Query: 238  VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQIKETIRLLDVDLVDSAREIQA 297
            VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQIKETIRLLDVDLVDSAREIQA
Sbjct: 181  VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQIKETIRLLDVDLVDSAREIQA 240

Query: 298  QNATRNNLLALQHKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLH 357
            QNATRNNLLALQHKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLH
Sbjct: 241  QNATRNNLLALQHKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLH 300

Query: 358  CFRHLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKARASNLMNGKDEQVKL 417
            CFRHLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKARASNLMNGKDE VKL
Sbjct: 301  CFRHLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKARASNLMNGKDE-VKL 360

Query: 418  DEEETSNFCDHLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAFKNAVAELLPVLLLRPL 477
            DEEETSNFCDHLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAFKNAVAELLPVLLLRPL
Sbjct: 361  DEEETSNFCDHLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAFKNAVAELLPVLLLRPL 420

Query: 478  DSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAILKIVQVHLVRAAEVKKSIEWI 537
            DSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAILKIVQVHLVRAAEVKKSIEWI
Sbjct: 421  DSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAILKIVQVHLVRAAEVKKSIEWI 480

Query: 538  MCNLDGHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKVTSSQGKENEAA 597
            MCNLDGHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKVTSSQGKENEAA
Sbjct: 481  MCNLDGHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKVTSSQGKENEAA 540

Query: 598  NPSNMSRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRTLVHPKLRLQEFLSIYNITQD 657
            NPSNMSRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRTLVHPKLRLQEFLSIYNITQD
Sbjct: 541  NPSNMSRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRTLVHPKLRLQEFLSIYNITQD 600

Query: 658  FITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVEVDVPDEFQTV 717
            FITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVEVDVPDEFQTV
Sbjct: 601  FITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVEVDVPDEFQTV 660

Query: 718  AESICSQELLSEKVDHAQGNMDRSYSDVAANNGDSRIVGGESLNAQQNTEQIDSSDMSGG 777
            AESICSQELLSEKVDHAQGNMDRSYSDVAANNGDSRIVGGESLNAQQNTEQIDSSDMSGG
Sbjct: 661  AESICSQELLSEKVDHAQGNMDRSYSDVAANNGDSRIVGGESLNAQQNTEQIDSSDMSGG 720

Query: 778  NTAHVKPTPADTVEKSNADVTNTTTQVNNTNVKEHGKSSSQTLQYKGVGYHMVNCGLILL 837
            NTAHVKPTPADTVEKSNADVTNTTTQVNNTNVKEHGKSSSQTLQYKGVGYHMVNCGLILL
Sbjct: 721  NTAHVKPTPADTVEKSNADVTNTTTQVNNTNVKEHGKSSSQTLQYKGVGYHMVNCGLILL 780

Query: 838  KMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLAL 897
            KMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLAL
Sbjct: 781  KMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLAL 840

Query: 898  ASQVISFTFAIVPEIRRILFLKVPEARKTMLLSEIDRVALDFKVHRDEIHTKLVQIMRER 957
            ASQVISFTFAIVPEIRRILFLKVPEARKTMLLSEIDRVALDFKVHRDEIHTKLVQIMRER
Sbjct: 841  ASQVISFTFAIVPEIRRILFLKVPEARKTMLLSEIDRVALDFKVHRDEIHTKLVQIMRER 900

Query: 958  LSVHLRGLPQIVESWNRLENSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFRQVV 1017
            LSVHLRGLPQIVESWNRLENSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFRQVV
Sbjct: 901  LSVHLRGLPQIVESWNRLENSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFRQVV 960

Query: 1018 KIFHLQISEAFSRLDISTPQSKDRLLRDVKHILGCIRSLPSDNSSKPDIPNWGQLDEFFD 1077
            KIFHLQISEAFSRLDISTPQSKDRLLRDVKHILGCIRSLPSDNSSKPDIPNWGQLDEFFD
Sbjct: 961  KIFHLQISEAFSRLDISTPQSKDRLLRDVKHILGCIRSLPSDNSSKPDIPNWGQLDEFFD 1020

Query: 1078 QRFGSEAG 1086
            QRFGSEAG
Sbjct: 1021 QRFGSEAG 1027

BLAST of CmoCh03G014960 vs. ExPASy TrEMBL
Match: A0A6J1IKB8 (vacuolar protein sorting-associated protein 54, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111477866 PE=3 SV=1)

HSP 1 Score: 1925.6 bits (4987), Expect = 0.0e+00
Identity = 1011/1028 (98.35%), Postives = 1018/1028 (99.03%), Query Frame = 0

Query: 58   MESQPSQSGRSPTDYSSLISRETRLDRTISSPSAKSNTDASSQSLSSILNNPHAGKSDAS 117
            MESQPSQSGRSPTDYSSLISRETRLDRT SSPSAKSNTDASSQSLSSILNNPHAGKSDAS
Sbjct: 1    MESQPSQSGRSPTDYSSLISRETRLDRTTSSPSAKSNTDASSQSLSSILNNPHAGKSDAS 60

Query: 118  WVGWWSSPSTVSPPEFMPLASTIASSEVTRLDFNSYTAVISDPFNRFEDIRNHSSKEDGG 177
            WVGWWSSPST+SPPEFMPLASTIASSEVTRLDFNSYTAVISDPFNRFEDIRNHSSKEDGG
Sbjct: 61   WVGWWSSPSTMSPPEFMPLASTIASSEVTRLDFNSYTAVISDPFNRFEDIRNHSSKEDGG 120

Query: 178  LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVSQNFVLQEKLSHYLDV 237
            LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACP LNVSQNFVLQEKLSHYLDV
Sbjct: 121  LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPLLNVSQNFVLQEKLSHYLDV 180

Query: 238  VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQIKETIRLLDVDLVDSAREIQA 297
            VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQ+KETIRLLDVDLVDSAREIQA
Sbjct: 181  VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQA 240

Query: 298  QNATRNNLLALQHKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLH 357
            QNATRNNLLALQHKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLH
Sbjct: 241  QNATRNNLLALQHKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLH 300

Query: 358  CFRHLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKARASNLMNGKDEQVKL 417
            CFRHLRDHVAASIESITSILSAEFMRASIHDAGDVDIVI TET+ARASNLMNGKDEQVKL
Sbjct: 301  CFRHLRDHVAASIESITSILSAEFMRASIHDAGDVDIVITTETRARASNLMNGKDEQVKL 360

Query: 418  DEEETSNFCDHLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAFKNAVAELLPVLLLRPL 477
            DEEETSNFCDHLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAFKNAVAELLPVLLLRPL
Sbjct: 361  DEEETSNFCDHLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAFKNAVAELLPVLLLRPL 420

Query: 478  DSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAILKIVQVHLVRAAEVKKSIEWI 537
            DSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAILKIVQVHLVRAAEVKKSI WI
Sbjct: 421  DSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAILKIVQVHLVRAAEVKKSIGWI 480

Query: 538  MCNLDGHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKVTSSQGKENEAA 597
            MCNLDGHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKVTSSQGKENEAA
Sbjct: 481  MCNLDGHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKVTSSQGKENEAA 540

Query: 598  NPSNMSRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRTLVHPKLRLQEFLSIYNITQD 657
            NPSNMSRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRTLVHPKLRLQEFLSIYNITQD
Sbjct: 541  NPSNMSRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRTLVHPKLRLQEFLSIYNITQD 600

Query: 658  FITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVEVDVPDEFQTV 717
            FITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVEVDVPDEFQTV
Sbjct: 601  FITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVEVDVPDEFQTV 660

Query: 718  AESICSQELLSEKVDHAQGNMDRSYSDVAANNGDSRIVGGESLNAQQNTEQIDSSDMSGG 777
            AESICSQELLSEKV+HAQGNMDRSYSDVAANN DSRIVGGESLNAQQN+EQIDSSDMSGG
Sbjct: 661  AESICSQELLSEKVEHAQGNMDRSYSDVAANNDDSRIVGGESLNAQQNSEQIDSSDMSGG 720

