CmoCh03G012040 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh03G012040
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionGDSL esterase/lipase
LocationCmo_Chr03: 9383657 .. 9385006 (-)
RNA-Seq ExpressionCmoCh03G012040
SyntenyCmoCh03G012040
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCATTTTGTTCTCCGCCTTCTCTTCCCTCTTTTCCTTTCCATCTCTCTCTTCTCAGGTTTGGATTCTGTGCTTATTTCATTTACTTCACAATTTCTGTTTCTTCTCTCTCTGATTTCTTCCATTTAGGACGAGTATCGGCTGGTGGCGTTTACAATCACCACCTCCGCCACCGTTTCCGGCCTACAAAGCTGTTTGTTTTTGGGGATTCCTATACTGATACCGGCAACATTCTTTTTCCCATTTCCCCCGGTCTGCAATTTCCTTATGGAATTACCTTTCCAGGAAAACCCATTGGCCGTTTCTCCGATGGTCGTGTTCTCACCGATTTTGCAGGTAAACAAATCTGTGTCTCTGTTTCTGTCTCTCTGTTTCGCCGATTTTCCGACCGTCCTGTTCTTACTAAAAACAAATGGCTGTCAAAGGAGGCCTTAAAATGATCTTAATCTCGAAATGTAATGGAACGTATGGGAATAATTGAATGCAGCGAGGCATGTCGGGTTGAAATCTCCGATCCCATTCAGTGTCCGAAACAGAGTCGGGTTGAGAAGATTCAAAGAAACAGGAGTAAACTTTGCATTTGGGGGAACCGGCGTCTTCGACACAGAGTATCCTTTGCCAAACATGACAACCCAGATCGACTTATTTCAACAGCTCCGACATGGCGATTCCGTATTCACCAATACAGACGCTCAATCCTCTGTCGCACTCGTATCTGCTTCCGGCAACGACTATTCCTTTTACTTCGCCATGAACAGCTCTAGACAGGTATCAATCAACTCCGCAATCTAACCCCAAGAGAATCACAAACTGAAATTCCGATGTGTTTTACAGGGATTGAAACCGTTCATAATGTCGGTAGTGAATCAGATCATCGTGAATTTGAAACGCATTGGGGGGTTGGGAGTGAAGAAGATAGCGGTGACAGGGCTAGGCCCATTGGGATGTCTTCCGGTATTTACAGCGCCCTCATCATACAAGAAATGCAATGAAACAGTAAACTCGTTGGTGCAGTTTCACAACTTTTTGTTGAAGGAAGCTGTGAAGAAGCTGAACCAAGAAACGAAGCATTCTTCGAAGGTGTTTATTGTTGATGTGTATGGGGCGTTCATGTCCATAATTGAAGGGCGGAAGAGATACAACAATGGAGAGTTCAAGACGCCATTGAAGCCGTGTTGTTTTGGAGTGAAGAGTGGATTTGATTGTGGGAGTGTGGATGGAGAAGGGAAGAAGATGTTTGTGTTATGCAAGGATCGGAAAGCAGCCTTCTTTTGGGATTCAGTGCATCCTACGCAACAAGGTTGGGCTGCTGCATTTTCTGCTTTTAAAAGCTTTCGCAACCATTTTTAA

mRNA sequence

ATGCATTTTGTTCTCCGCCTTCTCTTCCCTCTTTTCCTTTCCATCTCTCTCTTCTCAGGACGAGTATCGGCTGGTGGCGTTTACAATCACCACCTCCGCCACCGTTTCCGGCCTACAAAGCTGTTTGTTTTTGGGGATTCCTATACTGATACCGGCAACATTCTTTTTCCCATTTCCCCCGGTCTGCAATTTCCTTATGGAATTACCTTTCCAGGAAAACCCATTGGCCGTTTCTCCGATGGTCGTGTTCTCACCGATTTTGCAGCGAGGCATGTCGGGTTGAAATCTCCGATCCCATTCAGTGTCCGAAACAGAGTCGGGTTGAGAAGATTCAAAGAAACAGGAGTAAACTTTGCATTTGGGGGAACCGGCGTCTTCGACACAGAGTATCCTTTGCCAAACATGACAACCCAGATCGACTTATTTCAACAGCTCCGACATGGCGATTCCGTATTCACCAATACAGACGCTCAATCCTCTGTCGCACTCGTATCTGCTTCCGGCAACGACTATTCCTTTTACTTCGCCATGAACAGCTCTAGACAGGGATTGAAACCGTTCATAATGTCGGTAGTGAATCAGATCATCGTGAATTTGAAACGCATTGGGGGGTTGGGAGTGAAGAAGATAGCGGTGACAGGGCTAGGCCCATTGGGATGTCTTCCGGTATTTACAGCGCCCTCATCATACAAGAAATGCAATGAAACAGTAAACTCGTTGGTGCAGTTTCACAACTTTTTGTTGAAGGAAGCTGTGAAGAAGCTGAACCAAGAAACGAAGCATTCTTCGAAGGTGTTTATTGTTGATGTGTATGGGGCGTTCATGTCCATAATTGAAGGGCGGAAGAGATACAACAATGGAGAGTTCAAGACGCCATTGAAGCCGTGTTGTTTTGGAGTGAAGAGTGGATTTGATTGTGGGAGTGTGGATGGAGAAGGGAAGAAGATGTTTGTGTTATGCAAGGATCGGAAAGCAGCCTTCTTTTGGGATTCAGTGCATCCTACGCAACAAGGTTGGGCTGCTGCATTTTCTGCTTTTAAAAGCTTTCGCAACCATTTTTAA

Coding sequence (CDS)

ATGCATTTTGTTCTCCGCCTTCTCTTCCCTCTTTTCCTTTCCATCTCTCTCTTCTCAGGACGAGTATCGGCTGGTGGCGTTTACAATCACCACCTCCGCCACCGTTTCCGGCCTACAAAGCTGTTTGTTTTTGGGGATTCCTATACTGATACCGGCAACATTCTTTTTCCCATTTCCCCCGGTCTGCAATTTCCTTATGGAATTACCTTTCCAGGAAAACCCATTGGCCGTTTCTCCGATGGTCGTGTTCTCACCGATTTTGCAGCGAGGCATGTCGGGTTGAAATCTCCGATCCCATTCAGTGTCCGAAACAGAGTCGGGTTGAGAAGATTCAAAGAAACAGGAGTAAACTTTGCATTTGGGGGAACCGGCGTCTTCGACACAGAGTATCCTTTGCCAAACATGACAACCCAGATCGACTTATTTCAACAGCTCCGACATGGCGATTCCGTATTCACCAATACAGACGCTCAATCCTCTGTCGCACTCGTATCTGCTTCCGGCAACGACTATTCCTTTTACTTCGCCATGAACAGCTCTAGACAGGGATTGAAACCGTTCATAATGTCGGTAGTGAATCAGATCATCGTGAATTTGAAACGCATTGGGGGGTTGGGAGTGAAGAAGATAGCGGTGACAGGGCTAGGCCCATTGGGATGTCTTCCGGTATTTACAGCGCCCTCATCATACAAGAAATGCAATGAAACAGTAAACTCGTTGGTGCAGTTTCACAACTTTTTGTTGAAGGAAGCTGTGAAGAAGCTGAACCAAGAAACGAAGCATTCTTCGAAGGTGTTTATTGTTGATGTGTATGGGGCGTTCATGTCCATAATTGAAGGGCGGAAGAGATACAACAATGGAGAGTTCAAGACGCCATTGAAGCCGTGTTGTTTTGGAGTGAAGAGTGGATTTGATTGTGGGAGTGTGGATGGAGAAGGGAAGAAGATGTTTGTGTTATGCAAGGATCGGAAAGCAGCCTTCTTTTGGGATTCAGTGCATCCTACGCAACAAGGTTGGGCTGCTGCATTTTCTGCTTTTAAAAGCTTTCGCAACCATTTTTAA

