CmoCh03G001720 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh03G001720
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionPyrroline-5-carboxylate reductase
LocationCmo_Chr03: 2564720 .. 2570616 (+)
RNA-Seq ExpressionCmoCh03G001720
SyntenyCmoCh03G001720
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGCCAAAATGGATCCTCCTTCTTCAAAACAAACGGGATTTCGCGGTTGAGCCGTTAGATGTGATAGCGTAGCCAGTAGTAGCCGGAGGAGAGCTTTGGATCGGAATCAATGGAGTTCCTTCCGATTTCACTTGATGCTTACAGATTAGGGTTCATCGGAGCTGGAAAAATGGCGGAGAGTCTAGCAAGGGGAATCGTCCAATCTGGTCTCTTACCTCCTTCTCGAATCTCCACGGCTCTCTACCCCACTCCTGGCCCCCCCATTGATTTCGAATCTTTCGGTGTTCGAGTCCTCCCCAAGAACGAAAATGTATGGTTATGCTTCATCTTCATCTTTCTACTGCATCGCCATAATCGCGCTTTACGTTTTCTTTTTTCATCGATTATGTCCAGGTGGTTGAAGAAAGTGATGTGGTCATCTTCTCTGTAAAGCCTCAAATCGGTACGGCGCTTGTTCATCTCATCTACTCTTGTCCTGTTTTCCCCATTTGTTTATTCATTCATTTTTCTCCTTGGTATTCTCGTTGTCCTCTTGAAAAATATTTAAAAAAATTAATTGAGGAATGTCTCTGGTGATTTATATCTCGAAATGCTAAGTTTTCTTGTGTGATTTATTGGTATATAATGATTTGTCTTTTGATGAACTTTATGTTAAATCGTTCTGTGATGGGAAATACTGGTTGCAGTTAAGAATGTGGTGTTGAAGCTGAGGCCTCTACTGGGGAAGAAGCTTTTGGTTTCGATTGCTGCTGGGATAAAATTGAAGGATCTGCAGGTTCTTCACTTTACTAACTTGTTTTTTTGGATTTTCTGGTGGATGAGATTTTCTTGAGTTGAGTGTTCTGTGGTAAAACCGCAGCCTCAGGTATTCTGATGCATATAGCTTTGATTATCATATCAATTTTGGGGTTTCTCTTTGAATAATTTTGCCTGAAATTTTTTTATGGAAGGAAGTTTTTACTGATTTCTTTTGCTGTGAGGGGTAAGTTTGACGTGATTTAGATTCCACCTTGGTTGGAGAGGAAATGAAGCATTCCTTATAAGGATGTAGAAACCTCTCCCTAGTGAAACGCATTTTAAAATCTTGAGGGGAAGCCAGGAAGGGAAACCCCAAAGAGGATCATATCTTCTAACGGTGGGCTTGGGCTACTACAAATGGTATCAGAACTAGACACTGAGCGGTGTGCCAGCGTCCTGTCCCAAGGAGGTGGATTGTGAGATTCCACATTGGTTGGAGAGGGGAACAAAACATTCCTTATAAAGGATGGGGAAACCTCTCTCTAGTGGACGCGTTTTAAAACCTTGAAGGGAAGCACGGAAGGGAAAGCCCAAAGAAGATAATATCTACTCACATTTGACCCGTTAAAACCTTTTGTAGTGTTTGGTTGAATGGTTAAAAGTAAGTTCATTAATTCTCAAATCATTCTGAAGCATTTCAAAGTACTTGAACAATAAGCACCTGGGATGAATTTGTGATTACTTTTAGAATGGTGAGTTTGTGATTATCTGGGATGTTTCAAAATTTTCGAAGTGTTTGGTTAGATAATTAAGTGTTTGATTACAAGTTCTTGAAGTGATTTGATGAGAAGCACTTAATTAGTGTTTCTTTCAAGAGTCTTTATAGAAGAAGTGTTATGATTTAAAAGCACTATTTTCCTACAAAAGCTTGAATTAAGTCACATACTTGTTCCATTTATTCTTCCTTTTTTCTTTAGAATATACCTTTTGTTCTTTCTTTTCTACGTACAAATTGCAGTTGGAGTAATGCTTTATACATTCAGGATTGGGCAGGTCATAACCGATTCATTAGAGTTATGCCAAATACTCCAGCAGCTGTTGGAGAGGCTGCATCAGGTATTATCTTTTTTTCCTCTTTGCCTTAAATTTAATGTCATTTCTCTCTCTCATATATGTTTCTATAATGCTGAAATAAGTATTACTTCTTAGAGATGATAAAATTGATTTGGCTGGTATTTAATTAGAGGCTAAATTAACAAAAATACTTCGAAACTCTGTATTATTTTGTAAAAAAAAGACGGTAACTTTCAAAAATTTTAATAGTACCCTTAAACTTTTAAAAAAGTTTCAAAAATATTTTAGTGTTTTCTTTCAAAAATACCCCTTGAATTTCAAAAGTGTTTTGTAAATGCTATGAAAGCTTATTAAGTTTCATTAATACCTTTCAACGTTTTTAAAACGTTAAAAAATATTATTCCCGTTAGTACCATATTTAAAAAATACTTGTAAACTTTTAAAAGTAGCATTGACATCTTTAATCTTTGTAAGAAAAAAGTTTTAATAAGTTCTTACTATCCATGTGTGGATTGAAACCATCTATTTACACTTTGTAATCATAATAATTTAAATTTTTAAAATCATTTCTATAAATTTAAAGACATTAATGTTACTTTTGAAAGTTCATGAATATTTTTGAAGTAGGGTGATAACGGTTTTGTCCACGTGCTAACACTCAGCATTTTTTAATTTTTTAGAAAAGTTTAAGAGTATTAATAAAACTTTTGAATTTTTAAACTACTCTTAAAAATTCAATGGTGTTTTTGAAATACTCACGAAAGTTGATGAGTATTTTTGAAATTTTTTTTATAGGTCACGGGTATTTTATCCAAAACTAACCACACTAGCATAAGAGGATTTTTGAACATTTTTTTAAAAAGTTTAAGTATGTTTCTAACTTTCTTGTGAAAGTTTAAAGATATTGTGAAACTGAGAATATTTTGAAATATAGATGAATATTTTATTGATTAGCCTTAATTATATTATAATGTATGTTTTAGCGGTCACAACTGCTAATGGAACTAGGGATCCAATTAATTGGCCAATCATGGTGCAGGTTTTGAATTACATTTGCTGAATCTTTTTTGAAATCCTACACTTAATGAATCGTTCCATTACAAATCATATAAGAAAAAAGATTTAGGCATCTTGGAACTTTAAATAAATAAAAAAACAAAAAGAAAATAATTTGAGGATGAAGGTTTCTTCATATGCTAGCCTCAATCTAGACAATGCAGCTTCTTTCTGTTTAAATACTTTCATGTTCTGCAAAATTTTGTTCAACTTTCTAATTGATAGTCTGAATTACTTGGAAGCGACCCAAACTCGCAAAATATACTCACTAACTTCAGTACTACTTTCATTACATGATGTGGATGACAGATATCAAGATTTTCAATTCGTCTTGCTCCGTGCGTAGCATGGAGAAGAACAAAAGATACATTAATGTTTTATGGTACTGACCAATGAAAAGTTCTGCTTTTTTGTTTTGACCAGTTATGAGCTTGGGAGAAGGTGCGATAAAGGAGGATGGGCAACTAATAGCTAAATTATTTGGATCTGTGGGTAAGATATGGGAAGCTGAAGAGAAGTTGTTTGATGCAATCACTGGTTTGAGGTATGTTACATGTTTGCAAGAGTTGAGTCCCACAGTTTTACAAGACTCAAAAATTTCTTCTTTCTATTGCCACTTCCTAATTATGTTGGAGCCTGCTTCTGTTGAGGATACAAGATAAAATGATAGGATATTATCAGAATATGTATTTTGTTAGGATATTAGAATACTACTGGAGTATTTATGACCGTTCATAAATAAAGTGTGAGATCTCACATTGATCGTTCTAAATGGGAATGGAGTGTGAGATTCCACACCGATTAGAGAGAGGAATGAGTGTCAGCGAAGATGCTGGGGCCTGAAGAGGGTAGATTGTGAGATCCCACCTCGGTTAGAGAGGGAAACGAAACATTCTTTATAAGGATGTGGAAACCTCTCCCAAGCATACGCGACTTAAAAGTCTTGAGAGGAACCCCGAAAGGAAAAGCCCAAAAAGAACAATATCTACTAGCGGTGGCTTGGGCTATTACATAAAGGGTTTAGAGACGAGCATATTTTTTATATAGCCTCGGTAGAGAACTAAGCCTCTCAGACGGTGGGTAACCCCTTGTAATTTTCTTTCTAACGCATCCTTCCAGTTTTAGGGCCCTGGCATTCTTCAACTAATAGATACCATTATCTGTAAATGGAAATGTGCAGCGGTAGTGGGCCAGCGTACATATTCTTAGCAATAGAAGCTTTGGCTGACGGAGGAGTTGCTGCAGGTCTACCACGAGAACTTGCACTGGGTCTAGCTTCTCAAACTGTGAGTCAAATTCATCTGATATACCTGTCTAAAATAATTCATGCTGTGGGTCATATTGACATAACTCATCTGTAATTAATGTATTGCTTCTTTATCTTGCCATTTTATTTTACAATAAGAGAAAAGATCGAACTTGGCTTTTGATTTGAAGGACAAAAAGCTCAAAAGTAAACGGTCTTAGAAGAAAATTAAATTTTCAAACGAGGATGTTCTACCATACGTATAATTAAATAACTGAAGAAGCAGACGCTTCTATGATATATAATGCTTGCATATTCCAACCTTATTATCTGATAGTAGTCATACAGAACATGAGGATGATAAAAGTTATCACGAGATCCCATCAGTTGGAGAAGGGAACAAAACATTTTTTATAAGGGTGTGGAAAACTCTCCCTCAGGCGTTTTAAAGTCGTGAGACTGACAGCGATACATAAGGGTCTAAAGCGGACAATATCTGCTTGCAGTGGGCTTTGGCTGTTACAAATGGTATCAGAGGCAAACACCGGACGATATGCCAGCGAGGACGTTGGCCTCCCAAGGTGAATGGATTGTGAAATCCAATTTCCATTGGAGAGGGGAACGAAGCATTCCTTATAAGGGTGTGGAAACCTCTCCCTAGTTGTGAGGTTGACGGAGATACGTAACGGGCTAAAGTGGAGAATATCTGCTAGCAATGGACTTAAGTTGTTACAAATGGTATTAGAGCCAAACACCAGATGGTATGTCAGCGAGGACGTTAGACTCCCAAGGGGGTGGATTGTGAGATCCCATATGGATTGGAGAGGGGAACGAAACATTCCTTATAAGGGTGTGGAAACCTCTCCCTAGCTGTGAGGCTGACGACTACATAACAAACTAAAGTAGACAATATCTGCTAGCAGTGAACTTTGGCTATTCCAAATGGTATTAGAGCCAGACACCGGATAGTGTGCTTGCAAGGATGTTGGGCCCCCAAGGGGGGTAGATTTTGAGATCCCACATTAGTTGGAGAAGGGAACAAAGCATTTCTTATAAGGGTGTGGATACCTCTCCCTAATTGTGAGACTGACAACGATACGTAACGGGCTAAAGCACACAATATCTACTAGCAGTGGCTTCAGATGCTACAGTAAAATATTTCTTATTTCTTAAACTAAACAGCCATGAACTCATGATCTTCCACTATGTAGGCAGGTCATCCATCTTAGCTCCTAACTGCACTAGTTCCATATGACTTCCCATCAAGTGAAACATGTTAAGCGTAGTTATTTGGTTGTGAACCAATTGAAATGAACTGATCCAGAATTTGTTTACAGGTGCTAGGTGCAGCCACCATGATCACCAAAACTGGGAAGCATCCAGGACAGCTGAAGGATGATGTAACATCACCTGGAGGGACAACAATTGCAGGGATTCATGAACTAGAGAAGGGAAGACTCCGTGGCATATTCATGAACGCTGTGGTCGCTGCTGCGAAGCGTAGCCAAGAGCTTTCCGAAAAGTAGTTGCCAATATTGAACTTGCTAGTAAGGTTAGAGTTGATTTTATTTTGTTTCTAAGTCTTGAAGAGGTGATGAAATAAAGTAAACTTGCACCCTTTCCTTTGCAATATGAATGAAGAACCAAATCCATCACTCTTTTAGGCTTATTGTTTTTAGCTTAGATTCCTCCCTCCCTTCCCCCCTTTTGAAAGTTTTTGAATCTGTGCTTGGACTAGTTGTACAACCATTAGGGTGAGCCTTGAAATGTCGAAAAATCGAAC

