CmoCh03G012030 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh03G012030
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Histidine--tRNA ligase
Location	Cmo_Chr03: 9377527 .. 9382758 (+)
RNA-Seq Expression	CmoCh03G012030
Synteny	CmoCh03G012030

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTCAGAAGGCGCCAATGAGCAATTGACTGTGCTGCGTCTCTGAACTGTCGACCACCATGGCTGCGAAGCCTTCATCGCTCCTTATCTTCCAACCTCGCCTCAATCTTCGTTTGAAGCCCTTCATTTTCTCTTCTTCGTCACTCGCTTTCAGTATCATTCTTAGAATCCCCTTTCCGAAGGCCTCATTCTTCGGTAACCGCAAATTTCATTCTTCTCTCAGCGCCGTCTCTTCCGCCCAGTCTACCGGTGACGGCGGAAATGGTGGCGGCAGGTCGGGGGCGCTATCTCCGACGCTGGTCACTGAAGAATTGCAGAAGATCGATGTTAACCCTCCCAAAGGAACCAGGGATTTTCCGCCGGAAGATATGCGACTCCGCAACTGGCTATTCAATAATTTCAGAGAGGTTTCGTATCCGAATTGTAGTTTGCTTTGTGGTTTTTGTGTCTTTGGTCTTTAGTTTTTGTTGCTGAATAGGTTTCACGAGTATATGGATTTGAGGAGGTTGATTTTCCGGTCCTTGAGTCGGAGGCGCTGTTCATTAGGAAAGCAGGTGAAGAAATAAGAGACCAGGTTGGTGTCGGTGTAATTTTTGTGTTGGTTCCTTCCTTAACTAATTTTGGACACTAATTTCTTGAATTTAGCCAAAATCTTGATCATACAAGAAAGGAAGAAGAATGGCTATATCCTGTAATTACATAAAATCCAGAATATACAGAATTTTGGCCTTTTACTTTATTCACGTTTCAAATTTTTTTGAGCTTGTATTCTGTGCACCATCTGCCTTCTTCCTGCCCCCACCCTGTTCATATGTTGCAAACAGTTGCCTCTTCATATTAGAGCATATTGGTAGAAGAATATTGGAATAAAATCTAATTGATGTTGGAATTTAATCTTGTGTAATCGTTGGTTAATTTCGTGCTCTTTGTTTTTGTAGCTCTATTGTTTTGAAGATCGGGGTAATCGTCGAGTTGCTTTGAGACCTGAGCTTACTCCTTCTTTGGCAAGATTAGTGATCCAGAAAGGGTACTTTGCATTATTAGATGCACTTTGTTTTCATTTATCTTGGAACGTGTTGGTATATTCTATCACTTTTCTTGTAAATAAATAAATAAAAAAAATTGAAATCATGTGTGTGAAAAATTAGTATCTGTCTATTTATTTATTTTTAATTATCTTTGTATCCAGGTGCAGATGTCAGAATTTGATCTCCATCTTTTTTTCTGTCCAGAAAATCTTTGTCCCTTCCACTGAAATGGTTTGCTATAGGGCAATGCTGGCGGTATGAGAGAATGACTAGAGGTCGGCGTCGTGAGCATTACCAATGGAATATGGATATCATTGGTGTACCCGAGGTTACGGTATGTGAAGTTGTAGACTTGCTAGCAGAACATTGGGGTTGGATTGAGTATTACTTTTCTAGTCATAATGAGCTTGTTTTGTGATATAAGAAATCAACAATCATGAAACTTTCTATCCCTTCAACATGGGATGTGTGTCCCTTCATTTCATGCCTTCAAGGGTCTGCACTGTGAGATGCATGTGTTCTATTTCATCTTCATGTTGTGTAAACTAAACAAAAAGAAAAAAGAAAAGAACAATCGTTTTAGGGAAGCAAAGTATGTAATGTGTGTGATGGCATGAAGAAGTCCCAGGTATCAGTTGAAAAAGTTGCAGCGGGTTATCAAAGTTCAGCAGAAGGCATCAGTTTTTTTTTACTCTTTTGAAGAAAAGAGTTTTTGCATGATTAGGCAGCTTTGAAGCTGTGAATTTCTTGTGGTTTCCAATTTGTTGCTTCCAGCTTGTAGTGAATATAGTTCATGCGTTCTCTAAAGATGCATGATGCAAAAAATGGTATTTTCTTTTTCTGGTGCAGCAAAGAAGATAATAGCAAGCACTTGTAAAGTCGGATTTCTGTCATTTTTTCTATGGATATTTCTGTTGGTAAATCTTTAGTCTTCTCAAGTGTTTGTGGGTTCTGAGCCTGTAATTTAGTTTATCTCGTCCTTTGTTAGTTGCTGCTTTTCATTTCTTTACCTTAGGGAAAAAAATGTGAACCCATCTAGCCATGTTGACATGTTCTAGTTGTAAACTCTTTTGTACCTGCCTTGTTTGAACAGGGTACCTTTTTATATATATACAAATATGCATGTGTGCACATTTTATTTAAGCTCATATATATATATATATTTTGTGTAGGCCGAAGCAGAGCTTCTATCTTCTATTGTTGCCTTTTTCAAACGAATCGGACTTACAGCAGCAGATGTTGGATTTAAGGTTTCCAGTCGAAAGGTATTTTTTTCTGCATCTTTTGTGATTATTCCAATAAATTTAGTTATTGCTTTATTAATTATTGAACATTCAATCATGAATCGAAGACTTTAATTGTTTTAGTTTCTGTAAATGCCTTATGTTGTAAGTTTGATGGTAGGTTTTGCAAGAAGTATTAAAATGCTACGGAATACCTGAAAATATGTTTGGCAAGGTTTGCATCATCATAGACAAGGTTGGTTATCTATGCCTTCAAGTTCCATAAATATTTACATTTTTGTGCACGTTCTTGACGCACGTCTCGATGTAATAATTTCTTCTCTGTCTCTGTTTCCTTCTGGCTATTAATATTAACAACAGATAGAAAAAATTCCATTGGATGAGATGAAGAAGGAACTGAAGGTTGTTGGTGTATCAGAGGATGCTATCGAGGAGATGTTGCAAGTTCTTTCAGTAAAGTCTTTGACAAAGTTAGAAGGTTATTTTCTGCAAATGGTTAAGTCTTTAGCAAATTAGTTGAAATGCATGTGATAATTATGAAAAATGAAAATCTGATGAAGACATTGAAATTAATTGAAGCGTGATTGTTATCCTTGCTTAGAGATACTTGGAGGTGGTGGTGAAGCCATGTCTGATCTGAAGCAGCTATTTTCTCTTGCTGAGAAGTTTGGTTATTCTGATTGGATCCAATTCGACGCGTCAATTGTCCGCGGCCTTGCTTATTACACTGGTATTGTTTTTGAGGTTGGTATTTGATATTCACTCCTTGTTGATTTGACTTGTCTTCATGCTGTAGAATAGGTCAAACATATATTATATGTCATTAACTGACTGAATGCCACGTTCGACACGTTAAACTACAGTGAGCTTCTAGATTCTCATGTAAATGGAATAATGTCGGCAAAGCTTCACACTAAATGGCTACAATCCCCATGTATAGTCTAATATTAAAAATTATATGAAATTTTGAATATCAAATAATTTCATATATCTTGGTGTATTTGATAATAAATATAATATCCAGCTAAGGAATGGATTGGTAACAGTATGTTTTCTTTGTATATCGTCAAACATAATTGCTGCAACTGAACAATATGCGTGTTTATTCTTTTTCCTCGTTTCTTTAGTCAATATTTCTTCATGAACTCTGTCTTCATATAAATAAATGTTACTTGGCTATATTTATCTCTTGCTTATTGTTTTATGGAAATAATTGGTAAACTGATCAAAGTCGTATTTGTTCTCTCTTGTCATTGTATCAGTTTAATTGAAGGAAAGACAATGCCATGAAATTGCTCTTTCGGTTCCTTTTAATTATGAAGATGCTTTTTCAGGGGTTTGACAGAGGGGGAAAGCTGCGAGCCATATGTGGTGGGGGTCGGTATGATCGTTTGCTTTCTACCTTTGGTGGTGACGATCTTCCTGCTTGTGGGTTCGGGTTTGGTGATGCTGTTATAGTAGAGGTACGTTTTAAGCTGAACTCTGCATCCTTACACAATCATAAAAAAATTCTATGAATTATATGCTTAATATTAATCATAGAACGTAACGTGGATGAACTTTTACTGAGGATATTATTAACGTCATGTTTTTTGATCAACTGTGTTCGACTGTTCATTATTTAATAACCACCTTGAATTGGCTCATAGTTAAGAATCTTTTGGTTCAAACCATGTAGGCCGTTTATCTCATGTATTATTAAGTATCCTACCAATTTCTTGATATCAAATCTTTTAGGATTAAGTTGTTGTTTGGTAGATTTAGTTGAGGAGTGTTAAACTAACTCATACTCAGGGAAAACTTTCAATTTTGTTTTATTGAGTATTGAACAAGGGGTGAAATCAATACCTTCTCAAAACTAAATTAGAACATTGTTCATCACTAGCCAAAAAAACCACACAATCTCAGCTAACTAAGCATTCAAATTCAAGTCATAGAAATGCTCAAATTGATCTATGAAAAATACAAAGTAGGTACAAAAAATTAAAAAAACAAAATCCTAAACTGAACAGTAAAATGCTTAATCCTAGGTAGAAGACATCCATATGATCAAAATAACCCACTTTTACCGAAAACTGTCATACCCCATCACAAGAACGCACATAGCTGACTGCAAATCATGTTGAAATAAAAAAACCAATGAAAACAATGGAACTGAGATGGTTCCCCACCCATTGGAAGTACACCCTTTAATTCAAGTCACTAATACCGATTTCATGGATCATTTCTATGATCAACATTATGTTGAACCAAACCACTCAAATCAACATGCCTTTTGAGGAATACACTTTTTTTTATCATACTTGTCGATAGCTCGGATTTGAGATATTTGTTTTGTGCCTGGACGTGCGATTTGGTTGTAGTTGCTCAAGGAAAAGAAGTTGCTGCCAGAACTGAATGTTGAAGTAGAGAACATAGTTTGTGCTCTGGATCCTGATCTCCAAGGCGCAGCTTCAAGAGTTGCTACCATTCTTAGGGGAAAAGGCCAAACTGTTGATTTGGTATTGGAGAATAAACCTCTCAAATGGTATCATTCACAAACCCGATCAACCTCATCAAAATTATCACAATTTCTGATCCACCCTGATTTGCTTCATCTTTTGCAGGGTGTTTAAACGAGCGACACGGATAAACGCTCAGCGGTTGATACTAGTCGGAAGTTCAGAATGGCAACGAGGCATGGTCGGAGTAAAAGATCTTTCCTCAGGTGAGCAACGCGAGGTCAAACTTGATGAACTCAACTAACTTAACCTTCATTCTTCTCTTCCACCGATTATTTATTCATATGTTTCGCTTAGATATCAAACTTTCCATTCAAACATTTTCCTATTTCAGATAGCTTTGTATTGAAATTGTTTGTGACTGATTTAACATGGGATACGTATTTAAATTAAAAGTTTAGTTCTCGAATTTTAATAATTGTGTCTAATGGGTTCTGTCTTTAAGATGTTAAGATGTTGGC

