CmoCh03G013560 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh03G013560
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	Cmo_Chr03: 10016424 .. 10017813 (-)
RNA-Seq Expression	CmoCh03G013560
Synteny	CmoCh03G013560

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGAAGATTGGAAAGATGTGTGTGTAAATCTACTTCTTTTTGTTTGAGTGTGTGTATAAATAGATGATAAACTAATGCCATAAAACGCAGCCACAAACAAGAAGACAAGATGATAAGCTTAAAAGAGCTGGTAGAAAGCAGCTCCAAAATCAATATCCCACAAAACTTCATTCGTTTGGATCAGGAGCCTTCTTCTACCTCCCATCCTTCCACGTTCCCCGCCCCACCGACCATCGATATGAGTCGATTACTCTCACCGCAACATTCCCGCTCTGAGCTTCTCAATCTTCACTCCGCTTGCAAGCAATGGGGCTTGTTTCAGGTACCTACTACTATGATCCATGATATGATATGATATGTGTCTGACTAAGTCAAACGTGCGTTGTTGTTGTTGTTTATAACTTTTTTCAGCTAGTGAACCATGGGGTTAGCAGTTCATTGTTGGAAGAGGTGAAGAGTGAGATTGAAGGGTTCTTTGAGCTTCCACTAGATGAGAAGATGAAGTTTTGTATGAAGGCCGGAGAATTTGAGGGCTATGGAACCGTGGTTCGATCCATGGATCAGAAGCTTGATTGGGGCGATAGGTTTTACATGATCACCAATCCTCTTTCGAGAAGAAAACCCCATCTCCTTCCCCACTTCCCTCCATCCTTAAGGTCCCTCCCTAACACGAACCTACCGGGATTATCAAAATATGTCAAAACAAATAAATGAATTTTTCTCAAATTGAAGTGAATAACTACCAAACAATTTTTTATCTTTTATTAAATCAAGCTTATTAATTTTACGTGAAAATACAGAAATACGTTAGAATCGTACTTGAAAGCGACAAAGAAGATAGCGATGAGTTTGTTGAGGATGATGGCGGGGAATTTGGAGATGGAAGTGAGGAAGATGGAGGAGTTGTTCGAGGATGGAATGGAGGCAGTAAGGATGACGTATTATCCGCCATGTCCAAGGCCGGAGTTAGTGGTGGGTTTGAGACCGCACTCGGACGCGACGGGGGTGACGATATTGAACCAGCTGAACGGAGTGGAAGGTCTCCAAGTGAAGAAAGATGGGACATGGTTCCCAGTGAGTTTCATCCCAGACGGGCTGATGGTGAACGTGGGCGACGTTGTTGAGATCATGAGCAATGGGGTTTACAACAGCATTGAGCACAGGGCGGCGGTGAACTCGGAGAAAGGGAGAATGTCTATAGCGGTGTTCTACAATCCCAGATTTGAGGCAGAGATCGGACCACAAACTCAAAGCCCACCATTGTTTAGAAGGATTGTGATGGAGGATTATTTCAAGGACTTCTTCACCCAATCTTTCAGTGGAAAATCCCACTTGGAGCGGATGAAGATCCCACCAACTAATCAACCCAATTAATCCTCATTCATCTTTC

mRNA sequence

AAGAAGATTGGAAAGATGTGTGTGTAAATCTACTTCTTTTTGTTTGAGTGTGTGTATAAATAGATGATAAACTAATGCCATAAAACGCAGCCACAAACAAGAAGACAAGATGATAAGCTTAAAAGAGCTGGTAGAAAGCAGCTCCAAAATCAATATCCCACAAAACTTCATTCGTTTGGATCAGGAGCCTTCTTCTACCTCCCATCCTTCCACGTTCCCCGCCCCACCGACCATCGATATGAGTCGATTACTCTCACCGCAACATTCCCGCTCTGAGCTTCTCAATCTTCACTCCGCTTGCAAGCAATGGGGCTTGTTTCAGCTAGTGAACCATGGGGTTAGCAGTTCATTGTTGGAAGAGGTGAAGAGTGAGATTGAAGGGTTCTTTGAGCTTCCACTAGATGAGAAGATGAAGTTTTGTATGAAGGCCGGAGAATTTGAGGGCTATGGAACCGTGGTTCGATCCATGGATCAGAAGCTTGATTGGGGCGATAGGTTTTACATGATCACCAATCCTCTTTCGAGAAGAAAACCCCATCTCCTTCCCCACTTCCCTCCATCCTTAAGAAATACGTTAGAATCGTACTTGAAAGCGACAAAGAAGATAGCGATGAGTTTGTTGAGGATGATGGCGGGGAATTTGGAGATGGAAGTGAGGAAGATGGAGGAGTTGTTCGAGGATGGAATGGAGGCAGTAAGGATGACGTATTATCCGCCATGTCCAAGGCCGGAGTTAGTGGTGGGTTTGAGACCGCACTCGGACGCGACGGGGGTGACGATATTGAACCAGCTGAACGGAGTGGAAGGTCTCCAAGTGAAGAAAGATGGGACATGGTTCCCAGTGAGTTTCATCCCAGACGGGCTGATGGTGAACGTGGGCGACGTTGTTGAGATCATGAGCAATGGGGTTTACAACAGCATTGAGCACAGGGCGGCGGTGAACTCGGAGAAAGGGAGAATGTCTATAGCGGTGTTCTACAATCCCAGATTTGAGGCAGAGATCGGACCACAAACTCAAAGCCCACCATTGTTTAGAAGGATTGTGATGGAGGATTATTTCAAGGACTTCTTCACCCAATCTTTCAGTGGAAAATCCCACTTGGAGCGGATGAAGATCCCACCAACTAATCAACCCAATTAATCCTCATTCATCTTTC

Coding sequence (CDS)

ATGATAAGCTTAAAAGAGCTGGTAGAAAGCAGCTCCAAAATCAATATCCCACAAAACTTCATTCGTTTGGATCAGGAGCCTTCTTCTACCTCCCATCCTTCCACGTTCCCCGCCCCACCGACCATCGATATGAGTCGATTACTCTCACCGCAACATTCCCGCTCTGAGCTTCTCAATCTTCACTCCGCTTGCAAGCAATGGGGCTTGTTTCAGCTAGTGAACCATGGGGTTAGCAGTTCATTGTTGGAAGAGGTGAAGAGTGAGATTGAAGGGTTCTTTGAGCTTCCACTAGATGAGAAGATGAAGTTTTGTATGAAGGCCGGAGAATTTGAGGGCTATGGAACCGTGGTTCGATCCATGGATCAGAAGCTTGATTGGGGCGATAGGTTTTACATGATCACCAATCCTCTTTCGAGAAGAAAACCCCATCTCCTTCCCCACTTCCCTCCATCCTTAAGAAATACGTTAGAATCGTACTTGAAAGCGACAAAGAAGATAGCGATGAGTTTGTTGAGGATGATGGCGGGGAATTTGGAGATGGAAGTGAGGAAGATGGAGGAGTTGTTCGAGGATGGAATGGAGGCAGTAAGGATGACGTATTATCCGCCATGTCCAAGGCCGGAGTTAGTGGTGGGTTTGAGACCGCACTCGGACGCGACGGGGGTGACGATATTGAACCAGCTGAACGGAGTGGAAGGTCTCCAAGTGAAGAAAGATGGGACATGGTTCCCAGTGAGTTTCATCCCAGACGGGCTGATGGTGAACGTGGGCGACGTTGTTGAGATCATGAGCAATGGGGTTTACAACAGCATTGAGCACAGGGCGGCGGTGAACTCGGAGAAAGGGAGAATGTCTATAGCGGTGTTCTACAATCCCAGATTTGAGGCAGAGATCGGACCACAAACTCAAAGCCCACCATTGTTTAGAAGGATTGTGATGGAGGATTATTTCAAGGACTTCTTCACCCAATCTTTCAGTGGAAAATCCCACTTGGAGCGGATGAAGATCCCACCAACTAATCAACCCAATTAA

Protein sequence

MISLKELVESSSKINIPQNFIRLDQEPSSTSHPSTFPAPPTIDMSRLLSPQHSRSELLNLHSACKQWGLFQLVNHGVSSSLLEEVKSEIEGFFELPLDEKMKFCMKAGEFEGYGTVVRSMDQKLDWGDRFYMITNPLSRRKPHLLPHFPPSLRNTLESYLKATKKIAMSLLRMMAGNLEMEVRKMEELFEDGMEAVRMTYYPPCPRPELVVGLRPHSDATGVTILNQLNGVEGLQVKKDGTWFPVSFIPDGLMVNVGDVVEIMSNGVYNSIEHRAAVNSEKGRMSIAVFYNPRFEAEIGPQTQSPPLFRRIVMEDYFKDFFTQSFSGKSHLERMKIPPTNQPN

