CmoCh03G002730 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh03G002730
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	RING-type E3 ubiquitin transferase
Location	Cmo_Chr03: 3990423 .. 3992842 (-)
RNA-Seq Expression	CmoCh03G002730
Synteny	CmoCh03G002730

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AACTTCCCAACATCTCTCTCTCTCTCTCTCATGGCAGAATTAGATCAAGAACTTGATCCTCCTCCCTTCTTTCTTTGCCCCATCTCTCTCCAAATTATGAAAGACCCCGTCACGGTTGCTTCTGGCATAACCTACGACCGCGAAAGCATCGAGAGATGGTTGTTTTCAGATAGACATAAGACGTGTCCCATCACACAGGTCGTCTTGTCGAACTTCGACATCACTCCCAACCATACTCTAAGACGAGTCATTCAAGCTTGGTGTACTGTCAATGCTTGCAAGGGCGTCGAGAGGATTCCGACGCCTAAGCCACCGGTCGATTGTAAACAAGTGGTTCGAATTCTTGCAGATGCCAAGTTGTCTTCGTTCTCGCAACAGAATTGTCTCCGACGACTCCAATCTATTGCAGCGGCGAGTGAATCTAATAAACGTTGCATGGAAGCTGCTGGAGCTGTGGAGTTCTTGGCCGGAATTGTGTGTAATTCCACCAACGTGGAATTCAGCTTAGAAGGTATTATTTATACATATATAAATAATTATTAGATTTTTTTTTTTTAATATTAAGCTGATTAAAGTATTTAAAAAAAATAAAGGATTCGTTTTTAATTTTCAATATTGGGACGAAACAATTCAAGAAATTTAAAATTTCTTACAATTAATTTTAATTAATTTTTTTTTTAAATTTTGGATACTCTTCCATAATAGTTTTATAAGACATAAAATCTAATTTTATATATGATAAATTAGTTAATTTTTAAAATTTTAAAATATGACAATAATTTATTGAATAACAAAAACTGAAAGTTTAAAAAATTATTAAATTAATATAATATAACACTAAATTATAAGAAATATTTTAAATTTTGTCTGTGCTTCAAAAATACTTCTAGTTTCAAATTTGCAATGTTTATTTTTATATTTATATTTATATATAATTATTTGAATTTTCTTTTTTATTTTGAATATTAAGCTTATTAAAAAATTAATTTTTAAAATGTTAGGTCTTTTTTCAAAAATTGGATTTGTTTTTAATTTTAAAAAAAAACAAAAACTTTTTTTTTAATTTAACATTAAAAATTTAAAATTTCAGCAATTTTAAAAATTTCTTACACGCTATCTAATTAATTTTTTTATTTTAATAAGTAGATGGTTCAACATTTTTAAACCGAATTATTAAATTTTATGAGACATATTTATATTTAATAAATTAATTAATTTTTAAAATTTTTTAATATGTCAATAATTTATTAAATAAATAAATGAAAAGTTTAAAATCTATTAAATTAATATAATTTAAAACTAATTATAAGAAATATTTTAAATTTTGTGTGCGTTACATTTTTAATTTTAAAATTTGCAACGTTTTCTTTCGTTTTTTTTAGGGTTTCAAAAATTTCGAGTTTAAATAATTTAAAATTTTAAGCTAAAATTGAAATTTAAACGATTTACGAGTCTAAATCGTTTAGAATTTTGGATCAAAACGGAAATTTAAACGATTTAGGAGTGATTTCGAACGAAAACTTCAATTTCAATCCTAGAAGTCTTATTTTAAATTTGTAATTTGTGTAGTAAAAGTTGAGAATCTCTATTTGATGAACATTGTTGAGCAGATTACGCTTACTTGATTGACGACCAGTTGGCGAATTTGGGATTTGAGGCTTTGAGCATTCTTTACAAACTTCAAATCTCCGAATCCGGCCTGAAATTTCTACTCGCCAACAATGGCGGCATCTTCGTCTCAACCTTAACCAAAATCTTGCAAAACGGAACCTATGGCTCCCGAACATATTCAGTAATGCTTCTCACTTCAATGTTCGAAGTTGCAGATCAGATTCAGATCCTCAGCCTAAACACCGATTTCTTCGTCGAAATCGTCCAAATTCTGAAGGATCAGATCTCGAAACAGGCTTCGAAATCGGCATTGAAACTGCTAATCGGTCTTTGTTCGAGTTCCAGAAACAGAGTTAAGGCGGTCAAAGCCGGTGCGGTTCCGGTTCTGATCGATCTCCTCCTCGATCTCGATTCGTCAGAGAAGCGTTCGTGCGAGATGATTCTCGTGACGCTCGATCTACTCTGCGGCTGCGCCGACGGCAGGGCGGAGGTATTGAATCACGCGGCAGGGATCGCTATTGTGTCGAAGAAGATTCTGAGAGTTTCGACACTCGGAAGCGAAAAGGCCGTCGGAATCTTGTGCTCTATCGCGAGATTCTCGGCGAGTGAAAGCGTCGTCCAGGAAATGATGCGGCTCGGAGTTGTGACGAAGCTCTGTTTTCTGCTTCAGGTCGCCGGCGCCGGAGGCAAAGCGAAGGAGAAAGCGAAAGAGATTCTGAAGCTTCATGGTCGGACATGGCGGAATTCTTCATGTTTGCCTTCGAGTCTGCGATCTTCTTTTCCACATTAGGGTTTGTTTTTGCCCCCAA