mRNA sequence

CTTCAGAAGGCGCCAATGAGCAATTGACTGTGCTGCGTCTCTGAACTGTCGACCACCATGGCTGCGAAGCCTTCATCGCTCCTTATCTTCCAACCTCGCCTCAATCTTCGTTTGAAGCCCTTCATTTTCTCTTCTTCGTCACTCGCTTTCAGTATCATTCTTAGAATCCCCTTTCCGAAGGCCTCATTCTTCGGTAACCGCAAATTTCATTCTTCTCTCAGCGCCGTCTCTTCCGCCCAGTCTACCGGTGACGGCGGAAATGGTGGCGGCAGGTCGGGGGCGCTATCTCCGACGCTGGTCACTGAAGAATTGCAGAAGATCGATGTTAACCCTCCCAAAGGAACCAGGGATTTTCCGCCGGAAGATATGCGACTCCGCAACTGGCTATTCAATAATTTCAGAGAGGTTTCACGAGTATATGGATTTGAGGAGGTTGATTTTCCGGTCCTTGAGTCGGAGGCGCTGTTCATTAGGAAAGCAGGTGAAGAAATAAGAGACCAGCTCTATTGTTTTGAAGATCGGGGTAATCGTCGAGTTGCTTTGAGACCTGAGCTTACTCCTTCTTTGGCAAGATTAGTGATCCAGAAAGGAAAATCTTTGTCCCTTCCACTGAAATGGTTTGCTATAGGGCAATGCTGGCGGTATGAGAGAATGACTAGAGGTCGGCGTCGTGAGCATTACCAATGGAATATGGATATCATTGGTGTACCCGAGGTTACGGCCGAAGCAGAGCTTCTATCTTCTATTGTTGCCTTTTTCAAACGAATCGGACTTACAGCAGCAGATGTTGGATTTAAGGTTTCCAGTCGAAAGGTTTTGCAAGAAGTATTAAAATGCTACGGAATACCTGAAAATATGTTTGGCAAGGTTTGCATCATCATAGACAAGATAGAAAAAATTCCATTGGATGAGATGAAGAAGGAACTGAAGGTTGTTGGTGTATCAGAGGATGCTATCGAGGAGATGTTGCAAGTTCTTTCAGTAAAGTCTTTGACAAAGTTAGAAGAGATACTTGGAGGTGGTGGTGAAGCCATGTCTGATCTGAAGCAGCTATTTTCTCTTGCTGAGAAGTTTGGTTATTCTGATTGGATCCAATTCGACGCGTCAATTGTCCGCGGCCTTGCTTATTACACTGGTATTGTTTTTGAGGGGTTTGACAGAGGGGGAAAGCTGCGAGCCATATGTGGTGGGGGTCGGTATGATCGTTTGCTTTCTACCTTTGGTGGTGACGATCTTCCTGCTTGTGGGTTCGGGTTTGGTGATGCTGTTATAGTAGAGTTGCTCAAGGAAAAGAAGTTGCTGCCAGAACTGAATGTTGAAGTAGAGAACATAGTTTGTGCTCTGGATCCTGATCTCCAAGGCGCAGCTTCAAGAGTTGCTACCATTCTTAGGGGAAAAGGCCAAACTGTTGATTTGGTATTGGAGAATAAACCTCTCAAATGGGTGTTTAAACGAGCGACACGGATAAACGCTCAGCGGTTGATACTAGTCGGAAGTTCAGAATGGCAACGAGGCATGGTCGGAGTAAAAGATCTTTCCTCAGGTGAGCAACGCGAGGTCAAACTTGATGAACTCAACTAACTTAACCTTCATTCTTCTCTTCCACCGATTATTTATTCATATGTTTCGCTTAGATATCAAACTTTCCATTCAAACATTTTCCTATTTCAGATAGCTTTGTATTGAAATTGTTTGTGACTGATTTAACATGGGATACGTATTTAAATTAAAAGTTTAGTTCTCGAATTTTAATAATTGTGTCTAATGGGTTCTGTCTTTAAGATGTTAAGATGTTGGC

Coding sequence (CDS)

