CmoCh02G014500 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh02G014500
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Mannan endo-1,4-beta-mannosidase
Location	Cmo_Chr02: 8534415 .. 8536153 (+)
RNA-Seq Expression	CmoCh02G014500
Synteny	CmoCh02G014500

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAAACTCACTCTCTCTAATTTCTTTAATTCTTTTTTCTTTGTTCGCCTTTTCTCAATCCTTGCCTCTCTCCACTAGTGGAAGATGGATTATAGATGCTCGAACAGGTGATCGTGTGAAGTTGAGTTGTGTCAATTTGGTATCACATGCACAGTCCATGGTTGCTCAAGGACTCGACAAGCGTCCATTGAAAGAGTTGGCGTCTGAGATCACTAGTCGCCAATTCAATTGTGTTCGATTGACTTGGTCTGTTCATATGTTTACTCGTTATGCATATGAGTGTGTTGGTGATGTTTTAGATGGTCTAGATATTGCTGATGTCAAGAGTGGAGTTGCAAAGCACAACCCTGAGATATTGAAAATGACTATTGCTAAGGCGTTTCAAATGGTTATTAATTGTTTGGGCTCGGAAGGCATCATGGTTATTTTAGATAATCATATTAGTCAGCCGAGGTGGTGCTGCTCTCTCGATGATGGCAATGGTTTCTTTGGAGATCGCAATTTCAACCCTAATGAATGGCTTCAAGGCCTGTCATTTGTTGCTGCGCAATTCACTTGTAATCCACATGTGAGTTTTGTGCATTTTAACGGGAACATTTCTATAATATGTATATCGTATTCTAATTTTCTCTTTTTCTTTGTAAAAGGTGGTAGGAATGAGTCTAAGAAACGAGCTTCGAGGGCCATTTACAAACAATGATGCTTGGTGCTATTACATGAGAAGAGGAAGCCAACTAATCCATAGAATAAACCCGAATCTTCTCATAATCATTTCTGGATTAAACTATGACAATGATTTAAGTCAACTAAAGAAAAGGCCACTAGGCTATACCTTTCAGAACAAAGTAGTGTTGGAAGCACATCTCTACTCGTTTAGTGGGGATAACGAAAGCAAATTTGTGAAAAAGCCATTAAACATAATATGCAACGAAATCATGGAGAAGTTTGAACGGGAAGCTGGGTTCGTAGTGGACATGAAGAATCCTTATCCTTTGTTCATTAGTGAATTTGGGTATGATCAATCAGGGGGCAACGAGGCTGAGAATCGGTTCATGAGCTGCTTTTTGGCTCGAATTGCCGAGAAGGATATTGATTGGGCCTTATGGGCATTCCAAGGAAGTTATATGTATAGACAGGGTCATGAAGATCCTGATGAAAGTTTCGGTGTTATGGATCACTCTTGGACTAAAGATCGAAACCCAAAACTTAAACAGATGCTTCAACTTATCAAGAGAGTGAATCAAGGTTAATTTTAACATATCATGCATTTATATATTTCTTTAGTTAGAATTTGAAACTAAAGCTCTTATTTTTGTAGATCTAAATTCTAAAGCACCAATGTCATACATAATATTACATCCCGTGTCTGGGCAATGTGTCAAATCTGACGGCAAAGGAGGAATTGAACTTGGCGATTGTGCCACTCCAACCCAATGGAACCATAGTGGAGATGCAAGTCCGATGAAGTTGTTGTCAAACGGGCAGTGTTTGAAGTCCGCAGGAGATGGCAAATCGCCAATCGTATCCACCGACTGCTCCGGTGACGAATACAATTGGACGGTGGCTTCCAAAGCAAAGCTCCAATTGTCCACAAAAGTTGGCGAAGACAGTTTTTGCTTGGAGAAGGAATCTGATACTTCAATTGTTGTGAAGAAATGTATTTGTCTAGACGCGGAGAGTTGCATGGATGATCCTCAGAGTCAATGGTTCAAGCTCGTTCCAACCAACGTTGCTTAG

mRNA sequence

ATGGCAAACTCACTCTCTCTAATTTCTTTAATTCTTTTTTCTTTGTTCGCCTTTTCTCAATCCTTGCCTCTCTCCACTAGTGGAAGATGGATTATAGATGCTCGAACAGGTGATCGTGTGAAGTTGAGTTGTGTCAATTTGGTATCACATGCACAGTCCATGGTTGCTCAAGGACTCGACAAGCGTCCATTGAAAGAGTTGGCGTCTGAGATCACTAGTCGCCAATTCAATTGTGTTCGATTGACTTGGTCTGTTCATATGTTTACTCGTTATGCATATGAGTGTGTTGGTGATGTTTTAGATGGTCTAGATATTGCTGATGTCAAGAGTGGAGTTGCAAAGCACAACCCTGAGATATTGAAAATGACTATTGCTAAGGCGTTTCAAATGGTTATTAATTGTTTGGGCTCGGAAGGCATCATGGTTATTTTAGATAATCATATTAGTCAGCCGAGGTGGTGCTGCTCTCTCGATGATGGCAATGGTTTCTTTGGAGATCGCAATTTCAACCCTAATGAATGGCTTCAAGGCCTGTCATTTGTTGCTGCGCAATTCACTTGTAATCCACATGTGGTAGGAATGAGTCTAAGAAACGAGCTTCGAGGGCCATTTACAAACAATGATGCTTGGTGCTATTACATGAGAAGAGGAAGCCAACTAATCCATAGAATAAACCCGAATCTTCTCATAATCATTTCTGGATTAAACTATGACAATGATTTAAGTCAACTAAAGAAAAGGCCACTAGGCTATACCTTTCAGAACAAAGTAGTGTTGGAAGCACATCTCTACTCGTTTAGTGGGGATAACGAAAGCAAATTTGTGAAAAAGCCATTAAACATAATATGCAACGAAATCATGGAGAAGTTTGAACGGGAAGCTGGGTTCGTAGTGGACATGAAGAATCCTTATCCTTTGTTCATTAGTGAATTTGGGTATGATCAATCAGGGGGCAACGAGGCTGAGAATCGGTTCATGAGCTGCTTTTTGGCTCGAATTGCCGAGAAGGATATTGATTGGGCCTTATGGGCATTCCAAGGAAGTTATATGTATAGACAGGGTCATGAAGATCCTGATGAAAGTTTCGGTGTTATGGATCACTCTTGGACTAAAGATCGAAACCCAAAACTTAAACAGATGCTTCAACTTATCAAGAGAGTGAATCAAGATCTAAATTCTAAAGCACCAATGTCATACATAATATTACATCCCGTGTCTGGGCAATGTGTCAAATCTGACGGCAAAGGAGGAATTGAACTTGGCGATTGTGCCACTCCAACCCAATGGAACCATAGTGGAGATGCAAGTCCGATGAAGTTGTTGTCAAACGGGCAGTGTTTGAAGTCCGCAGGAGATGGCAAATCGCCAATCGTATCCACCGACTGCTCCGGTGACGAATACAATTGGACGGTGGCTTCCAAAGCAAAGCTCCAATTGTCCACAAAAGTTGGCGAAGACAGTTTTTGCTTGGAGAAGGAATCTGATACTTCAATTGTTGTGAAGAAATGTATTTGTCTAGACGCGGAGAGTTGCATGGATGATCCTCAGAGTCAATGGTTCAAGCTCGTTCCAACCAACGTTGCTTAG

Coding sequence (CDS)