Protein sequence

MHFVLRLLFPLFLSISLFSGRVSAGGVYNHHLRHRFRPTKLFVFGDSYTDTGNILFPISPGLQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKETGVNFAFGGTGVFDTEYPLPNMTTQIDLFQQLRHGDSVFTNTDAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCNETVNSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGAFMSIIEGRKRYNNGEFKTPLKPCCFGVKSGFDCGSVDGEGKKMFVLCKDRKAAFFWDSVHPTQQGWAAAFSAFKSFRNHF
Homology
BLAST of CmoCh03G012040 vs. ExPASy Swiss-Prot
Match: Q9LZS7 (GDSL esterase/lipase At5g03610 OS=Arabidopsis thaliana OX=3702 GN=At5g03610 PE=2 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 4.3e-99
Identity = 183/352 (51.99%), Postives = 243/352 (69.03%), Query Frame = 0

Query: 1   MHFVLRLLFPL--FLSISLFSGRVSA--GGVYNHHLRHRFRPTKLFVFGDSYTDTGNILF 60
           M  +++L F L  FL  SL  G ++   G   NHHL + FRPTKLFVFGDSY DTGNI  
Sbjct: 1   MDSLIKLFFCLFIFLCTSLLFGEINGVEGSNQNHHL-YPFRPTKLFVFGDSYADTGNIKK 60

Query: 61  PISPGLQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKETGV 120
             S   +FPYGITFPGKP GRFSDGRV TDF A+ VG+KSPIP+  ++  G +R  + G+
Sbjct: 61  AFSSSWKFPYGITFPGKPAGRFSDGRVATDFLAKFVGIKSPIPYFWKDYAGKKRL-QYGM 120

Query: 121 NFAFGGTGVFDTEYPLPNMTTQIDLFQQLRHGDSVFTNTDAQSSVALVSASGNDYSFYFA 180
           NFA+GGTGVF+T+ PLPNMTTQID+FQ +     ++   +  SSVALVS +GNDYS + A
Sbjct: 121 NFAYGGTGVFNTQTPLPNMTTQIDIFQNILTTGDIYYPPELTSSVALVSVAGNDYSNFIA 180

Query: 181 MNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCNET 240
           +N        FI  VV+Q  VNL+RI  LGVKKIAV  L PLGCLP FT  +S+++CNET
Sbjct: 181 LNRPASEFPAFIKQVVDQTEVNLRRIHALGVKKIAVPSLQPLGCLPPFTFVTSFQRCNET 240

Query: 241 VNSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGAFMSIIEGR-KRYNNGEFKTPLKP 300
            N+LV  HN LL++ V KLN ETK S+ + I+D+Y AF+++ + +     +  F++PLKP
Sbjct: 241 QNALVNLHNNLLQQVVAKLNNETKQSTFI-ILDLYNAFLTVFKNKGSNPGSTRFESPLKP 300

Query: 301 CCFGVKSGFDCGSVDGEGKKMFVLCKDRKAAFFWDSVHPTQQGWAAAFSAFK 348
           CC GV   ++CGSVD +G K +++C + K AFFWD +HPT++GW + +S  +
Sbjct: 301 CCVGVSREYNCGSVDEKGVKKYIVCDNPKTAFFWDGLHPTEEGWRSVYSVLR 349

BLAST of CmoCh03G012040 vs. ExPASy Swiss-Prot
Match: Q9SF94 (GDSL esterase/lipase At3g09930 OS=Arabidopsis thaliana OX=3702 GN=At3g09930 PE=2 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 2.9e-87
Identity = 164/342 (47.95%), Postives = 226/342 (66.08%), Query Frame = 0

Query: 6   RLLFPLFLSISLFSGRVSAGGVYNHHLR-HRFRPTKLFVFGDSYTDTGNILFPISPGLQF 65
           +LL  LFL  S  S  + A   Y  H    R RP +LFVFGDSY DTGNI   +S   + 
Sbjct: 5   KLLISLFL-FSFSSFFLGAESDYPQHSNLKRLRPNRLFVFGDSYADTGNIRKSLSDSWKI 64

Query: 66  PYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKETGVNFAFGGTG 125
           PYGITFP KP GRFSDGRV TDF AR++G+KSPIP++ ++  G  R    G+N+A+GGTG
Sbjct: 65  PYGITFPQKPSGRFSDGRVATDFLARYLGIKSPIPYTWKDYAGKERLL-YGMNYAYGGTG 124

Query: 126 VFDT-EYPLPNMTTQIDLFQQLRHGDSVFTNTDAQSSVALVSASGNDYSFYFAMNSSRQG 185
           VF T + PLPNMTTQID FQ++    ++++ +D  SS+ALVS +GNDY+ + A+      
Sbjct: 125 VFKTKDNPLPNMTTQIDYFQRVLAAGNIYSPSDLPSSLALVSVAGNDYATFLALKRPLTE 184

Query: 186 LKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCNETVNSLVQF 245
           L  F+  VV+QI VN  RI  LGV KI +  + PLGCLP  T  +S+++CN T N+    
Sbjct: 185 LPAFMKQVVDQIAVNAMRIHKLGVNKIVIPSMQPLGCLPSITVFNSFQRCNATDNASTNL 244

Query: 246 HNFLLKEAVKKLNQETKHSSKVFIVDVYGAFMSIIEGR-KRYNNGEFKTPLKPCCFGVKS 305
           HN+LL +A+ +LN ETK S+ V ++D Y AF+++ + +        F  PLKPCC GV S
Sbjct: 245 HNYLLHKAIARLNNETKPSTFV-VLDHYNAFLTVFKNKGPEPGVSRFGNPLKPCCVGVNS 304

Query: 306 GFDCGSVDGEGKKMFVLCKDRKAAFFWDSVHPTQQGWAAAFS 345
            +DC +VD +G+K +++C+D KAAFFWD  HP+++GW + +S
Sbjct: 305 SYDCSNVDEKGEKKYIICEDPKAAFFWDIFHPSEEGWRSVYS 343

BLAST of CmoCh03G012040 vs. ExPASy Swiss-Prot
Match: Q9LZS9 (GDSL esterase/lipase At5g03590 OS=Arabidopsis thaliana OX=3702 GN=At5g03590 PE=3 SV=2)

HSP 1 Score: 261.5 bits (667), Expect = 1.3e-68
Identity = 153/351 (43.59%), Postives = 209/351 (59.54%), Query Frame = 0

Query: 1   MHFVLRLLFPLFLSISLFSGRVSAGGVYNHHLRHRFRPTKLFVFGDSYTDTGNILFPISP 60
           MH++++    LF S+SLF G    G V ++      +  KLFVFG+SY DTGN + P + 
Sbjct: 1   MHYLMK----LFFSLSLFFG--INGAVGSNQPSQANKNPKLFVFGNSYADTGN-MKPTAL 60

Query: 61  GLQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSV--RNRVGLRRFKETGVNF 120
             + PYGITFPGKP GR+SDG   TDF A+ +G K P  +    + +V L R    G+NF
Sbjct: 61  SWKLPYGITFPGKPSGRYSDGLTATDFLAKQLGAKLPYLWRTHGKKKVKLNR----GMNF 120