ATGGCTGCGAAGCCTTCATCGCTCCTTATCTTCCAACCTCGCCTCAATCTTCGTTTGAAGCCCTTCATTTTCTCTTCTTCGTCACTCGCTTTCAGTATCATTCTTAGAATCCCCTTTCCGAAGGCCTCATTCTTCGGTAACCGCAAATTTCATTCTTCTCTCAGCGCCGTCTCTTCCGCCCAGTCTACCGGTGACGGCGGAAATGGTGGCGGCAGGTCGGGGGCGCTATCTCCGACGCTGGTCACTGAAGAATTGCAGAAGATCGATGTTAACCCTCCCAAAGGAACCAGGGATTTTCCGCCGGAAGATATGCGACTCCGCAACTGGCTATTCAATAATTTCAGAGAGGTTTCACGAGTATATGGATTTGAGGAGGTTGATTTTCCGGTCCTTGAGTCGGAGGCGCTGTTCATTAGGAAAGCAGGTGAAGAAATAAGAGACCAGCTCTATTGTTTTGAAGATCGGGGTAATCGTCGAGTTGCTTTGAGACCTGAGCTTACTCCTTCTTTGGCAAGATTAGTGATCCAGAAAGGAAAATCTTTGTCCCTTCCACTGAAATGGTTTGCTATAGGGCAATGCTGGCGGTATGAGAGAATGACTAGAGGTCGGCGTCGTGAGCATTACCAATGGAATATGGATATCATTGGTGTACCCGAGGTTACGGCCGAAGCAGAGCTTCTATCTTCTATTGTTGCCTTTTTCAAACGAATCGGACTTACAGCAGCAGATGTTGGATTTAAGGTTTCCAGTCGAAAGGTTTTGCAAGAAGTATTAAAATGCTACGGAATACCTGAAAATATGTTTGGCAAGGTTTGCATCATCATAGACAAGATAGAAAAAATTCCATTGGATGAGATGAAGAAGGAACTGAAGGTTGTTGGTGTATCAGAGGATGCTATCGAGGAGATGTTGCAAGTTCTTTCAGTAAAGTCTTTGACAAAGTTAGAAGAGATACTTGGAGGTGGTGGTGAAGCCATGTCTGATCTGAAGCAGCTATTTTCTCTTGCTGAGAAGTTTGGTTATTCTGATTGGATCCAATTCGACGCGTCAATTGTCCGCGGCCTTGCTTATTACACTGGTATTGTTTTTGAGGGGTTTGACAGAGGGGGAAAGCTGCGAGCCATATGTGGTGGGGGTCGGTATGATCGTTTGCTTTCTACCTTTGGTGGTGACGATCTTCCTGCTTGTGGGTTCGGGTTTGGTGATGCTGTTATAGTAGAGTTGCTCAAGGAAAAGAAGTTGCTGCCAGAACTGAATGTTGAAGTAGAGAACATAGTTTGTGCTCTGGATCCTGATCTCCAAGGCGCAGCTTCAAGAGTTGCTACCATTCTTAGGGGAAAAGGCCAAACTGTTGATTTGGTATTGGAGAATAAACCTCTCAAATGGGTGTTTAAACGAGCGACACGGATAAACGCTCAGCGGTTGATACTAGTCGGAAGTTCAGAATGGCAACGAGGCATGGTCGGAGTAAAAGATCTTTCCTCAGGTGAGCAACGCGAGGTCAAACTTGATGAACTCAACTAA

Protein sequence

MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVSSAQSTGDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRVYGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLTAADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAIEEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELNVEVENIVCALDPDLQGAASRVATILRGKGQTVDLVLENKPLKWVFKRATRINAQRLILVGSSEWQRGMVGVKDLSSGEQREVKLDELN

Homology

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BLAST of CmoCh03G012030 vs. ExPASy Swiss-Prot
Match: O82413 (Histidine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g46100 PE=2 SV=1)

HSP 1 Score: 753.4 bits (1944), Expect = 1.6e-216
Identity = 370/441 (83.90%), Postives = 410/441 (92.97%), Query Frame = 0


Query: 68  NGGGRSGALSPTLVTEE--LQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRVYGFEE 127
           NGGGRSG++   LVTEE   +KIDVNPPKGTRDFPPEDMRLRNWLFN+F+EVSR+YG+EE
Sbjct: 45  NGGGRSGSIVAPLVTEEDFHKKIDVNPPKGTRDFPPEDMRLRNWLFNHFKEVSRLYGYEE 104

Query: 128 VDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKSLSLPL 187
           VD+PVLE+EALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS+SLPL
Sbjct: 105 VDYPVLETEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKSVSLPL 164

Query: 188 KWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLTAADVG 247
           KWFAIGQCWRYERMTRGRRREHYQWNMDIIGVP+VTAEAEL+SSIV FFKRIG+TA+DVG
Sbjct: 165 KWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPQVTAEAELISSIVTFFKRIGITASDVG 224

Query: 248 FKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAIEEMLQ 307
           FKVSSRKVLQE+LK YG+PE++FG+VCIIIDKIEKIP+DE+KKEL   G+SEDAIE++LQ
Sbjct: 225 FKVSSRKVLQELLKKYGVPEDLFGRVCIIIDKIEKIPIDEIKKELGFTGISEDAIEQLLQ 284

Query: 308 VLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTGIVFEG 367
           VLSVKSL  LE+I+GG GEA++DLKQLFSLAEKFGYS+WIQFDAS+VRGLAYYTGIVFEG
Sbjct: 285 VLSVKSLDDLEDIIGGAGEAIADLKQLFSLAEKFGYSEWIQFDASVVRGLAYYTGIVFEG 344

Query: 368 FDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELNVEVENI 427
           FDR GKLRAICGGGRYDRLLST+GGDD PACGFGFGDAVIVELLKEK LLPEL  EVENI
Sbjct: 345 FDRKGKLRAICGGGRYDRLLSTYGGDDFPACGFGFGDAVIVELLKEKDLLPELGQEVENI 404

Query: 428 VCALDPDLQGAASRVATILRGKGQTVDLVLENKPLKWVFKRATRINAQRLILVGSSEWQR 487
           VCALD DLQGAA+ VAT LR KGQTVDLVLE+KPLKWVFKRA R+NA+RL+LVG +EW+ 
Sbjct: 405 VCALDKDLQGAAATVATALRDKGQTVDLVLESKPLKWVFKRAARVNARRLVLVGKTEWED 464

Query: 488 GMVGVKDLSSGEQREVKLDEL 507
           G V VK LSSGEQ +VKL +L
Sbjct: 465 GSVSVKVLSSGEQFQVKLSDL 485

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BLAST of CmoCh03G012030 vs. ExPASy Swiss-Prot
Match: Q8U431 (Histidine--tRNA ligase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) OX=186497 GN=hisS PE=3 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 1.1e-71
Identity = 154/423 (36.41%), Postives = 244/423 (57.68%), Query Frame = 0


Query: 94  KGTRDFPPEDMRLRNWLFNNFREVSRVYGFEEVDFPVLESEALFIRKAGEEIRDQLYCFE 153
           KGTRDF PE+M  R W+F   RE    YGF+EV  P +E   LF  ++GEE+  QLY F+
Sbjct: 6   KGTRDFLPEEMVKRRWVFERIRERFETYGFKEVLTPTMEYTKLFELRSGEEVVKQLYAFK 65

Query: 154 DRGNRRVALRPELTPSLARLVIQKGKSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNMD 213
           D+G R V+LRP++T S+ARL +   ++   P+KW+ I   +RYE    GR RE +Q  ++
Sbjct: 66  DKGGRDVSLRPDMTSSVARLYVNMFQTAPKPIKWYYIANMFRYEEPQSGRYREFWQAGVE 125

Query: 214 IIGVPEVTAEAELLSSIVAFFKRIGLTAADVGFKVSSRKVLQEVLKCYGIPENMFGKVCI 273
           +IG  +V A+AE+++  V  +   GL    V   +  R +L E  K  G+ +++   +  
Sbjct: 126 LIGSDKVEADAEVIALFVESYLATGLREFTV--NIGDRILLDEFAKMLGVKDDI--GLMR 185

