CmoCh03G003860 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh03G003860
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionmitochondrial uncoupling protein 1-like
LocationCmo_Chr03: 4760507 .. 4763496 (-)
RNA-Seq ExpressionCmoCh03G003860
SyntenyCmoCh03G003860
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TAAAGAAATAACAGTGATCGTCGGAGGTGGCGGGTGACGCGGTAGACCAAGCACAAGGAAAGAGCAGCAGCCAAGACGAACAAAGCTTCGCTAACATTTCTTTCTCGTTCTGCTTTTCCCCAGATTCCCATCTTAACGACGAGACTCATTATTCTTCTTATTCCACTTTCTCTCCTTATATTTCGCTTTATTGATTTCGTCTCTGAATTTCATTTGATCTCCGTCTCGCCGGTCCAATCCTGTGGGTTTTCTCCGGTTAAGCCATGGTTGCCGATTCGAAAGGTAAATCGGACATCTCATTCGCTGGCACGTTCGCTAGTAGTGCTTTCGCTGCTTGTTTCGCTGAGGTAATGTCTTGATTTCTTACACTACCCTGTTTCTTAGGAGTTTCTTGCTGAAATTTCGTGTTGGTCTGTGTTTTGTAGCTGTCGAATTCAAATTATAACCGTGGTTTTGTGAGATAATTAGCTGGTTTTGTCTACATCTTGACCATTTTCAAATCTGGTTAACAATGTACTGGGATTGGCTCATGTGATATTTGAATAAGGTTTGATTTGAAATGGGGTAGGAGTTTGAGTGCTGTTTCGAGGGATCTTGACCATCATTTAAAGTTAATACTGAATTTTGATTATCTTTTTATATCGTGACTGTGATTTTGTAACTATTTGATTTCCTGTGTTTATGTGTGAATCCATGGAAATCAATCTACGTTGTGGTTAAGTAAGCTAGGGCCTGTTTCTTGTTCTTAGAAATTAGCTAAAATTTCAAAAGTGTTCTTAAAAGAGGGAAACATAGTGTAACAGCCCAAACCCACCCCTAGCAGAAAAAGGAGAAGGTGTTTCGTTCTCCTCCCAACCGATGTGGGATCTCACAATCCACCCCCTTCAGGACCAGCGCTCTCACTAGCACTCGTTCACTTCTCCAATCGATGTGGGACCCCCCAATTCATCCCCTTATCGGGCCAGCATCCTTGTTGACACACCTCCTCGTGTCCACCCCCTTTGGGGCTCAGCCTCCTCGCTGGCACATCGCTTGGTGTCTTTCTCTGATACCATTCATAACAGCCCGAACCCACCGTCAGCAGATATTATCCTCTTTGAGCTTTCCCTCTCCAGCTCCCCTCTCTGTTAGGGAGAGGTTTCCACACCCTTACAAAGAGTGTTTCGTTTTCCTCCCCAACTGATGTGAGATCTCACACATTGCTACAAGTAGATATTTCAAGCTTAATGATCGAAAACAGATAGCTATAACCAAAAAAACAAAACTGTTGTGTTTAAATTATGGATCTGGAATGTGTTGGCAATAATTGGCATGCATTTGATATTGATGCAGGTATGCACCATTCCTCTAGACACTGCCAAGGTTAGGCTTCAGCTACAAAAGAAAGCTGTTGCCGGTGATGCTTTACCAAAGTATAGGGGCATGCTGGGCACTGTTGCAACCATAGCCAGAGAAGAAGGTCTGGCATCCTTGTGGAAAGGCATTGTTCCCGGATTGCATCGTCAATGCGTGTATGGAGGCTTAAGGATCGGGTTGTATGAGCCTGTAAGTATTGGAAGTTCAGTCTCCATGCCAATTATTCTGTCTTTTAGCTCATATCTAACGCAAACTTGTACATCATTTACATCACAGGTTAAGACCTTTTATGTTGGCAGTGACTTTGTTGGTGATGTTCCTTTATCTAAGAAGATTCTTGCTGCACTGACAACCGGTGAGTATGCATTGGACTTTATGCTGCTTGTAACATTTGTTCTTGAATCCATAATTGCACGTCTAATGGTTGTCGAATTTGTTTCTTGTGTTAATGCCTGTATTTCTTGTTTAGGATGTCGGAAAATTTTCCATATAACTTCTTGTTTATAATTGGGAAGCAGAATAAAATGTTTCTTCCATATAACTTGTGATATTGTCCTCTTTGGGCTTTCCCTTTCGGGCTTCCCCTCAAGGTTTTTAGAACGCGTTTGCTAGGGAGAGGTTTCCGCATCCTTCTAAAGAATGCTTCGTTCTTCTCCCCAACCGATGTGGGATCTCACATAACTTCTCTTATCAACACATTTCCCTTCCCTCCTCATGTAACAGGGGCTCTAGGAATCACAATAGCAAATCCAACTGATCTTGTGAAAGTTAGACTTCAAGCGGAAGGAAAATTACCTCCCGGTGTACCTAGGCGCTATTCCGGGGCCTTGAGTGCTTATTCTACCATAGTGAGACAGGTTAGATATTCGACTTCAATATCAGGAAAAATTGTATTGCCATTTCGATTTGCTTGATATGCACGTGACATATAGGAAGGAGTTGGCGCTCTATGGACCGGCATTGGTCCAAATATTGCAAGAAATGCTATTATCAATGCCGCAGAACTAGCCAGCTATGATCAAGTGAAACAGGTATAAGCATTGGAAATTTGTGTTCTTAGAACTATTCTTGTTTCCTGTTCCCCATGCCCCGTATCGTTTGTGTCGATTCTTAGCTTATTGTATCTGTTGCAGACAATTTTGAAAATTCCAGGTTTCACAGATAATGTTATTACTCATCTTCTTGCTGGTCTTGGAGCCGGGTTTTTCGCTGTATGTATTGGCTCCCCGGTCGACGTGGTAATCTTTCTATATCCATGCTATGTCGCCTCTTGAATTCGTCACTGTTTGTATTTACTGCCTGCCCTTGTATCGAAGACCGGAATCGTTCGCTCATGATCGTATTCCTTTGTGGCAGGTGAAGTCAAGAATGATGGGGGATTCAACTTACAAAAACACACTTGATTGTTTCATCAAGACTTTAAGAAATGATGTATGTTTTCATACTCTTCCATTGTTTCCTTGATGTCTGTTTTTTTAACTTAAATACCATCTGGGACTTGCGTTTTTCAGGGACCTCTGGCATTTTACAAGGGATTTATCCCGAATTTCGGTCGGCTAGGATCATGGAACGTGATCATGTTCCTCACTCTAGAACAGGTATTTCTTGTTAGGAACTACGAACCTCCACACTAG