Query: 121 AFGGTGVFDTEYP-LPNMTTQIDLFQQLRHGDSVFT-NTDAQSSVALVSASGNDYSFYFA 180
           AFGG+ VFD+     PN++TQ+     L     V+T + D  SS AL+S SG DY  +  
Sbjct: 121 AFGGSEVFDSPVDRSPNISTQVGFLVNLALARRVYTIDGDLASSYALLSYSGTDYYGFID 180

Query: 181 MNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCNET 240
            N +      F+  +V  I  +L  + GL  K IAVT L PLGCLP  T  SS++ CNE+
Sbjct: 181 QNPNMAAYPAFVEFIVEDIQYSLGIMNGLKFKNIAVTSLHPLGCLPRVTVASSFRSCNES 240

Query: 241 VNSLVQFHNFLLKEAVKKLNQETKHSSK---VFIVDVYGAFMSIIEGRKRYNNGEFKTPL 300
            + LV+ HN  LK+AV KLN+E K  +K     IVD++ AFM+I+E   +  N  FK+PL
Sbjct: 241 YSDLVRLHNESLKKAVAKLNKEDKFRTKGDRFVIVDLHKAFMTILE---KKGNKRFKSPL 300

Query: 301 KPCCFGVKSGFDCGSVDGEGKKMFVLCKDRKAAFFWDSVHPTQQGWAAAFS 345
           KPCC G     DC  +D +G K + LC D K+AFFWD ++PTQ+GW + +S
Sbjct: 301 KPCCEG-----DCARMDMKGAKKYTLCNDPKSAFFWDEINPTQEGWRSIYS 332

BLAST of CmoCh03G012040 vs. ExPASy Swiss-Prot
Match: Q9LZS8 (GDSL esterase/lipase At5g03600 OS=Arabidopsis thaliana OX=3702 GN=At5g03600 PE=3 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 1.3e-66
Identity = 141/322 (43.79%), Postives = 195/322 (60.56%), Query Frame = 0

Query: 29  NHHLRHRFRPTKLFVFGDSYTDTGNILFPISPGLQFPYGITFPGKPIGRFSDGRVLTDFA 88
           N  LR R  P KLFVFGDSY DTGN     +     PYGITFPGKP GR+ DG + TDF 
Sbjct: 4   NQLLRPRKVP-KLFVFGDSYADTGNTKRD-TEAWAIPYGITFPGKPSGRYCDGLIATDFL 63

Query: 89  ARHVGLKSPIPFSVRNR-VGLRRFKETGVNFAFGGTGVFDT--EYPLPNMTTQIDLFQQL 148
            + +G +SP  +    R  GL+R    G+NFAFGG+ + D+    P PN+T Q++    L
Sbjct: 64  EKVLGAESPYLYRTHGRDKGLKR----GMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDL 123

Query: 149 RHGDSVFTNTDAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGL 208
                V+ +    S V+L+S +G DY +Y   N    GLK  +  VV+ + VN+  +GGL
Sbjct: 124 VLAGRVYGDI-TPSDVSLISYAGGDYIYYIDQNRPAAGLKALVEKVVDNLRVNMIVLGGL 183

Query: 209 GVKKIAVTGLGPLGCLPVFTAPSSYKKCNETVNSLVQFHNFLLKEAVKKLNQET---KHS 268
             KKIAVT L P+GCLP +T+ SS+K CNE+ ++LV+ HN LLK+ V KLN+++   K  
Sbjct: 184 LFKKIAVTSLQPIGCLPSYTSASSFKSCNESQSALVELHNKLLKKVVAKLNEQSRVMKKE 243

Query: 269 SKVFIVDVYGAFMSIIEGRKRYNNGEFKTPLKPCCFGVKSGFDCGSVDGEGKKMFVLCKD 328
              FI+D++ AFM+++   K   +  FK P+K CC G      CG    +G K++ LC D
Sbjct: 244 QHFFIIDIHNAFMTVM---KNKGSKRFKNPMKSCCEGY-----CGR-SSDGGKLYTLCDD 303

Query: 329 RKAAFFWDSVHPTQQGWAAAFS 345
            K+ FFWD+VHPTQ+GW + +S
Sbjct: 304 PKSFFFWDAVHPTQEGWRSIYS 309

BLAST of CmoCh03G012040 vs. ExPASy Swiss-Prot
Match: Q8RWJ4 (GDSL esterase/lipase At2g36325 OS=Arabidopsis thaliana OX=3702 GN=At2g36325 PE=2 SV=2)

HSP 1 Score: 248.1 bits (632), Expect = 1.5e-64
Identity = 137/312 (43.91%), Postives = 186/312 (59.62%), Query Frame = 0

Query: 36  FRPTKLFVFGDSYTDTGNILFPISPGLQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLK 95
           F+PTKLFVFGDSY DTGN  F I P  +FP GITFPG P GRFSDGRV TD+ A+++G++
Sbjct: 43  FKPTKLFVFGDSYADTGNTPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYLAKYIGVR 102

Query: 96  SPIPFSVRNRVGLRRFKETGVNFAFGGTGVFDTEYPL-PNMTTQIDLFQQLRHGDSVFTN 155
           +PI +        R   + G+NFA+GG G F+T + L P  + QID F+QL    +V++ 
Sbjct: 103 TPITYKWGKYGRPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQLLM-RNVYSP 162

Query: 156 TDAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTG 215
            D  SSVA  S  GNDY  Y   N S +G       VV QI++++KRI  LGV+K+ V  
Sbjct: 163 ADLNSSVAFFSIIGNDYLTYDRRNGSEEGRSALTRKVVKQILLDVKRIKDLGVRKVLVAL 222

Query: 216 LGPLGCLPVFTAPSSYKKCNETVNSLVQFHNFLLKEAVKKLN-QETKHSSKVFI-VDVYG 275
             P  CLP    P   K C +T ++    HN LL++ + KLN +E  ++ K F+ +D+Y 
Sbjct: 223 SPPQKCLPKLVTP---KGC-DTNDTSTYLHNSLLRKGLIKLNDKEINNNDKSFMTLDLYN 282

Query: 276 AFMSIIEGRKRYNNGEFKTPLKPCCFGVKSGFDCGSVDGEGKKMFVLCKDRKAAFFWDSV 335
           AF++I + +       F  P K CC   K G  CG     GKK++ LC D K+ FFWD+V
Sbjct: 283 AFVTIFKNKGVSGVSTFPDPFKACC-ATKRGTFCGDRSLSGKKLYTLCDDPKSFFFWDNV 342

Query: 336 HPTQQGWAAAFS 345
           H + QGW + FS
Sbjct: 343 HISDQGWRSVFS 348

BLAST of CmoCh03G012040 vs. ExPASy TrEMBL
Match: A0A6J1ECT1 (GDSL esterase/lipase At5g03610-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111433004 PE=3 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 1.5e-192
Identity = 332/359 (92.48%), Postives = 341/359 (94.99%), Query Frame = 0

Query: 1   MHFVLRLLFPLFLSISLFSGRVSAGGVYNH------HLRHRFRPTKLFVFGDSYTDTGNI 60
           M FVLRLLF L L ISLFSGR SAGGVYNH      HLRHRFRPTKLFVFGDSY DTGNI
Sbjct: 1   MDFVLRLLFSLLLLISLFSGRGSAGGVYNHHHHRRRHLRHRFRPTKLFVFGDSYADTGNI 60

Query: 61  LFPISPGLQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKET 120
           LFPISP LQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKET
Sbjct: 61  LFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKET 120