ATGGCAAACTCACTCTCTCTAATTTCTTTAATTCTTTTTTCTTTGTTCGCCTTTTCTCAATCCTTGCCTCTCTCCACTAGTGGAAGATGGATTATAGATGCTCGAACAGGTGATCGTGTGAAGTTGAGTTGTGTCAATTTGGTATCACATGCACAGTCCATGGTTGCTCAAGGACTCGACAAGCGTCCATTGAAAGAGTTGGCGTCTGAGATCACTAGTCGCCAATTCAATTGTGTTCGATTGACTTGGTCTGTTCATATGTTTACTCGTTATGCATATGAGTGTGTTGGTGATGTTTTAGATGGTCTAGATATTGCTGATGTCAAGAGTGGAGTTGCAAAGCACAACCCTGAGATATTGAAAATGACTATTGCTAAGGCGTTTCAAATGGTTATTAATTGTTTGGGCTCGGAAGGCATCATGGTTATTTTAGATAATCATATTAGTCAGCCGAGGTGGTGCTGCTCTCTCGATGATGGCAATGGTTTCTTTGGAGATCGCAATTTCAACCCTAATGAATGGCTTCAAGGCCTGTCATTTGTTGCTGCGCAATTCACTTGTAATCCACATGTGGTAGGAATGAGTCTAAGAAACGAGCTTCGAGGGCCATTTACAAACAATGATGCTTGGTGCTATTACATGAGAAGAGGAAGCCAACTAATCCATAGAATAAACCCGAATCTTCTCATAATCATTTCTGGATTAAACTATGACAATGATTTAAGTCAACTAAAGAAAAGGCCACTAGGCTATACCTTTCAGAACAAAGTAGTGTTGGAAGCACATCTCTACTCGTTTAGTGGGGATAACGAAAGCAAATTTGTGAAAAAGCCATTAAACATAATATGCAACGAAATCATGGAGAAGTTTGAACGGGAAGCTGGGTTCGTAGTGGACATGAAGAATCCTTATCCTTTGTTCATTAGTGAATTTGGGTATGATCAATCAGGGGGCAACGAGGCTGAGAATCGGTTCATGAGCTGCTTTTTGGCTCGAATTGCCGAGAAGGATATTGATTGGGCCTTATGGGCATTCCAAGGAAGTTATATGTATAGACAGGGTCATGAAGATCCTGATGAAAGTTTCGGTGTTATGGATCACTCTTGGACTAAAGATCGAAACCCAAAACTTAAACAGATGCTTCAACTTATCAAGAGAGTGAATCAAGATCTAAATTCTAAAGCACCAATGTCATACATAATATTACATCCCGTGTCTGGGCAATGTGTCAAATCTGACGGCAAAGGAGGAATTGAACTTGGCGATTGTGCCACTCCAACCCAATGGAACCATAGTGGAGATGCAAGTCCGATGAAGTTGTTGTCAAACGGGCAGTGTTTGAAGTCCGCAGGAGATGGCAAATCGCCAATCGTATCCACCGACTGCTCCGGTGACGAATACAATTGGACGGTGGCTTCCAAAGCAAAGCTCCAATTGTCCACAAAAGTTGGCGAAGACAGTTTTTGCTTGGAGAAGGAATCTGATACTTCAATTGTTGTGAAGAAATGTATTTGTCTAGACGCGGAGAGTTGCATGGATGATCCTCAGAGTCAATGGTTCAAGCTCGTTCCAACCAACGTTGCTTAG

Protein sequence

MANSLSLISLILFSLFAFSQSLPLSTSGRWIIDARTGDRVKLSCVNLVSHAQSMVAQGLDKRPLKELASEITSRQFNCVRLTWSVHMFTRYAYECVGDVLDGLDIADVKSGVAKHNPEILKMTIAKAFQMVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNEWLQGLSFVAAQFTCNPHVVGMSLRNELRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIISGLNYDNDLSQLKKRPLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFEREAGFVVDMKNPYPLFISEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQGHEDPDESFGVMDHSWTKDRNPKLKQMLQLIKRVNQDLNSKAPMSYIILHPVSGQCVKSDGKGGIELGDCATPTQWNHSGDASPMKLLSNGQCLKSAGDGKSPIVSTDCSGDEYNWTVASKAKLQLSTKVGEDSFCLEKESDTSIVVKKCICLDAESCMDDPQSQWFKLVPTNVA

Homology

[Close]

BLAST of CmoCh02G014500 vs. ExPASy Swiss-Prot
Match: C0HLA0 (Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 3.4e-116
Identity = 220/538 (40.89%), Postives = 319/538 (59.29%), Query Frame = 0


Query: 7   LISLILFSLFAFSQSLPLSTSGRWIIDARTGDRVKLSCVNLVSHAQSMVAQGLDKRPLKE 66
           L +L+L  + A S SLPL T GRWI+D  TG RVKL+CVN V H +  + +GL++ P+  
Sbjct: 14  LTALLLLLVAAPSHSLPLLTRGRWIVDEATGLRVKLACVNWVGHLEPGLPEGLNRLPVAT 73

Query: 67  LASEITSRQFNCVRLTWSVHMFTRYAY--ECVGDVLDGLDIADVKSGVAKHNPEILKMTI 126
           +A  I+S  FNCVRLT+S+HM TR +Y    V      L++ +  SG+  +NPE+L +  
Sbjct: 74  VAHTISSLGFNCVRLTYSIHMLTRTSYTNATVAQTFARLNLTEAASGIEHNNPELLDLGH 133

Query: 127 AKAFQMVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNEWLQGLSFVAAQ 186
             A+  V+  L   G+MVILDNH+S+P+WCC++DDGNGFFGDR FNPN W++GL  +A  
Sbjct: 134 VAAYHHVVAALSEAGVMVILDNHVSKPKWCCAVDDGNGFFGDRYFNPNTWVEGLGLMATY 193

Query: 187 FTCNPHVVGMSLRNELRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIISGLNYDNDLSQL 246
           F   P+VV MSLRNELRG  +   +W  +M+ G+  +H+ NP +L+I+SGL +D DLS L
Sbjct: 194 FNNTPNVVAMSLRNELRGNRSTPISWSRHMQWGAATVHKANPKVLVILSGLQFDTDLSFL 253

Query: 247 KKRPLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFEREAGFVVDMKN-- 306
              P+   F+ K+V E H YSF     +       N +C     +F+   GFV    N  
Sbjct: 254 PVLPVTLPFKEKIVYEGHWYSFGVPWRTGLP----NDVCKNETGRFKSNVGFVTSSANAT 313

Query: 307 PYPLFISEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYR---QGHEDPD 366
             PLF+SEFG DQ   N+ +NR+++C LA +AE+D+DWALW   GSY YR   Q  +D +
Sbjct: 314 AAPLFMSEFGIDQRYVNDNDNRYLNCILAYLAEEDLDWALWTMGGSYYYRSDKQPVKDFE 373

Query: 367 ESFGVMDHSWTKDRNPKLKQMLQLIKRVNQD-LNSKAPMSYIILHPVSGQCVKSDGKGGI 426
           E++G  +H W++ RNP     L+ I++  QD   +  P   II HP SG CV+S     +
Sbjct: 374 ETYGFFNHDWSRIRNPDFISRLKEIQQPIQDPYLAPGPYYQIIYHPASGLCVESGIGNTV 433

Query: 427 ELGDC-ATPTQWNHSGDA-SPMKLLSNGQCLKSAGDGKSPIVSTDCSGDEYN-WTVASKA 486
            LG C +  ++WN+      P+ L+ +  C+ + G+G   I++ +CS      W+  S A
Sbjct: 434 HLGSCQSVRSRWNYDASVKGPIGLMGSSSCISTQGNGLPAIMTENCSAPNNTLWSTVSSA 493

Query: 487 KLQLSTKV-GEDS----FCLEKESDTSIVVKKCICLDAESCMD--DPQSQWFKLVPTN 527
           +LQL T+V G+D      CL+      I   +CIC+    C    +P+ QWFK++ TN
Sbjct: 494 QLQLGTRVLGKDGKEKWMCLDGSKSPLISTNECICITDSHCYPKLNPEKQWFKVITTN 547

[Close]

BLAST of CmoCh02G014500 vs. ExPASy Swiss-Prot
Match: P19487 (Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) OX=190485 GN=engXCA PE=1 SV=2)

HSP 1 Score: 70.5 bits (171), Expect = 6.5e-11
Identity = 93/397 (23.43%), Postives = 160/397 (40.30%), Query Frame = 0


Query: 2   ANSLSL-ISLILFSLFAFSQSLPLSTSGRWIIDARTGDRVKLSCVNLVS-HAQSMVAQGL 61
           A++L+L  +L L +  AFS S+    + R I+D  +G  V+L  VN+      + V  GL
Sbjct: 7   ASTLALATALALAAGPAFSYSI---NNSRQIVD-DSGKVVQLKGVNVFGFETGNHVMHGL 66

Query: 62  DKRPLKELASEITSRQFNCVRLTWSVHMFTRYAYECVGDVLDGLDIADVKSGVAKHNPEI 121
             R  K++  ++    FN VRL +           C   +      A +       N ++
Sbjct: 67  WARNWKDMIVQMQGLGFNAVRLPF-----------CPATLRSDTMPASID---YSRNADL 126

Query: 122 LKMTIAKAFQMVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNEWLQGLS 181
             +T  +    VI    + G+ V+LD+H      C  + +    +   ++   +WL  L 
Sbjct: 127 QGLTSLQILDKVIAEFNARGMYVLLDHHTPD---CAGISE---LWYTGSYTEAQWLADLR 186

Query: 182 FVAAQFTCNPHVVGMSLRNELRGPF---TNNDA--WCYYMRRGSQLIHRINPNLLIIISG 241
           FVA ++   P+V+G+ L+NE  G     T N A  W     RGS  +  + P  LI + G
Sbjct: 187 FVANRYKNVPYVLGLDLKNEPHGAATWGTGNAATDWNKAAERGSAAVLAVAPKWLIAVEG 246

Query: 242 LN------------YDNDLSQLKKRPLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLN-- 301
           +             +  +L  L   PL     N+++L  H+Y         FV+   N  
Sbjct: 247 ITDNPVCSTNGGIFWGGNLQPLACTPLNIP-ANRLLLAPHVY-----GPDVFVQSYFNDS 306