Homology

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BLAST of CmoCh03G013560 vs. ExPASy Swiss-Prot
Match: D4N502 (Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 9.0e-86
Identity = 154/306 (50.33%), Postives = 215/306 (70.26%), Query Frame = 0


Query: 40  PTIDMSRLLSPQH--SRSELLNLHSACKQWGLFQLVNHGVSSSLLEEVKSEIEGFFELPL 99
           P ID+  LLSP+    + EL  LHSACK+WG FQLVNHGV + L++ +KSEI+GFF LP+
Sbjct: 55  PVIDLQNLLSPEPVVGKLELDKLHSACKEWGFFQLVNHGVDALLMDNIKSEIKGFFNLPM 114

Query: 100 DEKMKFCMKAGEFEGYG-TVVRSMDQKLDWGDRFYMITNPLSRRKPHLLPHFPPSLRNTL 159
           +EK K+  + G+FEG+G   + S DQ+LDW + F M++ PL  RKPHL P  P   R TL
Sbjct: 115 NEKTKYGQQDGDFEGFGQPYIESEDQRLDWTEVFSMLSLPLHLRKPHLFPELPLPFRETL 174

Query: 160 ESYLKATKKIAMSLLRMMAGNLEM-EVRKMEELFEDGMEAVRMTYYPPCPRPELVVGLRP 219
           ESYL   KK++  +  M+  +L++ E++ M +LFEDG++ +RM YYPPCPRPELV+GL  
Sbjct: 175 ESYLSKMKKLSTVVFEMLEKSLQLVEIKGMTDLFEDGLQTMRMNYYPPCPRPELVLGLTS 234

Query: 220 HSDATGVTILNQLNGVEGLQVKKDGTWFPVSFIPDGLMVNVGDVVEIMSNGVYNSIEHRA 279
           HSD +G+TIL QLN VEGLQ++K+  W  +  +PD  +VNVGD++EIM+NG+Y S+EHRA
Sbjct: 235 HSDFSGLTILLQLNEVEGLQIRKEERWISIKPLPDAFIVNVGDILEIMTNGIYRSVEHRA 294

Query: 280 AVNSEKGRMSIAVFYNPRFEAEIGP-----QTQSPPLFRRIVMEDYFKDFFTQSFSGKSH 337
            VNS K R+SIA F++ + E+EIGP       ++P LF+R   ED  K+  ++   GKS 
Sbjct: 295 VVNSTKERLSIATFHDSKLESEIGPISSLVTPETPALFKRGRYEDILKENLSRKLDGKSF 354

[Close]

BLAST of CmoCh03G013560 vs. ExPASy Swiss-Prot
Match: Q39224 (Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 2.2e-84
Identity = 155/343 (45.19%), Postives = 222/343 (64.72%), Query Frame = 0


Query: 3   SLKELVESSSKINIPQNFIRLDQEPSSTSHPSTFPAP-PTIDMSRLLSPQHSRSELLNLH 62
           S++E+V+  +   +P  ++R DQ+ +            P IDM RL S     SE+  L 
Sbjct: 16  SVQEMVKEKTITTVPPRYVRSDQDKTEVDDDFDVKIEIPIIDMKRLCSSTTMDSEVEKLD 75

Query: 63  SACKQWGLFQLVNHGVSSSLLEEVKSEIEGFFELPLDEKMKFCMKAGEFEGYG-TVVRSM 122
            ACK+WG FQLVNHG+ SS L++VKSEI+ FF LP++EK KF  +  E EG+G   V S 
Sbjct: 76  FACKEWGFFQLVNHGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGFGQAFVVSE 135

Query: 123 DQKLDWGDRFYMITNPLSRRKPHLLPHFPPSLRNTLESYLKATKKIAMSLLRMMAGNLEM 182
           DQKLDW D F+    P+  RKPHL P  P   R+TLE Y    + +A  L+  MA  LE+
Sbjct: 136 DQKLDWADLFFHTVQPVELRKPHLFPKLPLPFRDTLEMYSSEVQSVAKILIAKMARALEI 195

Query: 183 EVRKMEELFE--DGMEAVRMTYYPPCPRPELVVGLRPHSDATGVTILNQLNGVEGLQVKK 242
           +  ++E+LF+  D ++++RM YYPPCP+P+ V+GL PHSD+ G+T+L Q+N VEGLQ+KK
Sbjct: 196 KPEELEKLFDDVDSVQSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQVNDVEGLQIKK 255

Query: 243 DGTWFPVSFIPDGLMVNVGDVVEIMSNGVYNSIEHRAAVNSEKGRMSIAVFYNPRFEAEI 302
           DG W PV  +P+  +VN+GDV+EI++NG Y SIEHR  VNSEK R+SIA F+N     E+
Sbjct: 256 DGKWVPVKPLPNAFIVNIGDVLEIITNGTYRSIEHRGVVNSEKERLSIATFHNVGMYKEV 315

Query: 303 GP-----QTQSPPLFRRIVMEDYFKDFFTQSFSGKSHLERMKI 337
           GP     + Q    F+R+ M++Y    F+++  GK++L+ ++I
Sbjct: 316 GPAKSLVERQKVARFKRLTMKEYNDGLFSRTLDGKAYLDALRI 358

[Close]

BLAST of CmoCh03G013560 vs. ExPASy Swiss-Prot
Match: D4N501 (Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum OX=3469 GN=DIOX2 PE=2 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 2.1e-79
Identity = 150/309 (48.54%), Postives = 207/309 (66.99%), Query Frame = 0


Query: 40  PTIDMSRLLS--PQHSRSELLNLHSACKQWGLFQLVNHGVSSSLLEEVKSEIEGFFELPL 99
           P ID+  L+S  P   + EL  LHSACK+WG FQ+VNHGV +SL++ VKS+I+GFF L +
Sbjct: 56  PVIDIENLISSEPVTEKLELDRLHSACKEWGFFQVVNHGVDTSLVDNVKSDIQGFFNLSM 115

Query: 100 DEKMKFCMKAGEFEGYG-TVVRSMDQKLDWGDRFYMITNPLSRRKPHLLPHFPPSLRNTL 159
           +EK+K+  K G+ EG+G   V S DQ LDW D F ++T PL  RKPHL    P  LR T+
Sbjct: 116 NEKIKYGQKDGDVEGFGQAFVASEDQTLDWADIFMILTLPLHLRKPHLFSKLPLPLRETI 175

Query: 160 ESYLKATKKIAMSLLRMMAGNLE---MEVRKMEELFEDGMEAVRMTYYPPCPRPELVVGL 219
           ESY    KK++M L   M   L+   +E++++ E+F+D  + +RM YYPPCP+PEL +GL
Sbjct: 176 ESYSSEMKKLSMVLFEKMEKALQVQAVEIKEISEVFKDMTQVMRMNYYPPCPQPELAIGL 235

Query: 220 RPHSDATGVTILNQLNGVEGLQVKKDGTWFPVSFIPDGLMVNVGDVVEIMSNGVYNSIEH 279
            PHSD  G+TIL QLN VEGLQ+K +G W  V  +P+  +VNVGDV+EIM+NG+Y S++H
Sbjct: 236 TPHSDFGGLTILLQLNEVEGLQIKNEGRWISVKPLPNAFVVNVGDVLEIMTNGMYRSVDH 295

Query: 280 RAAVNSEKGRMSIAVFYNPRFEAEIGP-----QTQSPPLFRR-IVMEDYFKDFFTQSFSG 337
           RA VNS K R+SIA F++P  E+EIGP        +P LFR      +  ++F ++   G
Sbjct: 296 RAVVNSTKERLSIATFHDPNLESEIGPISSLITPNTPALFRSGSTYGELVEEFHSRKLDG 355

[Close]

BLAST of CmoCh03G013560 vs. ExPASy Swiss-Prot
Match: D4N500 (Thebaine 6-O-demethylase OS=Papaver somniferum OX=3469 GN=T6ODM PE=1 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 1.8e-78
Identity = 154/336 (45.83%), Postives = 213/336 (63.39%), Query Frame = 0


Query: 16  IPQNFIRLDQE---PSSTSHPSTFPAPPTIDMSRLLSPQH--SRSELLNLHSACKQWGLF 75
           IP  ++  ++    P   S  +     P ID+  LLSP+    + EL  LH ACK+WG F
Sbjct: 29  IPSRYVCANENLLLPMGASVINDHETIPVIDIENLLSPEPIIGKLELDRLHFACKEWGFF 88

