CmoCh03G013820 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh03G013820
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionNAD(P)-binding domain containing protein
LocationCmo_Chr03: 10129129 .. 10131727 (+)
RNA-Seq ExpressionCmoCh03G013820
SyntenyCmoCh03G013820
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGATTACATGGCGCTAGTAGAGCCGCGGGAAGGAGATCTGTGGCTCGGCAATGGCCTTTCTTTCCCTTCCCAACACCATCACTTCTTCCTTCTTCCAATCGAACTCTCAACGTCTCCATTTCTGTCCTCTCTGCAAATCCTTTCTCGCCGTTTCCTCCTCCTCCAAGAAGTTCGCCTCCATCTCCATTCTCAACTCCTACAAGGTAATTCTTGTCCCTATTCTATGCTGGATTTCCATTACTTCGAGTTGTTTAGGGAGTAAAATGTCACTAGCACACTATGAGGACTACTAGACATGAGTTTCCTTTTCGCTGCTCGCGTACATGATGCTGCGGAAAGGATATGAAGTCACCAGGCTTTTTTATTTTTAGGTTTTTATGAATTCTAGTGATTTATTCTAAAGTTCTATTGCCTATCGGCAGATGGAAGGAAATGAAATTACGGAAGAAGTTGCAGAGAAACAAAGCGAGGACAATTTAAATGTGAAGAAAAAGATTTTTGTGGCTGGTGCATCCGGTAGTACTGGAAAAAGGATTGTGGAGCAGTTGCTGGCAAGGGGCTTTGCAGTCAAGGCCGGGGTTCGAGACGTAAGTAAGGCTAAAACTACTCTCTCCCCAGGCAATCCAGATCTTCAAATTGTAAGTTTGTCCATTAAATTTTTTCGGAATTTCATTGGGAATTTTTTTGAATTATTTCAGTTGCAATTCAATTTTGTTAATCTTATAACAGGTAAAAGCGGATGTGACTGAAGGCTCTGCAAAGTTGGCAGAAGCCATAGGTTCCGATTCTGAAGCTGTAATCTGTGCTACGGGGTTTCGCCGAGGATGGGACTTATTTGCGCCATGGAAGGTTTGCTTTCAATGCCATTCAAATTTCTCGAATATTTTAACTTCAAATCTCAACTCTTTGTGCTTCAGTATTTTTCCTTCTTAACTGCCACGAAAGTTGCGTAGAAAATAGGTGTATTTTAAATTATCTGTTTTTACTTTCTTTTTAATTTTCTTAAAGGCACGACAAACTGGTCAAATATCACTTCTCCTTCCCACGAGTTCAAAATAAACTCTCTCGTCGAAAATTGAAGCTAAATTCAAAAAAAAGAAAAAGAAAAAAGGAACACTTTTGCAATTTTTTTTATAAAAAAAAATACTCATTCAAAAGTAACCATTTCAAATAGAAATACATTAGAAATTTTTATGAAAATTGAGACTTGGAAAGGTGGGGGCAATGATATGAAATATTGTGATGGTGTTCGGGTCTCAAATTTCATTCATGTCTCCCTTACACAATTCTAGGTTAGAAATGTTGTCCATTTATCTGATATTTCAAATCAGCAAACTAATGCCAAGGACTAAAAATGAAGGTCAGAATGTGTGATACTATCACTCATGAAGGTTGACAATTTTTTAACCACCATGGAATTGGTCTAATAACCGACGAGATCTCATGGTGTTTTTGAAGTCATAAGTGGAGTATGGTACCAAAGTATTGAAGATCCAACGAGATTTAATTAGTTGAGGTGTGTAAGTTAAAGCTTGAGCGCCCTCGATTATTAGAGATATACATATAAATACATATGCATGCATATATACATACATACACGTGTATATATATGGAAAAAGAAGAAAGTGAACAAATGTGCAGTTATTAGTAACTTTGTAAAGTGAACATTCAATGTGGTGCTATTGTTATGTACAAATCTCCGTATCTCTATTTGTTGTATTAAGAAAGGAAAATATTCTCAAAATGTTTGTGTTACTGGCAGGTTGACAATTTTGGCACTGTAAACCTTGTTCAAGCATGCCGTGAGTTGGGCATAAACAGGTTTATTCTGATCAGTTCCATTTTAGTCAATGGAGCTGCAATGGGACAGATTCTCAACCCAGCTTATATCTTTTTAAATGTTTTGGGGCTCACCTTGATTGCAAAGCTTCAGGCGGAGCAGCACATCAGGAAATCGGGTATTAACTACACAATTATAAGGCCTGGAGGTTTGAAAAATGAGCCTCCTACCGGAAATTTAGTCATGACGGCAGAAGTAATCACAGTTTTCTCTTGTTTACCTTGATCGATCATTGTTTCTCTATGACATTTAGGCGCATGACTGAATCTTGGTTGTTTTTAGGATACTCTTTATGAAGGAAGCATATCAAGAGATTTAGTTGCAGAAGTAGCTGTTGAGGCATTGGTTCATTCCCAAGCCTCTTACAAGGTTGTTGAAATAGTCTCTCGAGCCGATGCGCCAAAGCGTTCGTATGAGGATCTCTTTGGCTCCATAAAGCAGAGATAGTCATCTTCAGATGCCATCTTATCCTCGCGGAATTGAAGCGCTTGATATCTTGCTTCCTGGTGGCAGTGCCTCATTTTCCAAGCCCCCTTGGGATATTCATGCTATATTTCACTGCATTATAATGTATTGTTTCTGTTAATACATTCATTTGCTAACGTTGATGTATAGCTTGCTTTTAATGAGAACATAAAGAAAGAATTTAAGAACTGAGAAAGGAAGAAGAAACGCCCTCCAGATCACAAAACTCTGATTTTTCAGCAATAGCCACAATTTTTTTGTTTCCTGACATTGATTTTATATACAGTGGAG

