CmoCh02G008260 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh02G008260
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionTHO complex subunit 4A-like
LocationCmo_Chr02: 5038451 .. 5040207 (-)
RNA-Seq ExpressionCmoCh02G008260
SyntenyCmoCh02G008260
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TAGAAACCACGACGCGGTCCTCTGAAGTCTTTATATGTTCAGAGAAAAATCGGCATCAGCCCTCAATTTTCTCCCAAACCCTTCACTCTCGACGAAACTGCGAAACCCTAATTTTCTCCTCTCTCTCTCTCTCTTACTCCCTCTTTCGACTGCTCCTTCAGTGTTTCTGTTCGATTTCTCTTTGAACATGGCCGCTCCTTTGGATATGAGTCTCGATGATATCATAAAGAACAACAAAAAATCCAGATCCGGAATTTCTAGAGGTCGCGGTAGAGGGTCCGGACCGGGTCCTGTCCGTCGAATCCCCAATCGCGCCGCTAATCGCACACCTTATTCCGCTCCCAAGGTATAGCTTTGAGGATTAGTTTCTTGCTGCCGGTTTTCCTCTCTCGAATTCTGGGTTGAAGTTTTGATTTGTGATTTTGGTGTTAGGCGCCGGAGACGACCTGGCAGCATGATATGTTTGTCGATCAGGGCGCGGGTTTCCCCGTGCAAGCTGGACGTGCCTCTAGCATTCAGACTGGAACTAAGCTCTACATATCTAATTTAGATTATGGTGTATCTAACGAAGATATTAAGGTATTTATACTTCTTGCTGATAGGTTAAATGATAGGATAGGTTTCCTTTTTTTGTTTTTGTTTTTTGCTGAAAGTGATGATTTTCGCAGGAACTTTTTTCTGAGGTCGGAGATATGAAACGCTACGGTATCCATTATGACAAAAGTGGCAGATCCAAGGTATTCTACGAGGCTTGGCGCAATACTGGGTTTAGACTTCTAGTGAATTTTGTATCTTGGTCGAAGTTTAGAAATTCTCTTTTCCATAGGGAACGGCGGAAGTAGTTTTCTCACGACGAGGAGATGCTGCTGCGGCTGTCAAGAAGTACAACAACGTGCAGCTAGATGGAAAACCGATGAAGATAGAGATCGTTGGAACTAATATTGCCACTCATGCTGTTGGTCCTCCCGCTGCTGTGAACCCTTTCGAAAATTTCAATGGGGCTCCTAGAAGGTATTTATCAGTATACGATATGTCTGCTCTGATCCTTGGTTAGGTTTATAATTGGATGCAACAATTGCCTGCAACTTGCTTTTTCAAAACATATTTCGAATTTCAATTCGTGTTTGAAATTCAGCTAATTTGCTATAAGCTACTTCTATTTTTTGGAATCCTATACATATAGATATAAGTTTGATAAATATTAGAGTTTTGTTTTCCTTGTATTAATGATTTTAATTTCTGTTTATAAAGAACTCTTACTGCAGTTTGCTTATAGCTTTTGGAGTAGTGAACTTAATGTTTATTTTAGGTTGTGATATGCTTGTAATACTCATTGTCTAGAATTATAATTTTTTTTTATCGGGTATTTTTATTGCAGAACAGTTTTCCATGTGTTTTTTTACTTGAATCAATTTTTTAGACCTTTGCTATATATGTACTTATGTGAGCCATCGATGTTCTGTTGTATGCACTTGTATCTGAATCTCTTTATTATTTTTAGGCAAGTTTGTTTCGCTTTGGTGCTATTCATTTTATAAGTAAGGCTACCATCCTCTCAGTCATTCTACATCAAATTGACATTTTTTTAACTGTTAAACAGGCAACAAGGTAGGGGTGGTCTGCCATCACGTCAACGTGGTGGTCGTGGTTCTGGAAGGGGGCGTGGGCGGGGAAGAGGCCCAAGTGAAAAAGTATCTGCTGAAGATCTTGATGCCGACTTGGAAAAGTATCATGCTGAGTCGATGCAGATAAATTAG

mRNA sequence

TAGAAACCACGACGCGGTCCTCTGAAGTCTTTATATGTTCAGAGAAAAATCGGCATCAGCCCTCAATTTTCTCCCAAACCCTTCACTCTCGACGAAACTGCGAAACCCTAATTTTCTCCTCTCTCTCTCTCTCTTACTCCCTCTTTCGACTGCTCCTTCAGTGTTTCTGTTCGATTTCTCTTTGAACATGGCCGCTCCTTTGGATATGAGTCTCGATGATATCATAAAGAACAACAAAAAATCCAGATCCGGAATTTCTAGAGGTCGCGGTAGAGGGTCCGGACCGGGTCCTGTCCGTCGAATCCCCAATCGCGCCGCTAATCGCACACCTTATTCCGCTCCCAAGGCGCCGGAGACGACCTGGCAGCATGATATGTTTGTCGATCAGGGCGCGGGTTTCCCCGTGCAAGCTGGACGTGCCTCTAGCATTCAGACTGGAACTAAGCTCTACATATCTAATTTAGATTATGGTGTATCTAACGAAGATATTAAGGAACTTTTTTCTGAGGTCGGAGATATGAAACGCTACGGTATCCATTATGACAAAAGTGGCAGATCCAAGGGAACGGCGGAAGTAGTTTTCTCACGACGAGGAGATGCTGCTGCGGCTGTCAAGAAGTACAACAACGTGCAGCTAGATGGAAAACCGATGAAGATAGAGATCGTTGGAACTAATATTGCCACTCATGCTGTTGGTCCTCCCGCTGCTGTGAACCCTTTCGAAAATTTCAATGGGGCTCCTAGAAGAACAGTTTTCCATGTGTTTTTTTACTTGAATCAATTTTTTAGACCTTTGCTATATATGTACTTATGCAAGTTTGTTTCGCTTTGGTGCTATTCATTTTATAAGCAACAAGGTAGGGGTGGTCTGCCATCACGTCAACGTGGTGGTCGTGGTTCTGGAAGGGGGCGTGGGCGGGGAAGAGGCCCAAGTGAAAAAGTATCTGCTGAAGATCTTGATGCCGACTTGGAAAAGTATCATGCTGAGTCGATGCAGATAAATTAG

Coding sequence (CDS)