mRNA sequence

AACTTCCCAACATCTCTCTCTCTCTCTCTCATGGCAGAATTAGATCAAGAACTTGATCCTCCTCCCTTCTTTCTTTGCCCCATCTCTCTCCAAATTATGAAAGACCCCGTCACGGTTGCTTCTGGCATAACCTACGACCGCGAAAGCATCGAGAGATGGTTGTTTTCAGATAGACATAAGACGTGTCCCATCACACAGGTCGTCTTGTCGAACTTCGACATCACTCCCAACCATACTCTAAGACGAGTCATTCAAGCTTGGTGTACTGTCAATGCTTGCAAGGGCGTCGAGAGGATTCCGACGCCTAAGCCACCGGTCGATTGTAAACAAGTGGTTCGAATTCTTGCAGATGCCAAGTTGTCTTCGTTCTCGCAACAGAATTGTCTCCGACGACTCCAATCTATTGCAGCGGCGAGTGAATCTAATAAACGTTGCATGGAAGCTGCTGGAGCTGTGGAGTTCTTGGCCGGAATTGTGTGTAATTCCACCAACGTGGAATTCAGCTTAGAAGATTACGCTTACTTGATTGACGACCAGTTGGCGAATTTGGGATTTGAGGCTTTGAGCATTCTTTACAAACTTCAAATCTCCGAATCCGGCCTGAAATTTCTACTCGCCAACAATGGCGGCATCTTCGTCTCAACCTTAACCAAAATCTTGCAAAACGGAACCTATGGCTCCCGAACATATTCAGTAATGCTTCTCACTTCAATGTTCGAAGTTGCAGATCAGATTCAGATCCTCAGCCTAAACACCGATTTCTTCGTCGAAATCGTCCAAATTCTGAAGGATCAGATCTCGAAACAGGCTTCGAAATCGGCATTGAAACTGCTAATCGGTCTTTGTTCGAGTTCCAGAAACAGAGTTAAGGCGGTCAAAGCCGGTGCGGTTCCGGTTCTGATCGATCTCCTCCTCGATCTCGATTCGTCAGAGAAGCGTTCGTGCGAGATGATTCTCGTGACGCTCGATCTACTCTGCGGCTGCGCCGACGGCAGGGCGGAGGTATTGAATCACGCGGCAGGGATCGCTATTGTGTCGAAGAAGATTCTGAGAGTTTCGACACTCGGAAGCGAAAAGGCCGTCGGAATCTTGTGCTCTATCGCGAGATTCTCGGCGAGTGAAAGCGTCGTCCAGGAAATGATGCGGCTCGGAGTTGTGACGAAGCTCTGTTTTCTGCTTCAGGTCGCCGGCGCCGGAGGCAAAGCGAAGGAGAAAGCGAAAGAGATTCTGAAGCTTCATGGTCGGACATGGCGGAATTCTTCATGTTTGCCTTCGAGTCTGCGATCTTCTTTTCCACATTAGGGTTTGTTTTTGCCCCCAA

Coding sequence (CDS)

ATGGCAGAATTAGATCAAGAACTTGATCCTCCTCCCTTCTTTCTTTGCCCCATCTCTCTCCAAATTATGAAAGACCCCGTCACGGTTGCTTCTGGCATAACCTACGACCGCGAAAGCATCGAGAGATGGTTGTTTTCAGATAGACATAAGACGTGTCCCATCACACAGGTCGTCTTGTCGAACTTCGACATCACTCCCAACCATACTCTAAGACGAGTCATTCAAGCTTGGTGTACTGTCAATGCTTGCAAGGGCGTCGAGAGGATTCCGACGCCTAAGCCACCGGTCGATTGTAAACAAGTGGTTCGAATTCTTGCAGATGCCAAGTTGTCTTCGTTCTCGCAACAGAATTGTCTCCGACGACTCCAATCTATTGCAGCGGCGAGTGAATCTAATAAACGTTGCATGGAAGCTGCTGGAGCTGTGGAGTTCTTGGCCGGAATTGTGTGTAATTCCACCAACGTGGAATTCAGCTTAGAAGATTACGCTTACTTGATTGACGACCAGTTGGCGAATTTGGGATTTGAGGCTTTGAGCATTCTTTACAAACTTCAAATCTCCGAATCCGGCCTGAAATTTCTACTCGCCAACAATGGCGGCATCTTCGTCTCAACCTTAACCAAAATCTTGCAAAACGGAACCTATGGCTCCCGAACATATTCAGTAATGCTTCTCACTTCAATGTTCGAAGTTGCAGATCAGATTCAGATCCTCAGCCTAAACACCGATTTCTTCGTCGAAATCGTCCAAATTCTGAAGGATCAGATCTCGAAACAGGCTTCGAAATCGGCATTGAAACTGCTAATCGGTCTTTGTTCGAGTTCCAGAAACAGAGTTAAGGCGGTCAAAGCCGGTGCGGTTCCGGTTCTGATCGATCTCCTCCTCGATCTCGATTCGTCAGAGAAGCGTTCGTGCGAGATGATTCTCGTGACGCTCGATCTACTCTGCGGCTGCGCCGACGGCAGGGCGGAGGTATTGAATCACGCGGCAGGGATCGCTATTGTGTCGAAGAAGATTCTGAGAGTTTCGACACTCGGAAGCGAAAAGGCCGTCGGAATCTTGTGCTCTATCGCGAGATTCTCGGCGAGTGAAAGCGTCGTCCAGGAAATGATGCGGCTCGGAGTTGTGACGAAGCTCTGTTTTCTGCTTCAGGTCGCCGGCGCCGGAGGCAAAGCGAAGGAGAAAGCGAAAGAGATTCTGAAGCTTCATGGTCGGACATGGCGGAATTCTTCATGTTTGCCTTCGAGTCTGCGATCTTCTTTTCCACATTAG

Protein sequence

MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLSNFDITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQNCLRRLQSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGFEALSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQILSLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARFSASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSSFPH

Homology

[Close]

BLAST of CmoCh03G002730 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 8.4e-118
Identity = 228/435 (52.41%), Postives = 307/435 (70.57%), Query Frame = 0


Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLSNFD 63
           +DQE++ P FFLCPISL IMKDPV V++GITYDRESIE+WLFS +  +CP+T+ V++  D
Sbjct: 1   MDQEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD 60

Query: 64  ITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQNCLRRLQ 123
           +TPNHTLRR+IQ+WCT+NA  G+ERIPTPKPP+   ++ +++ ++  S  +Q  CL+RL+
Sbjct: 61  LTPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLR 120

Query: 124 SIAAASESNKRCMEAAGAVEFLAGIVCNST---NVEFSLEDYAYLID-----------DQ 183
            I + + +NKRC+EAA   EFLA IV NS    N   S    + L D           D 
Sbjct: 121 QIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDS 180

Query: 184 LANLGFEALSILYKLQISESGLKFLLANNGGI-FVSTLTKILQNGTYGSRTYSVMLLTSM 243
             +L  EALS+LY L  SE+ LK LL N  G   V TLTKI+Q G Y SR Y+ +LL  +
Sbjct: 181 SRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKL 240