Query: 274 IIDKIEKIPLDEMKKELKVVGVSEDAIEEMLQVLSVK----SLTKLEEILGGGGEAMSDL 333
           IIDK +K+P ++    LK  G+ ++ IE++L+++++K     +  L E L     A +++
Sbjct: 186 IIDKKDKLPQEDFINALKEFGLDKNGIEKVLELINIKGKPDEVLPLAEELFTSEVAKNEI 245

Query: 334 KQLFS---LAEKFGYSDWIQFDASIVRGLAYYTGIVFEGF---DRGGKLRAICGGGRYDR 393
            +L++   L E +   DWI+ D  I RG  YYT IVFE     D G  + +I GGGRYD 
Sbjct: 246 NRLYALIDLLEAYEVKDWIRIDLGIARGFDYYTSIVFEAIAPNDLG--IGSIGGGGRYDN 305

Query: 394 LLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELNVEVENIVCALDPDLQGAASRVATI 453
           L+  FGG   PA GF  G   ++ +L+ K L+PE+ +  +  V  +   ++  A R+AT 
Sbjct: 306 LIEVFGGKPTPATGFAIGIERLIPILEAKGLIPEVQIAPDVYVVPIGERMKKVAIRIATS 365

Query: 454 LRGKGQTVDLVLENKPLKWVFKRATRINAQRLILVGSSEWQRGMVGVKDLSSGEQREVKL 507
           LR  G   ++ L  + LK     A ++  ++ I+VG  + +RG+V V+D+ SGEQ EVK+
Sbjct: 366 LRKSGIKTEIELMGRKLKKAMDYANKVGVKKTIIVGEKDLERGVVTVRDMESGEQNEVKI 422

[Close]

BLAST of CmoCh03G012030 vs. ExPASy Swiss-Prot
Match: B1ZUW6 (Histidine--tRNA ligase OS=Opitutus terrae (strain DSM 11246 / JCM 15787 / PB90-1) OX=452637 GN=hisS PE=3 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 7.1e-71
Identity = 158/423 (37.35%), Postives = 237/423 (56.03%), Query Frame = 0


Query: 95  GTRDFPPEDMRLRNWLFNNFREVSRVYGFEEVDFPVLESEALFIRKAGEEIRDQLYCFED 154
           G R+F P+ +  RN +F  +R+ +  +GF E D PVLE   L+  K+G+EI  QL+ F D
Sbjct: 9   GFREFYPDALARRNHIFRLWRQTAVAFGFAEYDAPVLEPLELYKTKSGDEIEAQLFSFTD 68

Query: 155 RGNRRVALRPELTPSLARLVIQKGKSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDI 214
           +G R VALRPE+TP++ R+V  K  +L  P+KWF+I + +RYER  +GR R  +Q+N D+
Sbjct: 69  KGGREVALRPEMTPTVCRMVGAKANALKRPIKWFSIAEYYRYERAQKGRERAFFQFNADL 128

Query: 215 IGVPEVTAEAELLSSIVAFFKRIGLTAADVGFKVSSRKVLQEVLKCYGIPENMFGKVCII 274
              P   AE EL++ +    K  GLT  D   ++S R +    L+  G+ E     V   
Sbjct: 129 FAEPGPEAEIELIALLTQCLKAFGLTEQDFYIRLSDRNLWFFYLEALGLDEPRIRAVLSA 188

Query: 275 IDKIEKIPLDEMKKELKVVGVSEDAIEE-MLQVLSVKSLTKLEEILGG-GGEAMS----D 334
           +DK EK+  D  K   +  G  + A+++ +L+ L +K+L  LE+ L   GGE ++    D
Sbjct: 189 VDKFEKVGDDAFKPYAEQFGPLDLALKQRVLEFLQIKTLASLEQTLAPLGGEKLAARLGD 248

Query: 335 LKQLFSLAEKFGYSDWIQFDASIVRGLAYYTGIVFEGFDRGGKLRAICGGGRYDRLLSTF 394
            ++L       G S +I+ D  +VRGLAYYTG VFE FDR G+LRAI GGGRY+ L+   
Sbjct: 249 WRKLLDGLAAMGLSPFIEVDLGVVRGLAYYTGFVFEAFDRKGELRAIAGGGRYNDLVKKL 308

Query: 395 GGDDLPACGFGFGDAVIVELLKEKKLLPELNVEVENIVCALDPDLQGAASRVATI----- 454
           GG DLPA GF  GD  +  LL  + L+P   V+  ++ C +     GAA R A       
Sbjct: 309 GGPDLPAVGFAIGDVTLGLLLDARGLMPAF-VQASDVYCVIG----GAAERQAAFADVNA 368

Query: 455 LRGKGQTVDLVLENKPLKWVFKRATRINAQRLILVGSSEWQRGMVGVKDLSSGEQREVKL 507
           LR  G  VD  L++ P    FK A    A+  ++ G  E  + +V ++DL++  + +V  
Sbjct: 369 LRAAGFKVDYPLKDVPFGKQFKLAADSGAKLALIYGPDELAKNVVKLRDLTTRTETDVPR 426

[Close]

BLAST of CmoCh03G012030 vs. ExPASy Swiss-Prot
Match: Q9HLX5 (Histidine--tRNA ligase OS=Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165) OX=273075 GN=hisS PE=1 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 7.8e-70
Identity = 149/424 (35.14%), Postives = 236/424 (55.66%), Query Frame = 0


Query: 85  LQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRVYGFEEVDFPVLESEALFIRKAGEE 144
           + ++ +   +G RDF PEDM +  ++F    E +  +GF  +DFP LE   L+  K+GEE
Sbjct: 1   MYRLQIEKIRGFRDFYPEDMDVEKFIFKTAEEAAEAFGFRRIDFPSLEYLDLYRIKSGEE 60

Query: 145 IRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKSLSLPLKWFAIGQCWRYERMTRGRR 204
           +  Q Y F D+G R V L PE TPS  R+V  + K L  PL+W++  + WRYE    GR 
Sbjct: 61  LLQQTYSFVDKGGREVTLIPEATPSTVRMVTSR-KDLQRPLRWYSFPKVWRYEEPQAGRY 120

Query: 205 REHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLTAADVGFKVSSRKVLQEVLKCYGIP 264
           REHYQ+N DI G     A+AE+++   +   R+GL       +++SRK+++E++   G+ 
Sbjct: 121 REHYQFNADIFGSDSPEADAEVIALASSILDRLGLQDI-YEIRINSRKIMEEIIG--GMT 180

Query: 265 ENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAIEEMLQVLS-VKSLTKLEEILGGGG 324
            +    V  IID+  KI  +E   +L+  G+ ED +  +  + S  + + ++  I G   
Sbjct: 181 SSDPFSVFSIIDRYHKISREEFVDQLRSAGIGEDGVSMIADLCSGTRGIDEMARITGKSS 240

Query: 325 EAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTGIVFEGFDRGGKLRAICGGGRYDR 384
           E ++ +  +  L   +G  + +++D SIVRGL+YYTGIVFE +DR G+ RAI GGGRYD 
Sbjct: 241 EEIARMAAVEDLLASYGVKN-VRYDFSIVRGLSYYTGIVFEAYDRSGQFRAILGGGRYDN 300

Query: 385 LLSTFGGDDLPACGFGFGDAVIVELLKEKKL-LPELNVEVENIVCALDPDLQGAASRVAT 444
           L S   G+ +PA GFG GDAVI  LLK + + +P     V   +C +        +  + 
Sbjct: 301 LASLMSGESVPAVGFGMGDAVISLLLKRENVQIPREKKSV--YICRVGKINSSIMNEYSR 360

Query: 445 ILRGKGQTVDLVLENKPLKWVFKRATRINAQRLILVGSSEWQRGMVGVKDLSSGEQREVK 504
            LR +G  V + +  + L    K A+ I A   ++ G  + +RG+V ++++ +G Q  V 
Sbjct: 361 KLRERGMNVTVEIMERGLSAQLKYASAIGADFAVIFGERDLERGVVTIRNMYTGSQENVG 417

Query: 505 LDEL 507
           LD +
Sbjct: 421 LDSV 417

[Close]