Query: 302 IICNEIMEKFEREAGFVVDMKNPYPLFISEFGYDQSGGNEAENRFMSCFLARIAEKDIDW 361
              N +   +ER  G        + L + EFG     G+  +  +    +  +  K I+ 
Sbjct: 307 NFPNNMPAIWERHFG---QFAGTHALLLGEFGGKYGEGDARDKTWQDALVKYLRSKGIN- 362

Query: 362 ALWAFQGSYMYRQGHEDPDESFGVMDHSWTKDRNPKL 378
                QG   Y   + +  ++ G++   WT  R  K+
Sbjct: 367 -----QG--FYWSWNPNSGDTGGILRDDWTSVRQDKM 362

[Close]

BLAST of CmoCh02G014500 vs. ExPASy TrEMBL
Match: A0A6J1GZR4 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111459103 PE=3 SV=1)

HSP 1 Score: 1000.0 bits (2584), Expect = 3.8e-288
Identity = 475/475 (100.00%), Postives = 475/475 (100.00%), Query Frame = 0


Query: 54  MVAQGLDKRPLKELASEITSRQFNCVRLTWSVHMFTRYAYECVGDVLDGLDIADVKSGVA 113
           MVAQGLDKRPLKELASEITSRQFNCVRLTWSVHMFTRYAYECVGDVLDGLDIADVKSGVA
Sbjct: 1   MVAQGLDKRPLKELASEITSRQFNCVRLTWSVHMFTRYAYECVGDVLDGLDIADVKSGVA 60

Query: 114 KHNPEILKMTIAKAFQMVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE 173
           KHNPEILKMTIAKAFQMVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE
Sbjct: 61  KHNPEILKMTIAKAFQMVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE 120

Query: 174 WLQGLSFVAAQFTCNPHVVGMSLRNELRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIIS 233
           WLQGLSFVAAQFTCNPHVVGMSLRNELRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIIS
Sbjct: 121 WLQGLSFVAAQFTCNPHVVGMSLRNELRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIIS 180

Query: 234 GLNYDNDLSQLKKRPLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFERE 293
           GLNYDNDLSQLKKRPLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFERE
Sbjct: 181 GLNYDNDLSQLKKRPLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFERE 240

Query: 294 AGFVVDMKNPYPLFISEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQ 353
           AGFVVDMKNPYPLFISEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQ
Sbjct: 241 AGFVVDMKNPYPLFISEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQ 300

Query: 354 GHEDPDESFGVMDHSWTKDRNPKLKQMLQLIKRVNQDLNSKAPMSYIILHPVSGQCVKSD 413
           GHEDPDESFGVMDHSWTKDRNPKLKQMLQLIKRVNQDLNSKAPMSYIILHPVSGQCVKSD
Sbjct: 301 GHEDPDESFGVMDHSWTKDRNPKLKQMLQLIKRVNQDLNSKAPMSYIILHPVSGQCVKSD 360

Query: 414 GKGGIELGDCATPTQWNHSGDASPMKLLSNGQCLKSAGDGKSPIVSTDCSGDEYNWTVAS 473
           GKGGIELGDCATPTQWNHSGDASPMKLLSNGQCLKSAGDGKSPIVSTDCSGDEYNWTVAS
Sbjct: 361 GKGGIELGDCATPTQWNHSGDASPMKLLSNGQCLKSAGDGKSPIVSTDCSGDEYNWTVAS 420

Query: 474 KAKLQLSTKVGEDSFCLEKESDTSIVVKKCICLDAESCMDDPQSQWFKLVPTNVA 529
           KAKLQLSTKVGEDSFCLEKESDTSIVVKKCICLDAESCMDDPQSQWFKLVPTNVA
Sbjct: 421 KAKLQLSTKVGEDSFCLEKESDTSIVVKKCICLDAESCMDDPQSQWFKLVPTNVA 475

[Close]

BLAST of CmoCh02G014500 vs. ExPASy TrEMBL
Match: A0A6J1K7T8 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111491432 PE=3 SV=1)

HSP 1 Score: 985.7 bits (2547), Expect = 7.4e-284
Identity = 465/475 (97.89%), Postives = 472/475 (99.37%), Query Frame = 0


Query: 54  MVAQGLDKRPLKELASEITSRQFNCVRLTWSVHMFTRYAYECVGDVLDGLDIADVKSGVA 113
           MVAQGLDKRPLKELASEITSRQFNCVRLTWSV+MFTRYAYECVGDVLDGLDIADVK+GVA
Sbjct: 1   MVAQGLDKRPLKELASEITSRQFNCVRLTWSVYMFTRYAYECVGDVLDGLDIADVKTGVA 60

Query: 114 KHNPEILKMTIAKAFQMVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE 173
           KHNPEILKMT+AKAFQ VINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE
Sbjct: 61  KHNPEILKMTVAKAFQTVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE 120

Query: 174 WLQGLSFVAAQFTCNPHVVGMSLRNELRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIIS 233
           WLQGLSFVAAQFTCNPHV+GMSLRNELRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIIS
Sbjct: 121 WLQGLSFVAAQFTCNPHVIGMSLRNELRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIIS 180

Query: 234 GLNYDNDLSQLKKRPLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFERE 293
           GLNY NDLSQLKK+PLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFERE
Sbjct: 181 GLNYGNDLSQLKKKPLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFERE 240

Query: 294 AGFVVDMKNPYPLFISEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQ 353
           AGFVVDMKNPYPLFISEFGYDQSG NEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQ
Sbjct: 241 AGFVVDMKNPYPLFISEFGYDQSGSNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQ 300

Query: 354 GHEDPDESFGVMDHSWTKDRNPKLKQMLQLIKRVNQDLNSKAPMSYIILHPVSGQCVKSD 413
           GHEDPDESFGVMDHSWTKDRNPKLKQMLQLIKRVNQDLNSKAPMSYI+LHPVSGQCVKSD
Sbjct: 301 GHEDPDESFGVMDHSWTKDRNPKLKQMLQLIKRVNQDLNSKAPMSYIMLHPVSGQCVKSD 360

Query: 414 GKGGIELGDCATPTQWNHSGDASPMKLLSNGQCLKSAGDGKSPIVSTDCSGDEYNWTVAS 473
           GKGGIELGDCATPTQWNHSGDASPMKLLSNGQCLKSAGDGKSPIVSTDCSGDEYNWTVAS
Sbjct: 361 GKGGIELGDCATPTQWNHSGDASPMKLLSNGQCLKSAGDGKSPIVSTDCSGDEYNWTVAS 420

Query: 474 KAKLQLSTKVGEDSFCLEKESDTSIVVKKCICLDAESCMDDPQSQWFKLVPTNVA 529
           KAKLQLSTKVGEDSFCLEKESDTSI+VKKCICLDAESCMDDPQSQWFKLVPTNVA
Sbjct: 421 KAKLQLSTKVGEDSFCLEKESDTSIIVKKCICLDAESCMDDPQSQWFKLVPTNVA 475

[Close]

BLAST of CmoCh02G014500 vs. ExPASy TrEMBL
Match: A0A6J1H0Z1 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111459153 PE=3 SV=1)

HSP 1 Score: 980.3 bits (2533), Expect = 3.1e-282
Identity = 464/475 (97.68%), Postives = 470/475 (98.95%), Query Frame = 0


Query: 54  MVAQGLDKRPLKELASEITSRQFNCVRLTWSVHMFTRYAYECVGDVLDGLDIADVKSGVA 113
           MVAQGLDKRPLKELASEIT+RQFNCVRLTWSVHMFTRYAYECVGDVLDGLDIADVKSGVA
Sbjct: 1   MVAQGLDKRPLKELASEITNRQFNCVRLTWSVHMFTRYAYECVGDVLDGLDIADVKSGVA 60

Query: 114 KHNPEILKMTIAKAFQMVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE 173
           KHNPEILKMT+AKAFQ VINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE
Sbjct: 61  KHNPEILKMTVAKAFQTVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE 120

Query: 174 WLQGLSFVAAQFTCNPHVVGMSLRNELRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIIS 233
           WLQGLSFVAAQFTCNPHVVGMSLRNELRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIIS
Sbjct: 121 WLQGLSFVAAQFTCNPHVVGMSLRNELRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIIS 180

Query: 234 GLNYDNDLSQLKKRPLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFERE 293
           GLNY NDLSQLKKRPLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFERE
Sbjct: 181 GLNYGNDLSQLKKRPLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFERE 240

Query: 294 AGFVVDMKNPYPLFISEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQ 353
           AGFVVDMKNPYPLFISEFGY+QSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQ
Sbjct: 241 AGFVVDMKNPYPLFISEFGYNQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQ 300

Query: 354 GHEDPDESFGVMDHSWTKDRNPKLKQMLQLIKRVNQDLNSKAPMSYIILHPVSGQCVKSD 413
           GH DPDESFGVMDHSWTKDRNPKLKQMLQLIKRVNQDLNS APMSYI+LHPVSGQCVKSD
Sbjct: 301 GHVDPDESFGVMDHSWTKDRNPKLKQMLQLIKRVNQDLNSNAPMSYIMLHPVSGQCVKSD 360