mRNA sequence

TTGCCAAAATGGATCCTCCTTCTTCAAAACAAACGGGATTTCGCGGTTGAGCCGTTAGATGTGATAGCGTAGCCAGTAGTAGCCGGAGGAGAGCTTTGGATCGGAATCAATGGAGTTCCTTCCGATTTCACTTGATGCTTACAGATTAGGGTTCATCGGAGCTGGAAAAATGGCGGAGAGTCTAGCAAGGGGAATCGTCCAATCTGGTCTCTTACCTCCTTCTCGAATCTCCACGGCTCTCTACCCCACTCCTGGCCCCCCCATTGATTTCGAATCTTTCGGTGTTCGAGTCCTCCCCAAGAACGAAAATGTGGTTGAAGAAAGTGATGTGGTCATCTTCTCTGTAAAGCCTCAAATCGGTACGGCGCTTGTTCATCTCATCTACTCTTGTCCTGTTTTCCCCATTTTTAAGAATGTGGTGTTGAAGCTGAGGCCTCTACTGGGGAAGAAGCTTTTGGTTTCGATTGCTGCTGGGATAAAATTGAAGGATCTGCAGGATTGGGCAGGTCATAACCGATTCATTAGAGTTATGCCAAATACTCCAGCAGCTGTTGGAGAGGCTGCATCAGTTATGAGCTTGGGAGAAGGTGCGATAAAGGAGGATGGGCAACTAATAGCTAAATTATTTGGATCTGTGGGTAAGATATGGGAAGCTGAAGAGAAGTTGTTTGATGCAATCACTGGTTTGAGCGGTAGTGGGCCAGCGTACATATTCTTAGCAATAGAAGCTTTGGCTGACGGAGGAGTTGCTGCAGGTCTACCACGAGAACTTGCACTGGGTCTAGCTTCTCAAACTGTGCTAGGTGCAGCCACCATGATCACCAAAACTGGGAAGCATCCAGGACAGCTGAAGGATGATGTAACATCACCTGGAGGGACAACAATTGCAGGGATTCATGAACTAGAGAAGGGAAGACTCCGTGGCATATTCATGAACGCTGTGGTCGCTGCTGCGAAGCGTAGCCAAGAGCTTTCCGAAAAGTAGTTGCCAATATTGAACTTGCTAGTAAGGTTAGAGTTGATTTTATTTTGTTTCTAAGTCTTGAAGAGGTGATGAAATAAAGTAAACTTGCACCCTTTCCTTTGCAATATGAATGAAGAACCAAATCCATCACTCTTTTAGGCTTATTGTTTTTAGCTTAGATTCCTCCCTCCCTTCCCCCCTTTTGAAAGTTTTTGAATCTGTGCTTGGACTAGTTGTACAACCATTAGGGTGAGCCTTGAAATGTCGAAAAATCGAAC