ATGTTCAGAGAAAAATCGGCATCAGCCCTCAATTTTCTCCCAAACCCTTCACTCTCGACGAAACTGCGAAACCCTAATTTTCTCCTCTCTCTCTCTCTCTTACTCCCTCTTTCGACTGCTCCTTCAGTGTTTCTGTTCGATTTCTCTTTGAACATGGCCGCTCCTTTGGATATGAGTCTCGATGATATCATAAAGAACAACAAAAAATCCAGATCCGGAATTTCTAGAGGTCGCGGTAGAGGGTCCGGACCGGGTCCTGTCCGTCGAATCCCCAATCGCGCCGCTAATCGCACACCTTATTCCGCTCCCAAGGCGCCGGAGACGACCTGGCAGCATGATATGTTTGTCGATCAGGGCGCGGGTTTCCCCGTGCAAGCTGGACGTGCCTCTAGCATTCAGACTGGAACTAAGCTCTACATATCTAATTTAGATTATGGTGTATCTAACGAAGATATTAAGGAACTTTTTTCTGAGGTCGGAGATATGAAACGCTACGGTATCCATTATGACAAAAGTGGCAGATCCAAGGGAACGGCGGAAGTAGTTTTCTCACGACGAGGAGATGCTGCTGCGGCTGTCAAGAAGTACAACAACGTGCAGCTAGATGGAAAACCGATGAAGATAGAGATCGTTGGAACTAATATTGCCACTCATGCTGTTGGTCCTCCCGCTGCTGTGAACCCTTTCGAAAATTTCAATGGGGCTCCTAGAAGAACAGTTTTCCATGTGTTTTTTTACTTGAATCAATTTTTTAGACCTTTGCTATATATGTACTTATGCAAGTTTGTTTCGCTTTGGTGCTATTCATTTTATAAGCAACAAGGTAGGGGTGGTCTGCCATCACGTCAACGTGGTGGTCGTGGTTCTGGAAGGGGGCGTGGGCGGGGAAGAGGCCCAAGTGAAAAAGTATCTGCTGAAGATCTTGATGCCGACTTGGAAAAGTATCATGCTGAGTCGATGCAGATAAATTAG

Protein sequence

MFREKSASALNFLPNPSLSTKLRNPNFLLSLSLLLPLSTAPSVFLFDFSLNMAAPLDMSLDDIIKNNKKSRSGISRGRGRGSGPGPVRRIPNRAANRTPYSAPKAPETTWQHDMFVDQGAGFPVQAGRASSIQTGTKLYISNLDYGVSNEDIKELFSEVGDMKRYGIHYDKSGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKIEIVGTNIATHAVGPPAAVNPFENFNGAPRRTVFHVFFYLNQFFRPLLYMYLCKFVSLWCYSFYKQQGRGGLPSRQRGGRGSGRGRGRGRGPSEKVSAEDLDADLEKYHAESMQIN
Homology
BLAST of CmoCh02G008260 vs. ExPASy Swiss-Prot
Match: Q8L773 (THO complex subunit 4A OS=Arabidopsis thaliana OX=3702 GN=ALY1 PE=1 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 1.8e-67
Identity = 158/284 (55.63%), Postives = 189/284 (66.55%), Query Frame = 0

Query: 52  MAAPLDMSLDDIIKNNKKSRSGI--SRGRGRGSGPGPVRR-IPNRAANRT-PYSAPKAPE 111
           M+  LDMSLDD+I  N+KSR G   +RG G GSGPGP RR  PNR + R+ PY + KAPE
Sbjct: 1   MSTGLDMSLDDMIAKNRKSRGGAGPARGTGSGSGPGPTRRNNPNRKSTRSAPYQSAKAPE 60

Query: 112 TTWQHDMFVDQGAGFPVQAGRASS-IQTGTKLYISNLDYGVSNEDIKELFSEVGDMKRYG 171
           +TW HDMF D+      ++GR+S+ I+TGTKLYISNLDYGV NEDIKELF+EVG++KRY 
Sbjct: 61  STWGHDMFSDRSEDH--RSGRSSAGIETGTKLYISNLDYGVMNEDIKELFAEVGELKRYT 120

Query: 172 IHYDKSGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKIEIVGTNIATHAVGPPAAV 231
           +H+D+SGRSKGTAEVV+SRRGDA AAVKKYN+VQLDGKPMKIEIVGTN+ T A   P+  
Sbjct: 121 VHFDRSGRSKGTAEVVYSRRGDALAAVKKYNDVQLDGKPMKIEIVGTNLQTAAA--PSGR 180

Query: 232 NPFENFNGAPRRTVFHVFFYLNQFFRPLLYMYLCKFVSLWCYSFYKQQGRGGLPSRQRGG 291
               N NGAP R                                   QGRGG   +QRGG
Sbjct: 181 PANGNSNGAPWR---------------------------------GGQGRGG---QQRGG 240

Query: 292 -------RGSGRGRGRGRGPSEKVSAEDLDADLEKYHAESMQIN 324
                   G GRGR  G+GP+EK+SAEDLDADL+KYH+  M+ N
Sbjct: 241 GRGGGGRGGGGRGRRPGKGPAEKISAEDLDADLDKYHSGDMETN 244

BLAST of CmoCh02G008260 vs. ExPASy Swiss-Prot
Match: Q8L719 (THO complex subunit 4B OS=Arabidopsis thaliana OX=3702 GN=ALY2 PE=1 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 5.2e-59
Identity = 161/317 (50.79%), Postives = 183/317 (57.73%), Query Frame = 0

Query: 52  MAAPLDMSLDDIIKNNKK-----SRSGISRGR------GRGSGPGPVRRIPNRAANRT-P 111
           M+  LDMSLDDIIK+N+K      R GI  G       G GS  GP RR  NR   RT P
Sbjct: 1   MSGGLDMSLDDIIKSNRKPTGSRGRGGIGGGNNTGGRGGSGSNSGPSRRFANRVGARTAP 60

Query: 112 YSAP----KAPETTWQHDMFVDQ-------GAGFPVQAGRASSIQTGTKLYISNLDYGVS 171
           YS P    +A +  WQ+D+F          G       G  SSI+TGTKLYISNLDYGVS
Sbjct: 61  YSRPIQQQQAHDAMWQNDVFATDASVAAAFGHHQTAVVGGGSSIETGTKLYISNLDYGVS 120

Query: 172 NEDIKELFSEVGDMKRYGIHYDKSGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKI 231
           NEDIKELFSEVGD+KRYGIHYD+SGRSKGTAEVVFSRRGDA AAVK+YNNVQLDGK MKI
Sbjct: 121 NEDIKELFSEVGDLKRYGIHYDRSGRSKGTAEVVFSRRGDALAAVKRYNNVQLDGKLMKI 180

Query: 232 EIVGTNIATHAVGPPAAVN-PF----------ENFNGAPRRTVFHVFFYLNQFFRPLLYM 291
           EIVGTN++  A+   A    PF          ENFNG      F+  F  N         
Sbjct: 181 EIVGTNLSAPALPILATAQIPFPTNGILGNFNENFNGN-----FNGNFNGN--------- 240

Query: 292 YLCKFVSLWCYSFYKQQGRGGLPSRQRGG-------RGSGRGRGR------GRGPSEKVS 322
                        ++ +GRGG   R RGG       RG    RGR      GRG  E VS
Sbjct: 241 -------------FRGRGRGGFMGRPRGGGFGGGNFRGGRGARGRGGRGSGGRGRDENVS 290

BLAST of CmoCh02G008260 vs. ExPASy Swiss-Prot
Match: Q6NQ72 (THO complex subunit 4D OS=Arabidopsis thaliana OX=3702 GN=ALY4 PE=1 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 4.0e-43
Identity = 130/306 (42.48%), Postives = 169/306 (55.23%), Query Frame = 0

Query: 52  MAAPLDMSLDDIIKNNKKSRS---GISRGRGR-----GSGPGPVRRIPNRAANRTPYS-- 111
           M+  L+M+LD+I+K  K +RS   GISRGRGR     G G GP RR P  A N  P S  
Sbjct: 1   MSGALNMTLDEIVKRGKTARSGGRGISRGRGRGRGGGGRGAGPARRGP-LAVNARPSSFT 60

Query: 112 ----APKAPETTWQHDMFVDQGAGFPVQAGRASSIQTGTKLYISNLDYGVSNEDIKELFS 171
                 +     WQ  +F D      ++A  AS ++ GT+L+++NLD GV+NEDI+ELFS
Sbjct: 61  INKPVRRVRSLPWQSGLFED-----GLRAAGASGVEVGTRLHVTNLDQGVTNEDIRELFS 120

Query: 172 EVGDMKRYGIHYDKSGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKIEIVGTNIAT 231
           E+G+++RY IHYDK+GR  GTAEVV+ RR DA  A+KKYNNV LDG+PM++EI+G N ++
Sbjct: 121 EIGEVERYAIHYDKNGRPSGTAEVVYPRRSDAFQALKKYNNVLLDGRPMRLEILGGNNSS 180

Query: 232 HAVGPPAAVNPFENFNGAPRRTVFHVFFYLNQFF---------------RPLLYMYLCKF 291
            A             NG  +RTV      + Q                  P +   L   
Sbjct: 181 EAPLSGRVNVNVTGLNGRLKRTV-----VIQQGGGGRGRVRGGRGGRGPAPTVSRRL--- 240

Query: 292 VSLWCYSFYKQQ------GRGGLPSRQR--GGRGSGRGRGRGRGPSEKVSAEDLDADLEK 321
                   + QQ      GRGG  +R R  GGRG G GRG G+ P EK SA DLD DLE 
Sbjct: 241 ------PIHNQQGGGMRGGRGGFRARGRGNGGRGRGGGRGNGKKPVEK-SAADLDKDLES 285

BLAST of CmoCh02G008260 vs. ExPASy Swiss-Prot
Match: Q94EH8 (THO complex subunit 4C OS=Arabidopsis thaliana OX=3702 GN=ALY3 PE=1 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 5.9e-39
Identity = 127/310 (40.97%), Postives = 171/310 (55.16%), Query Frame = 0

Query: 52  MAAPLDMSLDDIIKNNKKSRS--------GISR--GRGRGS---------GPGPVRRIPN 111
           M+  L+M+LD+I+K +K  RS        G+SR  GRGRG          G GPVRR P 
Sbjct: 1   MSDALNMTLDEIVKKSKSERSAAARSGGKGVSRKSGRGRGGPNGVVGGGRGGGPVRRGP- 60

Query: 112 RAANRTPYS-------APKAPETTW--QHDMFVDQGAGFPVQAGRASSIQTGTKLYISNL 171
            A N  P S       A +     W  Q+D++ +      ++A   S ++ GT +YI+NL
Sbjct: 61  LAVNTRPSSSFSINKLARRKRSLPWQNQNDLYEE-----TLRAVGVSGVEVGTTVYITNL 120

Query: 172 DYGVSNEDIKELFSEVGDMKRYGIHYDKSGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDG 231
           D GV+NEDI+EL++E+G++KRY IHYDK+GR  G+AEVV+ RR DA  A++KYNNV LDG
Sbjct: 121 DQGVTNEDIRELYAEIGELKRYAIHYDKNGRPSGSAEVVYMRRSDAIQAMRKYNNVLLDG 180

Query: 232 KPMKIEIVGTNIATHAVGPPAAVNPFENFNGAPRRTVFHVFFYLNQFFRPLLYMYLCKFV 291
           +PMK+EI+G N  +    P AA       NG  +R+VF     + Q  R           
Sbjct: 181 RPMKLEILGGNTES---APVAARVNVTGLNGRMKRSVF-----IGQGVRGGRVGRGRGSG 240

Query: 292 SLWCYSFYKQQGRGGLPSRQRGGRGSGRGRGRGRG----------PSEKVSAEDLDADLE 324
                   +Q  +GG+ + + G RG GRG G GRG          P EK SA DLD DLE
Sbjct: 241 PSGRRLPLQQNQQGGVTAGRGGFRGRGRGNGGGRGNKSGGRGGKKPVEK-SAADLDKDLE 295

BLAST of CmoCh02G008260 vs. ExPASy Swiss-Prot
Match: Q3T0I4 (THO complex subunit 4 OS=Bos taurus OX=9913 GN=ALYREF PE=2 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 1.5e-37
Identity = 118/296 (39.86%), Postives = 149/296 (50.34%), Query Frame = 0

Query: 52  MAAPLDMSLDDIIKNNKKSRSGISRGRGRG-------------------SGPGPVRRIPN 111
           MA  +DMSLDDIIK N+  R G   GRGRG                    G GP+R  P 
Sbjct: 1   MADKMDMSLDDIIKLNRSQRGGRGGGRGRGRAGSQGGRGGGAQAAARVNRGGGPIRNRPA 60

Query: 112 RA--------ANR-TPYSAPKAPETTWQHDMFVDQGAGFPVQAGRASSIQTGTKLYISNL 171
            A         NR  PYS PK     WQHD+F    +GF    G  + ++TG KL +SNL
Sbjct: 61  IARGAAGGGGRNRPAPYSRPKQLPDKWQHDLF---DSGF----GGGAGVETGGKLLVSNL 120

Query: 172 DYGVSNEDIKELFSEVGDMKRYGIHYDKSGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDG 231
           D+GVS+ DI+ELF+E G +K+  +HYD+SGRS GTA+V F R+ DA  A+K+YN V LDG
Sbjct: 121 DFGVSDADIQELFAEFGTLKKAAVHYDRSGRSLGTADVHFERKADALKAMKQYNGVPLDG 180

Query: 232 KPMKIEIVGTNIATHAVGPPAAVNPFENFNGAPRRTVFHVFFYLNQFFRPLLYMYLCKFV 291
           +PM I++V + I T    P  +VN      G   R                         
Sbjct: 181 RPMNIQLVTSQIDTQR-RPAQSVN-----RGGMTRN------------------------ 240

Query: 292 SLWCYSFYKQQGRGGLPSRQRGGRGSGRGRGRGRGPSEK--VSAEDLDADLEKYHA 318
                      G GG    +RG RG  RGRGRG G S K  +SAE+LDA L+ Y+A
Sbjct: 241 -------RGSGGFGGGGGTRRGTRGGSRGRGRGTGRSSKQQLSAEELDAQLDAYNA 252

BLAST of CmoCh02G008260 vs. ExPASy TrEMBL
Match: A0A6J1G721 (THO complex subunit 4A-like OS=Cucurbita moschata OX=3662 GN=LOC111451345 PE=4 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 8.1e-124
Identity = 238/272 (87.50%), Postives = 238/272 (87.50%), Query Frame = 0

Query: 52  MAAPLDMSLDDIIKNNKKSRSGISRGRGRGSGPGPVRRIPNRAANRTPYSAPKAPETTWQ 111
           MAAPLDMSLDDIIKNNKKSRSGISRGRGRGSGPGPVRRIPNRAANRTPYSAPKAPETTWQ
Sbjct: 1   MAAPLDMSLDDIIKNNKKSRSGISRGRGRGSGPGPVRRIPNRAANRTPYSAPKAPETTWQ 60

Query: 112 HDMFVDQGAGFPVQAGRASSIQTGTKLYISNLDYGVSNEDIKELFSEVGDMKRYGIHYDK 171
           HDMFVDQGAGFPVQAGRASSIQTGTKLYISNLDYGVSNEDIKELFSEVGDMKRYGIHYDK
Sbjct: 61  HDMFVDQGAGFPVQAGRASSIQTGTKLYISNLDYGVSNEDIKELFSEVGDMKRYGIHYDK 120

Query: 172 SGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKIEIVGTNIATHAVGPPAAVNPFEN 231
           SGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKIEIVGTNIATHAVGPPAAVNPFEN
Sbjct: 121 SGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKIEIVGTNIATHAVGPPAAVNPFEN 180

Query: 232 FNGAPRRTVFHVFFYLNQFFRPLLYMYLCKFVSLWCYSFYKQQGRGGLPSRQRGGRGSGR 291
           FNGAPRR                                  QQGRGGLPSRQRGGRGSGR
Sbjct: 181 FNGAPRR----------------------------------QQGRGGLPSRQRGGRGSGR 238

Query: 292 GRGRGRGPSEKVSAEDLDADLEKYHAESMQIN 324
           GRGRGRGPSEKVSAEDLDADLEKYHAESMQIN
Sbjct: 241 GRGRGRGPSEKVSAEDLDADLEKYHAESMQIN 238

BLAST of CmoCh02G008260 vs. ExPASy TrEMBL
Match: A0A6J1L1M6 (THO complex subunit 4A-like OS=Cucurbita maxima OX=3661 GN=LOC111499593 PE=4 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 2.4e-123
Identity = 237/272 (87.13%), Postives = 237/272 (87.13%), Query Frame = 0

Query: 52  MAAPLDMSLDDIIKNNKKSRSGISRGRGRGSGPGPVRRIPNRAANRTPYSAPKAPETTWQ 111
           MAAPLDMSLDDIIKNNKKSRSGISRGRGRGSGPGPVRRIPNRAANRTPYSAPKAPETTWQ
Sbjct: 1   MAAPLDMSLDDIIKNNKKSRSGISRGRGRGSGPGPVRRIPNRAANRTPYSAPKAPETTWQ 60

Query: 112 HDMFVDQGAGFPVQAGRASSIQTGTKLYISNLDYGVSNEDIKELFSEVGDMKRYGIHYDK 171
           HDMFVDQGAGFPVQAGRASSIQTGTKLYISNLDYGVSNEDIKELFSEVGDMKRYGIHYDK
Sbjct: 61  HDMFVDQGAGFPVQAGRASSIQTGTKLYISNLDYGVSNEDIKELFSEVGDMKRYGIHYDK 120

Query: 172 SGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKIEIVGTNIATHAVGPPAAVNPFEN 231
           SGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKIEIVGTNIATHAVGPPAAVNPFEN
Sbjct: 121 SGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKIEIVGTNIATHAVGPPAAVNPFEN 180

Query: 232 FNGAPRRTVFHVFFYLNQFFRPLLYMYLCKFVSLWCYSFYKQQGRGGLPSRQRGGRGSGR 291
           FNG PRR                                  QQGRGGLPSRQRGGRGSGR
Sbjct: 181 FNGVPRR----------------------------------QQGRGGLPSRQRGGRGSGR 238

Query: 292 GRGRGRGPSEKVSAEDLDADLEKYHAESMQIN 324
           GRGRGRGPSEKVSAEDLDADLEKYHAESMQIN
Sbjct: 241 GRGRGRGPSEKVSAEDLDADLEKYHAESMQIN 238

BLAST of CmoCh02G008260 vs. ExPASy TrEMBL
Match: A0A0A0KBU2 (RRM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G193610 PE=4 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 7.4e-109
Identity = 218/272 (80.15%), Postives = 222/272 (81.62%), Query Frame = 0

Query: 52  MAAPLDMSLDDIIKNNKKSRSGISRGRGRGSGPGPVRRIPNRAANRTPYSAPKAPETTWQ 111
           MAAPLDMSLDDIIKNNKKSRSG SRGRGRGSGPGPVRR PNRAANRTPYSAPKAPETTWQ
Sbjct: 1   MAAPLDMSLDDIIKNNKKSRSGNSRGRGRGSGPGPVRRFPNRAANRTPYSAPKAPETTWQ 60

Query: 112 HDMFVDQGAGFPVQAGRASSIQTGTKLYISNLDYGVSNEDIKELFSEVGDMKRYGIHYDK 171
           HDMF D  +GF VQ GRAS+IQTGTKLYISNLDYGVSNEDIKELFSEVGDMKR+GIHYDK
Sbjct: 61  HDMFADPSSGFLVQGGRASAIQTGTKLYISNLDYGVSNEDIKELFSEVGDMKRHGIHYDK 120

Query: 172 SGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKIEIVGTNIATHAVGPPAAVNPFEN 231
           SGRSKGTAEVVFSRR DA AAVKKYNNVQLDGKPMKIEIVGTNI+T AVGP AAVNPFEN
Sbjct: 121 SGRSKGTAEVVFSRRLDAVAAVKKYNNVQLDGKPMKIEIVGTNISTPAVGPTAAVNPFEN 180

Query: 232 FNGAPRRTVFHVFFYLNQFFRPLLYMYLCKFVSLWCYSFYKQQGRGGLPSRQRGGRGSGR 291
            NGAPRR                                  QQGRGG PSRQR G G GR
Sbjct: 181 SNGAPRR----------------------------------QQGRGGPPSRQR-GHGFGR 237

Query: 292 GRGRGRGPSEKVSAEDLDADLEKYHAESMQIN 324
           GRGRGRGPSEKVSAEDLDADLEKYHAESMQIN
Sbjct: 241 GRGRGRGPSEKVSAEDLDADLEKYHAESMQIN 237

BLAST of CmoCh02G008260 vs. ExPASy TrEMBL
Match: A0A1S3BK70 (THO complex subunit 4A-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490589 PE=4 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 4.8e-108
Identity = 217/272 (79.78%), Postives = 222/272 (81.62%), Query Frame = 0

Query: 52  MAAPLDMSLDDIIKNNKKSRSGISRGRGRGSGPGPVRRIPNRAANRTPYSAPKAPETTWQ 111
           MAAPLDMSLDDIIKNNKKSRSG SRGRGRGSGPGPVRR PNRAA+RTPYSAPKAPETTWQ
Sbjct: 1   MAAPLDMSLDDIIKNNKKSRSGNSRGRGRGSGPGPVRRFPNRAASRTPYSAPKAPETTWQ 60

Query: 112 HDMFVDQGAGFPVQAGRASSIQTGTKLYISNLDYGVSNEDIKELFSEVGDMKRYGIHYDK 171
           HDMF D  +GF  Q GRAS+IQTGTKLYISNLDYGVSNEDIKELFSEVGDMKR+GIHYDK
Sbjct: 61  HDMFADPSSGFIGQGGRASAIQTGTKLYISNLDYGVSNEDIKELFSEVGDMKRHGIHYDK 120

Query: 172 SGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKIEIVGTNIATHAVGPPAAVNPFEN 231
           SGRSKGTAEVVFSRR DA AAVKKYNNVQLDGKPMKIEIVGTNI+T AVGP AAVNPFEN
Sbjct: 121 SGRSKGTAEVVFSRRLDAVAAVKKYNNVQLDGKPMKIEIVGTNISTPAVGPTAAVNPFEN 180