Query: 76  QLVNHGVSSSLLEEVKSEIEGFFELPLDEKMKFCMKAGEFEGYGT-VVRSMDQKLDWGDR 135
           Q+VNHGV +SL++ VKSEI+GFF L +DEK K+  + G+ EG+G   + S DQ LDW D 
Sbjct: 89  QVVNHGVDASLVDSVKSEIQGFFNLSMDEKTKYEQEDGDVEGFGQGFIESEDQTLDWADI 148

Query: 136 FYMITNPLSRRKPHLLPHFPPSLRNTLESYLKATKKIAMSLLRMMAGNLEM---EVRKME 195
           F M T PL  RKPHL    P  LR T+ESY    KK++M L   M   L++   E++ M 
Sbjct: 149 FMMFTLPLHLRKPHLFSKLPVPLRETIESYSSEMKKLSMVLFNKMEKALQVQAAEIKGMS 208

Query: 196 ELFEDGMEAVRMTYYPPCPRPELVVGLRPHSDATGVTILNQLNGVEGLQVKKDGTWFPVS 255
           E+F DG +A+RM YYPPCP+P L +GL  HSD  G+TIL Q+N VEGLQ+K++GTW  V 
Sbjct: 209 EVFIDGTQAMRMNYYPPCPQPNLAIGLTSHSDFGGLTILLQINEVEGLQIKREGTWISVK 268

Query: 256 FIPDGLMVNVGDVVEIMSNGVYNSIEHRAAVNSEKGRMSIAVFYNPRFEAEIGP-----Q 315
            +P+  +VNVGD++EIM+NG+Y+S++HRA VNS   R+SIA F++P  E+ IGP      
Sbjct: 269 PLPNAFVVNVGDILEIMTNGIYHSVDHRAVVNSTNERLSIATFHDPSLESVIGPISSLIT 328

Query: 316 TQSPPLFRR-IVMEDYFKDFFTQSFSGKSHLERMKI 337
            ++P LF+      D  ++  T+   GKS L+ M+I
Sbjct: 329 PETPALFKSGSTYGDLVEECKTRKLDGKSFLDSMRI 364

[Close]

BLAST of CmoCh03G013560 vs. ExPASy Swiss-Prot
Match: Q94LP4 (2-oxoglutarate-dependent dioxygenase 11 OS=Oryza sativa subsp. japonica OX=39947 GN=2ODD11 PE=1 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 8.7e-65
Identity = 130/330 (39.39%), Postives = 188/330 (56.97%), Query Frame = 0


Query: 15  NIPQNFIRLDQEPSSTSHPSTFP---APPTIDMSRLLSPQHSRSELLNLHSACKQWGLFQ 74
           +IP+ +IR   E SS    + +    A P ID+ +LL PQ S  E + L SAC+ WG F 
Sbjct: 28  HIPERYIR--PEASSEEVINNYQGDMAIPIIDLKKLLCPQSSEEECVKLRSACQYWGFFL 87

Query: 75  LVNHGVSSSLLEEVKSEIEGFFELPLDEKMKFCMKAGEFEGYG-TVVRSMDQKLDWGDRF 134
           L+NHGV   ++  +K +I  FF  PLD K ++       EGYG + V S DQKLDW D  
Sbjct: 88  LINHGVPDEVIANLKRDIVDFFSQPLDTKKEYTQLPNSLEGYGQSFVFSEDQKLDWADML 147

Query: 135 YMITNPLSRRKPHLLPHFPPSLRNTLESYLKATKKIAMSLLRMMAGNLEMEVRKMEELFE 194
           Y+  +P   R     P  P S R ++++Y   TK +A+ L   MA  +  +   + +LFE
Sbjct: 148 YLHVHPSDSRDLRFWPTSPASFRQSIDAYSSETKSLALCLFEFMAKAVGAKPESLLDLFE 207

Query: 195 DGMEAVRMTYYPPCPRPELVVGLRPHSDATGVTILNQLNGVEGLQVKKDGTWFPVSFIPD 254
           +    +RM YYPPC + + V+GL PHSDA G+T+L ++N V+GLQ+KKDG WF +     
Sbjct: 208 EQPRGLRMAYYPPCRQADKVMGLSPHSDAGGLTLLLEINNVQGLQIKKDGKWFSIDAPNG 267

Query: 255 GLMVNVGDVVEIMSNGVYNSIEHRAAVNSEKGRMSIAVFYNPRFEAEIGPQTQ----SPP 314
            L+ N+GD +EI+SNG + S+EHRA +N  K R+S A+F+ P     I P  +       
Sbjct: 268 ALIANIGDTLEILSNGKFRSVEHRAVINPNKERISAALFHYPSENMVISPLPEFVKDGKV 327

Query: 315 LFRRIVMEDYFKDFFTQSFSGKSHLERMKI 337
            +R I   D+ K  FTQ   GK+ +E +K+
Sbjct: 328 KYRSISYLDFMKQIFTQQLDGKNRVEVLKL 355

[Close]

BLAST of CmoCh03G013560 vs. ExPASy TrEMBL
Match: A0A6J1ED48 (codeine O-demethylase-like OS=Cucurbita moschata OX=3662 GN=LOC111433087 PE=3 SV=1)

HSP 1 Score: 696.0 bits (1795), Expect = 7.6e-197
Identity = 343/343 (100.00%), Postives = 343/343 (100.00%), Query Frame = 0


Query: 1   MISLKELVESSSKINIPQNFIRLDQEPSSTSHPSTFPAPPTIDMSRLLSPQHSRSELLNL 60
           MISLKELVESSSKINIPQNFIRLDQEPSSTSHPSTFPAPPTIDMSRLLSPQHSRSELLNL
Sbjct: 1   MISLKELVESSSKINIPQNFIRLDQEPSSTSHPSTFPAPPTIDMSRLLSPQHSRSELLNL 60

Query: 61  HSACKQWGLFQLVNHGVSSSLLEEVKSEIEGFFELPLDEKMKFCMKAGEFEGYGTVVRSM 120
           HSACKQWGLFQLVNHGVSSSLLEEVKSEIEGFFELPLDEKMKFCMKAGEFEGYGTVVRSM
Sbjct: 61  HSACKQWGLFQLVNHGVSSSLLEEVKSEIEGFFELPLDEKMKFCMKAGEFEGYGTVVRSM 120

Query: 121 DQKLDWGDRFYMITNPLSRRKPHLLPHFPPSLRNTLESYLKATKKIAMSLLRMMAGNLEM 180
           DQKLDWGDRFYMITNPLSRRKPHLLPHFPPSLRNTLESYLKATKKIAMSLLRMMAGNLEM
Sbjct: 121 DQKLDWGDRFYMITNPLSRRKPHLLPHFPPSLRNTLESYLKATKKIAMSLLRMMAGNLEM 180

Query: 181 EVRKMEELFEDGMEAVRMTYYPPCPRPELVVGLRPHSDATGVTILNQLNGVEGLQVKKDG 240
           EVRKMEELFEDGMEAVRMTYYPPCPRPELVVGLRPHSDATGVTILNQLNGVEGLQVKKDG
Sbjct: 181 EVRKMEELFEDGMEAVRMTYYPPCPRPELVVGLRPHSDATGVTILNQLNGVEGLQVKKDG 240

Query: 241 TWFPVSFIPDGLMVNVGDVVEIMSNGVYNSIEHRAAVNSEKGRMSIAVFYNPRFEAEIGP 300
           TWFPVSFIPDGLMVNVGDVVEIMSNGVYNSIEHRAAVNSEKGRMSIAVFYNPRFEAEIGP
Sbjct: 241 TWFPVSFIPDGLMVNVGDVVEIMSNGVYNSIEHRAAVNSEKGRMSIAVFYNPRFEAEIGP 300

Query: 301 QTQSPPLFRRIVMEDYFKDFFTQSFSGKSHLERMKIPPTNQPN 344
           QTQSPPLFRRIVMEDYFKDFFTQSFSGKSHLERMKIPPTNQPN
Sbjct: 301 QTQSPPLFRRIVMEDYFKDFFTQSFSGKSHLERMKIPPTNQPN 343

[Close]

BLAST of CmoCh03G013560 vs. ExPASy TrEMBL
Match: A0A6J1INU8 (codeine O-demethylase-like OS=Cucurbita maxima OX=3661 GN=LOC111478599 PE=3 SV=1)

HSP 1 Score: 677.9 bits (1748), Expect = 2.2e-191
Identity = 332/343 (96.79%), Postives = 339/343 (98.83%), Query Frame = 0


Query: 1   MISLKELVESSSKINIPQNFIRLDQEPSSTSHPSTFPAPPTIDMSRLLSPQHSRSELLNL 60
           MISLKELVESSSKI+IPQNFIRLDQEPSSTSHPSTFP PPTIDMSRLLSPQHSRSELLNL
Sbjct: 1   MISLKELVESSSKISIPQNFIRLDQEPSSTSHPSTFPVPPTIDMSRLLSPQHSRSELLNL 60