Coding sequence (CDS)

ATGGAGTTCCTTCCGATTTCACTTGATGCTTACAGATTAGGGTTCATCGGAGCTGGAAAAATGGCGGAGAGTCTAGCAAGGGGAATCGTCCAATCTGGTCTCTTACCTCCTTCTCGAATCTCCACGGCTCTCTACCCCACTCCTGGCCCCCCCATTGATTTCGAATCTTTCGGTGTTCGAGTCCTCCCCAAGAACGAAAATGTGGTTGAAGAAAGTGATGTGGTCATCTTCTCTGTAAAGCCTCAAATCGGTACGGCGCTTGTTCATCTCATCTACTCTTGTCCTGTTTTCCCCATTTTTAAGAATGTGGTGTTGAAGCTGAGGCCTCTACTGGGGAAGAAGCTTTTGGTTTCGATTGCTGCTGGGATAAAATTGAAGGATCTGCAGGATTGGGCAGGTCATAACCGATTCATTAGAGTTATGCCAAATACTCCAGCAGCTGTTGGAGAGGCTGCATCAGTTATGAGCTTGGGAGAAGGTGCGATAAAGGAGGATGGGCAACTAATAGCTAAATTATTTGGATCTGTGGGTAAGATATGGGAAGCTGAAGAGAAGTTGTTTGATGCAATCACTGGTTTGAGCGGTAGTGGGCCAGCGTACATATTCTTAGCAATAGAAGCTTTGGCTGACGGAGGAGTTGCTGCAGGTCTACCACGAGAACTTGCACTGGGTCTAGCTTCTCAAACTGTGCTAGGTGCAGCCACCATGATCACCAAAACTGGGAAGCATCCAGGACAGCTGAAGGATGATGTAACATCACCTGGAGGGACAACAATTGCAGGGATTCATGAACTAGAGAAGGGAAGACTCCGTGGCATATTCATGAACGCTGTGGTCGCTGCTGCGAAGCGTAGCCAAGAGCTTTCCGAAAAGTAG

Protein sequence

MEFLPISLDAYRLGFIGAGKMAESLARGIVQSGLLPPSRISTALYPTPGPPIDFESFGVRVLPKNENVVEESDVVIFSVKPQIGTALVHLIYSCPVFPIFKNVVLKLRPLLGKKLLVSIAAGIKLKDLQDWAGHNRFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQLIAKLFGSVGKIWEAEEKLFDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAATMITKTGKHPGQLKDDVTSPGGTTIAGIHELEKGRLRGIFMNAVVAAAKRSQELSEK
Homology
BLAST of CmoCh03G001720 vs. ExPASy Swiss-Prot
Match: O04016 (Pyrroline-5-carboxylate reductase OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc23110 PE=2 SV=2)

HSP 1 Score: 387.1 bits (993), Expect = 1.7e-106
Identity = 207/290 (71.38%), Postives = 238/290 (82.07%), Query Frame = 0

Query: 3   FLPISLDAYRLGFIGAGKMAESLARGIVQSGLLPPSRISTALYPTPGPPIDFESFGVRVL 62
           F+PI  D Y+LGFIGAGKMAES+ARG+V+SG+LP SRI TA   +      FESFGV+VL
Sbjct: 6   FVPIPTDTYKLGFIGAGKMAESIARGVVKSGVLPASRIRTAHLGSARRDA-FESFGVKVL 65

Query: 63  PKNENVVEESDVVIFSVKPQIGTALVHLIYSCPVFPIFKNVVLKLRPLLG-KKLLVSIAA 122
            +N+ VVE+SDV+IFSVKPQ                I K VVL+LRPLL  K+LLVSI A
Sbjct: 66  DRNDQVVEDSDVIIFSVKPQ----------------IVKEVVLQLRPLLSEKQLLVSIVA 125

Query: 123 GIKLKDLQDWAGHNRFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQLIAKLFGSVGKIWE 182
           G+KLK+L+DWAGH+RFIRVMPNTPAAVGEAASVMSLG  A  EDG+LI KLFG++GKIW+
Sbjct: 126 GVKLKELEDWAGHSRFIRVMPNTPAAVGEAASVMSLGATATGEDGELITKLFGAIGKIWK 185

Query: 183 AEEKLFDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAATMITKTG 242
           A+EKLFDA+TGLSGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAA+M+ + G
Sbjct: 186 ADEKLFDAVTGLSGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAASMVARGG 245

Query: 243 KHPGQLKDDVTSPGGTTIAGIHELEKGRLRGIFMNAVVAAAKRSQELSEK 292
           KHPGQLKDDV S GGTTIAGIHELEKG  RG  MNAVV+A KRSQE+ ++
Sbjct: 246 KHPGQLKDDVASAGGTTIAGIHELEKGGFRGTLMNAVVSATKRSQEIFKR 278

BLAST of CmoCh03G001720 vs. ExPASy Swiss-Prot
Match: P17817 (Pyrroline-5-carboxylate reductase OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 3.0e-106
Identity = 206/290 (71.03%), Postives = 237/290 (81.72%), Query Frame = 0

Query: 1   MEFLPISLDAYRLGFIGAGKMAESLARGIVQSGLLPPSRISTALYPTPGPPIDFESFGVR 60
           ME  PI  ++Y LGFIGAGKMAES+ARG V+SG+LPPSRI TA++        FESFGV 
Sbjct: 1   MEIFPIPAESYTLGFIGAGKMAESIARGAVRSGVLPPSRIRTAVHFNLARRGAFESFGVT 60

Query: 61  VLPKNENVVEESDVVIFSVKPQIGTALVHLIYSCPVFPIFKNVVLKLRPLLGK-KLLVSI 120
           VLP N++VV ESDVV+ SVKPQ                + K+VV KL PLL K KLLVS+
Sbjct: 61  VLPSNDDVVRESDVVVLSVKPQ----------------LVKDVVSKLTPLLTKHKLLVSV 120

Query: 121 AAGIKLKDLQDWAGHNRFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQLIAKLFGSVGKI 180
           AAG KLKDLQ+WAG++RFIRVMPNTPAAVG+AASVMSLG  A +EDG +IA+LFGS+GKI
Sbjct: 121 AAGTKLKDLQEWAGNDRFIRVMPNTPAAVGQAASVMSLGGSATEEDGNIIAQLFGSIGKI 180

Query: 181 WEAEEKLFDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAATMITK 240
           W+AEEK FDAITGLSGSGPAY++LAIEALADGGVAAGLPR+L+L LASQTVLGAA+M+++
Sbjct: 181 WKAEEKYFDAITGLSGSGPAYVYLAIEALADGGVAAGLPRDLSLSLASQTVLGAASMVSQ 240

Query: 241 TGKHPGQLKDDVTSPGGTTIAGIHELEKGRLRGIFMNAVVAAAKRSQELS 290
           TGKHPGQLKDDVTSPGGTTI GIHELE G  RG  MNAVVAAAKRS+ELS
Sbjct: 241 TGKHPGQLKDDVTSPGGTTITGIHELENGGFRGTLMNAVVAAAKRSRELS 274