mRNA sequence

ATGGGATTACATGGCGCTAGTAGAGCCGCGGGAAGGAGATCTGTGGCTCGGCAATGGCCTTTCTTTCCCTTCCCAACACCATCACTTCTTCCTTCTTCCAATCGAACTCTCAACGTCTCCATTTCTGTCCTCTCTGCAAATCCTTTCTCGCCGTTTCCTCCTCCTCCAAGAAGTTCGCCTCCATCTCCATTCTCAACTCCTACAAGTTCTATTGCCTATCGGCAGATGGAAGGAAATGAAATTACGGAAGAAGTTGCAGAGAAACAAAGCGAGGACAATTTAAATGTGAAGAAAAAGATTTTTGTGGCTGGTGCATCCGGTAGTACTGGAAAAAGGATTGTGGAGCAGTTGCTGGCAAGGGGCTTTGCAGTCAAGGCCGGGGTTCGAGACGTAAGTAAGGCTAAAACTACTCTCTCCCCAGGCAATCCAGATCTTCAAATTGTAAAAGCGGATGTGACTGAAGGCTCTGCAAAGTTGGCAGAAGCCATAGGTTCCGATTCTGAAGCTGTAATCTGTGCTACGGGGTTTCGCCGAGGATGGGACTTATTTGCGCCATGGAAGGTTGACAATTTTGGCACTGTAAACCTTGTTCAAGCATGCCGTGAGTTGGGCATAAACAGGTTTATTCTGATCAGTTCCATTTTAGTCAATGGAGCTGCAATGGGACAGATTCTCAACCCAGCTTATATCTTTTTAAATGTTTTGGGGCTCACCTTGATTGCAAAGCTTCAGGCGGAGCAGCACATCAGGAAATCGGGTATTAACTACACAATTATAAGGCCTGGAGGTTTGAAAAATGAGCCTCCTACCGGAAATTTAGTCATGACGGCAGAAGATACTCTTTATGAAGGAAGCATATCAAGAGATTTAGTTGCAGAAGTAGCTGTTGAGGCATTGGTTCATTCCCAAGCCTCTTACAAGGTTGTTGAAATAGTCTCTCGAGCCGATGCGCCAAAGCGTTCGTATGAGGATCTCTTTGGCTCCATAAAGCAGAGATAGTCATCTTCAGATGCCATCTTATCCTCGCGGAATTGAAGCGCTTGATATCTTGCTTCCTGGTGGCAGTGCCTCATTTTCCAAGCCCCCTTGGGATATTCATGCTATATTTCACTGCATTATAATGTATTGTTTCTGTTAATACATTCATTTGCTAACGTTGATGTATAGCTTGCTTTTAATGAGAACATAAAGAAAGAATTTAAGAACTGAGAAAGGAAGAAGAAACGCCCTCCAGATCACAAAACTCTGATTTTTCAGCAATAGCCACAATTTTTTTGTTTCCTGACATTGATTTTATATACAGTGGAG

Coding sequence (CDS)

ATGGGATTACATGGCGCTAGTAGAGCCGCGGGAAGGAGATCTGTGGCTCGGCAATGGCCTTTCTTTCCCTTCCCAACACCATCACTTCTTCCTTCTTCCAATCGAACTCTCAACGTCTCCATTTCTGTCCTCTCTGCAAATCCTTTCTCGCCGTTTCCTCCTCCTCCAAGAAGTTCGCCTCCATCTCCATTCTCAACTCCTACAAGTTCTATTGCCTATCGGCAGATGGAAGGAAATGAAATTACGGAAGAAGTTGCAGAGAAACAAAGCGAGGACAATTTAAATGTGAAGAAAAAGATTTTTGTGGCTGGTGCATCCGGTAGTACTGGAAAAAGGATTGTGGAGCAGTTGCTGGCAAGGGGCTTTGCAGTCAAGGCCGGGGTTCGAGACGTAAGTAAGGCTAAAACTACTCTCTCCCCAGGCAATCCAGATCTTCAAATTGTAAAAGCGGATGTGACTGAAGGCTCTGCAAAGTTGGCAGAAGCCATAGGTTCCGATTCTGAAGCTGTAATCTGTGCTACGGGGTTTCGCCGAGGATGGGACTTATTTGCGCCATGGAAGGTTGACAATTTTGGCACTGTAAACCTTGTTCAAGCATGCCGTGAGTTGGGCATAAACAGGTTTATTCTGATCAGTTCCATTTTAGTCAATGGAGCTGCAATGGGACAGATTCTCAACCCAGCTTATATCTTTTTAAATGTTTTGGGGCTCACCTTGATTGCAAAGCTTCAGGCGGAGCAGCACATCAGGAAATCGGGTATTAACTACACAATTATAAGGCCTGGAGGTTTGAAAAATGAGCCTCCTACCGGAAATTTAGTCATGACGGCAGAAGATACTCTTTATGAAGGAAGCATATCAAGAGATTTAGTTGCAGAAGTAGCTGTTGAGGCATTGGTTCATTCCCAAGCCTCTTACAAGGTTGTTGAAATAGTCTCTCGAGCCGATGCGCCAAAGCGTTCGTATGAGGATCTCTTTGGCTCCATAAAGCAGAGATAG

Protein sequence

MGLHGASRAAGRRSVARQWPFFPFPTPSLLPSSNRTLNVSISVLSANPFSPFPPPPRSSPPSPFSTPTSSIAYRQMEGNEITEEVAEKQSEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFAVKAGVRDVSKAKTTLSPGNPDLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRRGWDLFAPWKVDNFGTVNLVQACRELGINRFILISSILVNGAAMGQILNPAYIFLNVLGLTLIAKLQAEQHIRKSGINYTIIRPGGLKNEPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSQASYKVVEIVSRADAPKRSYEDLFGSIKQR
Homology
BLAST of CmoCh03G013820 vs. ExPASy Swiss-Prot
Match: Q8H124 (Uncharacterized protein At2g34460, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g34460 PE=1 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 4.7e-100
Identity = 186/262 (70.99%), Postives = 219/262 (83.59%), Query Frame = 0

Query: 69  SSIAYRQMEGNEITEEVAEKQSEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFAVKAGV 128
           SS   +       T    EK   +N    KK+FVAGA+G TGKRIVEQLL+RGFAVKAGV
Sbjct: 18  SSFFTKNKSFRSFTSIKMEKGEAENAVKTKKVFVAGATGQTGKRIVEQLLSRGFAVKAGV 77

Query: 129 RDVSKAKTTLSPGNPDLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRRGWDLFAPWKV 188
           RDV KAKT+    +P LQIV+ADVTEG  KLAE IG DS+AVICATGFR G+D+F PWKV
Sbjct: 78  RDVEKAKTSFK-DDPSLQIVRADVTEGPDKLAEVIGDDSQAVICATGFRPGFDIFTPWKV 137

Query: 189 DNFGTVNLVQACRELGINRFILISSILVNGAAMGQILNPAYIFLNVLGLTLIAKLQAEQH 248
           DNFGTVNLV ACR+ G+ +F+L+SSILVNGAAMGQILNPAY+FLN+ GLTL+AKLQAE++
Sbjct: 138 DNFGTVNLVDACRKQGVEKFVLVSSILVNGAAMGQILNPAYLFLNLFGLTLVAKLQAEKY 197

Query: 249 IRKSGINYTIIRPGGLKNEPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSQASYKV 308
           I+KSGINYTI+RPGGLKN+PPTGN+VM  EDTLYEGSISRDLVAEVAVEAL+  ++S+KV
Sbjct: 198 IKKSGINYTIVRPGGLKNDPPTGNVVMEPEDTLYEGSISRDLVAEVAVEALLQEESSFKV 257

Query: 309 VEIVSRADAPKRSYEDLFGSIK 331
           VEIV+RA+APKRSY+DLF S+K
Sbjct: 258 VEIVARAEAPKRSYKDLFASVK 278

BLAST of CmoCh03G013820 vs. ExPASy Swiss-Prot
Match: Q8H0U5 (Protein TIC 62, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC62 PE=1 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 8.5e-25
Identity = 92/271 (33.95%), Postives = 138/271 (50.92%), Query Frame = 0

Query: 85  VAEKQSEDNLNVKKK--IFVAGASGSTGKRIVEQLLARGFAVKAGVRDVSKAKTTLSP-- 144
           V  + S  NLN K++  +FVAGA+G  G R V +LL  GF V+AGVR   +A + +    
Sbjct: 67  VVTEASPTNLNSKEEDLVFVAGATGKVGSRTVRELLKLGFRVRAGVRSAQRAGSLVQSVK 126

