CmoCh03G011860 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh03G011860
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Threonine dehydratase
Location	Cmo_Chr03: 9263027 .. 9265412 (-)
RNA-Seq Expression	CmoCh03G011860
Synteny	CmoCh03G011860

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCATCATCCTTTCCTCTCAATGTAGCAGCCGACGGTTCGGTTGCAGCGGTTCCGGAGAAGAATAAGGGACATTCGTGGAAGGACCTTGAGGTCGAGGAAGGGGTAATCGTGGAAAGGAAATTCCCTCCCGCCAAACCGGATACTAAGAGGCAGATGGAGTATCTTCAAAAAATATTAGGTTCAAATGTGTACGATGTGGCTATTGAAACCCCATTGCAGCTTGCTCCCCTCTTGTCTACTCGAATGGGGGTCAATCTGTGGGTCAAGAGGGAGGATGCACAGCAGGTAACCACTATCCATCACTCAAAATAATTTAGGCAGGGAGAAGTTTCAACACCCTTCTAACTCACAATTCGTAATGTAGGTATTCTCATTCAAGATTCGAGGAGCGTATAATATGATTTCCCTACTCCCCAAAGAAAAATTGAAAAAGGGGGTTATCTGTGCGTCCGCTGGGAATCATGCCCAGGGAGTTGCATTAGCTGGTCAGCGATTGAAAACTGAAGCCCACATTGTTATGCCAAGAACTACTCCTCAAATTAAGGTCGCCACTACTAAACTCATATCATTTTTTTTTCTTTGAATTTTTGAGGTTTGAAATTAACGGTGAATCATAAAAATATATAGATAGATGCAGTTGAGGACTTGGGCGGAATTGTCGAACTGTTTGGCAATGATTTTAATGAAGCGCAAAAAAGAGCTCAAGAGCGGAGCAAAGAGGAAGACCTCACAATCATACCTCCTTTTGATGACGAAGATGTTATAGCAGGCCAAGGTACAATTGGGATGGAAATTGGGCGTCAAATACGGGGCAAAATCCATGCTATTTTTGTTCCTGTTGGGGGTGGTGGCCTCGCTGCTGGTATTGTCTCTTTTTACAAGCTGGTTTATCCTGATGTAAGTGCCTCCATATGTGTTTCTTGTTCTTGTTCTTGTTGTTGATTAAATTTTGGGTTGGTTGATTAGTGTTGTTGTGTTGATGATACAGGTAAAGGTATATGGGGTGGAACCAAATGATCAGAACTCCATGGCGCTGGCATTGCATCTTGGGAAGGTAGTGTTTGTGGGCGATATTGGAAATTTTGCAGATGGAGTAGCTGTTAGACAAGTGGGGAACGAGACTTTTCGAATCTGCAATGAACTCCTGGATGGTGTAATTCTTGTTAAAAAAGAATCAATAGCTTCCGCCTTGAAGGTTTGGATGGATTAATTCTGTGTTACTTCCAATAATGATTCATAATATTGTTCTAAGAGAGATACCTGGTTGTATGCATGTGACAGGACATGTTTAACGACGGCAGGAACATCTTAGAACCTTCCGGTGCTCTTTCCATTGCTGGAGCCGTGGCCTACTGCAGGCGTCATGGCATTAGGAGAGAAAATGTTGTGGCTGTTACTAGTGGTGCAAATATGAACTTTGACCAATTGGGTCATATTGCCGATCTTGCCAATTCTGATCAGTCTATTTTGGCAAGTTGGTTGTCAGAGTGCCCAAACAGCTTAGAACCACTTACCCGCGTGGTCGACCGAGTGAGTTCATTCATTCATTTCTTTAGATATAGTTTTGTGGTTTCAAAGTGATTTTGACGTGACAAAATGTGGTTGTTATTTCATAATTTGGGCCTTGAGTAACATCTTTAATGGGGTGACTTTGGCTCTACAGATGCATTTCAACATTACAGAAATGACTTATAGATTTAGCTCTGGCTCAGTAGACGCTCTGCTTGTTTATAAGTAAGTTCTCAAATTTATGTCAACGAAACATACTTTCTCATTATAGATTTAACTTGGTTTATATAATGATATGCAGAGTTAGACCAATGAAGAAATACCTTGAAATTGAAACTTTGGTGGCAGAGTTGATTTCTTGTGGGTTTAAAACTTACACTTTATCAGATAATGAAGTTGTTAGAAACCATCTCCGTTTCATGGTAAATACGTATACAGATTCCTTGTTCAAATTAAATTTGGAAAACGCCTCAAATATTAATTTGTAATCAACACTTTTATTTTTCATTTCCAGGTGGGAGGCAGACAAAACGTCGAAAACGAAACTCTCTTCCGTTTTACTTTCCCAGAAAAGGCAGGTGCTCTCAACCACTTCCTCAAAGGCTTCCAGCCCAACTGGAACATTTCTCTGTTGCACCATCGTGACCAGGTACATGTTCGAGTCTCGAGTTTCTTATTAGACCCTGTTTTGATAATCGACCATCAATTGGGTAATGTTTTTGTTGAACAGGGTATTATTACTTCACAAGTGTTAATTGGAATCCAACTTGAGAAATCAGAGGAGAAAAAGTTTGATGAGTATGCGAAGGAAGTTGGGTATCAATATGAGGCAATATCTCCAGATGATTCTGCCCATGTTCTTGCAAAACTCTAG

mRNA sequence

ATGGCATCATCCTTTCCTCTCAATGTAGCAGCCGACGGTTCGGTTGCAGCGGTTCCGGAGAAGAATAAGGGACATTCGTGGAAGGACCTTGAGGTCGAGGAAGGGGTAATCGTGGAAAGGAAATTCCCTCCCGCCAAACCGGATACTAAGAGGCAGATGGAGTATCTTCAAAAAATATTAGGTTCAAATGTGTACGATGTGGCTATTGAAACCCCATTGCAGCTTGCTCCCCTCTTGTCTACTCGAATGGGGGTCAATCTGTGGGTCAAGAGGGAGGATGCACAGCAGGTATTCTCATTCAAGATTCGAGGAGCGTATAATATGATTTCCCTACTCCCCAAAGAAAAATTGAAAAAGGGGGTTATCTGTGCGTCCGCTGGGAATCATGCCCAGGGAGTTGCATTAGCTGGTCAGCGATTGAAAACTGAAGCCCACATTGTTATGCCAAGAACTACTCCTCAAATTAAGATAGATGCAGTTGAGGACTTGGGCGGAATTGTCGAACTGTTTGGCAATGATTTTAATGAAGCGCAAAAAAGAGCTCAAGAGCGGAGCAAAGAGGAAGACCTCACAATCATACCTCCTTTTGATGACGAAGATGTTATAGCAGGCCAAGGTACAATTGGGATGGAAATTGGGCGTCAAATACGGGGCAAAATCCATGCTATTTTTGTTCCTGTTGGGGGTGGTGGCCTCGCTGCTGGTATTGTCTCTTTTTACAAGCTGGTTTATCCTGATGTAAAGGTATATGGGGTGGAACCAAATGATCAGAACTCCATGGCGCTGGCATTGCATCTTGGGAAGGTAGTGTTTGTGGGCGATATTGGAAATTTTGCAGATGGAGTAGCTGTTAGACAAGTGGGGAACGAGACTTTTCGAATCTGCAATGAACTCCTGGATGGTGTAATTCTTGTTAAAAAAGAATCAATAGCTTCCGCCTTGAAGGACATGTTTAACGACGGCAGGAACATCTTAGAACCTTCCGGTGCTCTTTCCATTGCTGGAGCCGTGGCCTACTGCAGGCGTCATGGCATTAGGAGAGAAAATGTTGTGGCTGTTACTAGTGGTGCAAATATGAACTTTGACCAATTGGGTCATATTGCCGATCTTGCCAATTCTGATCAGTCTATTTTGGCAAGTTGGTTGTCAGAGTGCCCAAACAGCTTAGAACCACTTACCCGCGTGGTCGACCGAATGCATTTCAACATTACAGAAATGACTTATAGATTTAGCTCTGGCTCAGTAGACGCTCTGCTTGTTTATAAAGTTAGACCAATGAAGAAATACCTTGAAATTGAAACTTTGGTGGCAGAGTTGATTTCTTGTGGGTTTAAAACTTACACTTTATCAGATAATGAAGTTGTTAGAAACCATCTCCGTTTCATGGTGGGAGGCAGACAAAACGTCGAAAACGAAACTCTCTTCCGTTTTACTTTCCCAGAAAAGGCAGGTGCTCTCAACCACTTCCTCAAAGGCTTCCAGCCCAACTGGAACATTTCTCTGTTGCACCATCGTGACCAGGGTATTATTACTTCACAAGTGTTAATTGGAATCCAACTTGAGAAATCAGAGGAGAAAAAGTTTGATGAGTATGCGAAGGAAGTTGGGTATCAATATGAGGCAATATCTCCAGATGATTCTGCCCATGTTCTTGCAAAACTCTAG

Coding sequence (CDS)

ATGGCATCATCCTTTCCTCTCAATGTAGCAGCCGACGGTTCGGTTGCAGCGGTTCCGGAGAAGAATAAGGGACATTCGTGGAAGGACCTTGAGGTCGAGGAAGGGGTAATCGTGGAAAGGAAATTCCCTCCCGCCAAACCGGATACTAAGAGGCAGATGGAGTATCTTCAAAAAATATTAGGTTCAAATGTGTACGATGTGGCTATTGAAACCCCATTGCAGCTTGCTCCCCTCTTGTCTACTCGAATGGGGGTCAATCTGTGGGTCAAGAGGGAGGATGCACAGCAGGTATTCTCATTCAAGATTCGAGGAGCGTATAATATGATTTCCCTACTCCCCAAAGAAAAATTGAAAAAGGGGGTTATCTGTGCGTCCGCTGGGAATCATGCCCAGGGAGTTGCATTAGCTGGTCAGCGATTGAAAACTGAAGCCCACATTGTTATGCCAAGAACTACTCCTCAAATTAAGATAGATGCAGTTGAGGACTTGGGCGGAATTGTCGAACTGTTTGGCAATGATTTTAATGAAGCGCAAAAAAGAGCTCAAGAGCGGAGCAAAGAGGAAGACCTCACAATCATACCTCCTTTTGATGACGAAGATGTTATAGCAGGCCAAGGTACAATTGGGATGGAAATTGGGCGTCAAATACGGGGCAAAATCCATGCTATTTTTGTTCCTGTTGGGGGTGGTGGCCTCGCTGCTGGTATTGTCTCTTTTTACAAGCTGGTTTATCCTGATGTAAAGGTATATGGGGTGGAACCAAATGATCAGAACTCCATGGCGCTGGCATTGCATCTTGGGAAGGTAGTGTTTGTGGGCGATATTGGAAATTTTGCAGATGGAGTAGCTGTTAGACAAGTGGGGAACGAGACTTTTCGAATCTGCAATGAACTCCTGGATGGTGTAATTCTTGTTAAAAAAGAATCAATAGCTTCCGCCTTGAAGGACATGTTTAACGACGGCAGGAACATCTTAGAACCTTCCGGTGCTCTTTCCATTGCTGGAGCCGTGGCCTACTGCAGGCGTCATGGCATTAGGAGAGAAAATGTTGTGGCTGTTACTAGTGGTGCAAATATGAACTTTGACCAATTGGGTCATATTGCCGATCTTGCCAATTCTGATCAGTCTATTTTGGCAAGTTGGTTGTCAGAGTGCCCAAACAGCTTAGAACCACTTACCCGCGTGGTCGACCGAATGCATTTCAACATTACAGAAATGACTTATAGATTTAGCTCTGGCTCAGTAGACGCTCTGCTTGTTTATAAAGTTAGACCAATGAAGAAATACCTTGAAATTGAAACTTTGGTGGCAGAGTTGATTTCTTGTGGGTTTAAAACTTACACTTTATCAGATAATGAAGTTGTTAGAAACCATCTCCGTTTCATGGTGGGAGGCAGACAAAACGTCGAAAACGAAACTCTCTTCCGTTTTACTTTCCCAGAAAAGGCAGGTGCTCTCAACCACTTCCTCAAAGGCTTCCAGCCCAACTGGAACATTTCTCTGTTGCACCATCGTGACCAGGGTATTATTACTTCACAAGTGTTAATTGGAATCCAACTTGAGAAATCAGAGGAGAAAAAGTTTGATGAGTATGCGAAGGAAGTTGGGTATCAATATGAGGCAATATCTCCAGATGATTCTGCCCATGTTCTTGCAAAACTCTAG