Query: 61  HSACKQWGLFQLVNHGVSSSLLEEVKSEIEGFFELPLDEKMKFCMKAGEFEGYGTVVRSM 120
           HSACKQWGLFQLVNHGVS SLLEEVKSEIEGFFELPLDEKMKFCMKAGEFEGYGTVVRSM
Sbjct: 61  HSACKQWGLFQLVNHGVSCSLLEEVKSEIEGFFELPLDEKMKFCMKAGEFEGYGTVVRSM 120

Query: 121 DQKLDWGDRFYMITNPLSRRKPHLLPHFPPSLRNTLESYLKATKKIAMSLLRMMAGNLEM 180
           DQKLDWGDRFYMIT+PLSRRKPHLLPHFP SLRNTLESYLK TKKIAM+LLRMMAGNLEM
Sbjct: 121 DQKLDWGDRFYMITHPLSRRKPHLLPHFPSSLRNTLESYLKETKKIAMTLLRMMAGNLEM 180

Query: 181 EVRKMEELFEDGMEAVRMTYYPPCPRPELVVGLRPHSDATGVTILNQLNGVEGLQVKKDG 240
           EVRKMEELFEDGMEAVRMTYYPPCPRPELVVGLRPHSDATG+TILNQLNGVEGLQVKKDG
Sbjct: 181 EVRKMEELFEDGMEAVRMTYYPPCPRPELVVGLRPHSDATGLTILNQLNGVEGLQVKKDG 240

Query: 241 TWFPVSFIPDGLMVNVGDVVEIMSNGVYNSIEHRAAVNSEKGRMSIAVFYNPRFEAEIGP 300
           TWFPVSFIPDGLMVNVGDV+EIMSNGVYNSIEHRAAVNSEKGRMSIAVFYNPRFEAEIGP
Sbjct: 241 TWFPVSFIPDGLMVNVGDVLEIMSNGVYNSIEHRAAVNSEKGRMSIAVFYNPRFEAEIGP 300

Query: 301 QTQSPPLFRRIVMEDYFKDFFTQSFSGKSHLERMKIPPTNQPN 344
           QTQ+PPLFRRIVMEDYFKDFFTQSF+GKSHLERMKIPPTNQPN
Sbjct: 301 QTQNPPLFRRIVMEDYFKDFFTQSFNGKSHLERMKIPPTNQPN 343

[Close]

BLAST of CmoCh03G013560 vs. ExPASy TrEMBL
Match: A0A5A7UA06 (Codeine O-demethylase-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G001880 PE=3 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 4.8e-159
Identity = 279/346 (80.64%), Postives = 307/346 (88.73%), Query Frame = 0


Query: 1   MISLKELVESSSKINIPQNFIRLDQEPSSTSHPSTFPAPPTIDMSRLLSPQHSRSELLNL 60
           MIS+KELVE SSK+ IPQNF+RLDQE SSTS PSTFP PP IDMSRLLSPQHSRSELLNL
Sbjct: 1   MISVKELVEKSSKLTIPQNFLRLDQEASSTSDPSTFPTPPIIDMSRLLSPQHSRSELLNL 60

Query: 61  HSACKQWGLFQLVNHGVSSSLLEEVKSEIEGFFELPLDEKMKFCMKAGEFEGYGTVVRSM 120
           HSAC QWGLFQLVNHGVS SLL E+K EIEGFFELPL EKMK+ M++GE EGYGTVVRSM
Sbjct: 61  HSACIQWGLFQLVNHGVSLSLLGELKHEIEGFFELPLKEKMKYGMRSGEVEGYGTVVRSM 120

Query: 121 DQKLDWGDRFYMITNPLSRRKPHLLPHFPPSLRNTLESYLKATKKIAMSLLRMMAGNLEM 180
           +QKLDWGDR Y+ITNPLSRRKPHLLP  P  LRN LESYLK T+KIAM+L  M+AGNL +
Sbjct: 121 EQKLDWGDRVYIITNPLSRRKPHLLPSLPLYLRNALESYLKETRKIAMTLFGMIAGNLNI 180

Query: 181 EVRKMEELFEDGMEAVRMTYYPPCPRPELVVGLRPHSDATGVTILNQLNGVEGLQVKKDG 240
           EVRK+EELFEDGMEA+RM+YYPPCP PELVVGLRPHSDA+G+TILNQLNGVEGLQVKKDG
Sbjct: 181 EVRKLEELFEDGMEAIRMSYYPPCPTPELVVGLRPHSDASGLTILNQLNGVEGLQVKKDG 240

Query: 241 TWFPVSFIPDGLMVNVGDVVEIMSNGVYNSIEHRAAVNSEKGRMSIAVFYNPRFEAEIGP 300
            WFPVSFIPD  +VNVGD++EI+SNG+YNSIEHRA VNSEK RMSIAVFYNPRF+ EI P
Sbjct: 241 IWFPVSFIPDAFIVNVGDIIEIISNGLYNSIEHRATVNSEKERMSIAVFYNPRFDGEIAP 300

Query: 301 QTQS---PPLFRRIVMEDYFKDFFTQSFSGKSHLERMKIPPTNQPN 344
            T S   PPLF+ I+MEDYFKDFFTQSF+GKSHL+RMKIP T QPN
Sbjct: 301 FTASQLNPPLFKNIIMEDYFKDFFTQSFNGKSHLDRMKIPTTQQPN 346

[Close]

BLAST of CmoCh03G013560 vs. ExPASy TrEMBL
Match: A0A6J1CKJ4 (protein SRG1-like OS=Momordica charantia OX=3673 GN=LOC111012449 PE=3 SV=1)

HSP 1 Score: 523.9 bits (1348), Expect = 5.2e-145
Identity = 259/342 (75.73%), Postives = 293/342 (85.67%), Query Frame = 0


Query: 7   LVESSSKINIPQNFIRLDQEPSSTSHPSTFPAPPTIDMSRLLSPQHSRSELLNLHSACKQ 66
           + + SSK  IP+NFIRLDQEP S+S P +FP PPTIDM++LLS  HS SEL  LHSAC+Q
Sbjct: 1   MAKKSSK--IPENFIRLDQEPCSSSDPGSFPVPPTIDMNQLLS--HSESELQKLHSACQQ 60

Query: 67  WGLFQLVNHGVSSSLLEEVKSEIEGFFELPLDEKMKFCMKAGEFEGYGTVVRSMDQKLDW 126
           WGLFQLVNHGV   LLEEVK EIE FFE+PL+EKMKF +K GE EGYG++V S DQKLDW
Sbjct: 61  WGLFQLVNHGVRRCLLEEVKREIEEFFEVPLEEKMKFGIKGGEVEGYGSMVLSQDQKLDW 120

Query: 127 GDRFYMITNPLSRRKPHLLPHFPPSLRNTLESYLKATKKIAMSLLRMMAGNLEMEVRKME 186
           GDRFY+ITNP+SRR+PHLLP  P SLRNTLE YLK TKKIAM+LL+M+AG L M+VRKME
Sbjct: 121 GDRFYIITNPISRRRPHLLPRLPSSLRNTLELYLKETKKIAMALLKMVAGGLGMDVRKME 180

Query: 187 ELFEDGMEAVRMTYYPPCPRPELVVGLRPHSDATGVTILNQLNGVEGLQVKKDGTWFPVS 246
           ELFEDGMEA+RMTYYPPCP PELVVGLRPHSDATG+TILNQLNGVEGLQVKKDG WFPVS
Sbjct: 181 ELFEDGMEAMRMTYYPPCPTPELVVGLRPHSDATGLTILNQLNGVEGLQVKKDGVWFPVS 240

Query: 247 FIPDGLMVNVGDVVEIMSNGVYNSIEHRAAVNSEKGRMSIAVFYNPRFEAEIGPQT---- 306
           FIPD  +VNVGD++EI+SNG Y+SIEHRA VNSE GRMSIA FYNPRF+AEIGP+T    
Sbjct: 241 FIPDAFIVNVGDILEIVSNGCYSSIEHRAVVNSENGRMSIAAFYNPRFDAEIGPETCILS 300

Query: 307 -QSPPLFRRIVMEDYFKDFFTQSFSGKSHLERMKIPPTNQPN 344
            Q+PP FR I+MEDY KDFF+ SF+GKSHLERMKI PT+ PN
Sbjct: 301 PQNPPKFRTILMEDYVKDFFSHSFNGKSHLERMKI-PTHHPN 337

[Close]

BLAST of CmoCh03G013560 vs. ExPASy TrEMBL
Match: A0A1S3AYY1 (codeine O-demethylase-like OS=Cucumis melo OX=3656 GN=LOC103484411 PE=3 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 2.5e-139
Identity = 244/303 (80.53%), Postives = 270/303 (89.11%), Query Frame = 0


Query: 44  MSRLLSPQHSRSELLNLHSACKQWGLFQLVNHGVSSSLLEEVKSEIEGFFELPLDEKMKF 103
           MSRLLSPQHSRSELLNLHSAC QWGLFQLVNHGVS SLL  +K EIEGFFELPL+EKMK+
Sbjct: 1   MSRLLSPQHSRSELLNLHSACIQWGLFQLVNHGVSLSLLGGLKHEIEGFFELPLEEKMKY 60

Query: 104 CMKAGEFEGYGTVVRSMDQKLDWGDRFYMITNPLSRRKPHLLPHFPPSLRNTLESYLKAT 163
            M++GE EGYGTVVRSM+QKLDWGDR Y+ITNPLSRRKPHLLP  P  LRN LESYLK T
Sbjct: 61  GMRSGEVEGYGTVVRSMEQKLDWGDRVYIITNPLSRRKPHLLPSLPLYLRNALESYLKET 120

Query: 164 KKIAMSLLRMMAGNLEMEVRKMEELFEDGMEAVRMTYYPPCPRPELVVGLRPHSDATGVT 223
           +KIAM+L  M+AGNL +EVRK+EELFEDGMEA+RM+YYPPCP PELVVGLRPHSDA+G+T
Sbjct: 121 RKIAMTLFGMIAGNLNIEVRKLEELFEDGMEAIRMSYYPPCPTPELVVGLRPHSDASGLT 180

Query: 224 ILNQLNGVEGLQVKKDGTWFPVSFIPDGLMVNVGDVVEIMSNGVYNSIEHRAAVNSEKGR 283
           ILNQLNGVEGLQVKKDG WFPVSFIPD  +VNVGD++EI+SNG+YNSIEHRA VNSEK R
Sbjct: 181 ILNQLNGVEGLQVKKDGIWFPVSFIPDAFIVNVGDIIEIISNGLYNSIEHRATVNSEKER 240

Query: 284 MSIAVFYNPRFEAEIGPQTQS---PPLFRRIVMEDYFKDFFTQSFSGKSHLERMKIPPTN 343
           MSIAVFYNPRF+ EI P T S   PPLF+ I+MEDYFKDFFTQSF+GKSHL+RMKIP T 
Sbjct: 241 MSIAVFYNPRFDGEIAPFTASQLNPPLFKNIIMEDYFKDFFTQSFNGKSHLDRMKIPTTQ 300

[Close]

BLAST of CmoCh03G013560 vs. NCBI nr
Match: XP_022925781.1 (codeine O-demethylase-like [Cucurbita moschata] >KAG7034678.1 Codeine O-demethylase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 696.0 bits (1795), Expect = 1.6e-196
Identity = 343/343 (100.00%), Postives = 343/343 (100.00%), Query Frame = 0


Query: 1   MISLKELVESSSKINIPQNFIRLDQEPSSTSHPSTFPAPPTIDMSRLLSPQHSRSELLNL 60
           MISLKELVESSSKINIPQNFIRLDQEPSSTSHPSTFPAPPTIDMSRLLSPQHSRSELLNL
Sbjct: 1   MISLKELVESSSKINIPQNFIRLDQEPSSTSHPSTFPAPPTIDMSRLLSPQHSRSELLNL 60

Query: 61  HSACKQWGLFQLVNHGVSSSLLEEVKSEIEGFFELPLDEKMKFCMKAGEFEGYGTVVRSM 120
           HSACKQWGLFQLVNHGVSSSLLEEVKSEIEGFFELPLDEKMKFCMKAGEFEGYGTVVRSM
Sbjct: 61  HSACKQWGLFQLVNHGVSSSLLEEVKSEIEGFFELPLDEKMKFCMKAGEFEGYGTVVRSM 120

Query: 121 DQKLDWGDRFYMITNPLSRRKPHLLPHFPPSLRNTLESYLKATKKIAMSLLRMMAGNLEM 180
           DQKLDWGDRFYMITNPLSRRKPHLLPHFPPSLRNTLESYLKATKKIAMSLLRMMAGNLEM
Sbjct: 121 DQKLDWGDRFYMITNPLSRRKPHLLPHFPPSLRNTLESYLKATKKIAMSLLRMMAGNLEM 180

Query: 181 EVRKMEELFEDGMEAVRMTYYPPCPRPELVVGLRPHSDATGVTILNQLNGVEGLQVKKDG 240
           EVRKMEELFEDGMEAVRMTYYPPCPRPELVVGLRPHSDATGVTILNQLNGVEGLQVKKDG
Sbjct: 181 EVRKMEELFEDGMEAVRMTYYPPCPRPELVVGLRPHSDATGVTILNQLNGVEGLQVKKDG 240

Query: 241 TWFPVSFIPDGLMVNVGDVVEIMSNGVYNSIEHRAAVNSEKGRMSIAVFYNPRFEAEIGP 300
           TWFPVSFIPDGLMVNVGDVVEIMSNGVYNSIEHRAAVNSEKGRMSIAVFYNPRFEAEIGP
Sbjct: 241 TWFPVSFIPDGLMVNVGDVVEIMSNGVYNSIEHRAAVNSEKGRMSIAVFYNPRFEAEIGP 300

Query: 301 QTQSPPLFRRIVMEDYFKDFFTQSFSGKSHLERMKIPPTNQPN 344
           QTQSPPLFRRIVMEDYFKDFFTQSFSGKSHLERMKIPPTNQPN
Sbjct: 301 QTQSPPLFRRIVMEDYFKDFFTQSFSGKSHLERMKIPPTNQPN 343

[Close]

BLAST of CmoCh03G013560 vs. NCBI nr
Match: KAG6581392.1 (Protein SRG1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 688.0 bits (1774), Expect = 4.3e-194
Identity = 339/343 (98.83%), Postives = 340/343 (99.13%), Query Frame = 0


Query: 1   MISLKELVESSSKINIPQNFIRLDQEPSSTSHPSTFPAPPTIDMSRLLSPQHSRSELLNL 60
           MISLKELVESSSKINIPQNFIRLDQEPSSTSHPSTFP PPTIDMSRLLS QHSRSELLNL
Sbjct: 1   MISLKELVESSSKINIPQNFIRLDQEPSSTSHPSTFPVPPTIDMSRLLSLQHSRSELLNL 60

Query: 61  HSACKQWGLFQLVNHGVSSSLLEEVKSEIEGFFELPLDEKMKFCMKAGEFEGYGTVVRSM 120
           HSACKQWGLFQLVNHGVS SLLEEVKSEIEGFFELPLDEKMKFCMKAGEFEGYGTVVRSM
Sbjct: 61  HSACKQWGLFQLVNHGVSGSLLEEVKSEIEGFFELPLDEKMKFCMKAGEFEGYGTVVRSM 120

Query: 121 DQKLDWGDRFYMITNPLSRRKPHLLPHFPPSLRNTLESYLKATKKIAMSLLRMMAGNLEM 180
           DQKLDWGDRFYMITNPLSRRKPHLLPHFPPSLRNTLESYLKATKKIAM+LLRMMAGNLEM
Sbjct: 121 DQKLDWGDRFYMITNPLSRRKPHLLPHFPPSLRNTLESYLKATKKIAMTLLRMMAGNLEM 180

Query: 181 EVRKMEELFEDGMEAVRMTYYPPCPRPELVVGLRPHSDATGVTILNQLNGVEGLQVKKDG 240
           EVRKMEELFEDGMEAVRMTYYPPCPRPELVVGLRPHSDATGVTILNQLNGVEGLQVKKDG
Sbjct: 181 EVRKMEELFEDGMEAVRMTYYPPCPRPELVVGLRPHSDATGVTILNQLNGVEGLQVKKDG 240

Query: 241 TWFPVSFIPDGLMVNVGDVVEIMSNGVYNSIEHRAAVNSEKGRMSIAVFYNPRFEAEIGP 300
           TWFPVSFIPDGLMVNVGDVVEIMSNGVYNSIEHRAAVNSEKGRMSIAVFYNPRFEAEIGP
Sbjct: 241 TWFPVSFIPDGLMVNVGDVVEIMSNGVYNSIEHRAAVNSEKGRMSIAVFYNPRFEAEIGP 300

Query: 301 QTQSPPLFRRIVMEDYFKDFFTQSFSGKSHLERMKIPPTNQPN 344
           QTQSPPLFRRIVMEDYFKDFFTQSFSGKSHLERMKIPPTNQPN
Sbjct: 301 QTQSPPLFRRIVMEDYFKDFFTQSFSGKSHLERMKIPPTNQPN 343

[Close]

BLAST of CmoCh03G013560 vs. NCBI nr
Match: XP_022978731.1 (codeine O-demethylase-like [Cucurbita maxima])

HSP 1 Score: 677.9 bits (1748), Expect = 4.4e-191
Identity = 332/343 (96.79%), Postives = 339/343 (98.83%), Query Frame = 0


Query: 1   MISLKELVESSSKINIPQNFIRLDQEPSSTSHPSTFPAPPTIDMSRLLSPQHSRSELLNL 60
           MISLKELVESSSKI+IPQNFIRLDQEPSSTSHPSTFP PPTIDMSRLLSPQHSRSELLNL
Sbjct: 1   MISLKELVESSSKISIPQNFIRLDQEPSSTSHPSTFPVPPTIDMSRLLSPQHSRSELLNL 60

Query: 61  HSACKQWGLFQLVNHGVSSSLLEEVKSEIEGFFELPLDEKMKFCMKAGEFEGYGTVVRSM 120
           HSACKQWGLFQLVNHGVS SLLEEVKSEIEGFFELPLDEKMKFCMKAGEFEGYGTVVRSM
Sbjct: 61  HSACKQWGLFQLVNHGVSCSLLEEVKSEIEGFFELPLDEKMKFCMKAGEFEGYGTVVRSM 120

Query: 121 DQKLDWGDRFYMITNPLSRRKPHLLPHFPPSLRNTLESYLKATKKIAMSLLRMMAGNLEM 180
           DQKLDWGDRFYMIT+PLSRRKPHLLPHFP SLRNTLESYLK TKKIAM+LLRMMAGNLEM
Sbjct: 121 DQKLDWGDRFYMITHPLSRRKPHLLPHFPSSLRNTLESYLKETKKIAMTLLRMMAGNLEM 180

Query: 181 EVRKMEELFEDGMEAVRMTYYPPCPRPELVVGLRPHSDATGVTILNQLNGVEGLQVKKDG 240
           EVRKMEELFEDGMEAVRMTYYPPCPRPELVVGLRPHSDATG+TILNQLNGVEGLQVKKDG
Sbjct: 181 EVRKMEELFEDGMEAVRMTYYPPCPRPELVVGLRPHSDATGLTILNQLNGVEGLQVKKDG 240

Query: 241 TWFPVSFIPDGLMVNVGDVVEIMSNGVYNSIEHRAAVNSEKGRMSIAVFYNPRFEAEIGP 300
           TWFPVSFIPDGLMVNVGDV+EIMSNGVYNSIEHRAAVNSEKGRMSIAVFYNPRFEAEIGP
Sbjct: 241 TWFPVSFIPDGLMVNVGDVLEIMSNGVYNSIEHRAAVNSEKGRMSIAVFYNPRFEAEIGP 300

Query: 301 QTQSPPLFRRIVMEDYFKDFFTQSFSGKSHLERMKIPPTNQPN 344
           QTQ+PPLFRRIVMEDYFKDFFTQSF+GKSHLERMKIPPTNQPN
Sbjct: 301 QTQNPPLFRRIVMEDYFKDFFTQSFNGKSHLERMKIPPTNQPN 343

[Close]

BLAST of CmoCh03G013560 vs. NCBI nr
Match: XP_023544159.1 (codeine O-demethylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 676.4 bits (1744), Expect = 1.3e-190
Identity = 332/343 (96.79%), Postives = 337/343 (98.25%), Query Frame = 0


Query: 1   MISLKELVESSSKINIPQNFIRLDQEPSSTSHPSTFPAPPTIDMSRLLSPQHSRSELLNL 60
           MISLKELVESSSKINIPQNFIRLDQEPSSTSHPSTFP PPTIDMSRLLSPQHS SELLNL
Sbjct: 1   MISLKELVESSSKINIPQNFIRLDQEPSSTSHPSTFPVPPTIDMSRLLSPQHSCSELLNL 60

Query: 61  HSACKQWGLFQLVNHGVSSSLLEEVKSEIEGFFELPLDEKMKFCMKAGEFEGYGTVVRSM 120
           HSACKQWGLFQLVNHGVS SLLEEVKSEIEGFFELPLDEKMKFCMK GEFEGYGTVVRSM
Sbjct: 61  HSACKQWGLFQLVNHGVSCSLLEEVKSEIEGFFELPLDEKMKFCMKGGEFEGYGTVVRSM 120

Query: 121 DQKLDWGDRFYMITNPLSRRKPHLLPHFPPSLRNTLESYLKATKKIAMSLLRMMAGNLEM 180
           DQKLDWGDRFYMITNPLSRRKPHLLPHFPPSLRNTLESYLKATKKIAM+LLRMMAGNLEM
Sbjct: 121 DQKLDWGDRFYMITNPLSRRKPHLLPHFPPSLRNTLESYLKATKKIAMTLLRMMAGNLEM 180

Query: 181 EVRKMEELFEDGMEAVRMTYYPPCPRPELVVGLRPHSDATGVTILNQLNGVEGLQVKKDG 240
           EV KMEELFEDGMEAVRMTYYPPCPRPELVVGLRPHSDATG+TILNQLNGVEGLQVKKDG
Sbjct: 181 EVMKMEELFEDGMEAVRMTYYPPCPRPELVVGLRPHSDATGLTILNQLNGVEGLQVKKDG 240

Query: 241 TWFPVSFIPDGLMVNVGDVVEIMSNGVYNSIEHRAAVNSEKGRMSIAVFYNPRFEAEIGP 300
           TWFPVSFIP+GLMVNVGDVVEIMSNGVYNSIEHRAAVNSEKGRMSIAVFYNPRFEAEIGP
Sbjct: 241 TWFPVSFIPEGLMVNVGDVVEIMSNGVYNSIEHRAAVNSEKGRMSIAVFYNPRFEAEIGP 300

Query: 301 QTQSPPLFRRIVMEDYFKDFFTQSFSGKSHLERMKIPPTNQPN 344
           QTQ+PPLFRRIVMEDYFKDFFTQSF+GKSHLERMKI PTNQPN
Sbjct: 301 QTQTPPLFRRIVMEDYFKDFFTQSFNGKSHLERMKIAPTNQPN 343

[Close]

BLAST of CmoCh03G013560 vs. NCBI nr
Match: XP_038883393.1 (protein SRG1-like [Benincasa hispida])

HSP 1 Score: 578.2 bits (1489), Expect = 4.8e-161
Identity = 284/345 (82.32%), Postives = 313/345 (90.72%), Query Frame = 0


Query: 1   MISLKELVESSSKINIPQNFIRLDQEPSSTSHPSTFPAPPTIDMSRLLSPQHSRSELLNL 60
           MIS+KELVE SS I IPQNFIRLDQEPSSTS PSTFP PPTIDM+RLLS QHSRSELLNL
Sbjct: 1   MISVKELVEKSSIITIPQNFIRLDQEPSSTSDPSTFPTPPTIDMTRLLSLQHSRSELLNL 60

Query: 61  HSACKQWGLFQLVNHGVSSSLLEEVKSEIEGFFELPLDEKMKFCMKAGEFEGYGTVVRSM 120
           HSACKQWGLFQLVNHGVS SLL E+K EIE FFELPL+EKMK+ MK+GE EGYGTVVRSM
Sbjct: 61  HSACKQWGLFQLVNHGVSCSLLGELKHEIEEFFELPLEEKMKYGMKSGEVEGYGTVVRSM 120

Query: 121 DQKLDWGDRFYMITNPLSRRKPHLLPHFPPSLRNTLESYLKATKKIAMSLLRMMAGNLEM 180
           +QKLDWGDRFY+ITNPLSRRKPHLLP  P SLRN LE YLK  KKIAM+L RM+AGNLE+
Sbjct: 121 EQKLDWGDRFYIITNPLSRRKPHLLPLLPLSLRNALEWYLKEMKKIAMALFRMIAGNLEI 180

Query: 181 EVRKMEELFEDGMEAVRMTYYPPCPRPELVVGLRPHSDATGVTILNQLNGVEGLQVKKDG 240
           EVRK+E+LFEDGMEA+RM+YYPPCP PELVVGLRPHSDA+G+TILNQLNGVEGLQVKKDG
Sbjct: 181 EVRKVEDLFEDGMEAMRMSYYPPCPTPELVVGLRPHSDASGLTILNQLNGVEGLQVKKDG 240

Query: 241 TWFPVSFIPDGLMVNVGDVVEIMSNGVYNSIEHRAAVNSEKGRMSIAVFYNPRFEAEIGP 300
            WFPVSFIPD  +VNVGD++EIMSNG+YNSIEHRA VNSEK RMSIA+FYNPRF+AEI P
Sbjct: 241 IWFPVSFIPDAFIVNVGDILEIMSNGLYNSIEHRATVNSEKERMSIAIFYNPRFDAEIAP 300

