CmoCh02G007130 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh02G007130
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionGDSL esterase/lipase
LocationCmo_Chr02: 4463806 .. 4465117 (+)
RNA-Seq ExpressionCmoCh02G007130
SyntenyCmoCh02G007130
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCACGAGTGAACTATCTCATTGCTGCTCTATCACTCTATATAATTTGGTCATTATGTATTATCAAATTGGGCAGTACTTCTGATCTTAAAATTTCTCGATCATTTCCTGCCATTCTTATTTTTGGTGATTCTACGGTGGATACTGGAAATAACAACTTTATTTCCACAATTTTCAAGGCTAATTACTCTCCATATGGCAAAGATTTTCCTGGTCATATGGCTACTGGAAGGTTTAGTAATGGAAAACTCATTCCTGACATGGTGGCTTCTAGGTTGGGGATAAAGGAGCTTGTTCCTCCATTTTTGGATCCAAAATTGTCTAAGGATGACGTGAAAACAGGAGTTAGTTTTGCATCAGCTGGCACAGGGTTTGATGATCTAACTGCTACGATATCCAAAGTTATTCCTGTGATGAAGCAAATTGATCTTTTCAAGAACTACACTCAAAGGCTTCAAAGGATTGCGGGCGTGGATGAAAGTAAGAAGATTATTGGTAGCGCTTTGGTTATTATTAGTGCAGGAACTAATGATTTGAACATCAATTTCTACGACATTCCCACAAGGAAACTACAGTACAATATTAGCGGTTACCAAGATTTCATACTGAATAGATTACAAAGCTTTATCAAGGTAAATTATACCGTGTGCCCGTATTAATCATAATCAATGTTTGTTAACACAATAATCAAAATGTTAAAATGTAACTTTTCATCTTCATTATTTGATTGAAAATGAACAGGAAATTTACCAGCTCGGATGTCGCAATATAGTGGTTGCTGGCCTCCCTCCGGTTGGTTGTCTTCCCATTCAAGAGACTGTATCGTTTGAAAATCCCCTACATCGAATATGTTTGGAAGATCAAAACTCGGACTCCAAATCCTATAATCAGAAGCTTTCAAAGTTGTTGAGCAATTTACAATCGCAACTACCTGGAAGCACAATGTTGTATGCTGACATTTACACCCCTCTCATCGACATGATCAATAATCCTCACCAATATGGTAAGTTGGTGCCAATTGAAATAGAAAAATTTATCAACTTAAAAGATCTAACGTCATAAATATTTTGAGGAAATCAAGTTTTAAGAATTGTTTTTAACTAAATTTTGTTTGGGGCATTTGGTTGTATAGGTTTTGAGCATACGAACAGAGGTTGTTGTGGAACTGGATTGGCAGAAGCAGGACCTCTATGTAATGCCCTAACCCCAACGTGTGAAGATCCATCAAAGTACATATTTTGGGATAGCATCCATCCAACTGAATCAGTCTACAAGTCCATCACTGAATCGCTTCTCAAACAATTTTTTTGA

mRNA sequence

ATGGCACGAGTGAACTATCTCATTGCTGCTCTATCACTCTATATAATTTGGTCATTATGTATTATCAAATTGGGCAGTACTTCTGATCTTAAAATTTCTCGATCATTTCCTGCCATTCTTATTTTTGGTGATTCTACGGTGGATACTGGAAATAACAACTTTATTTCCACAATTTTCAAGGCTAATTACTCTCCATATGGCAAAGATTTTCCTGGTCATATGGCTACTGGAAGGTTTAGTAATGGAAAACTCATTCCTGACATGGTGGCTTCTAGGTTGGGGATAAAGGAGCTTGTTCCTCCATTTTTGGATCCAAAATTGTCTAAGGATGACGTGAAAACAGGAGTTAGTTTTGCATCAGCTGGCACAGGGTTTGATGATCTAACTGCTACGATATCCAAAGTTATTCCTGTGATGAAGCAAATTGATCTTTTCAAGAACTACACTCAAAGGCTTCAAAGGATTGCGGGCGTGGATGAAAGTAAGAAGATTATTGGTAGCGCTTTGGTTATTATTAGTGCAGGAACTAATGATTTGAACATCAATTTCTACGACATTCCCACAAGGAAACTACAGTACAATATTAGCGGTTACCAAGATTTCATACTGAATAGATTACAAAGCTTTATCAAGGAAATTTACCAGCTCGGATGTCGCAATATAGTGGTTGCTGGCCTCCCTCCGGTTGGTTGTCTTCCCATTCAAGAGACTGTATCGTTTGAAAATCCCCTACATCGAATATGTTTGGAAGATCAAAACTCGGACTCCAAATCCTATAATCAGAAGCTTTCAAAGTTGTTGAGCAATTTACAATCGCAACTACCTGGAAGCACAATGTTGTATGCTGACATTTACACCCCTCTCATCGACATGATCAATAATCCTCACCAATATGGTTTTGAGCATACGAACAGAGGTTGTTGTGGAACTGGATTGGCAGAAGCAGGACCTCTATGTAATGCCCTAACCCCAACGTGTGAAGATCCATCAAAGTACATATTTTGGGATAGCATCCATCCAACTGAATCAGTCTACAAGTCCATCACTGAATCGCTTCTCAAACAATTTTTTTGA

Coding sequence (CDS)

ATGGCACGAGTGAACTATCTCATTGCTGCTCTATCACTCTATATAATTTGGTCATTATGTATTATCAAATTGGGCAGTACTTCTGATCTTAAAATTTCTCGATCATTTCCTGCCATTCTTATTTTTGGTGATTCTACGGTGGATACTGGAAATAACAACTTTATTTCCACAATTTTCAAGGCTAATTACTCTCCATATGGCAAAGATTTTCCTGGTCATATGGCTACTGGAAGGTTTAGTAATGGAAAACTCATTCCTGACATGGTGGCTTCTAGGTTGGGGATAAAGGAGCTTGTTCCTCCATTTTTGGATCCAAAATTGTCTAAGGATGACGTGAAAACAGGAGTTAGTTTTGCATCAGCTGGCACAGGGTTTGATGATCTAACTGCTACGATATCCAAAGTTATTCCTGTGATGAAGCAAATTGATCTTTTCAAGAACTACACTCAAAGGCTTCAAAGGATTGCGGGCGTGGATGAAAGTAAGAAGATTATTGGTAGCGCTTTGGTTATTATTAGTGCAGGAACTAATGATTTGAACATCAATTTCTACGACATTCCCACAAGGAAACTACAGTACAATATTAGCGGTTACCAAGATTTCATACTGAATAGATTACAAAGCTTTATCAAGGAAATTTACCAGCTCGGATGTCGCAATATAGTGGTTGCTGGCCTCCCTCCGGTTGGTTGTCTTCCCATTCAAGAGACTGTATCGTTTGAAAATCCCCTACATCGAATATGTTTGGAAGATCAAAACTCGGACTCCAAATCCTATAATCAGAAGCTTTCAAAGTTGTTGAGCAATTTACAATCGCAACTACCTGGAAGCACAATGTTGTATGCTGACATTTACACCCCTCTCATCGACATGATCAATAATCCTCACCAATATGGTTTTGAGCATACGAACAGAGGTTGTTGTGGAACTGGATTGGCAGAAGCAGGACCTCTATGTAATGCCCTAACCCCAACGTGTGAAGATCCATCAAAGTACATATTTTGGGATAGCATCCATCCAACTGAATCAGTCTACAAGTCCATCACTGAATCGCTTCTCAAACAATTTTTTTGA