Protein sequence

MASSFPLNVAADGSVAAVPEKNKGHSWKDLEVEEGVIVERKFPPAKPDTKRQMEYLQKILGSNVYDVAIETPLQLAPLLSTRMGVNLWVKREDAQQVFSFKIRGAYNMISLLPKEKLKKGVICASAGNHAQGVALAGQRLKTEAHIVMPRTTPQIKIDAVEDLGGIVELFGNDFNEAQKRAQERSKEEDLTIIPPFDDEDVIAGQGTIGMEIGRQIRGKIHAIFVPVGGGGLAAGIVSFYKLVYPDVKVYGVEPNDQNSMALALHLGKVVFVGDIGNFADGVAVRQVGNETFRICNELLDGVILVKKESIASALKDMFNDGRNILEPSGALSIAGAVAYCRRHGIRRENVVAVTSGANMNFDQLGHIADLANSDQSILASWLSECPNSLEPLTRVVDRMHFNITEMTYRFSSGSVDALLVYKVRPMKKYLEIETLVAELISCGFKTYTLSDNEVVRNHLRFMVGGRQNVENETLFRFTFPEKAGALNHFLKGFQPNWNISLLHHRDQGIITSQVLIGIQLEKSEEKKFDEYAKEVGYQYEAISPDDSAHVLAKL

Homology

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BLAST of CmoCh03G011860 vs. ExPASy Swiss-Prot
Match: A0FKE6 (Threonine dehydratase 1 biosynthetic, chloroplastic OS=Solanum lycopersicum OX=4081 GN=TD1 PE=1 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 3.1e-152
Identity = 272/501 (54.29%), Postives = 365/501 (72.85%), Query Frame = 0


Query: 54  EYLQKILGSNVYDVAIETPLQLAPLLSTRMGVNLWVKREDAQQVFSFKIRGAYNMISLLP 113
           EYL  IL S VYDVA ETPLQ AP LS R+GVN+W+KRED Q VFSFKIRGAYNM++ LP
Sbjct: 107 EYLTNILSSKVYDVAYETPLQKAPKLSERLGVNVWLKREDLQPVFSFKIRGAYNMMAKLP 166

Query: 114 KEKLKKGVICASAGNHAQGVALAGQRLKTEAHIVMPRTTPQIKIDAVEDLGGIVELFGND 173
           KE+L+KGVIC+SAGNHAQGVAL+ QRL  +A IVMP TTP IK  +V+ LG  V L G+ 
Sbjct: 167 KEQLEKGVICSSAGNHAQGVALSAQRLGCDAVIVMPVTTPDIKWKSVKRLGATVVLVGDS 226

Query: 174 FNEAQKRAQERSKEEDLTIIPPFDDEDVIAGQGTIGMEIGRQIRGKIHAIFVPVGGGGLA 233
           ++EAQ  A++R++ E  T IPPFD  DVI GQGT+GMEI RQ++  IHAIFVPVGGGGL 
Sbjct: 227 YDEAQAYAKKRAESEGRTFIPPFDHPDVIVGQGTVGMEINRQLKDNIHAIFVPVGGGGLI 286

Query: 234 AGIVSFYKLVYPDVKVYGVEPNDQNSMALALHLGKVVFVGDIGNFADGVAVRQVGNETFR 293
           AGI ++ K V PD+K+ GVEP D N++AL+LH G+ V +  +G FADGVAV+ VG ET+R
Sbjct: 287 AGIAAYLKRVAPDIKIIGVEPLDANALALSLHHGQRVMLDQVGGFADGVAVKVVGEETYR 346

Query: 294 ICNELLDGVILVKKESIASALKDMFNDGRNILEPSGALSIAGAVAYCRRHGIRRENVVAV 353
           +C EL+DGV+LV +++I +++KDMF + R+ILEP+GAL++AGA AYC+ +G++ ENVVA+
Sbjct: 347 LCEELIDGVVLVGRDAICASIKDMFEEKRSILEPAGALALAGAEAYCKYYGLKGENVVAI 406

Query: 354 TSGANMNFDQL---GHIADLANSDQSILASWLSECPNSLEPLTRVVDRMHFNITEMTYRF 413
           TSGANMNFD+L     +AD+    +++LA+++ E P S +    +V  M  NITE  YR+
Sbjct: 407 TSGANMNFDRLRLVTELADVGRQREAVLATFMPEDPGSFKKFAEMVGPM--NITEFKYRY 466

Query: 414 SSGSVDALLVYKVRPMKKYLEIETLVAELISCGFKTYTLSDNEVVRNHLRFMVGGRQNVE 473
           +S    AL++Y V  +   LE+E +V  + S   +T  L+DN++V++HLR ++GGR NV 
Sbjct: 467 NSDKERALVLYSV-GLHTILELEGMVERMESADLQTINLTDNDLVKDHLRHLMGGRTNVH 526

Query: 474 NETLFRFTFPEKAGALNHFLKGFQPNWNISLLHHRDQGIITSQVLIGIQLEKSEEKKFDE 533
           NE L RFTFPEK GAL  FL  F P WNISL H+R QG   + VL+GIQ+   E  +F+ 
Sbjct: 527 NELLCRFTFPEKPGALMKFLDAFSPRWNISLFHYRAQGDTGANVLVGIQVPPDEVVEFEG 586

Query: 534 YAKEVGYQYEAISPDDSAHVL 552
            A  +GY+Y   S +++  ++
Sbjct: 587 RADSLGYEYAMESLNEAYQLI 604

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BLAST of CmoCh03G011860 vs. ExPASy Swiss-Prot
Match: Q9ZSS6 (Threonine dehydratase biosynthetic, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OMR1 PE=1 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 3.9e-147
Identity = 276/537 (51.40%), Postives = 373/537 (69.46%), Query Frame = 0


Query: 13  GSVAAVPEKNKGHSWKDLEVEEGVIVERKFPPAKPDTKRQMEYLQKILGSNVYDVAIETP 72
           G + AVPE+         E E G I E             MEYL  IL + VYD+AIE+P
Sbjct: 72  GYLGAVPERTN-------EAENGSIAE------------AMEYLTNILSTKVYDIAIESP 131

Query: 73  LQLAPLLSTRMGVNLWVKREDAQQVFSFKIRGAYNMISLLPKEKLKKGVICASAGNHAQG 132
           LQLA  LS R+GV +++KRED Q VFSFK+RGAYNM+  LP ++L KGVIC+SAGNHAQG
Sbjct: 132 LQLAKKLSKRLGVRMYLKREDLQPVFSFKLRGAYNMMVKLPADQLAKGVICSSAGNHAQG 191

Query: 133 VALAGQRLKTEAHIVMPRTTPQIKIDAVEDLGGIVELFGNDFNEAQKRAQERSKEEDLTI 192
           VAL+  +L   A IVMP TTP+IK  AVE+LG  V LFG+ +++AQ  A+ R++EE LT 
Sbjct: 192 VALSASKLGCTAVIVMPVTTPEIKWQAVENLGATVVLFGDSYDQAQAHAKIRAEEEGLTF 251

Query: 193 IPPFDDEDVIAGQGTIGMEIGRQIRGKIHAIFVPVGGGGLAAGIVSFYKLVYPDVKVYGV 252
           IPPFD  DVIAGQGT+GMEI RQ +G +HAIFVPVGGGGL AGI ++ K V P+VK+ GV
Sbjct: 252 IPPFDHPDVIAGQGTVGMEITRQAKGPLHAIFVPVGGGGLIAGIAAYVKRVSPEVKIIGV 311

Query: 253 EPNDQNSMALALHLGKVVFVGDIGNFADGVAVRQVGNETFRICNELLDGVILVKKESIAS 312
           EP D N+MAL+LH G+ V +  +G FADGVAV++VG ETFRI   L+DGV+LV +++I +
Sbjct: 312 EPADANAMALSLHHGERVILDQVGGFADGVAVKEVGEETFRISRNLMDGVVLVTRDAICA 371

Query: 313 ALKDMFNDGRNILEPSGALSIAGAVAYCRRHGIRRENVVAVTSGANMNFDQLGHIADLAN 372
           ++KDMF + RNILEP+GAL++AGA AYC+ +G++  NVVA+TSGANMNFD+L  + +LAN
Sbjct: 372 SIKDMFEEKRNILEPAGALALAGAEAYCKYYGLKDVNVVAITSGANMNFDKLRIVTELAN 431

Query: 373 ---SDQSILASWLSECPNSLEPLTRVVDRMHFNITEMTYRFSSGSVDALLVYKVRPMKKY 432
                +++LA+ + E P S +    +V  M  NI+E  YR SS   +A+++Y V  +   
Sbjct: 432 VGRQQEAVLATLMPEKPGSFKQFCELVGPM--NISEFKYRCSS-EKEAVVLYSV-GVHTA 491

Query: 433 LEIETLVAELISCGFKTYTLSDNEVVRNHLRFMVGGRQNVENETLFRFTFPEKAGALNHF 492
            E++ L   + S   KT  L+ +++V++HLR+++GGR  V +E L RFTFPE+ GAL +F
Sbjct: 492 GELKALQKRMESSQLKTVNLTTSDLVKDHLRYLMGGRSTVGDEVLCRFTFPERPGALMNF 551

Query: 493 LKGFQPNWNISLLHHRDQGIITSQVLIGIQLEKSEEKKFDEYAKEVGYQYEAISPDD 547
           L  F P WNI+L H+R QG   + VL+GIQ+ + E ++F   AK +GY Y  +S DD
Sbjct: 552 LDSFSPRWNITLFHYRGQGETGANVLVGIQVPEQEMEEFKNRAKALGYDYFLVSDDD 585

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BLAST of CmoCh03G011860 vs. ExPASy Swiss-Prot
Match: Q9AXU4 (Threonine dehydratase OS=Nicotiana attenuata OX=49451 GN=TD PE=1 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 5.8e-143
Identity = 262/524 (50.00%), Postives = 369/524 (70.42%), Query Frame = 0


Query: 31  EVEEGVIVERKFPPAKPDTKRQMEYLQKILGSNVYDVAIETPLQLAPLLSTRMGVNLWVK 90
           +VE G ++E    P+  DT+   +YL +IL S VYDVAI++PLQ A  LS ++GVN W+K
Sbjct: 82  DVEAGKLIENN--PSGGDTEELFQYLVEILASRVYDVAIDSPLQNAAKLSKKLGVNFWIK 141

Query: 91  REDAQQVFSFKIRGAYNMISLLPKEKLKKGVICASAGNHAQGVALAGQRLKTEAHIVMPR 150
           RED Q VFSFK+RGAYNM++ L KE+L++GVI ASAGNHAQGVAL  QRLK  A IVMP 
Sbjct: 142 REDMQSVFSFKLRGAYNMMTKLSKEQLERGVITASAGNHAQGVALGAQRLKCTATIVMPV 201

Query: 151 TTPQIKIDAVEDLGGIVELFGNDFNEAQKRAQERSKEEDLTIIPPFDDEDVIAGQGTIGM 210
           TTP+IKI+AV++L G V L G+ F++AQ+ A + +++E LT IPPFD  DVI GQGTIG 
Sbjct: 202 TTPEIKIEAVKNLDGKVVLHGDTFDKAQEHALKLAEDEGLTFIPPFDHPDVIIGQGTIGT 261

Query: 211 EIGRQIRGKIHAIFVPVGGGGLAAGIVSFYKLVYPDVKVYGVEPNDQNSMALALHLGKVV 270
           EI RQ++  IHA+FVPVGGGGL AG+ +++K V P  K+ GVEP   +SM  +L+ G+ V
Sbjct: 262 EINRQLK-DIHAVFVPVGGGGLIAGVAAYFKRVAPHTKIIGVEPFGASSMTQSLYHGERV 321