Query: 145 ----GNPD--------LQIVKADVTEGSAKLAEAIGSDSEAVICATGF--RRGWDLFAPW 204
                N D        L+IV+ D+ E    +  A+G+ S  +IC  G   +   D+  P+
Sbjct: 127 EMKLQNTDEGTQPVEKLEIVECDL-EKKDSIQPALGNAS-VIICCIGASEKEISDITGPY 186

Query: 205 KVDNFGTVNLVQACRELGINRFILISSILVNGAAMGQILNPAYIFLNVLGLTLIAKLQAE 264
           ++D   T NLV A     +N FIL++S+  N         PA I LN+    L  K +AE
Sbjct: 187 RIDYLATKNLVDAATSAKVNNFILVTSLGTNKFGF-----PAAI-LNLFWGVLCWKRKAE 246

Query: 265 QHIRKSGINYTIIRPGGLKNE----PPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHS 324
           + + +SG+NY I+RPGG++        T NL +  +DTL+ G +S   VAE+      + 
Sbjct: 247 EALIESGLNYAIVRPGGMERPTDAYKETHNLTLALDDTLFGGQVSNLQVAELLACMAKNP 306

Query: 325 QASY-KVVEIVSRADAPKRSYEDLFGSIKQR 333
           Q S+ K+VE+V+   AP    E L   I  +
Sbjct: 307 QLSFSKIVEVVAETTAPLTPIEKLLEKIPSK 329

BLAST of CmoCh03G013820 vs. ExPASy Swiss-Prot
Match: Q8SKU2 (Protein TIC 62, chloroplastic OS=Pisum sativum OX=3888 GN=TIC62 PE=1 SV=2)

HSP 1 Score: 115.9 bits (289), Expect = 8.5e-25
Identity = 98/290 (33.79%), Postives = 146/290 (50.34%), Query Frame = 0

Query: 65  STPTSSIAYRQMEG---NEITEEVAEK--QSEDNLNVKKKIFVAGASGSTGKRIVEQLLA 124
           S+ +SS    Q  G   +   E + EK    +DNL     +FVAGA+G  G R V +L+ 
Sbjct: 56  SSSSSSSIRAQASGSTKSSTAEGIPEKTDSKDDNL-----VFVAGATGKVGSRTVRELIK 115

Query: 125 RGFAVKAGVRDVSKA----------KTTLSPGNPDLQIVKADVTEGSAKLAEAIGS---D 184
            GF V+AGVR+  KA          K   + G  +  + K ++ E   + A+ IGS   +
Sbjct: 116 LGFKVRAGVRNAQKAGALVQSVKQLKLDGASGGGE-AVEKLEIVECDLEKADQIGSALGN 175

Query: 185 SEAVICATGF--RRGWDLFAPWKVDNFGTVNLVQACRELGINRFILISSILVNGAAMGQI 244
           +  VICA G   +  +D+  P ++D   T NLV A     +N FIL++S+  N   +   
Sbjct: 176 ASTVICAIGASEKEIFDITGPCRIDYRATKNLVDAATVAKVNHFILVTSLGTNKFGL--- 235

Query: 245 LNPAYIFLNVLGLTLIAKLQAEQHIRKSGINYTIIRPGGLKNE----PPTGNLVMTAEDT 304
             PA I LN+    LI K +AE+ +  SGI YTI+RPGG++        T N+ ++ EDT
Sbjct: 236 --PAAI-LNLFWGVLIWKRKAEEALLASGIPYTIVRPGGMERPTDAYKETHNVTLSTEDT 295

Query: 305 LYEGSISRDLVAEVAVEALVHSQASY-KVVEIVSRADAPKRSYEDLFGSI 330
           L+ G +S   VAE+      +   SY K+VE+++   AP    E L   I
Sbjct: 296 LFGGQVSNLQVAELMAIMAKNPDLSYCKIVEVIAETTAPLTPAEKLLTRI 333

BLAST of CmoCh03G013820 vs. ExPASy Swiss-Prot
Match: Q10A77 (Protein TIC 62, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=TIC62 PE=1 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 6.8e-22
Identity = 82/254 (32.28%), Postives = 130/254 (51.18%), Query Frame = 0

Query: 100 IFVAGASGSTGKRIVEQLLARGFAVKAGVRDVSKAKTTL-------------SPGNPDLQ 159
           +F+AGA+G  G R V + +  GF V+AGVR   +A + +             SP    L+
Sbjct: 75  VFIAGATGKVGSRAVREFIKLGFRVRAGVRSAQRASSLVQSVEQLKVDDDATSPAE-RLE 134

Query: 160 IVKADV-TEGSAKLAEAIGSDSEAVICATGFRRG--WDLFAPWKVDNFGTVNLVQACREL 219
           IV+ D+  +  + +  AIG ++  V+C+ G       D+  P+++D   T NLVQA    
Sbjct: 135 IVECDLEKQAQSDIVSAIG-NAAIVVCSIGASEKDILDVTGPYRIDYMATNNLVQAATAA 194

Query: 220 GINRFILISSILVNGAAMGQILNPAYIFLNVLGLTLIAKLQAEQHIRKSGINYTIIRPGG 279
            +  FIL++S+  N     +I  PA++ LN+    L  K +AE+ +  SG+ YTI+RPGG
Sbjct: 195 KVEHFILVTSLGTN-----RIGFPAFL-LNLFWGVLCWKRRAEEALIGSGLPYTIVRPGG 254

Query: 280 LKNE----PPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSQASY-KVVEIVSRADAP 333
           ++        T NLV+  EDT   G +S   VAE+      + + +Y KVVE ++   AP
Sbjct: 255 MERPTDAFKETHNLVVAVEDTYVGGLVSNLQVAELIACIASNRRTAYCKVVEAIAETTAP 314

BLAST of CmoCh03G013820 vs. ExPASy Swiss-Prot
Match: O07609 (Uncharacterized sugar epimerase YhfK OS=Bacillus subtilis (strain 168) OX=224308 GN=yhfK PE=2 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 4.0e-14
Identity = 65/222 (29.28%), Postives = 102/222 (45.95%), Query Frame = 0

Query: 99  KIFVAGASGSTGKRIVEQLLAR-GFAVKAGVRDVSKAKTTLSPGNPDLQIVKADVTEGSA 158
           K+F+ GA+G  G+R+V         +++A VR   + K +L     +  +      EGS 
Sbjct: 2   KVFLIGANGQIGQRLVSLFQDNPDHSIRAMVRK-EEQKASLEAAGAEAVLAN---LEGSP 61

Query: 159 KLAEAIGSDSEAVICATGFRRGWDLFAPWKVDNFGTVNLVQACRELGINRFILISSILV- 218
           +   A     +A+I   G            VD  G    ++A    GI RFI++S++   
Sbjct: 62  EEIAAAAKGCDAIIFTAGSGGSTGYDKTLLVDLDGAAKAIEAAAIAGIKRFIMVSALQAH 121