Protein sequence

MARVNYLIAALSLYIIWSLCIIKLGSTSDLKISRSFPAILIFGDSTVDTGNNNFISTIFKANYSPYGKDFPGHMATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSKDDVKTGVSFASAGTGFDDLTATISKVIPVMKQIDLFKNYTQRLQRIAGVDESKKIIGSALVIISAGTNDLNINFYDIPTRKLQYNISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPVGCLPIQETVSFENPLHRICLEDQNSDSKSYNQKLSKLLSNLQSQLPGSTMLYADIYTPLIDMINNPHQYGFEHTNRGCCGTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVYKSITESLLKQFF
Homology
BLAST of CmoCh02G007130 vs. ExPASy Swiss-Prot
Match: O22927 (GDSL esterase/lipase At2g30310 OS=Arabidopsis thaliana OX=3702 GN=At2g30310 PE=2 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 2.1e-109
Identity = 192/315 (60.95%), Postives = 236/315 (74.92%), Query Frame = 0

Query: 36  FPAILIFGDSTVDTGNNNFIS-TIFKANYSPYGKDFPGHMATGRFSNGKLIPDMVASRLG 95
           FPAILIFGDSTVDTGNNN+ S TIFKA + PYG D PGH A GR+SNGK+I D++AS+L 
Sbjct: 32  FPAILIFGDSTVDTGNNNYHSQTIFKAKHLPYGVDLPGHEANGRYSNGKVISDVIASKLN 91

Query: 96  IKELVPPFLDPKLSKDDVKTGVSFASAGTGFDDLTATISKVIPVMKQIDLFKNYTQRLQR 155
           IKELVPPFL P +S  D+ TGVSFASAG G+DD ++  SK IPV +Q  +FKNY  RL+ 
Sbjct: 92  IKELVPPFLQPNISHQDIVTGVSFASAGAGYDDRSSLSSKAIPVSQQPSMFKNYIARLKG 151

Query: 156 IAGVDESKKIIGSALVIISAGTNDLNINFYDIPTRKLQY-NISGYQDFILNRLQSFIKEI 215
           I G  ++ +II +ALV+ISAG ND  +NFYDIPTR+L+Y  I GYQ+FIL RL  F++E+
Sbjct: 152 IVGDKKAMEIINNALVVISAGPNDFILNFYDIPTRRLEYPTIHGYQEFILKRLDGFVREL 211

Query: 216 YQLGCRNIVVAGLPPVGCLPIQETVSFENPLHRICLEDQNSDSKSYNQKLSKLLSNLQSQ 275
           Y LGCRNIVV GLPP+GCLPIQ T    N L R C+E +N DS  YNQKL K L  +Q+ 
Sbjct: 212 YSLGCRNIVVGGLPPMGCLPIQMTAKMRNIL-RFCVEQENKDSVLYNQKLVKKLPEIQAS 271

Query: 276 LPGSTMLYADIYTPLIDMINNPHQYGFEHTNRGCCGTGLAEAGPLCNALTPTCEDPSKYI 335
           LPGS  LYA++Y PL+DMI NP +YGF+ T +GCCGTG  E   +CN LT TC + S ++
Sbjct: 272 LPGSNFLYANVYDPLMDMIQNPSKYGFKETKKGCCGTGYLETTFMCNPLTKTCPNHSDHL 331

Query: 336 FWDSIHPTESVYKSI 349
           FWDSIHP+E+ Y  I
Sbjct: 332 FWDSIHPSEAAYNYI 345

BLAST of CmoCh02G007130 vs. ExPASy Swiss-Prot
Match: Q9LMJ3 (GDSL esterase/lipase At1g06990 OS=Arabidopsis thaliana OX=3702 GN=At1g06990 PE=2 SV=2)

HSP 1 Score: 391.0 bits (1003), Expect = 1.5e-107
Identity = 176/322 (54.66%), Postives = 236/322 (73.29%), Query Frame = 0

Query: 36  FPAILIFGDSTVDTGNNNFISTIFKANYSPYGKDFPGHMATGRFSNGKLIPDMVASRLGI 95
           FPAIL+FGDST+DTGNNN+I T  +AN+ PYG +FPGH ATGRFSNGKLIPD +AS +GI
Sbjct: 35  FPAILVFGDSTIDTGNNNYIKTYIRANFPPYGCNFPGHNATGRFSNGKLIPDFIASLMGI 94

Query: 96  KELVPPFLDPKLSKDDVKTGVSFASAGTGFDDLTATISKVIPVMKQIDLFKNYTQRLQRI 155
           K+ VPPFLDP LS  D+ TGV FASAG+G+D+LT   +  + V KQ D+ ++Y +RL +I
Sbjct: 95  KDTVPPFLDPHLSDSDIITGVCFASAGSGYDNLTDRATSTLSVDKQADMLRSYVERLSQI 154

Query: 156 AGVDESKKIIGSALVIISAGTNDLNINFYDIPTRKLQYNISGYQDFILNRLQSFIKEIYQ 215
            G +++  I+  ALVI+S+GTND N+N YD P+R+ +  + GYQ FIL+ + +F++E+Y 
Sbjct: 155 VGDEKAASIVSEALVIVSSGTNDFNLNLYDTPSRRQKLGVDGYQSFILSNVHNFVQELYD 214