Query: 271 FVGDIGNFADGVAVRQVGNETFRICNELLDGVILVKKESIASALKDMFNDGRNILEPSGA 330
            +  + NFADGVAV  VG ETFR+C +L+DG++LV  ++I++A+KD++++GRNILE SGA
Sbjct: 322 KLEQVDNFADGVAVALVGEETFRLCKDLIDGMVLVSNDAISAAVKDVYDEGRNILETSGA 381

Query: 331 LSIAGAVAYCRRHGIRRENVVAVTSGANMNFDQLGHIADLAN---SDQSILASWLSECPN 390
           L+IAGA AYC+ + I+ ENVVA+ SGANM+F +L  + DLA+     +++LA+++ E P 
Sbjct: 382 LAIAGAEAYCKYYNIKGENVVAIASGANMDFSKLKLVVDLADIGGQREALLATFMPEEPG 441

Query: 391 SLEPLTRVVDRMHFNITEMTYRFSSGSVDALLVYKVRPMKKYLEIETLVAELISCGFKTY 450
           S +    +V  M  NITE  YR++SG   AL++Y V    K  ++E+++  + S    T 
Sbjct: 442 SFKKFCELVGPM--NITEFKYRYNSGRKQALVLYSVGVNTK-SDLESMLERMKSSQLNTV 501

Query: 451 TLSDNEVVRNHLRFMVGGRQNVENETLFRFTFPEKAGALNHFLKGFQPNWNISLLHHRDQ 510
            L++N +V+ HLR ++GGR    NE   +F FPEK GAL  FL  F P WNISL H+R+Q
Sbjct: 502 NLTNNNLVKEHLRHLMGGRSEPSNEIFCQFIFPEKPGALRKFLDAFSPRWNISLFHYREQ 561

Query: 511 GIITSQVLIGIQLEKSEEKKFDEYAKEVGYQYEAISPDDSAHVL 552
           G + + VL+G Q+ K E ++F   A  +GY YE  S ++++ ++
Sbjct: 562 GELDASVLVGFQVPKGEIEEFRVQANNLGYSYEIESLNEASQLI 599

[Close]

BLAST of CmoCh03G011860 vs. ExPASy Swiss-Prot
Match: P25306 (Threonine dehydratase 2 biosynthetic, chloroplastic OS=Solanum lycopersicum OX=4081 GN=TD2 PE=1 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 1.1e-136
Identity = 256/513 (49.90%), Postives = 353/513 (68.81%), Query Frame = 0


Query: 31  EVEEGVIVERKFPPAKPDTKRQMEYLQKILGSNVYDVAIETPLQLAPLLSTRMGVNLWVK 90
           +V  G ++  K  P   D+    +YL  IL S VYDVAIE+PL+LA  LS R+GVN ++K
Sbjct: 75  KVVPGELIVNK--PTGGDSDELFQYLVDILASPVYDVAIESPLELAEKLSDRLGVNFYIK 134

Query: 91  REDAQQVFSFKIRGAYNMISLLPKEKLKKGVICASAGNHAQGVALAGQRLKTEAHIVMPR 150
           RED Q+VFSFK+RGAYNM+S L +E+L KGVI ASAGNHAQGVALAGQRL   A IVMP 
Sbjct: 135 REDKQRVFSFKLRGAYNMMSNLSREELDKGVITASAGNHAQGVALAGQRLNCVAKIVMPT 194

Query: 151 TTPQIKIDAVEDLGGIVELFGNDFNEAQKRAQERSKEEDLTIIPPFDDEDVIAGQGTIGM 210
           TTPQIKIDAV  LGG V L+G  F+EAQ  A E S+++ L  IPPFDD  VI GQGTIG 
Sbjct: 195 TTPQIKIDAVRALGGDVVLYGKTFDEAQTHALELSEKDGLKYIPPFDDPGVIKGQGTIGT 254

Query: 211 EIGRQIRGKIHAIFVPVGGGGLAAGIVSFYKLVYPDVKVYGVEPNDQNSMALALHLGKVV 270
           EI RQ++  IHA+F+PVGGGGL AG+ +F+K + P+ K+ GVEP    SM L+LH G  V
Sbjct: 255 EINRQLK-DIHAVFIPVGGGGLIAGVATFFKQIAPNTKIIGVEPYGAASMTLSLHEGHRV 314

Query: 271 FVGDIGNFADGVAVRQVGNETFRICNELLDGVILVKKESIASALKDMFNDGRNILEPSGA 330
            + ++  FADGVAV  VG  TF  C EL+DG++LV  + I++A+KD++++GRNILE SGA
Sbjct: 315 KLSNVDTFADGVAVALVGEYTFAKCQELIDGMVLVANDGISAAIKDVYDEGRNILETSGA 374

Query: 331 LSIAGAVAYCRRHGIRRENVVAVTSGANMNFDQLGHIADLA---NSDQSILASWLSECPN 390
           ++IAGA AYC  + I+ EN+VA+ SGANM+F +L  + +LA   +  +++LA+++ E   
Sbjct: 375 VAIAGAAAYCEFYKIKNENIVAIASGANMDFSKLHKVTELAGLGSGKEALLATFMVEQQG 434

Query: 391 SLEPLTRVVDRMHFNITEMTYRFSSGSVDALLVYKVRPMKKYLEIETLVAELISCGFKTY 450
           S +    +V  ++F  TE+TYRF+S   +AL++Y+V  + K  ++E ++ ++ S    T 
Sbjct: 435 SFKTFVGLVGSLNF--TELTYRFTSERKNALILYRVN-VDKESDLEKMIEDMKSSNMTTL 494

Query: 451 TLSDNEVVRNHLRFMVGGRQNVENETLFRFTFPEKAGALNHFLKGFQPNWNISLLHHRDQ 510
            LS NE+V +HL+ +VGG  N+ +E    F  PEKA  L  FL  F P WNI+L  +R+Q
Sbjct: 495 NLSHNELVVDHLKHLVGGSANISDEIFGEFIVPEKAETLKTFLDAFSPRWNITLCRYRNQ 554

Query: 511 GIITSQVLIGIQLEKSEEKKFDEYAKEVGYQYE 541
           G I + +L+G Q+ ++E  +F   A ++GY YE
Sbjct: 555 GDINASLLMGFQVPQAEMDEFKNQADKLGYPYE 581

[Close]

BLAST of CmoCh03G011860 vs. ExPASy Swiss-Prot
Match: P53607 (L-threonine dehydratase biosynthetic IlvA OS=Burkholderia multivorans (strain ATCC 17616 / 249) OX=395019 GN=ilvA PE=3 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 9.9e-119
Identity = 230/494 (46.56%), Postives = 328/494 (66.40%), Query Frame = 0


Query: 54  EYLQKILGSNVYDVAIETPLQLAPLLSTRMGVNLWVKREDAQQVFSFKIRGAYNMISLLP 113
           +YL+KIL + VYDVA ET L+ A  LS R+   +++KRED Q VFSFK+RGAYN ++ +P
Sbjct: 5   DYLKKILTARVYDVAFETELEPARNLSARLRNPVYLKREDNQPVFSFKLRGAYNKMAHIP 64

Query: 114 KEKLKKGVICASAGNHAQGVALAGQRLKTEAHIVMPRTTPQIKIDAVEDLGGI-VELF-- 173
            + L +GVI ASAGNHAQGVA +  R+  +A IV+P TTPQ+K+DAV   GG  VE+   
Sbjct: 65  ADALARGVITASAGNHAQGVAFSAARMGVKAVIVVPVTTPQVKVDAVRAHGGPGVEVIQA 124

Query: 174 GNDFNEAQKRAQERSKEEDLTIIPPFDDEDVIAGQGTIGMEIGRQIRGKIHAIFVPVGGG 233
           G  +++A   A +  +E  LT + PFDD  VIAGQGTI MEI RQ +G IHAIFVP+GGG
Sbjct: 125 GESYSDAYAHALKVQEERGLTFVHPFDDPYVIAGQGTIAMEILRQHQGPIHAIFVPIGGG 184

Query: 234 GLAAGIVSFYKLVYPDVKVYGVEPNDQNSMALALHLGKVVFVGDIGNFADGVAVRQVGNE 293
           GLAAG+ ++ K V P++KV GV+  D  +MA +L  GK V + ++G FADG AV+ VG E
Sbjct: 185 GLAAGVAAYVKAVRPEIKVIGVQAEDSCAMAQSLQAGKRVELAEVGLFADGTAVKLVGEE 244

Query: 294 TFRICNELLDGVILVKKESIASALKDMFNDGRNILEPSGALSIAGAVAYCRRHGIRRENV 353
           TFR+C E LDGV+ V  +++ +A+KD+F D R++LEPSGAL++AGA  Y  R GI  + +
Sbjct: 245 TFRLCKEYLDGVVTVDTDALCAAIKDVFQDTRSVLEPSGALAVAGAKLYAEREGIENQTL 304

Query: 354 VAVTSGANMNFDQLGHIADLA---NSDQSILASWLSECPNSLEPLTRVV-DRMHFNITEM 413
           VAVTSGANMNFD++  +A+ A    + +++ A  + E   S +    +V DR   N+TE 
Sbjct: 305 VAVTSGANMNFDRMRFVAERAEVGEAREAVFAVTIPEERGSFKRFCSLVGDR---NVTEF 364

Query: 414 TYRFSSGSVDALLVYKVRPMKKYLEIETLVAELISCGFKTYTLSDNEVVRNHLRFMVGGR 473
            YR +     +  ++    +++  E   + A   S GFKT  L+ +E+ + H+R+MVGGR
Sbjct: 365 NYRIA--DAQSAHIFVGVQIRRRGESADIAANFESHGFKTADLTHDELSKEHIRYMVGGR 424

Query: 474 QNVE-NETLFRFTFPEKAGALNHFLKGFQPNWNISLLHHRDQGIITSQVLIGIQLEKSEE 533
             +  +E LFRF FPE+ GAL  FL    P+WNISL H+R+QG   S +L+G+Q+ +++ 
Sbjct: 425 SPLALDERLFRFEFPERPGALMKFLSSMAPDWNISLFHYRNQGADYSSILVGLQVPQADH 484

Query: 534 KKFDEYAKEVGYQY 540
            +F+ +   +GY Y
Sbjct: 485 AEFERFLAALGYPY 493

[Close]

BLAST of CmoCh03G011860 vs. ExPASy TrEMBL
Match: A0A6J1EGE8 (Threonine dehydratase OS=Cucurbita moschata OX=3662 GN=LOC111433137 PE=3 SV=1)

HSP 1 Score: 1103.6 bits (2853), Expect = 0.0e+00
Identity = 554/554 (100.00%), Postives = 554/554 (100.00%), Query Frame = 0


Query: 1   MASSFPLNVAADGSVAAVPEKNKGHSWKDLEVEEGVIVERKFPPAKPDTKRQMEYLQKIL 60
           MASSFPLNVAADGSVAAVPEKNKGHSWKDLEVEEGVIVERKFPPAKPDTKRQMEYLQKIL
Sbjct: 1   MASSFPLNVAADGSVAAVPEKNKGHSWKDLEVEEGVIVERKFPPAKPDTKRQMEYLQKIL 60

Query: 61  GSNVYDVAIETPLQLAPLLSTRMGVNLWVKREDAQQVFSFKIRGAYNMISLLPKEKLKKG 120
           GSNVYDVAIETPLQLAPLLSTRMGVNLWVKREDAQQVFSFKIRGAYNMISLLPKEKLKKG
Sbjct: 61  GSNVYDVAIETPLQLAPLLSTRMGVNLWVKREDAQQVFSFKIRGAYNMISLLPKEKLKKG 120

Query: 121 VICASAGNHAQGVALAGQRLKTEAHIVMPRTTPQIKIDAVEDLGGIVELFGNDFNEAQKR 180
           VICASAGNHAQGVALAGQRLKTEAHIVMPRTTPQIKIDAVEDLGGIVELFGNDFNEAQKR
Sbjct: 121 VICASAGNHAQGVALAGQRLKTEAHIVMPRTTPQIKIDAVEDLGGIVELFGNDFNEAQKR 180