Query: 219 NGAAMGQILNPAYIFLNVLGLTLIAKLQAEQHIRKSGINYTIIRPGGLKNEPPTGNLVMT 278
           N     + L P Y          +AK  A++ +  SG+ YTIIRPGGL+NEP TG   ++
Sbjct: 122 NRENWNEALKPYY----------VAKHYADKILEASGLTYTIIRPGGLRNEPGTG--TVS 181

Query: 279 AEDTLYEGSISRDLVAEVAVEALVHSQASYKVVEIVSRADAP 319
           A   L  G ISRD VA+  + +L       +  ++ +  D P
Sbjct: 182 AAKDLERGFISRDDVAKTVIASLDEKNTENRAFDL-TEGDTP 206

BLAST of CmoCh03G013820 vs. ExPASy TrEMBL
Match: A0A6J1INH1 (uncharacterized protein At2g34460, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111478024 PE=4 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 1.6e-135
Identity = 256/258 (99.22%), Postives = 257/258 (99.61%), Query Frame = 0

Query: 75  QMEGNEITEEVAEKQSEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFAVKAGVRDVSKA 134
           +MEGNEITEEVAEKQSEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFAVKAGVRDVSKA
Sbjct: 50  KMEGNEITEEVAEKQSEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFAVKAGVRDVSKA 109

Query: 135 KTTLSPGNPDLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRRGWDLFAPWKVDNFGTV 194
           KTTLSPGNPDLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRRGWDLFAPWKVDNFGTV
Sbjct: 110 KTTLSPGNPDLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRRGWDLFAPWKVDNFGTV 169

Query: 195 NLVQACRELGINRFILISSILVNGAAMGQILNPAYIFLNVLGLTLIAKLQAEQHIRKSGI 254
           NLVQACRELGINRFILISSILVNGAAMGQILNPAYIFLNVLGLTLIAKLQAEQHIRKSGI
Sbjct: 170 NLVQACRELGINRFILISSILVNGAAMGQILNPAYIFLNVLGLTLIAKLQAEQHIRKSGI 229

Query: 255 NYTIIRPGGLKNEPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSQASYKVVEIVSR 314
           NYTIIRPGGLKNEPPTGNLVM AEDTLYEGSISRDLVAEVAVEALVHSQASYKVVEIVSR
Sbjct: 230 NYTIIRPGGLKNEPPTGNLVMAAEDTLYEGSISRDLVAEVAVEALVHSQASYKVVEIVSR 289

Query: 315 ADAPKRSYEDLFGSIKQR 333
           ADAPKRSYEDLFGSIKQR
Sbjct: 290 ADAPKRSYEDLFGSIKQR 307

BLAST of CmoCh03G013820 vs. ExPASy TrEMBL
Match: A0A6J1EDM3 (uncharacterized protein At2g34460, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111433212 PE=4 SV=1)

HSP 1 Score: 491.9 bits (1265), Expect = 2.1e-135
Identity = 261/275 (94.91%), Postives = 266/275 (96.73%), Query Frame = 0

Query: 58  SSPPSPFSTPTSSIAYRQMEGNEITEEVAEKQSEDNLNVKKKIFVAGASGSTGKRIVEQL 117
           SS    F++ +   +Y+ MEGNEITEEVAEKQSEDNLNVKKKIFVAGASGSTGKRIVEQL
Sbjct: 35  SSSSKKFASISILNSYK-MEGNEITEEVAEKQSEDNLNVKKKIFVAGASGSTGKRIVEQL 94

Query: 118 LARGFAVKAGVRDVSKAKTTLSPGNPDLQIVKADVTEGSAKLAEAIGSDSEAVICATGFR 177
           LARGFAVKAGVRDVSKAKTTLSPGNPDLQIVKADVTEGSAKLAEAIGSDSEAVICATGFR
Sbjct: 95  LARGFAVKAGVRDVSKAKTTLSPGNPDLQIVKADVTEGSAKLAEAIGSDSEAVICATGFR 154

Query: 178 RGWDLFAPWKVDNFGTVNLVQACRELGINRFILISSILVNGAAMGQILNPAYIFLNVLGL 237
           RGWDLFAPWKVDNFGTVNLVQACRELGINRFILISSILVNGAAMGQILNPAYIFLNVLGL
Sbjct: 155 RGWDLFAPWKVDNFGTVNLVQACRELGINRFILISSILVNGAAMGQILNPAYIFLNVLGL 214

Query: 238 TLIAKLQAEQHIRKSGINYTIIRPGGLKNEPPTGNLVMTAEDTLYEGSISRDLVAEVAVE 297
           TLIAKLQAEQHIRKSGINYTIIRPGGLKNEPPTGNLVMTAEDTLYEGSISRDLVAEVAVE
Sbjct: 215 TLIAKLQAEQHIRKSGINYTIIRPGGLKNEPPTGNLVMTAEDTLYEGSISRDLVAEVAVE 274

Query: 298 ALVHSQASYKVVEIVSRADAPKRSYEDLFGSIKQR 333
           ALVHSQASYKVVEIVSRADAPKRSYEDLFGSIKQR
Sbjct: 275 ALVHSQASYKVVEIVSRADAPKRSYEDLFGSIKQR 308

BLAST of CmoCh03G013820 vs. ExPASy TrEMBL
Match: A0A6J1D008 (uncharacterized protein At2g34460, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111015805 PE=4 SV=1)

HSP 1 Score: 476.5 bits (1225), Expect = 9.2e-131
Identity = 247/268 (92.16%), Postives = 258/268 (96.27%), Query Frame = 0

Query: 65  STPTSSIAYRQMEGNEITEEVAEKQSEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFAV 124
           S   S +   +MEG+EITEEVAEKQ+EDNLNVKKKIFVAGASGSTGKRIVEQLLARGF V
Sbjct: 42  SPSLSILNSNKMEGSEITEEVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFEV 101

Query: 125 KAGVRDVSKAKTTLSPGNPDLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRRGWDLFA 184
           KAGVRDVSKAKTTLSPGNPDLQIVKADVTEGSAKLAEAIGSDSEAVICATGFR GWDLFA
Sbjct: 102 KAGVRDVSKAKTTLSPGNPDLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRPGWDLFA 161

Query: 185 PWKVDNFGTVNLVQACRELGINRFILISSILVNGAAMGQILNPAYIFLNVLGLTLIAKLQ 244
           PWKVDN+GTVNLV+ACR+LGINRFILISSILVNGAAMGQILNPAYIFLNV GLTL+AKLQ
Sbjct: 162 PWKVDNYGTVNLVEACRQLGINRFILISSILVNGAAMGQILNPAYIFLNVFGLTLMAKLQ 221

Query: 245 AEQHIRKSGINYTIIRPGGLKNEPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSQA 304
           AEQHIRKSGINYTIIRPGGLKN+PPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHS+A
Sbjct: 222 AEQHIRKSGINYTIIRPGGLKNDPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEA 281

Query: 305 SYKVVEIVSRADAPKRSYEDLFGSIKQR 333
           SYKVVEIVSRADAPKR+YEDLFGSIKQR
Sbjct: 282 SYKVVEIVSRADAPKRTYEDLFGSIKQR 309