Query: 778  NTAHVKPTPADTVEKSNADVTNTTTQVNNTNVKEHGKSSSQTLQYKGVGYHMVNCGLILL 837
            NTAHVKPTPADTVEKSNADVTNTTTQVNN NVKE GKSSS+TLQYKGVGYHMVNCGLILL
Sbjct: 721  NTAHVKPTPADTVEKSNADVTNTTTQVNNNNVKERGKSSSKTLQYKGVGYHMVNCGLILL 780

Query: 838  KMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLAL 897
            KMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLAL
Sbjct: 781  KMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLAL 840

Query: 898  ASQVISFTFAIVPEIRRILFLKVPEARKTMLLSEIDRVALDFKVHRDEIHTKLVQIMRER 957
            ASQVISFTFAIVPEIRRILFLKVPEARKTMLLSEIDRVALDFKVHRDEIHTKLVQIMRER
Sbjct: 841  ASQVISFTFAIVPEIRRILFLKVPEARKTMLLSEIDRVALDFKVHRDEIHTKLVQIMRER 900

Query: 958  LSVHLRGLPQIVESWNRLENSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFRQVV 1017
            LSVHLRGLPQIVESWNR ENSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFRQVV
Sbjct: 901  LSVHLRGLPQIVESWNRPENSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFRQVV 960

Query: 1018 KIFHLQISEAFSRLDISTPQSKDRLLRDVKHILGCIRSLPSDNSSKPDIPNWGQLDEFFD 1077
            KIFHLQISEAFSRLDISTPQSKDRLLRDVKHILGCIRSLP  +SSKPDIPNWGQLDEFFD
Sbjct: 961  KIFHLQISEAFSRLDISTPQSKDRLLRDVKHILGCIRSLP--DSSKPDIPNWGQLDEFFD 1020

Query: 1078 QRFGSEAG 1086
            QRFGSEAG
Sbjct: 1021 QRFGSEAG 1026

BLAST of CmoCh03G014960 vs. ExPASy TrEMBL
Match: A0A6J1IIW7 (vacuolar protein sorting-associated protein 54, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111477866 PE=3 SV=1)

HSP 1 Score: 1919.1 bits (4970), Expect = 0.0e+00
Identity = 1010/1028 (98.25%), Postives = 1017/1028 (98.93%), Query Frame = 0

Query: 58   MESQPSQSGRSPTDYSSLISRETRLDRTISSPSAKSNTDASSQSLSSILNNPHAGKSDAS 117
            MESQPSQSGRSPTDYSSLISRETRLDRT SSPSAKSNTDASSQSLSSILNNPHAGKSDAS
Sbjct: 1    MESQPSQSGRSPTDYSSLISRETRLDRTTSSPSAKSNTDASSQSLSSILNNPHAGKSDAS 60

Query: 118  WVGWWSSPSTVSPPEFMPLASTIASSEVTRLDFNSYTAVISDPFNRFEDIRNHSSKEDGG 177
            WVGWWSSPST+SPPEFMPLASTIASSEVTRLDFNSYTAVISDPFNRFEDIRNHSSKEDGG
Sbjct: 61   WVGWWSSPSTMSPPEFMPLASTIASSEVTRLDFNSYTAVISDPFNRFEDIRNHSSKEDGG 120

Query: 178  LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVSQNFVLQEKLSHYLDV 237
            LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACP LNVSQNFVLQEKLSHYLDV
Sbjct: 121  LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPLLNVSQNFVLQEKLSHYLDV 180

Query: 238  VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQIKETIRLLDVDLVDSAREIQA 297
            VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQ+KETIRLLDVDLVDSAREIQA
Sbjct: 181  VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQA 240

Query: 298  QNATRNNLLALQHKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLH 357
            QNATRNNLLALQHKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLH
Sbjct: 241  QNATRNNLLALQHKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLH 300

Query: 358  CFRHLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKARASNLMNGKDEQVKL 417
            CFRHLRDHVAASIESITSILSAEFMRASIHDAGDVDIVI TET+ARASNLMNGKDE VKL
Sbjct: 301  CFRHLRDHVAASIESITSILSAEFMRASIHDAGDVDIVITTETRARASNLMNGKDE-VKL 360

Query: 418  DEEETSNFCDHLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAFKNAVAELLPVLLLRPL 477
            DEEETSNFCDHLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAFKNAVAELLPVLLLRPL
Sbjct: 361  DEEETSNFCDHLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAFKNAVAELLPVLLLRPL 420

Query: 478  DSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAILKIVQVHLVRAAEVKKSIEWI 537
            DSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAILKIVQVHLVRAAEVKKSI WI
Sbjct: 421  DSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAILKIVQVHLVRAAEVKKSIGWI 480

Query: 538  MCNLDGHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKVTSSQGKENEAA 597
            MCNLDGHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKVTSSQGKENEAA
Sbjct: 481  MCNLDGHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKVTSSQGKENEAA 540

Query: 598  NPSNMSRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRTLVHPKLRLQEFLSIYNITQD 657
            NPSNMSRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRTLVHPKLRLQEFLSIYNITQD
Sbjct: 541  NPSNMSRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRTLVHPKLRLQEFLSIYNITQD 600

Query: 658  FITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVEVDVPDEFQTV 717
            FITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVEVDVPDEFQTV
Sbjct: 601  FITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVEVDVPDEFQTV 660

Query: 718  AESICSQELLSEKVDHAQGNMDRSYSDVAANNGDSRIVGGESLNAQQNTEQIDSSDMSGG 777
            AESICSQELLSEKV+HAQGNMDRSYSDVAANN DSRIVGGESLNAQQN+EQIDSSDMSGG
Sbjct: 661  AESICSQELLSEKVEHAQGNMDRSYSDVAANNDDSRIVGGESLNAQQNSEQIDSSDMSGG 720

Query: 778  NTAHVKPTPADTVEKSNADVTNTTTQVNNTNVKEHGKSSSQTLQYKGVGYHMVNCGLILL 837
            NTAHVKPTPADTVEKSNADVTNTTTQVNN NVKE GKSSS+TLQYKGVGYHMVNCGLILL
Sbjct: 721  NTAHVKPTPADTVEKSNADVTNTTTQVNNNNVKERGKSSSKTLQYKGVGYHMVNCGLILL 780

Query: 838  KMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLAL 897
            KMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLAL
Sbjct: 781  KMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLAL 840

Query: 898  ASQVISFTFAIVPEIRRILFLKVPEARKTMLLSEIDRVALDFKVHRDEIHTKLVQIMRER 957
            ASQVISFTFAIVPEIRRILFLKVPEARKTMLLSEIDRVALDFKVHRDEIHTKLVQIMRER
Sbjct: 841  ASQVISFTFAIVPEIRRILFLKVPEARKTMLLSEIDRVALDFKVHRDEIHTKLVQIMRER 900

Query: 958  LSVHLRGLPQIVESWNRLENSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFRQVV 1017
            LSVHLRGLPQIVESWNR ENSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFRQVV
Sbjct: 901  LSVHLRGLPQIVESWNRPENSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFRQVV 960

Query: 1018 KIFHLQISEAFSRLDISTPQSKDRLLRDVKHILGCIRSLPSDNSSKPDIPNWGQLDEFFD 1077
            KIFHLQISEAFSRLDISTPQSKDRLLRDVKHILGCIRSLP  +SSKPDIPNWGQLDEFFD
Sbjct: 961  KIFHLQISEAFSRLDISTPQSKDRLLRDVKHILGCIRSLP--DSSKPDIPNWGQLDEFFD 1020

Query: 1078 QRFGSEAG 1086
            QRFGSEAG
Sbjct: 1021 QRFGSEAG 1025

BLAST of CmoCh03G014960 vs. ExPASy TrEMBL
Match: A0A6J1CXK3 (vacuolar protein sorting-associated protein 54, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111015746 PE=3 SV=1)

HSP 1 Score: 1771.9 bits (4588), Expect = 0.0e+00
Identity = 930/1028 (90.47%), Postives = 969/1028 (94.26%), Query Frame = 0