Query: 181 AQERSKEEDLTIIPPFDDEDVIAGQGTIGMEIGRQIRGKIHAIFVPVGGGGLAAGIVSFY 240
           AQERSKEEDLTIIPPFDDEDVIAGQGTIGMEIGRQIRGKIHAIFVPVGGGGLAAGIVSFY
Sbjct: 181 AQERSKEEDLTIIPPFDDEDVIAGQGTIGMEIGRQIRGKIHAIFVPVGGGGLAAGIVSFY 240

Query: 241 KLVYPDVKVYGVEPNDQNSMALALHLGKVVFVGDIGNFADGVAVRQVGNETFRICNELLD 300
           KLVYPDVKVYGVEPNDQNSMALALHLGKVVFVGDIGNFADGVAVRQVGNETFRICNELLD
Sbjct: 241 KLVYPDVKVYGVEPNDQNSMALALHLGKVVFVGDIGNFADGVAVRQVGNETFRICNELLD 300

Query: 301 GVILVKKESIASALKDMFNDGRNILEPSGALSIAGAVAYCRRHGIRRENVVAVTSGANMN 360
           GVILVKKESIASALKDMFNDGRNILEPSGALSIAGAVAYCRRHGIRRENVVAVTSGANMN
Sbjct: 301 GVILVKKESIASALKDMFNDGRNILEPSGALSIAGAVAYCRRHGIRRENVVAVTSGANMN 360

Query: 361 FDQLGHIADLANSDQSILASWLSECPNSLEPLTRVVDRMHFNITEMTYRFSSGSVDALLV 420
           FDQLGHIADLANSDQSILASWLSECPNSLEPLTRVVDRMHFNITEMTYRFSSGSVDALLV
Sbjct: 361 FDQLGHIADLANSDQSILASWLSECPNSLEPLTRVVDRMHFNITEMTYRFSSGSVDALLV 420

Query: 421 YKVRPMKKYLEIETLVAELISCGFKTYTLSDNEVVRNHLRFMVGGRQNVENETLFRFTFP 480
           YKVRPMKKYLEIETLVAELISCGFKTYTLSDNEVVRNHLRFMVGGRQNVENETLFRFTFP
Sbjct: 421 YKVRPMKKYLEIETLVAELISCGFKTYTLSDNEVVRNHLRFMVGGRQNVENETLFRFTFP 480

Query: 481 EKAGALNHFLKGFQPNWNISLLHHRDQGIITSQVLIGIQLEKSEEKKFDEYAKEVGYQYE 540
           EKAGALNHFLKGFQPNWNISLLHHRDQGIITSQVLIGIQLEKSEEKKFDEYAKEVGYQYE
Sbjct: 481 EKAGALNHFLKGFQPNWNISLLHHRDQGIITSQVLIGIQLEKSEEKKFDEYAKEVGYQYE 540

Query: 541 AISPDDSAHVLAKL 555
           AISPDDSAHVLAKL
Sbjct: 541 AISPDDSAHVLAKL 554

[Close]

BLAST of CmoCh03G011860 vs. ExPASy TrEMBL
Match: A0A6J1IJC6 (Threonine dehydratase OS=Cucurbita maxima OX=3661 GN=LOC111477609 PE=3 SV=1)

HSP 1 Score: 836.6 bits (2160), Expect = 5.8e-239
Identity = 429/547 (78.43%), Postives = 478/547 (87.39%), Query Frame = 0


Query: 8   NVAADGSVAAVPEKNKGHSWKDLEVEEGVIVERKFPPAKPDTKRQMEYLQKILGSNVYDV 67
           NVA DGSV A  E  K  SWK+L+ EEG    R   P  PDT++QMEY+  ILGSNVYDV
Sbjct: 71  NVAVDGSVVA--EGVKDVSWKELQYEEGSTGHRLPLPTDPDTEKQMEYITNILGSNVYDV 130

Query: 68  AIETPLQLAPLLSTRMGVNLWVKREDAQQVFSFKIRGAYNMISLLPKEKLKKGVICASAG 127
           AIETPLQLAPLLST++GVNLW+KRED+QQVFSFKIRGAYNMIS LP+++LKKG+ICASAG
Sbjct: 131 AIETPLQLAPLLSTQLGVNLWLKREDSQQVFSFKIRGAYNMISKLPEKQLKKGIICASAG 190

Query: 128 NHAQGVALAGQRLKTEAHIVMPRTTPQIKIDAVEDLGGIVELFGNDFNEAQKRAQERSKE 187
           NHAQGVAL+GQRLKT+A+IVMP TTPQIKIDAVE LGGIV+L+GN F+EAQKRA+ERS+E
Sbjct: 191 NHAQGVALSGQRLKTKAYIVMPTTTPQIKIDAVERLGGIVDLYGNSFDEAQKRAKERSEE 250

Query: 188 EDLTIIPPFDDEDVIAGQGTIGMEIGRQIRGKIHAIFVPVGGGGLAAGIVSFYKLVYPDV 247
           E LT IPPFDD+DVIAGQGT+GMEIGRQIRGKIHAIFVP+GGGGLA+GIVSFYKLVYPDV
Sbjct: 251 EGLTFIPPFDDKDVIAGQGTVGMEIGRQIRGKIHAIFVPIGGGGLASGIVSFYKLVYPDV 310

Query: 248 KVYGVEPNDQNSMALALHLGKVVFVGDIGNFADGVAVRQVGNETFRICNELLDGVILVKK 307
           KV+GVEPNDQNSMA AL+ G++V V DIG+FADGVAV+QVG+ETFRIC ELLD VILVKK
Sbjct: 311 KVFGVEPNDQNSMAQALYRGEIVNVTDIGHFADGVAVQQVGDETFRICYELLDDVILVKK 370

Query: 308 ESIASALKDMFNDGRNILEPSGALSIAGAVAYCRRHGIRRENVVAVTSGANMNFDQLGHI 367
           ESI++A+KDMFNDGRNILEPSGALSIA A AYC  + I+  NVVAVTSGANMNFDQLG I
Sbjct: 371 ESISAAIKDMFNDGRNILEPSGALSIAAAKAYCEYNNIKGVNVVAVTSGANMNFDQLGQI 430

Query: 368 ADLANSDQSILASWLSECPNSLEPLTRVVDRMHFNITEMTYRFSSGSVDALLVYKVRPMK 427
           +DLAN DQSILA+ L E P SL+ LT ++  +   ITEMTYRFSSGS DAL+VYKV+ + 
Sbjct: 431 SDLANIDQSILATMLPETPGSLKQLTDLIADLGVTITEMTYRFSSGSTDALVVYKVKAVD 490

Query: 428 KYLEIETLVAELISCGFKTYTLSDNEVVRNHLRFMVGGRQNVENETLFRFTFPEKAGALN 487
             L  ETLV +L   GFKTYTLSDNEVVRNHLRFM+GGRQNVENETLFRFTFPEKAGALN
Sbjct: 491 SAL--ETLVRQLNFFGFKTYTLSDNEVVRNHLRFMMGGRQNVENETLFRFTFPEKAGALN 550

Query: 488 HFLKGFQPNWNISLLHHRDQGIITSQVLIGIQLEKSEEKKFDEYAKEVGYQYEAISPDDS 547
           HFLKGFQPNWNISLLHH D GIITS VLIGIQLEKS EKKFDEYAK+VGYQYEAISPD S
Sbjct: 551 HFLKGFQPNWNISLLHHPDLGIITSDVLIGIQLEKSLEKKFDEYAKKVGYQYEAISPDGS 610

Query: 548 AHVLAKL 555
           A VLAKL
Sbjct: 611 AQVLAKL 613

[Close]

BLAST of CmoCh03G011860 vs. ExPASy TrEMBL
Match: A0A6J1ECR5 (Threonine dehydratase OS=Cucurbita moschata OX=3662 GN=LOC111433136 PE=3 SV=1)

HSP 1 Score: 834.7 bits (2155), Expect = 2.2e-238
Identity = 425/548 (77.55%), Postives = 476/548 (86.86%), Query Frame = 0


Query: 8   NVAADGSVAAV-PEKNKGHSWKDLEVEEGVIVERKFPPAKPDTKRQMEYLQKILGSNVYD 67
           NVA DGSVAAV  E  K  SWK+L+ EEG    R+  PA PDT++QMEY+  ILGSNVYD
Sbjct: 71  NVAVDGSVAAVGAEAVKDVSWKELQYEEGSTGHRQPLPADPDTEKQMEYITNILGSNVYD 130

Query: 68  VAIETPLQLAPLLSTRMGVNLWVKREDAQQVFSFKIRGAYNMISLLPKEKLKKGVICASA 127
           VAIE+PLQLAPLLST++GVNLW+KRED+QQVFSFKIRGAYNMIS L +EKLKKG+ICASA
Sbjct: 131 VAIESPLQLAPLLSTQLGVNLWLKREDSQQVFSFKIRGAYNMISRLSEEKLKKGIICASA 190

Query: 128 GNHAQGVALAGQRLKTEAHIVMPRTTPQIKIDAVEDLGGIVELFGNDFNEAQKRAQERSK 187
           GNHAQGVALAGQRLKT+A+IVMP TTPQIKIDAVE L GIV+L+GN F+EAQKRA+ERS+
Sbjct: 191 GNHAQGVALAGQRLKTKAYIVMPTTTPQIKIDAVERLDGIVDLYGNSFDEAQKRAKERSE 250

Query: 188 EEDLTIIPPFDDEDVIAGQGTIGMEIGRQIRGKIHAIFVPVGGGGLAAGIVSFYKLVYPD 247
           EE LT IPPFDD+DVIAGQGT+GMEIGRQIRGKIHAIFVP+GGGGLA+GIVSFYKLVYPD
Sbjct: 251 EEGLTFIPPFDDKDVIAGQGTVGMEIGRQIRGKIHAIFVPIGGGGLASGIVSFYKLVYPD 310

Query: 248 VKVYGVEPNDQNSMALALHLGKVVFVGDIGNFADGVAVRQVGNETFRICNELLDGVILVK 307
           VKV+GVEPNDQNSMA AL+ G++V V DIG+FADGVAV+QVG ETFRIC ELLD VILVK
Sbjct: 311 VKVFGVEPNDQNSMAQALYRGEIVNVTDIGHFADGVAVQQVGEETFRICYELLDDVILVK 370

Query: 308 KESIASALKDMFNDGRNILEPSGALSIAGAVAYCRRHGIRRENVVAVTSGANMNFDQLGH 367
           KESI++A+KDMFNDGRNILEPSGALSIAGA AYC  + I+  N+VAVTSGANMNFDQLG 
Sbjct: 371 KESISAAIKDMFNDGRNILEPSGALSIAGAKAYCEYNNIKGVNIVAVTSGANMNFDQLGQ 430

Query: 368 IADLANSDQSILASWLSECPNSLEPLTRVVDRMHFNITEMTYRFSSGSVDALLVYKVRPM 427
           I+DLAN DQSILA+ L E P SL+ LT ++  +   I EMTYRFSSGS DAL+VYKV+ +
Sbjct: 431 ISDLANIDQSILATMLPETPGSLKQLTDLIADLGVTIAEMTYRFSSGSTDALVVYKVKAV 490

Query: 428 KKYLEIETLVAELISCGFKTYTLSDNEVVRNHLRFMVGGRQNVENETLFRFTFPEKAGAL 487
              L  ETLV +L   GFKTYTLSDNEVVRNHLR+M+GGRQNVENETLFRFTFPEK GAL
Sbjct: 491 DAAL--ETLVRQLDFFGFKTYTLSDNEVVRNHLRYMMGGRQNVENETLFRFTFPEKVGAL 550

Query: 488 NHFLKGFQPNWNISLLHHRDQGIITSQVLIGIQLEKSEEKKFDEYAKEVGYQYEAISPDD 547
            HFLK FQPNWN+SL HHR QGI+TS VLIG+QLEKSEEKKFDEYAK VGYQYE IS DD
Sbjct: 551 KHFLKDFQPNWNVSLFHHRHQGILTSDVLIGVQLEKSEEKKFDEYAKRVGYQYEVISQDD 610

Query: 548 SAHVLAKL 555
           SAHVLAKL
Sbjct: 611 SAHVLAKL 616

[Close]

BLAST of CmoCh03G011860 vs. ExPASy TrEMBL
Match: A0A6J1ILR7 (Threonine dehydratase OS=Cucurbita maxima OX=3661 GN=LOC111477609 PE=3 SV=1)