Query: 414 GKGGIELGDCATPTQWNHSGDASPMKLLSNGQCLKSAGDGKSPIVSTDCSGDEYNWTVAS 473
           GKGGIELGDCATPTQWNHSGDASPMKLLSNGQCLKSAGDGKSPIVS DCSGDEYNWTVAS
Sbjct: 361 GKGGIELGDCATPTQWNHSGDASPMKLLSNGQCLKSAGDGKSPIVSIDCSGDEYNWTVAS 420

Query: 474 KAKLQLSTKVGEDSFCLEKESDTSIVVKKCICLDAESCMDDPQSQWFKLVPTNVA 529
           KAKLQLSTKVGE+SFCLEKES+TSIVVKKCICLDAESCMDDPQSQWFKLVPTNVA
Sbjct: 421 KAKLQLSTKVGEESFCLEKESETSIVVKKCICLDAESCMDDPQSQWFKLVPTNVA 475

[Close]

BLAST of CmoCh02G014500 vs. ExPASy TrEMBL
Match: A0A6J1H187 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111459155 PE=3 SV=1)

HSP 1 Score: 976.5 bits (2523), Expect = 4.5e-281
Identity = 461/475 (97.05%), Postives = 469/475 (98.74%), Query Frame = 0


Query: 54  MVAQGLDKRPLKELASEITSRQFNCVRLTWSVHMFTRYAYECVGDVLDGLDIADVKSGVA 113
           MVAQGLDKRPLKEL SEI+SR+FNCVRLTWSVHMFTRYAYECVGDVLDGLDIADVKSGVA
Sbjct: 1   MVAQGLDKRPLKELVSEISSRKFNCVRLTWSVHMFTRYAYECVGDVLDGLDIADVKSGVA 60

Query: 114 KHNPEILKMTIAKAFQMVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE 173
           KHNPEILKMT+AKAFQ VINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE
Sbjct: 61  KHNPEILKMTVAKAFQTVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE 120

Query: 174 WLQGLSFVAAQFTCNPHVVGMSLRNELRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIIS 233
           WLQGLSFVAAQFTCNPHVVGMSLRNELRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIIS
Sbjct: 121 WLQGLSFVAAQFTCNPHVVGMSLRNELRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIIS 180

Query: 234 GLNYDNDLSQLKKRPLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFERE 293
           GLNY NDLSQLKKRPLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFERE
Sbjct: 181 GLNYGNDLSQLKKRPLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFERE 240

Query: 294 AGFVVDMKNPYPLFISEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQ 353
           AGFVVDMKNPYPLFISEFGYDQSG NEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQ
Sbjct: 241 AGFVVDMKNPYPLFISEFGYDQSGSNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQ 300

Query: 354 GHEDPDESFGVMDHSWTKDRNPKLKQMLQLIKRVNQDLNSKAPMSYIILHPVSGQCVKSD 413
           GH DPDESFGVMDHSWTKDRNPKLKQMLQLIKRVNQDLNS APMSYI+LHPVSGQCVKSD
Sbjct: 301 GHVDPDESFGVMDHSWTKDRNPKLKQMLQLIKRVNQDLNSNAPMSYIMLHPVSGQCVKSD 360

Query: 414 GKGGIELGDCATPTQWNHSGDASPMKLLSNGQCLKSAGDGKSPIVSTDCSGDEYNWTVAS 473
           GKGGIELGDCATPTQWNHSGDASPMKLLSNGQCLKSAGDGKSPIVST+CSGDEYNWTVAS
Sbjct: 361 GKGGIELGDCATPTQWNHSGDASPMKLLSNGQCLKSAGDGKSPIVSTNCSGDEYNWTVAS 420

Query: 474 KAKLQLSTKVGEDSFCLEKESDTSIVVKKCICLDAESCMDDPQSQWFKLVPTNVA 529
           KAKLQLSTKVGE+SFCLEKES+TSIVVKKC+CLDAESCMDDPQSQWFKLVPTNVA
Sbjct: 421 KAKLQLSTKVGEESFCLEKESETSIVVKKCVCLDAESCMDDPQSQWFKLVPTNVA 475

[Close]

BLAST of CmoCh02G014500 vs. ExPASy TrEMBL
Match: A0A6J1JQD4 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111487386 PE=3 SV=1)

HSP 1 Score: 927.9 bits (2397), Expect = 1.8e-266
Identity = 427/475 (89.89%), Postives = 454/475 (95.58%), Query Frame = 0


Query: 54  MVAQGLDKRPLKELASEITSRQFNCVRLTWSVHMFTRYAYECVGDVLDGLDIADVKSGVA 113
           MVAQGLDKRPLKELA+EI+SRQFNCVRLTWS+HMFTRYAYE +GDVLDGLDI DVK+GV 
Sbjct: 1   MVAQGLDKRPLKELAAEISSRQFNCVRLTWSIHMFTRYAYETIGDVLDGLDIGDVKNGVE 60

Query: 114 KHNPEILKMTIAKAFQMVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE 173
           KHNPEILKM++ KAFQ VINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE
Sbjct: 61  KHNPEILKMSVVKAFQTVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE 120

Query: 174 WLQGLSFVAAQFTCNPHVVGMSLRNELRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIIS 233
           WLQGLSFVA QFTCNPHV+GMSLRNELRGPFTNNDAWCYYMR+GSQLIHRINPN+L+IIS
Sbjct: 121 WLQGLSFVATQFTCNPHVIGMSLRNELRGPFTNNDAWCYYMRQGSQLIHRINPNILVIIS 180

Query: 234 GLNYDNDLSQLKKRPLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFERE 293
           GLNYDNDLS LKKRPLGYT  NKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFERE
Sbjct: 181 GLNYDNDLSYLKKRPLGYTLHNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFERE 240

Query: 294 AGFVVDMKNPYPLFISEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQ 353
           AGFVVDM+NPYPLF+SEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQ
Sbjct: 241 AGFVVDMENPYPLFLSEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQ 300

Query: 354 GHEDPDESFGVMDHSWTKDRNPKLKQMLQLIKRVNQDLNSKAPMSYIILHPVSGQCVKSD 413
           GH DPDESFGVMDHSWTKDRNPKL Q+LQL+KR+NQD NSKAPMSYI+LH VSGQCVKSD
Sbjct: 301 GHTDPDESFGVMDHSWTKDRNPKLAQLLQLVKRINQDPNSKAPMSYIVLHSVSGQCVKSD 360

Query: 414 GKGGIELGDCATPTQWNHSGDASPMKLLSNGQCLKSAGDGKSPIVSTDCSGDEYNWTVAS 473
            KGGIELGDC  PTQWNH+GDASPMKLLSNGQCLKSAGDGKSP+VS +CSGD  NWTV+S
Sbjct: 361 NKGGIELGDCTAPTQWNHTGDASPMKLLSNGQCLKSAGDGKSPVVSAECSGDGSNWTVSS 420

Query: 474 KAKLQLSTKVGEDSFCLEKESDTSIVVKKCICLDAESCMDDPQSQWFKLVPTNVA 529
           KAKLQL+TK+GED+FCLEKESD SIV+KKCICLD ESCMDDPQ+QWFKLVPTNV+
Sbjct: 421 KAKLQLATKIGEDNFCLEKESDASIVLKKCICLDEESCMDDPQTQWFKLVPTNVS 475

[Close]

BLAST of CmoCh02G014500 vs. NCBI nr
Match: KAG6601845.1 (hypothetical protein SDJN03_07078, partial [Cucurbita argyrosperma subsp. sororia] >KAG6601853.1 hypothetical protein SDJN03_07086, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1001.9 bits (2589), Expect = 2.1e-288
Identity = 469/528 (88.83%), Postives = 502/528 (95.08%), Query Frame = 0


Query: 1   MANSLSLISLILFSLFAFSQSLPLSTSGRWIIDARTGDRVKLSCVNLVSHAQSMVAQGLD 60
           MA S SLI+LILFSLF+FSQSLPL T GRWIIDA+TG RVKL+CVNLVSHAQ+MVAQGLD
Sbjct: 1   MAKSFSLIALILFSLFSFSQSLPLCTRGRWIIDAQTGKRVKLNCVNLVSHAQTMVAQGLD 60

Query: 61  KRPLKELASEITSRQFNCVRLTWSVHMFTRYAYECVGDVLDGLDIADVKSGVAKHNPEIL 120
           KRPLKELA+EI+SRQFNCVRLTWSVHMFTRYAYE +GDVLDGLDI DVK+GV KHNPEIL
Sbjct: 61  KRPLKELAAEISSRQFNCVRLTWSVHMFTRYAYETIGDVLDGLDIGDVKNGVEKHNPEIL 120

Query: 121 KMTIAKAFQMVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNEWLQGLSF 180
           KM++AKAFQ VINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNEW QGLSF
Sbjct: 121 KMSVAKAFQTVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNEWFQGLSF 180