Query: 301 QT--QSPPLFRRIVMEDYFKDFFTQSFSGKSHLERMKIPPTNQPN 344
            T  Q+PPLF+ I+MEDYFKDFFTQSF+GKSHL+RMKIP T+QPN
Sbjct: 301 LTAFQNPPLFKNILMEDYFKDFFTQSFNGKSHLDRMKIPTTHQPN 345

[Close]

BLAST of CmoCh03G013560 vs. TAIR 10
Match: AT1G78550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 322.4 bits (825), Expect = 4.4e-88
Identity = 162/338 (47.93%), Postives = 225/338 (66.57%), Query Frame = 0


Query: 6   ELVESSSKINIPQNFIRLDQEPSSTSHPSTFPAP-PTIDMSRLLSPQHSRSELLNLHSAC 65
           E+V+  +   IP  ++R+DQE +   + S+  +  P IDM+RL S     SEL  L  AC
Sbjct: 19  EIVKEKNFTTIPPRYVRVDQEKTEILNDSSLSSEIPVIDMTRLCSVSAMDSELKKLDFAC 78

Query: 66  KQWGLFQLVNHGVSSSLLEEVKSEIEGFFELPLDEKMKFCMKAGEFEGYGTV-VRSMDQK 125
           + WG FQLVNHG+ SS LE++++E++ FF LP+ EK K   ++GEFEG+G V + S +QK
Sbjct: 79  QDWGFFQLVNHGIDSSFLEKLETEVQEFFNLPMKEKQKLWQRSGEFEGFGQVNIVSENQK 138

Query: 126 LDWGDRFYMITNPLSRRKPHLLPHFPPSLRNTLESYLKATKKIAMSLLRMMAGNLEMEVR 185
           LDWGD F + T P+  RK HL    PP  R TLE+Y    K IA  L   MA  LE++  
Sbjct: 139 LDWGDMFILTTEPIRSRKSHLFSKLPPPFRETLETYSSEVKSIAKILFAKMASVLEIKHE 198

Query: 186 KMEELFEDGMEAVRMTYYPPCPRPELVVGLRPHSDATGVTILNQLNGVEGLQVKKDGTWF 245
           +ME+LF+D  +++++ YYPPCP+P+ V+GL  HSDA G+TIL Q+N VEGLQ+KKDG W 
Sbjct: 199 EMEDLFDDVWQSIKINYYPPCPQPDQVMGLTQHSDAAGLTILLQVNQVEGLQIKKDGKWV 258

Query: 246 PVSFIPDGLMVNVGDVVEIMSNGVYNSIEHRAAVNSEKGRMSIAVFYNPRFEAEIGP--- 305
            V  + D L+VNVG+++EI++NG Y SIEHRA VNSEK R+S+A+F++P  E  I P   
Sbjct: 259 VVKPLRDALVVNVGEILEIITNGRYRSIEHRAVVNSEKERLSVAMFHSPGKETIIRPAKS 318

Query: 306 --QTQSPPLFRRIVMEDYFKDFFTQSFSGKSHLERMKI 337
               Q   LF+ +  ++YF  FFTQ  +GKSHL+ M+I
Sbjct: 319 LVDRQKQCLFKSMSTQEYFDAFFTQKLNGKSHLDLMRI 356

[Close]

BLAST of CmoCh03G013560 vs. TAIR 10
Match: AT1G17020.1 (senescence-related gene 1 )

HSP 1 Score: 313.9 bits (803), Expect = 1.6e-85
Identity = 155/343 (45.19%), Postives = 222/343 (64.72%), Query Frame = 0


Query: 3   SLKELVESSSKINIPQNFIRLDQEPSSTSHPSTFPAP-PTIDMSRLLSPQHSRSELLNLH 62
           S++E+V+  +   +P  ++R DQ+ +            P IDM RL S     SE+  L 
Sbjct: 16  SVQEMVKEKTITTVPPRYVRSDQDKTEVDDDFDVKIEIPIIDMKRLCSSTTMDSEVEKLD 75

Query: 63  SACKQWGLFQLVNHGVSSSLLEEVKSEIEGFFELPLDEKMKFCMKAGEFEGYG-TVVRSM 122
            ACK+WG FQLVNHG+ SS L++VKSEI+ FF LP++EK KF  +  E EG+G   V S 
Sbjct: 76  FACKEWGFFQLVNHGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGFGQAFVVSE 135

Query: 123 DQKLDWGDRFYMITNPLSRRKPHLLPHFPPSLRNTLESYLKATKKIAMSLLRMMAGNLEM 182
           DQKLDW D F+    P+  RKPHL P  P   R+TLE Y    + +A  L+  MA  LE+
Sbjct: 136 DQKLDWADLFFHTVQPVELRKPHLFPKLPLPFRDTLEMYSSEVQSVAKILIAKMARALEI 195

Query: 183 EVRKMEELFE--DGMEAVRMTYYPPCPRPELVVGLRPHSDATGVTILNQLNGVEGLQVKK 242
           +  ++E+LF+  D ++++RM YYPPCP+P+ V+GL PHSD+ G+T+L Q+N VEGLQ+KK
Sbjct: 196 KPEELEKLFDDVDSVQSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQVNDVEGLQIKK 255

Query: 243 DGTWFPVSFIPDGLMVNVGDVVEIMSNGVYNSIEHRAAVNSEKGRMSIAVFYNPRFEAEI 302
           DG W PV  +P+  +VN+GDV+EI++NG Y SIEHR  VNSEK R+SIA F+N     E+
Sbjct: 256 DGKWVPVKPLPNAFIVNIGDVLEIITNGTYRSIEHRGVVNSEKERLSIATFHNVGMYKEV 315

Query: 303 GP-----QTQSPPLFRRIVMEDYFKDFFTQSFSGKSHLERMKI 337
           GP     + Q    F+R+ M++Y    F+++  GK++L+ ++I
Sbjct: 316 GPAKSLVERQKVARFKRLTMKEYNDGLFSRTLDGKAYLDALRI 358

[Close]

BLAST of CmoCh03G013560 vs. TAIR 10
Match: AT1G17010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 298.5 bits (763), Expect = 6.8e-81
Identity = 158/343 (46.06%), Postives = 218/343 (63.56%), Query Frame = 0


Query: 3   SLKELVESSSKINIPQNFIRLDQEPSS-TSHPSTFPAP-PTIDMSRLLSPQHSRSELLNL 62
           S++E+V+      +P  ++R DQ+ +    H S   +  P IDM+RL S     SE+  L
Sbjct: 16  SVQEMVKDKMITTVPPRYVRYDQDKTEVVVHDSGLISEIPIIDMNRLCSSTAVDSEVEKL 75

Query: 63  HSACKQWGLFQLVNHGVSSSLLEEVKSEIEGFFELPLDEKMKFCMKAGEFEGYG-TVVRS 122
             ACK++G FQLVNHG+  S L+++KSEI+ FF LP++EK K        EG+G   V S
Sbjct: 76  DFACKEYGFFQLVNHGIDPSFLDKIKSEIQDFFNLPMEEKKKLWQTPAVMEGFGQAFVVS 135

Query: 123 MDQKLDWGDRFYMITNPLSRRKPHLLPHFPPSLRNTLESYLKATKKIAMSLLRMMAGNLE 182
            DQKLDW D F++I  P+  RK HL P  P   R+TL+ Y    K IA  LL  MA  L+
Sbjct: 136 EDQKLDWADLFFLIMQPVQLRKRHLFPKLPLPFRDTLDMYSTRVKSIAKILLAKMAKALQ 195

Query: 183 MEVRKMEELF-EDGMEAVRMTYYPPCPRPELVVGLRPHSDATGVTILNQLNGVEGLQVKK 242
           ++  ++EE+F +D M+++RM YYPPCP+P LV GL PHSDA G+TIL Q+N V+GLQ+KK
Sbjct: 196 IKPEEVEEIFGDDMMQSMRMNYYPPCPQPNLVTGLIPHSDAVGLTILLQVNEVDGLQIKK 255

Query: 243 DGTWFPVSFIPDGLMVNVGDVVEIMSNGVYNSIEHRAAVNSEKGRMSIAVFYNPRFEAEI 302
           +G WF V  + +  +VNVGDV+EI++NG Y SIEHRA VN EK R+SIA F+N   + EI
Sbjct: 256 NGKWFFVKPLQNAFIVNVGDVLEIITNGTYRSIEHRAMVNLEKERLSIATFHNTGMDKEI 315