Query: 121 GVNFAFGGTGVFDTEYPLPNMTTQIDLFQQLRHGDSVFTNTDAQSSVALVSASGNDYSFY 180
           GVNFAFGGTGVFDTEYPLPNMTTQID FQQLRHGDS+FTNTDAQSSVALVSASGNDY+FY
Sbjct: 121 GVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSIFTNTDAQSSVALVSASGNDYAFY 180

Query: 181 FAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCN 240
           FAMNSSRQGLKPFIMSVV+QIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYK+CN
Sbjct: 181 FAMNSSRQGLKPFIMSVVSQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKQCN 240

Query: 241 ETVNSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGAFMSIIEGRKRYNNGEFKTPLK 300
           ET+NSLVQFHN LLKEAVKKLNQETKHSSKVF+VDVYGAFMSIIEGRKRYNNGEFKTPLK
Sbjct: 241 ETINSLVQFHNLLLKEAVKKLNQETKHSSKVFVVDVYGAFMSIIEGRKRYNNGEFKTPLK 300

Query: 301 PCCFGVKSGFDCGSVDGEGKKMFVLCKDRKAAFFWDSVHPTQQGWAAAFSAFKSFRNHF 354
           PCC+GV SGF CGSVDGEGKKMFVLC+D+KAAFFWDSVHPTQQGWAAA SAFKSFRNHF
Sbjct: 301 PCCYGVGSGFSCGSVDGEGKKMFVLCEDQKAAFFWDSVHPTQQGWAAAISAFKSFRNHF 359

BLAST of CmoCh03G012040 vs. ExPASy TrEMBL
Match: A0A6J1EIS2 (GDSL esterase/lipase At5g03610-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111433004 PE=3 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 1.5e-192
Identity = 332/359 (92.48%), Postives = 341/359 (94.99%), Query Frame = 0

Query: 1   MHFVLRLLFPLFLSISLFSGRVSAGGVYNH------HLRHRFRPTKLFVFGDSYTDTGNI 60
           M FVLRLLF L L ISLFSGR SAGGVYNH      HLRHRFRPTKLFVFGDSY DTGNI
Sbjct: 2   MDFVLRLLFSLLLLISLFSGRGSAGGVYNHHHHRRRHLRHRFRPTKLFVFGDSYADTGNI 61

Query: 61  LFPISPGLQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKET 120
           LFPISP LQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKET
Sbjct: 62  LFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKET 121

Query: 121 GVNFAFGGTGVFDTEYPLPNMTTQIDLFQQLRHGDSVFTNTDAQSSVALVSASGNDYSFY 180
           GVNFAFGGTGVFDTEYPLPNMTTQID FQQLRHGDS+FTNTDAQSSVALVSASGNDY+FY
Sbjct: 122 GVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSIFTNTDAQSSVALVSASGNDYAFY 181

Query: 181 FAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCN 240
           FAMNSSRQGLKPFIMSVV+QIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYK+CN
Sbjct: 182 FAMNSSRQGLKPFIMSVVSQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKQCN 241

Query: 241 ETVNSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGAFMSIIEGRKRYNNGEFKTPLK 300
           ET+NSLVQFHN LLKEAVKKLNQETKHSSKVF+VDVYGAFMSIIEGRKRYNNGEFKTPLK
Sbjct: 242 ETINSLVQFHNLLLKEAVKKLNQETKHSSKVFVVDVYGAFMSIIEGRKRYNNGEFKTPLK 301

Query: 301 PCCFGVKSGFDCGSVDGEGKKMFVLCKDRKAAFFWDSVHPTQQGWAAAFSAFKSFRNHF 354
           PCC+GV SGF CGSVDGEGKKMFVLC+D+KAAFFWDSVHPTQQGWAAA SAFKSFRNHF
Sbjct: 302 PCCYGVGSGFSCGSVDGEGKKMFVLCEDQKAAFFWDSVHPTQQGWAAAISAFKSFRNHF 360

BLAST of CmoCh03G012040 vs. ExPASy TrEMBL
Match: A0A6J1IRZ9 (GDSL esterase/lipase At5g03610-like OS=Cucurbita maxima OX=3661 GN=LOC111477899 PE=3 SV=1)

HSP 1 Score: 674.5 bits (1739), Expect = 2.4e-190
Identity = 326/356 (91.57%), Postives = 339/356 (95.22%), Query Frame = 0

Query: 1   MHFVLRLLFPLFLSISLFSGRVSAGGVYNHH---LRHRFRPTKLFVFGDSYTDTGNILFP 60
           M FVLRLLF L L ISLFSGR SAGGVYNHH   LRHRFRPTKLFVFGDSY DTGNILFP
Sbjct: 2   MDFVLRLLFSLLLLISLFSGRGSAGGVYNHHHQQLRHRFRPTKLFVFGDSYADTGNILFP 61

Query: 61  ISPGLQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKETGVN 120
           ISPG+QFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKETGVN
Sbjct: 62  ISPGMQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKETGVN 121

Query: 121 FAFGGTGVFDTEYPLPNMTTQIDLFQQLRHGDSVFTNTDAQSSVALVSASGNDYSFYFAM 180
           FAFGGTGVFDTEYPLPNMTTQID FQ LR GDS+ TNTDAQSSVALVSASGNDYSFYFAM
Sbjct: 122 FAFGGTGVFDTEYPLPNMTTQIDFFQHLRQGDSILTNTDAQSSVALVSASGNDYSFYFAM 181

Query: 181 NSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCNETV 240
           N+SRQGLKPFI+SVVNQI+VNLKRIGGLGVKKIAVTGLGPLGC PVFTAPSSYK+CNE++
Sbjct: 182 NTSRQGLKPFIISVVNQIMVNLKRIGGLGVKKIAVTGLGPLGCFPVFTAPSSYKQCNESI 241

Query: 241 NSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGAFMSIIEGRKRYNNGEFKTPLKPCC 300
           NSLVQFHNFLLKE VKKLNQETKHSSKVFIVDVYGAFMSII+GRKRYNNGEFKTPLKPCC
Sbjct: 242 NSLVQFHNFLLKEGVKKLNQETKHSSKVFIVDVYGAFMSIIQGRKRYNNGEFKTPLKPCC 301

Query: 301 FGVKSGFDCGSVDGEGKKMFVLCKDRKAAFFWDSVHPTQQGWAAAFSAFKSFRNHF 354
           +GV SGF CGSVDGEGK+MFVLC+DRKAAFFWDSVHPTQQGWAAAF+AFKSFRNHF
Sbjct: 302 YGVGSGFSCGSVDGEGKQMFVLCEDRKAAFFWDSVHPTQQGWAAAFAAFKSFRNHF 357

BLAST of CmoCh03G012040 vs. ExPASy TrEMBL
Match: A0A6J1IMH1 (GDSL esterase/lipase At5g03610-like OS=Cucurbita maxima OX=3661 GN=LOC111478378 PE=3 SV=1)

HSP 1 Score: 672.5 bits (1734), Expect = 9.3e-190
Identity = 326/356 (91.57%), Postives = 338/356 (94.94%), Query Frame = 0

Query: 1   MHFVLRLLFPLFLSISLFSGRVSAGGVYNHH---LRHRFRPTKLFVFGDSYTDTGNILFP 60
           M FVLRLLF L L ISLFSGR SAGGVYNHH   LRHRFRPTKLFVFGDSY DTG ILFP
Sbjct: 2   MDFVLRLLFSLLLLISLFSGRGSAGGVYNHHDQQLRHRFRPTKLFVFGDSYADTGIILFP 61

Query: 61  ISPGLQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKETGVN 120
           ISPG+QFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRR KETGVN
Sbjct: 62  ISPGMQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRLKETGVN 121