Query: 216 LGCRNIVVAGLPPVGCLPIQETVSFENPLHRICLEDQNSDSKSYNQKLSKLLSNLQSQLP 275
           +GCR I+V GLPPVGCLPIQ T++ +    R C++ QNSDS+ +NQKL   L+ +QS L 
Sbjct: 215 IGCRKIMVLGLPPVGCLPIQMTMAMQKQNERRCIDKQNSDSQEFNQKLKNSLTEMQSNLT 274

Query: 276 GSTMLYADIYTPLIDMINNPHQYGFEHTNRGCCGTGLAEAGPLCNALTPTCEDPSKYIFW 335
           GS + Y DIY  L DM  NP +YG + T RGCCGTG  E   LCNALT  C +P++Y+FW
Sbjct: 275 GSVIFYGDIYGALFDMATNPQRYGLKETTRGCCGTGEIELAYLCNALTRICPNPNQYLFW 334

Query: 336 DSIHPTESVYKSITESLLKQFF 358
           D IHP++  Y  I+ SL++Q F
Sbjct: 335 DDIHPSQIAYIVISLSLVEQIF 356

BLAST of CmoCh02G007130 vs. ExPASy Swiss-Prot
Match: O22918 (GDSL esterase/lipase At2g30220 OS=Arabidopsis thaliana OX=3702 GN=At2g30220 PE=3 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 8.1e-106
Identity = 184/313 (58.79%), Postives = 231/313 (73.80%), Query Frame = 0

Query: 36  FPAILIFGDSTVDTGNNNFIS-TIFKANYSPYGKDFPGHMATGRFSNGKLIPDMVASRLG 95
           FPAILIFGDST DTGNNN+ S  +FKAN+ PYG D PGH A GRFSNGKLI D+++++L 
Sbjct: 31  FPAILIFGDSTADTGNNNYYSQAVFKANHLPYGVDLPGHEANGRFSNGKLISDVISTKLN 90

Query: 96  IKELVPPFLDPKLSKDDVKTGVSFASAGTGFDDLTATISKVIPVMKQIDLFKNYTQRLQR 155
           IKE VPPFL P +S  D+ TGV FASAG G+DD T+  SK IPV +Q  +FKNY  RL+ 
Sbjct: 91  IKEFVPPFLQPNISDQDIVTGVCFASAGAGYDDETSLSSKAIPVSQQPSMFKNYIARLKG 150

Query: 156 IAGVDESKKIIGSALVIISAGTNDLNINFYDIPTRKLQY-NISGYQDFILNRLQSFIKEI 215
           I G  ++ +II +ALV+ISAG ND  +NFYDIP R+L+Y  I GYQDF+L RL  F++E+
Sbjct: 151 IVGDKKAMEIINNALVVISAGPNDFILNFYDIPIRRLEYPTIYGYQDFVLKRLDGFVREL 210

Query: 216 YQLGCRNIVVAGLPPVGCLPIQETVSFENPLHRICLEDQNSDSKSYNQKLSKLLSNLQSQ 275
           Y LGCRNI+V GLPP+GCLPIQ T      L  IC+E +N DS  YNQKL K L  +Q+ 
Sbjct: 211 YSLGCRNILVGGLPPMGCLPIQLTAKLRTIL-GICVEQENKDSILYNQKLVKKLPEIQAS 270

Query: 276 LPGSTMLYADIYTPLIDMINNPHQYGFEHTNRGCCGTGLAEAGPLCNALTPTCEDPSKYI 335
           LPGS  LYA++Y P++DMI NP +YGF+ T +GCCGTG  E   LC +L+ TC + S ++
Sbjct: 271 LPGSKFLYANVYDPVMDMIRNPSKYGFKETKKGCCGTGYLETSFLCTSLSKTCPNHSDHL 330

Query: 336 FWDSIHPTESVYK 347
           FWDSIHP+E+ YK
Sbjct: 331 FWDSIHPSEAAYK 342

BLAST of CmoCh02G007130 vs. ExPASy Swiss-Prot
Match: Q9SIQ2 (GDSL esterase/lipase At2g31550 OS=Arabidopsis thaliana OX=3702 GN=At2g31550 PE=3 SV=3)

HSP 1 Score: 376.7 bits (966), Expect = 2.9e-103
Identity = 181/344 (52.62%), Postives = 247/344 (71.80%), Query Frame = 0

Query: 11  LSLYIIWSLCIIKLGSTSDLKISRSFPAILIFGDSTVDTGNNNF-ISTIFKANYSPYGKD 70
           L+L+I  +L +    + ++      FPAILIFGDSTVDTGNNN+ + TIF+A + PYG D
Sbjct: 9   LTLFITTTL-LASCDAAANATTKPLFPAILIFGDSTVDTGNNNYPLPTIFRAEHFPYGMD 68

Query: 71  FPGHMATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSKDDVKTGVSFASAGTGFDDLT 130
            P   A GRFSNGKLI D++A++L IKE +PPFL P LS  D+ TGV FASAG G+DDLT
Sbjct: 69  LPDGKANGRFSNGKLISDIIATKLNIKEFIPPFLQPNLSDQDILTGVCFASAGAGYDDLT 128

Query: 131 ATISKVIPVMKQIDLFKNYTQRLQRIAGVDESKKIIGSALVIISAGTNDLNINFYDIPTR 190
           +  ++ I V +Q ++FK+Y  RL+ I G  ++ +II +A V++SAG ND  +N+YDIP+R
Sbjct: 129 SLSTQAIRVSEQPNMFKSYIARLKGIVGDKKAMEIINNAFVVVSAGPNDFILNYYDIPSR 188

Query: 191 KLQYN-ISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPVGCLPIQETVSFENPLHRIC 250
           +L+Y  ISGYQDFIL RL++F++E+Y LG RN++V GLPP+GCLPI  T  F N + R C
Sbjct: 189 RLEYPFISGYQDFILKRLENFVRELYSLGVRNVLVGGLPPMGCLPIHMTAKFRN-IFRFC 248

Query: 251 LEDQNSDSKSYNQKLSKLLSNLQSQLPGSTMLYADIYTPLIDMINNPHQYGFEHTNRGCC 310
           LE  N DS  YN+KL KLL  +++ LPGS  LYAD+Y P+++MI NP +YGF+ T RGCC
Sbjct: 249 LEHHNKDSVLYNEKLQKLLPQIEASLPGSKFLYADVYNPMMEMIQNPSKYGFKETKRGCC 308

Query: 311 GTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVYKSITESL 353
           GTG  E   +CN  +P C++ S+++F+DSIHP+E+ Y  I   L
Sbjct: 309 GTGFLETSFMCNVFSPVCQNRSEFMFFDSIHPSEATYNVIGNRL 350