Query: 58   MESQPSQSGRSPTDYSSLISRETRLDRTISSPSAKSNTDASSQSLSSILNNPHAGKSDAS 117
            MESQPSQSGRSPT+YS+L+SRET L RT SS   K+N+DASSQSLSSILNNPHAGKSDAS
Sbjct: 1    MESQPSQSGRSPTEYSTLLSRETSLRRTTSS---KTNSDASSQSLSSILNNPHAGKSDAS 60

Query: 118  WVGWWSSPSTVSPPEFMPLASTIASSEVTRLDFNSYTAVISDPFNRFEDIRNHSSKEDGG 177
            W  WWSS STVSPPEF+PL+STIASSEVTR DFN+YTA+ISD ++RFEDIRNHS+KE  G
Sbjct: 61   WAAWWSSSSTVSPPEFLPLSSTIASSEVTRFDFNNYTALISDSYHRFEDIRNHSTKESVG 120

Query: 178  LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVSQNFVLQEKLSHYLDV 237
            LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNV+QN VLQEKLSHYLDV
Sbjct: 121  LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQEKLSHYLDV 180

Query: 238  VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQIKETIRLLDVDLVDSAREIQA 297
            VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQ+KETIRLLDVDLVDSAREIQ 
Sbjct: 181  VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQE 240

Query: 298  QNATRNNLLALQHKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLH 357
            QNATRNNLLALQ KLKLILYVNQAISALKLLVAS DCAGALDVTDDLLHLLEGDEL+GLH
Sbjct: 241  QNATRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLH 300

Query: 358  CFRHLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKARASNLMNGKDEQVKL 417
            CFRHLRDHVAASIESITSILSAEFMRAS HDAGDVD+VII ETKARASNLMNGKDE VK 
Sbjct: 301  CFRHLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDE-VKF 360

Query: 418  DEEETSNFCDHLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAFKNAVAELLPVLLLRPL 477
            DEEE SNF D LLPIIIGLLRTAKLPSVLRLYRDAVTADMKTA KNAVAELLPVLL+RPL
Sbjct: 361  DEEEASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPL 420

Query: 478  DSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAILKIVQVHLVRAAEVKKSIEWI 537
            DSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAI KIVQVHLVRAAEVKKSIEWI
Sbjct: 421  DSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWI 480

Query: 538  MCNLDGHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKVTSSQGKENEAA 597
            MCNLDGHYAADSVAAAI IGAAA+GTAQDSDNQ GLLLPHLPQ+ AAKVTS  GK N+AA
Sbjct: 481  MCNLDGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAA 540

Query: 598  NPSNMSRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRTLVHPKLRLQEFLSIYNITQD 657
            NPSNMSRNFRADVLRENTEAVFAACDAAHGRWAKLLGVR LVHPKLRLQEFLSIYNITQD
Sbjct: 541  NPSNMSRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQEFLSIYNITQD 600

Query: 658  FITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVEVDVPDEFQTV 717
            FITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVEVDVPDEFQ +
Sbjct: 601  FITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVEVDVPDEFQAI 660

Query: 718  AESICSQELLSEKVDHAQGNMDRSYSDVAANNGDSRIVGGESLNAQQNTEQIDSSDMSGG 777
            AES+ SQELLS K+D AQGNMDRSY+DV  NN DS IVGG SLNAQQ++E  DSSDM+GG
Sbjct: 661  AESLYSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNAQQHSELTDSSDMTGG 720

Query: 778  NTAHVKPTPADTVEKSNADVTNTTTQVNNTNVKEHGKSSSQTLQYKGVGYHMVNCGLILL 837
            NT H KPTPAD +EKS ADV   TTQ+NNTNVKE GKSSSQTLQYKG+GYHMVNCGLILL
Sbjct: 721  NTEHAKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQYKGIGYHMVNCGLILL 780

Query: 838  KMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLAL 897
            KMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLAL
Sbjct: 781  KMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLAL 840

Query: 898  ASQVISFTFAIVPEIRRILFLKVPEARKTMLLSEIDRVALDFKVHRDEIHTKLVQIMRER 957
            ASQVISFT AI+PEIRRILFLKVPEARKT+L+SEIDRVA D+KVHRDEIHTKLVQIMRER
Sbjct: 841  ASQVISFTCAIIPEIRRILFLKVPEARKTLLISEIDRVAQDYKVHRDEIHTKLVQIMRER 900

Query: 958  LSVHLRGLPQIVESWNRLENSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFRQVV 1017
            L VHLRGLPQIVESWNRL++SDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIF+QVV
Sbjct: 901  LLVHLRGLPQIVESWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFKQVV 960

Query: 1018 KIFHLQISEAFSRLDISTPQSKDRLLRDVKHILGCIRSLPSDNSSKPDIPNWGQLDEFFD 1077
            KIFHLQISEAFSRLDISTPQ+KDRLLRDVKHILGCIRSLP D+ SKPD PNWGQLDEF D
Sbjct: 961  KIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPDTPNWGQLDEFLD 1020

Query: 1078 QRFGSEAG 1086
            QRFGSEAG
Sbjct: 1021 QRFGSEAG 1024

BLAST of CmoCh03G014960 vs. NCBI nr
Match: KAG6581519.1 (Vacuolar protein sorting-associated protein 54, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2019.2 bits (5230), Expect = 0.0e+00
Identity = 1061/1083 (97.97%), Postives = 1063/1083 (98.15%), Query Frame = 0

Query: 3    EIWGQRQLLSSSLYSFSICLRSIPNTSSLSLAFLNSTVECDLWNPPLVHGSKNLSMESQP 62
            E+  +RQLLSSSLYSFSICLRSIPNTSSLSLAFLNSTVECDLWNPPLVHGSKNLSMESQP
Sbjct: 653  EVGRERQLLSSSLYSFSICLRSIPNTSSLSLAFLNSTVECDLWNPPLVHGSKNLSMESQP 712

Query: 63   SQSGRSPTDYSSLISRETRLDRTISSPSAKSNTDASSQSLSSILNNPHAGKSDASWVGWW 122
            SQSGRSPTDYSSLISRETRLDRTISSPSAKSNTDASSQSLSSILNNPHAGKSDASWVGWW
Sbjct: 713  SQSGRSPTDYSSLISRETRLDRTISSPSAKSNTDASSQSLSSILNNPHAGKSDASWVGWW 772

Query: 123  SSPSTVSPPEFMPLASTIASSEVTRLDFNSYTAVISDPFNRFEDIRNHSSKEDGGLDSIG 182
            SSPSTVSPPEFMPLASTIASSEVTRLDFNSYTAVISDPFNRFEDIRNHSSKEDGGLDSIG
Sbjct: 773  SSPSTVSPPEFMPLASTIASSEVTRLDFNSYTAVISDPFNRFEDIRNHSSKEDGGLDSIG 832

Query: 183  GQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVSQNFVLQEKLSHYLDVVELHL 242
            GQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVSQNFVLQEKLSHYLDVVELHL
Sbjct: 833  GQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVSQNFVLQEKLSHYLDVVELHL 892

Query: 243  VKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQIKETIRLLDVDLVDSAREIQAQNATR 302
            VKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQIKETIRLLDVDLVDSAREIQAQNATR
Sbjct: 893  VKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQIKETIRLLDVDLVDSAREIQAQNATR 952

Query: 303  NNLLALQHKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLHCFRHL 362
            NNLLALQHKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLHCFRHL
Sbjct: 953  NNLLALQHKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLHCFRHL 1012

Query: 363  RDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKARASNLMNGKDEQVKLDEEET 422
            RDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKARASNLMNGKDEQVKLDEEET
Sbjct: 1013 RDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKARASNLMNGKDEQVKLDEEET 1072

Query: 423  SNFCDHLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAFKNAVAELLPVLLLRPLDSDFA 482
            SNFCDHLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAFKNAVAELLPVLLLRPLDSDFA
Sbjct: 1073 SNFCDHLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAFKNAVAELLPVLLLRPLDSDFA 1132