Query: 232 FNGAPRRTVFHVFFYLNQFFRPLLYMYLCKFVSLWCYSFYKQQGRGGLPSRQRGGRGSGR 291
            NGAPRR                                  QQGRGG PSRQR GRG GR
Sbjct: 181 SNGAPRR----------------------------------QQGRGGPPSRQR-GRGFGR 237

Query: 292 GRGRGRGPSEKVSAEDLDADLEKYHAESMQIN 324
           GRGRGRGPSEKVSAEDLDADLEKYHAESMQIN
Sbjct: 241 GRGRGRGPSEKVSAEDLDADLEKYHAESMQIN 237

BLAST of CmoCh02G008260 vs. ExPASy TrEMBL
Match: A0A6J1D4W8 (THO complex subunit 4A-like OS=Momordica charantia OX=3673 GN=LOC111017043 PE=4 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 1.7e-105
Identity = 216/277 (77.98%), Postives = 220/277 (79.42%), Query Frame = 0

Query: 52  MAAPLDMSLDDIIKNNKKSRSGISR-GRGRGSGPGPVRRIPNRAANRTPYSAPKAPETTW 111
           MAAPLDMSLDDIIKNNKKSRSG SR GRGR SGPGPVRR PNRAANRTPY+APKAPET W
Sbjct: 1   MAAPLDMSLDDIIKNNKKSRSGNSRGGRGRPSGPGPVRRFPNRAANRTPYAAPKAPETAW 60

Query: 112 QHDMFVDQGAGFPVQAGRASSIQTGTKLYISNLDYGVSNEDIKELFSEVGDMKRYGIHYD 171
           QHDMF+DQ +GF VQAGRAS+IQTGTKLYISNLDYGVSNEDIKELFSEVGDMK Y IHYD
Sbjct: 61  QHDMFIDQTSGFAVQAGRASAIQTGTKLYISNLDYGVSNEDIKELFSEVGDMKHYSIHYD 120

Query: 172 KSGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKIEIVGTNIATHAVGPPAAVNPFE 231
           KSGRSKGTAEVVFSRR DA AAVKKYNNVQLDGKPMKIEIVGTNIAT A  P AAV PFE
Sbjct: 121 KSGRSKGTAEVVFSRRVDAVAAVKKYNNVQLDGKPMKIEIVGTNIATPAAVPTAAVAPFE 180

Query: 232 NFNGAPRRTVFHVFFYLNQFFRPLLYMYLCKFVSLWCYSFYKQQGRGGLPSRQR----GG 291
           N NG PRR                                  QQGRGGLPSRQR    GG
Sbjct: 181 NLNGFPRR----------------------------------QQGRGGLPSRQRGGGGGG 240

Query: 292 RGSGRGRGRGRGPSEKVSAEDLDADLEKYHAESMQIN 324
           RG GRGRGRGRGPSEKVSAEDLDADLEKYHAESMQIN
Sbjct: 241 RGFGRGRGRGRGPSEKVSAEDLDADLEKYHAESMQIN 243

BLAST of CmoCh02G008260 vs. NCBI nr
Match: KAG7035419.1 (THO complex subunit 4A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 468.8 bits (1205), Expect = 3.9e-128
Identity = 246/280 (87.86%), Postives = 246/280 (87.86%), Query Frame = 0

Query: 44  FLFDFSLNMAAPLDMSLDDIIKNNKKSRSGISRGRGRGSGPGPVRRIPNRAANRTPYSAP 103
           FLFDFSLNMAAPLDMSLDDIIKNNKKSRSGISRGRGRGSGPGPVRRIPNRAANRTPYSAP
Sbjct: 1   FLFDFSLNMAAPLDMSLDDIIKNNKKSRSGISRGRGRGSGPGPVRRIPNRAANRTPYSAP 60

Query: 104 KAPETTWQHDMFVDQGAGFPVQAGRASSIQTGTKLYISNLDYGVSNEDIKELFSEVGDMK 163
           KAPETTWQHDMFVDQGAGFPVQAGRASSIQTGTKLYISNLDYGVSNEDIKELFSEVGDMK
Sbjct: 61  KAPETTWQHDMFVDQGAGFPVQAGRASSIQTGTKLYISNLDYGVSNEDIKELFSEVGDMK 120

Query: 164 RYGIHYDKSGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKIEIVGTNIATHAVGPP 223
           RYGIHYDKSGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKIEIVGTNIATHAVGPP
Sbjct: 121 RYGIHYDKSGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKIEIVGTNIATHAVGPP 180

Query: 224 AAVNPFENFNGAPRRTVFHVFFYLNQFFRPLLYMYLCKFVSLWCYSFYKQQGRGGLPSRQ 283
           AAVNPFENFNGAPRR                                  QQGRGGLPSRQ
Sbjct: 181 AAVNPFENFNGAPRR----------------------------------QQGRGGLPSRQ 240

Query: 284 RGGRGSGRGRGRGRGPSEKVSAEDLDADLEKYHAESMQIN 324
           RGGRGSGRGRGRGRGPSEKVSAEDLDADLEKYHAESMQIN
Sbjct: 241 RGGRGSGRGRGRGRGPSEKVSAEDLDADLEKYHAESMQIN 246

BLAST of CmoCh02G008260 vs. NCBI nr
Match: XP_022947495.1 (THO complex subunit 4A-like [Cucurbita moschata])

HSP 1 Score: 453.4 bits (1165), Expect = 1.7e-123
Identity = 238/272 (87.50%), Postives = 238/272 (87.50%), Query Frame = 0

Query: 52  MAAPLDMSLDDIIKNNKKSRSGISRGRGRGSGPGPVRRIPNRAANRTPYSAPKAPETTWQ 111
           MAAPLDMSLDDIIKNNKKSRSGISRGRGRGSGPGPVRRIPNRAANRTPYSAPKAPETTWQ
Sbjct: 1   MAAPLDMSLDDIIKNNKKSRSGISRGRGRGSGPGPVRRIPNRAANRTPYSAPKAPETTWQ 60

Query: 112 HDMFVDQGAGFPVQAGRASSIQTGTKLYISNLDYGVSNEDIKELFSEVGDMKRYGIHYDK 171
           HDMFVDQGAGFPVQAGRASSIQTGTKLYISNLDYGVSNEDIKELFSEVGDMKRYGIHYDK
Sbjct: 61  HDMFVDQGAGFPVQAGRASSIQTGTKLYISNLDYGVSNEDIKELFSEVGDMKRYGIHYDK 120

Query: 172 SGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKIEIVGTNIATHAVGPPAAVNPFEN 231
           SGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKIEIVGTNIATHAVGPPAAVNPFEN
Sbjct: 121 SGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKIEIVGTNIATHAVGPPAAVNPFEN 180

Query: 232 FNGAPRRTVFHVFFYLNQFFRPLLYMYLCKFVSLWCYSFYKQQGRGGLPSRQRGGRGSGR 291
           FNGAPRR                                  QQGRGGLPSRQRGGRGSGR
Sbjct: 181 FNGAPRR----------------------------------QQGRGGLPSRQRGGRGSGR 238

Query: 292 GRGRGRGPSEKVSAEDLDADLEKYHAESMQIN 324
           GRGRGRGPSEKVSAEDLDADLEKYHAESMQIN
Sbjct: 241 GRGRGRGPSEKVSAEDLDADLEKYHAESMQIN 238

BLAST of CmoCh02G008260 vs. NCBI nr
Match: XP_023006955.1 (THO complex subunit 4A-like [Cucurbita maxima])

HSP 1 Score: 451.8 bits (1161), Expect = 4.9e-123
Identity = 237/272 (87.13%), Postives = 237/272 (87.13%), Query Frame = 0

Query: 52  MAAPLDMSLDDIIKNNKKSRSGISRGRGRGSGPGPVRRIPNRAANRTPYSAPKAPETTWQ 111
           MAAPLDMSLDDIIKNNKKSRSGISRGRGRGSGPGPVRRIPNRAANRTPYSAPKAPETTWQ
Sbjct: 1   MAAPLDMSLDDIIKNNKKSRSGISRGRGRGSGPGPVRRIPNRAANRTPYSAPKAPETTWQ 60

Query: 112 HDMFVDQGAGFPVQAGRASSIQTGTKLYISNLDYGVSNEDIKELFSEVGDMKRYGIHYDK 171
           HDMFVDQGAGFPVQAGRASSIQTGTKLYISNLDYGVSNEDIKELFSEVGDMKRYGIHYDK
Sbjct: 61  HDMFVDQGAGFPVQAGRASSIQTGTKLYISNLDYGVSNEDIKELFSEVGDMKRYGIHYDK 120

Query: 172 SGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKIEIVGTNIATHAVGPPAAVNPFEN 231
           SGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKIEIVGTNIATHAVGPPAAVNPFEN
Sbjct: 121 SGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKIEIVGTNIATHAVGPPAAVNPFEN 180

Query: 232 FNGAPRRTVFHVFFYLNQFFRPLLYMYLCKFVSLWCYSFYKQQGRGGLPSRQRGGRGSGR 291
           FNG PRR                                  QQGRGGLPSRQRGGRGSGR
Sbjct: 181 FNGVPRR----------------------------------QQGRGGLPSRQRGGRGSGR 238

Query: 292 GRGRGRGPSEKVSAEDLDADLEKYHAESMQIN 324
           GRGRGRGPSEKVSAEDLDADLEKYHAESMQIN
Sbjct: 241 GRGRGRGPSEKVSAEDLDADLEKYHAESMQIN 238

BLAST of CmoCh02G008260 vs. NCBI nr
Match: XP_023532287.1 (THO complex subunit 4A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 451.4 bits (1160), Expect = 6.4e-123
Identity = 236/272 (86.76%), Postives = 237/272 (87.13%), Query Frame = 0

Query: 52  MAAPLDMSLDDIIKNNKKSRSGISRGRGRGSGPGPVRRIPNRAANRTPYSAPKAPETTWQ 111
           MAAPLDMSLDDIIKNNKKSRSGISRGRGRGSGPGPVRR+PNRAANRTPYSAPKAPETTWQ
Sbjct: 1   MAAPLDMSLDDIIKNNKKSRSGISRGRGRGSGPGPVRRVPNRAANRTPYSAPKAPETTWQ 60

Query: 112 HDMFVDQGAGFPVQAGRASSIQTGTKLYISNLDYGVSNEDIKELFSEVGDMKRYGIHYDK 171
           HDMFVDQGAGFPVQAGRASSIQTGTKLYISNLDYGVSNEDIKELFSEVGDMKRYGIHYDK
Sbjct: 61  HDMFVDQGAGFPVQAGRASSIQTGTKLYISNLDYGVSNEDIKELFSEVGDMKRYGIHYDK 120

Query: 172 SGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKIEIVGTNIATHAVGPPAAVNPFEN 231
           SGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKIEIVGTNIATHAVGPPAAVNPFEN
Sbjct: 121 SGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKIEIVGTNIATHAVGPPAAVNPFEN 180

Query: 232 FNGAPRRTVFHVFFYLNQFFRPLLYMYLCKFVSLWCYSFYKQQGRGGLPSRQRGGRGSGR 291
           FNG PRR                                  QQGRGGLPSRQRGGRGSGR
Sbjct: 181 FNGVPRR----------------------------------QQGRGGLPSRQRGGRGSGR 238

Query: 292 GRGRGRGPSEKVSAEDLDADLEKYHAESMQIN 324
           GRGRGRGPSEKVSAEDLDADLEKYHAESMQIN
Sbjct: 241 GRGRGRGPSEKVSAEDLDADLEKYHAESMQIN 238

BLAST of CmoCh02G008260 vs. NCBI nr
Match: KAG6605476.1 (THO complex subunit 4A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 434.9 bits (1117), Expect = 6.2e-118
Identity = 229/263 (87.07%), Postives = 229/263 (87.07%), Query Frame = 0

Query: 52  MAAPLDMSLDDIIKNNKKSRSGISRGRGRGSGPGPVRRIPNRAANRTPYSAPKAPETTWQ 111
           MAAPLDMSLDDIIKNNKKSRSGISRGRGRGSGPGPVRRIPNRAANRTPYSAPKAPETTWQ
Sbjct: 1   MAAPLDMSLDDIIKNNKKSRSGISRGRGRGSGPGPVRRIPNRAANRTPYSAPKAPETTWQ 60

Query: 112 HDMFVDQGAGFPVQAGRASSIQTGTKLYISNLDYGVSNEDIKELFSEVGDMKRYGIHYDK 171
           HDMFVDQGAGFPVQAGRASSIQTGTKLYISNLDYGVSNEDIKELFSEVGDMKRYGIHYDK
Sbjct: 61  HDMFVDQGAGFPVQAGRASSIQTGTKLYISNLDYGVSNEDIKELFSEVGDMKRYGIHYDK 120

Query: 172 SGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKIEIVGTNIATHAVGPPAAVNPFEN 231
           SGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKIEIVGTNIATHAVGPPAAVNPFEN
Sbjct: 121 SGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKIEIVGTNIATHAVGPPAAVNPFEN 180

Query: 232 FNGAPRRTVFHVFFYLNQFFRPLLYMYLCKFVSLWCYSFYKQQGRGGLPSRQRGGRGSGR 291
           FNGAPRR                                  QQGRGGLPSRQRGGRGSGR
Sbjct: 181 FNGAPRR----------------------------------QQGRGGLPSRQRGGRGSGR 229

Query: 292 GRGRGRGPSEKVSAEDLDADLEK 315
           GRGRGRGPSEKVSAEDLDADLEK
Sbjct: 241 GRGRGRGPSEKVSAEDLDADLEK 229

BLAST of CmoCh02G008260 vs. TAIR 10
Match: AT5G59950.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 257.7 bits (657), Expect = 1.3e-68
Identity = 158/284 (55.63%), Postives = 189/284 (66.55%), Query Frame = 0

Query: 52  MAAPLDMSLDDIIKNNKKSRSGI--SRGRGRGSGPGPVRR-IPNRAANRT-PYSAPKAPE 111
           M+  LDMSLDD+I  N+KSR G   +RG G GSGPGP RR  PNR + R+ PY + KAPE
Sbjct: 1   MSTGLDMSLDDMIAKNRKSRGGAGPARGTGSGSGPGPTRRNNPNRKSTRSAPYQSAKAPE 60