Query: 121 FAFGGTGVFDTEYPLPNMTTQIDLFQQLRHGDSVFTNTDAQSSVALVSASGNDYSFYFAM 180
           FAFGGTGVFDTEYPLPNMTTQID FQ LR GDS+FTNTDAQSSVALVSASGNDYSFYFAM
Sbjct: 122 FAFGGTGVFDTEYPLPNMTTQIDFFQHLRQGDSIFTNTDAQSSVALVSASGNDYSFYFAM 181

Query: 181 NSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCNETV 240
           N+SRQGLKPFI+SVVNQI+VNLKRIGGLGVKKIAVTGLGPLGC PVFTAPSSYK+CNE++
Sbjct: 182 NTSRQGLKPFIISVVNQIMVNLKRIGGLGVKKIAVTGLGPLGCFPVFTAPSSYKQCNESI 241

Query: 241 NSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGAFMSIIEGRKRYNNGEFKTPLKPCC 300
           NSLVQFHNFLLKE VKKLNQETKHSSKVFIVDVYGAFMSII+GRKRYNNGEFKTPLKPCC
Sbjct: 242 NSLVQFHNFLLKEGVKKLNQETKHSSKVFIVDVYGAFMSIIQGRKRYNNGEFKTPLKPCC 301

Query: 301 FGVKSGFDCGSVDGEGKKMFVLCKDRKAAFFWDSVHPTQQGWAAAFSAFKSFRNHF 354
           +GV SGF CGSVDGEGKKMFVLC+DRKAAFFWDSVHPTQQGWAAAF+AFKSFRNHF
Sbjct: 302 YGVGSGFSCGSVDGEGKKMFVLCEDRKAAFFWDSVHPTQQGWAAAFAAFKSFRNHF 357

BLAST of CmoCh03G012040 vs. ExPASy TrEMBL
Match: A0A6J1EC94 (GDSL esterase/lipase At5g03610-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111433004 PE=3 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 4.3e-171
Identity = 295/356 (82.87%), Postives = 316/356 (88.76%), Query Frame = 0

Query: 1   MHFVLRLLFPLFLSISLFSGRVSAGGVYN---HHLRHRFRPTKLFVFGDSYTDTGNILFP 60
           M FVLRLLF L L ISLFSGRVSAGGVYN   HH RHR RPTKLFVFGDSY DTGNIL P
Sbjct: 1   MDFVLRLLFSLLLLISLFSGRVSAGGVYNHHHHHQRHRLRPTKLFVFGDSYVDTGNILIP 60

Query: 61  ISPGLQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKETGVN 120
           +S   QFPYGITFPGKP GRFSDGRVLTDFAAR+VGLKSPIPF+V+NRVGLRRFKETGVN
Sbjct: 61  LSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPFTVQNRVGLRRFKETGVN 120

Query: 121 FAFGGTGVFDTEYPLPNMTTQIDLFQQLRHGDSVFTNTDAQSSVALVSASGNDYSFYFAM 180
           FAFGGTGVFDT +P PNMTTQID FQQL+HG+S+ T TD Q S ALVS SGNDYS     
Sbjct: 121 FAFGGTGVFDTSFPFPNMTTQIDFFQQLQHGESLSTKTDNQFSAALVSVSGNDYSLDIIT 180

Query: 181 NSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCNETV 240
           N  +QGLKPFI +VVNQIIV+LKRIG LGVKKIAVTGLGPLGCLP FTAPSS+KKCNET+
Sbjct: 181 NGPKQGLKPFINTVVNQIIVDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNETI 240

Query: 241 NSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGAFMSIIEGRKRYNNGEFKTPLKPCC 300
           NS V+FHNFLLK+AVKKLNQETKHSSKVF++D+YGAFMSIIEG KRYNNG FKTPLKPCC
Sbjct: 241 NSFVKFHNFLLKKAVKKLNQETKHSSKVFVLDMYGAFMSIIEGEKRYNNGVFKTPLKPCC 300

Query: 301 FGVKSGFDCGSVDGEGKKMFVLCKDRKAAFFWDSVHPTQQGWAAAFSAFKSFRNHF 354
           FGVKSGFDCGSVDGEGKKMFVLCKD K AF+WD+VHPTQQGWAAAFSA K+FRNHF
Sbjct: 301 FGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAFSALKAFRNHF 356

BLAST of CmoCh03G012040 vs. NCBI nr
Match: KAG7034546.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 716.5 bits (1848), Expect = 1.2e-202
Identity = 349/356 (98.03%), Postives = 351/356 (98.60%), Query Frame = 0

Query: 1   MHFVLRLLFPLFLSISLFSGRVSAGGVYN---HHLRHRFRPTKLFVFGDSYTDTGNILFP 60
           MHFVLR LFPLFLSISLFSGRVSAGGVYN   HHLRHRFRPTKLFVFGDSYTDTGNILFP
Sbjct: 1   MHFVLRPLFPLFLSISLFSGRVSAGGVYNHHHHHLRHRFRPTKLFVFGDSYTDTGNILFP 60

Query: 61  ISPGLQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKETGVN 120
           ISPGLQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKETGVN
Sbjct: 61  ISPGLQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKETGVN 120

Query: 121 FAFGGTGVFDTEYPLPNMTTQIDLFQQLRHGDSVFTNTDAQSSVALVSASGNDYSFYFAM 180
           FAFGGTGVFDTEYPLPNMTTQID FQQLRHGDSVFTNTDAQSSVALVSASGNDYSFYFAM
Sbjct: 121 FAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSVFTNTDAQSSVALVSASGNDYSFYFAM 180

Query: 181 NSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCNETV 240
           NSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCNET+
Sbjct: 181 NSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCNETI 240

Query: 241 NSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGAFMSIIEGRKRYNNGEFKTPLKPCC 300
           NSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGAFMSII+GRKRYNNGEFKTPLKPCC
Sbjct: 241 NSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGAFMSIIQGRKRYNNGEFKTPLKPCC 300

Query: 301 FGVKSGFDCGSVDGEGKKMFVLCKDRKAAFFWDSVHPTQQGWAAAFSAFKSFRNHF 354
           FGVKSGFDCGSVDGEGKKMFVLCKDRKAAFFWDSVHPTQQGWAAAFSAFKSFRNHF
Sbjct: 301 FGVKSGFDCGSVDGEGKKMFVLCKDRKAAFFWDSVHPTQQGWAAAFSAFKSFRNHF 356

BLAST of CmoCh03G012040 vs. NCBI nr
Match: KAG6604396.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 716.1 bits (1847), Expect = 1.5e-202
Identity = 350/356 (98.31%), Postives = 350/356 (98.31%), Query Frame = 0

Query: 1   MHFVLRLLFPLFLSISLFSGRVSAGGVYN---HHLRHRFRPTKLFVFGDSYTDTGNILFP 60
           MHFVLR LFPLFLSISLFSGRVSAGGVYN   HHLRHRFRPTKLFVFGDSYTDTGNILFP
Sbjct: 1   MHFVLRPLFPLFLSISLFSGRVSAGGVYNHHHHHLRHRFRPTKLFVFGDSYTDTGNILFP 60

Query: 61  ISPGLQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKETGVN 120
           ISPGLQFPYGITFPGKP GRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKETGVN
Sbjct: 61  ISPGLQFPYGITFPGKPTGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKETGVN 120