BLAST of CmoCh03G012030 vs. ExPASy Swiss-Prot
Match: Q9UY31 (Histidine--tRNA ligase OS=Pyrococcus abyssi (strain GE5 / Orsay) OX=272844 GN=hisS PE=3 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 6.6e-69
Identity = 157/423 (37.12%), Postives = 236/423 (55.79%), Query Frame = 0


Query: 94  KGTRDFPPEDMRLRNWLFNNFREVSRVYGFEEVDFPVLESEALFIRKAGEEIRDQLYCFE 153
           KGTRDF PE+M  R W+F   REV   YGF+EV  PV+E   LF  ++GEE+  QLY F+
Sbjct: 6   KGTRDFLPEEMVKRRWVFEKIREVFETYGFKEVLTPVMEYTKLFQLRSGEEVVKQLYAFK 65

Query: 154 DRGNRRVALRPELTPSLARLVIQKGKSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNMD 213
           D+G R VALRP++T S+ARL +   ++   P+KW+ I   +RYE    GR RE +Q  ++
Sbjct: 66  DKGGRDVALRPDMTSSVARLYVNSFQTAPKPIKWYYIANMFRYEEPQSGRYREFWQAGVE 125

Query: 214 IIGVPEVTAEAELLSSIVAFFKRIGLTAADVGFKVSSRKVLQEVLKCYGIPENMFGKVCI 273
           +IG  ++ A+AE+++  V  +   GL   D    +  R +L E  K  G+ +++   +  
Sbjct: 126 LIGSDKIEADAEVIALFVDSYLSTGL--KDFTVNIGDRVLLDEFAKMLGVKDDI--GLMR 185

Query: 274 IIDKIEKIPLDEMKKELKVVGVSEDAIEEMLQVLSVK----SLTKLEEILGGGGEAMSDL 333
           IIDK +K+  +E  K L   G+ E+ IE++L ++ +K     +  L E L     A  ++
Sbjct: 186 IIDKKDKLSQEEFLKALGEFGLDENGIEKVLNLIEIKGKPDDVLPLAEELFTSERAKEEI 245

Query: 334 KQLFSLAEKFGY---SDWIQFDASIVRGLAYYTGIVFEGF---DRGGKLRAICGGGRYDR 393
            +L++L +   +    +WIQ D  I RG  YYT IVFE     D G  + +I GGGRYD 
Sbjct: 246 SRLYNLVDILSWYEVDEWIQIDLGIARGFDYYTSIVFEAIVPNDLG--IGSIGGGGRYDN 305

Query: 394 LLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELNVEVENIVCALDPDLQGAASRVATI 453
           L+  FGG   PA GF  G   ++ +L+ K LLPEL    +  V  +  D +  A+ + T 
Sbjct: 306 LIEVFGGKPTPATGFAIGIERLIPILEWKGLLPELKAGPDVFVIPVG-DSRDVATAIVTR 365

Query: 454 LRGKGQTVDLVLENKPLKWVFKRATRINAQRLILVGSSEWQRGMVGVKDLSSGEQREVKL 507
           LR  G   D+ L  + L+     A RI  +  I+VG  + +RG+V ++DL SG Q EV +
Sbjct: 366 LRKAGIRSDIELSGRKLRKALDYANRIGVRLSIIVGKRDLERGVVTIRDLESGNQVEVPV 421

[Close]

BLAST of CmoCh03G012030 vs. ExPASy TrEMBL
Match: A0A6J1EG18 (Histidine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111433048 PE=3 SV=1)

HSP 1 Score: 998.4 bits (2580), Expect = 1.1e-287
Identity = 507/507 (100.00%), Postives = 507/507 (100.00%), Query Frame = 0


Query: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVSSA 60
           MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVSSA
Sbjct: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVSSA 60

Query: 61  QSTGDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120
           QSTGDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV
Sbjct: 61  QSTGDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120

Query: 121 YGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180
           YGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS
Sbjct: 121 YGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT 240
           LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT
Sbjct: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT 240

Query: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300
           AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI
Sbjct: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300

Query: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360
           EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG
Sbjct: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360

Query: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELNV 420
           IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELNV
Sbjct: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELNV 420

Query: 421 EVENIVCALDPDLQGAASRVATILRGKGQTVDLVLENKPLKWVFKRATRINAQRLILVGS 480
           EVENIVCALDPDLQGAASRVATILRGKGQTVDLVLENKPLKWVFKRATRINAQRLILVGS
Sbjct: 421 EVENIVCALDPDLQGAASRVATILRGKGQTVDLVLENKPLKWVFKRATRINAQRLILVGS 480

Query: 481 SEWQRGMVGVKDLSSGEQREVKLDELN 508
           SEWQRGMVGVKDLSSGEQREVKLDELN
Sbjct: 481 SEWQRGMVGVKDLSSGEQREVKLDELN 507

[Close]

BLAST of CmoCh03G012030 vs. ExPASy TrEMBL
Match: A0A6J1ECF7 (Histidine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111433048 PE=3 SV=1)

HSP 1 Score: 992.6 bits (2565), Expect = 5.8e-286
Identity = 507/511 (99.22%), Postives = 507/511 (99.22%), Query Frame = 0


Query: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVSSA 60
           MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVSSA
Sbjct: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVSSA 60

Query: 61  QSTGDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120
           QSTGDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV
Sbjct: 61  QSTGDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120

Query: 121 YGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180
           YGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS
Sbjct: 121 YGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT 240
           LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT
Sbjct: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT 240

Query: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300
           AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI
Sbjct: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300

Query: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360
           EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG
Sbjct: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360

Query: 361 IVFE----GFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLP 420
           IVFE    GFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLP
Sbjct: 361 IVFEMLFQGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLP 420

Query: 421 ELNVEVENIVCALDPDLQGAASRVATILRGKGQTVDLVLENKPLKWVFKRATRINAQRLI 480
           ELNVEVENIVCALDPDLQGAASRVATILRGKGQTVDLVLENKPLKWVFKRATRINAQRLI
Sbjct: 421 ELNVEVENIVCALDPDLQGAASRVATILRGKGQTVDLVLENKPLKWVFKRATRINAQRLI 480

Query: 481 LVGSSEWQRGMVGVKDLSSGEQREVKLDELN 508
           LVGSSEWQRGMVGVKDLSSGEQREVKLDELN
Sbjct: 481 LVGSSEWQRGMVGVKDLSSGEQREVKLDELN 511

[Close]

BLAST of CmoCh03G012030 vs. ExPASy TrEMBL
Match: A0A6J1IL10 (Histidine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111478405 PE=3 SV=1)

HSP 1 Score: 986.5 bits (2549), Expect = 4.2e-284
Identity = 501/507 (98.82%), Postives = 504/507 (99.41%), Query Frame = 0


Query: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVSSA 60
           MAAKPSSLLIFQPRLNLRLKPFIFSS+SLAFSIILRIPFPK SFFGNRKFHSSLSAVSSA
Sbjct: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSTSLAFSIILRIPFPKPSFFGNRKFHSSLSAVSSA 60

Query: 61  QSTGDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120
           QS GDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV
Sbjct: 61  QSIGDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120

Query: 121 YGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180
           YGFEEVDFPVLESEALFIRKAGEEIR+QLYCFEDRGNRRVALRPELTPSLARLVIQKGKS
Sbjct: 121 YGFEEVDFPVLESEALFIRKAGEEIREQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT 240
           LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT
Sbjct: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT 240

Query: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300
           AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI
Sbjct: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300

Query: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360
           EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG
Sbjct: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360

Query: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELNV 420
           IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPEL+V
Sbjct: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELSV 420

Query: 421 EVENIVCALDPDLQGAASRVATILRGKGQTVDLVLENKPLKWVFKRATRINAQRLILVGS 480
           EVENIVCALDPDLQGAASRVATILR KGQTVDLVLENKPLKWVFKRATRINAQRLILVGS
Sbjct: 421 EVENIVCALDPDLQGAASRVATILREKGQTVDLVLENKPLKWVFKRATRINAQRLILVGS 480