Query: 112 TTWQHDMFVDQGAGFPVQAGRASS-IQTGTKLYISNLDYGVSNEDIKELFSEVGDMKRYG 171
           +TW HDMF D+      ++GR+S+ I+TGTKLYISNLDYGV NEDIKELF+EVG++KRY 
Sbjct: 61  STWGHDMFSDRSEDH--RSGRSSAGIETGTKLYISNLDYGVMNEDIKELFAEVGELKRYT 120

Query: 172 IHYDKSGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKIEIVGTNIATHAVGPPAAV 231
           +H+D+SGRSKGTAEVV+SRRGDA AAVKKYN+VQLDGKPMKIEIVGTN+ T A   P+  
Sbjct: 121 VHFDRSGRSKGTAEVVYSRRGDALAAVKKYNDVQLDGKPMKIEIVGTNLQTAAA--PSGR 180

Query: 232 NPFENFNGAPRRTVFHVFFYLNQFFRPLLYMYLCKFVSLWCYSFYKQQGRGGLPSRQRGG 291
               N NGAP R                                   QGRGG   +QRGG
Sbjct: 181 PANGNSNGAPWR---------------------------------GGQGRGG---QQRGG 240

Query: 292 -------RGSGRGRGRGRGPSEKVSAEDLDADLEKYHAESMQIN 324
                   G GRGR  G+GP+EK+SAEDLDADL+KYH+  M+ N
Sbjct: 241 GRGGGGRGGGGRGRRPGKGPAEKISAEDLDADLDKYHSGDMETN 244

BLAST of CmoCh02G008260 vs. TAIR 10
Match: AT5G59950.5 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 256.5 bits (654), Expect = 2.8e-68
Identity = 158/284 (55.63%), Postives = 189/284 (66.55%), Query Frame = 0

Query: 52  MAAPLDMSLDDIIKNNKKSRSGI--SRGRGRGSGPGPVRR-IPNRAANRT-PYSAPKAPE 111
           M+  LDMSLDD+I  N+KSR G   +RG G GSGPGP RR  PNR + R+ PY + KAPE
Sbjct: 1   MSTGLDMSLDDMIAKNRKSRGGAGPARGTGSGSGPGPTRRNNPNRKSTRSAPYQSAKAPE 60

Query: 112 TTWQHDMFVDQGAGFPVQAGRASS-IQTGTKLYISNLDYGVSNEDIKELFSEVGDMKRYG 171
           +TW HDMF D+      ++GR+S+ I+TGTKLYISNLDYGV NEDIKELF+EVG++KRY 
Sbjct: 61  STWGHDMFSDRSEDH--RSGRSSAGIETGTKLYISNLDYGVMNEDIKELFAEVGELKRYT 120

Query: 172 IHYDKSGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKIEIVGTNIATHAVGPPAAV 231
           +H+D+SGRSKGTAEVV+SRRGDA AAVKKYN+VQLDGKPMKIEIVGTN+ T A   P+  
Sbjct: 121 VHFDRSGRSKGTAEVVYSRRGDALAAVKKYNDVQLDGKPMKIEIVGTNLQTAAA--PSGR 180

Query: 232 NPFENFNGAPRRTVFHVFFYLNQFFRPLLYMYLCKFVSLWCYSFYKQQGRGGLPSRQRGG 291
               N NGAP                             W       QGRGG   +QRGG
Sbjct: 181 PANGNSNGAP-----------------------------WSRG---GQGRGG---QQRGG 240

Query: 292 -------RGSGRGRGRGRGPSEKVSAEDLDADLEKYHAESMQIN 324
                   G GRGR  G+GP+EK+SAEDLDADL+KYH+  M+ N
Sbjct: 241 GRGGGGRGGGGRGRRPGKGPAEKISAEDLDADLDKYHSGDMETN 245

BLAST of CmoCh02G008260 vs. TAIR 10
Match: AT5G59950.3 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 251.5 bits (641), Expect = 9.0e-67
Identity = 157/284 (55.28%), Postives = 188/284 (66.20%), Query Frame = 0

Query: 52  MAAPLDMSLDDIIKNNKKSRSGI--SRGRGRGSGPGPVRR-IPNRAANRT-PYSAPKAPE 111
           M+  LDMSLDD+I  N+KSR G   +RG G GSGPGP RR  PNR + R+ PY +  APE
Sbjct: 1   MSTGLDMSLDDMIAKNRKSRGGAGPARGTGSGSGPGPTRRNNPNRKSTRSAPYQS--APE 60

Query: 112 TTWQHDMFVDQGAGFPVQAGRASS-IQTGTKLYISNLDYGVSNEDIKELFSEVGDMKRYG 171
           +TW HDMF D+      ++GR+S+ I+TGTKLYISNLDYGV NEDIKELF+EVG++KRY 
Sbjct: 61  STWGHDMFSDRSEDH--RSGRSSAGIETGTKLYISNLDYGVMNEDIKELFAEVGELKRYT 120

Query: 172 IHYDKSGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKIEIVGTNIATHAVGPPAAV 231
           +H+D+SGRSKGTAEVV+SRRGDA AAVKKYN+VQLDGKPMKIEIVGTN+ T A   P+  
Sbjct: 121 VHFDRSGRSKGTAEVVYSRRGDALAAVKKYNDVQLDGKPMKIEIVGTNLQTAAA--PSGR 180

Query: 232 NPFENFNGAPRRTVFHVFFYLNQFFRPLLYMYLCKFVSLWCYSFYKQQGRGGLPSRQRGG 291
               N NGAP R                                   QGRGG   +QRGG
Sbjct: 181 PANGNSNGAPWR---------------------------------GGQGRGG---QQRGG 240

Query: 292 -------RGSGRGRGRGRGPSEKVSAEDLDADLEKYHAESMQIN 324
                   G GRGR  G+GP+EK+SAEDLDADL+KYH+  M+ N
Sbjct: 241 GRGGGGRGGGGRGRRPGKGPAEKISAEDLDADLDKYHSGDMETN 242

BLAST of CmoCh02G008260 vs. TAIR 10
Match: AT5G02530.2 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 230.7 bits (587), Expect = 1.6e-60
Identity = 161/305 (52.79%), Postives = 180/305 (59.02%), Query Frame = 0

Query: 52  MAAPLDMSLDDIIKNNKK-----SRSGISRGR------GRGSGPGPVRRIPNRAANRT-P 111
           M+  LDMSLDDIIK+N+K      R GI  G       G GS  GP RR  NR   RT P
Sbjct: 1   MSGGLDMSLDDIIKSNRKPTGSRGRGGIGGGNNTGGRGGSGSNSGPSRRFANRVGARTAP 60

Query: 112 YSAP----KAPETTWQHDMFVDQ-------GAGFPVQAGRASSIQTGTKLYISNLDYGVS 171
           YS P    +A +  WQ+D+F          G       G  SSI+TGTKLYISNLDYGVS
Sbjct: 61  YSRPIQQQQAHDAMWQNDVFATDASVAAAFGHHQTAVVGGGSSIETGTKLYISNLDYGVS 120

Query: 172 NEDIKELFSEVGDMKRYGIHYDKSGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKI 231
           NEDIKELFSEVGD+KRYGIHYD+SGRSKGTAEVVFSRRGDA AAVK+YNNVQLDGK MKI
Sbjct: 121 NEDIKELFSEVGDLKRYGIHYDRSGRSKGTAEVVFSRRGDALAAVKRYNNVQLDGKLMKI 180