BLAST of CmoCh03G001720 vs. ExPASy Swiss-Prot
Match: Q04708 (Pyrroline-5-carboxylate reductase OS=Pisum sativum OX=3888 GN=PROC PE=2 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 2.1e-104
Identity = 205/290 (70.69%), Postives = 235/290 (81.03%), Query Frame = 0

Query: 1   MEFLPISLDAYRLGFIGAGKMAESLARGIVQSGLLPPSRISTALYPTPGPPIDFESFGVR 60
           ME LPI  D+Y LGFIGAGKMAES+A+G  +SG+LP SRI TA +  P     FES G+ 
Sbjct: 1   MEILPILSDSYTLGFIGAGKMAESIAKGASRSGVLPSSRIVTA-HSNPSRRAAFESIGIT 60

Query: 61  VLPKNENVVEESDVVIFSVKPQIGTALVHLIYSCPVFPIFKNVVLKLRPLLGK-KLLVSI 120
           VL  N++VV  S+VV+FSVKPQ                + K+VVLKL+PLL K KLLVS+
Sbjct: 61  VLSSNDDVVRASNVVVFSVKPQ----------------LVKDVVLKLKPLLTKDKLLVSV 120

Query: 121 AAGIKLKDLQDWAGHNRFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQLIAKLFGSVGKI 180
           AAGIKLKDLQ+WAGH RFIRVMPNTPAAVG+AASVMSLG  A +ED  LI++LFGS+GKI
Sbjct: 121 AAGIKLKDLQEWAGHERFIRVMPNTPAAVGQAASVMSLGGAATEEDANLISQLFGSIGKI 180

Query: 181 WEAEEKLFDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAATMITK 240
           W+A++K FDAITGLSGSGPAYI+LAIEALADGGVAAGLPR+LAL LASQTVLGAA+M T 
Sbjct: 181 WKADDKFFDAITGLSGSGPAYIYLAIEALADGGVAAGLPRDLALSLASQTVLGAASMATL 240

Query: 241 TGKHPGQLKDDVTSPGGTTIAGIHELEKGRLRGIFMNAVVAAAKRSQELS 290
           +GKHPGQLKDDVTSPGGTTIAG+HELEKG  RG  MNAVVAAAKRS+ELS
Sbjct: 241 SGKHPGQLKDDVTSPGGTTIAGVHELEKGGFRGTLMNAVVAAAKRSRELS 273

BLAST of CmoCh03G001720 vs. ExPASy Swiss-Prot
Match: P54904 (Pyrroline-5-carboxylate reductase OS=Arabidopsis thaliana OX=3702 GN=PROC1 PE=2 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 8.1e-104
Identity = 201/291 (69.07%), Postives = 235/291 (80.76%), Query Frame = 0

Query: 1   MEFLPISLDAYRLGFIGAGKMAESLARGIVQSGLLPPSRISTALYPTPGPPIDFESFGVR 60
           ME LPI  +++++GFIGAGKMAES+ARG+V SG+LPP+RI TA++        FESFGV 
Sbjct: 1   MEILPIPAESFKVGFIGAGKMAESIARGVVASGVLPPNRICTAVHSNLNRRDVFESFGVN 60

Query: 61  VLPKNENVVEESDVVIFSVKPQIGTALVHLIYSCPVFPIFKNVVLKLRPLLGK-KLLVSI 120
           V   +E VV+ESDVVIFSVKPQ                + K  V +L+  L K K+LVS+
Sbjct: 61  VFSTSEEVVKESDVVIFSVKPQ----------------VVKKAVTELKSKLSKNKILVSV 120

Query: 121 AAGIKLKDLQDWAGHNRFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQLIAKLFGSVGKI 180
           AAGIKL DLQ+W+G +RFIRVMPNTPAAVGEAASVMSLG GA +EDG ++A LFG+VGKI
Sbjct: 121 AAGIKLNDLQEWSGQDRFIRVMPNTPAAVGEAASVMSLGTGATEEDGAIVAMLFGAVGKI 180

Query: 181 WEAEEKLFDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAATMITK 240
            +A+EK+FDA+TGLSGSGPAYIFLAIEALADGGVAAGLPRELAL LASQTVLGAATM++K
Sbjct: 181 LKADEKMFDAVTGLSGSGPAYIFLAIEALADGGVAAGLPRELALSLASQTVLGAATMVSK 240

Query: 241 TGKHPGQLKDDVTSPGGTTIAGIHELEKGRLRGIFMNAVVAAAKRSQELSE 291
           TGKHPG LKDDVTSPGGTTIAG+HELEKG  R   MNAVVAAAKRS+ELS+
Sbjct: 241 TGKHPGVLKDDVTSPGGTTIAGVHELEKGSFRATLMNAVVAAAKRSRELSQ 275

BLAST of CmoCh03G001720 vs. ExPASy Swiss-Prot
Match: Q9HH99 (Pyrroline-5-carboxylate reductase OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) OX=188937 GN=proC PE=3 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 1.8e-55
Identity = 127/285 (44.56%), Postives = 176/285 (61.75%), Query Frame = 0

Query: 8   LDAYRLGFIGAGKMAESLARGIVQSGLLPPSRISTALYPTPGPPIDFESFGVRVLPKNEN 67
           ++  ++GFIGAGKM  +L +G +++G++ P  I  +    P         G+RV   N  
Sbjct: 1   MENQKIGFIGAGKMGSALMQGTIKAGIVTPENIGASDVYEPFLKDLQAKLGIRVSTDNAV 60

Query: 68  VVEESDVVIFSVKPQIGTALVHLIYSCPVFPIFKNVVLKLRPLLGKKLLVSIAAGIKLKD 127
           +V ESD++I +VKPQ  ++         V    KN       +  +KL++SIAAG+ L  
Sbjct: 61  IVRESDILILAVKPQTLSS---------VLSNLKN------EITSEKLVISIAAGVPLST 120

Query: 128 LQD-WAGHNRFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQLIAKLFGSVGKIWEAEEKL 187
            +D      R +RVMPN  A V EAAS ++ G+ A  ED +   ++F +VG   +  E L
Sbjct: 121 YEDALLEGTRVVRVMPNIAATVSEAASGIAPGKNATPEDLKAALEIFSAVGTAVQVPESL 180

Query: 188 FDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAATMITKTGKHPGQ 247
            DA+TGLSGSGPA+IF  IEA+ADG V  G+ R+ AL LA+QTVLGAA M  +TG HPG+
Sbjct: 181 MDAVTGLSGSGPAFIFPVIEAMADGAVLEGMDRKSALTLAAQTVLGAAKMALETGMHPGE 240

Query: 248 LKDDVTSPGGTTIAGIHELEKGRLRGIFMNAVVAAAKRSQELSEK 292
           LKD VTSP GTTI GIH LE+  +R  FMNAV+ A++RS+EL +K
Sbjct: 241 LKDMVTSPAGTTIQGIHSLEEAGIRAAFMNAVIRASERSKELGKK 270

BLAST of CmoCh03G001720 vs. ExPASy TrEMBL
Match: A0A6J1F384 (Pyrroline-5-carboxylate reductase OS=Cucurbita moschata OX=3662 GN=LOC111439386 PE=3 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 7.0e-143
Identity = 274/291 (94.16%), Postives = 274/291 (94.16%), Query Frame = 0

Query: 1   MEFLPISLDAYRLGFIGAGKMAESLARGIVQSGLLPPSRISTALYPTPGPPIDFESFGVR 60
           MEFLPISLDAYRLGFIGAGKMAESLARGIVQSGLLPPSRISTALYPTPGPPIDFESFGVR
Sbjct: 1   MEFLPISLDAYRLGFIGAGKMAESLARGIVQSGLLPPSRISTALYPTPGPPIDFESFGVR 60