BLAST of CmoCh03G013820 vs. ExPASy TrEMBL
Match: A0A5D3CMY6 (DNA helicase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008920 PE=3 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 4.4e-125
Identity = 236/257 (91.83%), Postives = 246/257 (95.72%), Query Frame = 0

Query: 75   QMEGNEITEEVAEKQSEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFAVKAGVRDVSKA 134
            +MEGNEITEEVA+ QSE+N NVK+KIFVAGASGSTGK+IVEQLLARGFAVKAGVRDVSKA
Sbjct: 878  KMEGNEITEEVAQTQSEENSNVKRKIFVAGASGSTGKKIVEQLLARGFAVKAGVRDVSKA 937

Query: 135  KTTLSPGNPDLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRRGWDLFAPWKVDNFGTV 194
            KTTLSP NP LQIV+ADVTEGSAKLAEAIGSDSEAVICATGFRRGWDLFAPWKVDN GTV
Sbjct: 938  KTTLSPANPALQIVRADVTEGSAKLAEAIGSDSEAVICATGFRRGWDLFAPWKVDNLGTV 997

Query: 195  NLVQACRELGINRFILISSILVNGAAMGQILNPAYIFLNVLGLTLIAKLQAEQHIRKSGI 254
            NLV+ACR LGINRFILISSILVNGAAMGQILNPAYI LN LGL LIAKLQAE+HIR SGI
Sbjct: 998  NLVEACRGLGINRFILISSILVNGAAMGQILNPAYIILNALGLVLIAKLQAEKHIRNSGI 1057

Query: 255  NYTIIRPGGLKNEPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSQASYKVVEIVSR 314
            NYTIIRPGGLKNEPPTGNLVMT EDTLYEGSISRDLVAEVAVEAL+HSQASYKVVEIVSR
Sbjct: 1058 NYTIIRPGGLKNEPPTGNLVMTPEDTLYEGSISRDLVAEVAVEALLHSQASYKVVEIVSR 1117

Query: 315  ADAPKRSYEDLFGSIKQ 332
            ADAPKRSYEDLFGS+KQ
Sbjct: 1118 ADAPKRSYEDLFGSVKQ 1134

BLAST of CmoCh03G013820 vs. ExPASy TrEMBL
Match: A0A5A7U9X6 (NAD(P)-binding Rossmann-fold superfamily protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G001550 PE=4 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 4.4e-125
Identity = 236/257 (91.83%), Postives = 246/257 (95.72%), Query Frame = 0

Query: 75  QMEGNEITEEVAEKQSEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFAVKAGVRDVSKA 134
           +MEGNEITEEVA+ QSE+N NVK+KIFVAGASGSTGK+IVEQLLARGFAVKAGVRDVSKA
Sbjct: 51  KMEGNEITEEVAQTQSEENSNVKRKIFVAGASGSTGKKIVEQLLARGFAVKAGVRDVSKA 110

Query: 135 KTTLSPGNPDLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRRGWDLFAPWKVDNFGTV 194
           KTTLSP NP LQIV+ADVTEGSAKLAEAIGSDSEAVICATGFRRGWDLFAPWKVDN GTV
Sbjct: 111 KTTLSPANPALQIVRADVTEGSAKLAEAIGSDSEAVICATGFRRGWDLFAPWKVDNLGTV 170

Query: 195 NLVQACRELGINRFILISSILVNGAAMGQILNPAYIFLNVLGLTLIAKLQAEQHIRKSGI 254
           NLV+ACR LGINRFILISSILVNGAAMGQILNPAYI LN LGL LIAKLQAE+HIR SGI
Sbjct: 171 NLVEACRGLGINRFILISSILVNGAAMGQILNPAYIILNALGLVLIAKLQAEKHIRNSGI 230

Query: 255 NYTIIRPGGLKNEPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSQASYKVVEIVSR 314
           NYTIIRPGGLKNEPPTGNLVMT EDTLYEGSISRDLVAEVAVEAL+HSQASYKVVEIVSR
Sbjct: 231 NYTIIRPGGLKNEPPTGNLVMTPEDTLYEGSISRDLVAEVAVEALLHSQASYKVVEIVSR 290

Query: 315 ADAPKRSYEDLFGSIKQ 332
           ADAPKRSYEDLFGS+KQ
Sbjct: 291 ADAPKRSYEDLFGSVKQ 307

BLAST of CmoCh03G013820 vs. NCBI nr
Match: XP_022977850.1 (uncharacterized protein At2g34460, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 492.3 bits (1266), Expect = 3.3e-135
Identity = 256/258 (99.22%), Postives = 257/258 (99.61%), Query Frame = 0

Query: 75  QMEGNEITEEVAEKQSEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFAVKAGVRDVSKA 134
           +MEGNEITEEVAEKQSEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFAVKAGVRDVSKA
Sbjct: 50  KMEGNEITEEVAEKQSEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFAVKAGVRDVSKA 109

Query: 135 KTTLSPGNPDLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRRGWDLFAPWKVDNFGTV 194
           KTTLSPGNPDLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRRGWDLFAPWKVDNFGTV
Sbjct: 110 KTTLSPGNPDLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRRGWDLFAPWKVDNFGTV 169

Query: 195 NLVQACRELGINRFILISSILVNGAAMGQILNPAYIFLNVLGLTLIAKLQAEQHIRKSGI 254
           NLVQACRELGINRFILISSILVNGAAMGQILNPAYIFLNVLGLTLIAKLQAEQHIRKSGI
Sbjct: 170 NLVQACRELGINRFILISSILVNGAAMGQILNPAYIFLNVLGLTLIAKLQAEQHIRKSGI 229

Query: 255 NYTIIRPGGLKNEPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSQASYKVVEIVSR 314
           NYTIIRPGGLKNEPPTGNLVM AEDTLYEGSISRDLVAEVAVEALVHSQASYKVVEIVSR
Sbjct: 230 NYTIIRPGGLKNEPPTGNLVMAAEDTLYEGSISRDLVAEVAVEALVHSQASYKVVEIVSR 289

Query: 315 ADAPKRSYEDLFGSIKQR 333
           ADAPKRSYEDLFGSIKQR
Sbjct: 290 ADAPKRSYEDLFGSIKQR 307

BLAST of CmoCh03G013820 vs. NCBI nr
Match: XP_022925949.1 (uncharacterized protein At2g34460, chloroplastic [Cucurbita moschata] >KAG6581421.1 putative protein, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034702.1 putative protein, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 491.9 bits (1265), Expect = 4.4e-135
Identity = 261/275 (94.91%), Postives = 266/275 (96.73%), Query Frame = 0

Query: 58  SSPPSPFSTPTSSIAYRQMEGNEITEEVAEKQSEDNLNVKKKIFVAGASGSTGKRIVEQL 117
           SS    F++ +   +Y+ MEGNEITEEVAEKQSEDNLNVKKKIFVAGASGSTGKRIVEQL
Sbjct: 35  SSSSKKFASISILNSYK-MEGNEITEEVAEKQSEDNLNVKKKIFVAGASGSTGKRIVEQL 94