Query: 232 EIVGTNIATHAVGPPAAVN-PF----------ENFNGAPRRTVFHVFFYLNQFF-RPLLY 291
           EIVGTN++  A+   A    PF          ENFNG         F     F  RP   
Sbjct: 181 EIVGTNLSAPALPILATAQIPFPTNGILGNFNENFNGNFNGNFNGNFRGRGGFMGRPR-- 240

Query: 292 MYLCKFVSLWCYSFYKQQGRGGLPSRQRGGRGSGRGRGRGRGPSEKVSAEDLDADLEKYH 322
                        F     RGG  +R RGGRGSG     GRG  E VSAEDLDA+L+KYH
Sbjct: 241 ----------GGGFGGGNFRGGRGARGRGGRGSG-----GRGRDENVSAEDLDAELDKYH 288

BLAST of CmoCh02G008260 vs. TAIR 10
Match: AT5G02530.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 229.6 bits (584), Expect = 3.7e-60
Identity = 161/317 (50.79%), Postives = 183/317 (57.73%), Query Frame = 0

Query: 52  MAAPLDMSLDDIIKNNKK-----SRSGISRGR------GRGSGPGPVRRIPNRAANRT-P 111
           M+  LDMSLDDIIK+N+K      R GI  G       G GS  GP RR  NR   RT P
Sbjct: 1   MSGGLDMSLDDIIKSNRKPTGSRGRGGIGGGNNTGGRGGSGSNSGPSRRFANRVGARTAP 60

Query: 112 YSAP----KAPETTWQHDMFVDQ-------GAGFPVQAGRASSIQTGTKLYISNLDYGVS 171
           YS P    +A +  WQ+D+F          G       G  SSI+TGTKLYISNLDYGVS
Sbjct: 61  YSRPIQQQQAHDAMWQNDVFATDASVAAAFGHHQTAVVGGGSSIETGTKLYISNLDYGVS 120

Query: 172 NEDIKELFSEVGDMKRYGIHYDKSGRSKGTAEVVFSRRGDAAAAVKKYNNVQLDGKPMKI 231
           NEDIKELFSEVGD+KRYGIHYD+SGRSKGTAEVVFSRRGDA AAVK+YNNVQLDGK MKI
Sbjct: 121 NEDIKELFSEVGDLKRYGIHYDRSGRSKGTAEVVFSRRGDALAAVKRYNNVQLDGKLMKI 180

Query: 232 EIVGTNIATHAVGPPAAVN-PF----------ENFNGAPRRTVFHVFFYLNQFFRPLLYM 291
           EIVGTN++  A+   A    PF          ENFNG      F+  F  N         
Sbjct: 181 EIVGTNLSAPALPILATAQIPFPTNGILGNFNENFNGN-----FNGNFNGN--------- 240

Query: 292 YLCKFVSLWCYSFYKQQGRGGLPSRQRGG-------RGSGRGRGR------GRGPSEKVS 322
                        ++ +GRGG   R RGG       RG    RGR      GRG  E VS
Sbjct: 241 -------------FRGRGRGGFMGRPRGGGFGGGNFRGGRGARGRGGRGSGGRGRDENVS 290

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8L7731.8e-6755.63THO complex subunit 4A OS=Arabidopsis thaliana OX=3702 GN=ALY1 PE=1 SV=1[more]
Q8L7195.2e-5950.79THO complex subunit 4B OS=Arabidopsis thaliana OX=3702 GN=ALY2 PE=1 SV=1[more]
Q6NQ724.0e-4342.48THO complex subunit 4D OS=Arabidopsis thaliana OX=3702 GN=ALY4 PE=1 SV=1[more]
Q94EH85.9e-3940.97THO complex subunit 4C OS=Arabidopsis thaliana OX=3702 GN=ALY3 PE=1 SV=1[more]
Q3T0I41.5e-3739.86THO complex subunit 4 OS=Bos taurus OX=9913 GN=ALYREF PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G7218.1e-12487.50THO complex subunit 4A-like OS=Cucurbita moschata OX=3662 GN=LOC111451345 PE=4 S... [more]
A0A6J1L1M62.4e-12387.13THO complex subunit 4A-like OS=Cucurbita maxima OX=3661 GN=LOC111499593 PE=4 SV=... [more]
A0A0A0KBU27.4e-10980.15RRM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G193610 PE=4 SV... [more]
A0A1S3BK704.8e-10879.78THO complex subunit 4A-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490589 P... [more]
A0A6J1D4W81.7e-10577.98THO complex subunit 4A-like OS=Momordica charantia OX=3673 GN=LOC111017043 PE=4 ... [more]
Match NameE-valueIdentityDescription
KAG7035419.13.9e-12887.86THO complex subunit 4A, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022947495.11.7e-12387.50THO complex subunit 4A-like [Cucurbita moschata][more]
XP_023006955.14.9e-12387.13THO complex subunit 4A-like [Cucurbita maxima][more]
XP_023532287.16.4e-12386.76THO complex subunit 4A-like [Cucurbita pepo subsp. pepo][more]
KAG6605476.16.2e-11887.07THO complex subunit 4A, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT5G59950.11.3e-6855.63RNA-binding (RRM/RBD/RNP motifs) family protein [more]
AT5G59950.52.8e-6855.63RNA-binding (RRM/RBD/RNP motifs) family protein [more]
AT5G59950.39.0e-6755.28RNA-binding (RRM/RBD/RNP motifs) family protein [more]
AT5G02530.21.6e-6052.79RNA-binding (RRM/RBD/RNP motifs) family protein [more]
AT5G02530.13.7e-6050.79RNA-binding (RRM/RBD/RNP motifs) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000504RNA recognition motif domainSMARTSM00360rrm1_1coord: 137..209
e-value: 6.0E-19
score: 79.0
IPR000504RNA recognition motif domainPFAMPF00076RRM_1coord: 138..206
e-value: 4.1E-15
score: 55.4
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 136..213
score: 16.210192
IPR025715Chromatin target of PRMT1 protein, C-terminalSMARTSM01218FoP_duplication_2coord: 251..323
e-value: 3.8E-6
score: 36.4
IPR025715Chromatin target of PRMT1 protein, C-terminalPFAMPF13865FoP_duplicationcoord: 274..318
e-value: 3.0E-7
score: 31.0
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3D3.30.70.330coord: 124..305
e-value: 4.0E-22
score: 80.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 275..323
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 69..106
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 294..316
NoneNo IPR availablePANTHERPTHR19965RNA AND EXPORT FACTOR BINDING PROTEINcoord: 280..322
coord: 52..238
NoneNo IPR availablePANTHERPTHR19965:SF74CHROMATIN TARGET OF PRMT1 PROTEIN-RELATEDcoord: 280..322
coord: 52..238
NoneNo IPR availableCDDcd12680RRM_THOC4coord: 136..210
e-value: 1.02306E-45
score: 147.786
IPR035979RNA-binding domain superfamilySUPERFAMILY54928RNA-binding domain, RBDcoord: 75..210

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh02G008260.1CmoCh02G008260.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006406 mRNA export from nucleus
cellular_component GO:0005634 nucleus
molecular_function GO:0003729 mRNA binding
molecular_function GO:0003676 nucleic acid binding