mRNA sequence

TAAAGAAATAACAGTGATCGTCGGAGGTGGCGGGTGACGCGGTAGACCAAGCACAAGGAAAGAGCAGCAGCCAAGACGAACAAAGCTTCGCTAACATTTCTTTCTCGTTCTGCTTTTCCCCAGATTCCCATCTTAACGACGAGACTCATTATTCTTCTTATTCCACTTTCTCTCCTTATATTTCGCTTTATTGATTTCGTCTCTGAATTTCATTTGATCTCCGTCTCGCCGGTCCAATCCTGTGGGTTTTCTCCGGTTAAGCCATGGTTGCCGATTCGAAAGGTAAATCGGACATCTCATTCGCTGGCACGTTCGCTAGTAGTGCTTTCGCTGCTTGTTTCGCTGAGCTGTCGAATTCAAATTATAACCGTGGTTTTGTATGCACCATTCCTCTAGACACTGCCAAGGTTAGGCTTCAGCTACAAAAGAAAGCTGTTGCCGGTGATGCTTTACCAAAGTATAGGGGCATGCTGGGCACTGTTGCAACCATAGCCAGAGAAGAAGGTCTGGCATCCTTGTGGAAAGGCATTGTTCCCGGATTGCATCGTCAATGCGTGTATGGAGGCTTAAGGATCGGGTTGTATGAGCCTGTTAAGACCTTTTATGTTGGCAGTGACTTTGTTGGTGATGTTCCTTTATCTAAGAAGATTCTTGCTGCACTGACAACCGGAATCACAATAGCAAATCCAACTGATCTTGTGAAAGTTAGACTTCAAGCGGAAGGAAAATTACCTCCCGGTGTACCTAGGCGCTATTCCGGGGCCTTGAGTGCTTATTCTACCATAGTGAGACAGGAAGGAGTTGGCGCTCTATGGACCGGCATTGGTCCAAATATTGCAAGAAATGCTATTATCAATGCCGCAGAACTAGCCAGCTATGATCAAGTGAAACAGACAATTTTGAAAATTCCAGGTTTCACAGATAATGTTATTACTCATCTTCTTGCTGGTCTTGGAGCCGGGTTTTTCGCTGTATGTATTGGCTCCCCGGTCGACGTGGTGAAGTCAAGAATGATGGGGGATTCAACTTACAAAAACACACTTGATTGTTTCATCAAGACTTTAAGAAATGATGGACCTCTGGCATTTTACAAGGGATTTATCCCGAATTTCGGTCGGCTAGGATCATGGAACGTGATCATGTTCCTCACTCTAGAACAGGTATTTCTTGTTAGGAACTACGAACCTCCACACTAG

Coding sequence (CDS)

ATGGTTGCCGATTCGAAAGGTAAATCGGACATCTCATTCGCTGGCACGTTCGCTAGTAGTGCTTTCGCTGCTTGTTTCGCTGAGCTGTCGAATTCAAATTATAACCGTGGTTTTGTATGCACCATTCCTCTAGACACTGCCAAGGTTAGGCTTCAGCTACAAAAGAAAGCTGTTGCCGGTGATGCTTTACCAAAGTATAGGGGCATGCTGGGCACTGTTGCAACCATAGCCAGAGAAGAAGGTCTGGCATCCTTGTGGAAAGGCATTGTTCCCGGATTGCATCGTCAATGCGTGTATGGAGGCTTAAGGATCGGGTTGTATGAGCCTGTTAAGACCTTTTATGTTGGCAGTGACTTTGTTGGTGATGTTCCTTTATCTAAGAAGATTCTTGCTGCACTGACAACCGGAATCACAATAGCAAATCCAACTGATCTTGTGAAAGTTAGACTTCAAGCGGAAGGAAAATTACCTCCCGGTGTACCTAGGCGCTATTCCGGGGCCTTGAGTGCTTATTCTACCATAGTGAGACAGGAAGGAGTTGGCGCTCTATGGACCGGCATTGGTCCAAATATTGCAAGAAATGCTATTATCAATGCCGCAGAACTAGCCAGCTATGATCAAGTGAAACAGACAATTTTGAAAATTCCAGGTTTCACAGATAATGTTATTACTCATCTTCTTGCTGGTCTTGGAGCCGGGTTTTTCGCTGTATGTATTGGCTCCCCGGTCGACGTGGTGAAGTCAAGAATGATGGGGGATTCAACTTACAAAAACACACTTGATTGTTTCATCAAGACTTTAAGAAATGATGGACCTCTGGCATTTTACAAGGGATTTATCCCGAATTTCGGTCGGCTAGGATCATGGAACGTGATCATGTTCCTCACTCTAGAACAGGTATTTCTTGTTAGGAACTACGAACCTCCACACTAG

Protein sequence

MVADSKGKSDISFAGTFASSAFAACFAELSNSNYNRGFVCTIPLDTAKVRLQLQKKAVAGDALPKYRGMLGTVATIAREEGLASLWKGIVPGLHRQCVYGGLRIGLYEPVKTFYVGSDFVGDVPLSKKILAALTTGITIANPTDLVKVRLQAEGKLPPGVPRRYSGALSAYSTIVRQEGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVITHLLAGLGAGFFAVCIGSPVDVVKSRMMGDSTYKNTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQVFLVRNYEPPH
Homology
BLAST of CmoCh03G003860 vs. ExPASy Swiss-Prot
Match: O81845 (Mitochondrial uncoupling protein 1 OS=Arabidopsis thaliana OX=3702 GN=PUMP1 PE=1 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 4.2e-135
Identity = 242/298 (81.21%), Postives = 262/298 (87.92%), Query Frame = 0

Query: 7   GKSDISFAGTFASSAFAACFAELSNSNYNRGFVCTIPLDTAKVRLQLQKKAVAGD-ALPK 66
           GKSD+S   TFA SAFAAC  E          VCTIPLDTAKVRLQLQK A+AGD  LPK
Sbjct: 5   GKSDLSLPKTFACSAFAACVGE----------VCTIPLDTAKVRLQLQKSALAGDVTLPK 64

Query: 67  YRGMLGTVATIAREEGLASLWKGIVPGLHRQCVYGGLRIGLYEPVKTFYVGSDFVGDVPL 126
           YRG+LGTV TIAREEGL SLWKG+VPGLHRQC++GGLRIG+YEPVK  YVG DFVGDVPL
Sbjct: 65  YRGLLGTVGTIAREEGLRSLWKGVVPGLHRQCLFGGLRIGMYEPVKNLYVGKDFVGDVPL 124

Query: 127 SKKILAALTT---GITIANPTDLVKVRLQAEGKLPPGVPRRYSGALSAYSTIVRQEGVGA 186
           SKKILA LTT   GI +ANPTDLVKVRLQAEGKL  G PRRYSGAL+AYSTIVRQEGV A
Sbjct: 125 SKKILAGLTTGALGIMVANPTDLVKVRLQAEGKLAAGAPRRYSGALNAYSTIVRQEGVRA 184

Query: 187 LWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVITHLLAGLGAGFFAVCIGSP 246
           LWTG+GPN+ARNAIINAAELASYDQVK+TILKIPGFTDNV+TH+L+GLGAGFFAVCIGSP
Sbjct: 185 LWTGLGPNVARNAIINAAELASYDQVKETILKIPGFTDNVVTHILSGLGAGFFAVCIGSP 244

Query: 247 VDVVKSRMMGDS-TYKNTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQ 300
           VDVVKSRMMGDS  YK T+DCF+KTL++DGP+AFYKGFIPNFGRLGSWNVIMFLTLEQ
Sbjct: 245 VDVVKSRMMGDSGAYKGTIDCFVKTLKSDGPMAFYKGFIPNFGRLGSWNVIMFLTLEQ 292

BLAST of CmoCh03G003860 vs. ExPASy Swiss-Prot
Match: Q9ZWG1 (Mitochondrial uncoupling protein 2 OS=Arabidopsis thaliana OX=3702 GN=PUMP2 PE=2 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 1.6e-126
Identity = 223/304 (73.36%), Postives = 258/304 (84.87%), Query Frame = 0

Query: 2   VADSKGKSDISFAGTFASSAFAACFAELSNSNYNRGFVCTIPLDTAKVRLQLQKKAVAGD 61
           +AD K + +ISF  TF  SAFAACFAEL          CTIPLDTAKVRLQLQ+K   GD
Sbjct: 1   MADFKPRIEISFLETFICSAFAACFAEL----------CTIPLDTAKVRLQLQRKIPTGD 60