Query: 118 LARGFAVKAGVRDVSKAKTTLSPGNPDLQIVKADVTEGSAKLAEAIGSDSEAVICATGFR 177
           LARGFAVKAGVRDVSKAKTTLSPGNPDLQIVKADVTEGSAKLAEAIGSDSEAVICATGFR
Sbjct: 95  LARGFAVKAGVRDVSKAKTTLSPGNPDLQIVKADVTEGSAKLAEAIGSDSEAVICATGFR 154

Query: 178 RGWDLFAPWKVDNFGTVNLVQACRELGINRFILISSILVNGAAMGQILNPAYIFLNVLGL 237
           RGWDLFAPWKVDNFGTVNLVQACRELGINRFILISSILVNGAAMGQILNPAYIFLNVLGL
Sbjct: 155 RGWDLFAPWKVDNFGTVNLVQACRELGINRFILISSILVNGAAMGQILNPAYIFLNVLGL 214

Query: 238 TLIAKLQAEQHIRKSGINYTIIRPGGLKNEPPTGNLVMTAEDTLYEGSISRDLVAEVAVE 297
           TLIAKLQAEQHIRKSGINYTIIRPGGLKNEPPTGNLVMTAEDTLYEGSISRDLVAEVAVE
Sbjct: 215 TLIAKLQAEQHIRKSGINYTIIRPGGLKNEPPTGNLVMTAEDTLYEGSISRDLVAEVAVE 274

Query: 298 ALVHSQASYKVVEIVSRADAPKRSYEDLFGSIKQR 333
           ALVHSQASYKVVEIVSRADAPKRSYEDLFGSIKQR
Sbjct: 275 ALVHSQASYKVVEIVSRADAPKRSYEDLFGSIKQR 308

BLAST of CmoCh03G013820 vs. NCBI nr
Match: XP_023544344.1 (uncharacterized protein At2g34460, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 489.2 bits (1258), Expect = 2.8e-134
Identity = 259/275 (94.18%), Postives = 266/275 (96.73%), Query Frame = 0

Query: 58  SSPPSPFSTPTSSIAYRQMEGNEITEEVAEKQSEDNLNVKKKIFVAGASGSTGKRIVEQL 117
           SS    F++ +   +Y+ MEGNEITEEVAEKQSEDNLNVKKKIFVAGASGSTGKRIVEQL
Sbjct: 35  SSSSKKFASISILNSYK-MEGNEITEEVAEKQSEDNLNVKKKIFVAGASGSTGKRIVEQL 94

Query: 118 LARGFAVKAGVRDVSKAKTTLSPGNPDLQIVKADVTEGSAKLAEAIGSDSEAVICATGFR 177
           LARGFAVKAGVRDVSKAKTTLSPGNPDLQIV+ADVTEGSAKLAEAIGSDSEAVICATGFR
Sbjct: 95  LARGFAVKAGVRDVSKAKTTLSPGNPDLQIVRADVTEGSAKLAEAIGSDSEAVICATGFR 154

Query: 178 RGWDLFAPWKVDNFGTVNLVQACRELGINRFILISSILVNGAAMGQILNPAYIFLNVLGL 237
           RGWDLFAPWKVDNFGTVNLVQACRELGINRFILISSILVNGAAMGQILNPAYIFLNVLGL
Sbjct: 155 RGWDLFAPWKVDNFGTVNLVQACRELGINRFILISSILVNGAAMGQILNPAYIFLNVLGL 214

Query: 238 TLIAKLQAEQHIRKSGINYTIIRPGGLKNEPPTGNLVMTAEDTLYEGSISRDLVAEVAVE 297
           TLIAKLQAE+HIRKSGINYTIIRPGGLKNEPPTGNLVMTAEDTLYEGSISRDLVAEVAVE
Sbjct: 215 TLIAKLQAEKHIRKSGINYTIIRPGGLKNEPPTGNLVMTAEDTLYEGSISRDLVAEVAVE 274

Query: 298 ALVHSQASYKVVEIVSRADAPKRSYEDLFGSIKQR 333
           ALVHSQASYKVVEIVSRADAPKRSYEDLFGSIKQR
Sbjct: 275 ALVHSQASYKVVEIVSRADAPKRSYEDLFGSIKQR 308

BLAST of CmoCh03G013820 vs. NCBI nr
Match: XP_022146656.1 (uncharacterized protein At2g34460, chloroplastic [Momordica charantia])

HSP 1 Score: 476.5 bits (1225), Expect = 1.9e-130
Identity = 247/268 (92.16%), Postives = 258/268 (96.27%), Query Frame = 0

Query: 65  STPTSSIAYRQMEGNEITEEVAEKQSEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFAV 124
           S   S +   +MEG+EITEEVAEKQ+EDNLNVKKKIFVAGASGSTGKRIVEQLLARGF V
Sbjct: 42  SPSLSILNSNKMEGSEITEEVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFEV 101

Query: 125 KAGVRDVSKAKTTLSPGNPDLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRRGWDLFA 184
           KAGVRDVSKAKTTLSPGNPDLQIVKADVTEGSAKLAEAIGSDSEAVICATGFR GWDLFA
Sbjct: 102 KAGVRDVSKAKTTLSPGNPDLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRPGWDLFA 161

Query: 185 PWKVDNFGTVNLVQACRELGINRFILISSILVNGAAMGQILNPAYIFLNVLGLTLIAKLQ 244
           PWKVDN+GTVNLV+ACR+LGINRFILISSILVNGAAMGQILNPAYIFLNV GLTL+AKLQ
Sbjct: 162 PWKVDNYGTVNLVEACRQLGINRFILISSILVNGAAMGQILNPAYIFLNVFGLTLMAKLQ 221

Query: 245 AEQHIRKSGINYTIIRPGGLKNEPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSQA 304
           AEQHIRKSGINYTIIRPGGLKN+PPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHS+A
Sbjct: 222 AEQHIRKSGINYTIIRPGGLKNDPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEA 281

Query: 305 SYKVVEIVSRADAPKRSYEDLFGSIKQR 333
           SYKVVEIVSRADAPKR+YEDLFGSIKQR
Sbjct: 282 SYKVVEIVSRADAPKRTYEDLFGSIKQR 309

BLAST of CmoCh03G013820 vs. NCBI nr
Match: XP_038881265.1 (uncharacterized protein At2g34460, chloroplastic [Benincasa hispida])

HSP 1 Score: 471.1 bits (1211), Expect = 8.0e-129
Identity = 255/285 (89.47%), Postives = 265/285 (92.98%), Query Frame = 0

Query: 52  FPPPPRSSPPSPFST---PTSSI-AYRQMEGNEITEEVAEKQSEDNLNVKKKIFVAGASG 111
           F P  +S  P  FS+   P+ SI +  +MEGNEITEEVAEKQ EDNLN+K+KIFVAGA+G
Sbjct: 20  FCPLCKSFLPVSFSSNRFPSISILSSTKMEGNEITEEVAEKQIEDNLNMKRKIFVAGATG 79

Query: 112 STGKRIVEQLLARGFAVKAGVRDVSKAKTTLSPGNPDLQIVKADVTEGSAKLAEAIGSDS 171
           STGKRIVEQLLARGFAVKAGVRDVSKAKTTLSPGN  LQIVKADVTEGSAKLAEAIGSDS
Sbjct: 80  STGKRIVEQLLARGFAVKAGVRDVSKAKTTLSPGNSALQIVKADVTEGSAKLAEAIGSDS 139