Query: 483  PGERTVDADGGGASLASKLRGLSSEGFVQLLSAILKIVQVHLVRAAEVKKSIEWIMCNLD 542
            PGERTVDADGGGASLASKLRGLSSEGFVQLLSAILKIVQVHLVRAAEVKKSIEWIMCNLD
Sbjct: 1133 PGERTVDADGGGASLASKLRGLSSEGFVQLLSAILKIVQVHLVRAAEVKKSIEWIMCNLD 1192

Query: 543  GHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKVTSSQGKENEAANPSNM 602
            GHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKVTSSQGKENEAANPSNM
Sbjct: 1193 GHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKVTSSQGKENEAANPSNM 1252

Query: 603  SRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRTLVHPKLRLQEFLSIYNITQDFITAT 662
            SRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRTLVHPKLRLQEFLSIYNITQDFITAT
Sbjct: 1253 SRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRTLVHPKLRLQEFLSIYNITQDFITAT 1312

Query: 663  EKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVEVDVPDEFQTVAESIC 722
            EKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVEVDVPDEFQTVAESIC
Sbjct: 1313 EKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVEVDVPDEFQTVAESIC 1372

Query: 723  SQELLSEKVDHAQGNMDRSYSDVAANNGDSRIVGGESLNAQQNTEQIDSSDMSGGNTAHV 782
            SQELLSEKVDHAQGNMDRSYSDVAANNGDSRIVGGESLNAQQNTEQIDSSDMSGGNTAHV
Sbjct: 1373 SQELLSEKVDHAQGNMDRSYSDVAANNGDSRIVGGESLNAQQNTEQIDSSDMSGGNTAHV 1432

Query: 783  KPTPADTVEKSNADVTNTTTQVNNTNVKEHGKSSSQTLQYKGVGYHMVNCGLILLKMLSE 842
            KPTPADTVEKSNADVTNTTTQVNNTNVKEHGKSSSQTLQYKGVGYHMVNCGLILLKMLSE
Sbjct: 1433 KPTPADTVEKSNADVTNTTTQVNNTNVKEHGKSSSQTLQYKGVGYHMVNCGLILLKMLSE 1492

Query: 843  YIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLALASQVI 902
            YIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLALASQVI
Sbjct: 1493 YIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLALASQVI 1552

Query: 903  SFTFAIVPEIRRILFLKVPEARKTMLLSEIDRVALDFKVHRDEIHTKLVQIMRERLSVHL 962
            SFTFAIVPEIRRILFLK                  DFKVHRDEIHTKLVQIMRERLSVHL
Sbjct: 1553 SFTFAIVPEIRRILFLK------------------DFKVHRDEIHTKLVQIMRERLSVHL 1612

Query: 963  RGLPQIVESWNRLENSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFRQVVKIFHL 1022
            RGLPQIVESWNRLENSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFRQVVKIFHL
Sbjct: 1613 RGLPQIVESWNRLENSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFRQVVKIFHL 1672

Query: 1023 QISEAFSRLDISTPQSKDRLLRDVKHILGCIRSLPSDNSSKPDIPNWGQLDEFFDQRFGS 1082
            QISEAFSRLDISTPQSKDRLLRDVKHILGCIRSLPSDNSSKPDIPNWGQLDEFFDQRFGS
Sbjct: 1673 QISEAFSRLDISTPQSKDRLLRDVKHILGCIRSLPSDNSSKPDIPNWGQLDEFFDQRFGS 1717

Query: 1083 EAG 1086
            EAG
Sbjct: 1733 EAG 1717

BLAST of CmoCh03G014960 vs. NCBI nr
Match: XP_022925811.1 (vacuolar protein sorting-associated protein 54, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1963.3 bits (5085), Expect = 0.0e+00
Identity = 1028/1028 (100.00%), Postives = 1028/1028 (100.00%), Query Frame = 0

Query: 58   MESQPSQSGRSPTDYSSLISRETRLDRTISSPSAKSNTDASSQSLSSILNNPHAGKSDAS 117
            MESQPSQSGRSPTDYSSLISRETRLDRTISSPSAKSNTDASSQSLSSILNNPHAGKSDAS
Sbjct: 1    MESQPSQSGRSPTDYSSLISRETRLDRTISSPSAKSNTDASSQSLSSILNNPHAGKSDAS 60

Query: 118  WVGWWSSPSTVSPPEFMPLASTIASSEVTRLDFNSYTAVISDPFNRFEDIRNHSSKEDGG 177
            WVGWWSSPSTVSPPEFMPLASTIASSEVTRLDFNSYTAVISDPFNRFEDIRNHSSKEDGG
Sbjct: 61   WVGWWSSPSTVSPPEFMPLASTIASSEVTRLDFNSYTAVISDPFNRFEDIRNHSSKEDGG 120

Query: 178  LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVSQNFVLQEKLSHYLDV 237
            LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVSQNFVLQEKLSHYLDV
Sbjct: 121  LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVSQNFVLQEKLSHYLDV 180

Query: 238  VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQIKETIRLLDVDLVDSAREIQA 297
            VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQIKETIRLLDVDLVDSAREIQA
Sbjct: 181  VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQIKETIRLLDVDLVDSAREIQA 240

Query: 298  QNATRNNLLALQHKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLH 357
            QNATRNNLLALQHKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLH
Sbjct: 241  QNATRNNLLALQHKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLH 300

Query: 358  CFRHLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKARASNLMNGKDEQVKL 417
            CFRHLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKARASNLMNGKDEQVKL
Sbjct: 301  CFRHLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKARASNLMNGKDEQVKL 360

Query: 418  DEEETSNFCDHLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAFKNAVAELLPVLLLRPL 477
            DEEETSNFCDHLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAFKNAVAELLPVLLLRPL
Sbjct: 361  DEEETSNFCDHLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAFKNAVAELLPVLLLRPL 420

Query: 478  DSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAILKIVQVHLVRAAEVKKSIEWI 537
            DSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAILKIVQVHLVRAAEVKKSIEWI
Sbjct: 421  DSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAILKIVQVHLVRAAEVKKSIEWI 480

Query: 538  MCNLDGHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKVTSSQGKENEAA 597
            MCNLDGHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKVTSSQGKENEAA
Sbjct: 481  MCNLDGHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKVTSSQGKENEAA 540

Query: 598  NPSNMSRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRTLVHPKLRLQEFLSIYNITQD 657
            NPSNMSRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRTLVHPKLRLQEFLSIYNITQD
Sbjct: 541  NPSNMSRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRTLVHPKLRLQEFLSIYNITQD 600

Query: 658  FITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVEVDVPDEFQTV 717
            FITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVEVDVPDEFQTV
Sbjct: 601  FITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVEVDVPDEFQTV 660

Query: 718  AESICSQELLSEKVDHAQGNMDRSYSDVAANNGDSRIVGGESLNAQQNTEQIDSSDMSGG 777
            AESICSQELLSEKVDHAQGNMDRSYSDVAANNGDSRIVGGESLNAQQNTEQIDSSDMSGG
Sbjct: 661  AESICSQELLSEKVDHAQGNMDRSYSDVAANNGDSRIVGGESLNAQQNTEQIDSSDMSGG 720

Query: 778  NTAHVKPTPADTVEKSNADVTNTTTQVNNTNVKEHGKSSSQTLQYKGVGYHMVNCGLILL 837
            NTAHVKPTPADTVEKSNADVTNTTTQVNNTNVKEHGKSSSQTLQYKGVGYHMVNCGLILL
Sbjct: 721  NTAHVKPTPADTVEKSNADVTNTTTQVNNTNVKEHGKSSSQTLQYKGVGYHMVNCGLILL 780

Query: 838  KMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLAL 897
            KMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLAL
Sbjct: 781  KMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLAL 840

Query: 898  ASQVISFTFAIVPEIRRILFLKVPEARKTMLLSEIDRVALDFKVHRDEIHTKLVQIMRER 957
            ASQVISFTFAIVPEIRRILFLKVPEARKTMLLSEIDRVALDFKVHRDEIHTKLVQIMRER
Sbjct: 841  ASQVISFTFAIVPEIRRILFLKVPEARKTMLLSEIDRVALDFKVHRDEIHTKLVQIMRER 900