BLAST of CmoCh02G007130 vs. ExPASy Swiss-Prot
Match: Q9SJA9 (GDSL esterase/lipase At2g24560 OS=Arabidopsis thaliana OX=3702 GN=At2g24560 PE=2 SV=2)

HSP 1 Score: 375.2 bits (962), Expect = 8.4e-103
Identity = 182/337 (54.01%), Postives = 247/337 (73.29%), Query Frame = 0

Query: 15  IIWSLCIIKLGSTSDLKISRS----FPAILIFGDSTVDTGNNNFIS-TIFKANYSPYGKD 74
           I ++L I  L S+ D   + +    FPAILIFGDSTVDTGNNN+ S TIFKA + PYG D
Sbjct: 7   ITFTLFIAALLSSCDAATNATSQPLFPAILIFGDSTVDTGNNNYHSQTIFKAKHLPYGID 66

Query: 75  FPGHMATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSKDDVKTGVSFASAGTGFDDLT 134
            P H A+GRF+NGK+  D++A++L IK+ VPPFL P LS  ++ TGV FASAG G+DD T
Sbjct: 67  LPNHKASGRFTNGKIFSDIIATKLNIKQFVPPFLQPNLSDQEIVTGVCFASAGAGYDDHT 126

Query: 135 ATISKVIPVMKQIDLFKNYTQRLQRIAGVDESKKIIGSALVIISAGTNDLNINFYDIPTR 194
           +  ++ I V+ Q  +FKNY  RL+ I G  ++ +II +ALV+ISAG ND  +N+YDIP+R
Sbjct: 127 SLSTQAIRVLDQQKMFKNYIARLKSIVGDKKAMEIIKNALVVISAGPNDFILNYYDIPSR 186

Query: 195 KLQY-NISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPVGCLPIQETVSFENPLHRIC 254
           +L++ +ISGYQDF+L RL +F++E+Y LGCR I+V GLPP+GCLPIQ T  F N L R C
Sbjct: 187 RLEFPHISGYQDFVLQRLDNFVRELYSLGCRKIMVGGLPPMGCLPIQMTAKFRNAL-RFC 246

Query: 255 LEDQNSDSKSYNQKLSKLLSNLQSQLPGSTMLYADIYTPLIDMINNPHQYGFEHTNRGCC 314
           LE +N DS  YNQKL  LL  +++ L GS +LY+++Y P++DM+ NP +YGF+ T RGCC
Sbjct: 247 LEQENRDSVLYNQKLQNLLPQIEASLTGSKILYSNVYDPMMDMMQNPSKYGFKETKRGCC 306

Query: 315 GTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVY 346
           GTG  E   +CNA +PTC + S+++F+DSIHP+E+ Y
Sbjct: 307 GTGHLETSFMCNAFSPTCRNHSEFLFFDSIHPSEATY 342

BLAST of CmoCh02G007130 vs. ExPASy TrEMBL
Match: A0A6J1G7Z0 (GDSL esterase/lipase At2g30310-like OS=Cucurbita moschata OX=3662 GN=LOC111451580 PE=3 SV=1)

HSP 1 Score: 720.7 bits (1859), Expect = 3.0e-204
Identity = 357/357 (100.00%), Postives = 357/357 (100.00%), Query Frame = 0

Query: 1   MARVNYLIAALSLYIIWSLCIIKLGSTSDLKISRSFPAILIFGDSTVDTGNNNFISTIFK 60
           MARVNYLIAALSLYIIWSLCIIKLGSTSDLKISRSFPAILIFGDSTVDTGNNNFISTIFK
Sbjct: 1   MARVNYLIAALSLYIIWSLCIIKLGSTSDLKISRSFPAILIFGDSTVDTGNNNFISTIFK 60

Query: 61  ANYSPYGKDFPGHMATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSKDDVKTGVSFAS 120
           ANYSPYGKDFPGHMATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSKDDVKTGVSFAS
Sbjct: 61  ANYSPYGKDFPGHMATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSKDDVKTGVSFAS 120

Query: 121 AGTGFDDLTATISKVIPVMKQIDLFKNYTQRLQRIAGVDESKKIIGSALVIISAGTNDLN 180
           AGTGFDDLTATISKVIPVMKQIDLFKNYTQRLQRIAGVDESKKIIGSALVIISAGTNDLN
Sbjct: 121 AGTGFDDLTATISKVIPVMKQIDLFKNYTQRLQRIAGVDESKKIIGSALVIISAGTNDLN 180

Query: 181 INFYDIPTRKLQYNISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPVGCLPIQETVSF 240
           INFYDIPTRKLQYNISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPVGCLPIQETVSF
Sbjct: 181 INFYDIPTRKLQYNISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPVGCLPIQETVSF 240

Query: 241 ENPLHRICLEDQNSDSKSYNQKLSKLLSNLQSQLPGSTMLYADIYTPLIDMINNPHQYGF 300
           ENPLHRICLEDQNSDSKSYNQKLSKLLSNLQSQLPGSTMLYADIYTPLIDMINNPHQYGF
Sbjct: 241 ENPLHRICLEDQNSDSKSYNQKLSKLLSNLQSQLPGSTMLYADIYTPLIDMINNPHQYGF 300

Query: 301 EHTNRGCCGTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVYKSITESLLKQFF 358
           EHTNRGCCGTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVYKSITESLLKQFF
Sbjct: 301 EHTNRGCCGTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVYKSITESLLKQFF 357

BLAST of CmoCh02G007130 vs. ExPASy TrEMBL
Match: A0A6J1KWV5 (GDSL esterase/lipase At2g30310-like OS=Cucurbita maxima OX=3661 GN=LOC111499482 PE=3 SV=1)

HSP 1 Score: 673.7 bits (1737), Expect = 4.2e-190
Identity = 329/357 (92.16%), Postives = 341/357 (95.52%), Query Frame = 0

Query: 1   MARVNYLIAALSLYIIWSLCIIKLGSTSDLKISRSFPAILIFGDSTVDTGNNNFISTIFK 60
           MAR NY IAALSL+IIWSLC+ KL   SDLKISR+FPAILIFGDSTVDTGNNNFI TIFK
Sbjct: 1   MARANYFIAALSLHIIWSLCVTKLCRASDLKISRTFPAILIFGDSTVDTGNNNFIPTIFK 60

Query: 61  ANYSPYGKDFPGHMATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSKDDVKTGVSFAS 120
           ANY+PYGKDFPGH+ATGRFSNGKLIPDMVASRLGIKE VPPFLDPKL  DDVKTGVSFAS
Sbjct: 61  ANYAPYGKDFPGHIATGRFSNGKLIPDMVASRLGIKEFVPPFLDPKLLNDDVKTGVSFAS 120

Query: 121 AGTGFDDLTATISKVIPVMKQIDLFKNYTQRLQRIAGVDESKKIIGSALVIISAGTNDLN 180
           AGTGFDDLTATISKVIPVMKQIDLFKNY QRLQRI GVDESKKIIGSALVIISAGTNDLN
Sbjct: 121 AGTGFDDLTATISKVIPVMKQIDLFKNYIQRLQRIVGVDESKKIIGSALVIISAGTNDLN 180

Query: 181 INFYDIPTRKLQYNISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPVGCLPIQETVSF 240
           INFYD+PTRKLQYNISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPP+GCLPIQET+SF
Sbjct: 181 INFYDLPTRKLQYNISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPIGCLPIQETISF 240

Query: 241 ENPLHRICLEDQNSDSKSYNQKLSKLLSNLQSQLPGSTMLYADIYTPLIDMINNPHQYGF 300
           +NPL+RICLEDQNSDSKSYNQKLSKLLSNLQ QLP S MLYADIYTPL+DMINNPHQYGF
Sbjct: 241 QNPLNRICLEDQNSDSKSYNQKLSKLLSNLQPQLPRSKMLYADIYTPLMDMINNPHQYGF 300

Query: 301 EHTNRGCCGTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVYKSITESLLKQFF 358
           EHTN GCCGTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVYKSITESL+KQFF
Sbjct: 301 EHTNTGCCGTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVYKSITESLIKQFF 357

BLAST of CmoCh02G007130 vs. ExPASy TrEMBL
Match: A0A6J1L373 (GDSL esterase/lipase At2g30310-like OS=Cucurbita maxima OX=3661 GN=LOC111499480 PE=3 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 7.5e-171
Identity = 298/356 (83.71%), Postives = 319/356 (89.61%), Query Frame = 0

Query: 1   MARVNYLIAALSLYIIWSLCIIKLGSTSDLKISRSFPAILIFGDSTVDTGNNNFISTIFK 60
           MARVNYLIAALSL I+WSL +IKL S SDLK  RSFPAILIFGDSTVDTGNNNFI TI K
Sbjct: 1   MARVNYLIAALSLQIVWSLYMIKLCSASDLKNFRSFPAILIFGDSTVDTGNNNFIPTIIK 60

Query: 61  ANYSPYGKDFPGHMATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSKDDVKTGVSFAS 120
           ANYSPYGKDFP H+ATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLS DDVKTGVSFAS
Sbjct: 61  ANYSPYGKDFPNHIATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSNDDVKTGVSFAS 120

Query: 121 AGTGFDDLTATISKVIPVMKQIDLFKNYTQRLQRIAGVDESKKIIGSALVIISAGTNDLN 180
           AG+GFDDLT  I KVIP+M Q+D+FKNY QRL  I GVDESKKIIGSALVI+SAGTND  
Sbjct: 121 AGSGFDDLTTMIYKVIPMMMQVDMFKNYIQRLTEIVGVDESKKIIGSALVILSAGTNDFT 180

Query: 181 INFYDIPTRKLQYNISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPVGCLPIQETVSF 240
            NFYD+P RKLQYNISGYQDFI +RLQSFIKEIY+LGCRNIVVAGLPP+GCLPIQET+SF
Sbjct: 181 KNFYDLPVRKLQYNISGYQDFIQDRLQSFIKEIYELGCRNIVVAGLPPIGCLPIQETISF 240

Query: 241 ENPLHRICLEDQNSDSKSYNQKLSKLLSNLQSQLPGSTMLYADIYTPLIDMINNPHQYGF 300
           + PL RICLEDQNSDS SYNQKLSKLL NL SQL GST+LYADIYTPLIDM+NNPH+YGF
Sbjct: 241 QLPLTRICLEDQNSDSISYNQKLSKLLGNLPSQLHGSTILYADIYTPLIDMVNNPHKYGF 300

Query: 301 EHTNRGCCGTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVYKSITESLLKQF 357
           EHTN GCCGTGLAE  PLCNALTPTCED SKY+FWDS+HPTE+ YK +TE  LKQF
Sbjct: 301 EHTNTGCCGTGLAEVAPLCNALTPTCEDSSKYLFWDSVHPTEAAYKFMTELFLKQF 356

BLAST of CmoCh02G007130 vs. ExPASy TrEMBL
Match: A0A6J1G7I3 (GDSL esterase/lipase At2g30310-like OS=Cucurbita moschata OX=3662 GN=LOC111451569 PE=3 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 1.7e-170
Identity = 297/356 (83.43%), Postives = 318/356 (89.33%), Query Frame = 0

Query: 1   MARVNYLIAALSLYIIWSLCIIKLGSTSDLKISRSFPAILIFGDSTVDTGNNNFISTIFK 60
           MARVNYLIAALSL I+WSLC+ KL S SDLK   SFPAILIFGDSTVDTGNNNFI TI K
Sbjct: 1   MARVNYLIAALSLKIVWSLCMSKLCSASDLKNFGSFPAILIFGDSTVDTGNNNFIPTITK 60

Query: 61  ANYSPYGKDFPGHMATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSKDDVKTGVSFAS 120
           ANYSPYGKDF GH+ATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLS DDVKTGV+FAS
Sbjct: 61  ANYSPYGKDFSGHIATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSNDDVKTGVNFAS 120

Query: 121 AGTGFDDLTATISKVIPVMKQIDLFKNYTQRLQRIAGVDESKKIIGSALVIISAGTNDLN 180
           AG+GFDDLT  ISKVIP+MKQ+D+FKNY QRL    GVDESKKIIGSALVI+SAGTND  
Sbjct: 121 AGSGFDDLTTMISKVIPMMKQVDMFKNYIQRLTESVGVDESKKIIGSALVILSAGTNDFT 180

Query: 181 INFYDIPTRKLQYNISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPVGCLPIQETVSF 240
            NFYD+P RKLQYNISGYQDFI  RLQ+FIKEIY LGCRNIVVAGLPP+GCLPIQET+SF
Sbjct: 181 KNFYDLPVRKLQYNISGYQDFIQERLQNFIKEIYDLGCRNIVVAGLPPIGCLPIQETISF 240

Query: 241 ENPLHRICLEDQNSDSKSYNQKLSKLLSNLQSQLPGSTMLYADIYTPLIDMINNPHQYGF 300
           + PL RICLEDQNSDS SYNQKLSKLL NLQSQL GST+LYADIYTPLIDM+NNPH+YGF
Sbjct: 241 QPPLKRICLEDQNSDSISYNQKLSKLLRNLQSQLHGSTILYADIYTPLIDMVNNPHKYGF 300

Query: 301 EHTNRGCCGTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVYKSITESLLKQF 357
           EHTN GCCGTGLAE  PLCNALTPTCED SKY+FWDS+HPTE+ YK +TE  LKQF
Sbjct: 301 EHTNTGCCGTGLAEVAPLCNALTPTCEDSSKYLFWDSVHPTEAAYKFMTELFLKQF 356

BLAST of CmoCh02G007130 vs. ExPASy TrEMBL
Match: A0A6J1KWV1 (GDSL esterase/lipase At2g30310-like OS=Cucurbita maxima OX=3661 GN=LOC111499475 PE=3 SV=1)

HSP 1 Score: 606.7 bits (1563), Expect = 6.3e-170
Identity = 297/356 (83.43%), Postives = 318/356 (89.33%), Query Frame = 0

Query: 1   MARVNYLIAALSLYIIWSLCIIKLGSTSDLKISRSFPAILIFGDSTVDTGNNNFISTIFK 60
           MARVNYLIAALSL I+WSL +IKL S SDLK  RSFPAILIFGDSTVDTGNNNFI TI K
Sbjct: 1   MARVNYLIAALSLQIVWSLYMIKLCSASDLKNFRSFPAILIFGDSTVDTGNNNFIPTIIK 60

Query: 61  ANYSPYGKDFPGHMATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSKDDVKTGVSFAS 120
           ANYSPYGKDFP H+ATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLS DDVKTGVSFAS
Sbjct: 61  ANYSPYGKDFPNHIATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSNDDVKTGVSFAS 120

Query: 121 AGTGFDDLTATISKVIPVMKQIDLFKNYTQRLQRIAGVDESKKIIGSALVIISAGTNDLN 180
           AG+GFDDLT  I KVIP+M Q+D+FKNY QRL  I GVDESKKIIGSALVI+SAGTND  
Sbjct: 121 AGSGFDDLTTMIYKVIPMMMQVDMFKNYIQRLTEIVGVDESKKIIGSALVILSAGTNDFT 180

Query: 181 INFYDIPTRKLQYNISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPVGCLPIQETVSF 240
            NFYD+P RKLQYNISGYQDFI +RLQSFIKEIY+LGCRNIVVAGLPP+GCLPIQET+S 
Sbjct: 181 KNFYDLPVRKLQYNISGYQDFIQDRLQSFIKEIYELGCRNIVVAGLPPIGCLPIQETISS 240

Query: 241 ENPLHRICLEDQNSDSKSYNQKLSKLLSNLQSQLPGSTMLYADIYTPLIDMINNPHQYGF 300
           + PL RICLEDQNSDS SYNQKLSKLL NL SQL GST+LYADIYTPLIDM+NNPH+YGF
Sbjct: 241 QLPLTRICLEDQNSDSISYNQKLSKLLGNLPSQLHGSTILYADIYTPLIDMVNNPHKYGF 300

Query: 301 EHTNRGCCGTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVYKSITESLLKQF 357
           EHTN GCCGTGLAE  PLCNALTPTCED SKY+FWDS+HPTE+ YK +TE  LKQF
Sbjct: 301 EHTNTGCCGTGLAEVAPLCNALTPTCEDSSKYLFWDSVHPTEAAYKFMTELFLKQF 356

BLAST of CmoCh02G007130 vs. NCBI nr
Match: XP_022947820.1 (GDSL esterase/lipase At2g30310-like [Cucurbita moschata])

HSP 1 Score: 720.7 bits (1859), Expect = 6.2e-204
Identity = 357/357 (100.00%), Postives = 357/357 (100.00%), Query Frame = 0

Query: 1   MARVNYLIAALSLYIIWSLCIIKLGSTSDLKISRSFPAILIFGDSTVDTGNNNFISTIFK 60
           MARVNYLIAALSLYIIWSLCIIKLGSTSDLKISRSFPAILIFGDSTVDTGNNNFISTIFK
Sbjct: 1   MARVNYLIAALSLYIIWSLCIIKLGSTSDLKISRSFPAILIFGDSTVDTGNNNFISTIFK 60

Query: 61  ANYSPYGKDFPGHMATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSKDDVKTGVSFAS 120
           ANYSPYGKDFPGHMATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSKDDVKTGVSFAS
Sbjct: 61  ANYSPYGKDFPGHMATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSKDDVKTGVSFAS 120

Query: 121 AGTGFDDLTATISKVIPVMKQIDLFKNYTQRLQRIAGVDESKKIIGSALVIISAGTNDLN 180
           AGTGFDDLTATISKVIPVMKQIDLFKNYTQRLQRIAGVDESKKIIGSALVIISAGTNDLN
Sbjct: 121 AGTGFDDLTATISKVIPVMKQIDLFKNYTQRLQRIAGVDESKKIIGSALVIISAGTNDLN 180

Query: 181 INFYDIPTRKLQYNISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPVGCLPIQETVSF 240
           INFYDIPTRKLQYNISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPVGCLPIQETVSF
Sbjct: 181 INFYDIPTRKLQYNISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPVGCLPIQETVSF 240

Query: 241 ENPLHRICLEDQNSDSKSYNQKLSKLLSNLQSQLPGSTMLYADIYTPLIDMINNPHQYGF 300
           ENPLHRICLEDQNSDSKSYNQKLSKLLSNLQSQLPGSTMLYADIYTPLIDMINNPHQYGF
Sbjct: 241 ENPLHRICLEDQNSDSKSYNQKLSKLLSNLQSQLPGSTMLYADIYTPLIDMINNPHQYGF 300

Query: 301 EHTNRGCCGTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVYKSITESLLKQFF 358
           EHTNRGCCGTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVYKSITESLLKQFF
Sbjct: 301 EHTNRGCCGTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVYKSITESLLKQFF 357

BLAST of CmoCh02G007130 vs. NCBI nr
Match: XP_023533087.1 (GDSL esterase/lipase At2g30310-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 692.2 bits (1785), Expect = 2.4e-195
Identity = 345/357 (96.64%), Postives = 347/357 (97.20%), Query Frame = 0

Query: 1   MARVNYLIAALSLYIIWSLCIIKLGSTSDLKISRSFPAILIFGDSTVDTGNNNFISTIFK 60
           MARVNYLI ALSL+II SLCI KL S SDLKISRSFPAILIFGDSTVDTGNNNFISTIFK
Sbjct: 1   MARVNYLIGALSLHIILSLCISKLCSASDLKISRSFPAILIFGDSTVDTGNNNFISTIFK 60

Query: 61  ANYSPYGKDFPGHMATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSKDDVKTGVSFAS 120
           ANYSPYGKDFPGHMATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLS DDVKTGVSFAS
Sbjct: 61  ANYSPYGKDFPGHMATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSNDDVKTGVSFAS 120

Query: 121 AGTGFDDLTATISKVIPVMKQIDLFKNYTQRLQRIAGVDESKKIIGSALVIISAGTNDLN 180
           AGTGFDDLTATISKVIPVMKQIDLFKNYT+RLQRI GVDESKKIIGSALVIISAGTNDLN
Sbjct: 121 AGTGFDDLTATISKVIPVMKQIDLFKNYTKRLQRIVGVDESKKIIGSALVIISAGTNDLN 180

Query: 181 INFYDIPTRKLQYNISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPVGCLPIQETVSF 240
           INFYDIPTRKLQYNISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPVGCLPIQETVSF
Sbjct: 181 INFYDIPTRKLQYNISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPVGCLPIQETVSF 240

Query: 241 ENPLHRICLEDQNSDSKSYNQKLSKLLSNLQSQLPGSTMLYADIYTPLIDMINNPHQYGF 300
           ENPLHRICLEDQNSDSKSYNQKLSKLLSNLQ QLPGSTMLYADIYTPLIDMINNP QYGF
Sbjct: 241 ENPLHRICLEDQNSDSKSYNQKLSKLLSNLQPQLPGSTMLYADIYTPLIDMINNPRQYGF 300

Query: 301 EHTNRGCCGTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVYKSITESLLKQFF 358
           EHTN GCCGTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVYKSITESLLKQFF
Sbjct: 301 EHTNTGCCGTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVYKSITESLLKQFF 357

BLAST of CmoCh02G007130 vs. NCBI nr
Match: XP_023006812.1 (GDSL esterase/lipase At2g30310-like [Cucurbita maxima])

HSP 1 Score: 673.7 bits (1737), Expect = 8.7e-190
Identity = 329/357 (92.16%), Postives = 341/357 (95.52%), Query Frame = 0

Query: 1   MARVNYLIAALSLYIIWSLCIIKLGSTSDLKISRSFPAILIFGDSTVDTGNNNFISTIFK 60
           MAR NY IAALSL+IIWSLC+ KL   SDLKISR+FPAILIFGDSTVDTGNNNFI TIFK
Sbjct: 1   MARANYFIAALSLHIIWSLCVTKLCRASDLKISRTFPAILIFGDSTVDTGNNNFIPTIFK 60

Query: 61  ANYSPYGKDFPGHMATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSKDDVKTGVSFAS 120
           ANY+PYGKDFPGH+ATGRFSNGKLIPDMVASRLGIKE VPPFLDPKL  DDVKTGVSFAS
Sbjct: 61  ANYAPYGKDFPGHIATGRFSNGKLIPDMVASRLGIKEFVPPFLDPKLLNDDVKTGVSFAS 120

Query: 121 AGTGFDDLTATISKVIPVMKQIDLFKNYTQRLQRIAGVDESKKIIGSALVIISAGTNDLN 180
           AGTGFDDLTATISKVIPVMKQIDLFKNY QRLQRI GVDESKKIIGSALVIISAGTNDLN
Sbjct: 121 AGTGFDDLTATISKVIPVMKQIDLFKNYIQRLQRIVGVDESKKIIGSALVIISAGTNDLN 180

Query: 181 INFYDIPTRKLQYNISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPVGCLPIQETVSF 240
           INFYD+PTRKLQYNISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPP+GCLPIQET+SF
Sbjct: 181 INFYDLPTRKLQYNISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPIGCLPIQETISF 240

Query: 241 ENPLHRICLEDQNSDSKSYNQKLSKLLSNLQSQLPGSTMLYADIYTPLIDMINNPHQYGF 300
           +NPL+RICLEDQNSDSKSYNQKLSKLLSNLQ QLP S MLYADIYTPL+DMINNPHQYGF
Sbjct: 241 QNPLNRICLEDQNSDSKSYNQKLSKLLSNLQPQLPRSKMLYADIYTPLMDMINNPHQYGF 300

Query: 301 EHTNRGCCGTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVYKSITESLLKQFF 358
           EHTN GCCGTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVYKSITESL+KQFF
Sbjct: 301 EHTNTGCCGTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVYKSITESLIKQFF 357

BLAST of CmoCh02G007130 vs. NCBI nr
Match: XP_023006809.1 (GDSL esterase/lipase At2g30310-like [Cucurbita maxima])

HSP 1 Score: 609.8 bits (1571), Expect = 1.5e-170
Identity = 298/356 (83.71%), Postives = 319/356 (89.61%), Query Frame = 0

Query: 1   MARVNYLIAALSLYIIWSLCIIKLGSTSDLKISRSFPAILIFGDSTVDTGNNNFISTIFK 60
           MARVNYLIAALSL I+WSL +IKL S SDLK  RSFPAILIFGDSTVDTGNNNFI TI K
Sbjct: 1   MARVNYLIAALSLQIVWSLYMIKLCSASDLKNFRSFPAILIFGDSTVDTGNNNFIPTIIK 60

Query: 61  ANYSPYGKDFPGHMATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSKDDVKTGVSFAS 120
           ANYSPYGKDFP H+ATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLS DDVKTGVSFAS
Sbjct: 61  ANYSPYGKDFPNHIATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSNDDVKTGVSFAS 120

Query: 121 AGTGFDDLTATISKVIPVMKQIDLFKNYTQRLQRIAGVDESKKIIGSALVIISAGTNDLN 180
           AG+GFDDLT  I KVIP+M Q+D+FKNY QRL  I GVDESKKIIGSALVI+SAGTND  
Sbjct: 121 AGSGFDDLTTMIYKVIPMMMQVDMFKNYIQRLTEIVGVDESKKIIGSALVILSAGTNDFT 180

Query: 181 INFYDIPTRKLQYNISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPVGCLPIQETVSF 240
            NFYD+P RKLQYNISGYQDFI +RLQSFIKEIY+LGCRNIVVAGLPP+GCLPIQET+SF
Sbjct: 181 KNFYDLPVRKLQYNISGYQDFIQDRLQSFIKEIYELGCRNIVVAGLPPIGCLPIQETISF 240