Query: 481 SEWQRGMVGVKDLSSGEQREVKLDELN 508
           SEWQRGMVGVKDLSSGEQREVKLDELN
Sbjct: 481 SEWQRGMVGVKDLSSGEQREVKLDELN 507

[Close]

BLAST of CmoCh03G012030 vs. ExPASy TrEMBL
Match: A0A6J1IMK6 (Histidine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111478405 PE=3 SV=1)

HSP 1 Score: 980.7 bits (2534), Expect = 2.3e-282
Identity = 501/511 (98.04%), Postives = 504/511 (98.63%), Query Frame = 0


Query: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVSSA 60
           MAAKPSSLLIFQPRLNLRLKPFIFSS+SLAFSIILRIPFPK SFFGNRKFHSSLSAVSSA
Sbjct: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSTSLAFSIILRIPFPKPSFFGNRKFHSSLSAVSSA 60

Query: 61  QSTGDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120
           QS GDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV
Sbjct: 61  QSIGDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120

Query: 121 YGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180
           YGFEEVDFPVLESEALFIRKAGEEIR+QLYCFEDRGNRRVALRPELTPSLARLVIQKGKS
Sbjct: 121 YGFEEVDFPVLESEALFIRKAGEEIREQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT 240
           LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT
Sbjct: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT 240

Query: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300
           AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI
Sbjct: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300

Query: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360
           EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG
Sbjct: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360

Query: 361 IVFE----GFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLP 420
           IVFE    GFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLP
Sbjct: 361 IVFEMLFQGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLP 420

Query: 421 ELNVEVENIVCALDPDLQGAASRVATILRGKGQTVDLVLENKPLKWVFKRATRINAQRLI 480
           EL+VEVENIVCALDPDLQGAASRVATILR KGQTVDLVLENKPLKWVFKRATRINAQRLI
Sbjct: 421 ELSVEVENIVCALDPDLQGAASRVATILREKGQTVDLVLENKPLKWVFKRATRINAQRLI 480

Query: 481 LVGSSEWQRGMVGVKDLSSGEQREVKLDELN 508
           LVGSSEWQRGMVGVKDLSSGEQREVKLDELN
Sbjct: 481 LVGSSEWQRGMVGVKDLSSGEQREVKLDELN 511

[Close]

BLAST of CmoCh03G012030 vs. ExPASy TrEMBL
Match: A0A0A0KER6 (Histidine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_6G446550 PE=3 SV=1)

HSP 1 Score: 941.0 bits (2431), Expect = 2.0e-270
Identity = 471/507 (92.90%), Postives = 491/507 (96.84%), Query Frame = 0


Query: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVSSA 60
           MAAKPSSLLIFQP LNLRLKPFIFSSSSLAFSIILR PFPK SFF NR FHSSL+AVSSA
Sbjct: 1   MAAKPSSLLIFQPSLNLRLKPFIFSSSSLAFSIILRFPFPKPSFFLNRNFHSSLTAVSSA 60

Query: 61  QSTGDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120
           QS  DGG  GGRSGALSP   TEELQ+IDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV
Sbjct: 61  QSVSDGGTTGGRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120

Query: 121 YGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180
           YGFEEVD+PVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS
Sbjct: 121 YGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT 240
           LSLP+KWFAIGQCWRYERMTRGRRREHYQWNMDI+GVP VTAEAELLSSIV FFK++G+T
Sbjct: 181 LSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVPGVTAEAELLSSIVTFFKKVGIT 240

Query: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300
           A+DVGFKVSSRKVLQ+VLKCYGIP+NMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI
Sbjct: 241 ASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300

Query: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360
           EE+LQVLSVKSLTKLEE+LGGGGEA+SDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG
Sbjct: 301 EELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360

Query: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELNV 420
           IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDD+PACGFGFGDAVIVELLKEKKLLPELN 
Sbjct: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNA 420

Query: 421 EVENIVCALDPDLQGAASRVATILRGKGQTVDLVLENKPLKWVFKRATRINAQRLILVGS 480
           EVENIVCALDPDLQGAAS+VATILR KGQTVDLVLENKPLKWVFKRATRINA+RLILVGS
Sbjct: 421 EVENIVCALDPDLQGAASKVATILREKGQTVDLVLENKPLKWVFKRATRINARRLILVGS 480

Query: 481 SEWQRGMVGVKDLSSGEQREVKLDELN 508
           SEW+RGMVGVKDLSSGEQR+VKLDEL+
Sbjct: 481 SEWERGMVGVKDLSSGEQRDVKLDELD 507

[Close]

BLAST of CmoCh03G012030 vs. NCBI nr
Match: XP_022925718.1 (histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita moschata])

HSP 1 Score: 998.4 bits (2580), Expect = 2.2e-287
Identity = 507/507 (100.00%), Postives = 507/507 (100.00%), Query Frame = 0


Query: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVSSA 60
           MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVSSA
Sbjct: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVSSA 60

Query: 61  QSTGDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120
           QSTGDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV
Sbjct: 61  QSTGDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120

Query: 121 YGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180
           YGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS
Sbjct: 121 YGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT 240
           LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT
Sbjct: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT 240

Query: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300
           AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI
Sbjct: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300

Query: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360
           EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG
Sbjct: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360

Query: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELNV 420
           IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELNV
Sbjct: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELNV 420

Query: 421 EVENIVCALDPDLQGAASRVATILRGKGQTVDLVLENKPLKWVFKRATRINAQRLILVGS 480
           EVENIVCALDPDLQGAASRVATILRGKGQTVDLVLENKPLKWVFKRATRINAQRLILVGS
Sbjct: 421 EVENIVCALDPDLQGAASRVATILRGKGQTVDLVLENKPLKWVFKRATRINAQRLILVGS 480

Query: 481 SEWQRGMVGVKDLSSGEQREVKLDELN 508
           SEWQRGMVGVKDLSSGEQREVKLDELN
Sbjct: 481 SEWQRGMVGVKDLSSGEQREVKLDELN 507

[Close]

BLAST of CmoCh03G012030 vs. NCBI nr
Match: XP_023544233.1 (histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 993.4 bits (2567), Expect = 7.1e-286
Identity = 505/507 (99.61%), Postives = 505/507 (99.61%), Query Frame = 0


Query: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVSSA 60
           MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVSSA
Sbjct: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVSSA 60

Query: 61  QSTGDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120
           QSTGDGGNGGGRSGALSPTLV EELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV
Sbjct: 61  QSTGDGGNGGGRSGALSPTLVAEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120

Query: 121 YGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180
           YGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS
Sbjct: 121 YGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT 240
           LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT
Sbjct: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT 240

Query: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300
           AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI
Sbjct: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300

Query: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360
           EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG
Sbjct: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360

Query: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELNV 420
           IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELNV
Sbjct: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELNV 420

Query: 421 EVENIVCALDPDLQGAASRVATILRGKGQTVDLVLENKPLKWVFKRATRINAQRLILVGS 480
           EVENIVCALDPDLQGAASRVATILR KGQTVDLVLENKPLKWVFKRATRINAQRLILVGS
Sbjct: 421 EVENIVCALDPDLQGAASRVATILREKGQTVDLVLENKPLKWVFKRATRINAQRLILVGS 480

Query: 481 SEWQRGMVGVKDLSSGEQREVKLDELN 508
           SEWQRGMVGVKDLSSGEQREVKLDELN
Sbjct: 481 SEWQRGMVGVKDLSSGEQREVKLDELN 507

[Close]

BLAST of CmoCh03G012030 vs. NCBI nr
Match: XP_022925717.1 (histidine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Cucurbita moschata])

HSP 1 Score: 992.6 bits (2565), Expect = 1.2e-285
Identity = 507/511 (99.22%), Postives = 507/511 (99.22%), Query Frame = 0


Query: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVSSA 60
           MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVSSA
Sbjct: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVSSA 60