Query: 62  A--LPKYRGMLGTVATIAREEGLASLWKGIVPGLHRQCVYGGLRIGLYEPVKTFYVGSDF 121
              LPKYRG +GT+ATIAREEG++ LWKG++ GLHRQC+YGGLRIGLYEPVKT  VGSDF
Sbjct: 61  GENLPKYRGSIGTLATIAREEGISGLWKGVIAGLHRQCIYGGLRIGLYEPVKTLLVGSDF 120

Query: 122 VGDVPLSKKILAALTTG---ITIANPTDLVKVRLQAEGKLPPGVPRRYSGALSAYSTIVR 181
           +GD+PL +KILAAL TG   I +ANPTDLVKVRLQ+EGKLP GVPRRY+GA+ AY TIV+
Sbjct: 121 IGDIPLYQKILAALLTGAIAIIVANPTDLVKVRLQSEGKLPAGVPRRYAGAVDAYFTIVK 180

Query: 182 QEGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVITHLLAGLGAGFFA 241
            EGV ALWTG+GPNIARNAI+NAAELASYDQ+K+TI+KIP F D+V+THLLAGL AGFFA
Sbjct: 181 LEGVSALWTGLGPNIARNAIVNAAELASYDQIKETIMKIPFFRDSVLTHLLAGLAAGFFA 240

Query: 242 VCIGSPVDVVKSRMMGDSTYKNTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLT 301
           VCIGSP+DVVKSRMMGDSTY+NT+DCFIKT++ +G +AFYKGF+PNF RLG+WN IMFLT
Sbjct: 241 VCIGSPIDVVKSRMMGDSTYRNTVDCFIKTMKTEGIMAFYKGFLPNFTRLGTWNAIMFLT 294

BLAST of CmoCh03G003860 vs. ExPASy Swiss-Prot
Match: O97649 (Mitochondrial uncoupling protein 3 OS=Sus scrofa OX=9823 GN=UCP3 PE=2 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 1.7e-67
Identity = 140/285 (49.12%), Postives = 181/285 (63.51%), Query Frame = 0

Query: 23  AACFAELSNSNYNRGFVCTIPLDTAKVRLQLQKKAVAGDALPKYRGMLGTVATIAREEGL 82
           AACFA+L           T PLDTAKVRLQ+Q +  A  +  +YRG+LGT+ T+ R EG 
Sbjct: 23  AACFADL----------LTFPLDTAKVRLQIQGENQAARS-AQYRGVLGTILTMVRNEGP 82

Query: 83  ASLWKGIVPGLHRQCVYGGLRIGLYEPVKTFYV--GSDFVGDVPLSKKILAALTTG---I 142
            S + G+V GL RQ  +  +RIGLY+ VK  Y   GSD      ++ +ILA  TTG   +
Sbjct: 83  RSPYNGLVAGLQRQMSFASIRIGLYDSVKQLYTPKGSDHSS---ITTRILAGCTTGAMAV 142

Query: 143 TIANPTDLVKVRLQAEGKLPPGVPRRYSGALSAYSTIVRQEGVGALWTGIGPNIARNAII 202
           T A PTD+VKVR QA     P   R+YSG + AY TI R+EGV  LW GI PNI RNAI+
Sbjct: 143 TCAQPTDVVKVRFQASIHAGPRSNRKYSGTMDAYRTIAREEGVRGLWKGILPNITRNAIV 202

Query: 203 NAAELASYDQVKQTILKIPGFTDNVITHLLAGLGAGFFAVCIGSPVDVVKSRMMGD--ST 262
           N AE+ +YD +K+ +L     TDN+  H ++  GAGF A  + SPVDVVK+R M      
Sbjct: 203 NCAEMVTYDVIKEKVLDYHLLTDNLPCHFVSAFGAGFCATVVASPVDVVKTRYMNSPPGQ 262

Query: 263 YKNTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQV 301
           Y+N LDC +K +  +GP AFYKGF P+F RLGSWNV+MF++ EQ+
Sbjct: 263 YQNPLDCMLKMVTQEGPTAFYKGFTPSFLRLGSWNVVMFVSYEQL 293

BLAST of CmoCh03G003860 vs. ExPASy Swiss-Prot
Match: P56501 (Mitochondrial uncoupling protein 3 OS=Mus musculus OX=10090 GN=Ucp3 PE=1 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 2.2e-67
Identity = 139/291 (47.77%), Postives = 186/291 (63.92%), Query Frame = 0

Query: 17  FASSAFAACFAELSNSNYNRGFVCTIPLDTAKVRLQLQKKAVAGDALPKYRGMLGTVATI 76
           F  +  AACFA+L           T PLDTAKVRLQ+Q +     ++ +YRG+LGT+ T+
Sbjct: 17  FLGAGTAACFADL----------LTFPLDTAKVRLQIQGENPGAQSV-QYRGVLGTILTM 76

Query: 77  AREEGLASLWKGIVPGLHRQCVYGGLRIGLYEPVKTFYV--GSDFVGDVPLSKKILAALT 136
            R EG  S + G+V GLHRQ  +  +RIGLY+ VK FY   G+D      ++ +ILA  T
Sbjct: 77  VRTEGPRSPYSGLVAGLHRQMSFASIRIGLYDSVKQFYTPKGADHSS---VAIRILAGCT 136

Query: 137 TG---ITIANPTDLVKVRLQAEGKLPPGVPRRYSGALSAYSTIVRQEGVGALWTGIGPNI 196
           TG   +T A PTD+VKVR QA  +L  G  R+Y G + AY TI R+EGV  LW G  PNI
Sbjct: 137 TGAMAVTCAQPTDVVKVRFQAMIRLGTGGERKYRGTMDAYRTIAREEGVRGLWKGTWPNI 196

Query: 197 ARNAIINAAELASYDQVKQTILKIPGFTDNVITHLLAGLGAGFFAVCIGSPVDVVKSRMM 256
            RNAI+N AE+ +YD +K+ +L+   FTDN   H ++  GAGF A  + SPVDVVK+R M
Sbjct: 197 TRNAIVNCAEMVTYDIIKEKLLESHLFTDNFPCHFVSAFGAGFCATVVASPVDVVKTRYM 256

Query: 257 GD--STYKNTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQV 301
                 Y++ L C +K +  +GP AFYKGF+P+F RLG+WNV+MF+T EQ+
Sbjct: 257 NAPLGRYRSPLHCMLKMVAQEGPTAFYKGFVPSFLRLGAWNVMMFVTYEQL 293

BLAST of CmoCh03G003860 vs. ExPASy Swiss-Prot
Match: Q9N2I9 (Mitochondrial uncoupling protein 3 OS=Canis lupus familiaris OX=9615 GN=UCP3 PE=2 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 4.9e-67
Identity = 142/293 (48.46%), Postives = 183/293 (62.46%), Query Frame = 0

Query: 17  FASSAFAACFAELSNSNYNRGFVCTIPLDTAKVRLQLQKKAVAGDALPK--YRGMLGTVA 76
           F  +  AACFA+L           T PLDTAKVRLQ+Q +  A  A  +  YRG+LGT+ 
Sbjct: 17  FLGAGTAACFADL----------LTFPLDTAKVRLQIQGENQATQAARRIQYRGVLGTIL 76

Query: 77  TIAREEGLASLWKGIVPGLHRQCVYGGLRIGLYEPVKTFYV--GSDFVGDVPLSKKILAA 136
           T+ R EG  S + G+V GL RQ  +  +RIGLY+ VK FY   GSD      ++ +ILA 
Sbjct: 77  TMVRTEGPRSPYNGLVAGLQRQMSFASIRIGLYDSVKQFYTPKGSDHSS---ITTRILAG 136

Query: 137 LTTG---ITIANPTDLVKVRLQAEGKLPPGVPRRYSGALSAYSTIVRQEGVGALWTGIGP 196
            TTG   ++ A PTD+VKVR QA   L  G  R+YSG + AY TI R+EGV  LW G  P
Sbjct: 137 CTTGAMAVSCAQPTDVVKVRFQASIHLGAGSNRKYSGTMDAYRTIAREEGVRGLWKGTLP 196