Query: 181 VAAQFTCNPHVVGMSLRNELRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIISGLNYDND 240
           VA QFTCNPHV+GMSLRNELRGPFTNNDAWCYYMR+GSQLIHRINPN+LIIISGLNYDND
Sbjct: 181 VATQFTCNPHVIGMSLRNELRGPFTNNDAWCYYMRQGSQLIHRINPNILIIISGLNYDND 240

Query: 241 LSQLKKRPLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFEREAGFVVDM 300
           LS LKKRPLGYT  NKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFEREAGFVVDM
Sbjct: 241 LSYLKKRPLGYTLHNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFEREAGFVVDM 300

Query: 301 KNPYPLFISEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQGHEDPDE 360
           +NPYPLF+SEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQGH DPDE
Sbjct: 301 ENPYPLFLSEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQGHTDPDE 360

Query: 361 SFGVMDHSWTKDRNPKLKQMLQLIKRVNQDLNSKAPMSYIILHPVSGQCVKSDGKGGIEL 420
           SFGVMDHSWTKDRNPKL Q+LQL+KR+NQD NSKAPMSYI+LH VSGQCV+SD KGGIEL
Sbjct: 361 SFGVMDHSWTKDRNPKLAQLLQLVKRINQDPNSKAPMSYIMLHSVSGQCVRSDNKGGIEL 420

Query: 421 GDCATPTQWNHSGDASPMKLLSNGQCLKSAGDGKSPIVSTDCSGDEYNWTVASKAKLQLS 480
           GDC +PTQWNH+GDASPM LLSNGQCLKSAGDGKSP+VS +CSGD  NWTV+SKAKLQL+
Sbjct: 421 GDCTSPTQWNHTGDASPMMLLSNGQCLKSAGDGKSPMVSAECSGDGSNWTVSSKAKLQLA 480

Query: 481 TKVGEDSFCLEKESDTSIVVKKCICLDAESCMDDPQSQWFKLVPTNVA 529
           TK+GED+FCLEKES+ SIV+KKCICLD ESCMDDPQ+QWFKLVPTNV+
Sbjct: 481 TKIGEDNFCLEKESNASIVLKKCICLDEESCMDDPQTQWFKLVPTNVS 528

[Close]

BLAST of CmoCh02G014500 vs. NCBI nr
Match: XP_022957626.1 (uncharacterized protein LOC111459103 [Cucurbita moschata])

HSP 1 Score: 1000.0 bits (2584), Expect = 7.9e-288
Identity = 475/475 (100.00%), Postives = 475/475 (100.00%), Query Frame = 0


Query: 54  MVAQGLDKRPLKELASEITSRQFNCVRLTWSVHMFTRYAYECVGDVLDGLDIADVKSGVA 113
           MVAQGLDKRPLKELASEITSRQFNCVRLTWSVHMFTRYAYECVGDVLDGLDIADVKSGVA
Sbjct: 1   MVAQGLDKRPLKELASEITSRQFNCVRLTWSVHMFTRYAYECVGDVLDGLDIADVKSGVA 60

Query: 114 KHNPEILKMTIAKAFQMVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE 173
           KHNPEILKMTIAKAFQMVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE
Sbjct: 61  KHNPEILKMTIAKAFQMVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE 120

Query: 174 WLQGLSFVAAQFTCNPHVVGMSLRNELRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIIS 233
           WLQGLSFVAAQFTCNPHVVGMSLRNELRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIIS
Sbjct: 121 WLQGLSFVAAQFTCNPHVVGMSLRNELRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIIS 180

Query: 234 GLNYDNDLSQLKKRPLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFERE 293
           GLNYDNDLSQLKKRPLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFERE
Sbjct: 181 GLNYDNDLSQLKKRPLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFERE 240

Query: 294 AGFVVDMKNPYPLFISEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQ 353
           AGFVVDMKNPYPLFISEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQ
Sbjct: 241 AGFVVDMKNPYPLFISEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQ 300

Query: 354 GHEDPDESFGVMDHSWTKDRNPKLKQMLQLIKRVNQDLNSKAPMSYIILHPVSGQCVKSD 413
           GHEDPDESFGVMDHSWTKDRNPKLKQMLQLIKRVNQDLNSKAPMSYIILHPVSGQCVKSD
Sbjct: 301 GHEDPDESFGVMDHSWTKDRNPKLKQMLQLIKRVNQDLNSKAPMSYIILHPVSGQCVKSD 360

Query: 414 GKGGIELGDCATPTQWNHSGDASPMKLLSNGQCLKSAGDGKSPIVSTDCSGDEYNWTVAS 473
           GKGGIELGDCATPTQWNHSGDASPMKLLSNGQCLKSAGDGKSPIVSTDCSGDEYNWTVAS
Sbjct: 361 GKGGIELGDCATPTQWNHSGDASPMKLLSNGQCLKSAGDGKSPIVSTDCSGDEYNWTVAS 420

Query: 474 KAKLQLSTKVGEDSFCLEKESDTSIVVKKCICLDAESCMDDPQSQWFKLVPTNVA 529
           KAKLQLSTKVGEDSFCLEKESDTSIVVKKCICLDAESCMDDPQSQWFKLVPTNVA
Sbjct: 421 KAKLQLSTKVGEDSFCLEKESDTSIVVKKCICLDAESCMDDPQSQWFKLVPTNVA 475

[Close]

BLAST of CmoCh02G014500 vs. NCBI nr
Match: XP_022996114.1 (uncharacterized protein LOC111491432 [Cucurbita maxima])

HSP 1 Score: 985.7 bits (2547), Expect = 1.5e-283
Identity = 465/475 (97.89%), Postives = 472/475 (99.37%), Query Frame = 0


Query: 54  MVAQGLDKRPLKELASEITSRQFNCVRLTWSVHMFTRYAYECVGDVLDGLDIADVKSGVA 113
           MVAQGLDKRPLKELASEITSRQFNCVRLTWSV+MFTRYAYECVGDVLDGLDIADVK+GVA
Sbjct: 1   MVAQGLDKRPLKELASEITSRQFNCVRLTWSVYMFTRYAYECVGDVLDGLDIADVKTGVA 60

Query: 114 KHNPEILKMTIAKAFQMVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE 173
           KHNPEILKMT+AKAFQ VINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE
Sbjct: 61  KHNPEILKMTVAKAFQTVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE 120

Query: 174 WLQGLSFVAAQFTCNPHVVGMSLRNELRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIIS 233
           WLQGLSFVAAQFTCNPHV+GMSLRNELRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIIS
Sbjct: 121 WLQGLSFVAAQFTCNPHVIGMSLRNELRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIIS 180

Query: 234 GLNYDNDLSQLKKRPLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFERE 293
           GLNY NDLSQLKK+PLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFERE
Sbjct: 181 GLNYGNDLSQLKKKPLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFERE 240

Query: 294 AGFVVDMKNPYPLFISEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQ 353
           AGFVVDMKNPYPLFISEFGYDQSG NEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQ
Sbjct: 241 AGFVVDMKNPYPLFISEFGYDQSGSNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQ 300

Query: 354 GHEDPDESFGVMDHSWTKDRNPKLKQMLQLIKRVNQDLNSKAPMSYIILHPVSGQCVKSD 413
           GHEDPDESFGVMDHSWTKDRNPKLKQMLQLIKRVNQDLNSKAPMSYI+LHPVSGQCVKSD
Sbjct: 301 GHEDPDESFGVMDHSWTKDRNPKLKQMLQLIKRVNQDLNSKAPMSYIMLHPVSGQCVKSD 360

Query: 414 GKGGIELGDCATPTQWNHSGDASPMKLLSNGQCLKSAGDGKSPIVSTDCSGDEYNWTVAS 473
           GKGGIELGDCATPTQWNHSGDASPMKLLSNGQCLKSAGDGKSPIVSTDCSGDEYNWTVAS
Sbjct: 361 GKGGIELGDCATPTQWNHSGDASPMKLLSNGQCLKSAGDGKSPIVSTDCSGDEYNWTVAS 420

Query: 474 KAKLQLSTKVGEDSFCLEKESDTSIVVKKCICLDAESCMDDPQSQWFKLVPTNVA 529
           KAKLQLSTKVGEDSFCLEKESDTSI+VKKCICLDAESCMDDPQSQWFKLVPTNVA
Sbjct: 421 KAKLQLSTKVGEDSFCLEKESDTSIIVKKCICLDAESCMDDPQSQWFKLVPTNVA 475

[Close]

BLAST of CmoCh02G014500 vs. NCBI nr
Match: KAG6606063.1 (hypothetical protein SDJN03_03380, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 980.7 bits (2534), Expect = 4.9e-282
Identity = 465/475 (97.89%), Postives = 467/475 (98.32%), Query Frame = 0