Query: 61  VLPKNENVVEESDVVIFSVKPQIGTALVHLIYSCPVFPIFKNVVLKLRPLLGKKLLVSIA 120
           VLPKNENVVEESDVVIFSVKPQ                I KNVVLKLRPLLGKKLLVSIA
Sbjct: 61  VLPKNENVVEESDVVIFSVKPQ----------------IVKNVVLKLRPLLGKKLLVSIA 120

Query: 121 AGIKLKDLQDWAGHNRFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQLIAKLFGSVGKIW 180
           AGIKLKDLQDWAGHNRFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQLIAKLFGSVGKIW
Sbjct: 121 AGIKLKDLQDWAGHNRFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQLIAKLFGSVGKIW 180

Query: 181 EAEEKLFDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAATMITKT 240
           EAEEKLFDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAATMITKT
Sbjct: 181 EAEEKLFDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAATMITKT 240

Query: 241 GKHPGQLKDDVTSPGGTTIAGIHELEKGRLRGIFMNAVVAAAKRSQELSEK 292
           GKHPGQLKDDVTSPGGTTIAGIHELEKGRLRGIFMNAVVAAAKRSQELSEK
Sbjct: 241 GKHPGQLKDDVTSPGGTTIAGIHELEKGRLRGIFMNAVVAAAKRSQELSEK 275

BLAST of CmoCh03G001720 vs. ExPASy TrEMBL
Match: A0A6J1HS08 (Pyrroline-5-carboxylate reductase OS=Cucurbita maxima OX=3661 GN=LOC111467254 PE=3 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 2.7e-142
Identity = 273/291 (93.81%), Postives = 274/291 (94.16%), Query Frame = 0

Query: 1   MEFLPISLDAYRLGFIGAGKMAESLARGIVQSGLLPPSRISTALYPTPGPPIDFESFGVR 60
           MEFLPISLDAYRLGFIGAGKMAESLARGIVQSGLLPPSRISTALYPTPGPPIDFESFGVR
Sbjct: 1   MEFLPISLDAYRLGFIGAGKMAESLARGIVQSGLLPPSRISTALYPTPGPPIDFESFGVR 60

Query: 61  VLPKNENVVEESDVVIFSVKPQIGTALVHLIYSCPVFPIFKNVVLKLRPLLGKKLLVSIA 120
           VLPKNENVVEESDVVIFSVKPQ                I KNVVLKLRPLLGKKLLVSIA
Sbjct: 61  VLPKNENVVEESDVVIFSVKPQ----------------IVKNVVLKLRPLLGKKLLVSIA 120

Query: 121 AGIKLKDLQDWAGHNRFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQLIAKLFGSVGKIW 180
           AGIKLKDLQDWAGH+RFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQLIAKLFGSVGKIW
Sbjct: 121 AGIKLKDLQDWAGHSRFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQLIAKLFGSVGKIW 180

Query: 181 EAEEKLFDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAATMITKT 240
           EAEEKLFDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAATMITKT
Sbjct: 181 EAEEKLFDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAATMITKT 240

Query: 241 GKHPGQLKDDVTSPGGTTIAGIHELEKGRLRGIFMNAVVAAAKRSQELSEK 292
           GKHPGQLKDDVTSPGGTTIAGIHELEKGRLRGIFMNAVVAAAKRSQELSEK
Sbjct: 241 GKHPGQLKDDVTSPGGTTIAGIHELEKGRLRGIFMNAVVAAAKRSQELSEK 275

BLAST of CmoCh03G001720 vs. ExPASy TrEMBL
Match: A0A5D3DFQ4 (Pyrroline-5-carboxylate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold523G00130 PE=3 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 2.2e-128
Identity = 247/292 (84.59%), Postives = 262/292 (89.73%), Query Frame = 0

Query: 1   MEFLPISLDAYRLGFIGAGKMAESLARGIVQSGLLPPSRISTALYPTPGPPIDFESFGVR 60
           ME LPIS+DAYRLGFIGAGKMAES+A+GIVQSGLLPPSRISTA++  P   I FESFGVR
Sbjct: 1   MEVLPISIDAYRLGFIGAGKMAESIAKGIVQSGLLPPSRISTAVHSNPSRRIAFESFGVR 60

Query: 61  VLPKNENVVEESDVVIFSVKPQIGTALVHLIYSCPVFPIFKNVVLKLRPLL-GKKLLVSI 120
           VLPKN+NVVEESDVVI SVKPQ                + KNVVLKLRPLL GKKLLVS+
Sbjct: 61  VLPKNDNVVEESDVVILSVKPQ----------------VVKNVVLKLRPLLSGKKLLVSV 120

Query: 121 AAGIKLKDLQDWAGHNRFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQLIAKLFGSVGKI 180
           AAG+KLKDLQDWAGHNRF+RVMPNTPAAVGEAASVMSLGEGAIKEDGQL+AKLFGSVGKI
Sbjct: 121 AAGVKLKDLQDWAGHNRFVRVMPNTPAAVGEAASVMSLGEGAIKEDGQLVAKLFGSVGKI 180

Query: 181 WEAEEKLFDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAATMITK 240
           WEAEEK FDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELA+GLASQTVLGAA+M+TK
Sbjct: 181 WEAEEKYFDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTK 240

Query: 241 TGKHPGQLKDDVTSPGGTTIAGIHELEKGRLRGIFMNAVVAAAKRSQELSEK 292
           TGKHPGQLKDDVTSPGGTTIAGIHELEKG LRG+FMNAVVAAAKR QELS K
Sbjct: 241 TGKHPGQLKDDVTSPGGTTIAGIHELEKGGLRGMFMNAVVAAAKRGQELSPK 276

BLAST of CmoCh03G001720 vs. ExPASy TrEMBL
Match: A0A0A0KYH5 (Pyrroline-5-carboxylate reductase OS=Cucumis sativus OX=3659 GN=Csa_4G354630 PE=3 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 2.2e-128
Identity = 247/292 (84.59%), Postives = 262/292 (89.73%), Query Frame = 0

Query: 1   MEFLPISLDAYRLGFIGAGKMAESLARGIVQSGLLPPSRISTALYPTPGPPIDFESFGVR 60
           ME LPIS+DAYRLGFIGAGKMAES+A+GIVQSGLLPPSRISTA++  P   I FESFGVR
Sbjct: 1   MEVLPISIDAYRLGFIGAGKMAESIAKGIVQSGLLPPSRISTAVHSNPSRRIAFESFGVR 60

Query: 61  VLPKNENVVEESDVVIFSVKPQIGTALVHLIYSCPVFPIFKNVVLKLRPLL-GKKLLVSI 120
           VLPKN+NVVEESDVVI SVKPQ                + KNVVLKLRPLL GKKLLVS+
Sbjct: 61  VLPKNDNVVEESDVVILSVKPQ----------------VVKNVVLKLRPLLSGKKLLVSV 120

Query: 121 AAGIKLKDLQDWAGHNRFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQLIAKLFGSVGKI 180
           AAG+KLKDLQDWAGHNRFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQL+AKLFGSVGKI
Sbjct: 121 AAGVKLKDLQDWAGHNRFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQLVAKLFGSVGKI 180

Query: 181 WEAEEKLFDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAATMITK 240
           WEAEEK FDA+TGLSGSGPAYIFLAIEALADGGVAAGLPRELA+GLASQTVLGAA+M+TK
Sbjct: 181 WEAEEKYFDAVTGLSGSGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTK 240