Query: 197 NIARNAIINAAELASYDQVKQTILKIPGFTDNVITHLLAGLGAGFFAVCIGSPVDVVKSR 256
           NI RNAI+N AE+ +YD +K+ +L     TDN   HL++  GAGF A  + SPVDVVK+R
Sbjct: 197 NITRNAIVNCAEMVTYDIIKEKLLDYHLLTDNFPCHLISAFGAGFCATVVASPVDVVKTR 256

Query: 257 MMGD--STYKNTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQV 301
            M      Y + LDC +K +  +GP AFYKGF P+F RLG+WNV+MF+T EQ+
Sbjct: 257 YMNSPPGQYCSPLDCMLKMVTQEGPTAFYKGFTPSFLRLGTWNVVMFVTYEQL 296

BLAST of CmoCh03G003860 vs. ExPASy TrEMBL
Match: A0A6J1IM65 (mitochondrial uncoupling protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111478283 PE=3 SV=1)

HSP 1 Score: 565.5 bits (1456), Expect = 1.4e-157
Identity = 289/302 (95.70%), Postives = 289/302 (95.70%), Query Frame = 0

Query: 1   MVADSKGKSDISFAGTFASSAFAACFAELSNSNYNRGFVCTIPLDTAKVRLQLQKKAVAG 60
           MVADSKGKSDISFAGTFASSAFAACFAE          VCTIPLDTAKVRLQLQKKAVAG
Sbjct: 1   MVADSKGKSDISFAGTFASSAFAACFAE----------VCTIPLDTAKVRLQLQKKAVAG 60

Query: 61  DALPKYRGMLGTVATIAREEGLASLWKGIVPGLHRQCVYGGLRIGLYEPVKTFYVGSDFV 120
           DALPKYRGMLGTVATIAREEGLASLWKGIVPGLHRQCVYGGLRIGLYEPVKTFYVGSDFV
Sbjct: 61  DALPKYRGMLGTVATIAREEGLASLWKGIVPGLHRQCVYGGLRIGLYEPVKTFYVGSDFV 120

Query: 121 GDVPLSKKILAALTT---GITIANPTDLVKVRLQAEGKLPPGVPRRYSGALSAYSTIVRQ 180
           GDVPLSKKILAALTT   GITIANPTDLVKVRLQAEGKLPPGVPRRYSGALSAYSTIVRQ
Sbjct: 121 GDVPLSKKILAALTTGALGITIANPTDLVKVRLQAEGKLPPGVPRRYSGALSAYSTIVRQ 180

Query: 181 EGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVITHLLAGLGAGFFAV 240
           EGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVITHLLAGLGAGFFAV
Sbjct: 181 EGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVITHLLAGLGAGFFAV 240

Query: 241 CIGSPVDVVKSRMMGDSTYKNTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTL 300
           CIGSPVDVVKSRMMGDSTYKNTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTL
Sbjct: 241 CIGSPVDVVKSRMMGDSTYKNTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTL 292

BLAST of CmoCh03G003860 vs. ExPASy TrEMBL
Match: A0A6J1GFM7 (mitochondrial uncoupling protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC111453723 PE=3 SV=1)

HSP 1 Score: 565.5 bits (1456), Expect = 1.4e-157
Identity = 289/302 (95.70%), Postives = 289/302 (95.70%), Query Frame = 0

Query: 1   MVADSKGKSDISFAGTFASSAFAACFAELSNSNYNRGFVCTIPLDTAKVRLQLQKKAVAG 60
           MVADSKGKSDISFAGTFASSAFAACFAE          VCTIPLDTAKVRLQLQKKAVAG
Sbjct: 1   MVADSKGKSDISFAGTFASSAFAACFAE----------VCTIPLDTAKVRLQLQKKAVAG 60

Query: 61  DALPKYRGMLGTVATIAREEGLASLWKGIVPGLHRQCVYGGLRIGLYEPVKTFYVGSDFV 120
           DALPKYRGMLGTVATIAREEGLASLWKGIVPGLHRQCVYGGLRIGLYEPVKTFYVGSDFV
Sbjct: 61  DALPKYRGMLGTVATIAREEGLASLWKGIVPGLHRQCVYGGLRIGLYEPVKTFYVGSDFV 120

Query: 121 GDVPLSKKILAALTT---GITIANPTDLVKVRLQAEGKLPPGVPRRYSGALSAYSTIVRQ 180
           GDVPLSKKILAALTT   GITIANPTDLVKVRLQAEGKLPPGVPRRYSGALSAYSTIVRQ
Sbjct: 121 GDVPLSKKILAALTTGALGITIANPTDLVKVRLQAEGKLPPGVPRRYSGALSAYSTIVRQ 180

Query: 181 EGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVITHLLAGLGAGFFAV 240
           EGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVITHLLAGLGAGFFAV
Sbjct: 181 EGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVITHLLAGLGAGFFAV 240

Query: 241 CIGSPVDVVKSRMMGDSTYKNTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTL 300
           CIGSPVDVVKSRMMGDSTYKNTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTL
Sbjct: 241 CIGSPVDVVKSRMMGDSTYKNTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTL 292

BLAST of CmoCh03G003860 vs. ExPASy TrEMBL
Match: A0A5A7TT48 (Mitochondrial uncoupling protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold78209G00510 PE=3 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 2.5e-154
Identity = 283/311 (91.00%), Postives = 290/311 (93.25%), Query Frame = 0

Query: 1   MVADSKGKSDISFAGTFASSAFAACFAELSNSNYNRGFVCTIPLDTAKVRLQLQKKAVAG 60
           MVADSKGKSDISFAGTFASSAFAACFAE          +CTIPLDTAKVRLQLQKKAVAG
Sbjct: 1   MVADSKGKSDISFAGTFASSAFAACFAE----------ICTIPLDTAKVRLQLQKKAVAG 60

Query: 61  DALPKYRGMLGTVATIAREEGLASLWKGIVPGLHRQCVYGGLRIGLYEPVKTFYVGSDFV 120
           D LPKYRGMLGTVATIAREEGLASLWKGIVPGLHRQC++GGLRIG+YEPVK FYVGSDFV
Sbjct: 61  DVLPKYRGMLGTVATIAREEGLASLWKGIVPGLHRQCLFGGLRIGMYEPVKNFYVGSDFV 120

Query: 121 GDVPLSKKILAALTT---GITIANPTDLVKVRLQAEGKLPPGVPRRYSGALSAYSTIVRQ 180
           GDVPLSKKILAALTT   GITIANPTDLVKVRLQAEGKLPPG PRRYSGAL+AYSTIVRQ
Sbjct: 121 GDVPLSKKILAALTTGALGITIANPTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQ 180

Query: 181 EGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVITHLLAGLGAGFFAV 240
           EGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNV+THLLAGLGAGFFAV
Sbjct: 181 EGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAV 240

Query: 241 CIGSPVDVVKSRMMGDSTYKNTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTL 300
           CIGSPVDVVKSRMMGDSTYK+TLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTL
Sbjct: 241 CIGSPVDVVKSRMMGDSTYKSTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTL 300

Query: 301 EQV-FLVRNYE 308
           EQ    VRN E
Sbjct: 301 EQAKKFVRNIE 301

BLAST of CmoCh03G003860 vs. ExPASy TrEMBL
Match: A0A1S3BLL8 (mitochondrial uncoupling protein 1 OS=Cucumis melo OX=3656 GN=LOC103490980 PE=3 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 2.5e-154
Identity = 283/311 (91.00%), Postives = 290/311 (93.25%), Query Frame = 0