Query: 54  MVAQGLDKRPLKELASEITSRQFNCVRLTWSVHMFTRYAYECVGDVLDGLDIADVKSGVA 113
           MVAQGLDKRPLKELASEITSRQFNCVRLTWSVHMFTRYAYECVGDVLDGLDIADVKSGVA
Sbjct: 1   MVAQGLDKRPLKELASEITSRQFNCVRLTWSVHMFTRYAYECVGDVLDGLDIADVKSGVA 60

Query: 114 KHNPEILKMTIAKAFQMVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE 173
           KHNPEILKMT+AKAFQ VINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE
Sbjct: 61  KHNPEILKMTVAKAFQTVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE 120

Query: 174 WLQGLSFVAAQFTCNPHVVGMSLRNELRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIIS 233
           W QGLSFVA QFTCNPHVVGMSLRNELRGPFTNNDAWCYYMRRGSQLIHRIN NLLIIIS
Sbjct: 121 WFQGLSFVAVQFTCNPHVVGMSLRNELRGPFTNNDAWCYYMRRGSQLIHRINQNLLIIIS 180

Query: 234 GLNYDNDLSQLKKRPLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFERE 293
           GLNY NDLSQLKKRPLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFERE
Sbjct: 181 GLNYGNDLSQLKKRPLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFERE 240

Query: 294 AGFVVDMKNPYPLFISEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQ 353
           AGFVVDMKNPYPLFISEFGYDQSG NEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQ
Sbjct: 241 AGFVVDMKNPYPLFISEFGYDQSGSNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQ 300

Query: 354 GHEDPDESFGVMDHSWTKDRNPKLKQMLQLIKRVNQDLNSKAPMSYIILHPVSGQCVKSD 413
           GHEDPDESFGVMDH+WTKDRNPKLKQMLQLIKRVNQDLNS APMSYIILHPVSGQCVKSD
Sbjct: 301 GHEDPDESFGVMDHTWTKDRNPKLKQMLQLIKRVNQDLNSNAPMSYIILHPVSGQCVKSD 360

Query: 414 GKGGIELGDCATPTQWNHSGDASPMKLLSNGQCLKSAGDGKSPIVSTDCSGDEYNWTVAS 473
           GKGGIELGDCATPTQWNHSGDASPMKLLSNGQCLKSAGDGKSPIVSTDCSGDEYNWTVAS
Sbjct: 361 GKGGIELGDCATPTQWNHSGDASPMKLLSNGQCLKSAGDGKSPIVSTDCSGDEYNWTVAS 420

Query: 474 KAKLQLSTKVGEDSFCLEKESDTSIVVKKCICLDAESCMDDPQSQWFKLVPTNVA 529
           KAKLQLSTKVGEDSFCL KESDTSIVVKKCICLDAESCMDDPQSQWFKLVPTNVA
Sbjct: 421 KAKLQLSTKVGEDSFCLAKESDTSIVVKKCICLDAESCMDDPQSQWFKLVPTNVA 475

[Close]

BLAST of CmoCh02G014500 vs. NCBI nr
Match: XP_022957688.1 (uncharacterized protein LOC111459153 [Cucurbita moschata])

HSP 1 Score: 980.3 bits (2533), Expect = 6.4e-282
Identity = 464/475 (97.68%), Postives = 470/475 (98.95%), Query Frame = 0


Query: 54  MVAQGLDKRPLKELASEITSRQFNCVRLTWSVHMFTRYAYECVGDVLDGLDIADVKSGVA 113
           MVAQGLDKRPLKELASEIT+RQFNCVRLTWSVHMFTRYAYECVGDVLDGLDIADVKSGVA
Sbjct: 1   MVAQGLDKRPLKELASEITNRQFNCVRLTWSVHMFTRYAYECVGDVLDGLDIADVKSGVA 60

Query: 114 KHNPEILKMTIAKAFQMVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE 173
           KHNPEILKMT+AKAFQ VINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE
Sbjct: 61  KHNPEILKMTVAKAFQTVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE 120

Query: 174 WLQGLSFVAAQFTCNPHVVGMSLRNELRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIIS 233
           WLQGLSFVAAQFTCNPHVVGMSLRNELRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIIS
Sbjct: 121 WLQGLSFVAAQFTCNPHVVGMSLRNELRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIIS 180

Query: 234 GLNYDNDLSQLKKRPLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFERE 293
           GLNY NDLSQLKKRPLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFERE
Sbjct: 181 GLNYGNDLSQLKKRPLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFERE 240

Query: 294 AGFVVDMKNPYPLFISEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQ 353
           AGFVVDMKNPYPLFISEFGY+QSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQ
Sbjct: 241 AGFVVDMKNPYPLFISEFGYNQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQ 300

Query: 354 GHEDPDESFGVMDHSWTKDRNPKLKQMLQLIKRVNQDLNSKAPMSYIILHPVSGQCVKSD 413
           GH DPDESFGVMDHSWTKDRNPKLKQMLQLIKRVNQDLNS APMSYI+LHPVSGQCVKSD
Sbjct: 301 GHVDPDESFGVMDHSWTKDRNPKLKQMLQLIKRVNQDLNSNAPMSYIMLHPVSGQCVKSD 360

Query: 414 GKGGIELGDCATPTQWNHSGDASPMKLLSNGQCLKSAGDGKSPIVSTDCSGDEYNWTVAS 473
           GKGGIELGDCATPTQWNHSGDASPMKLLSNGQCLKSAGDGKSPIVS DCSGDEYNWTVAS
Sbjct: 361 GKGGIELGDCATPTQWNHSGDASPMKLLSNGQCLKSAGDGKSPIVSIDCSGDEYNWTVAS 420

Query: 474 KAKLQLSTKVGEDSFCLEKESDTSIVVKKCICLDAESCMDDPQSQWFKLVPTNVA 529
           KAKLQLSTKVGE+SFCLEKES+TSIVVKKCICLDAESCMDDPQSQWFKLVPTNVA
Sbjct: 421 KAKLQLSTKVGEESFCLEKESETSIVVKKCICLDAESCMDDPQSQWFKLVPTNVA 475

[Close]

BLAST of CmoCh02G014500 vs. TAIR 10
Match: AT3G26130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 401.0 bits (1029), Expect = 1.5e-111
Identity = 226/530 (42.64%), Postives = 308/530 (58.11%), Query Frame = 0


Query: 8   ISLILFSLFAFSQSLPLSTSGRWII-DARTGDRVKLSCVNLVSHAQSMVAQGLDKRPLKE 67
           IS+ L      + + P ST  RWI+ D   G RVKL+CVN  SH ++ VA+GL K+PL  
Sbjct: 7   ISVFLLPYVITTFAFPPSTDSRWIVDDGNKGRRVKLTCVNWPSHLETAVAEGLSKQPLDA 66

Query: 68  LASEITSRQFNCVRLTWSVHMFTRYAYECVGDVLDGL---DIADVKSGVAKHNPEILKMT 127
           +A +I S  FNCVRLTW +++ T  ++     V   L    + +  SG   HNP IL + 
Sbjct: 67  IAEKIVSMGFNCVRLTWPLYLATDESFSAFMTVRQSLRKFRLFEAVSGFQTHNPTILDLP 126

Query: 128 IAKAFQMVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNEWLQGLSFVAA 187
           + KAFQ V+ CL    +MVILDNHISQP WCCS +DGNGFFGD++ NP  W++GL  +A+
Sbjct: 127 LIKAFQEVVYCLEKHRVMVILDNHISQPGWCCSDNDGNGFFGDKHLNPQVWIKGLKKMAS 186

Query: 188 QF-TCNPHVVGMSLRNELRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIISGLNYDNDLS 247
            F   + +VVGMSLRNELRGP  N   W  YMR G++ +H +NPN+L+I+SGLNY  DLS
Sbjct: 187 MFANVSSNVVGMSLRNELRGPKQNIKDWYKYMREGAEAVHSVNPNVLVIVSGLNYATDLS 246

Query: 248 QLKKRPLGYTFQNKVVLEAHLYSF----SGDNESKFVKKPLNIICNEIMEKFEREAGFVV 307
            L++RP   +F+ KVV E H Y F     GDN        LN IC +  EK  + +GF+ 
Sbjct: 247 FLRERPFEVSFRRKVVFEIHWYGFWNTWEGDN--------LNKICGKETEKMMKMSGFL- 306

Query: 308 DMKNPYPLFISEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQGHEDP 367
            ++   PLF+SEFG DQ G N  +N+F+SCF+A  A++D+DW+LW   GSY  R+     
Sbjct: 307 -LEKGIPLFVSEFGIDQRGNNANDNKFLSCFMALAADRDLDWSLWTLAGSYYIREKSIGS 366

Query: 368 DESFGVMDHSWTKDRNPKLKQMLQLIKRVNQDLNSKAPMSYIILHPVSGQCVKSDGKGGI 427
           DES+GV+D +W+  RN  + QM+  I+     L    P   I+ HP +G C+       +
Sbjct: 367 DESYGVLDFNWSSIRNSTILQMISAIQTPFIGLMETQPKK-IMFHPSTGLCIVRKSLFQL 426