Query: 241 TGKHPGQLKDDVTSPGGTTIAGIHELEKGRLRGIFMNAVVAAAKRSQELSEK 292
           TGKHPGQLKDDVTSPGGTTIAGIHELEKG LRG+FMNAVVAAAKR QELS K
Sbjct: 241 TGKHPGQLKDDVTSPGGTTIAGIHELEKGGLRGMFMNAVVAAAKRGQELSPK 276

BLAST of CmoCh03G001720 vs. ExPASy TrEMBL
Match: A0A1S3C257 (Pyrroline-5-carboxylate reductase OS=Cucumis melo OX=3656 GN=LOC103495803 PE=3 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 2.2e-128
Identity = 247/292 (84.59%), Postives = 262/292 (89.73%), Query Frame = 0

Query: 1   MEFLPISLDAYRLGFIGAGKMAESLARGIVQSGLLPPSRISTALYPTPGPPIDFESFGVR 60
           ME LPIS+DAYRLGFIGAGKMAES+A+GIVQSGLLPPSRISTA++  P   I FESFGVR
Sbjct: 1   MEVLPISIDAYRLGFIGAGKMAESIAKGIVQSGLLPPSRISTAVHSNPSRRIAFESFGVR 60

Query: 61  VLPKNENVVEESDVVIFSVKPQIGTALVHLIYSCPVFPIFKNVVLKLRPLL-GKKLLVSI 120
           VLPKN+NVVEESDVVI SVKPQ                + KNVVLKLRPLL GKKLLVS+
Sbjct: 61  VLPKNDNVVEESDVVILSVKPQ----------------VVKNVVLKLRPLLSGKKLLVSV 120

Query: 121 AAGIKLKDLQDWAGHNRFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQLIAKLFGSVGKI 180
           AAG+KLKDLQDWAGHNRF+RVMPNTPAAVGEAASVMSLGEGAIKEDGQL+AKLFGSVGKI
Sbjct: 121 AAGVKLKDLQDWAGHNRFVRVMPNTPAAVGEAASVMSLGEGAIKEDGQLVAKLFGSVGKI 180

Query: 181 WEAEEKLFDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAATMITK 240
           WEAEEK FDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELA+GLASQTVLGAA+M+TK
Sbjct: 181 WEAEEKYFDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTK 240

Query: 241 TGKHPGQLKDDVTSPGGTTIAGIHELEKGRLRGIFMNAVVAAAKRSQELSEK 292
           TGKHPGQLKDDVTSPGGTTIAGIHELEKG LRG+FMNAVVAAAKR QELS K
Sbjct: 241 TGKHPGQLKDDVTSPGGTTIAGIHELEKGGLRGMFMNAVVAAAKRGQELSPK 276

BLAST of CmoCh03G001720 vs. NCBI nr
Match: XP_022932845.1 (pyrroline-5-carboxylate reductase-like [Cucurbita moschata] >XP_023543593.1 pyrroline-5-carboxylate reductase-like [Cucurbita pepo subsp. pepo] >KAG6603328.1 Pyrroline-5-carboxylate reductase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033601.1 hypothetical protein SDJN02_03325 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 516.5 bits (1329), Expect = 1.5e-142
Identity = 274/291 (94.16%), Postives = 274/291 (94.16%), Query Frame = 0

Query: 1   MEFLPISLDAYRLGFIGAGKMAESLARGIVQSGLLPPSRISTALYPTPGPPIDFESFGVR 60
           MEFLPISLDAYRLGFIGAGKMAESLARGIVQSGLLPPSRISTALYPTPGPPIDFESFGVR
Sbjct: 1   MEFLPISLDAYRLGFIGAGKMAESLARGIVQSGLLPPSRISTALYPTPGPPIDFESFGVR 60

Query: 61  VLPKNENVVEESDVVIFSVKPQIGTALVHLIYSCPVFPIFKNVVLKLRPLLGKKLLVSIA 120
           VLPKNENVVEESDVVIFSVKPQ                I KNVVLKLRPLLGKKLLVSIA
Sbjct: 61  VLPKNENVVEESDVVIFSVKPQ----------------IVKNVVLKLRPLLGKKLLVSIA 120

Query: 121 AGIKLKDLQDWAGHNRFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQLIAKLFGSVGKIW 180
           AGIKLKDLQDWAGHNRFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQLIAKLFGSVGKIW
Sbjct: 121 AGIKLKDLQDWAGHNRFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQLIAKLFGSVGKIW 180

Query: 181 EAEEKLFDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAATMITKT 240
           EAEEKLFDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAATMITKT
Sbjct: 181 EAEEKLFDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAATMITKT 240

Query: 241 GKHPGQLKDDVTSPGGTTIAGIHELEKGRLRGIFMNAVVAAAKRSQELSEK 292
           GKHPGQLKDDVTSPGGTTIAGIHELEKGRLRGIFMNAVVAAAKRSQELSEK
Sbjct: 241 GKHPGQLKDDVTSPGGTTIAGIHELEKGRLRGIFMNAVVAAAKRSQELSEK 275

BLAST of CmoCh03G001720 vs. NCBI nr
Match: XP_022967877.1 (pyrroline-5-carboxylate reductase-like [Cucurbita maxima])

HSP 1 Score: 514.6 bits (1324), Expect = 5.5e-142
Identity = 273/291 (93.81%), Postives = 274/291 (94.16%), Query Frame = 0

Query: 1   MEFLPISLDAYRLGFIGAGKMAESLARGIVQSGLLPPSRISTALYPTPGPPIDFESFGVR 60
           MEFLPISLDAYRLGFIGAGKMAESLARGIVQSGLLPPSRISTALYPTPGPPIDFESFGVR
Sbjct: 1   MEFLPISLDAYRLGFIGAGKMAESLARGIVQSGLLPPSRISTALYPTPGPPIDFESFGVR 60

Query: 61  VLPKNENVVEESDVVIFSVKPQIGTALVHLIYSCPVFPIFKNVVLKLRPLLGKKLLVSIA 120
           VLPKNENVVEESDVVIFSVKPQ                I KNVVLKLRPLLGKKLLVSIA
Sbjct: 61  VLPKNENVVEESDVVIFSVKPQ----------------IVKNVVLKLRPLLGKKLLVSIA 120

Query: 121 AGIKLKDLQDWAGHNRFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQLIAKLFGSVGKIW 180
           AGIKLKDLQDWAGH+RFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQLIAKLFGSVGKIW
Sbjct: 121 AGIKLKDLQDWAGHSRFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQLIAKLFGSVGKIW 180

Query: 181 EAEEKLFDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAATMITKT 240
           EAEEKLFDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAATMITKT
Sbjct: 181 EAEEKLFDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAATMITKT 240

Query: 241 GKHPGQLKDDVTSPGGTTIAGIHELEKGRLRGIFMNAVVAAAKRSQELSEK 292
           GKHPGQLKDDVTSPGGTTIAGIHELEKGRLRGIFMNAVVAAAKRSQELSEK
Sbjct: 241 GKHPGQLKDDVTSPGGTTIAGIHELEKGRLRGIFMNAVVAAAKRSQELSEK 275

BLAST of CmoCh03G001720 vs. NCBI nr
Match: XP_004142647.1 (pyrroline-5-carboxylate reductase [Cucumis sativus] >KGN54523.1 hypothetical protein Csa_012959 [Cucumis sativus])

HSP 1 Score: 468.4 bits (1204), Expect = 4.5e-128
Identity = 247/292 (84.59%), Postives = 262/292 (89.73%), Query Frame = 0