Query: 61  QSTGDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120
           QSTGDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV
Sbjct: 61  QSTGDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120

Query: 121 YGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180
           YGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS
Sbjct: 121 YGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT 240
           LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT
Sbjct: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT 240

Query: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300
           AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI
Sbjct: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300

Query: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360
           EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG
Sbjct: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360

Query: 361 IVFE----GFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLP 420
           IVFE    GFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLP
Sbjct: 361 IVFEMLFQGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLP 420

Query: 421 ELNVEVENIVCALDPDLQGAASRVATILRGKGQTVDLVLENKPLKWVFKRATRINAQRLI 480
           ELNVEVENIVCALDPDLQGAASRVATILRGKGQTVDLVLENKPLKWVFKRATRINAQRLI
Sbjct: 421 ELNVEVENIVCALDPDLQGAASRVATILRGKGQTVDLVLENKPLKWVFKRATRINAQRLI 480

Query: 481 LVGSSEWQRGMVGVKDLSSGEQREVKLDELN 508
           LVGSSEWQRGMVGVKDLSSGEQREVKLDELN
Sbjct: 481 LVGSSEWQRGMVGVKDLSSGEQREVKLDELN 511

[Close]

BLAST of CmoCh03G012030 vs. NCBI nr
Match: KAG6604395.1 (Histidine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 991.9 bits (2563), Expect = 2.1e-285
Identity = 504/507 (99.41%), Postives = 505/507 (99.61%), Query Frame = 0


Query: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVSSA 60
           MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVSSA
Sbjct: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVSSA 60

Query: 61  QSTGDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120
           QSTGDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV
Sbjct: 61  QSTGDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120

Query: 121 YGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180
           YGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS
Sbjct: 121 YGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT 240
           LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT
Sbjct: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT 240

Query: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300
           AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI
Sbjct: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300

Query: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360
           EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG
Sbjct: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360

Query: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELNV 420
           IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELNV
Sbjct: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELNV 420

Query: 421 EVENIVCALDPDLQGAASRVATILRGKGQTVDLVLENKPLKWVFKRATRINAQRLILVGS 480
           EVENIVCALDPDLQGAASRVATILR KGQTVDLVLENKPLKWVFKRA RINAQRLILVGS
Sbjct: 421 EVENIVCALDPDLQGAASRVATILREKGQTVDLVLENKPLKWVFKRAARINAQRLILVGS 480

Query: 481 SEWQRGMVGVKDLSSGEQREVKLDELN 508
           SEWQRGMVGVKDLSSGEQREVKL+ELN
Sbjct: 481 SEWQRGMVGVKDLSSGEQREVKLEELN 507

[Close]

BLAST of CmoCh03G012030 vs. NCBI nr
Match: XP_023544232.1 (histidine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 987.6 bits (2552), Expect = 3.9e-284
Identity = 505/511 (98.83%), Postives = 505/511 (98.83%), Query Frame = 0


Query: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVSSA 60
           MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVSSA
Sbjct: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVSSA 60

Query: 61  QSTGDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120
           QSTGDGGNGGGRSGALSPTLV EELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV
Sbjct: 61  QSTGDGGNGGGRSGALSPTLVAEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120

Query: 121 YGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180
           YGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS
Sbjct: 121 YGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT 240
           LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT
Sbjct: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT 240

Query: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300
           AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI
Sbjct: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300

Query: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360
           EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG
Sbjct: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360

Query: 361 IVFE----GFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLP 420
           IVFE    GFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLP
Sbjct: 361 IVFEMLFQGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLP 420

Query: 421 ELNVEVENIVCALDPDLQGAASRVATILRGKGQTVDLVLENKPLKWVFKRATRINAQRLI 480
           ELNVEVENIVCALDPDLQGAASRVATILR KGQTVDLVLENKPLKWVFKRATRINAQRLI
Sbjct: 421 ELNVEVENIVCALDPDLQGAASRVATILREKGQTVDLVLENKPLKWVFKRATRINAQRLI 480

Query: 481 LVGSSEWQRGMVGVKDLSSGEQREVKLDELN 508
           LVGSSEWQRGMVGVKDLSSGEQREVKLDELN
Sbjct: 481 LVGSSEWQRGMVGVKDLSSGEQREVKLDELN 511

[Close]

BLAST of CmoCh03G012030 vs. TAIR 10
Match: AT3G46100.1 (Histidyl-tRNA synthetase 1 )

HSP 1 Score: 753.4 bits (1944), Expect = 1.1e-217
Identity = 370/441 (83.90%), Postives = 410/441 (92.97%), Query Frame = 0


Query: 68  NGGGRSGALSPTLVTEE--LQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRVYGFEE 127
           NGGGRSG++   LVTEE   +KIDVNPPKGTRDFPPEDMRLRNWLFN+F+EVSR+YG+EE
Sbjct: 45  NGGGRSGSIVAPLVTEEDFHKKIDVNPPKGTRDFPPEDMRLRNWLFNHFKEVSRLYGYEE 104

Query: 128 VDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKSLSLPL 187
           VD+PVLE+EALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS+SLPL
Sbjct: 105 VDYPVLETEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKSVSLPL 164

Query: 188 KWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLTAADVG 247
           KWFAIGQCWRYERMTRGRRREHYQWNMDIIGVP+VTAEAEL+SSIV FFKRIG+TA+DVG
Sbjct: 165 KWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPQVTAEAELISSIVTFFKRIGITASDVG 224

Query: 248 FKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAIEEMLQ 307
           FKVSSRKVLQE+LK YG+PE++FG+VCIIIDKIEKIP+DE+KKEL   G+SEDAIE++LQ
Sbjct: 225 FKVSSRKVLQELLKKYGVPEDLFGRVCIIIDKIEKIPIDEIKKELGFTGISEDAIEQLLQ 284

Query: 308 VLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTGIVFEG 367
           VLSVKSL  LE+I+GG GEA++DLKQLFSLAEKFGYS+WIQFDAS+VRGLAYYTGIVFEG
Sbjct: 285 VLSVKSLDDLEDIIGGAGEAIADLKQLFSLAEKFGYSEWIQFDASVVRGLAYYTGIVFEG 344

Query: 368 FDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELNVEVENI 427
           FDR GKLRAICGGGRYDRLLST+GGDD PACGFGFGDAVIVELLKEK LLPEL  EVENI
Sbjct: 345 FDRKGKLRAICGGGRYDRLLSTYGGDDFPACGFGFGDAVIVELLKEKDLLPELGQEVENI 404

Query: 428 VCALDPDLQGAASRVATILRGKGQTVDLVLENKPLKWVFKRATRINAQRLILVGSSEWQR 487
           VCALD DLQGAA+ VAT LR KGQTVDLVLE+KPLKWVFKRA R+NA+RL+LVG +EW+ 
Sbjct: 405 VCALDKDLQGAAATVATALRDKGQTVDLVLESKPLKWVFKRAARVNARRLVLVGKTEWED 464

Query: 488 GMVGVKDLSSGEQREVKLDEL 507
           G V VK LSSGEQ +VKL +L
Sbjct: 465 GSVSVKVLSSGEQFQVKLSDL 485

[Close]

BLAST of CmoCh03G012030 vs. TAIR 10
Match: AT5G03406.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 387.9 bits (995), Expect = 1.3e-107
Identity = 182/218 (83.49%), Postives = 206/218 (94.50%), Query Frame = 0


Query: 68  NGGGRSGAL-SPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRVYGFEEV 127
           NGGGRSG++ +P +V EE Q IDVNPPKGTRDFPPEDMRLRNWLFN+F+EVSR++G+EEV
Sbjct: 46  NGGGRSGSIVAPLVVNEEFQNIDVNPPKGTRDFPPEDMRLRNWLFNHFKEVSRLFGYEEV 105

Query: 128 DFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKSLSLPLK 187
           DFPVLE+EALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS+SLPLK
Sbjct: 106 DFPVLETEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKSVSLPLK 165