Query: 244 FEVADQIQILSLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVP 303
            EVAD +QI+ L  + F E++QIL DQIS +A++SA+++L+  C   RNR KAV+ G + 
Sbjct: 241 LEVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTIS 300

Query: 304 VLIDLLL-DLDSSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGS 363
           ++I+LL+ D  SSE+R+ EM +V LD+LC CA+GRAE LNH A IA+VSKKILRVS + S
Sbjct: 301 MIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITS 360

Query: 364 EKAVGILCSIARFSASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTW 423
           E+AV +L S+ RF A+ S++QEM++LGVV KLC +LQV+  G K KEKAKE+LKLH R W
Sbjct: 361 ERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVS-CGNKTKEKAKELLKLHARVW 420

[Close]

BLAST of CmoCh03G002730 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 417.5 bits (1072), Expect = 1.7e-115
Identity = 215/421 (51.07%), Postives = 306/421 (72.68%), Query Frame = 0


Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLSNFD 63
           +D+E++ PPFFLCPISL+IMKDPV V++GITYDR+SIE+WLF+ +  +CP+T+  +++ D
Sbjct: 6   MDEEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDAD 65

Query: 64  ITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQNCLRRLQ 123
           +TPNHTLRR+IQ+WCT+NA  GVERIPTP+PP+   ++ +++ D+  S  +Q  CL+RL+
Sbjct: 66  LTPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLR 125

Query: 124 SIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGFEALSILYK 183
            I + + +NKRC+EAAG  EFLA IV N               D +  +L  EAL++LY 
Sbjct: 126 QIVSENATNKRCLEAAGVPEFLANIVSN---------------DSENGSLTDEALNLLYH 185

Query: 184 LQISESGLKFLLAN-NGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQILSLNT 243
           L+ SE+ LK LL N      V +LTKI+Q G Y SR Y+ +LL ++ EVAD +Q ++L  
Sbjct: 186 LETSETVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP 245

Query: 244 DFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLD-SSE 303
           + F E+VQIL D+IS++A+K+A+ +L+ +C   RNR KAV+AG + V+I+LL+D   +SE
Sbjct: 246 EVFTEVVQILDDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSE 305

Query: 304 KRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARFS 363
           +R  EM +V LDLLC CA+GRAE LNH A IA+V KKILRVS   S++AV +L S+ RF 
Sbjct: 306 RRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFC 365

Query: 364 ASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSSF 423
           A+ +++ EM++LGVV KLC +LQV+  GGK KEKAKE+LKLH R W++S CLP ++  ++
Sbjct: 366 ATPALLHEMLQLGVVAKLCLVLQVS-CGGKTKEKAKELLKLHARVWKDSPCLPKNMILAY 410

[Close]

BLAST of CmoCh03G002730 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 8.8e-59
Identity = 158/456 (34.65%), Postives = 245/456 (53.73%), Query Frame = 0


Query: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLS 60
           M + ++E++ P +F+CPISL+IMKDPVT  SGITYDR++I +WL  ++  +CP+T+  L 
Sbjct: 1   MDQEEEEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLP 60

Query: 61  -NFDITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAK---LSSFSQQ 120
            + D+TPNH LRR+IQ WC  N  +GV RI TP+ P     VV  + + K     +  ++
Sbjct: 61  LDSDLTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGRE 120

Query: 121 NCLRRLQSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGFE 180
             L++L+ + A   +N+R M   G  + L   V   T+ +         +D        E
Sbjct: 121 ETLQKLE-VLAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLD--------E 180

Query: 181 ALSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQ 240
           +L +L+ + I  +  K +L  N  + + +LT +L    + S+ Y+++LL ++ E      
Sbjct: 181 SLRLLHLIGIPSNDAKTILMENDRV-MESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHI 240

Query: 241 ILSLNTDFFVEIVQILKDQIS-----------------------------------KQAS 300
           +  LN + F  I+  LKD ++                                   KQA 
Sbjct: 241 VERLNPEIFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAV 300

Query: 301 KSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSSEKRSCEMILVTLDLLCGCADG 360
            +AL +L+   S SRNR   V  GAV  LI+L +   + EKR  E++L  L  LC CA+G
Sbjct: 301 TAALMILLETSSWSRNRSLLVDLGAVSELIELEISY-TGEKRITELMLGVLSRLCCCANG 360

Query: 361 RAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARFSASESVVQEMMRLGVVTKLCF 418
           RAE+L H  GIA+V+K++LRVS    ++A+ IL ++++FS    VV+EM+ +G V KLC 
Sbjct: 361 RAEILAHRGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCS 420

[Close]

BLAST of CmoCh03G002730 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 4.9e-49
Identity = 149/424 (35.14%), Postives = 226/424 (53.30%), Query Frame = 0


Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLSNFD 63
           LD  +  P  F CPISL++M+DPVTV +G TYDR SIE W+    + TCP+T+  LS+F 
Sbjct: 8   LDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFT 67

Query: 64  ITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAK------LSSFSQQN 123
           + PNHTLRR+IQ WC  N   GVERIPTPK P D   V  +L+ A       +S  S+  
Sbjct: 68  LIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAA 127

Query: 124 CLRRLQSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGFEA 183
            LRRL+  A  S+ N+  + A  A E L  I+ + T                 + L  E+
Sbjct: 128 ALRRLRGFARDSDKNRVLIAAHNATEILIKILFSETT---------------SSELVSES 187

Query: 184 LSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLL---TSMFEVADQ 243
           L++L  L I+E      ++++ G  V  LT++L + +  +R  +  L+   ++  + AD 
Sbjct: 188 LALLVMLPITEPNQFVSISSDPG-RVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADL 247

Query: 244 IQILSLNTDFFVEIVQILKDQI-SKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLID- 303
              +S +   F  ++ +L++ I S++A K  +K L  LCS    R  A+ AGA  +LID 
Sbjct: 248 KGSISNSESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDR 307

Query: 304 LLLDLDSSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVG 363
           L  D D  +    E  L T++LLC   +G A    HA  + ++ K ILRVS   +E A G
Sbjct: 308 LAADFDRCD---TERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAG 367

Query: 364 ILCSIARFSASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSC 417
            L  +A  +A E   +E    GVV +L  ++Q +    +AK+KA+++LKL   +W + + 
Sbjct: 368 AL--LALCTAEERWREEAAGAGVVVQLLLMVQ-SECTERAKKKAQKLLKLLRDSWPDYNS 409