Query: 1   MEFLPISLDAYRLGFIGAGKMAESLARGIVQSGLLPPSRISTALYPTPGPPIDFESFGVR 60
           ME LPIS+DAYRLGFIGAGKMAES+A+GIVQSGLLPPSRISTA++  P   I FESFGVR
Sbjct: 1   MEVLPISIDAYRLGFIGAGKMAESIAKGIVQSGLLPPSRISTAVHSNPSRRIAFESFGVR 60

Query: 61  VLPKNENVVEESDVVIFSVKPQIGTALVHLIYSCPVFPIFKNVVLKLRPLL-GKKLLVSI 120
           VLPKN+NVVEESDVVI SVKPQ                + KNVVLKLRPLL GKKLLVS+
Sbjct: 61  VLPKNDNVVEESDVVILSVKPQ----------------VVKNVVLKLRPLLSGKKLLVSV 120

Query: 121 AAGIKLKDLQDWAGHNRFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQLIAKLFGSVGKI 180
           AAG+KLKDLQDWAGHNRFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQL+AKLFGSVGKI
Sbjct: 121 AAGVKLKDLQDWAGHNRFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQLVAKLFGSVGKI 180

Query: 181 WEAEEKLFDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAATMITK 240
           WEAEEK FDA+TGLSGSGPAYIFLAIEALADGGVAAGLPRELA+GLASQTVLGAA+M+TK
Sbjct: 181 WEAEEKYFDAVTGLSGSGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTK 240

Query: 241 TGKHPGQLKDDVTSPGGTTIAGIHELEKGRLRGIFMNAVVAAAKRSQELSEK 292
           TGKHPGQLKDDVTSPGGTTIAGIHELEKG LRG+FMNAVVAAAKR QELS K
Sbjct: 241 TGKHPGQLKDDVTSPGGTTIAGIHELEKGGLRGMFMNAVVAAAKRGQELSPK 276

BLAST of CmoCh03G001720 vs. NCBI nr
Match: XP_008455688.1 (PREDICTED: pyrroline-5-carboxylate reductase [Cucumis melo] >KAA0062733.1 pyrroline-5-carboxylate reductase [Cucumis melo var. makuwa] >TYK22531.1 pyrroline-5-carboxylate reductase [Cucumis melo var. makuwa])

HSP 1 Score: 468.4 bits (1204), Expect = 4.5e-128
Identity = 247/292 (84.59%), Postives = 262/292 (89.73%), Query Frame = 0

Query: 1   MEFLPISLDAYRLGFIGAGKMAESLARGIVQSGLLPPSRISTALYPTPGPPIDFESFGVR 60
           ME LPIS+DAYRLGFIGAGKMAES+A+GIVQSGLLPPSRISTA++  P   I FESFGVR
Sbjct: 1   MEVLPISIDAYRLGFIGAGKMAESIAKGIVQSGLLPPSRISTAVHSNPSRRIAFESFGVR 60

Query: 61  VLPKNENVVEESDVVIFSVKPQIGTALVHLIYSCPVFPIFKNVVLKLRPLL-GKKLLVSI 120
           VLPKN+NVVEESDVVI SVKPQ                + KNVVLKLRPLL GKKLLVS+
Sbjct: 61  VLPKNDNVVEESDVVILSVKPQ----------------VVKNVVLKLRPLLSGKKLLVSV 120

Query: 121 AAGIKLKDLQDWAGHNRFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQLIAKLFGSVGKI 180
           AAG+KLKDLQDWAGHNRF+RVMPNTPAAVGEAASVMSLGEGAIKEDGQL+AKLFGSVGKI
Sbjct: 121 AAGVKLKDLQDWAGHNRFVRVMPNTPAAVGEAASVMSLGEGAIKEDGQLVAKLFGSVGKI 180

Query: 181 WEAEEKLFDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAATMITK 240
           WEAEEK FDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELA+GLASQTVLGAA+M+TK
Sbjct: 181 WEAEEKYFDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTK 240

Query: 241 TGKHPGQLKDDVTSPGGTTIAGIHELEKGRLRGIFMNAVVAAAKRSQELSEK 292
           TGKHPGQLKDDVTSPGGTTIAGIHELEKG LRG+FMNAVVAAAKR QELS K
Sbjct: 241 TGKHPGQLKDDVTSPGGTTIAGIHELEKGGLRGMFMNAVVAAAKRGQELSPK 276

BLAST of CmoCh03G001720 vs. NCBI nr
Match: XP_038881291.1 (pyrroline-5-carboxylate reductase isoform X1 [Benincasa hispida])

HSP 1 Score: 466.8 bits (1200), Expect = 1.3e-127
Identity = 246/292 (84.25%), Postives = 263/292 (90.07%), Query Frame = 0

Query: 1   MEFLPISLDAYRLGFIGAGKMAESLARGIVQSGLLPPSRISTALYPTPGPPIDFESFGVR 60
           ME LPIS+DAYRLGFIGAGKMAES+A+GIVQSGLLPPSRISTA++  P   I FESFGV+
Sbjct: 1   MEVLPISIDAYRLGFIGAGKMAESIAKGIVQSGLLPPSRISTAVHSNPSRRIAFESFGVQ 60

Query: 61  VLPKNENVVEESDVVIFSVKPQIGTALVHLIYSCPVFPIFKNVVLKLRPLL-GKKLLVSI 120
           VLPKN+NVVEESDVVI SVKPQ                + KNVVLKLRPLL GKKLLVS+
Sbjct: 61  VLPKNDNVVEESDVVILSVKPQ----------------VVKNVVLKLRPLLSGKKLLVSV 120

Query: 121 AAGIKLKDLQDWAGHNRFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQLIAKLFGSVGKI 180
           AAG+KLKDLQDWAGHNRFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQL+AKLFGSVGKI
Sbjct: 121 AAGVKLKDLQDWAGHNRFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQLVAKLFGSVGKI 180

Query: 181 WEAEEKLFDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAATMITK 240
           WEA+EK FDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELA+GLASQTVLGAA+M+TK
Sbjct: 181 WEADEKFFDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTK 240

Query: 241 TGKHPGQLKDDVTSPGGTTIAGIHELEKGRLRGIFMNAVVAAAKRSQELSEK 292
           TGKHPGQLKDDVTSPGGTTIAGIHELEKG LRG+FMNAVVAAAKRSQ+LS K
Sbjct: 241 TGKHPGQLKDDVTSPGGTTIAGIHELEKGGLRGMFMNAVVAAAKRSQDLSPK 276

BLAST of CmoCh03G001720 vs. TAIR 10
Match: AT5G14800.1 (pyrroline-5- carboxylate (P5C) reductase )

HSP 1 Score: 378.3 bits (970), Expect = 5.7e-105
Identity = 201/291 (69.07%), Postives = 235/291 (80.76%), Query Frame = 0

Query: 1   MEFLPISLDAYRLGFIGAGKMAESLARGIVQSGLLPPSRISTALYPTPGPPIDFESFGVR 60
           ME LPI  +++++GFIGAGKMAES+ARG+V SG+LPP+RI TA++        FESFGV 
Sbjct: 1   MEILPIPAESFKVGFIGAGKMAESIARGVVASGVLPPNRICTAVHSNLNRRDVFESFGVN 60

Query: 61  VLPKNENVVEESDVVIFSVKPQIGTALVHLIYSCPVFPIFKNVVLKLRPLLGK-KLLVSI 120
           V   +E VV+ESDVVIFSVKPQ                + K  V +L+  L K K+LVS+
Sbjct: 61  VFSTSEEVVKESDVVIFSVKPQ----------------VVKKAVTELKSKLSKNKILVSV 120