Query: 428 ELGDCATPTQWNHSGDASPMKLLSNGQCLKSAGDGKSPIVSTDCSGDEY--NWTVASKAK 487
           +LG C     W  S             CLK+   GKS  +    S + Y   W + S +K
Sbjct: 427 KLGSCNRSESWRLSSHRVLSLAEEQILCLKAYEKGKSVKLRLFFS-ESYCSKWKLFSDSK 486

Query: 488 LQLS--TKVGEDSFCLEKESD-TSIVVKKCICLDAESCMDDPQSQWFKLV 524
           +QLS  TK G  S CL+ +++  +IV   C CL   S   DP+SQWFKLV
Sbjct: 487 MQLSSITKNG-FSVCLDVDTENNNIVTNSCKCLRGNSSC-DPRSQWFKLV 522

[Close]

BLAST of CmoCh02G014500 vs. TAIR 10
Match: AT3G26140.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 383.3 bits (983), Expect = 3.2e-106
Identity = 195/508 (38.39%), Postives = 290/508 (57.09%), Query Frame = 0


Query: 23  PLSTSGRWIIDARTGDRVKLSCVNLVSHAQSMVAQGLDKRPLKELASEITSRQFNCVRLT 82
           PLST+ RWIID + G RVKL+CVN  SH Q +VA+GL K+ + +LA +I +  FNCVR T
Sbjct: 4   PLSTNSRWIIDEK-GQRVKLACVNWPSHLQPVVAEGLSKQSVDDLAKKIMAMGFNCVRFT 63

Query: 83  WSVHMFTRYAYE---CVGDVLDGLDIADVKSGVAKHNPEILKMTIAKAFQMVINCLGSEG 142
           W + + T         V      L + D  SG    NP ++ + + +A++ V+  LG+  
Sbjct: 64  WPLDLATNETLANNVTVRQSFQSLGLNDDISGFETKNPSMIDLPLIEAYKKVVAKLGNNN 123

Query: 143 IMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNEWLQGLSFVAAQFTCNPHVVGMSLRNE 202
           +MVILDNH+++P WCC  +DGNGFFGD  F+P  W+ GL+ +A  F    +VVGMSLRNE
Sbjct: 124 VMVILDNHVTKPGWCCGYNDGNGFFGDTFFDPTTWIAGLTKIAMTFKGATNVVGMSLRNE 183

Query: 203 LRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIISGLNYDNDLSQLKKRPLGYTFQNKVVL 262
           LRGP  N D W  YM++G++ +H  NPN+L+I+SGL+YD DLS ++ R +  TF  K+V 
Sbjct: 184 LRGPKQNVDDWFKYMQQGAEAVHEANPNVLVILSGLSYDTDLSFVRSRHVNLTFTRKLVF 243

Query: 263 EAHLYSFSGDNESKFVKKPLNIICNEIMEKFEREAGFVVDMKNPYPLFISEFGYDQSGGN 322
           E H YSF+  N + +  K  N  C EI++  E   GF +     +P+F+SEFG D  G N
Sbjct: 244 ELHRYSFT--NTNTWSSKNPNEACGEILKSIENGGGFNL---RDFPVFLSEFGIDLRGKN 303

Query: 323 EAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQGHEDPDESFGVMDHSWTKDRNPKLKQ 382
             +NR++ C L   AE D+DW++W  QGSY  R+G     E +G++D  W + R+    Q
Sbjct: 304 VNDNRYIGCILGWAAENDVDWSIWTLQGSYYLREGVVGMSEFYGILDSDWVRVRSQSFLQ 363

Query: 383 MLQLIKRVNQDLNSKAPMSYIILHPVSGQCVKSD--GKGGIELGDCATPTQWNHSGDASP 442
            L LI    Q   S++ +  ++ HP++G C+         + LG C     W+++   + 
Sbjct: 364 RLSLILSPLQGPGSQSKVYNLVFHPLTGLCMLQSILDPTKVTLGLCNESQPWSYT-PQNT 423

Query: 443 MKLLSNGQCLKSAG-DGKSPIVSTDCSGDEYN-WTVASKAKLQLSTKVGEDSFCLEKESD 502
           + L     CL+S G +    +  T CS    + W   S + + L+ K   +S CL+ +  
Sbjct: 424 LTLKDKSLCLESTGPNAPVKLSETSCSSPNLSEWETISASNMLLAAKSTNNSLCLDVDET 483

Query: 503 TSIVVKKCICLDAESCMDDPQSQWFKLV 524
            +++   C C+  E    DP SQWFK+V
Sbjct: 484 NNLMASNCKCVKGEDSSCDPISQWFKIV 504

[Close]

BLAST of CmoCh02G014500 vs. TAIR 10
Match: AT1G13130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 376.3 bits (965), Expect = 4.0e-104
Identity = 197/514 (38.33%), Postives = 292/514 (56.81%), Query Frame = 0


Query: 19  SQSLPLSTSGRWIIDARTGDRVKLSCVNLVSHAQSMVAQGLDKRPLKELASEITSRQFNC 78
           + S PLSTS RWI+D   G RVKL C N  SH Q +VA+GL K+P+  +A +I    FNC
Sbjct: 30  NMSYPLSTSSRWIVD-ENGLRVKLVCANWPSHLQPVVAEGLSKQPVDAVAKKIVEMGFNC 89

Query: 79  VRLTWSVHMFTRYAYE---CVGDVLDGLDIADVKSGVAKHNPEILKMTIAKAFQMVINCL 138
           VRLTW + + T         V      L + D   G   +NP I+ + + +A++ V+  L
Sbjct: 90  VRLTWPLDLMTNETLANNVTVRQSFQSLGLNDDIVGFQTNNPSIIDLPLIEAYKTVVTTL 149

Query: 139 GSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNEWLQGLSFVAAQFTCNPHVVGMS 198
           G+  +MVILDNH+++P WCC+ DDGNGFFGD+ F+P  W+  L  +AA F    +VVGMS
Sbjct: 150 GNNDVMVILDNHLTKPGWCCANDDGNGFFGDQFFDPTVWVAALKKMAATFNGVSNVVGMS 209

Query: 199 LRNELRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIISGLNYDNDLSQLKKRPLGYTFQN 258
           LRNELRGP  N + W  YM++G++ +H  N  +L+I+SGL++D DLS ++ RP+  +F  
Sbjct: 210 LRNELRGPKQNVNDWFKYMQQGAEAVHSANNKVLVILSGLSFDADLSFVRSRPVKLSFTG 269

Query: 259 KVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFEREAGFVVDMKNPYPLFISEFGYDQ 318
           K+V E H YSFS D  S     P N IC  ++ +     G++  +   +PLF+SEFG D+
Sbjct: 270 KLVFELHWYSFS-DGNSWAANNP-NDICGRVLNRIGNGGGYL--LNQGFPLFLSEFGIDE 329

Query: 319 SGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQGHEDPDESFGVMDHSWTKDRNP 378
            G N  +NR+  C     AE D+DW+LWA  GSY  RQG    +E +GV+D  W   RN 
Sbjct: 330 RGVNTNDNRYFGCLTGWAAENDVDWSLWALTGSYYLRQGKVGMNEYYGVLDSDWISVRNS 389

Query: 379 KLKQMLQLIKRVNQDLNSKAPMSYIILHPVSGQCV--KSDGKGGIELGDCATPTQWNHSG 438
              Q +  ++   Q    +     ++ HP++G C+    D    + LG C +   W+++ 
Sbjct: 390 SFLQKISFLQSPLQGPGPRTDAYNLVFHPLTGLCIVRSLDDPKMLTLGPCNSSEPWSYTK 449

Query: 439 DASPMKLLSNGQCLKSAGDGKSPI--VSTDCSGDEYNWTVASKAKLQL-STKVGEDSFCL 498
            A  +++     CL+S G  K+P+    T CS     W   S +++ L ST   + S CL
Sbjct: 450 KA--LRIKDQQLCLQSNGP-KNPVTMTRTSCSTSGSKWQTISASRMHLASTTSNKTSLCL 509

Query: 499 EKESDTSIVVKKCICLDAE-SCMDDPQSQWFKLV 524
           + ++  ++V   C CL  + SC  +P SQWFK++
Sbjct: 510 DVDTANNVVANACKCLSKDKSC--EPMSQWFKII 533

[Close]

BLAST of CmoCh02G014500 vs. TAIR 10
Match: AT5G17500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 353.2 bits (905), Expect = 3.6e-97
Identity = 200/526 (38.02%), Postives = 289/526 (54.94%), Query Frame = 0


Query: 5   LSLISLILFSLFAFSQSLPLSTSGRWIIDARTGDRVKLSCVNLVSHAQSMVAQGLDKRPL 64
           LSLISL L      +   PL T  RWI++ + G RVKL+C N  SH + +VA+GL  +P+
Sbjct: 15  LSLISLTL------ATDYPLFTKSRWIVNNK-GHRVKLACANWPSHLKPVVAEGLSSQPM 74