Query: 958  LSVHLRGLPQIVESWNRLENSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFRQVV 1017
            LSVHLRGLPQIVESWNRLENSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFRQVV
Sbjct: 901  LSVHLRGLPQIVESWNRLENSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFRQVV 960

Query: 1018 KIFHLQISEAFSRLDISTPQSKDRLLRDVKHILGCIRSLPSDNSSKPDIPNWGQLDEFFD 1077
            KIFHLQISEAFSRLDISTPQSKDRLLRDVKHILGCIRSLPSDNSSKPDIPNWGQLDEFFD
Sbjct: 961  KIFHLQISEAFSRLDISTPQSKDRLLRDVKHILGCIRSLPSDNSSKPDIPNWGQLDEFFD 1020

Query: 1078 QRFGSEAG 1086
            QRFGSEAG
Sbjct: 1021 QRFGSEAG 1028

BLAST of CmoCh03G014960 vs. NCBI nr
Match: XP_022925812.1 (vacuolar protein sorting-associated protein 54, chloroplastic-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1956.8 bits (5068), Expect = 0.0e+00
Identity = 1027/1028 (99.90%), Postives = 1027/1028 (99.90%), Query Frame = 0

Query: 58   MESQPSQSGRSPTDYSSLISRETRLDRTISSPSAKSNTDASSQSLSSILNNPHAGKSDAS 117
            MESQPSQSGRSPTDYSSLISRETRLDRTISSPSAKSNTDASSQSLSSILNNPHAGKSDAS
Sbjct: 1    MESQPSQSGRSPTDYSSLISRETRLDRTISSPSAKSNTDASSQSLSSILNNPHAGKSDAS 60

Query: 118  WVGWWSSPSTVSPPEFMPLASTIASSEVTRLDFNSYTAVISDPFNRFEDIRNHSSKEDGG 177
            WVGWWSSPSTVSPPEFMPLASTIASSEVTRLDFNSYTAVISDPFNRFEDIRNHSSKEDGG
Sbjct: 61   WVGWWSSPSTVSPPEFMPLASTIASSEVTRLDFNSYTAVISDPFNRFEDIRNHSSKEDGG 120

Query: 178  LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVSQNFVLQEKLSHYLDV 237
            LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVSQNFVLQEKLSHYLDV
Sbjct: 121  LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVSQNFVLQEKLSHYLDV 180

Query: 238  VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQIKETIRLLDVDLVDSAREIQA 297
            VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQIKETIRLLDVDLVDSAREIQA
Sbjct: 181  VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQIKETIRLLDVDLVDSAREIQA 240

Query: 298  QNATRNNLLALQHKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLH 357
            QNATRNNLLALQHKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLH
Sbjct: 241  QNATRNNLLALQHKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLH 300

Query: 358  CFRHLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKARASNLMNGKDEQVKL 417
            CFRHLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKARASNLMNGKDE VKL
Sbjct: 301  CFRHLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKARASNLMNGKDE-VKL 360

Query: 418  DEEETSNFCDHLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAFKNAVAELLPVLLLRPL 477
            DEEETSNFCDHLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAFKNAVAELLPVLLLRPL
Sbjct: 361  DEEETSNFCDHLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAFKNAVAELLPVLLLRPL 420

Query: 478  DSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAILKIVQVHLVRAAEVKKSIEWI 537
            DSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAILKIVQVHLVRAAEVKKSIEWI
Sbjct: 421  DSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAILKIVQVHLVRAAEVKKSIEWI 480

Query: 538  MCNLDGHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKVTSSQGKENEAA 597
            MCNLDGHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKVTSSQGKENEAA
Sbjct: 481  MCNLDGHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKVTSSQGKENEAA 540

Query: 598  NPSNMSRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRTLVHPKLRLQEFLSIYNITQD 657
            NPSNMSRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRTLVHPKLRLQEFLSIYNITQD
Sbjct: 541  NPSNMSRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRTLVHPKLRLQEFLSIYNITQD 600

Query: 658  FITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVEVDVPDEFQTV 717
            FITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVEVDVPDEFQTV
Sbjct: 601  FITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVEVDVPDEFQTV 660

Query: 718  AESICSQELLSEKVDHAQGNMDRSYSDVAANNGDSRIVGGESLNAQQNTEQIDSSDMSGG 777
            AESICSQELLSEKVDHAQGNMDRSYSDVAANNGDSRIVGGESLNAQQNTEQIDSSDMSGG
Sbjct: 661  AESICSQELLSEKVDHAQGNMDRSYSDVAANNGDSRIVGGESLNAQQNTEQIDSSDMSGG 720

Query: 778  NTAHVKPTPADTVEKSNADVTNTTTQVNNTNVKEHGKSSSQTLQYKGVGYHMVNCGLILL 837
            NTAHVKPTPADTVEKSNADVTNTTTQVNNTNVKEHGKSSSQTLQYKGVGYHMVNCGLILL
Sbjct: 721  NTAHVKPTPADTVEKSNADVTNTTTQVNNTNVKEHGKSSSQTLQYKGVGYHMVNCGLILL 780

Query: 838  KMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLAL 897
            KMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLAL
Sbjct: 781  KMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLAL 840

Query: 898  ASQVISFTFAIVPEIRRILFLKVPEARKTMLLSEIDRVALDFKVHRDEIHTKLVQIMRER 957
            ASQVISFTFAIVPEIRRILFLKVPEARKTMLLSEIDRVALDFKVHRDEIHTKLVQIMRER
Sbjct: 841  ASQVISFTFAIVPEIRRILFLKVPEARKTMLLSEIDRVALDFKVHRDEIHTKLVQIMRER 900

Query: 958  LSVHLRGLPQIVESWNRLENSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFRQVV 1017
            LSVHLRGLPQIVESWNRLENSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFRQVV
Sbjct: 901  LSVHLRGLPQIVESWNRLENSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFRQVV 960

Query: 1018 KIFHLQISEAFSRLDISTPQSKDRLLRDVKHILGCIRSLPSDNSSKPDIPNWGQLDEFFD 1077
            KIFHLQISEAFSRLDISTPQSKDRLLRDVKHILGCIRSLPSDNSSKPDIPNWGQLDEFFD
Sbjct: 961  KIFHLQISEAFSRLDISTPQSKDRLLRDVKHILGCIRSLPSDNSSKPDIPNWGQLDEFFD 1020

Query: 1078 QRFGSEAG 1086
            QRFGSEAG
Sbjct: 1021 QRFGSEAG 1027

BLAST of CmoCh03G014960 vs. NCBI nr
Match: XP_023543739.1 (vacuolar protein sorting-associated protein 54, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1942.5 bits (5031), Expect = 0.0e+00
Identity = 1017/1028 (98.93%), Postives = 1022/1028 (99.42%), Query Frame = 0

Query: 58   MESQPSQSGRSPTDYSSLISRETRLDRTISSPSAKSNTDASSQSLSSILNNPHAGKSDAS 117
            MESQPSQSGRSPTDYS LISRETRLDRTISSPSAKSNTDASSQSLSSILNNPHAGKSDAS
Sbjct: 1    MESQPSQSGRSPTDYSCLISRETRLDRTISSPSAKSNTDASSQSLSSILNNPHAGKSDAS 60

Query: 118  WVGWWSSPSTVSPPEFMPLASTIASSEVTRLDFNSYTAVISDPFNRFEDIRNHSSKEDGG 177
            WVGWWSSPSTVSPPE MPLASTIASSEVTRLDFNSYTAVISDPFNRFEDIRNHSSKEDGG
Sbjct: 61   WVGWWSSPSTVSPPELMPLASTIASSEVTRLDFNSYTAVISDPFNRFEDIRNHSSKEDGG 120

Query: 178  LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVSQNFVLQEKLSHYLDV 237
            LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVSQNFVLQEKLSHYLDV
Sbjct: 121  LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVSQNFVLQEKLSHYLDV 180

Query: 238  VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQIKETIRLLDVDLVDSAREIQA 297
            VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQ+KETIRLLDVDLVDSAREIQA
Sbjct: 181  VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQA 240

Query: 298  QNATRNNLLALQHKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLH 357
            QNATRNNLLALQHKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLH
Sbjct: 241  QNATRNNLLALQHKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLH 300

Query: 358  CFRHLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKARASNLMNGKDEQVKL 417
            CFRHLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKARASNLMNGKDEQVKL
Sbjct: 301  CFRHLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKARASNLMNGKDEQVKL 360

Query: 418  DEEETSNFCDHLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAFKNAVAELLPVLLLRPL 477
            DEEETSNFCDHLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAFKNAV ELLPVLLLRPL
Sbjct: 361  DEEETSNFCDHLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAFKNAVEELLPVLLLRPL 420

Query: 478  DSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAILKIVQVHLVRAAEVKKSIEWI 537
            DSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAILKIVQVHLVRAAEVKKSIEWI
Sbjct: 421  DSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAILKIVQVHLVRAAEVKKSIEWI 480

Query: 538  MCNLDGHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKVTSSQGKENEAA 597
            MCNLDGHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKVTSSQGKENEAA
Sbjct: 481  MCNLDGHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKVTSSQGKENEAA 540

Query: 598  NPSNMSRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRTLVHPKLRLQEFLSIYNITQD 657
            NPSNMSRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRTLVHPKLRLQEFLSIYNITQD
Sbjct: 541  NPSNMSRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRTLVHPKLRLQEFLSIYNITQD 600

Query: 658  FITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVEVDVPDEFQTV 717
            FITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRM KIKAVLDQETWVEVDVPDEFQTV
Sbjct: 601  FITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMAKIKAVLDQETWVEVDVPDEFQTV 660

Query: 718  AESICSQELLSEKVDHAQGNMDRSYSDVAANNGDSRIVGGESLNAQQNTEQIDSSDMSGG 777
            AESICSQELLSEKVDHAQGNMDRSYSDVAANN DSRIVGGESLNAQQN+EQIDSSDMSGG
Sbjct: 661  AESICSQELLSEKVDHAQGNMDRSYSDVAANNDDSRIVGGESLNAQQNSEQIDSSDMSGG 720

Query: 778  NTAHVKPTPADTVEKSNADVTNTTTQVNNTNVKEHGKSSSQTLQYKGVGYHMVNCGLILL 837
            NTAHVKPTPADTVEKSNADVTNTTTQVNNTNVKE GKSSSQTLQYKGVGYHMVNCGLILL
Sbjct: 721  NTAHVKPTPADTVEKSNADVTNTTTQVNNTNVKERGKSSSQTLQYKGVGYHMVNCGLILL 780

Query: 838  KMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLAL 897
            KMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLAL
Sbjct: 781  KMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLAL 840

Query: 898  ASQVISFTFAIVPEIRRILFLKVPEARKTMLLSEIDRVALDFKVHRDEIHTKLVQIMRER 957
            ASQVISFTFAIVPEIRRILFLKVP+ARKTMLLSEIDRVALDFKVHRDEIHTKLVQIMRER
Sbjct: 841  ASQVISFTFAIVPEIRRILFLKVPDARKTMLLSEIDRVALDFKVHRDEIHTKLVQIMRER 900

Query: 958  LSVHLRGLPQIVESWNRLENSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFRQVV 1017
            LSVHLRGLPQIVESWNRLENSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFRQVV
Sbjct: 901  LSVHLRGLPQIVESWNRLENSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFRQVV 960

Query: 1018 KIFHLQISEAFSRLDISTPQSKDRLLRDVKHILGCIRSLPSDNSSKPDIPNWGQLDEFFD 1077
            KIFHLQISEAFSRLDISTPQSKDRLLRDVKHILGCIRSLPSD+SSKPDIPNWG+LDEFFD
Sbjct: 961  KIFHLQISEAFSRLDISTPQSKDRLLRDVKHILGCIRSLPSDDSSKPDIPNWGKLDEFFD 1020

Query: 1078 QRFGSEAG 1086
            QRFGSEAG
Sbjct: 1021 QRFGSEAG 1028

BLAST of CmoCh03G014960 vs. NCBI nr
Match: XP_023543740.1 (vacuolar protein sorting-associated protein 54, chloroplastic-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1936.0 bits (5014), Expect = 0.0e+00
Identity = 1016/1028 (98.83%), Postives = 1021/1028 (99.32%), Query Frame = 0

Query: 58   MESQPSQSGRSPTDYSSLISRETRLDRTISSPSAKSNTDASSQSLSSILNNPHAGKSDAS 117
            MESQPSQSGRSPTDYS LISRETRLDRTISSPSAKSNTDASSQSLSSILNNPHAGKSDAS
Sbjct: 1    MESQPSQSGRSPTDYSCLISRETRLDRTISSPSAKSNTDASSQSLSSILNNPHAGKSDAS 60

Query: 118  WVGWWSSPSTVSPPEFMPLASTIASSEVTRLDFNSYTAVISDPFNRFEDIRNHSSKEDGG 177
            WVGWWSSPSTVSPPE MPLASTIASSEVTRLDFNSYTAVISDPFNRFEDIRNHSSKEDGG
Sbjct: 61   WVGWWSSPSTVSPPELMPLASTIASSEVTRLDFNSYTAVISDPFNRFEDIRNHSSKEDGG 120

Query: 178  LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVSQNFVLQEKLSHYLDV 237
            LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVSQNFVLQEKLSHYLDV
Sbjct: 121  LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVSQNFVLQEKLSHYLDV 180

Query: 238  VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQIKETIRLLDVDLVDSAREIQA 297
            VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQ+KETIRLLDVDLVDSAREIQA
Sbjct: 181  VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQA 240

Query: 298  QNATRNNLLALQHKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLH 357
            QNATRNNLLALQHKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLH
Sbjct: 241  QNATRNNLLALQHKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLH 300

Query: 358  CFRHLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKARASNLMNGKDEQVKL 417
            CFRHLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKARASNLMNGKDE VKL
Sbjct: 301  CFRHLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKARASNLMNGKDE-VKL 360

Query: 418  DEEETSNFCDHLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAFKNAVAELLPVLLLRPL 477
            DEEETSNFCDHLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAFKNAV ELLPVLLLRPL
Sbjct: 361  DEEETSNFCDHLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAFKNAVEELLPVLLLRPL 420

Query: 478  DSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAILKIVQVHLVRAAEVKKSIEWI 537
            DSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAILKIVQVHLVRAAEVKKSIEWI
Sbjct: 421  DSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAILKIVQVHLVRAAEVKKSIEWI 480

Query: 538  MCNLDGHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKVTSSQGKENEAA 597
            MCNLDGHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKVTSSQGKENEAA
Sbjct: 481  MCNLDGHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKVTSSQGKENEAA 540

Query: 598  NPSNMSRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRTLVHPKLRLQEFLSIYNITQD 657
            NPSNMSRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRTLVHPKLRLQEFLSIYNITQD
Sbjct: 541  NPSNMSRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRTLVHPKLRLQEFLSIYNITQD 600

Query: 658  FITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVEVDVPDEFQTV 717
            FITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRM KIKAVLDQETWVEVDVPDEFQTV
Sbjct: 601  FITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMAKIKAVLDQETWVEVDVPDEFQTV 660

Query: 718  AESICSQELLSEKVDHAQGNMDRSYSDVAANNGDSRIVGGESLNAQQNTEQIDSSDMSGG 777
            AESICSQELLSEKVDHAQGNMDRSYSDVAANN DSRIVGGESLNAQQN+EQIDSSDMSGG
Sbjct: 661  AESICSQELLSEKVDHAQGNMDRSYSDVAANNDDSRIVGGESLNAQQNSEQIDSSDMSGG 720

Query: 778  NTAHVKPTPADTVEKSNADVTNTTTQVNNTNVKEHGKSSSQTLQYKGVGYHMVNCGLILL 837
            NTAHVKPTPADTVEKSNADVTNTTTQVNNTNVKE GKSSSQTLQYKGVGYHMVNCGLILL
Sbjct: 721  NTAHVKPTPADTVEKSNADVTNTTTQVNNTNVKERGKSSSQTLQYKGVGYHMVNCGLILL 780