[Close]

BLAST of CmoCh03G002730 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 3.8e-46
Identity = 146/424 (34.43%), Postives = 226/424 (53.30%), Query Frame = 0


Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLSNFD 63
           LD  +  P  F CPISL +M DPVT+++G TYDR SI+ W+ +  + TCP+T+V LS+F 
Sbjct: 8   LDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWI-AMGNTTCPVTRVALSDFT 67

Query: 64  ITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAK------LSSFSQQN 123
           + PNHTLRR+IQ WC  N   GVERIPTPK P D   V  +L+ A       +S  S+  
Sbjct: 68  LIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAA 127

Query: 124 CLRRLQSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLAN-LGFE 183
            +RRL+ +A  SE N+  +    A E L  I+            +A +    L++ L  E
Sbjct: 128 AIRRLRGLARDSEKNRVLIAGHNAREILVRIL------------FADIETTSLSSELVSE 187

Query: 184 ALSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQ 243
           +L++L  L ++E+  + + ++     V  +T++L + +   R  +  L+  +   A  + 
Sbjct: 188 SLALLVLLHMTETECEAVASDPSR--VGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMD 247

Query: 244 ---ILSLNTDFFVEIVQILKDQI-SKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLID 303
              I+S +   F  ++ +LK+ I S++A K  +K +  LC   + R  A+ AGA  +LID
Sbjct: 248 LKLIISGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILID 307

Query: 304 -LLLDLDSSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAV 363
            L  D D  +    E  L T++LLC   +G A    HA  + ++ K ILRVS   +E A 
Sbjct: 308 RLAADFDRCD---TERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAA 367

Query: 364 GILCSIARFSASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSS 416
           G L  +A  +A E    E    G+VT+L  L+Q +    +AK KA+ +LKL   +W + S
Sbjct: 368 GAL--LALCTAEERCRDEAAAAGLVTQLLLLVQ-SDCTERAKRKAQMLLKLLRDSWPDDS 410

[Close]

BLAST of CmoCh03G002730 vs. ExPASy TrEMBL
Match: A0A6J1GGQ8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111453790 PE=4 SV=1)

HSP 1 Score: 813.5 bits (2100), Expect = 4.0e-232
Identity = 423/423 (100.00%), Postives = 423/423 (100.00%), Query Frame = 0


Query: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLS 60
           MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLS 60

Query: 61  NFDITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQNCLR 120
           NFDITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQNCLR
Sbjct: 61  NFDITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQNCLR 120

Query: 121 RLQSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGFEALSI 180
           RLQSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGFEALSI
Sbjct: 121 RLQSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGFEALSI 180

Query: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQILSL 240
           LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQILSL
Sbjct: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQILSL 240

Query: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSS 300
           NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSS
Sbjct: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSS 300

Query: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARF 360
           EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARF
Sbjct: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARF 360

Query: 361 SASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420
           SASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS
Sbjct: 361 SASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420

Query: 421 FPH 424
           FPH
Sbjct: 421 FPH 423

[Close]

BLAST of CmoCh03G002730 vs. ExPASy TrEMBL
Match: A0A6J1IKG7 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111478249 PE=4 SV=1)

HSP 1 Score: 791.6 bits (2043), Expect = 1.6e-225
Identity = 406/423 (95.98%), Postives = 417/423 (98.58%), Query Frame = 0


Query: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLS 60
           MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCP+TQVVLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLS 60

Query: 61  NFDITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQNCLR 120
           +FDITPNHT+RRVIQAWCT+NACKG+ERIPTPKPP+DCKQVVRILADAKLS FSQQNCLR
Sbjct: 61  SFDITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCLR 120

Query: 121 RLQSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGFEALSI 180
           RL+SIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQ ANLG EALSI
Sbjct: 121 RLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQFANLGDEALSI 180

Query: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQILSL 240
           LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSR YSVMLLTSMFEVADQIQILSL
Sbjct: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240

Query: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSS 300
           NTDFF+EIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAV+AGA+PVLIDLLLDLDSS
Sbjct: 241 NTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLDSS 300

Query: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARF 360
           EKRSCEMILVTLDLLC CADGRAEVLNHAAGIA+VSKKILRVSTLGSEKAVGILCSIARF
Sbjct: 301 EKRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARF 360

Query: 361 SASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420
           SASESVVQEMMRLGVVTKLCFLLQV GAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS
Sbjct: 361 SASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420

Query: 421 FPH 424
           FPH
Sbjct: 421 FPH 423

[Close]

BLAST of CmoCh03G002730 vs. ExPASy TrEMBL
Match: A0A6J1EEF1 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432478 PE=4 SV=1)

HSP 1 Score: 681.0 bits (1756), Expect = 3.1e-192
Identity = 355/416 (85.34%), Postives = 380/416 (91.35%), Query Frame = 0


Query: 7   ELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLSNFDITP 66
           E+DPPPFFLCPISLQIMKDPVTVASGITYDRE+IERWLFSD+H TCP+TQVVLS+FDITP
Sbjct: 3   EVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFSDKHTTCPVTQVVLSDFDITP 62

Query: 67  NHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQNCLRRLQSIA 126
           N+TL R+IQAWCTVN    VERIPTPKPPVD KQVV+ILAD KLS FSQQNCLRRL+SIA
Sbjct: 63  NNTLSRLIQAWCTVN--NRVERIPTPKPPVDKKQVVKILADVKLSPFSQQNCLRRLESIA 122

Query: 127 AASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGFEALSILYKLQI 186
           +A+E NKRCMEAAGAVEFL G+VCNS  +E S  D+A+  DD LANLG EALSILYKLQI
Sbjct: 123 SANEMNKRCMEAAGAVEFLVGVVCNSITIESSFPDFAF--DDPLANLGDEALSILYKLQI 182

Query: 187 SESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQILSLNTDFFV 246
           SESGLKFLLANNGGIFV+TLTK LQNGTYGSR Y+ MLL SMFEVAD+IQI SLN +FFV
Sbjct: 183 SESGLKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFV 242

Query: 247 EIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSSEKRSCE 306
           EIVQILKDQISKQASKSALKLLIGLCSS RNRV AV+AGAVPVLIDLLLDLDSSEKR CE
Sbjct: 243 EIVQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDSSEKRLCE 302