Query: 303 GP-----QTQSPPLFRRIVMEDYFKDFFTQSFSGKSHLERMKI 337
           GP     Q Q    FR +  +DY    F++   GK++L+ M+I
Sbjct: 316 GPARSLVQRQEAAKFRSLKTKDYLNGLFSRELKGKAYLDAMRI 358

[Close]

BLAST of CmoCh03G013560 vs. TAIR 10
Match: AT4G25310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 298.5 bits (763), Expect = 6.8e-81
Identity = 156/342 (45.61%), Postives = 217/342 (63.45%), Query Frame = 0


Query: 3   SLKELV-ESSSKINIPQNFIRLDQEPSSTSHPSTFPAPPTIDMSRLLSPQHSRSELLNLH 62
           S++E+V E      +P  ++R DQE    +  S     P IDMS L S     SE+  L 
Sbjct: 15  SVQEMVKEKVITTVLPPRYVRSDQEKGEAAIDSGENQIPIIDMSLLSSSTSMDSEIDKLD 74

Query: 63  SACKQWGLFQLVNHGVSSSLLEEVKSEIEGFFELPLDEKMKFCMKAGEFEGYG-TVVRSM 122
            ACK+WG FQLVNHG+    L++ KS+I+ FF LP++EK K   + G+ EG+G   V S 
Sbjct: 75  FACKEWGFFQLVNHGMD---LDKFKSDIQDFFNLPMEEKKKLWQQPGDIEGFGQAFVFSE 134

Query: 123 DQKLDWGDRFYMITNPLSRRKPHLLPHFPPSLRNTLESYLKATKKIAMSLLRMMAGNLEM 182
           +QKLDW D F++   P+  RKPHL P  P   R+TL++Y    K IA  L   +A  L++
Sbjct: 135 EQKLDWADVFFLTMQPVPLRKPHLFPKLPLPFRDTLDTYSAELKSIAKVLFAKLASALKI 194

Query: 183 EVRKMEELFEDGM-EAVRMTYYPPCPRPELVVGLRPHSDATGVTILNQLNGVEGLQVKKD 242
           +  +ME+LF+D + + +RM YYPPCP P+  +GL PHSDATG+TIL Q+N VEGLQ+KKD
Sbjct: 195 KPEEMEKLFDDELGQRIRMNYYPPCPEPDKAIGLTPHSDATGLTILLQVNEVEGLQIKKD 254

Query: 243 GTWFPVSFIPDGLMVNVGDVVEIMSNGVYNSIEHRAAVNSEKGRMSIAVFYNPRFEAEIG 302
           G W  V  +P+ L+VNVGD++EI++NG Y SIEHR  VNSEK R+S+A F+N  F  EIG
Sbjct: 255 GKWVSVKPLPNALVVNVGDILEIITNGTYRSIEHRGVVNSEKERLSVASFHNTGFGKEIG 314

Query: 303 P-----QTQSPPLFRRIVMEDYFKDFFTQSFSGKSHLERMKI 337
           P     +     LF+ +  E+YF   F++   GK++L+ M+I
Sbjct: 315 PMRSLVERHKGALFKTLTTEEYFHGLFSRELDGKAYLDVMRI 353

[Close]

BLAST of CmoCh03G013560 vs. TAIR 10
Match: AT4G25300.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 296.2 bits (757), Expect = 3.4e-80
Identity = 151/342 (44.15%), Postives = 216/342 (63.16%), Query Frame = 0


Query: 3   SLKELVESSSKINIPQNFIRLDQEPSSTSHPSTFPAP-PTIDMSRLLSPQHSRSELLNLH 62
           S++E+V+      +P  ++R DQ+ +  +  S      P IDMS L S     SE+  L 
Sbjct: 15  SVQEMVKEKMITTVPPRYVRSDQDVAEIAVDSGLRNQIPIIDMSLLCSSTSMDSEIDKLD 74

Query: 63  SACKQWGLFQLVNHGVSSSLLEEVKSEIEGFFELPLDEKMKFCMKAGEFEGYGTV-VRSM 122
           SACK+WG FQLVNHG+ SS L +VKSE++ FF LP++EK     +  E EG+G V V S 
Sbjct: 75  SACKEWGFFQLVNHGMESSFLNKVKSEVQDFFNLPMEEKKNLWQQPDEIEGFGQVFVVSE 134

Query: 123 DQKLDWGDRFYMITNPLSRRKPHLLPHFPPSLRNTLESYLKATKKIAMSLLRMMAGNLEM 182
           +QKLDW D F++   P+  RKPHL P  P   R+TL+ Y    K IA  LL  +A  L++
Sbjct: 135 EQKLDWADMFFLTMQPVRLRKPHLFPKLPLPFRDTLDMYSAEVKSIAKILLGKIAVALKI 194

Query: 183 EVRKMEELFEDGM-EAVRMTYYPPCPRPELVVGLRPHSDATGVTILNQLNGVEGLQVKKD 242
           +  +M++LF+D + + +R+ YYP CP P+ V+GL PHSD+TG+TIL Q N VEGLQ+KK+
Sbjct: 195 KPEEMDKLFDDELGQRIRLNYYPRCPEPDKVIGLTPHSDSTGLTILLQANEVEGLQIKKN 254

Query: 243 GTWFPVSFIPDGLMVNVGDVVEIMSNGVYNSIEHRAAVNSEKGRMSIAVFYNPRFEAEIG 302
             W  V  +P+ L+VNVGD++EI++NG Y SIEHR  VNSEK R+S+A F+N     EIG
Sbjct: 255 AKWVSVKPLPNALVVNVGDILEIITNGTYRSIEHRGVVNSEKERLSVAAFHNIGLGKEIG 314

Query: 303 P-----QTQSPPLFRRIVMEDYFKDFFTQSFSGKSHLERMKI 337
           P     +      F+ +  E+YF   F++   GK++L+ M++
Sbjct: 315 PMRSLVERHKAAFFKSVTTEEYFNGLFSRELDGKAYLDVMRL 356

The following BLAST results are available for this feature:

BLAST of CmoCh03G013560 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
D4N502	9.0e-86	50.33	Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1	[more]
Q39224	2.2e-84	45.19	Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1	[more]
D4N501	2.1e-79	48.54	Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum OX=34...	[more]
D4N500	1.8e-78	45.83	Thebaine 6-O-demethylase OS=Papaver somniferum OX=3469 GN=T6ODM PE=1 SV=1	[more]
Q94LP4	8.7e-65	39.39	2-oxoglutarate-dependent dioxygenase 11 OS=Oryza sativa subsp. japonica OX=39947...	[more]

BLAST of CmoCh03G013560 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ED48	7.6e-197	100.00	codeine O-demethylase-like OS=Cucurbita moschata OX=3662 GN=LOC111433087 PE=3 SV...	[more]
A0A6J1INU8	2.2e-191	96.79	codeine O-demethylase-like OS=Cucurbita maxima OX=3661 GN=LOC111478599 PE=3 SV=1	[more]
A0A5A7UA06	4.8e-159	80.64	Codeine O-demethylase-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A6J1CKJ4	5.2e-145	75.73	protein SRG1-like OS=Momordica charantia OX=3673 GN=LOC111012449 PE=3 SV=1	[more]
A0A1S3AYY1	2.5e-139	80.53	codeine O-demethylase-like OS=Cucumis melo OX=3656 GN=LOC103484411 PE=3 SV=1	[more]

BLAST of CmoCh03G013560 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022925781.1	1.6e-196	100.00	codeine O-demethylase-like [Cucurbita moschata] >KAG7034678.1 Codeine O-demethyl...	[more]
KAG6581392.1	4.3e-194	98.83	Protein SRG1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022978731.1	4.4e-191	96.79	codeine O-demethylase-like [Cucurbita maxima]	[more]
XP_023544159.1	1.3e-190	96.79	codeine O-demethylase-like [Cucurbita pepo subsp. pepo]	[more]
XP_038883393.1	4.8e-161	82.32	protein SRG1-like [Benincasa hispida]	[more]

BLAST of CmoCh03G013560 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G78550.1	4.4e-88	47.93	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G17020.1	1.6e-85	45.19	senescence-related gene 1	[more]
AT1G17010.1	6.8e-81	46.06	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G25310.1	6.8e-81	45.61	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G25300.1	3.4e-80	44.15	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..326 e-value: 4.3E-93 score: 314.2
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 194..292 e-value: 5.5E-23 score: 81.4
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 40..138 e-value: 3.1E-25 score: 89.2
None	No IPR available	PANTHER	PTHR47991:SF3	PROTEIN SRG1-LIKE	coord: 3..338
None	No IPR available	PANTHER	PTHR47991	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE	coord: 3..338
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 12..327
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 192..292 score: 12.169368

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh03G013560.1	CmoCh03G013560.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0016491	oxidoreductase activity