Query: 65  KELASEITSRQFNCVRLTWSVHMF---TRYAYECVGDVLDGLDIADVKSGVAKHNPEILK 124
             ++ +I    FNCVRLTW + +    T      V    +   +     G+  HNP I+ 
Sbjct: 75  DSISKKIKDMGFNCVRLTWPLELMINDTLAFNVTVKQSFERYGLDHELQGIYTHNPYIVN 134

Query: 125 MTIAKAFQMVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNEWLQGLSFV 184
             +   FQ V+  LG   +MVILDNH + P WCCS DD + FFGD  FNP+ W+ GL  +
Sbjct: 135 TPLINVFQAVVYSLGRHDVMVILDNHKTVPGWCCSNDDPDAFFGDPKFNPDLWMLGLKKM 194

Query: 185 AAQFTCNPHVVGMSLRNELRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIISGLNYDNDL 244
           A  F    +VVGMSLRNELRG    +  W  YM++G++ +H  NPN+L+I+SGLN+D DL
Sbjct: 195 ATIFMNVKNVVGMSLRNELRGYNHTSKDWYKYMQKGAEAVHTSNPNVLVILSGLNFDADL 254

Query: 245 SQLKKRPLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFEREAGFVVDMK 304
           S LK RP+  +F+ K+VLE H YSF+ D   ++    +N  C+++  K  R  GFV+D  
Sbjct: 255 SFLKDRPVNLSFKKKLVLELHWYSFT-DGTGQWKSHNVNDFCSQMFSKERRTGGFVLD-- 314

Query: 305 NPYPLFISEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQGHEDPDES 364
             +PLF+SEFG DQ GG+   NR+M+C LA  AEKD+DWA+WA  G Y +R+G     E+
Sbjct: 315 QGFPLFLSEFGTDQRGGDLEGNRYMNCMLAWAAEKDLDWAVWAVTGVYYFREGKRGVVEA 374

Query: 365 FGVMDHSWTKDRNPKLKQMLQLIKRVNQDLNSKAPMSYIILHPVSGQCVKSDG---KGGI 424
           +G++D +W    N    + L +I+  +     K      I HP++G C+       +  +
Sbjct: 375 YGMLDANWHNVHNYTYLRRLSVIQPPHTGPGVKHNHHKKIFHPLTGLCLVRKSHCHESEL 434

Query: 425 ELGDCATPTQWNHSGDASPMKLLSNGQCLK-SAGDGKSPIVSTDCSGDEYNWTVASKAKL 484
            LG C     W++S          +  CL+     GKS  +   C+  E      S  K+
Sbjct: 435 TLGPCTKDEPWSYSHGGILEIRRGHKSCLEGETAVGKSVKLGRICTKIEQ----ISATKM 494

Query: 485 QLSTKVGEDSF-CLEKESDTSIVVKKCICLDAESCMDDPQSQWFKL 523
            LS    + S  CL+ +SD ++V   C CL  ++   +P SQWFK+
Sbjct: 495 HLSFNTSDGSLVCLDVDSDNNVVANSCNCLTGDTTC-EPASQWFKI 525

[Close]

BLAST of CmoCh02G014500 vs. TAIR 10
Match: AT5G16700.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 318.2 bits (814), Expect = 1.3e-86
Identity = 195/529 (36.86%), Postives = 276/529 (52.17%), Query Frame = 0


Query: 7   LISLILFSLFAFSQSLPLSTSGRWIIDARTGDRVKLSCVNLVSHAQSMVAQGLDKRPLKE 66
           L  L + S    + S PLST  RWI+D + G RVKL+CVN  +H Q  VA+GL K+PL  
Sbjct: 9   LFFLFISSTSKLTTSYPLSTKSRWIVDEK-GQRVKLACVNWPAHLQPTVAEGLSKQPLDS 68

Query: 67  LASEITSRQFNCVRLTWSVHMFTRYAYE---CVGDVLDGLDIADVKSGVAKHNPEILKMT 126
           ++ +I S  FNCVRLTW + + T         V    + L + +   G+  HNP++L + 
Sbjct: 69  ISKKIVSMGFNCVRLTWPLDLVTNDTLALKVTVKQSFESLKLFEDVLGIQTHNPKLLHLP 128

Query: 127 IAKAFQMVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNEWLQGLSFVAA 186
           +  AFQ V++ LG  G+MVILDNH++ P WCC  +D + FFG  +F+P  W +GL  +A 
Sbjct: 129 LFNAFQEVVSNLGENGVMVILDNHLTTPGWCCGDNDLDAFFGYPHFDPLVWAKGLRKMAT 188

Query: 187 QFTCNPHVVGMSLRNELRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIISGLNYDNDLSQ 246
            F    HV+GMSLRNE RG     D W  +M +G++ +H  NP LL+I+SG+++D +LS 
Sbjct: 189 LFRNFTHVIGMSLRNEPRGARDYPDLWFRHMPQGAEAVHAANPKLLVILSGIDFDTNLSF 248

Query: 247 LKKRPLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFEREAGFVVDMKNP 306
           L+ R +  +F +K+V E H YSFS D    + K   N  C +I+EK     GF+  +   
Sbjct: 249 LRDRSVNVSFTDKLVFELHWYSFS-DGRDSWRKHNSNDFCVKIIEKVTHNGGFL--LGRG 308

Query: 307 YPLFISEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQGHEDPDESFG 366
           +PL +SEFG DQ GG+ + NR+M+C +A  AE D+DWA+WA  G Y  R G         
Sbjct: 309 FPLILSEFGTDQRGGDMSGNRYMNCLVAWAAENDLDWAVWALTGDYYLRTG--------- 368

Query: 367 VMDHSWTKDRNPKLKQMLQLIKRVNQDLNSKAPMSYIILHPVSGQCV---KSDGKGGIEL 426
                      P L+                 P   ++ HP +G CV    SD    + L
Sbjct: 369 -----------PGLR-----------------PNKNLLFHPSTGLCVTNNPSDNIPTLRL 428

Query: 427 GDCATPTQWNHSGDASPMKLLSNGQCLKS---AGDGKSPIVSTDCSGDEYNWTVASKAKL 486
           G C     W    + S   L  N  C+++    G      V T CS  +     A+K  L
Sbjct: 429 GPCPKSDPWTF--NPSEGILWINKMCVEAPNVVGQKVKLGVGTKCS--KLGQISATKMHL 488

Query: 487 QLSTKVGEDSFCLE-KESDTSIVVKKC--ICLDAESCMDDPQSQWFKLV 524
              T  G    CL+  E D S+V  +C  + +DA SC  DP SQWFK++
Sbjct: 489 SFKTSNGL-LLCLDVDERDNSVVANRCKFLTMDA-SC--DPASQWFKVL 488

The following BLAST results are available for this feature:

BLAST of CmoCh02G014500 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
C0HLA0	3.4e-116	40.89	Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1	[more]
P19487	6.5e-11	23.43	Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (stra...	[more]

BLAST of CmoCh02G014500 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GZR4	3.8e-288	100.00	Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111459103 P...	[more]
A0A6J1K7T8	7.4e-284	97.89	Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111491432 PE=...	[more]
A0A6J1H0Z1	3.1e-282	97.68	Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111459153 P...	[more]
A0A6J1H187	4.5e-281	97.05	Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111459155 P...	[more]
A0A6J1JQD4	1.8e-266	89.89	Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111487386 PE=...	[more]

BLAST of CmoCh02G014500 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6601845.1	2.1e-288	88.83	hypothetical protein SDJN03_07078, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022957626.1	7.9e-288	100.00	uncharacterized protein LOC111459103 [Cucurbita moschata]	[more]
XP_022996114.1	1.5e-283	97.89	uncharacterized protein LOC111491432 [Cucurbita maxima]	[more]
KAG6606063.1	4.9e-282	97.89	hypothetical protein SDJN03_03380, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022957688.1	6.4e-282	97.68	uncharacterized protein LOC111459153 [Cucurbita moschata]	[more]

BLAST of CmoCh02G014500 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G26130.1	1.5e-111	42.64	Cellulase (glycosyl hydrolase family 5) protein	[more]
AT3G26140.1	3.2e-106	38.39	Cellulase (glycosyl hydrolase family 5) protein	[more]
AT1G13130.1	4.0e-104	38.33	Cellulase (glycosyl hydrolase family 5) protein	[more]
AT5G17500.1	3.6e-97	38.02	Glycosyl hydrolase superfamily protein	[more]
AT5G16700.1	1.3e-86	36.86	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 24..385 e-value: 1.8E-66 score: 226.7
None	No IPR available	GENE3D	2.80.10.50		coord: 400..522 e-value: 5.0E-5 score: 25.3
None	No IPR available	PANTHER	PTHR31263	CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)	coord: 6..524
IPR001547	Glycoside hydrolase, family 5	PFAM	PF00150	Cellulase	coord: 63..345 e-value: 1.6E-22 score: 80.3
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 22..384

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh02G014500.1	CmoCh02G014500.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071704	organic substance metabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0016985	mannan endo-1,4-beta-mannosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds