CmoCh03G014810 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh03G014810
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Vacuolar ATPase assembly integral membrane protein VMA21 homolog
Location	Cmo_Chr03: 10634721 .. 10634987 (-)
RNA-Seq Expression	CmoCh03G014810
Synteny	CmoCh03G014810

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCGCGTGATTACATTTCTAAGTAAATACTCTTCTGGACCAGGTTCAACACAATTGAATCCGCATTCTCTTACACTCTTGAGTGGATTCCTTGCTGTCATATCAGTCAATGTTGTCATTGCTATTTATATTATCATGGCAATGAAAGAACCCTCTAGTAAACATGAACCGGATCCTGCATTTGTTGCCGAGGCAAAAGCTAGTATAAACCAGTCTCCTGGGAAAGCAGAGGACCCCTCACAGGCCAATAATAAGAAGGAAGAATAG

mRNA sequence

ATGCGCGTGATTACATTTCTAAGTAAATACTCTTCTGGACCAGGTTCAACACAATTGAATCCGCATTCTCTTACACTCTTGAGTGGATTCCTTGCTGTCATATCAGTCAATGTTGTCATTGCTATTTATATTATCATGGCAATGAAAGAACCCTCTAGTAAACATGAACCGGATCCTGCATTTGTTGCCGAGGCAAAAGCTAGTATAAACCAGTCTCCTGGGAAAGCAGAGGACCCCTCACAGGCCAATAATAAGAAGGAAGAATAG

Coding sequence (CDS)

ATGCGCGTGATTACATTTCTAAGTAAATACTCTTCTGGACCAGGTTCAACACAATTGAATCCGCATTCTCTTACACTCTTGAGTGGATTCCTTGCTGTCATATCAGTCAATGTTGTCATTGCTATTTATATTATCATGGCAATGAAAGAACCCTCTAGTAAACATGAACCGGATCCTGCATTTGTTGCCGAGGCAAAAGCTAGTATAAACCAGTCTCCTGGGAAAGCAGAGGACCCCTCACAGGCCAATAATAAGAAGGAAGAATAG

Protein sequence

MRVITFLSKYSSGPGSTQLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPAFVAEAKASINQSPGKAEDPSQANNKKEE

Homology

[Close]

BLAST of CmoCh03G014810 vs. ExPASy TrEMBL
Match: A0A6J1EJT7 (Vacuolar ATPase assembly integral membrane protein VMA21 homolog OS=Cucurbita moschata OX=3662 GN=LOC111433255 PE=3 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 4.1e-30
Identity = 75/75 (100.00%), Postives = 75/75 (100.00%), Query Frame = 0


Query: 14  PGSTQLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPAFVAEAKASINQSP 73
           PGSTQLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPAFVAEAKASINQSP
Sbjct: 32  PGSTQLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPAFVAEAKASINQSP 91

Query: 74  GKAEDPSQANNKKEE 89
           GKAEDPSQANNKKEE
Sbjct: 92  GKAEDPSQANNKKEE 106

[Close]

BLAST of CmoCh03G014810 vs. ExPASy TrEMBL
Match: A0A6J1ISV9 (Vacuolar ATPase assembly integral membrane protein VMA21 homolog OS=Cucurbita maxima OX=3661 GN=LOC111478118 PE=3 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 3.5e-29
Identity = 73/75 (97.33%), Postives = 74/75 (98.67%), Query Frame = 0


Query: 14  PGSTQLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPAFVAEAKASINQSP 73
           PGST LNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPAFVAEAKASINQ+P
Sbjct: 32  PGSTHLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPAFVAEAKASINQAP 91

Query: 74  GKAEDPSQANNKKEE 89
           GKAEDPSQANNKKEE
Sbjct: 92  GKAEDPSQANNKKEE 106

[Close]

BLAST of CmoCh03G014810 vs. ExPASy TrEMBL
Match: A0A0A0KPB7 (Vacuolar ATPase assembly integral membrane protein VMA21 homolog OS=Cucumis sativus OX=3659 GN=Csa_6G526400 PE=3 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.6e-26
Identity = 69/75 (92.00%), Postives = 71/75 (94.67%), Query Frame = 0


Query: 14  PGSTQLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPAFVAEAKASINQSP 73
           PGSTQLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDP F+AEAKASINQS 
Sbjct: 204 PGSTQLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPTFLAEAKASINQST 263

Query: 74  GKAEDPSQANNKKEE 89
           GKA+ PSQA NKKEE
Sbjct: 264 GKADGPSQAQNKKEE 278

[Close]

BLAST of CmoCh03G014810 vs. ExPASy TrEMBL
Match: A0A1S4DTT6 (Vacuolar ATPase assembly integral membrane protein VMA21 homolog OS=Cucumis melo OX=3656 GN=LOC103484273 PE=3 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 8.1e-26
Identity = 67/75 (89.33%), Postives = 72/75 (96.00%), Query Frame = 0


Query: 14  PGSTQLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPAFVAEAKASINQSP 73
           PGST+LNPHSLTLLSGFLAVISVNVVI IYIIMAMKEPSSKHEPDPAF+AEAKASINQS 
Sbjct: 32  PGSTRLNPHSLTLLSGFLAVISVNVVIVIYIIMAMKEPSSKHEPDPAFLAEAKASINQST 91

Query: 74  GKAEDPSQANNKKEE 89
           GKA+ PS+A+NKKEE
Sbjct: 92  GKADGPSEAHNKKEE 106

[Close]

BLAST of CmoCh03G014810 vs. ExPASy TrEMBL
Match: A0A5A7UE48 (Vacuolar ATPase assembly integral membrane protein VMA21 homolog OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G0010040 PE=3 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 8.1e-26
Identity = 67/75 (89.33%), Postives = 72/75 (96.00%), Query Frame = 0


Query: 14  PGSTQLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPAFVAEAKASINQSP 73
           PGST+LNPHSLTLLSGFLAVISVNVVI IYIIMAMKEPSSKHEPDPAF+AEAKASINQS 
Sbjct: 116 PGSTRLNPHSLTLLSGFLAVISVNVVIVIYIIMAMKEPSSKHEPDPAFLAEAKASINQST 175

Query: 74  GKAEDPSQANNKKEE 89
           GKA+ PS+A+NKKEE
Sbjct: 176 GKADGPSEAHNKKEE 190

[Close]

BLAST of CmoCh03G014810 vs. NCBI nr
Match: XP_022926009.1 (uncharacterized protein LOC111433255 [Cucurbita moschata] >XP_022926010.1 uncharacterized protein LOC111433255 [Cucurbita moschata] >XP_023543189.1 uncharacterized protein LOC111803138 [Cucurbita pepo subsp. pepo] >KAG7034797.1 hypothetical protein SDJN02_04529 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 140.2 bits (352), Expect = 8.6e-30
Identity = 75/75 (100.00%), Postives = 75/75 (100.00%), Query Frame = 0


Query: 14  PGSTQLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPAFVAEAKASINQSP 73
           PGSTQLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPAFVAEAKASINQSP
Sbjct: 32  PGSTQLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPAFVAEAKASINQSP 91

Query: 74  GKAEDPSQANNKKEE 89
           GKAEDPSQANNKKEE
Sbjct: 92  GKAEDPSQANNKKEE 106

[Close]

BLAST of CmoCh03G014810 vs. NCBI nr
Match: KAG6581507.1 (hypothetical protein SDJN03_21509, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 137.5 bits (345), Expect = 5.5e-29
Identity = 74/74 (100.00%), Postives = 74/74 (100.00%), Query Frame = 0


Query: 15  GSTQLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPAFVAEAKASINQSPG 74
           GSTQLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPAFVAEAKASINQSPG
Sbjct: 110 GSTQLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPAFVAEAKASINQSPG 169

Query: 75  KAEDPSQANNKKEE 89
           KAEDPSQANNKKEE
Sbjct: 170 KAEDPSQANNKKEE 183

[Close]

BLAST of CmoCh03G014810 vs. NCBI nr
Match: XP_022977993.1 (uncharacterized protein LOC111478118 [Cucurbita maxima] >XP_022977994.1 uncharacterized protein LOC111478118 [Cucurbita maxima])

HSP 1 Score: 137.1 bits (344), Expect = 7.2e-29
Identity = 73/75 (97.33%), Postives = 74/75 (98.67%), Query Frame = 0


Query: 14  PGSTQLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPAFVAEAKASINQSP 73
           PGST LNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPAFVAEAKASINQ+P
Sbjct: 32  PGSTHLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPAFVAEAKASINQAP 91

Query: 74  GKAEDPSQANNKKEE 89
           GKAEDPSQANNKKEE
Sbjct: 92  GKAEDPSQANNKKEE 106

[Close]

BLAST of CmoCh03G014810 vs. NCBI nr
Match: XP_004134542.1 (uncharacterized protein LOC101206493 [Cucumis sativus] >XP_011658340.1 uncharacterized protein LOC101206493 [Cucumis sativus] >KAE8647778.1 hypothetical protein Csa_003577 [Cucumis sativus])

HSP 1 Score: 128.3 bits (321), Expect = 3.4e-26
Identity = 69/75 (92.00%), Postives = 71/75 (94.67%), Query Frame = 0


Query: 14  PGSTQLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPAFVAEAKASINQSP 73
           PGSTQLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDP F+AEAKASINQS 
Sbjct: 32  PGSTQLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPTFLAEAKASINQST 91

Query: 74  GKAEDPSQANNKKEE 89
           GKA+ PSQA NKKEE
Sbjct: 92  GKADGPSQAQNKKEE 106

[Close]

BLAST of CmoCh03G014810 vs. NCBI nr
Match: XP_038882777.1 (protein SUPPRESSOR OF FRI 4-like isoform X1 [Benincasa hispida])

HSP 1 Score: 126.7 bits (317), Expect = 9.8e-26
Identity = 68/75 (90.67%), Postives = 71/75 (94.67%), Query Frame = 0


Query: 14  PGSTQLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPAFVAEAKASINQSP 73
           PGSTQLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPAF+ EAKASINQS 
Sbjct: 138 PGSTQLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPAFLVEAKASINQSN 197

Query: 74  GKAEDPSQANNKKEE 89
           GK  +PSQA+NKKEE
Sbjct: 198 GKLNEPSQAHNKKEE 212

[Close]

BLAST of CmoCh03G014810 vs. TAIR 10
Match: AT1G05780.1 (Vacuolar ATPase assembly integral membrane protein VMA21-like domain )

HSP 1 Score: 81.6 bits (200), Expect = 3.4e-16
Identity = 46/75 (61.33%), Postives = 54/75 (72.00%), Query Frame = 0


Query: 14  PGSTQLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPAFVAEAKASINQSP 73
           PGST L+PHSL LLSGFLAV+SVN VI  YI MAMKEP+ KH+PD AF+AEAK S+N+  
Sbjct: 32  PGSTTLSPHSLILLSGFLAVVSVNTVIVFYICMAMKEPADKHKPDAAFLAEAKDSVNKLT 91

Query: 74  GKAEDPSQANNKKEE 89
             A        KK+E
Sbjct: 92  KGATSSDDHALKKQE 106

[Close]

BLAST of CmoCh03G014810 vs. TAIR 10
Match: AT2G31710.1 (Vacuolar ATPase assembly integral membrane protein VMA21-like domain )

HSP 1 Score: 81.3 bits (199), Expect = 4.4e-16
Identity = 44/74 (59.46%), Postives = 55/74 (74.32%), Query Frame = 0


Query: 14  PGSTQLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPAFVAEAKASINQSP 73
           PGST L+PHSLTLLSGFLAV+SVNVVI  YI +A+KEP+ KH+PD +FVAEAK S+ +  
Sbjct: 32  PGSTTLSPHSLTLLSGFLAVVSVNVVIVFYICLALKEPADKHKPDASFVAEAKDSVKKLT 91

Query: 74  GKAEDPSQANNKKE 88
               +   A  K+E
Sbjct: 92  SGVTNTDPALKKQE 105

The following BLAST results are available for this feature:

BLAST of CmoCh03G014810 vs.


Match Name	E-value	Identity	Description

BLAST of CmoCh03G014810 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EJT7	4.1e-30	100.00	Vacuolar ATPase assembly integral membrane protein VMA21 homolog OS=Cucurbita mo...	[more]
A0A6J1ISV9	3.5e-29	97.33	Vacuolar ATPase assembly integral membrane protein VMA21 homolog OS=Cucurbita ma...	[more]
A0A0A0KPB7	1.6e-26	92.00	Vacuolar ATPase assembly integral membrane protein VMA21 homolog OS=Cucumis sati...	[more]
A0A1S4DTT6	8.1e-26	89.33	Vacuolar ATPase assembly integral membrane protein VMA21 homolog OS=Cucumis melo...	[more]
A0A5A7UE48	8.1e-26	89.33	Vacuolar ATPase assembly integral membrane protein VMA21 homolog OS=Cucumis melo...	[more]

BLAST of CmoCh03G014810 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022926009.1	8.6e-30	100.00	uncharacterized protein LOC111433255 [Cucurbita moschata] >XP_022926010.1 unchar...	[more]
KAG6581507.1	5.5e-29	100.00	hypothetical protein SDJN03_21509, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022977993.1	7.2e-29	97.33	uncharacterized protein LOC111478118 [Cucurbita maxima] >XP_022977994.1 uncharac...	[more]
XP_004134542.1	3.4e-26	92.00	uncharacterized protein LOC101206493 [Cucumis sativus] >XP_011658340.1 uncharact...	[more]
XP_038882777.1	9.8e-26	90.67	protein SUPPRESSOR OF FRI 4-like isoform X1 [Benincasa hispida]	[more]

BLAST of CmoCh03G014810 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G05780.1	3.4e-16	61.33	Vacuolar ATPase assembly integral membrane protein VMA21-like domain	[more]
AT2G31710.1	4.4e-16	59.46	Vacuolar ATPase assembly integral membrane protein VMA21-like domain	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019013	Vacuolar ATPase assembly integral membrane protein Vma21	PFAM	PF09446	VMA21	coord: 6..48 e-value: 7.0E-12 score: 45.3
IPR019013	Vacuolar ATPase assembly integral membrane protein Vma21	PANTHER	PTHR31792	VACUOLAR ATPASE ASSEMBLY INTEGRAL MEMBRANE PROTEIN VMA21	coord: 4..87
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 52..88
None	No IPR available	PANTHER	PTHR31792:SF3	VACUOLAR ATPASE ASSEMBLY INTEGRAL MEMBRANE PROTEIN VMA21	coord: 4..87

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh03G014810.1	CmoCh03G014810.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0070072	vacuolar proton-transporting V-type ATPase complex assembly
cellular_component	GO:0033116	endoplasmic reticulum-Golgi intermediate compartment membrane
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0012507	ER to Golgi transport vesicle membrane
cellular_component	GO:0016021	integral component of membrane