Query: 121 FAFGGTGVFDTEYPLPNMTTQIDLFQQLRHGDSVFTNTDAQSSVALVSASGNDYSFYFAM 180
           FAFGGTGVFDTEYPLPNMTTQID FQQLRHGDSVFTNTDAQSSVALVSASGNDYSFYFAM
Sbjct: 121 FAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSVFTNTDAQSSVALVSASGNDYSFYFAM 180

Query: 181 NSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCNETV 240
           NSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCNETV
Sbjct: 181 NSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCNETV 240

Query: 241 NSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGAFMSIIEGRKRYNNGEFKTPLKPCC 300
           NSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGAFMSIIEGRKRYNNGEFKTPLKPCC
Sbjct: 241 NSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGAFMSIIEGRKRYNNGEFKTPLKPCC 300

Query: 301 FGVKSGFDCGSVDGEGKKMFVLCKDRKAAFFWDSVHPTQQGWAAAFSAFKSFRNHF 354
           FGVKSGFDCGSVDGEGKKMFVLCKDRKAAFFWDSVHPTQQGWAAAFSAFKSFRNHF
Sbjct: 301 FGVKSGFDCGSVDGEGKKMFVLCKDRKAAFFWDSVHPTQQGWAAAFSAFKSFRNHF 356

BLAST of CmoCh03G012040 vs. NCBI nr
Match: KAG6604360.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 697.2 bits (1798), Expect = 7.3e-197
Identity = 340/356 (95.51%), Postives = 344/356 (96.63%), Query Frame = 0

Query: 1   MHFVLRLLFPLFLSISLFSGRVSAGGVYN---HHLRHRFRPTKLFVFGDSYTDTGNILFP 60
           M FVLRLLF L L ISLFSGR SAGGVYN   HHLRHRFRPTKLFVFGDSY DTGNILFP
Sbjct: 2   MDFVLRLLFSLLLLISLFSGRGSAGGVYNHHHHHLRHRFRPTKLFVFGDSYADTGNILFP 61

Query: 61  ISPGLQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKETGVN 120
           ISP LQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIP+SVR+RVGLRRFKETGVN
Sbjct: 62  ISPALQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPYSVRSRVGLRRFKETGVN 121

Query: 121 FAFGGTGVFDTEYPLPNMTTQIDLFQQLRHGDSVFTNTDAQSSVALVSASGNDYSFYFAM 180
           FAFGGTGVFDTEYPLPNMTTQID FQQLRHGDS+FTNTD QSSVALVSASGNDYSFYFAM
Sbjct: 122 FAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSIFTNTDVQSSVALVSASGNDYSFYFAM 181

Query: 181 NSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCNETV 240
           NSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCNET+
Sbjct: 182 NSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCNETI 241

Query: 241 NSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGAFMSIIEGRKRYNNGEFKTPLKPCC 300
           NSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGAFMSIIEGRKRYNNGEFKTPLKPCC
Sbjct: 242 NSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGAFMSIIEGRKRYNNGEFKTPLKPCC 301

Query: 301 FGVKSGFDCGSVDGEGKKMFVLCKDRKAAFFWDSVHPTQQGWAAAFSAFKSFRNHF 354
           FGVKSGFDCGSVDGEGKKMFVLCKDRKAAFFWDSVHPTQQGWAAAFSAFKSFRNHF
Sbjct: 302 FGVKSGFDCGSVDGEGKKMFVLCKDRKAAFFWDSVHPTQQGWAAAFSAFKSFRNHF 357

BLAST of CmoCh03G012040 vs. NCBI nr
Match: XP_022925651.1 (GDSL esterase/lipase At5g03610-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 681.8 bits (1758), Expect = 3.2e-192
Identity = 332/359 (92.48%), Postives = 341/359 (94.99%), Query Frame = 0

Query: 1   MHFVLRLLFPLFLSISLFSGRVSAGGVYNH------HLRHRFRPTKLFVFGDSYTDTGNI 60
           M FVLRLLF L L ISLFSGR SAGGVYNH      HLRHRFRPTKLFVFGDSY DTGNI
Sbjct: 1   MDFVLRLLFSLLLLISLFSGRGSAGGVYNHHHHRRRHLRHRFRPTKLFVFGDSYADTGNI 60

Query: 61  LFPISPGLQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKET 120
           LFPISP LQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKET
Sbjct: 61  LFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKET 120

Query: 121 GVNFAFGGTGVFDTEYPLPNMTTQIDLFQQLRHGDSVFTNTDAQSSVALVSASGNDYSFY 180
           GVNFAFGGTGVFDTEYPLPNMTTQID FQQLRHGDS+FTNTDAQSSVALVSASGNDY+FY
Sbjct: 121 GVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSIFTNTDAQSSVALVSASGNDYAFY 180

Query: 181 FAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCN 240
           FAMNSSRQGLKPFIMSVV+QIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYK+CN
Sbjct: 181 FAMNSSRQGLKPFIMSVVSQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKQCN 240

Query: 241 ETVNSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGAFMSIIEGRKRYNNGEFKTPLK 300
           ET+NSLVQFHN LLKEAVKKLNQETKHSSKVF+VDVYGAFMSIIEGRKRYNNGEFKTPLK
Sbjct: 241 ETINSLVQFHNLLLKEAVKKLNQETKHSSKVFVVDVYGAFMSIIEGRKRYNNGEFKTPLK 300

Query: 301 PCCFGVKSGFDCGSVDGEGKKMFVLCKDRKAAFFWDSVHPTQQGWAAAFSAFKSFRNHF 354
           PCC+GV SGF CGSVDGEGKKMFVLC+D+KAAFFWDSVHPTQQGWAAA SAFKSFRNHF
Sbjct: 301 PCCYGVGSGFSCGSVDGEGKKMFVLCEDQKAAFFWDSVHPTQQGWAAAISAFKSFRNHF 359

BLAST of CmoCh03G012040 vs. NCBI nr
Match: XP_022925650.1 (GDSL esterase/lipase At5g03610-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 681.8 bits (1758), Expect = 3.2e-192
Identity = 332/359 (92.48%), Postives = 341/359 (94.99%), Query Frame = 0

Query: 1   MHFVLRLLFPLFLSISLFSGRVSAGGVYNH------HLRHRFRPTKLFVFGDSYTDTGNI 60
           M FVLRLLF L L ISLFSGR SAGGVYNH      HLRHRFRPTKLFVFGDSY DTGNI
Sbjct: 2   MDFVLRLLFSLLLLISLFSGRGSAGGVYNHHHHRRRHLRHRFRPTKLFVFGDSYADTGNI 61

Query: 61  LFPISPGLQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKET 120
           LFPISP LQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKET
Sbjct: 62  LFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKET 121

Query: 121 GVNFAFGGTGVFDTEYPLPNMTTQIDLFQQLRHGDSVFTNTDAQSSVALVSASGNDYSFY 180
           GVNFAFGGTGVFDTEYPLPNMTTQID FQQLRHGDS+FTNTDAQSSVALVSASGNDY+FY
Sbjct: 122 GVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSIFTNTDAQSSVALVSASGNDYAFY 181

Query: 181 FAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCN 240
           FAMNSSRQGLKPFIMSVV+QIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYK+CN
Sbjct: 182 FAMNSSRQGLKPFIMSVVSQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKQCN 241

Query: 241 ETVNSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGAFMSIIEGRKRYNNGEFKTPLK 300
           ET+NSLVQFHN LLKEAVKKLNQETKHSSKVF+VDVYGAFMSIIEGRKRYNNGEFKTPLK
Sbjct: 242 ETINSLVQFHNLLLKEAVKKLNQETKHSSKVFVVDVYGAFMSIIEGRKRYNNGEFKTPLK 301