Query: 172 EAVICATGFRRGWDLFAPWKVDNFGTVNLVQACRELGINRFILISSILVNGAAMGQILNP 231
           EAVICATGFR GWDLFAPWKVDNFGTVNLV+ACRELGINRFILISSILVNGAAMGQILNP
Sbjct: 140 EAVICATGFRPGWDLFAPWKVDNFGTVNLVEACRELGINRFILISSILVNGAAMGQILNP 199

Query: 232 AYIFLNVLGLTLIAKLQAEQHIRKSGINYTIIRPGGLKNEPPTGNLVMTAEDTLYEGSIS 291
           AYIFLNVLGLTLIAKLQAEQHIRKSGINYTIIRPGGLKNEPPTGNLVMT EDTLYEGSIS
Sbjct: 200 AYIFLNVLGLTLIAKLQAEQHIRKSGINYTIIRPGGLKNEPPTGNLVMTPEDTLYEGSIS 259

Query: 292 RDLVAEVAVEALVHSQASYKVVEIVSRADAPKRSYEDLFGSIKQR 333
           RDLV EVAVEALVHSQASYKVVEIVSRADAPKRSY+DLFGSIKQR
Sbjct: 260 RDLVGEVAVEALVHSQASYKVVEIVSRADAPKRSYDDLFGSIKQR 304

BLAST of CmoCh03G013820 vs. TAIR 10
Match: AT2G34460.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 365.9 bits (938), Expect = 3.4e-101
Identity = 186/262 (70.99%), Postives = 219/262 (83.59%), Query Frame = 0

Query: 69  SSIAYRQMEGNEITEEVAEKQSEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFAVKAGV 128
           SS   +       T    EK   +N    KK+FVAGA+G TGKRIVEQLL+RGFAVKAGV
Sbjct: 18  SSFFTKNKSFRSFTSIKMEKGEAENAVKTKKVFVAGATGQTGKRIVEQLLSRGFAVKAGV 77

Query: 129 RDVSKAKTTLSPGNPDLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRRGWDLFAPWKV 188
           RDV KAKT+    +P LQIV+ADVTEG  KLAE IG DS+AVICATGFR G+D+F PWKV
Sbjct: 78  RDVEKAKTSFK-DDPSLQIVRADVTEGPDKLAEVIGDDSQAVICATGFRPGFDIFTPWKV 137

Query: 189 DNFGTVNLVQACRELGINRFILISSILVNGAAMGQILNPAYIFLNVLGLTLIAKLQAEQH 248
           DNFGTVNLV ACR+ G+ +F+L+SSILVNGAAMGQILNPAY+FLN+ GLTL+AKLQAE++
Sbjct: 138 DNFGTVNLVDACRKQGVEKFVLVSSILVNGAAMGQILNPAYLFLNLFGLTLVAKLQAEKY 197

Query: 249 IRKSGINYTIIRPGGLKNEPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSQASYKV 308
           I+KSGINYTI+RPGGLKN+PPTGN+VM  EDTLYEGSISRDLVAEVAVEAL+  ++S+KV
Sbjct: 198 IKKSGINYTIVRPGGLKNDPPTGNVVMEPEDTLYEGSISRDLVAEVAVEALLQEESSFKV 257

Query: 309 VEIVSRADAPKRSYEDLFGSIK 331
           VEIV+RA+APKRSY+DLF S+K
Sbjct: 258 VEIVARAEAPKRSYKDLFASVK 278

BLAST of CmoCh03G013820 vs. TAIR 10
Match: AT3G18890.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 115.9 bits (289), Expect = 6.1e-26
Identity = 92/271 (33.95%), Postives = 138/271 (50.92%), Query Frame = 0

Query: 85  VAEKQSEDNLNVKKK--IFVAGASGSTGKRIVEQLLARGFAVKAGVRDVSKAKTTLSP-- 144
           V  + S  NLN K++  +FVAGA+G  G R V +LL  GF V+AGVR   +A + +    
Sbjct: 67  VVTEASPTNLNSKEEDLVFVAGATGKVGSRTVRELLKLGFRVRAGVRSAQRAGSLVQSVK 126

Query: 145 ----GNPD--------LQIVKADVTEGSAKLAEAIGSDSEAVICATGF--RRGWDLFAPW 204
                N D        L+IV+ D+ E    +  A+G+ S  +IC  G   +   D+  P+
Sbjct: 127 EMKLQNTDEGTQPVEKLEIVECDL-EKKDSIQPALGNAS-VIICCIGASEKEISDITGPY 186

Query: 205 KVDNFGTVNLVQACRELGINRFILISSILVNGAAMGQILNPAYIFLNVLGLTLIAKLQAE 264
           ++D   T NLV A     +N FIL++S+  N         PA I LN+    L  K +AE
Sbjct: 187 RIDYLATKNLVDAATSAKVNNFILVTSLGTNKFGF-----PAAI-LNLFWGVLCWKRKAE 246

Query: 265 QHIRKSGINYTIIRPGGLKNE----PPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHS 324
           + + +SG+NY I+RPGG++        T NL +  +DTL+ G +S   VAE+      + 
Sbjct: 247 EALIESGLNYAIVRPGGMERPTDAYKETHNLTLALDDTLFGGQVSNLQVAELLACMAKNP 306

Query: 325 QASY-KVVEIVSRADAPKRSYEDLFGSIKQR 333
           Q S+ K+VE+V+   AP    E L   I  +
Sbjct: 307 QLSFSKIVEVVAETTAPLTPIEKLLEKIPSK 329

BLAST of CmoCh03G013820 vs. TAIR 10
Match: AT2G37660.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 68.9 bits (167), Expect = 8.5e-12
Identity = 69/255 (27.06%), Postives = 122/255 (47.84%), Query Frame = 0

Query: 100 IFVAGASGSTGKRIVEQLLARGFAVKAGVRDVSKAKTTLSPGNPDLQIVKADVTEGSAKL 159
           + V GA G TG+ + ++L  R     A  R + + K +    N + ++   D+ + +A +
Sbjct: 79  VLVTGAGGRTGQIVYKKLKERSEQFVA--RGLVRTKESKEKINGEDEVFIGDIRD-TASI 138

Query: 160 AEAIGSDSEAVICATG---FRRGWDLFA-------------PWKVDNFGTVNLVQACREL 219
           A A+      VI  +     + G+D                P +VD  G  N + A +  
Sbjct: 139 APAVEGIDALVILTSAVPQMKPGFDPSKGGRPEFFFDDGAYPEQVDWIGQKNQIDAAKAA 198

Query: 220 GINRFILISSILVNGAAMGQILNPAYIFLNVLGLT--LIAKLQAEQHIRKSGINYTIIRP 279
           G+ + +L+      G+  G  +N     LN +G    L+ K +AEQ++  SGI YTIIR 
Sbjct: 199 GVKQIVLV------GSMGGTNINHP---LNSIGNANILVWKRKAEQYLADSGIPYTIIRA 258