Query: 307 MILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARFSASESV 366
           MILV LDLLCGCADGRAE+LNHAAGIA+VSKKILRVS+LGSEKAVGILCSIARFSAS+SV
Sbjct: 303 MILVALDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSV 362

Query: 367 VQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSSFP 423
           VQEM RLGVV KLCFLLQV GAG KAKEKAKEILKLHGRTWRNSSC PSSLR S+P
Sbjct: 363 VQEMARLGVVVKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSLRPSYP 414

[Close]

BLAST of CmoCh03G002730 vs. ExPASy TrEMBL
Match: A0A5A7UVZ1 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold654G00730 PE=4 SV=1)

HSP 1 Score: 667.9 bits (1722), Expect = 2.7e-188
Identity = 350/427 (81.97%), Postives = 389/427 (91.10%), Query Frame = 0


Query: 1   MAEL---DQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQV 60
           MAEL   DQ +DPPP+FLCPISLQIMKDPVT+ASGITYDRESIE+WLFSD+HKTCP++ +
Sbjct: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60

Query: 61  VLSNFDITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQN 120
           VLS+FD+TPNHTLRRVIQAWCTVNA KGVERIPTPKPPVD +QVVRILADAKLS FSQ+N
Sbjct: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120

Query: 121 CLRRLQSIAAASESNKRCMEAAGAVEFLAGIVCNS-TNVEFSLEDYAYLIDDQLANLGFE 180
           CL RL+SIAAA+ESNKRCME+AG VEFLAG+VCNS TN+E+ LEDYA+  DD  AN   E
Sbjct: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAF--DDMSANSADE 180

Query: 181 ALSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQ 240
           AL IL+KLQISES LKFLL+NNGGIF+STLTKILQN +Y SR+YSVMLL SMFEVADQIQ
Sbjct: 181 ALIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQ 240

Query: 241 ILSLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLD 300
           I +L  DFF+EI+QILKDQISKQASKSALKLLI + SS RNRVKAV+AGAVPVLIDLLLD
Sbjct: 241 IQNLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLD 300

Query: 301 LDSSE-KRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILC 360
           LDSSE KR CEMILV LD LCGCADGRAE+LNHAAGIA+VSKKILR+ST+GSEKAVGIL 
Sbjct: 301 LDSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILW 360

Query: 361 SIARFSASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPS 420
           SIARFS S+SVVQEM+R+GVVTKLCFLLQV GAG KAKEKAKEILKLHGRTWRNSSCLPS
Sbjct: 361 SIARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPS 420

Query: 421 SLRSSFP 423
           +LRS++P
Sbjct: 421 TLRSAYP 425

[Close]

BLAST of CmoCh03G002730 vs. ExPASy TrEMBL
Match: A0A1S3B4K2 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103485961 PE=4 SV=1)

HSP 1 Score: 667.9 bits (1722), Expect = 2.7e-188
Identity = 350/427 (81.97%), Postives = 389/427 (91.10%), Query Frame = 0


Query: 1   MAEL---DQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQV 60
           MAEL   DQ +DPPP+FLCPISLQIMKDPVT+ASGITYDRESIE+WLFSD+HKTCP++ +
Sbjct: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60

Query: 61  VLSNFDITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQN 120
           VLS+FD+TPNHTLRRVIQAWCTVNA KGVERIPTPKPPVD +QVVRILADAKLS FSQ+N
Sbjct: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120

Query: 121 CLRRLQSIAAASESNKRCMEAAGAVEFLAGIVCNS-TNVEFSLEDYAYLIDDQLANLGFE 180
           CL RL+SIAAA+ESNKRCME+AG VEFLAG+VCNS TN+E+ LEDYA+  DD  AN   E
Sbjct: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAF--DDMSANSADE 180

Query: 181 ALSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQ 240
           AL IL+KLQISES LKFLL+NNGGIF+STLTKILQN +Y SR+YSVMLL SMFEVADQIQ
Sbjct: 181 ALIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQ 240

Query: 241 ILSLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLD 300
           I +L  DFF+EI+QILKDQISKQASKSALKLLI + SS RNRVKAV+AGAVPVLIDLLLD
Sbjct: 241 IQNLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLD 300

Query: 301 LDSSE-KRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILC 360
           LDSSE KR CEMILV LD LCGCADGRAE+LNHAAGIA+VSKKILR+ST+GSEKAVGIL 
Sbjct: 301 LDSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILW 360

Query: 361 SIARFSASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPS 420
           SIARFS S+SVVQEM+R+GVVTKLCFLLQV GAG KAKEKAKEILKLHGRTWRNSSCLPS
Sbjct: 361 SIARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPS 420

Query: 421 SLRSSFP 423
           +LRS++P
Sbjct: 421 TLRSAYP 425

[Close]

BLAST of CmoCh03G002730 vs. NCBI nr
Match: XP_022950790.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita moschata])

HSP 1 Score: 813.5 bits (2100), Expect = 8.4e-232
Identity = 423/423 (100.00%), Postives = 423/423 (100.00%), Query Frame = 0


Query: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLS 60
           MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLS 60

Query: 61  NFDITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQNCLR 120
           NFDITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQNCLR
Sbjct: 61  NFDITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQNCLR 120

Query: 121 RLQSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGFEALSI 180
           RLQSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGFEALSI
Sbjct: 121 RLQSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGFEALSI 180

Query: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQILSL 240
           LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQILSL
Sbjct: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQILSL 240

Query: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSS 300
           NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSS
Sbjct: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSS 300

Query: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARF 360
           EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARF
Sbjct: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARF 360

Query: 361 SASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420
           SASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS
Sbjct: 361 SASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420

Query: 421 FPH 424
           FPH
Sbjct: 421 FPH 423

[Close]

BLAST of CmoCh03G002730 vs. NCBI nr
Match: XP_023543358.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 792.7 bits (2046), Expect = 1.5e-225
Identity = 409/423 (96.69%), Postives = 416/423 (98.35%), Query Frame = 0


Query: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLS 60
           MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIE+WLFSDRHKTCP+TQVVLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQVVLS 60