Query: 301 PCCFGVKSGFDCGSVDGEGKKMFVLCKDRKAAFFWDSVHPTQQGWAAAFSAFKSFRNHF 354
           PCC+GV SGF CGSVDGEGKKMFVLC+D+KAAFFWDSVHPTQQGWAAA SAFKSFRNHF
Sbjct: 302 PCCYGVGSGFSCGSVDGEGKKMFVLCEDQKAAFFWDSVHPTQQGWAAAISAFKSFRNHF 360

BLAST of CmoCh03G012040 vs. TAIR 10
Match: AT5G03610.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 362.8 bits (930), Expect = 3.0e-100
Identity = 183/352 (51.99%), Postives = 243/352 (69.03%), Query Frame = 0

Query: 1   MHFVLRLLFPL--FLSISLFSGRVSA--GGVYNHHLRHRFRPTKLFVFGDSYTDTGNILF 60
           M  +++L F L  FL  SL  G ++   G   NHHL + FRPTKLFVFGDSY DTGNI  
Sbjct: 1   MDSLIKLFFCLFIFLCTSLLFGEINGVEGSNQNHHL-YPFRPTKLFVFGDSYADTGNIKK 60

Query: 61  PISPGLQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKETGV 120
             S   +FPYGITFPGKP GRFSDGRV TDF A+ VG+KSPIP+  ++  G +R  + G+
Sbjct: 61  AFSSSWKFPYGITFPGKPAGRFSDGRVATDFLAKFVGIKSPIPYFWKDYAGKKRL-QYGM 120

Query: 121 NFAFGGTGVFDTEYPLPNMTTQIDLFQQLRHGDSVFTNTDAQSSVALVSASGNDYSFYFA 180
           NFA+GGTGVF+T+ PLPNMTTQID+FQ +     ++   +  SSVALVS +GNDYS + A
Sbjct: 121 NFAYGGTGVFNTQTPLPNMTTQIDIFQNILTTGDIYYPPELTSSVALVSVAGNDYSNFIA 180

Query: 181 MNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCNET 240
           +N        FI  VV+Q  VNL+RI  LGVKKIAV  L PLGCLP FT  +S+++CNET
Sbjct: 181 LNRPASEFPAFIKQVVDQTEVNLRRIHALGVKKIAVPSLQPLGCLPPFTFVTSFQRCNET 240

Query: 241 VNSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGAFMSIIEGR-KRYNNGEFKTPLKP 300
            N+LV  HN LL++ V KLN ETK S+ + I+D+Y AF+++ + +     +  F++PLKP
Sbjct: 241 QNALVNLHNNLLQQVVAKLNNETKQSTFI-ILDLYNAFLTVFKNKGSNPGSTRFESPLKP 300

Query: 301 CCFGVKSGFDCGSVDGEGKKMFVLCKDRKAAFFWDSVHPTQQGWAAAFSAFK 348
           CC GV   ++CGSVD +G K +++C + K AFFWD +HPT++GW + +S  +
Sbjct: 301 CCVGVSREYNCGSVDEKGVKKYIVCDNPKTAFFWDGLHPTEEGWRSVYSVLR 349

BLAST of CmoCh03G012040 vs. TAIR 10
Match: AT3G09930.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 323.6 bits (828), Expect = 2.0e-88
Identity = 164/342 (47.95%), Postives = 226/342 (66.08%), Query Frame = 0

Query: 6   RLLFPLFLSISLFSGRVSAGGVYNHHLR-HRFRPTKLFVFGDSYTDTGNILFPISPGLQF 65
           +LL  LFL  S  S  + A   Y  H    R RP +LFVFGDSY DTGNI   +S   + 
Sbjct: 5   KLLISLFL-FSFSSFFLGAESDYPQHSNLKRLRPNRLFVFGDSYADTGNIRKSLSDSWKI 64

Query: 66  PYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKETGVNFAFGGTG 125
           PYGITFP KP GRFSDGRV TDF AR++G+KSPIP++ ++  G  R    G+N+A+GGTG
Sbjct: 65  PYGITFPQKPSGRFSDGRVATDFLARYLGIKSPIPYTWKDYAGKERLL-YGMNYAYGGTG 124

Query: 126 VFDT-EYPLPNMTTQIDLFQQLRHGDSVFTNTDAQSSVALVSASGNDYSFYFAMNSSRQG 185
           VF T + PLPNMTTQID FQ++    ++++ +D  SS+ALVS +GNDY+ + A+      
Sbjct: 125 VFKTKDNPLPNMTTQIDYFQRVLAAGNIYSPSDLPSSLALVSVAGNDYATFLALKRPLTE 184

Query: 186 LKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCNETVNSLVQF 245
           L  F+  VV+QI VN  RI  LGV KI +  + PLGCLP  T  +S+++CN T N+    
Sbjct: 185 LPAFMKQVVDQIAVNAMRIHKLGVNKIVIPSMQPLGCLPSITVFNSFQRCNATDNASTNL 244

Query: 246 HNFLLKEAVKKLNQETKHSSKVFIVDVYGAFMSIIEGR-KRYNNGEFKTPLKPCCFGVKS 305
           HN+LL +A+ +LN ETK S+ V ++D Y AF+++ + +        F  PLKPCC GV S
Sbjct: 245 HNYLLHKAIARLNNETKPSTFV-VLDHYNAFLTVFKNKGPEPGVSRFGNPLKPCCVGVNS 304

Query: 306 GFDCGSVDGEGKKMFVLCKDRKAAFFWDSVHPTQQGWAAAFS 345
            +DC +VD +G+K +++C+D KAAFFWD  HP+++GW + +S
Sbjct: 305 SYDCSNVDEKGEKKYIICEDPKAAFFWDIFHPSEEGWRSVYS 343

BLAST of CmoCh03G012040 vs. TAIR 10
Match: AT5G03600.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 255.0 bits (650), Expect = 8.9e-68
Identity = 141/322 (43.79%), Postives = 195/322 (60.56%), Query Frame = 0

Query: 29  NHHLRHRFRPTKLFVFGDSYTDTGNILFPISPGLQFPYGITFPGKPIGRFSDGRVLTDFA 88
           N  LR R  P KLFVFGDSY DTGN     +     PYGITFPGKP GR+ DG + TDF 
Sbjct: 4   NQLLRPRKVP-KLFVFGDSYADTGNTKRD-TEAWAIPYGITFPGKPSGRYCDGLIATDFL 63

Query: 89  ARHVGLKSPIPFSVRNR-VGLRRFKETGVNFAFGGTGVFDT--EYPLPNMTTQIDLFQQL 148
            + +G +SP  +    R  GL+R    G+NFAFGG+ + D+    P PN+T Q++    L
Sbjct: 64  EKVLGAESPYLYRTHGRDKGLKR----GMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDL 123

Query: 149 RHGDSVFTNTDAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGL 208
                V+ +    S V+L+S +G DY +Y   N    GLK  +  VV+ + VN+  +GGL
Sbjct: 124 VLAGRVYGDI-TPSDVSLISYAGGDYIYYIDQNRPAAGLKALVEKVVDNLRVNMIVLGGL 183

Query: 209 GVKKIAVTGLGPLGCLPVFTAPSSYKKCNETVNSLVQFHNFLLKEAVKKLNQET---KHS 268
             KKIAVT L P+GCLP +T+ SS+K CNE+ ++LV+ HN LLK+ V KLN+++   K  
Sbjct: 184 LFKKIAVTSLQPIGCLPSYTSASSFKSCNESQSALVELHNKLLKKVVAKLNEQSRVMKKE 243