Query: 1   MVADSKGKSDISFAGTFASSAFAACFAELSNSNYNRGFVCTIPLDTAKVRLQLQKKAVAG 60
           MVADSKGKSDISFAGTFASSAFAACFAE          +CTIPLDTAKVRLQLQKKAVAG
Sbjct: 1   MVADSKGKSDISFAGTFASSAFAACFAE----------ICTIPLDTAKVRLQLQKKAVAG 60

Query: 61  DALPKYRGMLGTVATIAREEGLASLWKGIVPGLHRQCVYGGLRIGLYEPVKTFYVGSDFV 120
           D LPKYRGMLGTVATIAREEGLASLWKGIVPGLHRQC++GGLRIG+YEPVK FYVGSDFV
Sbjct: 61  DVLPKYRGMLGTVATIAREEGLASLWKGIVPGLHRQCLFGGLRIGMYEPVKNFYVGSDFV 120

Query: 121 GDVPLSKKILAALTT---GITIANPTDLVKVRLQAEGKLPPGVPRRYSGALSAYSTIVRQ 180
           GDVPLSKKILAALTT   GITIANPTDLVKVRLQAEGKLPPG PRRYSGAL+AYSTIVRQ
Sbjct: 121 GDVPLSKKILAALTTGALGITIANPTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQ 180

Query: 181 EGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVITHLLAGLGAGFFAV 240
           EGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNV+THLLAGLGAGFFAV
Sbjct: 181 EGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAV 240

Query: 241 CIGSPVDVVKSRMMGDSTYKNTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTL 300
           CIGSPVDVVKSRMMGDSTYK+TLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTL
Sbjct: 241 CIGSPVDVVKSRMMGDSTYKSTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTL 300

Query: 301 EQV-FLVRNYE 308
           EQ    VRN E
Sbjct: 301 EQAKKFVRNIE 301

BLAST of CmoCh03G003860 vs. ExPASy TrEMBL
Match: A0A6J1HUA8 (mitochondrial uncoupling protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111466242 PE=3 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 4.7e-153
Identity = 276/302 (91.39%), Postives = 286/302 (94.70%), Query Frame = 0

Query: 1   MVADSKGKSDISFAGTFASSAFAACFAELSNSNYNRGFVCTIPLDTAKVRLQLQKKAVAG 60
           MVADSKGKSDISFAGTFASSAFAACFAE          +CTIPLDTAKVRLQLQKKAV G
Sbjct: 1   MVADSKGKSDISFAGTFASSAFAACFAE----------ICTIPLDTAKVRLQLQKKAVVG 60

Query: 61  DALPKYRGMLGTVATIAREEGLASLWKGIVPGLHRQCVYGGLRIGLYEPVKTFYVGSDFV 120
           D LPKYRGMLGTVATIAREEG+ASLWKGIVPGLHRQCV+GGLRIGLYEPVK FYVGSDFV
Sbjct: 61  DVLPKYRGMLGTVATIAREEGMASLWKGIVPGLHRQCVFGGLRIGLYEPVKNFYVGSDFV 120

Query: 121 GDVPLSKKILAALTT---GITIANPTDLVKVRLQAEGKLPPGVPRRYSGALSAYSTIVRQ 180
           GDVPL+KKILAALTT   GIT+ANPTDLVKVRLQAEGKLPPGVPRRYSGAL+AYSTIVRQ
Sbjct: 121 GDVPLTKKILAALTTGAIGITVANPTDLVKVRLQAEGKLPPGVPRRYSGALNAYSTIVRQ 180

Query: 181 EGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVITHLLAGLGAGFFAV 240
           EGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNV+THLL+GLGAGFFAV
Sbjct: 181 EGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVLTHLLSGLGAGFFAV 240

Query: 241 CIGSPVDVVKSRMMGDSTYKNTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTL 300
           CIGSPVDVVKSRMMGDSTYK+T+DCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTL
Sbjct: 241 CIGSPVDVVKSRMMGDSTYKSTIDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTL 292

BLAST of CmoCh03G003860 vs. NCBI nr
Match: XP_022950708.1 (mitochondrial uncoupling protein 1-like [Cucurbita moschata] >XP_022978241.1 mitochondrial uncoupling protein 1-like [Cucurbita maxima] >KAG6603605.1 Mitochondrial uncoupling protein 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 565.5 bits (1456), Expect = 2.9e-157
Identity = 289/302 (95.70%), Postives = 289/302 (95.70%), Query Frame = 0

Query: 1   MVADSKGKSDISFAGTFASSAFAACFAELSNSNYNRGFVCTIPLDTAKVRLQLQKKAVAG 60
           MVADSKGKSDISFAGTFASSAFAACFAE          VCTIPLDTAKVRLQLQKKAVAG
Sbjct: 1   MVADSKGKSDISFAGTFASSAFAACFAE----------VCTIPLDTAKVRLQLQKKAVAG 60

Query: 61  DALPKYRGMLGTVATIAREEGLASLWKGIVPGLHRQCVYGGLRIGLYEPVKTFYVGSDFV 120
           DALPKYRGMLGTVATIAREEGLASLWKGIVPGLHRQCVYGGLRIGLYEPVKTFYVGSDFV
Sbjct: 61  DALPKYRGMLGTVATIAREEGLASLWKGIVPGLHRQCVYGGLRIGLYEPVKTFYVGSDFV 120

Query: 121 GDVPLSKKILAALTT---GITIANPTDLVKVRLQAEGKLPPGVPRRYSGALSAYSTIVRQ 180
           GDVPLSKKILAALTT   GITIANPTDLVKVRLQAEGKLPPGVPRRYSGALSAYSTIVRQ
Sbjct: 121 GDVPLSKKILAALTTGALGITIANPTDLVKVRLQAEGKLPPGVPRRYSGALSAYSTIVRQ 180

Query: 181 EGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVITHLLAGLGAGFFAV 240
           EGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVITHLLAGLGAGFFAV
Sbjct: 181 EGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVITHLLAGLGAGFFAV 240

Query: 241 CIGSPVDVVKSRMMGDSTYKNTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTL 300
           CIGSPVDVVKSRMMGDSTYKNTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTL
Sbjct: 241 CIGSPVDVVKSRMMGDSTYKNTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTL 292

BLAST of CmoCh03G003860 vs. NCBI nr
Match: XP_023543070.1 (mitochondrial uncoupling protein 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 563.9 bits (1452), Expect = 8.4e-157
Identity = 288/302 (95.36%), Postives = 288/302 (95.36%), Query Frame = 0

Query: 1   MVADSKGKSDISFAGTFASSAFAACFAELSNSNYNRGFVCTIPLDTAKVRLQLQKKAVAG 60
           MVADSKGKSDISFAGTFASSAFAACFAE          VCTIPLDTAKVRLQLQKKAVAG
Sbjct: 1   MVADSKGKSDISFAGTFASSAFAACFAE----------VCTIPLDTAKVRLQLQKKAVAG 60

Query: 61  DALPKYRGMLGTVATIAREEGLASLWKGIVPGLHRQCVYGGLRIGLYEPVKTFYVGSDFV 120
           DALPKYRGMLGTVATIAREEGLASLWKGIVPGLHRQCVYGGLRIGLYEPVKTFYVGSDFV
Sbjct: 61  DALPKYRGMLGTVATIAREEGLASLWKGIVPGLHRQCVYGGLRIGLYEPVKTFYVGSDFV 120

Query: 121 GDVPLSKKILAALTT---GITIANPTDLVKVRLQAEGKLPPGVPRRYSGALSAYSTIVRQ 180
           GDVPLSKKILAALTT   GITIANPTDLVKVRLQAEGKLPPGVPRRYSGALSAYSTIVRQ
Sbjct: 121 GDVPLSKKILAALTTGALGITIANPTDLVKVRLQAEGKLPPGVPRRYSGALSAYSTIVRQ 180