Query: 188 WFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLTAADVGF 247
           WFA+GQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAEL+SSI+ FFKRIG+TA+DVGF
Sbjct: 166 WFAVGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELISSIMTFFKRIGITASDVGF 225

Query: 248 KVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLD 285
           KVSSRKVLQE LK YG+PE++FG+VC IIDK+  + ++
Sbjct: 226 KVSSRKVLQEFLKKYGVPEDLFGRVCFIIDKVSNLNIN 263

[Close]

BLAST of CmoCh03G012030 vs. TAIR 10
Match: AT3G02760.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 164.1 bits (414), Expect = 3.0e-40
Identity = 130/425 (30.59%), Postives = 206/425 (48.47%), Query Frame = 0


Query: 93  PKGTRDFPPEDMRLRNWLFNNFREVSRVYGFEEVDFPVLESEALFIRKAGEEIRDQLYCF 152
           PKGTRDF  E M +R   F+  + V + +G   +D PV E     + K GE+ +  +Y  
Sbjct: 449 PKGTRDFAKEQMAVREKAFSIIQNVFKRHGATALDTPVFELRETLMGKYGEDSK-LVYDI 508

Query: 153 EDRGNRRVALRPELTPSLARLVIQKGKSLSLPLKWFAIGQCWRYERMTRGRRREHYQWNM 212
            D+G    +LR +LT   AR V   G +     K + I + +R +  ++GR RE YQ + 
Sbjct: 509 ADQGGELCSLRYDLTVPFARYVAMNGIT---SFKRYQIAKVYRRDNPSKGRYREFYQCDF 568

Query: 213 DIIGVPE-VTAEAELLSSIVAFFKRIGLTAADVGFKVSSRKVLQEVLKCYGIPENMFGKV 272
           DI G+ E +  + E++  +        L   D   K++ RK+L  +L+  G+P   F  +
Sbjct: 569 DIAGLFEPMGPDFEIVKILTELLDE--LEIGDYEVKLNHRKLLDGMLEICGVPPEKFRTI 628

Query: 273 CIIIDKIEKIPLDEMKKEL-KVVGVSEDAIEEMLQVLSVKS-----LTKL----EEILG- 332
           C  IDK++K   +++KKE+ +  G+S +  + +   +  K      L+KL     E L  
Sbjct: 629 CSSIDKLDKQSFEQVKKEMVEEKGLSSEIADRIGNFVKEKGAPLELLSKLRQEGSEFLDN 688

Query: 333 -GGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTGIVFEGFDRGGKLRAICGGG 392
               EA+ +L  +F   ++   S+ I FD S+ RGL YYTG++FE    G ++ +I  GG
Sbjct: 689 QSSREALDELSIMFEALKRSKCSERIVFDLSLARGLDYYTGVIFEAVCIGAEVGSIGAGG 748

Query: 393 RYDRLLSTFGGDDLPACGFGFG-DAV--IVELLKEKKLLPELNVEVENIVCAL-DPDLQG 452
           RYD L+  FG   +PA G   G + V  I+E L EK+       E + +V  + D  L  
Sbjct: 749 RYDNLIGMFGTKQVPAVGMSLGIERVFNIMEELNEKQKQVIRPTETQVLVSIMVDNKLAE 808

Query: 453 AASRVATILRGKGQTVDLVLENKPLKWVFKRATRINAQRLILVGSSEWQRGMVGVKDLSS 501
           AA  V+ +   K     LV + K     F RA       +++VG  E     V +K L  
Sbjct: 809 AAELVSQLWGAKINAEYLVSKRKEKH--FNRAKESGIPWMVMVGEKELSGSFVTLKKLEK 865

The following BLAST results are available for this feature:

BLAST of CmoCh03G012030 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O82413	1.6e-216	83.90	Histidine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3...	[more]
Q8U431	1.1e-71	36.41	Histidine--tRNA ligase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JC...	[more]
B1ZUW6	7.1e-71	37.35	Histidine--tRNA ligase OS=Opitutus terrae (strain DSM 11246 / JCM 15787 / PB90-1...	[more]
Q9HLX5	7.8e-70	35.14	Histidine--tRNA ligase OS=Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728...	[more]
Q9UY31	6.6e-69	37.12	Histidine--tRNA ligase OS=Pyrococcus abyssi (strain GE5 / Orsay) OX=272844 GN=hi...	[more]

BLAST of CmoCh03G012030 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EG18	1.1e-287	100.00	Histidine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111433048 PE=3 SV=1	[more]
A0A6J1ECF7	5.8e-286	99.22	Histidine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111433048 PE=3 SV=1	[more]
A0A6J1IL10	4.2e-284	98.82	Histidine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111478405 PE=3 SV=1	[more]
A0A6J1IMK6	2.3e-282	98.04	Histidine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111478405 PE=3 SV=1	[more]
A0A0A0KER6	2.0e-270	92.90	Histidine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_6G446550 PE=3 SV=1	[more]

BLAST of CmoCh03G012030 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022925718.1	2.2e-287	100.00	histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita moscha...	[more]
XP_023544233.1	7.1e-286	99.61	histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita pepo s...	[more]
XP_022925717.1	1.2e-285	99.22	histidine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Cucurbita moscha...	[more]
KAG6604395.1	2.1e-285	99.41	Histidine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosp...	[more]
XP_023544232.1	3.9e-284	98.83	histidine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Cucurbita pepo s...	[more]

BLAST of CmoCh03G012030 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G46100.1	1.1e-217	83.90	Histidyl-tRNA synthetase 1	[more]
AT5G03406.1	1.3e-107	83.49	Class II aaRS and biotin synthetases superfamily protein	[more]
AT3G02760.1	3.0e-40	30.59	Class II aaRS and biotin synthetases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004516	Histidine-tRNA ligase/ATP phosphoribosyltransferase regulatory subunit	PIRSF	PIRSF001549	His-tRNA_synth	coord: 86..507 e-value: 1.6E-146 score: 486.4
IPR004516	Histidine-tRNA ligase/ATP phosphoribosyltransferase regulatory subunit	PANTHER	PTHR43707	HISTIDYL-TRNA SYNTHETASE	coord: 86..506
IPR036621	Anticodon-binding domain superfamily	GENE3D	3.40.50.800		coord: 415..507 e-value: 2.2E-18 score: 67.7
IPR015807	Histidine-tRNA ligase	TIGRFAM	TIGR00442	TIGR00442	coord: 92..502 e-value: 6.7E-104 score: 346.0
IPR015807	Histidine-tRNA ligase	HAMAP	MF_00127	His_tRNA_synth	coord: 88..507 score: 25.174149
IPR041715	Class II Histidinyl-tRNA synthetase (HisRS)-like catalytic core domain	PFAM	PF13393	tRNA-synt_His	coord: 95..399 e-value: 1.7E-46 score: 158.9
IPR041715	Class II Histidinyl-tRNA synthetase (HisRS)-like catalytic core domain	CDD	cd00773	HisRS-like_core	coord: 103..410 e-value: 4.84463E-98 score: 294.895
IPR004154	Anticodon-binding	PFAM	PF03129	HGTP_anticodon	coord: 428..506 e-value: 8.1E-9 score: 35.5
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 86..414 e-value: 8.8E-115 score: 385.1
None	No IPR available	PANTHER	PTHR43707:SF1	HISTIDINE--TRNA LIGASE, MITOCHONDRIAL-RELATED	coord: 86..506
None	No IPR available	SUPERFAMILY	55681	Class II aaRS and biotin synthetases	coord: 92..413
None	No IPR available	SUPERFAMILY	52954	Class II aaRS ABD-related	coord: 422..506
IPR006195	Aminoacyl-tRNA synthetase, class II	PROSITE	PS50862	AA_TRNA_LIGASE_II	coord: 95..416 score: 13.020735

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh03G012030.1	CmoCh03G012030.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006427	histidyl-tRNA aminoacylation
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004821	histidine-tRNA ligase activity