HSP 1 Score: 829.7 bits (2142), Expect = 7.1e-237
Identity = 423/547 (77.33%), Postives = 475/547 (86.84%), Query Frame = 0


Query: 8   NVAADGSVAAVPEKNKGHSWKDLEVEEGVIVERKFPPAKPDTKRQMEYLQKILGSNVYDV 67
           NVA DGSV A  E  K  SWK+L+ EEG    R   P  PDT++QMEY+  ILGSNVYDV
Sbjct: 71  NVAVDGSVVA--EGVKDVSWKELQYEEGSTGHRLPLPTDPDTEKQMEYITNILGSNVYDV 130

Query: 68  AIETPLQLAPLLSTRMGVNLWVKREDAQQVFSFKIRGAYNMISLLPKEKLKKGVICASAG 127
           AIETPLQLAPLLST++GVNLW+KRED+QQVFSFKIRGAYNMIS LP+++LKKG+ICASAG
Sbjct: 131 AIETPLQLAPLLSTQLGVNLWLKREDSQQVFSFKIRGAYNMISKLPEKQLKKGIICASAG 190

Query: 128 NHAQGVALAGQRLKTEAHIVMPRTTPQIKIDAVEDLGGIVELFGNDFNEAQKRAQERSKE 187
           NHAQGVAL+GQRLKT+A+IVMP TTPQIKIDAVE LGGIV+L+GN F+EAQKRA+ERS+E
Sbjct: 191 NHAQGVALSGQRLKTKAYIVMPTTTPQIKIDAVERLGGIVDLYGNSFDEAQKRAKERSEE 250

Query: 188 EDLTIIPPFDDEDVIAGQGTIGMEIGRQIRGKIHAIFVPVGGGGLAAGIVSFYKLVYPDV 247
           E LT IPPFDD+DVIAGQGT+GMEIGRQIRGKIHAIFVP+GGGGLA+GIVSFYKLVYPDV
Sbjct: 251 EGLTFIPPFDDKDVIAGQGTVGMEIGRQIRGKIHAIFVPIGGGGLASGIVSFYKLVYPDV 310

Query: 248 KVYGVEPNDQNSMALALHLGKVVFVGDIGNFADGVAVRQVGNETFRICNELLDGVILVKK 307
           KV+GVEPNDQNSMA AL+ G++V V DIG+FADGVAV+QVG+ETFRIC ELLD VILVKK
Sbjct: 311 KVFGVEPNDQNSMAQALYRGEIVNVTDIGHFADGVAVQQVGDETFRICYELLDDVILVKK 370

Query: 308 ESIASALKDMFNDGRNILEPSGALSIAGAVAYCRRHGIRRENVVAVTSGANMNFDQLGHI 367
           ESI++A+KDMFNDGRNILEPSGALSIA A AYC  + I+  NVVAVTSGANMNFDQLG I
Sbjct: 371 ESISAAIKDMFNDGRNILEPSGALSIAAAKAYCEYNNIKGVNVVAVTSGANMNFDQLGQI 430

Query: 368 ADLANSDQSILASWLSECPNSLEPLTRVVDRMHFNITEMTYRFSSGSVDALLVYKVRPMK 427
           +DLAN DQSILA+ L E P SL+ LT ++  +   ITEMTYRFSSGS DAL+VYKV+ + 
Sbjct: 431 SDLANIDQSILATMLPETPGSLKQLTDLIADLGVTITEMTYRFSSGSTDALVVYKVKAVD 490

Query: 428 KYLEIETLVAELISCGFKTYTLSDNEVVRNHLRFMVGGRQNVENETLFRFTFPEKAGALN 487
             L  ETLV +L   GFKTYTLSDNEVVRNHLRFM+GGRQNVENETLFRFTFPEK GAL 
Sbjct: 491 SAL--ETLVRQLNFFGFKTYTLSDNEVVRNHLRFMMGGRQNVENETLFRFTFPEKVGALK 550

Query: 488 HFLKGFQPNWNISLLHHRDQGIITSQVLIGIQLEKSEEKKFDEYAKEVGYQYEAISPDDS 547
           HFLK FQPNWN+SL HHR QGI+TS VLIG+QLEKSEEKKF EYAK+VGYQYEAIS DDS
Sbjct: 551 HFLKDFQPNWNVSLFHHRHQGILTSDVLIGVQLEKSEEKKFHEYAKKVGYQYEAISQDDS 610

Query: 548 AHVLAKL 555
           A VLAKL
Sbjct: 611 AQVLAKL 613

[Close]

BLAST of CmoCh03G011860 vs. ExPASy TrEMBL
Match: A0A0A0KKC3 (Threonine dehydratase OS=Cucumis sativus OX=3659 GN=Csa_6G448740 PE=3 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 1.3e-201
Identity = 364/547 (66.54%), Postives = 436/547 (79.71%), Query Frame = 0


Query: 8   NVAADGSVAAVPEKNKGHSWKDLEVEEGVIVERKFPPAKPDTKRQMEYLQKILGSNVYDV 67
           NVA D SV +  E  K  SW +++ E G I  R  PPA+ D  +QMEY+ KILGS VYDV
Sbjct: 74  NVAVDCSVVS-EEAVKDVSWTEVQYESGSIGHRAPPPAEVDKDKQMEYITKILGSKVYDV 133

Query: 68  AIETPLQLAPLLSTRMGVNLWVKREDAQQVFSFKIRGAYNMISLLPKEKLKKGVICASAG 127
           A+ETPL+LAPLLST++GVNL++KRED QQVFSFKIRGAYNMIS L K+ LK GVICASAG
Sbjct: 134 AVETPLELAPLLSTQLGVNLYLKREDGQQVFSFKIRGAYNMISQLSKDDLKNGVICASAG 193

Query: 128 NHAQGVALAGQRLKTEAHIVMPRTTPQIKIDAVEDLGGIVELFGNDFNEAQKRAQERSKE 187
           NHAQGVAL+ QRLK +A IVMP TTP IK+DAV+ LGG V   G+ F+E+QK A+E S  
Sbjct: 194 NHAQGVALSAQRLKIKAQIVMPTTTPTIKVDAVKRLGGTVIQKGDTFDESQKIAKEISCR 253

Query: 188 EDLTIIPPFDDEDVIAGQGTIGMEIGRQIRGKIHAIFVPVGGGGLAAGIVSFYKLVYPDV 247
             +T IPP+D+EDVIAGQGT+GMEIGRQ+RGKIHAIFVP+GGGGLAAGIVSFYKLVYP+V
Sbjct: 254 CGITFIPPYDNEDVIAGQGTVGMEIGRQMRGKIHAIFVPIGGGGLAAGIVSFYKLVYPEV 313

Query: 248 KVYGVEPNDQNSMALALHLGKVVFVGDIGNFADGVAVRQVGNETFRICNELLDGVILVKK 307
           KV+GVEPND+NSMA AL+  ++V V DIG+FADGVAV+QVGNE FRIC ELLD VILV K
Sbjct: 314 KVFGVEPNDENSMAQALYRDEIVNVTDIGHFADGVAVQQVGNENFRICRELLDDVILVTK 373

Query: 308 ESIASALKDMFNDGRNILEPSGALSIAGAVAYCRRHGIRRENVVAVTSGANMNFDQLGHI 367
           E I++A+KDMF+D R+ILEPSGAL+IA A AYC+ + +   N+VAV SGANMNFDQL  I
Sbjct: 374 EEISAAIKDMFSDERSILEPSGALAIAAARAYCKYNNVTGVNLVAVCSGANMNFDQLREI 433

Query: 368 ADLANSDQSILASWLSECPNSLEPLTRVVDRMHFNITEMTYRFSSGSVDALLVYKVRPMK 427
           +D+AN DQSI+ + L E P SL+ LT ++     NITEM YRFSSGS DA++VYKV  + 
Sbjct: 434 SDIANVDQSIICTMLPETPGSLKELTELLGE---NITEMQYRFSSGSEDAVVVYKVSAVG 493

Query: 428 KYLEIETLVAELISCGFKTYTLSDNEVVRNHLRFMVGGRQNVENETLFRFTFPEKAGALN 487
           K  E+E +V  L S GF TYTL+DN  V+NHLR+M GGR ++ENETLFRFTFPE+ GAL 
Sbjct: 494 K--ELEAMVELLNSAGFTTYTLNDNPTVKNHLRYMTGGRADIENETLFRFTFPERKGALK 553

Query: 488 HFLKGFQPNWNISLLHHRDQGIITSQVLIGIQLEKSEEKKFDEYAKEVGYQYEAISPDDS 547
           HFLK F+P WN+SL HHR QGI+TS VLIG+QLEKSE K F EY  +VGY+YE +  DD+
Sbjct: 554 HFLKDFKPTWNVSLFHHRSQGILTSDVLIGVQLEKSEHKHFHEYINKVGYKYEVVPQDDA 613

Query: 548 AHVLAKL 555
           AHVLAKL
Sbjct: 614 AHVLAKL 614

[Close]

BLAST of CmoCh03G011860 vs. NCBI nr
Match: XP_022925853.1 (threonine dehydratase biosynthetic, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1103.6 bits (2853), Expect = 0.0e+00
Identity = 554/554 (100.00%), Postives = 554/554 (100.00%), Query Frame = 0


Query: 1   MASSFPLNVAADGSVAAVPEKNKGHSWKDLEVEEGVIVERKFPPAKPDTKRQMEYLQKIL 60
           MASSFPLNVAADGSVAAVPEKNKGHSWKDLEVEEGVIVERKFPPAKPDTKRQMEYLQKIL
Sbjct: 1   MASSFPLNVAADGSVAAVPEKNKGHSWKDLEVEEGVIVERKFPPAKPDTKRQMEYLQKIL 60

Query: 61  GSNVYDVAIETPLQLAPLLSTRMGVNLWVKREDAQQVFSFKIRGAYNMISLLPKEKLKKG 120
           GSNVYDVAIETPLQLAPLLSTRMGVNLWVKREDAQQVFSFKIRGAYNMISLLPKEKLKKG
Sbjct: 61  GSNVYDVAIETPLQLAPLLSTRMGVNLWVKREDAQQVFSFKIRGAYNMISLLPKEKLKKG 120

Query: 121 VICASAGNHAQGVALAGQRLKTEAHIVMPRTTPQIKIDAVEDLGGIVELFGNDFNEAQKR 180
           VICASAGNHAQGVALAGQRLKTEAHIVMPRTTPQIKIDAVEDLGGIVELFGNDFNEAQKR
Sbjct: 121 VICASAGNHAQGVALAGQRLKTEAHIVMPRTTPQIKIDAVEDLGGIVELFGNDFNEAQKR 180

Query: 181 AQERSKEEDLTIIPPFDDEDVIAGQGTIGMEIGRQIRGKIHAIFVPVGGGGLAAGIVSFY 240
           AQERSKEEDLTIIPPFDDEDVIAGQGTIGMEIGRQIRGKIHAIFVPVGGGGLAAGIVSFY
Sbjct: 181 AQERSKEEDLTIIPPFDDEDVIAGQGTIGMEIGRQIRGKIHAIFVPVGGGGLAAGIVSFY 240

Query: 241 KLVYPDVKVYGVEPNDQNSMALALHLGKVVFVGDIGNFADGVAVRQVGNETFRICNELLD 300
           KLVYPDVKVYGVEPNDQNSMALALHLGKVVFVGDIGNFADGVAVRQVGNETFRICNELLD
Sbjct: 241 KLVYPDVKVYGVEPNDQNSMALALHLGKVVFVGDIGNFADGVAVRQVGNETFRICNELLD 300

Query: 301 GVILVKKESIASALKDMFNDGRNILEPSGALSIAGAVAYCRRHGIRRENVVAVTSGANMN 360
           GVILVKKESIASALKDMFNDGRNILEPSGALSIAGAVAYCRRHGIRRENVVAVTSGANMN
Sbjct: 301 GVILVKKESIASALKDMFNDGRNILEPSGALSIAGAVAYCRRHGIRRENVVAVTSGANMN 360