Query: 280 GGLKN-EPPTGNLVMTAEDTLYE---GSISRDLVAEVAVEALVHSQASYKVVEIVSRAD- 330
           GGL++ +     L++  +D L E    +I+R  VAEV V+AL   +A +K +++ S+ + 
Sbjct: 259 GGLQDKDGGIRELLVGKDDELLETETRTIARADVAEVCVQALQLEEAKFKALDLASKPEG 318

BLAST of CmoCh03G013820 vs. TAIR 10
Match: AT5G02240.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 62.4 bits (150), Expect = 8.0e-10
Identity = 75/260 (28.85%), Postives = 122/260 (46.92%), Query Frame = 0

Query: 100 IFVAGASGSTGKRIVEQLLARG---FAVKAGVRDVSKAKTTLSPGNPDLQIVKADVTEGS 159
           + V GASG TG +IV + L  G   F  K  VR  ++ K  +  G  D+ I   D+T+  
Sbjct: 7   VLVTGASGRTG-QIVYKKLKEGSDKFVAKGLVRS-AQGKEKIG-GEADVFI--GDITDAD 66

Query: 160 AKLAEAIGSDSEAVICAT--GFRRGWD---------LFA----PWKVDNFGTVNLVQACR 219
           +      G D+  ++ +     + G+D         +F     P +VD  G  N + A +
Sbjct: 67  SINPAFQGIDALVILTSAVPKMKPGFDPTKGGRPEFIFEDGQYPEQVDWIGQKNQIDAAK 126

Query: 220 ELGINRFILISSILVNGAAMGQILNPAYIFLNVL--GLTLIAKLQAEQHIRKSGINYTII 279
             G+   +++ S       MG   NP +  LN L  G  L+ K +AEQ++  SG  YTII
Sbjct: 127 VAGVKHIVVVGS-------MGG-TNPDHP-LNKLGNGNILVWKRKAEQYLADSGTPYTII 186

Query: 280 RPGG-LKNEPPTGNLVMTAEDTLYE---GSISRDLVAEVAVEALVHSQASYKVVEIVSRA 333
           R GG L  E     L++  +D L +    ++ R  VAEV ++AL+  +A  K  ++ S+ 
Sbjct: 187 RAGGLLDKEGGVRELLVGKDDELLQTDTKTVPRADVAEVCIQALLFEEAKNKAFDLGSKP 246

BLAST of CmoCh03G013820 vs. TAIR 10
Match: AT3G46780.1 (plastid transcriptionally active 16 )

HSP 1 Score: 62.0 bits (149), Expect = 1.0e-09
Identity = 65/256 (25.39%), Postives = 117/256 (45.70%), Query Frame = 0

Query: 100 IFVAGASGSTGKRIVEQLLARGFAVKAGVRDVSKAK-------TTLSPGNPDLQIVKA-- 159
           IFVAGA+G  G RI + LL RGF+V+AGV D+  A+       T     N +++ + A  
Sbjct: 94  IFVAGATGQAGIRIAQTLLQRGFSVRAGVPDLGAAQDLARVAATYKILSNDEVKRLNAVQ 153

Query: 160 DVTEGSAKLAEAIGSDSEAVICATGFRRGWDLFAPWKVDNFGTVNLVQACRELGINRFIL 219
              + +  +A+AIG+ ++ V+       G D     +V     + +VQA    G++   +
Sbjct: 154 SPFQDAESIAKAIGNATKVVVTVGATENGPDA----QVSTSDALLVVQAAELAGVSHVAI 213

Query: 220 ISSILVNGAAMGQILNPAYIFLNVLGLTL-IAKLQAEQHIRKSGINYTIIRPGGLKNEPP 279
           +    ++G+    +      F N+   +  +      + + ++ + YT+I+    ++  P
Sbjct: 214 VYDGTISGSTYNVLDGITSFFGNLFAKSQPLTISDLIEKVAQTDVAYTLIKTSLTEDFSP 273

Query: 280 --TGNLVMTAE-DTLYEGSISRD-----------LVAEVAVEALVHSQASYKVVEIVSRA 332
               N+V++AE      GS S +           LVA++     V   A  KVVE+ +  
Sbjct: 274 EKAYNVVVSAEGSNSGSGSSSSEAYKVPKLKIASLVADIFANTAV---AENKVVEVSTDP 333

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8H1244.7e-10070.99Uncharacterized protein At2g34460, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Q8H0U58.5e-2533.95Protein TIC 62, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC62 PE=1 SV=1[more]
Q8SKU28.5e-2533.79Protein TIC 62, chloroplastic OS=Pisum sativum OX=3888 GN=TIC62 PE=1 SV=2[more]
Q10A776.8e-2232.28Protein TIC 62, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=TIC62 ... [more]
O076094.0e-1429.28Uncharacterized sugar epimerase YhfK OS=Bacillus subtilis (strain 168) OX=224308... [more]
Match NameE-valueIdentityDescription
A0A6J1INH11.6e-13599.22uncharacterized protein At2g34460, chloroplastic-like OS=Cucurbita maxima OX=366... [more]
A0A6J1EDM32.1e-13594.91uncharacterized protein At2g34460, chloroplastic OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1D0089.2e-13192.16uncharacterized protein At2g34460, chloroplastic OS=Momordica charantia OX=3673 ... [more]
A0A5D3CMY64.4e-12591.83DNA helicase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008920 ... [more]
A0A5A7U9X64.4e-12591.83NAD(P)-binding Rossmann-fold superfamily protein OS=Cucumis melo var. makuwa OX=... [more]
Match NameE-valueIdentityDescription
XP_022977850.13.3e-13599.22uncharacterized protein At2g34460, chloroplastic-like [Cucurbita maxima][more]
XP_022925949.14.4e-13594.91uncharacterized protein At2g34460, chloroplastic [Cucurbita moschata] >KAG658142... [more]
XP_023544344.12.8e-13494.18uncharacterized protein At2g34460, chloroplastic-like [Cucurbita pepo subsp. pep... [more]
XP_022146656.11.9e-13092.16uncharacterized protein At2g34460, chloroplastic [Momordica charantia][more]
XP_038881265.18.0e-12989.47uncharacterized protein At2g34460, chloroplastic [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT2G34460.13.4e-10170.99NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G18890.16.1e-2633.95NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G37660.18.5e-1227.06NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G02240.18.0e-1028.85NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G46780.11.0e-0925.39plastid transcriptionally active 16 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016040NAD(P)-binding domainPFAMPF13460NAD_binding_10coord: 104..301
e-value: 1.9E-44
score: 151.7
NoneNo IPR availableGENE3D3.40.50.720coord: 81..321
e-value: 4.2E-49
score: 169.1
NoneNo IPR availablePANTHERPTHR15020FLAVIN REDUCTASE-RELATEDcoord: 76..332
NoneNo IPR availablePANTHERPTHR15020:SF39SUBFAMILY NOT NAMEDcoord: 76..332
NoneNo IPR availableCDDcd05243SDR_a5coord: 99..314
e-value: 7.89732E-54
score: 173.576
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 98..313

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh03G013820.1CmoCh03G013820.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007049 cell cycle
biological_process GO:0032508 DNA duplex unwinding
biological_process GO:0006270 DNA replication initiation
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0042555 MCM complex
cellular_component GO:0000347 THO complex
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0003677 DNA binding
molecular_function GO:0003678 DNA helicase activity