Query: 838  KMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLAL 897
            KMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLAL
Sbjct: 781  KMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLAL 840

Query: 898  ASQVISFTFAIVPEIRRILFLKVPEARKTMLLSEIDRVALDFKVHRDEIHTKLVQIMRER 957
            ASQVISFTFAIVPEIRRILFLKVP+ARKTMLLSEIDRVALDFKVHRDEIHTKLVQIMRER
Sbjct: 841  ASQVISFTFAIVPEIRRILFLKVPDARKTMLLSEIDRVALDFKVHRDEIHTKLVQIMRER 900

Query: 958  LSVHLRGLPQIVESWNRLENSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFRQVV 1017
            LSVHLRGLPQIVESWNRLENSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFRQVV
Sbjct: 901  LSVHLRGLPQIVESWNRLENSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFRQVV 960

Query: 1018 KIFHLQISEAFSRLDISTPQSKDRLLRDVKHILGCIRSLPSDNSSKPDIPNWGQLDEFFD 1077
            KIFHLQISEAFSRLDISTPQSKDRLLRDVKHILGCIRSLPSD+SSKPDIPNWG+LDEFFD
Sbjct: 961  KIFHLQISEAFSRLDISTPQSKDRLLRDVKHILGCIRSLPSDDSSKPDIPNWGKLDEFFD 1020

Query: 1078 QRFGSEAG 1086
            QRFGSEAG
Sbjct: 1021 QRFGSEAG 1027

BLAST of CmoCh03G014960 vs. TAIR 10
Match: AT4G19490.1 (VPS54 )

HSP 1 Score: 1242.3 bits (3213), Expect = 0.0e+00
Identity = 679/1038 (65.41%), Postives = 818/1038 (78.81%), Query Frame = 0

Query: 58   MESQPSQSGRSPT--DYSSL-ISRETRLDRTISSPSAKSNTDASSQSLSSILNNPHAGK- 117
            M+S PS  GRS T  + SSL + R +    +  SP  KS +DASSQSLSSILNNPH GK 
Sbjct: 1    MDSHPSLMGRSITNSNRSSLDLGRPSSSSSSSPSPLTKSISDASSQSLSSILNNPHGGKS 60

Query: 118  ----SDASWVGWWSSPSTVSPPEFMPLAST-IASSEVTRLDFNSYTAVISDPFNRFEDIR 177
                SDASWVGWWSS + V+P EF P+AST +  SE+TR DF+ Y + IS+   RFEDIR
Sbjct: 61   GVYGSDASWVGWWSSSTFVAPAEFAPVASTKLPGSELTRSDFHGYVSSISESHGRFEDIR 120

Query: 178  NHSSKEDGGLDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVSQNFVLQ 237
             H+ +E  G D        L ACLREVP+LYFKEDFALEDGATFR+ACPF ++++N  LQ
Sbjct: 121  KHTREESCGFDQ-ESHVSGLAACLREVPSLYFKEDFALEDGATFRSACPFSSLNENLALQ 180

Query: 238  EKLSHYLDVVELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQIKETIRLLDVDL 297
            EKLS YLDVVELHLVKEIS+RS+SFFEAQGQLQDLNVKIVEGCSRIR++KETIRLLD +L
Sbjct: 181  EKLSQYLDVVELHLVKEISVRSDSFFEAQGQLQDLNVKIVEGCSRIRELKETIRLLDRNL 240

Query: 298  VDSAREIQAQNATRNNLLALQHKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLL 357
            VDSAR+IQ  ++TR N+L LQ KL+LILYVNQA+SALKLLVASADCAGALD+TDDL +LL
Sbjct: 241  VDSARQIQELSSTRINMLELQRKLRLILYVNQALSALKLLVASADCAGALDITDDLQNLL 300

Query: 358  EGDELAGLHCFRHLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKARASNLM 417
             GDEL GL+CFRHLRDHV +SI+SI SIL++EFMR SIHD G++D++I++    R S   
Sbjct: 301  AGDELTGLYCFRHLRDHVTSSIDSINSILTSEFMRISIHDTGEIDVLILSAANIRGSISS 360

Query: 418  NGK-DEQVKLDEEETSNFCDHLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAFKNAVAE 477
            NG   E+VKL+EE+TS  CD LLP++IGLLRTAK PS+LR+YRD +T++MK A K AVA+
Sbjct: 361  NGNTGEEVKLEEEDTSTLCDRLLPLVIGLLRTAKFPSILRMYRDTLTSEMKNAIKKAVAD 420

Query: 478  LLPVLLLRPLDSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAILKIVQVHLVRA 537
            LLP+L+ R L+SDF+ GER+VD DGGG SLASKLR LSSE FV LL+AI KIVQ HLVRA
Sbjct: 421  LLPILVARSLESDFSHGERSVD-DGGGLSLASKLRTLSSEAFVNLLTAIFKIVQAHLVRA 480

Query: 538  AEVKKSIEWIMCNLDGHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKVT 597
            +EVKK+IEWI+CN+DGHYAADSVAAAI +GA A+ TAQ+   QGG L+     +A +K  
Sbjct: 481  SEVKKAIEWILCNIDGHYAADSVAAAIAVGAVAAETAQEIGFQGGSLVSSPLGKATSKAP 540

Query: 598  SSQGKENEAANPSNMSRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRTLVHPKLRLQE 657
              QGK ++A++  NMSRNFRADVLRENTEAVFAAC+  HGRWAKLLGVR L+HPKL+LQE
Sbjct: 541  PLQGKSSDASSLMNMSRNFRADVLRENTEAVFAACEVTHGRWAKLLGVRALLHPKLKLQE 600

Query: 658  FLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVE 717
            F+SIY++TQ+FIT+TEKIGGRLG SIRGTLQSQAKAFVD QHESRMTK+KAVLDQETW E
Sbjct: 601  FMSIYDLTQEFITSTEKIGGRLGSSIRGTLQSQAKAFVDSQHESRMTKLKAVLDQETWDE 660

Query: 718  VDVPDEFQTVAESI-CSQELLSEKVDHAQGNMDRSYSDVAANNGDSRIVGGESLNAQQNT 777
            +DVP+EFQ++  S+  SQ L+S KVD A  N   SY         S   G    N++   
Sbjct: 661  IDVPEEFQSIISSLFASQRLISGKVDDADLN---SYHSNRLPLNGSLTSGSGDQNSELRN 720

Query: 778  EQIDSSDMSGGNTAHVKPTPA-DTVEKSNADVTNTTTQVNNTNVKEHGKSSSQTLQYKGV 837
            E+ +SS+ S  + A VKPT + +++E+S A V++ T   N +N K HGKS+   L Y+GV
Sbjct: 721  EKSESSEGSVVSDAQVKPTVSPESLERSKAGVSSATN--NQSNQKAHGKSN---LFYQGV 780

Query: 838  GYHMVNCGLILLKMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVS 897
            GYHMVNCGLILLKMLSEYIDMN+SLPALSSE+V RVVE+L+FFNTRTCQLVLGAGAMQVS
Sbjct: 781  GYHMVNCGLILLKMLSEYIDMNNSLPALSSEIVLRVVEVLRFFNTRTCQLVLGAGAMQVS 840

Query: 898  GLKSITSKHLALASQVISFTFAIVPEIRRILFLKVPEARKTMLLSEIDRVALDFKVHRDE 957
            GLKSI +KHLALASQVI FT+ I+PE RRILF KVPE RK +L  EID+VA DF++HRDE
Sbjct: 841  GLKSIKAKHLALASQVIDFTYTIIPETRRILFSKVPETRKPLLSVEIDKVAQDFRIHRDE 900

Query: 958  IHTKLVQIMRERLSVHLRGLPQIVESWNRLENSDPQPSQFARSLTKEVGYLQRVLSRTLH 1017
            I+TKLVQIMRERL  HL GLP++VE WNR  +++ Q  +FA  LT+EVGYL RVLS TLH
Sbjct: 901  IYTKLVQIMRERLLAHLHGLPKVVEGWNRPPDTNKQTKEFAWPLTREVGYLHRVLSETLH 960