Query: 361 FDQLGHIADLANSDQSILASWLSECPNSLEPLTRVVDRMHFNITEMTYRFSSGSVDALLV 420
           FDQLGHIADLANSDQSILASWLSECPNSLEPLTRVVDRMHFNITEMTYRFSSGSVDALLV
Sbjct: 361 FDQLGHIADLANSDQSILASWLSECPNSLEPLTRVVDRMHFNITEMTYRFSSGSVDALLV 420

Query: 421 YKVRPMKKYLEIETLVAELISCGFKTYTLSDNEVVRNHLRFMVGGRQNVENETLFRFTFP 480
           YKVRPMKKYLEIETLVAELISCGFKTYTLSDNEVVRNHLRFMVGGRQNVENETLFRFTFP
Sbjct: 421 YKVRPMKKYLEIETLVAELISCGFKTYTLSDNEVVRNHLRFMVGGRQNVENETLFRFTFP 480

Query: 481 EKAGALNHFLKGFQPNWNISLLHHRDQGIITSQVLIGIQLEKSEEKKFDEYAKEVGYQYE 540
           EKAGALNHFLKGFQPNWNISLLHHRDQGIITSQVLIGIQLEKSEEKKFDEYAKEVGYQYE
Sbjct: 481 EKAGALNHFLKGFQPNWNISLLHHRDQGIITSQVLIGIQLEKSEEKKFDEYAKEVGYQYE 540

Query: 541 AISPDDSAHVLAKL 555
           AISPDDSAHVLAKL
Sbjct: 541 AISPDDSAHVLAKL 554

[Close]

BLAST of CmoCh03G011860 vs. NCBI nr
Match: XP_023543122.1 (threonine dehydratase biosynthetic, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1025.0 bits (2649), Expect = 2.4e-295
Identity = 515/540 (95.37%), Postives = 527/540 (97.59%), Query Frame = 0


Query: 15  VAAVPEKNKGHSWKDLEVEEGVIVERKFPPAKPDTKRQMEYLQKILGSNVYDVAIETPLQ 74
           VAAVPE++KGHSWKDLEVEEGVIVERKFPPA PDTKRQMEYLQKILGSNVYDVAIETPLQ
Sbjct: 2   VAAVPEEDKGHSWKDLEVEEGVIVERKFPPANPDTKRQMEYLQKILGSNVYDVAIETPLQ 61

Query: 75  LAPLLSTRMGVNLWVKREDAQQVFSFKIRGAYNMISLLPKEKLKKGVICASAGNHAQGVA 134
           LAPLLSTRMGVNLWVKREDAQQVFSFKIRGAYNMISLLPK+KLKKGVICASAGNHAQGVA
Sbjct: 62  LAPLLSTRMGVNLWVKREDAQQVFSFKIRGAYNMISLLPKQKLKKGVICASAGNHAQGVA 121

Query: 135 LAGQRLKTEAHIVMPRTTPQIKIDAVEDLGGIVELFGNDFNEAQKRAQERSKEEDLTIIP 194
           LAGQRLKTEAHIVMP TTPQIKIDAVEDLGGIV+LFGNDFNEAQ+RAQERSKEEDLTIIP
Sbjct: 122 LAGQRLKTEAHIVMPTTTPQIKIDAVEDLGGIVDLFGNDFNEAQERAQERSKEEDLTIIP 181

Query: 195 PFDDEDVIAGQGTIGMEIGRQIRGKIHAIFVPVGGGGLAAGIVSFYKLVYPDVKVYGVEP 254
           PFDDEDVIAGQGTIGMEIGRQIRGKIHAIFVPVGGGGLAAGIVSFYKLVYPDVKVYGVEP
Sbjct: 182 PFDDEDVIAGQGTIGMEIGRQIRGKIHAIFVPVGGGGLAAGIVSFYKLVYPDVKVYGVEP 241

Query: 255 NDQNSMALALHLGKVVFVGDIGNFADGVAVRQVGNETFRICNELLDGVILVKKESIASAL 314
           ND NSMALALHLGKVVFVGDIGNFADGVAVRQVGNETFRICNELLDGVILVKKESIASAL
Sbjct: 242 NDHNSMALALHLGKVVFVGDIGNFADGVAVRQVGNETFRICNELLDGVILVKKESIASAL 301

Query: 315 KDMFNDGRNILEPSGALSIAGAVAYCRRHGIRRENVVAVTSGANMNFDQLGHIADLANSD 374
           KDMFNDGRNILEPSGALSIAGAVAYCR HGIRRENVVAVTSGANMNFDQLGHIADLANSD
Sbjct: 302 KDMFNDGRNILEPSGALSIAGAVAYCRYHGIRRENVVAVTSGANMNFDQLGHIADLANSD 361

Query: 375 QSILASWLSECPNSLEPLTRVVDRMHFNITEMTYRFSSGSVDALLVYKVRPMKKYLEIET 434
           QSILASWL ECP SL  LT VV RM F+ITEMTYRFSSGSVDALLVYKVR +K+ L+I+T
Sbjct: 362 QSILASWLPECPKSLGRLTDVVTRMDFSITEMTYRFSSGSVDALLVYKVRAVKEDLKIDT 421

Query: 435 LVAELISCGFKTYTLSDNEVVRNHLRFMVGGRQNVENETLFRFTFPEKAGALNHFLKGFQ 494
           LVAELISCGFKTYTLSDNEVVRNHLRFMVGGRQNVENETLFRFTFPEKAGALNHFL+GFQ
Sbjct: 422 LVAELISCGFKTYTLSDNEVVRNHLRFMVGGRQNVENETLFRFTFPEKAGALNHFLQGFQ 481

Query: 495 PNWNISLLHHRDQGIITSQVLIGIQLEKSEEKKFDEYAKEVGYQYEAISPDDSAHVLAKL 554
           PNWNISLLHHRDQGIITS+VLIGIQLEKSEEKKFDEYAKEVGYQYEAISPDDSAHVLAKL
Sbjct: 482 PNWNISLLHHRDQGIITSEVLIGIQLEKSEEKKFDEYAKEVGYQYEAISPDDSAHVLAKL 541

[Close]

BLAST of CmoCh03G011860 vs. NCBI nr
Match: KAG6604379.1 (Threonine dehydratase biosynthetic, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 842.0 bits (2174), Expect = 2.9e-240
Identity = 443/550 (80.55%), Postives = 460/550 (83.64%), Query Frame = 0


Query: 1   MASSFPLNVAADGSVAAVPEKNKGHSWKDLEVEEGVIVERKFPPAKPDTKRQMEYLQKIL 60
           MASSFPLNVAADGSVA  PE+ K  SWKDLEVEEG +VERK PPA PDTKRQM+ L   L
Sbjct: 1   MASSFPLNVAADGSVATAPEEAKRVSWKDLEVEEGDVVERKSPPANPDTKRQMDLLPSCL 60

Query: 61  GSNVYDVAIETPLQLAPLLSTRMGVNLWVKREDAQQVFSFKIRGAYNMISLLPKEKLKKG 120
            SN                         V +E           GAYNMIS LPKEKLKKG
Sbjct: 61  YSN---------------------GGQSVGQEGGGTA------GAYNMISQLPKEKLKKG 120

Query: 121 VICASAGNHAQGVALAGQRLKTEAHIVMPRTTPQIKIDAVEDLGGIVELFGNDFNEAQKR 180
           VICASAGNHAQGVALAGQRLKTEAHIVMP TTPQIKIDAVEDLGGIV+LFGNDFN+AQ+R
Sbjct: 121 VICASAGNHAQGVALAGQRLKTEAHIVMPTTTPQIKIDAVEDLGGIVDLFGNDFNQAQER 180

Query: 181 AQERSKEEDLTIIPPFDDEDVIAGQGTIGMEIGRQIRGKIHAIFVPVGGGGLAAGIVSFY 240
           A+ERSKEEDLTIIPPFDDEDVIAGQGTIGMEIGRQIRG+IHAIFVPVGGGGLAAGIVSFY
Sbjct: 181 AEERSKEEDLTIIPPFDDEDVIAGQGTIGMEIGRQIRGRIHAIFVPVGGGGLAAGIVSFY 240

Query: 241 KLVYPDVKVYGVEPNDQNSMALALHLGKVVFVGDIGNFADGVAVRQVGNETFRICNELLD 300
           KLVYPDVKV+GVEPNDQNSMA AL+ GKVV+V DIGNFADGVAV+QVGNETFRICNELLD
Sbjct: 241 KLVYPDVKVFGVEPNDQNSMAQALYRGKVVYVHDIGNFADGVAVKQVGNETFRICNELLD 300

Query: 301 GVILVKKESIASALKDMFNDGRNILEPSGALSIAGAVAYCRRHGIRRENVVAVTSGANMN 360
           GVILVKKESIASALK MFNDGRNILEPSGALSIAGAV YCRRHGIRRENVVAVTSGANMN
Sbjct: 301 GVILVKKESIASALKAMFNDGRNILEPSGALSIAGAVEYCRRHGIRRENVVAVTSGANMN 360

Query: 361 FDQLGHIADLANSDQSILASWLSECPNSLEPLTRVVDRMHFNITEMTYRFSSGSVDALLV 420
           FDQLGHIADLANSDQSILASWL ECP SLE LTRVVDRMHFNITEMTYRFSSGSVDALLV
Sbjct: 361 FDQLGHIADLANSDQSILASWLPECPKSLESLTRVVDRMHFNITEMTYRFSSGSVDALLV 420

Query: 421 YKVRPMKKYLEIETLVAELISCGFKTYTLSDNEVVRNHLRFMVGGRQNVENETLFRFTFP 480
           YKVRP+K+ L+IETLVAELISCGFKTYTLSDNEVVRNHL                     
Sbjct: 421 YKVRPVKEDLKIETLVAELISCGFKTYTLSDNEVVRNHLNL------------------- 480

Query: 481 EKAGALNHFLKGFQPNWNISLLHHRDQGIITSQVLIGIQLEKSEEKKFDEYAKEVGYQYE 540
                  HFLKGFQPNWNISLLHHRDQGIITS+VLIGIQLEKSEEKKFDEYAKEVGYQYE
Sbjct: 481 -------HFLKGFQPNWNISLLHHRDQGIITSEVLIGIQLEKSEEKKFDEYAKEVGYQYE 497

Query: 541 AISPDDSAHV 551
            ISPDDSAHV
Sbjct: 541 TISPDDSAHV 497

[Close]

BLAST of CmoCh03G011860 vs. NCBI nr
Match: XP_022977241.1 (threonine dehydratase biosynthetic, chloroplastic-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 836.6 bits (2160), Expect = 1.2e-238
Identity = 429/547 (78.43%), Postives = 478/547 (87.39%), Query Frame = 0


Query: 8   NVAADGSVAAVPEKNKGHSWKDLEVEEGVIVERKFPPAKPDTKRQMEYLQKILGSNVYDV 67
           NVA DGSV A  E  K  SWK+L+ EEG    R   P  PDT++QMEY+  ILGSNVYDV
Sbjct: 71  NVAVDGSVVA--EGVKDVSWKELQYEEGSTGHRLPLPTDPDTEKQMEYITNILGSNVYDV 130

Query: 68  AIETPLQLAPLLSTRMGVNLWVKREDAQQVFSFKIRGAYNMISLLPKEKLKKGVICASAG 127
           AIETPLQLAPLLST++GVNLW+KRED+QQVFSFKIRGAYNMIS LP+++LKKG+ICASAG
Sbjct: 131 AIETPLQLAPLLSTQLGVNLWLKREDSQQVFSFKIRGAYNMISKLPEKQLKKGIICASAG 190

Query: 128 NHAQGVALAGQRLKTEAHIVMPRTTPQIKIDAVEDLGGIVELFGNDFNEAQKRAQERSKE 187
           NHAQGVAL+GQRLKT+A+IVMP TTPQIKIDAVE LGGIV+L+GN F+EAQKRA+ERS+E
Sbjct: 191 NHAQGVALSGQRLKTKAYIVMPTTTPQIKIDAVERLGGIVDLYGNSFDEAQKRAKERSEE 250