Query: 61  NFDITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQNCLR 120
           +FDITPNHTLRRVIQAWCTVNACKG+ERIPTPKPPVDCKQVVRILADAKLS FSQQNCLR
Sbjct: 61  SFDITPNHTLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLR 120

Query: 121 RLQSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGFEALSI 180
           RL+SIAA SESNKRCMEAAGAVEFLAGIVCNSTNVEFS EDYAYLIDDQ ANLG EALSI
Sbjct: 121 RLRSIAAGSESNKRCMEAAGAVEFLAGIVCNSTNVEFSSEDYAYLIDDQFANLGDEALSI 180

Query: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQILSL 240
           LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSR YSVMLLTSMFEVADQIQILSL
Sbjct: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240

Query: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSS 300
           NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPV+IDLLLDLDSS
Sbjct: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVMIDLLLDLDSS 300

Query: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARF 360
           EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIA+VSKKILRVSTLGSEKAVGILCSIARF
Sbjct: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARF 360

Query: 361 SASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420
           SASESVVQEMMRLGVVTKLCFLLQV GAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS
Sbjct: 361 SASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420

Query: 421 FPH 424
           FPH
Sbjct: 421 FPH 423

[Close]

BLAST of CmoCh03G002730 vs. NCBI nr
Match: XP_022978192.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita maxima])

HSP 1 Score: 791.6 bits (2043), Expect = 3.4e-225
Identity = 406/423 (95.98%), Postives = 417/423 (98.58%), Query Frame = 0


Query: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLS 60
           MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCP+TQVVLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLS 60

Query: 61  NFDITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQNCLR 120
           +FDITPNHT+RRVIQAWCT+NACKG+ERIPTPKPP+DCKQVVRILADAKLS FSQQNCLR
Sbjct: 61  SFDITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCLR 120

Query: 121 RLQSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGFEALSI 180
           RL+SIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQ ANLG EALSI
Sbjct: 121 RLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQFANLGDEALSI 180

Query: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQILSL 240
           LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSR YSVMLLTSMFEVADQIQILSL
Sbjct: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240

Query: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSS 300
           NTDFF+EIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAV+AGA+PVLIDLLLDLDSS
Sbjct: 241 NTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLDSS 300

Query: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARF 360
           EKRSCEMILVTLDLLC CADGRAEVLNHAAGIA+VSKKILRVSTLGSEKAVGILCSIARF
Sbjct: 301 EKRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARF 360

Query: 361 SASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420
           SASESVVQEMMRLGVVTKLCFLLQV GAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS
Sbjct: 361 SASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420

Query: 421 FPH 424
           FPH
Sbjct: 421 FPH 423

[Close]

BLAST of CmoCh03G002730 vs. NCBI nr
Match: KAG7033684.1 (E3 ubiquitin-protein ligase PUB22, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 790.4 bits (2040), Expect = 7.6e-225
Identity = 411/423 (97.16%), Postives = 418/423 (98.82%), Query Frame = 0


Query: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLS 60
           MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDR+SIE+WLFSD HKTCP+TQVVLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRKSIEKWLFSDGHKTCPVTQVVLS 60

Query: 61  NFDITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQNCLR 120
           +FDITPN TLRRVIQAWCTVNACKG+ERIPTPKPPVDCKQVVRILADAKLS FSQQNCLR
Sbjct: 61  SFDITPNITLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLR 120

Query: 121 RLQSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGFEALSI 180
           RL+SIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLG EALSI
Sbjct: 121 RLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGDEALSI 180

Query: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQILSL 240
           L+KLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSR YSVMLLTSMFEVADQIQILSL
Sbjct: 181 LHKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240

Query: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSS 300
           NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSS
Sbjct: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSS 300

Query: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARF 360
           EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARF
Sbjct: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARF 360

Query: 361 SASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420
           SASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS
Sbjct: 361 SASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420

Query: 421 FPH 424
           FPH
Sbjct: 421 FPH 423

[Close]

BLAST of CmoCh03G002730 vs. NCBI nr
Match: KAG6603504.1 (E3 ubiquitin-protein ligase PUB22, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 743.8 bits (1919), Expect = 8.1e-211
Identity = 387/401 (96.51%), Postives = 395/401 (98.50%), Query Frame = 0


Query: 23  MKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLSNFDITPNHTLRRVIQAWCTVNA 82
           MKDPVTVASGITYDRESIE+WLFSD HKTCP+TQVVLS+FDITPN TLRRVIQAWCTVNA
Sbjct: 1   MKDPVTVASGITYDRESIEKWLFSDGHKTCPVTQVVLSSFDITPNITLRRVIQAWCTVNA 60

Query: 83  CKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQNCLRRLQSIAAASESNKRCMEAAGAV 142
           CKG+ERIPTPKPPVDCKQVVRILADAKLS FSQQNCLRRL+SIAAASESNKRCMEAAGAV
Sbjct: 61  CKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLRRLRSIAAASESNKRCMEAAGAV 120

Query: 143 EFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGFEALSILYKLQISESGLKFLLANNGGIF 202
           EFLAGIVCNSTNVEFSLEDYAYLIDDQLANLG EALSILYKLQISESGLKFLLANNGGIF
Sbjct: 121 EFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGDEALSILYKLQISESGLKFLLANNGGIF 180

Query: 203 VSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQILSLNTDFFVEIVQILKDQISKQASK 262
           V+TLTKILQNGTYGSR YSVMLLTSMFEVADQIQI+SLNTD F+EIVQILKDQISKQASK
Sbjct: 181 VTTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQIVSLNTDIFIEIVQILKDQISKQASK 240

Query: 263 SALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSSEKRSCEMILVTLDLLCGCADGR 322
           SALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSSEKRSCEMILVTLDLLCGCADGR
Sbjct: 241 SALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSSEKRSCEMILVTLDLLCGCADGR 300

Query: 323 AEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARFSASESVVQEMMRLGVVTKLCFL 382
           AEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARFSASESVVQEMMRLGVVTKLCFL
Sbjct: 301 AEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARFSASESVVQEMMRLGVVTKLCFL 360

Query: 383 LQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSSFPH 424
           LQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSSFPH
Sbjct: 361 LQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSSFPH 401

[Close]

BLAST of CmoCh03G002730 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 425.2 bits (1092), Expect = 6.0e-119
Identity = 228/435 (52.41%), Postives = 307/435 (70.57%), Query Frame = 0


Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLSNFD 63
           +DQE++ P FFLCPISL IMKDPV V++GITYDRESIE+WLFS +  +CP+T+ V++  D
Sbjct: 1   MDQEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD 60

Query: 64  ITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQNCLRRLQ 123
           +TPNHTLRR+IQ+WCT+NA  G+ERIPTPKPP+   ++ +++ ++  S  +Q  CL+RL+
Sbjct: 61  LTPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLR 120

Query: 124 SIAAASESNKRCMEAAGAVEFLAGIVCNST---NVEFSLEDYAYLID-----------DQ 183
            I + + +NKRC+EAA   EFLA IV NS    N   S    + L D           D 
Sbjct: 121 QIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDS 180

Query: 184 LANLGFEALSILYKLQISESGLKFLLANNGGI-FVSTLTKILQNGTYGSRTYSVMLLTSM 243
             +L  EALS+LY L  SE+ LK LL N  G   V TLTKI+Q G Y SR Y+ +LL  +
Sbjct: 181 SRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKL 240

Query: 244 FEVADQIQILSLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVP 303
            EVAD +QI+ L  + F E++QIL DQIS +A++SA+++L+  C   RNR KAV+ G + 
Sbjct: 241 LEVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTIS 300

Query: 304 VLIDLLL-DLDSSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGS 363
           ++I+LL+ D  SSE+R+ EM +V LD+LC CA+GRAE LNH A IA+VSKKILRVS + S
Sbjct: 301 MIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITS 360

Query: 364 EKAVGILCSIARFSASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTW 423
           E+AV +L S+ RF A+ S++QEM++LGVV KLC +LQV+  G K KEKAKE+LKLH R W
Sbjct: 361 ERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVS-CGNKTKEKAKELLKLHARVW 420

[Close]

BLAST of CmoCh03G002730 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 417.5 bits (1072), Expect = 1.2e-116
Identity = 215/421 (51.07%), Postives = 306/421 (72.68%), Query Frame = 0


Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLSNFD 63
           +D+E++ PPFFLCPISL+IMKDPV V++GITYDR+SIE+WLF+ +  +CP+T+  +++ D
Sbjct: 6   MDEEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDAD 65

Query: 64  ITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQNCLRRLQ 123
           +TPNHTLRR+IQ+WCT+NA  GVERIPTP+PP+   ++ +++ D+  S  +Q  CL+RL+
Sbjct: 66  LTPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLR 125

Query: 124 SIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGFEALSILYK 183
            I + + +NKRC+EAAG  EFLA IV N               D +  +L  EAL++LY 
Sbjct: 126 QIVSENATNKRCLEAAGVPEFLANIVSN---------------DSENGSLTDEALNLLYH 185

Query: 184 LQISESGLKFLLAN-NGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQILSLNT 243
           L+ SE+ LK LL N      V +LTKI+Q G Y SR Y+ +LL ++ EVAD +Q ++L  
Sbjct: 186 LETSETVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP 245

Query: 244 DFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLD-SSE 303
           + F E+VQIL D+IS++A+K+A+ +L+ +C   RNR KAV+AG + V+I+LL+D   +SE
Sbjct: 246 EVFTEVVQILDDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSE 305

Query: 304 KRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARFS 363
           +R  EM +V LDLLC CA+GRAE LNH A IA+V KKILRVS   S++AV +L S+ RF 
Sbjct: 306 RRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFC 365

Query: 364 ASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSSF 423
           A+ +++ EM++LGVV KLC +LQV+  GGK KEKAKE+LKLH R W++S CLP ++  ++
Sbjct: 366 ATPALLHEMLQLGVVAKLCLVLQVS-CGGKTKEKAKELLKLHARVWKDSPCLPKNMILAY 410

[Close]

BLAST of CmoCh03G002730 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 229.2 bits (583), Expect = 6.3e-60
Identity = 158/456 (34.65%), Postives = 245/456 (53.73%), Query Frame = 0


Query: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLS 60
           M + ++E++ P +F+CPISL+IMKDPVT  SGITYDR++I +WL  ++  +CP+T+  L 
Sbjct: 15  MDQEEEEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLP 74

Query: 61  -NFDITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAK---LSSFSQQ 120
            + D+TPNH LRR+IQ WC  N  +GV RI TP+ P     VV  + + K     +  ++
Sbjct: 75  LDSDLTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGRE 134

Query: 121 NCLRRLQSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGFE 180
             L++L+ + A   +N+R M   G  + L   V   T+ +         +D        E
Sbjct: 135 ETLQKLE-VLAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLD--------E 194

Query: 181 ALSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQ 240
           +L +L+ + I  +  K +L  N  + + +LT +L    + S+ Y+++LL ++ E      
Sbjct: 195 SLRLLHLIGIPSNDAKTILMENDRV-MESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHI 254

Query: 241 ILSLNTDFFVEIVQILKDQIS-----------------------------------KQAS 300
           +  LN + F  I+  LKD ++                                   KQA 
Sbjct: 255 VERLNPEIFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAV 314

Query: 301 KSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSSEKRSCEMILVTLDLLCGCADG 360
            +AL +L+   S SRNR   V  GAV  LI+L +   + EKR  E++L  L  LC CA+G
Sbjct: 315 TAALMILLETSSWSRNRSLLVDLGAVSELIELEISY-TGEKRITELMLGVLSRLCCCANG 374

Query: 361 RAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARFSASESVVQEMMRLGVVTKLCF 418
           RAE+L H  GIA+V+K++LRVS    ++A+ IL ++++FS    VV+EM+ +G V KLC 
Sbjct: 375 RAEILAHRGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCS 434

[Close]

BLAST of CmoCh03G002730 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 196.8 bits (499), Expect = 3.4e-50
Identity = 149/424 (35.14%), Postives = 226/424 (53.30%), Query Frame = 0


Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLSNFD 63
           LD  +  P  F CPISL++M+DPVTV +G TYDR SIE W+    + TCP+T+  LS+F 
Sbjct: 8   LDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFT 67

Query: 64  ITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAK------LSSFSQQN 123
           + PNHTLRR+IQ WC  N   GVERIPTPK P D   V  +L+ A       +S  S+  
Sbjct: 68  LIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAA 127