Query: 121 AAGIKLKDLQDWAGHNRFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQLIAKLFGSVGKI 180
           AAGIKL DLQ+W+G +RFIRVMPNTPAAVGEAASVMSLG GA +EDG ++A LFG+VGKI
Sbjct: 121 AAGIKLNDLQEWSGQDRFIRVMPNTPAAVGEAASVMSLGTGATEEDGAIVAMLFGAVGKI 180

Query: 181 WEAEEKLFDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAATMITK 240
            +A+EK+FDA+TGLSGSGPAYIFLAIEALADGGVAAGLPRELAL LASQTVLGAATM++K
Sbjct: 181 LKADEKMFDAVTGLSGSGPAYIFLAIEALADGGVAAGLPRELALSLASQTVLGAATMVSK 240

Query: 241 TGKHPGQLKDDVTSPGGTTIAGIHELEKGRLRGIFMNAVVAAAKRSQELSE 291
           TGKHPG LKDDVTSPGGTTIAG+HELEKG  R   MNAVVAAAKRS+ELS+
Sbjct: 241 TGKHPGVLKDDVTSPGGTTIAGVHELEKGSFRATLMNAVVAAAKRSRELSQ 275

BLAST of CmoCh03G001720 vs. TAIR 10
Match: AT5G14800.2 (pyrroline-5- carboxylate (P5C) reductase )

HSP 1 Score: 281.6 bits (719), Expect = 7.3e-76
Identity = 152/231 (65.80%), Postives = 181/231 (78.35%), Query Frame = 0

Query: 1   MEFLPISLDAYRLGFIGAGKMAESLARGIVQSGLLPPSRISTALYPTPGPPIDFESFGVR 60
           ME LPI  +++++GFIGAGKMAES+ARG+V SG+LPP+RI TA++        FESFGV 
Sbjct: 1   MEILPIPAESFKVGFIGAGKMAESIARGVVASGVLPPNRICTAVHSNLNRRDVFESFGVN 60

Query: 61  VLPKNENVVEESDVVIFSVKPQIGTALVHLIYSCPVFPIFKNVVLKLRPLLGK-KLLVSI 120
           V   +E VV+ESDVVIFSVKPQ                + K  V +L+  L K K+LVS+
Sbjct: 61  VFSTSEEVVKESDVVIFSVKPQ----------------VVKKAVTELKSKLSKNKILVSV 120

Query: 121 AAGIKLKDLQDWAGHNRFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQLIAKLFGSVGKI 180
           AAGIKL DLQ+W+G +RFIRVMPNTPAAVGEAASVMSLG GA +EDG ++A LFG+VGKI
Sbjct: 121 AAGIKLNDLQEWSGQDRFIRVMPNTPAAVGEAASVMSLGTGATEEDGAIVAMLFGAVGKI 180

Query: 181 WEAEEKLFDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTV 231
            +A+EK+FDA+TGLSGSGPAYIFLAIEALADGGVAAGLPRELAL LASQTV
Sbjct: 181 LKADEKMFDAVTGLSGSGPAYIFLAIEALADGGVAAGLPRELALSLASQTV 215

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O040161.7e-10671.38Pyrroline-5-carboxylate reductase OS=Actinidia chinensis var. chinensis OX=15908... [more]
P178173.0e-10671.03Pyrroline-5-carboxylate reductase OS=Glycine max OX=3847 PE=2 SV=1[more]
Q047082.1e-10470.69Pyrroline-5-carboxylate reductase OS=Pisum sativum OX=3888 GN=PROC PE=2 SV=1[more]
P549048.1e-10469.07Pyrroline-5-carboxylate reductase OS=Arabidopsis thaliana OX=3702 GN=PROC1 PE=2 ... [more]
Q9HH991.8e-5544.56Pyrroline-5-carboxylate reductase OS=Methanosarcina acetivorans (strain ATCC 353... [more]
Match NameE-valueIdentityDescription
A0A6J1F3847.0e-14394.16Pyrroline-5-carboxylate reductase OS=Cucurbita moschata OX=3662 GN=LOC111439386 ... [more]
A0A6J1HS082.7e-14293.81Pyrroline-5-carboxylate reductase OS=Cucurbita maxima OX=3661 GN=LOC111467254 PE... [more]
A0A5D3DFQ42.2e-12884.59Pyrroline-5-carboxylate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A0A0KYH52.2e-12884.59Pyrroline-5-carboxylate reductase OS=Cucumis sativus OX=3659 GN=Csa_4G354630 PE=... [more]
A0A1S3C2572.2e-12884.59Pyrroline-5-carboxylate reductase OS=Cucumis melo OX=3656 GN=LOC103495803 PE=3 S... [more]
Match NameE-valueIdentityDescription
XP_022932845.11.5e-14294.16pyrroline-5-carboxylate reductase-like [Cucurbita moschata] >XP_023543593.1 pyrr... [more]
XP_022967877.15.5e-14293.81pyrroline-5-carboxylate reductase-like [Cucurbita maxima][more]
XP_004142647.14.5e-12884.59pyrroline-5-carboxylate reductase [Cucumis sativus] >KGN54523.1 hypothetical pro... [more]
XP_008455688.14.5e-12884.59PREDICTED: pyrroline-5-carboxylate reductase [Cucumis melo] >KAA0062733.1 pyrrol... [more]
XP_038881291.11.3e-12784.25pyrroline-5-carboxylate reductase isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G14800.15.7e-10569.07pyrroline-5- carboxylate (P5C) reductase [more]
AT5G14800.27.3e-7665.80pyrroline-5- carboxylate (P5C) reductase [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 278..291
NoneNo IPR availableGENE3D1.10.3730.10coord: 187..291
e-value: 2.1E-44
score: 152.2
NoneNo IPR availableGENE3D3.40.50.720coord: 1..186
e-value: 9.8E-50
score: 170.5
NoneNo IPR availablePANTHERPTHR11645:SF0PYRROLINE-5-CARBOXYLATE REDUCTASE 2coord: 10..290
IPR028939Pyrroline-5-carboxylate reductase, catalytic, N-terminalPFAMPF03807F420_oxidoredcoord: 12..88
e-value: 1.0E-11
score: 45.3
IPR000304Pyrroline-5-carboxylate reductasePIRSFPIRSF000193P5CRcoord: 97..291
e-value: 5.2E-62
score: 207.1
coord: 10..93
e-value: 2.7E-13
score: 47.4
IPR000304Pyrroline-5-carboxylate reductaseTIGRFAMTIGR00112TIGR00112coord: 13..288
e-value: 8.9E-79
score: 262.8
IPR000304Pyrroline-5-carboxylate reductasePANTHERPTHR11645PYRROLINE-5-CARBOXYLATE REDUCTASEcoord: 10..290
IPR000304Pyrroline-5-carboxylate reductasePROSITEPS00521P5CRcoord: 244..266
IPR000304Pyrroline-5-carboxylate reductaseHAMAPMF_01925P5C_reductasecoord: 11..288
score: 26.129395
IPR029036Pyrroline-5-carboxylate reductase, dimerisation domainPFAMPF14748P5CR_dimercoord: 184..288
e-value: 3.6E-42
score: 142.9
IPR0089276-phosphogluconate dehydrogenase-like, C-terminal domain superfamilySUPERFAMILY481796-phosphogluconate dehydrogenase C-terminal domain-likecoord: 183..290
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 12..181

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh03G001720.1CmoCh03G001720.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055129 L-proline biosynthetic process
biological_process GO:0009408 response to heat
biological_process GO:0009651 response to salt stress
biological_process GO:0006561 proline biosynthetic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004735 pyrroline-5-carboxylate reductase activity