Query: 1018 EADVQAIFRQVVKIFHLQISEAFSRLDISTPQSKDRLLRDVKHILGCIRSLPSDNSSKPD 1077
            EADVQAIFRQV+ I H Q S+  + L+IS+ ++K RL   V+ IL CIRSLPSDN+++ D
Sbjct: 961  EADVQAIFRQVISIIHTQTSQTLTNLEISSTEAKKRLKLHVELILKCIRSLPSDNANQSD 1020

Query: 1078 IPNWGQLDEFFDQRFGSE 1084
            IPNWGQLDEFF + F  E
Sbjct: 1021 IPNWGQLDEFFAEHFREE 1028

BLAST of CmoCh03G014960 vs. TAIR 10
Match: AT4G19490.2 (VPS54 )

HSP 1 Score: 1242.3 bits (3213), Expect = 0.0e+00
Identity = 679/1038 (65.41%), Postives = 818/1038 (78.81%), Query Frame = 0

Query: 58   MESQPSQSGRSPT--DYSSL-ISRETRLDRTISSPSAKSNTDASSQSLSSILNNPHAGK- 117
            M+S PS  GRS T  + SSL + R +    +  SP  KS +DASSQSLSSILNNPH GK 
Sbjct: 1    MDSHPSLMGRSITNSNRSSLDLGRPSSSSSSSPSPLTKSISDASSQSLSSILNNPHGGKS 60

Query: 118  ----SDASWVGWWSSPSTVSPPEFMPLAST-IASSEVTRLDFNSYTAVISDPFNRFEDIR 177
                SDASWVGWWSS + V+P EF P+AST +  SE+TR DF+ Y + IS+   RFEDIR
Sbjct: 61   GVYGSDASWVGWWSSSTFVAPAEFAPVASTKLPGSELTRSDFHGYVSSISESHGRFEDIR 120

Query: 178  NHSSKEDGGLDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVSQNFVLQ 237
             H+ +E  G D        L ACLREVP+LYFKEDFALEDGATFR+ACPF ++++N  LQ
Sbjct: 121  KHTREESCGFDQ-ESHVSGLAACLREVPSLYFKEDFALEDGATFRSACPFSSLNENLALQ 180

Query: 238  EKLSHYLDVVELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQIKETIRLLDVDL 297
            EKLS YLDVVELHLVKEIS+RS+SFFEAQGQLQDLNVKIVEGCSRIR++KETIRLLD +L
Sbjct: 181  EKLSQYLDVVELHLVKEISVRSDSFFEAQGQLQDLNVKIVEGCSRIRELKETIRLLDRNL 240

Query: 298  VDSAREIQAQNATRNNLLALQHKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLL 357
            VDSAR+IQ  ++TR N+L LQ KL+LILYVNQA+SALKLLVASADCAGALD+TDDL +LL
Sbjct: 241  VDSARQIQELSSTRINMLELQRKLRLILYVNQALSALKLLVASADCAGALDITDDLQNLL 300

Query: 358  EGDELAGLHCFRHLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKARASNLM 417
             GDEL GL+CFRHLRDHV +SI+SI SIL++EFMR SIHD G++D++I++    R S   
Sbjct: 301  AGDELTGLYCFRHLRDHVTSSIDSINSILTSEFMRISIHDTGEIDVLILSAANIRGSISS 360

Query: 418  NGK-DEQVKLDEEETSNFCDHLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAFKNAVAE 477
            NG   E+VKL+EE+TS  CD LLP++IGLLRTAK PS+LR+YRD +T++MK A K AVA+
Sbjct: 361  NGNTGEEVKLEEEDTSTLCDRLLPLVIGLLRTAKFPSILRMYRDTLTSEMKNAIKKAVAD 420

Query: 478  LLPVLLLRPLDSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAILKIVQVHLVRA 537
            LLP+L+ R L+SDF+ GER+VD DGGG SLASKLR LSSE FV LL+AI KIVQ HLVRA
Sbjct: 421  LLPILVARSLESDFSHGERSVD-DGGGLSLASKLRTLSSEAFVNLLTAIFKIVQAHLVRA 480

Query: 538  AEVKKSIEWIMCNLDGHYAADSVAAAIVIGAAASGTAQDSDNQGGLLLPHLPQQAAAKVT 597
            +EVKK+IEWI+CN+DGHYAADSVAAAI +GA A+ TAQ+   QGG L+     +A +K  
Sbjct: 481  SEVKKAIEWILCNIDGHYAADSVAAAIAVGAVAAETAQEIGFQGGSLVSSPLGKATSKAP 540

Query: 598  SSQGKENEAANPSNMSRNFRADVLRENTEAVFAACDAAHGRWAKLLGVRTLVHPKLRLQE 657
              QGK ++A++  NMSRNFRADVLRENTEAVFAAC+  HGRWAKLLGVR L+HPKL+LQE
Sbjct: 541  PLQGKSSDASSLMNMSRNFRADVLRENTEAVFAACEVTHGRWAKLLGVRALLHPKLKLQE 600

Query: 658  FLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVE 717
            F+SIY++TQ+FIT+TEKIGGRLG SIRGTLQSQAKAFVD QHESRMTK+KAVLDQETW E
Sbjct: 601  FMSIYDLTQEFITSTEKIGGRLGSSIRGTLQSQAKAFVDSQHESRMTKLKAVLDQETWDE 660

Query: 718  VDVPDEFQTVAESI-CSQELLSEKVDHAQGNMDRSYSDVAANNGDSRIVGGESLNAQQNT 777
            +DVP+EFQ++  S+  SQ L+S KVD A  N   SY         S   G    N++   
Sbjct: 661  IDVPEEFQSIISSLFASQRLISGKVDDADLN---SYHSNRLPLNGSLTSGSGDQNSELRN 720

Query: 778  EQIDSSDMSGGNTAHVKPTPA-DTVEKSNADVTNTTTQVNNTNVKEHGKSSSQTLQYKGV 837
            E+ +SS+ S  + A VKPT + +++E+S A V++ T   N +N K HGKS+   L Y+GV
Sbjct: 721  EKSESSEGSVVSDAQVKPTVSPESLERSKAGVSSATN--NQSNQKAHGKSN---LFYQGV 780

Query: 838  GYHMVNCGLILLKMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVS 897
            GYHMVNCGLILLKMLSEYIDMN+SLPALSSE+V RVVE+L+FFNTRTCQLVLGAGAMQVS
Sbjct: 781  GYHMVNCGLILLKMLSEYIDMNNSLPALSSEIVLRVVEVLRFFNTRTCQLVLGAGAMQVS 840

Query: 898  GLKSITSKHLALASQVISFTFAIVPEIRRILFLKVPEARKTMLLSEIDRVALDFKVHRDE 957
            GLKSI +KHLALASQVI FT+ I+PE RRILF KVPE RK +L  EID+VA DF++HRDE
Sbjct: 841  GLKSIKAKHLALASQVIDFTYTIIPETRRILFSKVPETRKPLLSVEIDKVAQDFRIHRDE 900

Query: 958  IHTKLVQIMRERLSVHLRGLPQIVESWNRLENSDPQPSQFARSLTKEVGYLQRVLSRTLH 1017
            I+TKLVQIMRERL  HL GLP++VE WNR  +++ Q  +FA  LT+EVGYL RVLS TLH
Sbjct: 901  IYTKLVQIMRERLLAHLHGLPKVVEGWNRPPDTNKQTKEFAWPLTREVGYLHRVLSETLH 960

Query: 1018 EADVQAIFRQVVKIFHLQISEAFSRLDISTPQSKDRLLRDVKHILGCIRSLPSDNSSKPD 1077
            EADVQAIFRQV+ I H Q S+  + L+IS+ ++K RL   V+ IL CIRSLPSDN+++ D
Sbjct: 961  EADVQAIFRQVISIIHTQTSQTLTNLEISSTEAKKRLKLHVELILKCIRSLPSDNANQSD 1020

Query: 1078 IPNWGQLDEFFDQRFGSE 1084
            IPNWGQLDEFF + F  E
Sbjct: 1021 IPNWGQLDEFFAEHFREE 1028