Query: 124 CLRRLQSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGFEA 183
            LRRL+  A  S+ N+  + A  A E L  I+ + T                 + L  E+
Sbjct: 128 ALRRLRGFARDSDKNRVLIAAHNATEILIKILFSETT---------------SSELVSES 187

Query: 184 LSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLL---TSMFEVADQ 243
           L++L  L I+E      ++++ G  V  LT++L + +  +R  +  L+   ++  + AD 
Sbjct: 188 LALLVMLPITEPNQFVSISSDPG-RVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADL 247

Query: 244 IQILSLNTDFFVEIVQILKDQI-SKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLID- 303
              +S +   F  ++ +L++ I S++A K  +K L  LCS    R  A+ AGA  +LID 
Sbjct: 248 KGSISNSESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDR 307

Query: 304 LLLDLDSSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVG 363
           L  D D  +    E  L T++LLC   +G A    HA  + ++ K ILRVS   +E A G
Sbjct: 308 LAADFDRCD---TERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAG 367

Query: 364 ILCSIARFSASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSC 417
            L  +A  +A E   +E    GVV +L  ++Q +    +AK+KA+++LKL   +W + + 
Sbjct: 368 AL--LALCTAEERWREEAAGAGVVVQLLLMVQ-SECTERAKKKAQKLLKLLRDSWPDYNS 409

[Close]

BLAST of CmoCh03G002730 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 187.2 bits (474), Expect = 2.7e-47
Identity = 146/424 (34.43%), Postives = 226/424 (53.30%), Query Frame = 0


Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLSNFD 63
           LD  +  P  F CPISL +M DPVT+++G TYDR SI+ W+ +  + TCP+T+V LS+F 
Sbjct: 8   LDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWI-AMGNTTCPVTRVALSDFT 67

Query: 64  ITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAK------LSSFSQQN 123
           + PNHTLRR+IQ WC  N   GVERIPTPK P D   V  +L+ A       +S  S+  
Sbjct: 68  LIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAA 127

Query: 124 CLRRLQSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLAN-LGFE 183
            +RRL+ +A  SE N+  +    A E L  I+            +A +    L++ L  E
Sbjct: 128 AIRRLRGLARDSEKNRVLIAGHNAREILVRIL------------FADIETTSLSSELVSE 187

Query: 184 ALSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQ 243
           +L++L  L ++E+  + + ++     V  +T++L + +   R  +  L+  +   A  + 
Sbjct: 188 SLALLVLLHMTETECEAVASDPSR--VGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMD 247

Query: 244 ---ILSLNTDFFVEIVQILKDQI-SKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLID 303
              I+S +   F  ++ +LK+ I S++A K  +K +  LC   + R  A+ AGA  +LID
Sbjct: 248 LKLIISGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILID 307

Query: 304 -LLLDLDSSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAV 363
            L  D D  +    E  L T++LLC   +G A    HA  + ++ K ILRVS   +E A 
Sbjct: 308 RLAADFDRCD---TERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAA 367

Query: 364 GILCSIARFSASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSS 416
           G L  +A  +A E    E    G+VT+L  L+Q +    +AK KA+ +LKL   +W + S
Sbjct: 368 GAL--LALCTAEERCRDEAAAAGLVTQLLLLVQ-SDCTERAKRKAQMLLKLLRDSWPDDS 410

The following BLAST results are available for this feature:

BLAST of CmoCh03G002730 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SVC6	8.4e-118	52.41	E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ...	[more]
Q84TG3	1.7e-115	51.07	E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ...	[more]
Q9SF15	8.8e-59	34.65	E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ...	[more]
Q9LT79	4.9e-49	35.14	U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2...	[more]
Q9FXA4	3.8e-46	34.43	U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2...	[more]

BLAST of CmoCh03G002730 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GGQ8	4.0e-232	100.00	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111453790...	[more]
A0A6J1IKG7	1.6e-225	95.98	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111478249 P...	[more]
A0A6J1EEF1	3.1e-192	85.34	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432478...	[more]
A0A5A7UVZ1	2.7e-188	81.97	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3B4K2	2.7e-188	81.97	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103485961 PE=4 ...	[more]

BLAST of CmoCh03G002730 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022950790.1	8.4e-232	100.00	E3 ubiquitin-protein ligase PUB22-like [Cucurbita moschata]	[more]
XP_023543358.1	1.5e-225	96.69	E3 ubiquitin-protein ligase PUB22-like [Cucurbita pepo subsp. pepo]	[more]
XP_022978192.1	3.4e-225	95.98	E3 ubiquitin-protein ligase PUB22-like [Cucurbita maxima]	[more]
KAG7033684.1	7.6e-225	97.16	E3 ubiquitin-protein ligase PUB22, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6603504.1	8.1e-211	96.51	E3 ubiquitin-protein ligase PUB22, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of CmoCh03G002730 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G52450.1	6.0e-119	52.41	plant U-box 22	[more]
AT2G35930.1	1.2e-116	51.07	plant U-box 23	[more]
AT3G11840.1	6.3e-60	34.65	plant U-box 24	[more]
AT3G19380.1	3.4e-50	35.14	plant U-box 25	[more]
AT1G49780.1	2.7e-47	34.43	plant U-box 26	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003613	U box domain	SMART	SM00504	Ubox_2	coord: 13..77 e-value: 3.1E-33 score: 126.4
IPR003613	U box domain	PFAM	PF04564	U-box	coord: 11..79 e-value: 9.9E-21 score: 73.7
IPR003613	U box domain	PROSITE	PS51698	U_BOX	coord: 9..84 score: 41.744625
IPR011989	Armadillo-like helical	GENE3D	1.25.10.10		coord: 98..405 e-value: 1.3E-15 score: 59.1
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 1..87 e-value: 3.1E-28 score: 99.7
None	No IPR available	PANTHER	PTHR22849	WDSAM1 PROTEIN	coord: 7..422
None	No IPR available	PANTHER	PTHR22849:SF132	E3 UBIQUITIN-PROTEIN LIGASE PUB23	coord: 7..422
None	No IPR available	CDD	cd16664	RING-Ubox_PUB	coord: 14..56 e-value: 1.41986E-21 score: 84.9778
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 7..85
IPR016024	Armadillo-type fold	SUPERFAMILY	48371	ARM repeat	coord: 104..385

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh03G002730.1	CmoCh03G002730.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0004842	ubiquitin-protein transferase activity