Query: 181 EGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVITHLLAGLGAGFFAV 240
           EGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVITHL AGLGAGFFAV
Sbjct: 181 EGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVITHLFAGLGAGFFAV 240

Query: 241 CIGSPVDVVKSRMMGDSTYKNTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTL 300
           CIGSPVDVVKSRMMGDSTYKNTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTL
Sbjct: 241 CIGSPVDVVKSRMMGDSTYKNTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTL 292

BLAST of CmoCh03G003860 vs. NCBI nr
Match: XP_038880991.1 (mitochondrial uncoupling protein 1 [Benincasa hispida])

HSP 1 Score: 555.1 bits (1429), Expect = 3.9e-154
Identity = 283/311 (91.00%), Postives = 290/311 (93.25%), Query Frame = 0

Query: 1   MVADSKGKSDISFAGTFASSAFAACFAELSNSNYNRGFVCTIPLDTAKVRLQLQKKAVAG 60
           MVADSKGKSDISFAGTFASSAFAACFAE          VCTIPLDTAKVRLQLQKKAVAG
Sbjct: 1   MVADSKGKSDISFAGTFASSAFAACFAE----------VCTIPLDTAKVRLQLQKKAVAG 60

Query: 61  DALPKYRGMLGTVATIAREEGLASLWKGIVPGLHRQCVYGGLRIGLYEPVKTFYVGSDFV 120
           D LPKYRGMLGTVATIAREEGLASLWKGIVPGLHRQCV+GGLRIG+YEPVK FYVGSDFV
Sbjct: 61  DVLPKYRGMLGTVATIAREEGLASLWKGIVPGLHRQCVFGGLRIGMYEPVKNFYVGSDFV 120

Query: 121 GDVPLSKKILAALTT---GITIANPTDLVKVRLQAEGKLPPGVPRRYSGALSAYSTIVRQ 180
           GDVPLSKKILAALTT   GIT+ANPTDLVKVRLQAEGKLPPGVPRRYSGAL+AYSTIVRQ
Sbjct: 121 GDVPLSKKILAALTTGALGITVANPTDLVKVRLQAEGKLPPGVPRRYSGALNAYSTIVRQ 180

Query: 181 EGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVITHLLAGLGAGFFAV 240
           EG+GALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNV+THLLAGLGAGFFAV
Sbjct: 181 EGIGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAV 240

Query: 241 CIGSPVDVVKSRMMGDSTYKNTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTL 300
           CIGSPVDVVKSRMMGDS YK+TLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTL
Sbjct: 241 CIGSPVDVVKSRMMGDSAYKSTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTL 300

Query: 301 EQV-FLVRNYE 308
           EQ    VRN E
Sbjct: 301 EQAKKFVRNIE 301

BLAST of CmoCh03G003860 vs. NCBI nr
Match: XP_008448974.1 (PREDICTED: mitochondrial uncoupling protein 1 [Cucumis melo] >KAA0045067.1 mitochondrial uncoupling protein 1 [Cucumis melo var. makuwa] >TYJ96260.1 mitochondrial uncoupling protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 554.7 bits (1428), Expect = 5.1e-154
Identity = 283/311 (91.00%), Postives = 290/311 (93.25%), Query Frame = 0

Query: 1   MVADSKGKSDISFAGTFASSAFAACFAELSNSNYNRGFVCTIPLDTAKVRLQLQKKAVAG 60
           MVADSKGKSDISFAGTFASSAFAACFAE          +CTIPLDTAKVRLQLQKKAVAG
Sbjct: 1   MVADSKGKSDISFAGTFASSAFAACFAE----------ICTIPLDTAKVRLQLQKKAVAG 60

Query: 61  DALPKYRGMLGTVATIAREEGLASLWKGIVPGLHRQCVYGGLRIGLYEPVKTFYVGSDFV 120
           D LPKYRGMLGTVATIAREEGLASLWKGIVPGLHRQC++GGLRIG+YEPVK FYVGSDFV
Sbjct: 61  DVLPKYRGMLGTVATIAREEGLASLWKGIVPGLHRQCLFGGLRIGMYEPVKNFYVGSDFV 120

Query: 121 GDVPLSKKILAALTT---GITIANPTDLVKVRLQAEGKLPPGVPRRYSGALSAYSTIVRQ 180
           GDVPLSKKILAALTT   GITIANPTDLVKVRLQAEGKLPPG PRRYSGAL+AYSTIVRQ
Sbjct: 121 GDVPLSKKILAALTTGALGITIANPTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQ 180

Query: 181 EGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVITHLLAGLGAGFFAV 240
           EGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNV+THLLAGLGAGFFAV
Sbjct: 181 EGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAV 240

Query: 241 CIGSPVDVVKSRMMGDSTYKNTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTL 300
           CIGSPVDVVKSRMMGDSTYK+TLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTL
Sbjct: 241 CIGSPVDVVKSRMMGDSTYKSTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTL 300

Query: 301 EQV-FLVRNYE 308
           EQ    VRN E
Sbjct: 301 EQAKKFVRNIE 301

BLAST of CmoCh03G003860 vs. NCBI nr
Match: XP_022966614.1 (mitochondrial uncoupling protein 1-like [Cucurbita maxima])

HSP 1 Score: 550.4 bits (1417), Expect = 9.7e-153
Identity = 276/302 (91.39%), Postives = 286/302 (94.70%), Query Frame = 0

Query: 1   MVADSKGKSDISFAGTFASSAFAACFAELSNSNYNRGFVCTIPLDTAKVRLQLQKKAVAG 60
           MVADSKGKSDISFAGTFASSAFAACFAE          +CTIPLDTAKVRLQLQKKAV G
Sbjct: 1   MVADSKGKSDISFAGTFASSAFAACFAE----------ICTIPLDTAKVRLQLQKKAVVG 60

Query: 61  DALPKYRGMLGTVATIAREEGLASLWKGIVPGLHRQCVYGGLRIGLYEPVKTFYVGSDFV 120
           D LPKYRGMLGTVATIAREEG+ASLWKGIVPGLHRQCV+GGLRIGLYEPVK FYVGSDFV
Sbjct: 61  DVLPKYRGMLGTVATIAREEGMASLWKGIVPGLHRQCVFGGLRIGLYEPVKNFYVGSDFV 120

Query: 121 GDVPLSKKILAALTT---GITIANPTDLVKVRLQAEGKLPPGVPRRYSGALSAYSTIVRQ 180
           GDVPL+KKILAALTT   GIT+ANPTDLVKVRLQAEGKLPPGVPRRYSGAL+AYSTIVRQ
Sbjct: 121 GDVPLTKKILAALTTGAIGITVANPTDLVKVRLQAEGKLPPGVPRRYSGALNAYSTIVRQ 180

Query: 181 EGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVITHLLAGLGAGFFAV 240
           EGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNV+THLL+GLGAGFFAV
Sbjct: 181 EGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVLTHLLSGLGAGFFAV 240

Query: 241 CIGSPVDVVKSRMMGDSTYKNTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTL 300
           CIGSPVDVVKSRMMGDSTYK+T+DCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTL
Sbjct: 241 CIGSPVDVVKSRMMGDSTYKSTIDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTL 292

BLAST of CmoCh03G003860 vs. TAIR 10
Match: AT3G54110.1 (plant uncoupling mitochondrial protein 1 )

HSP 1 Score: 482.3 bits (1240), Expect = 3.0e-136
Identity = 242/298 (81.21%), Postives = 262/298 (87.92%), Query Frame = 0

Query: 7   GKSDISFAGTFASSAFAACFAELSNSNYNRGFVCTIPLDTAKVRLQLQKKAVAGD-ALPK 66
           GKSD+S   TFA SAFAAC  E          VCTIPLDTAKVRLQLQK A+AGD  LPK
Sbjct: 5   GKSDLSLPKTFACSAFAACVGE----------VCTIPLDTAKVRLQLQKSALAGDVTLPK 64