Query: 269 SKVFIVDVYGAFMSIIEGRKRYNNGEFKTPLKPCCFGVKSGFDCGSVDGEGKKMFVLCKD 328
              FI+D++ AFM+++   K   +  FK P+K CC G      CG    +G K++ LC D
Sbjct: 244 QHFFIIDIHNAFMTVM---KNKGSKRFKNPMKSCCEGY-----CGR-SSDGGKLYTLCDD 303

Query: 329 RKAAFFWDSVHPTQQGWAAAFS 345
            K+ FFWD+VHPTQ+GW + +S
Sbjct: 304 PKSFFFWDAVHPTQEGWRSIYS 309

BLAST of CmoCh03G012040 vs. TAIR 10
Match: AT2G36325.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 248.1 bits (632), Expect = 1.1e-65
Identity = 137/312 (43.91%), Postives = 186/312 (59.62%), Query Frame = 0

Query: 36  FRPTKLFVFGDSYTDTGNILFPISPGLQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLK 95
           F+PTKLFVFGDSY DTGN  F I P  +FP GITFPG P GRFSDGRV TD+ A+++G++
Sbjct: 43  FKPTKLFVFGDSYADTGNTPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYLAKYIGVR 102

Query: 96  SPIPFSVRNRVGLRRFKETGVNFAFGGTGVFDTEYPL-PNMTTQIDLFQQLRHGDSVFTN 155
           +PI +        R   + G+NFA+GG G F+T + L P  + QID F+QL    +V++ 
Sbjct: 103 TPITYKWGKYGRPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQLLM-RNVYSP 162

Query: 156 TDAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTG 215
            D  SSVA  S  GNDY  Y   N S +G       VV QI++++KRI  LGV+K+ V  
Sbjct: 163 ADLNSSVAFFSIIGNDYLTYDRRNGSEEGRSALTRKVVKQILLDVKRIKDLGVRKVLVAL 222

Query: 216 LGPLGCLPVFTAPSSYKKCNETVNSLVQFHNFLLKEAVKKLN-QETKHSSKVFI-VDVYG 275
             P  CLP    P   K C +T ++    HN LL++ + KLN +E  ++ K F+ +D+Y 
Sbjct: 223 SPPQKCLPKLVTP---KGC-DTNDTSTYLHNSLLRKGLIKLNDKEINNNDKSFMTLDLYN 282

Query: 276 AFMSIIEGRKRYNNGEFKTPLKPCCFGVKSGFDCGSVDGEGKKMFVLCKDRKAAFFWDSV 335
           AF++I + +       F  P K CC   K G  CG     GKK++ LC D K+ FFWD+V
Sbjct: 283 AFVTIFKNKGVSGVSTFPDPFKACC-ATKRGTFCGDRSLSGKKLYTLCDDPKSFFFWDNV 342

Query: 336 HPTQQGWAAAFS 345
           H + QGW + FS
Sbjct: 343 HISDQGWRSVFS 348

BLAST of CmoCh03G012040 vs. TAIR 10
Match: AT5G03590.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 151.8 bits (382), Expect = 1.1e-36
Identity = 99/292 (33.90%), Postives = 134/292 (45.89%), Query Frame = 0

Query: 57  PISPGLQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKETGV 116
           P +   + PYGITFPGKP GR+SDG   TDF    V   SP+  S               
Sbjct: 3   PTALSWKLPYGITFPGKPSGRYSDGLTATDFLGSEV-FDSPVDRS--------------- 62

Query: 117 NFAFGGTGVFDTEYPLPNMTTQIDLFQQLRHGDSVFT-NTDAQSSVALVSASGNDYSFYF 176
                           PN++TQ+     L     V+T + D  SS AL+S SG DY  + 
Sbjct: 63  ----------------PNISTQVGFLVNLALARRVYTIDGDLASSYALLSYSGTDYYGFI 122

Query: 177 AMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCNE 236
             N +      F+  +V  I                                     CNE
Sbjct: 123 DQNPNMAAYPAFVEFIVEDI------------------------------------HCNE 182

Query: 237 TVNSLVQFHNFLLKEAVKKLNQETKHSSK---VFIVDVYGAFMSIIEGRKRYNNGEFKTP 296
           + + LV+ HN  LK+AV KLN+E K  +K     IVD++ AFM+I+E   +  N  FK+P
Sbjct: 183 SYSDLVRLHNESLKKAVAKLNKEDKFRTKGDRFVIVDLHKAFMTILE---KKGNKRFKSP 218

Query: 297 LKPCCFGVKSGFDCGSVDGEGKKMFVLCKDRKAAFFWDSVHPTQQGWAAAFS 345
           LKPCC G     DC  +D +G K + LC D K+AFFWD ++PTQ+GW + +S
Sbjct: 243 LKPCCEG-----DCARMDMKGAKKYTLCNDPKSAFFWDEINPTQEGWRSIYS 218

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LZS74.3e-9951.99GDSL esterase/lipase At5g03610 OS=Arabidopsis thaliana OX=3702 GN=At5g03610 PE=2... [more]
Q9SF942.9e-8747.95GDSL esterase/lipase At3g09930 OS=Arabidopsis thaliana OX=3702 GN=At3g09930 PE=2... [more]
Q9LZS91.3e-6843.59GDSL esterase/lipase At5g03590 OS=Arabidopsis thaliana OX=3702 GN=At5g03590 PE=3... [more]
Q9LZS81.3e-6643.79GDSL esterase/lipase At5g03600 OS=Arabidopsis thaliana OX=3702 GN=At5g03600 PE=3... [more]
Q8RWJ41.5e-6443.91GDSL esterase/lipase At2g36325 OS=Arabidopsis thaliana OX=3702 GN=At2g36325 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1ECT11.5e-19292.48GDSL esterase/lipase At5g03610-like isoform X2 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1EIS21.5e-19292.48GDSL esterase/lipase At5g03610-like isoform X1 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1IRZ92.4e-19091.57GDSL esterase/lipase At5g03610-like OS=Cucurbita maxima OX=3661 GN=LOC111477899 ... [more]
A0A6J1IMH19.3e-19091.57GDSL esterase/lipase At5g03610-like OS=Cucurbita maxima OX=3661 GN=LOC111478378 ... [more]
A0A6J1EC944.3e-17182.87GDSL esterase/lipase At5g03610-like isoform X3 OS=Cucurbita moschata OX=3662 GN=... [more]
Match NameE-valueIdentityDescription
KAG7034546.11.2e-20298.03GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6604396.11.5e-20298.31GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG6604360.17.3e-19795.51GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022925651.13.2e-19292.48GDSL esterase/lipase At5g03610-like isoform X2 [Cucurbita moschata][more]
XP_022925650.13.2e-19292.48GDSL esterase/lipase At5g03610-like isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G03610.13.0e-10051.99GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT3G09930.12.0e-8847.95GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT5G03600.18.9e-6843.79SGNH hydrolase-type esterase superfamily protein [more]
AT2G36325.11.1e-6543.91GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT5G03590.11.1e-3633.90GDSL-like Lipase/Acylhydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 41..345
e-value: 2.9E-29
score: 103.1
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 28..351
e-value: 3.0E-59
score: 203.0
NoneNo IPR availablePANTHERPTHR46020OSJNBB0059K02.9 PROTEINcoord: 28..348
NoneNo IPR availablePANTHERPTHR46020:SF4OSJNBB0059K02.9 PROTEINcoord: 28..348
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 38..346
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 41..346
e-value: 3.5025E-89
score: 268.715

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh03G012040.1CmoCh03G012040.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016788 hydrolase activity, acting on ester bonds