Query: 188 EDLTIIPPFDDEDVIAGQGTIGMEIGRQIRGKIHAIFVPVGGGGLAAGIVSFYKLVYPDV 247
           E LT IPPFDD+DVIAGQGT+GMEIGRQIRGKIHAIFVP+GGGGLA+GIVSFYKLVYPDV
Sbjct: 251 EGLTFIPPFDDKDVIAGQGTVGMEIGRQIRGKIHAIFVPIGGGGLASGIVSFYKLVYPDV 310

Query: 248 KVYGVEPNDQNSMALALHLGKVVFVGDIGNFADGVAVRQVGNETFRICNELLDGVILVKK 307
           KV+GVEPNDQNSMA AL+ G++V V DIG+FADGVAV+QVG+ETFRIC ELLD VILVKK
Sbjct: 311 KVFGVEPNDQNSMAQALYRGEIVNVTDIGHFADGVAVQQVGDETFRICYELLDDVILVKK 370

Query: 308 ESIASALKDMFNDGRNILEPSGALSIAGAVAYCRRHGIRRENVVAVTSGANMNFDQLGHI 367
           ESI++A+KDMFNDGRNILEPSGALSIA A AYC  + I+  NVVAVTSGANMNFDQLG I
Sbjct: 371 ESISAAIKDMFNDGRNILEPSGALSIAAAKAYCEYNNIKGVNVVAVTSGANMNFDQLGQI 430

Query: 368 ADLANSDQSILASWLSECPNSLEPLTRVVDRMHFNITEMTYRFSSGSVDALLVYKVRPMK 427
           +DLAN DQSILA+ L E P SL+ LT ++  +   ITEMTYRFSSGS DAL+VYKV+ + 
Sbjct: 431 SDLANIDQSILATMLPETPGSLKQLTDLIADLGVTITEMTYRFSSGSTDALVVYKVKAVD 490

Query: 428 KYLEIETLVAELISCGFKTYTLSDNEVVRNHLRFMVGGRQNVENETLFRFTFPEKAGALN 487
             L  ETLV +L   GFKTYTLSDNEVVRNHLRFM+GGRQNVENETLFRFTFPEKAGALN
Sbjct: 491 SAL--ETLVRQLNFFGFKTYTLSDNEVVRNHLRFMMGGRQNVENETLFRFTFPEKAGALN 550

Query: 488 HFLKGFQPNWNISLLHHRDQGIITSQVLIGIQLEKSEEKKFDEYAKEVGYQYEAISPDDS 547
           HFLKGFQPNWNISLLHH D GIITS VLIGIQLEKS EKKFDEYAK+VGYQYEAISPD S
Sbjct: 551 HFLKGFQPNWNISLLHHPDLGIITSDVLIGIQLEKSLEKKFDEYAKKVGYQYEAISPDGS 610

Query: 548 AHVLAKL 555
           A VLAKL
Sbjct: 611 AQVLAKL 613

[Close]

BLAST of CmoCh03G011860 vs. NCBI nr
Match: KAG6604380.1 (Threonine dehydratase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 835.9 bits (2158), Expect = 2.1e-238
Identity = 426/548 (77.74%), Postives = 476/548 (86.86%), Query Frame = 0


Query: 8   NVAADGSVAAV-PEKNKGHSWKDLEVEEGVIVERKFPPAKPDTKRQMEYLQKILGSNVYD 67
           NVA DGSVAAV  E  K  SWK+L+ EEG    R+  P  PDT++QMEY+ KILGSNVYD
Sbjct: 71  NVAVDGSVAAVAAEGVKDVSWKELQYEEGSTGHRQPLPTNPDTEKQMEYITKILGSNVYD 130

Query: 68  VAIETPLQLAPLLSTRMGVNLWVKREDAQQVFSFKIRGAYNMISLLPKEKLKKGVICASA 127
           VAIETPLQLAPLLST++GVNLW+KRED+QQVFSFKIRGAYNMIS LP+E+LKKG+ICASA
Sbjct: 131 VAIETPLQLAPLLSTQLGVNLWLKREDSQQVFSFKIRGAYNMISQLPEEQLKKGIICASA 190

Query: 128 GNHAQGVALAGQRLKTEAHIVMPRTTPQIKIDAVEDLGGIVELFGNDFNEAQKRAQERSK 187
           GNHAQGVAL+GQRLKT+A+IVMP TTPQIKIDAVE LGGIV+L GN F+EAQKRA+ERS+
Sbjct: 191 GNHAQGVALSGQRLKTKAYIVMPTTTPQIKIDAVERLGGIVDLHGNSFDEAQKRAKERSE 250

Query: 188 EEDLTIIPPFDDEDVIAGQGTIGMEIGRQIRGKIHAIFVPVGGGGLAAGIVSFYKLVYPD 247
           EE LT IPPFDD+DVIAGQGT+GMEIGRQIRGKIHAIFVP+GGGGLA+GIVSFYKLVYPD
Sbjct: 251 EEGLTFIPPFDDKDVIAGQGTVGMEIGRQIRGKIHAIFVPIGGGGLASGIVSFYKLVYPD 310

Query: 248 VKVYGVEPNDQNSMALALHLGKVVFVGDIGNFADGVAVRQVGNETFRICNELLDGVILVK 307
           VKV+GVEPNDQNSMA AL+ G++V V DIG+FADGVAV+QVG ETFRIC ELLD VILVK
Sbjct: 311 VKVFGVEPNDQNSMAQALYRGEIVNVTDIGHFADGVAVQQVGEETFRICYELLDDVILVK 370

Query: 308 KESIASALKDMFNDGRNILEPSGALSIAGAVAYCRRHGIRRENVVAVTSGANMNFDQLGH 367
           KESI++A+KDMFNDGRNILEPSGALSIA A AYC  + I+  NVVAVTSGANMNFDQLG 
Sbjct: 371 KESISAAIKDMFNDGRNILEPSGALSIAAAKAYCEYNNIKGVNVVAVTSGANMNFDQLGQ 430

Query: 368 IADLANSDQSILASWLSECPNSLEPLTRVVDRMHFNITEMTYRFSSGSVDALLVYKVRPM 427
           I+DLAN DQSILA+ L E P SL+ LT ++  +   ITEMTYRFSSGS DAL+VYKV+ +
Sbjct: 431 ISDLANIDQSILATMLPETPGSLKQLTDLIADLGVTITEMTYRFSSGSTDALVVYKVKAV 490

Query: 428 KKYLEIETLVAELISCGFKTYTLSDNEVVRNHLRFMVGGRQNVENETLFRFTFPEKAGAL 487
              L  ETLV +L   GFKTYTLSDNEVVRNHLR+M+GGRQNVENETLFRFTFPEK GAL
Sbjct: 491 DSAL--ETLVRQLNFFGFKTYTLSDNEVVRNHLRYMMGGRQNVENETLFRFTFPEKVGAL 550

Query: 488 NHFLKGFQPNWNISLLHHRDQGIITSQVLIGIQLEKSEEKKFDEYAKEVGYQYEAISPDD 547
            HFLK FQPNWN+SL HHR QGI+TS VLIG+QLEKSEEKKF EYAK VGYQYE IS DD
Sbjct: 551 KHFLKDFQPNWNVSLFHHRHQGILTSDVLIGVQLEKSEEKKFHEYAKRVGYQYEVISQDD 610

Query: 548 SAHVLAKL 555
           SAHVLAKL
Sbjct: 611 SAHVLAKL 616

[Close]

BLAST of CmoCh03G011860 vs. TAIR 10
Match: AT3G10050.1 (L-O-methylthreonine resistant 1 )

HSP 1 Score: 523.1 bits (1346), Expect = 2.8e-148
Identity = 276/537 (51.40%), Postives = 373/537 (69.46%), Query Frame = 0


Query: 13  GSVAAVPEKNKGHSWKDLEVEEGVIVERKFPPAKPDTKRQMEYLQKILGSNVYDVAIETP 72
           G + AVPE+         E E G I E             MEYL  IL + VYD+AIE+P
Sbjct: 72  GYLGAVPERTN-------EAENGSIAE------------AMEYLTNILSTKVYDIAIESP 131

Query: 73  LQLAPLLSTRMGVNLWVKREDAQQVFSFKIRGAYNMISLLPKEKLKKGVICASAGNHAQG 132
           LQLA  LS R+GV +++KRED Q VFSFK+RGAYNM+  LP ++L KGVIC+SAGNHAQG
Sbjct: 132 LQLAKKLSKRLGVRMYLKREDLQPVFSFKLRGAYNMMVKLPADQLAKGVICSSAGNHAQG 191

Query: 133 VALAGQRLKTEAHIVMPRTTPQIKIDAVEDLGGIVELFGNDFNEAQKRAQERSKEEDLTI 192
           VAL+  +L   A IVMP TTP+IK  AVE+LG  V LFG+ +++AQ  A+ R++EE LT 
Sbjct: 192 VALSASKLGCTAVIVMPVTTPEIKWQAVENLGATVVLFGDSYDQAQAHAKIRAEEEGLTF 251

Query: 193 IPPFDDEDVIAGQGTIGMEIGRQIRGKIHAIFVPVGGGGLAAGIVSFYKLVYPDVKVYGV 252
           IPPFD  DVIAGQGT+GMEI RQ +G +HAIFVPVGGGGL AGI ++ K V P+VK+ GV
Sbjct: 252 IPPFDHPDVIAGQGTVGMEITRQAKGPLHAIFVPVGGGGLIAGIAAYVKRVSPEVKIIGV 311

Query: 253 EPNDQNSMALALHLGKVVFVGDIGNFADGVAVRQVGNETFRICNELLDGVILVKKESIAS 312
           EP D N+MAL+LH G+ V +  +G FADGVAV++VG ETFRI   L+DGV+LV +++I +
Sbjct: 312 EPADANAMALSLHHGERVILDQVGGFADGVAVKEVGEETFRISRNLMDGVVLVTRDAICA 371

Query: 313 ALKDMFNDGRNILEPSGALSIAGAVAYCRRHGIRRENVVAVTSGANMNFDQLGHIADLAN 372
           ++KDMF + RNILEP+GAL++AGA AYC+ +G++  NVVA+TSGANMNFD+L  + +LAN
Sbjct: 372 SIKDMFEEKRNILEPAGALALAGAEAYCKYYGLKDVNVVAITSGANMNFDKLRIVTELAN 431

Query: 373 ---SDQSILASWLSECPNSLEPLTRVVDRMHFNITEMTYRFSSGSVDALLVYKVRPMKKY 432
                +++LA+ + E P S +    +V  M  NI+E  YR SS   +A+++Y V  +   
Sbjct: 432 VGRQQEAVLATLMPEKPGSFKQFCELVGPM--NISEFKYRCSS-EKEAVVLYSV-GVHTA 491

Query: 433 LEIETLVAELISCGFKTYTLSDNEVVRNHLRFMVGGRQNVENETLFRFTFPEKAGALNHF 492
            E++ L   + S   KT  L+ +++V++HLR+++GGR  V +E L RFTFPE+ GAL +F
Sbjct: 492 GELKALQKRMESSQLKTVNLTTSDLVKDHLRYLMGGRSTVGDEVLCRFTFPERPGALMNF 551

Query: 493 LKGFQPNWNISLLHHRDQGIITSQVLIGIQLEKSEEKKFDEYAKEVGYQYEAISPDD 547
           L  F P WNI+L H+R QG   + VL+GIQ+ + E ++F   AK +GY Y  +S DD
Sbjct: 552 LDSFSPRWNITLFHYRGQGETGANVLVGIQVPEQEMEEFKNRAKALGYDYFLVSDDD 585

[Close]

BLAST of CmoCh03G011860 vs. TAIR 10
Match: AT4G11640.1 (serine racemase )

HSP 1 Score: 149.4 bits (376), Expect = 8.3e-36
Identity = 92/297 (30.98%), Postives = 162/297 (54.55%), Query Frame = 0


Query: 71  TPLQLAPLLSTRMGVNLWVKREDAQQVFSFKIRGAYNMISLLPKEKLKKGVICASAGNHA 130
           TP+  +  L++  G +L+ K E  Q+  +FK RGA N +  L  E+  KGV+  S+GNHA
Sbjct: 29  TPVLTSESLNSISGRSLFFKCECLQKGGAFKFRGACNAVLSLDAEQAAKGVVTHSSGNHA 88

Query: 131 QGVALAGQRLKTEAHIVMPRTTPQIKIDAVEDLGGIVELFGNDFNEAQKRAQERSKEEDL 190
             ++LA +     A+IV+P+  P+ K+D V   GG V       +  ++ A +  +E   
Sbjct: 89  AALSLAAKIQGIPAYIVVPKGAPKCKVDNVIRYGGKVIWSEATMSSREEIASKVLQETGS 148

Query: 191 TIIPPFDDEDVIAGQGTIGMEIGRQIRGKIHAIFVPVGGGGLAAGIVSFYKLVYPDVKVY 250
            +I P++D  +I+GQGTI +E+  QI+ +I AI VP+ GGGL +G+    K + P +++ 
Sbjct: 149 VLIHPYNDGRIISGQGTIALELLEQIQ-EIDAIVVPISGGGLISGVALAAKSIKPSIRII 208

Query: 251 GVEPNDQNSMALALHLGKVVFVGDIGNFADGVAVRQVGNETFRICNELLDGVILVKKESI 310
             EP   +  A +   GK++ +      ADG+    +G+ T+ +  +L+D V+ +++  I
Sbjct: 209 AAEPKGADDAAQSKVAGKIITLPVTNTIADGLRA-SLGDLTWPVVRDLVDDVVTLEECEI 268

Query: 311 ASALKDMFNDGRNILEPSGALSIAGAVAYCRRHGIR---RENVVAVTSGANMNFDQL 365
             A+K  +   +  +EPSGA+ +A  ++   R+       +N+  V SG N++   L
Sbjct: 269 IEAMKMCYEILKVSVEPSGAIGLAAVLSNSFRNNPSCRDCKNIGIVLSGGNVDLGSL 323

[Close]

BLAST of CmoCh03G011860 vs. TAIR 10
Match: AT3G03630.1 (cysteine synthase 26 )

HSP 1 Score: 52.8 bits (125), Expect = 1.1e-06
Identity = 73/324 (22.53%), Postives = 131/324 (40.43%), Query Frame = 0


Query: 39  ERKFPPAKPDTKRQMEYLQKILGSNVYDVAIE-------TPLQLAPLLSTRMGVNLWVKR 98
           +RK PP  P T    E  Q I  S   ++A +       TP+     ++     ++  K 
Sbjct: 73  KRKPPPPPPVTTVAEE--QHIAESETVNIAEDVTQLIGSTPMVYLNRVTDGCLADIAAKL 132

Query: 99  EDAQQVFSFKIRGAYNMI-------SLLPKEKLKKGVICASAGNHAQGVALAGQRLKTEA 158
           E  +   S K R   +MI       ++ P+   K  ++  + GN   G+A        + 
Sbjct: 133 ESMEPCRSVKDRIGLSMINEAENSGAITPR---KTVLVEPTTGNTGLGIAFVAAAKGYKL 192

Query: 159 HIVMPRTTPQIKIDAVEDLGGIVELFGNDFNEAQKRAQERSKE-----EDLTIIPPFDD- 218
            + MP +    +   +  LG  + L   +  +  K A +++KE     ++  +   FD+ 
Sbjct: 193 IVTMPASINIERRMLLRALGAEIVLTNPE--KGLKGAVDKAKEIVLKTKNAYMFQQFDNT 252

Query: 219 EDVIAGQGTIGMEIGRQIRGKIHAIFVPVGGGGLAAGIVSFYKLVYPDVKVYGVEPNDQN 278
            +      T G EI     G +      +G GG   G   F K++  D+KV GVEP++++
Sbjct: 253 ANTKIHFETTGPEIWEDTMGNVDIFVAGIGTGGTVTGTGGFLKMMNKDIKVVGVEPSERS 312

Query: 279 SMALALHLGKVVFVGDIGNFADGVAVRQVGNETFRICNELLDGVILVKKESIASALKDMF 338
                      V  GD   +  G+   ++ +E F++ N           E+I  A +   
Sbjct: 313 -----------VISGDNPGYLPGILDVKLLDEVFKVSN----------GEAIEMARRLAL 367

Query: 339 NDGRNILEPSGALSIAGAVAYCRR 343
            +G  +   SGA ++A AV+  +R
Sbjct: 373 EEGLLVGISSGAAAVA-AVSLAKR 367

[Close]

BLAST of CmoCh03G011860 vs. TAIR 10
Match: AT3G61440.1 (cysteine synthase C1 )

HSP 1 Score: 46.6 bits (109), Expect = 7.5e-05
Identity = 48/197 (24.37%), Postives = 77/197 (39.09%), Query Frame = 0


Query: 70  ETPLQLAPLLSTRMGVNLWVKREDAQQVFSFKIRGAYNMISLLPKEKL----KKGVICAS 129
           +TPL     ++      +  K+E  Q   S K R A  MI+   K+KL    K  +I  +
Sbjct: 59  KTPLVFLNKVTEGCEAYVAAKQEHFQPTCSIKDRPAIAMIADAEKKKLIIPGKTTLIEPT 118

Query: 130 AGNHAQGVALAGQRLKTEAHIVMPRTTPQIKIDAVEDLGGIVELF--GNDFNEAQKRAQE 189
           +GN    +A           + MP  T   +   +   G  + L           K+A +
Sbjct: 119 SGNMGISLAFMAAMKGYRIIMTMPSYTSLERRVTMRSFGAELVLTDPAKGMGGTVKKAYD 178

Query: 190 -RSKEEDLTIIPPF-DDEDVIAGQGTIGMEIGRQIRGKIHAIFVPVGGGGLAAGIVSFYK 249
                 D  +   F +  +      T G EI     G +    + +G GG  +G+  + K
Sbjct: 179 LLDSTPDAFMCQQFANPANTQIHFDTTGPEIWEDTLGNVDIFVMGIGSGGTVSGVGRYLK 238

Query: 250 LVYPDVKVYGVEPNDQN 259
              P+VK+YGVEP + N
Sbjct: 239 SKNPNVKIYGVEPAESN 255

[Close]

BLAST of CmoCh03G011860 vs. TAIR 10
Match: AT3G61440.2 (cysteine synthase C1 )

HSP 1 Score: 43.1 bits (100), Expect = 8.3e-04
Identity = 19/52 (36.54%), Postives = 28/52 (53.85%), Query Frame = 0


Query: 207 TIGMEIGRQIRGKIHAIFVPVGGGGLAAGIVSFYKLVYPDVKVYGVEPNDQN 259
           T G EI     G +    + +G GG  +G+  + K   P+VK+YGVEP + N
Sbjct: 108 TTGPEIWEDTLGNVDIFVMGIGSGGTVSGVGRYLKSKNPNVKIYGVEPAESN 159

The following BLAST results are available for this feature:

BLAST of CmoCh03G011860 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0FKE6	3.1e-152	54.29	Threonine dehydratase 1 biosynthetic, chloroplastic OS=Solanum lycopersicum OX=4...	[more]
Q9ZSS6	3.9e-147	51.40	Threonine dehydratase biosynthetic, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
Q9AXU4	5.8e-143	50.00	Threonine dehydratase OS=Nicotiana attenuata OX=49451 GN=TD PE=1 SV=1	[more]
P25306	1.1e-136	49.90	Threonine dehydratase 2 biosynthetic, chloroplastic OS=Solanum lycopersicum OX=4...	[more]
P53607	9.9e-119	46.56	L-threonine dehydratase biosynthetic IlvA OS=Burkholderia multivorans (strain AT...	[more]

BLAST of CmoCh03G011860 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EGE8	0.0e+00	100.00	Threonine dehydratase OS=Cucurbita moschata OX=3662 GN=LOC111433137 PE=3 SV=1	[more]
A0A6J1IJC6	5.8e-239	78.43	Threonine dehydratase OS=Cucurbita maxima OX=3661 GN=LOC111477609 PE=3 SV=1	[more]
A0A6J1ECR5	2.2e-238	77.55	Threonine dehydratase OS=Cucurbita moschata OX=3662 GN=LOC111433136 PE=3 SV=1	[more]
A0A6J1ILR7	7.1e-237	77.33	Threonine dehydratase OS=Cucurbita maxima OX=3661 GN=LOC111477609 PE=3 SV=1	[more]
A0A0A0KKC3	1.3e-201	66.54	Threonine dehydratase OS=Cucumis sativus OX=3659 GN=Csa_6G448740 PE=3 SV=1	[more]

BLAST of CmoCh03G011860 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022925853.1	0.0e+00	100.00	threonine dehydratase biosynthetic, chloroplastic-like [Cucurbita moschata]	[more]
XP_023543122.1	2.4e-295	95.37	threonine dehydratase biosynthetic, chloroplastic-like [Cucurbita pepo subsp. pe...	[more]
KAG6604379.1	2.9e-240	80.55	Threonine dehydratase biosynthetic, chloroplastic, partial [Cucurbita argyrosper...	[more]
XP_022977241.1	1.2e-238	78.43	threonine dehydratase biosynthetic, chloroplastic-like isoform X2 [Cucurbita max...	[more]
KAG6604380.1	2.1e-238	77.74	Threonine dehydratase, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of CmoCh03G011860 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G10050.1	2.8e-148	51.40	L-O-methylthreonine resistant 1	[more]
AT4G11640.1	8.3e-36	30.98	serine racemase	[more]
AT3G03630.1	1.1e-06	22.53	cysteine synthase 26	[more]
AT3G61440.1	7.5e-05	24.37	cysteine synthase C1	[more]
AT3G61440.2	8.3e-04	36.54	cysteine synthase C1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036052	Tryptophan synthase beta subunit-like PLP-dependent enzyme	GENE3D	3.40.50.1100		coord: 71..350 e-value: 4.8E-97 score: 326.6
IPR036052	Tryptophan synthase beta subunit-like PLP-dependent enzyme	GENE3D	3.40.50.1100		coord: 104..195 e-value: 4.8E-97 score: 326.6
IPR036052	Tryptophan synthase beta subunit-like PLP-dependent enzyme	SUPERFAMILY	53686	Tryptophan synthase beta subunit-like PLP-dependent enzymes	coord: 63..365
IPR038110	Threonine dehydratase, ACT-like domain superfamily	GENE3D	3.40.1020.10	Biosynthetic Threonine Deaminase; Domain 3	coord: 376..550 e-value: 1.4E-42 score: 147.2
IPR001926	Pyridoxal-phosphate dependent enzyme	PFAM	PF00291	PALP	coord: 67..356 e-value: 2.8E-73 score: 246.9
IPR001721	Threonine dehydratase, ACT-like domain	PFAM	PF00585	Thr_dehydrat_C	coord: 465..548 e-value: 8.9E-19 score: 67.1
IPR001721	Threonine dehydratase, ACT-like domain	PROSITE	PS51672	ACT_LIKE	coord: 473..544 score: 10.227603
IPR005787	Threonine dehydratase, biosynthetic	TIGRFAM	TIGR01124	TIGR01124	coord: 54..544 e-value: 1.2E-168 score: 559.7
None	No IPR available	PIRSR	PIRSR604450-51	PIRSR604450-51	coord: 70..134 e-value: 0.23 score: 8.0
None	No IPR available	PIRSR	PIRSR006278-2	PIRSR006278-2	coord: 84..257 e-value: 2.5E-5 score: 21.8
None	No IPR available	PANTHER	PTHR48078	THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED	coord: 46..548
None	No IPR available	PANTHER	PTHR48078:SF11	THREONINE DEHYDRATASE, MITOCHONDRIAL	coord: 46..548
None	No IPR available	CDD	cd01562	Thr-dehyd	coord: 56..360 e-value: 1.93832E-139 score: 403.791
None	No IPR available	SUPERFAMILY	55021	ACT-like	coord: 460..549
None	No IPR available	SUPERFAMILY	55021	ACT-like	coord: 376..463
IPR000634	Serine/threonine dehydratase, pyridoxal-phosphate-binding site	PROSITE	PS00165	DEHYDRATASE_SER_THR	coord: 92..105

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh03G011860.1	CmoCh03G011860.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009097	isoleucine biosynthetic process
biological_process	GO:0006567	threonine catabolic process
biological_process	GO:0006520	cellular amino acid metabolic process
molecular_function	GO:0004794	L-threonine ammonia-lyase activity
molecular_function	GO:0030170	pyridoxal phosphate binding