Query: 67  YRGMLGTVATIAREEGLASLWKGIVPGLHRQCVYGGLRIGLYEPVKTFYVGSDFVGDVPL 126
           YRG+LGTV TIAREEGL SLWKG+VPGLHRQC++GGLRIG+YEPVK  YVG DFVGDVPL
Sbjct: 65  YRGLLGTVGTIAREEGLRSLWKGVVPGLHRQCLFGGLRIGMYEPVKNLYVGKDFVGDVPL 124

Query: 127 SKKILAALTT---GITIANPTDLVKVRLQAEGKLPPGVPRRYSGALSAYSTIVRQEGVGA 186
           SKKILA LTT   GI +ANPTDLVKVRLQAEGKL  G PRRYSGAL+AYSTIVRQEGV A
Sbjct: 125 SKKILAGLTTGALGIMVANPTDLVKVRLQAEGKLAAGAPRRYSGALNAYSTIVRQEGVRA 184

Query: 187 LWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVITHLLAGLGAGFFAVCIGSP 246
           LWTG+GPN+ARNAIINAAELASYDQVK+TILKIPGFTDNV+TH+L+GLGAGFFAVCIGSP
Sbjct: 185 LWTGLGPNVARNAIINAAELASYDQVKETILKIPGFTDNVVTHILSGLGAGFFAVCIGSP 244

Query: 247 VDVVKSRMMGDS-TYKNTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQ 300
           VDVVKSRMMGDS  YK T+DCF+KTL++DGP+AFYKGFIPNFGRLGSWNVIMFLTLEQ
Sbjct: 245 VDVVKSRMMGDSGAYKGTIDCFVKTLKSDGPMAFYKGFIPNFGRLGSWNVIMFLTLEQ 292

BLAST of CmoCh03G003860 vs. TAIR 10
Match: AT5G58970.1 (uncoupling protein 2 )

HSP 1 Score: 453.8 bits (1166), Expect = 1.1e-127
Identity = 223/304 (73.36%), Postives = 258/304 (84.87%), Query Frame = 0

Query: 2   VADSKGKSDISFAGTFASSAFAACFAELSNSNYNRGFVCTIPLDTAKVRLQLQKKAVAGD 61
           +AD K + +ISF  TF  SAFAACFAEL          CTIPLDTAKVRLQLQ+K   GD
Sbjct: 1   MADFKPRIEISFLETFICSAFAACFAEL----------CTIPLDTAKVRLQLQRKIPTGD 60

Query: 62  A--LPKYRGMLGTVATIAREEGLASLWKGIVPGLHRQCVYGGLRIGLYEPVKTFYVGSDF 121
              LPKYRG +GT+ATIAREEG++ LWKG++ GLHRQC+YGGLRIGLYEPVKT  VGSDF
Sbjct: 61  GENLPKYRGSIGTLATIAREEGISGLWKGVIAGLHRQCIYGGLRIGLYEPVKTLLVGSDF 120

Query: 122 VGDVPLSKKILAALTTG---ITIANPTDLVKVRLQAEGKLPPGVPRRYSGALSAYSTIVR 181
           +GD+PL +KILAAL TG   I +ANPTDLVKVRLQ+EGKLP GVPRRY+GA+ AY TIV+
Sbjct: 121 IGDIPLYQKILAALLTGAIAIIVANPTDLVKVRLQSEGKLPAGVPRRYAGAVDAYFTIVK 180

Query: 182 QEGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVITHLLAGLGAGFFA 241
            EGV ALWTG+GPNIARNAI+NAAELASYDQ+K+TI+KIP F D+V+THLLAGL AGFFA
Sbjct: 181 LEGVSALWTGLGPNIARNAIVNAAELASYDQIKETIMKIPFFRDSVLTHLLAGLAAGFFA 240

Query: 242 VCIGSPVDVVKSRMMGDSTYKNTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLT 301
           VCIGSP+DVVKSRMMGDSTY+NT+DCFIKT++ +G +AFYKGF+PNF RLG+WN IMFLT
Sbjct: 241 VCIGSPIDVVKSRMMGDSTYRNTVDCFIKTMKTEGIMAFYKGFLPNFTRLGTWNAIMFLT 294

BLAST of CmoCh03G003860 vs. TAIR 10
Match: AT5G58970.2 (uncoupling protein 2 )

HSP 1 Score: 358.6 bits (919), Expect = 5.0e-99
Identity = 181/250 (72.40%), Postives = 208/250 (83.20%), Query Frame = 0

Query: 2   VADSKGKSDISFAGTFASSAFAACFAELSNSNYNRGFVCTIPLDTAKVRLQLQKKAVAGD 61
           +AD K + +ISF  TF  SAFAACFAEL          CTIPLDTAKVRLQLQ+K   GD
Sbjct: 1   MADFKPRIEISFLETFICSAFAACFAEL----------CTIPLDTAKVRLQLQRKIPTGD 60

Query: 62  A--LPKYRGMLGTVATIAREEGLASLWKGIVPGLHRQCVYGGLRIGLYEPVKTFYVGSDF 121
              LPKYRG +GT+ATIAREEG++ LWKG++ GLHRQC+YGGLRIGLYEPVKT  VGSDF
Sbjct: 61  GENLPKYRGSIGTLATIAREEGISGLWKGVIAGLHRQCIYGGLRIGLYEPVKTLLVGSDF 120

Query: 122 VGDVPLSKKILAALTTG---ITIANPTDLVKVRLQAEGKLPPGVPRRYSGALSAYSTIVR 181
           +GD+PL +KILAAL TG   I +ANPTDLVKVRLQ+EGKLP GVPRRY+GA+ AY TIV+
Sbjct: 121 IGDIPLYQKILAALLTGAIAIIVANPTDLVKVRLQSEGKLPAGVPRRYAGAVDAYFTIVK 180

Query: 182 QEGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVITHLLAGLGAGFFA 241
            EGV ALWTG+GPNIARNAI+NAAELASYDQ+K+TI+KIP F D+V+THLLAGL AGFFA
Sbjct: 181 LEGVSALWTGLGPNIARNAIVNAAELASYDQIKETIMKIPFFRDSVLTHLLAGLAAGFFA 240

Query: 242 VCIGSPVDVV 247
           VCIGSP+DVV
Sbjct: 241 VCIGSPIDVV 240

BLAST of CmoCh03G003860 vs. TAIR 10
Match: AT1G14140.1 (Mitochondrial substrate carrier family protein )

HSP 1 Score: 188.0 bits (476), Expect = 1.2e-47
Identity = 104/273 (38.10%), Postives = 159/273 (58.24%), Query Frame = 0

Query: 41  TIPLDTAKVRLQLQKKAVAGDALPKYR-GMLGTVATIAREEGLASLWKGIVPGLHRQCVY 100
           T P+D  K R+QL     +G A   +R G  G V+ IAR+EG+  L+KG+ P + R   Y
Sbjct: 30  TFPIDLTKTRMQLHG---SGSASGAHRIGAFGVVSEIARKEGVIGLYKGLSPAIIRHLFY 89

Query: 101 GGLRIGLYEPVKTFYVGSDFVG--DVPLSKKILAALTTGI---TIANPTDLVKVRLQAEG 160
             +RI  YE +K   V S+      +PL+ K L    +G+    +A+P DLVKVR+QA+G
Sbjct: 90  TPIRIIGYENLKGLIVRSETNNSESLPLATKALVGGFSGVIAQVVASPADLVKVRMQADG 149

Query: 161 KL-PPGVPRRYSGALSAYSTIVRQEGVGALWTGIGPNIARNAIINAAELASYDQVKQTIL 220
           +L   G+  RYSG + A++ I++ EGV  LW G+ PNI R  ++N  ELA YD  K  ++
Sbjct: 150 RLVSQGLKPRYSGPIEAFTKILQSEGVKGLWKGVLPNIQRAFLVNMGELACYDHAKHFVI 209

Query: 221 KIPGFTDNVITHLLAGLGAGFFAVCIGSPVDVVKSRMMG---DSTYKNTLDCFIKTLRND 280
                 DN+  H LA + +G  +  +  P DVVK+RMM    ++ Y+N+ DC +KT++ +
Sbjct: 210 DKKIAEDNIFAHTLASIMSGLASTSLSCPADVVKTRMMNQGENAVYRNSYDCLVKTVKFE 269

Query: 281 GPLAFYKGFIPNFGRLGSWNVIMFLTLEQVFLV 304
           G  A +KGF P + RLG W  + +++ E+  L+
Sbjct: 270 GIRALWKGFFPTWARLGPWQFVFWVSYEKFRLL 299

BLAST of CmoCh03G003860 vs. TAIR 10
Match: AT2G22500.1 (uncoupling protein 5 )

HSP 1 Score: 180.6 bits (457), Expect = 1.9e-45
Identity = 105/286 (36.71%), Postives = 158/286 (55.24%), Query Frame = 0

Query: 41  TIPLDTAKVRLQLQ----------------KKAVAGDALPKYRGMLGTVATIAREEGLAS 100
           T PLD  KVR+QLQ                + +   +A P   G++G  + + REEG+ +
Sbjct: 20  THPLDLIKVRMQLQGESAPIQTNLRPALAFQTSTTVNAPPLRVGVIGVGSRLIREEGMRA 79

Query: 101 LWKGIVPGLHRQCVYGGLRIGLYEPVKTFYVGSDFVGDVPLSKKILA---ALTTGITIAN 160
           L+ G+   + RQ +Y   R+GLY+ +K  +   +    +PL KKI A   A   G  + N
Sbjct: 80  LFSGVSATVLRQTLYSTTRMGLYDIIKGEWTDPE-TKTMPLMKKIGAGAIAGAIGAAVGN 139

Query: 161 PTDLVKVRLQAEGKLPPGVPRRYSGALSAYSTIVRQEGVGALWTGIGPNIARNAIINAAE 220
           P D+  VR+QA+G+LP    R Y   L A + ++R EGV +LW G    I R  ++ +++
Sbjct: 140 PADVAMVRMQADGRLPLTDRRNYKSVLDAITQMIRGEGVTSLWRGSSLTINRAMLVTSSQ 199

Query: 221 LASYDQVKQTILKIPGFTDNVITHLLAGLGAGFFAVCIGSPVDVVKSRMMGDST------ 280
           LASYD VK+TIL+     D + TH+ A   AGF A    +PVDV+K+R+M          
Sbjct: 200 LASYDSVKETILEKGLLKDGLGTHVSASFAAGFVASVASNPVDVIKTRVMNMKVVAGVAP 259

Query: 281 -YKNTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQV 301
            YK  +DC +KT++ +G ++ YKGFIP   R   + V++F+TLEQV
Sbjct: 260 PYKGAVDCALKTVKAEGIMSLYKGFIPTVSRQAPFTVVLFVTLEQV 304

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O818454.2e-13581.21Mitochondrial uncoupling protein 1 OS=Arabidopsis thaliana OX=3702 GN=PUMP1 PE=1... [more]
Q9ZWG11.6e-12673.36Mitochondrial uncoupling protein 2 OS=Arabidopsis thaliana OX=3702 GN=PUMP2 PE=2... [more]
O976491.7e-6749.12Mitochondrial uncoupling protein 3 OS=Sus scrofa OX=9823 GN=UCP3 PE=2 SV=1[more]
P565012.2e-6747.77Mitochondrial uncoupling protein 3 OS=Mus musculus OX=10090 GN=Ucp3 PE=1 SV=1[more]
Q9N2I94.9e-6748.46Mitochondrial uncoupling protein 3 OS=Canis lupus familiaris OX=9615 GN=UCP3 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1IM651.4e-15795.70mitochondrial uncoupling protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111478... [more]
A0A6J1GFM71.4e-15795.70mitochondrial uncoupling protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A5A7TT482.5e-15491.00Mitochondrial uncoupling protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BLL82.5e-15491.00mitochondrial uncoupling protein 1 OS=Cucumis melo OX=3656 GN=LOC103490980 PE=3 ... [more]
A0A6J1HUA84.7e-15391.39mitochondrial uncoupling protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111466... [more]
Match NameE-valueIdentityDescription
XP_022950708.12.9e-15795.70mitochondrial uncoupling protein 1-like [Cucurbita moschata] >XP_022978241.1 mit... [more]
XP_023543070.18.4e-15795.36mitochondrial uncoupling protein 1-like [Cucurbita pepo subsp. pepo][more]
XP_038880991.13.9e-15491.00mitochondrial uncoupling protein 1 [Benincasa hispida][more]
XP_008448974.15.1e-15491.00PREDICTED: mitochondrial uncoupling protein 1 [Cucumis melo] >KAA0045067.1 mitoc... [more]
XP_022966614.19.7e-15391.39mitochondrial uncoupling protein 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT3G54110.13.0e-13681.21plant uncoupling mitochondrial protein 1 [more]
AT5G58970.11.1e-12773.36uncoupling protein 2 [more]
AT5G58970.25.0e-9972.40uncoupling protein 2 [more]
AT1G14140.11.2e-4738.10Mitochondrial substrate carrier family protein [more]
AT2G22500.11.9e-4536.71uncoupling protein 5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002030Mitochondrial carrier UCP-likePRINTSPR00784MTUNCOUPLINGcoord: 174..191
score: 50.0
coord: 18..30
score: 39.64
coord: 217..235
score: 41.7
IPR023395Mitochondrial carrier domain superfamilyGENE3D1.50.40.10Mitochondrial carrier domaincoord: 16..306
e-value: 3.2E-74
score: 251.4
IPR023395Mitochondrial carrier domain superfamilySUPERFAMILY103506Mitochondrial carriercoord: 38..300
IPR018108Mitochondrial substrate/solute carrierPFAMPF00153Mito_carrcoord: 221..300
e-value: 4.5E-17
score: 61.8
coord: 130..214
e-value: 4.8E-16
score: 58.5
coord: 39..115
e-value: 4.4E-15
score: 55.4
IPR018108Mitochondrial substrate/solute carrierPROSITEPS50920SOLCARcoord: 122..211
score: 24.765587
IPR018108Mitochondrial substrate/solute carrierPROSITEPS50920SOLCARcoord: 15..113
score: 24.658321
IPR018108Mitochondrial substrate/solute carrierPROSITEPS50920SOLCARcoord: 220..303
score: 25.881147
NoneNo IPR availablePANTHERPTHR45618MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATEDcoord: 4..302
NoneNo IPR availablePANTHERPTHR45618:SF12MITOCHONDRIAL UNCOUPLING PROTEIN 1coord: 4..302
NoneNo IPR availablePROSITEPS51257PROKAR_LIPOPROTEINcoord: 1..25
score: 5.0

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh03G003860.1CmoCh03G003860.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0003333 amino acid transmembrane transport
biological_process GO:1990542 mitochondrial transmembrane transport
biological_process GO:0006839 mitochondrial transport
biological_process GO:0009853 photorespiration
biological_process GO:1902600 proton transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005743 mitochondrial inner membrane
cellular_component GO:0031966 mitochondrial membrane
molecular_function GO:0015171 amino acid transmembrane transporter activity
molecular_function GO:0017077 oxidative phosphorylation uncoupler activity