Query: 241 ENPLHRICLEDQNSDSKSYNQKLSKLLSNLQSQLPGSTMLYADIYTPLIDMINNPHQYGF 300
           + PL RICLEDQNSDS SYNQKLSKLL NL SQL GST+LYADIYTPLIDM+NNPH+YGF
Sbjct: 241 QLPLTRICLEDQNSDSISYNQKLSKLLGNLPSQLHGSTILYADIYTPLIDMVNNPHKYGF 300

Query: 301 EHTNRGCCGTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVYKSITESLLKQF 357
           EHTN GCCGTGLAE  PLCNALTPTCED SKY+FWDS+HPTE+ YK +TE  LKQF
Sbjct: 301 EHTNTGCCGTGLAEVAPLCNALTPTCEDSSKYLFWDSVHPTEAAYKFMTELFLKQF 356

BLAST of CmoCh02G007130 vs. NCBI nr
Match: XP_022947811.1 (GDSL esterase/lipase At2g30310-like [Cucurbita moschata])

HSP 1 Score: 608.6 bits (1568), Expect = 3.4e-170
Identity = 297/356 (83.43%), Postives = 318/356 (89.33%), Query Frame = 0

Query: 1   MARVNYLIAALSLYIIWSLCIIKLGSTSDLKISRSFPAILIFGDSTVDTGNNNFISTIFK 60
           MARVNYLIAALSL I+WSLC+ KL S SDLK   SFPAILIFGDSTVDTGNNNFI TI K
Sbjct: 1   MARVNYLIAALSLKIVWSLCMSKLCSASDLKNFGSFPAILIFGDSTVDTGNNNFIPTITK 60

Query: 61  ANYSPYGKDFPGHMATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSKDDVKTGVSFAS 120
           ANYSPYGKDF GH+ATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLS DDVKTGV+FAS
Sbjct: 61  ANYSPYGKDFSGHIATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSNDDVKTGVNFAS 120

Query: 121 AGTGFDDLTATISKVIPVMKQIDLFKNYTQRLQRIAGVDESKKIIGSALVIISAGTNDLN 180
           AG+GFDDLT  ISKVIP+MKQ+D+FKNY QRL    GVDESKKIIGSALVI+SAGTND  
Sbjct: 121 AGSGFDDLTTMISKVIPMMKQVDMFKNYIQRLTESVGVDESKKIIGSALVILSAGTNDFT 180

Query: 181 INFYDIPTRKLQYNISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPVGCLPIQETVSF 240
            NFYD+P RKLQYNISGYQDFI  RLQ+FIKEIY LGCRNIVVAGLPP+GCLPIQET+SF
Sbjct: 181 KNFYDLPVRKLQYNISGYQDFIQERLQNFIKEIYDLGCRNIVVAGLPPIGCLPIQETISF 240

Query: 241 ENPLHRICLEDQNSDSKSYNQKLSKLLSNLQSQLPGSTMLYADIYTPLIDMINNPHQYGF 300
           + PL RICLEDQNSDS SYNQKLSKLL NLQSQL GST+LYADIYTPLIDM+NNPH+YGF
Sbjct: 241 QPPLKRICLEDQNSDSISYNQKLSKLLRNLQSQLHGSTILYADIYTPLIDMVNNPHKYGF 300

Query: 301 EHTNRGCCGTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVYKSITESLLKQF 357
           EHTN GCCGTGLAE  PLCNALTPTCED SKY+FWDS+HPTE+ YK +TE  LKQF
Sbjct: 301 EHTNTGCCGTGLAEVAPLCNALTPTCEDSSKYLFWDSVHPTEAAYKFMTELFLKQF 356

BLAST of CmoCh02G007130 vs. TAIR 10
Match: AT2G30310.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 397.1 bits (1019), Expect = 1.5e-110
Identity = 192/315 (60.95%), Postives = 236/315 (74.92%), Query Frame = 0

Query: 36  FPAILIFGDSTVDTGNNNFIS-TIFKANYSPYGKDFPGHMATGRFSNGKLIPDMVASRLG 95
           FPAILIFGDSTVDTGNNN+ S TIFKA + PYG D PGH A GR+SNGK+I D++AS+L 
Sbjct: 32  FPAILIFGDSTVDTGNNNYHSQTIFKAKHLPYGVDLPGHEANGRYSNGKVISDVIASKLN 91

Query: 96  IKELVPPFLDPKLSKDDVKTGVSFASAGTGFDDLTATISKVIPVMKQIDLFKNYTQRLQR 155
           IKELVPPFL P +S  D+ TGVSFASAG G+DD ++  SK IPV +Q  +FKNY  RL+ 
Sbjct: 92  IKELVPPFLQPNISHQDIVTGVSFASAGAGYDDRSSLSSKAIPVSQQPSMFKNYIARLKG 151

Query: 156 IAGVDESKKIIGSALVIISAGTNDLNINFYDIPTRKLQY-NISGYQDFILNRLQSFIKEI 215
           I G  ++ +II +ALV+ISAG ND  +NFYDIPTR+L+Y  I GYQ+FIL RL  F++E+
Sbjct: 152 IVGDKKAMEIINNALVVISAGPNDFILNFYDIPTRRLEYPTIHGYQEFILKRLDGFVREL 211

Query: 216 YQLGCRNIVVAGLPPVGCLPIQETVSFENPLHRICLEDQNSDSKSYNQKLSKLLSNLQSQ 275
           Y LGCRNIVV GLPP+GCLPIQ T    N L R C+E +N DS  YNQKL K L  +Q+ 
Sbjct: 212 YSLGCRNIVVGGLPPMGCLPIQMTAKMRNIL-RFCVEQENKDSVLYNQKLVKKLPEIQAS 271

Query: 276 LPGSTMLYADIYTPLIDMINNPHQYGFEHTNRGCCGTGLAEAGPLCNALTPTCEDPSKYI 335
           LPGS  LYA++Y PL+DMI NP +YGF+ T +GCCGTG  E   +CN LT TC + S ++
Sbjct: 272 LPGSNFLYANVYDPLMDMIQNPSKYGFKETKKGCCGTGYLETTFMCNPLTKTCPNHSDHL 331

Query: 336 FWDSIHPTESVYKSI 349
           FWDSIHP+E+ Y  I
Sbjct: 332 FWDSIHPSEAAYNYI 345

BLAST of CmoCh02G007130 vs. TAIR 10
Match: AT1G06990.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 391.0 bits (1003), Expect = 1.1e-108
Identity = 176/322 (54.66%), Postives = 236/322 (73.29%), Query Frame = 0

Query: 36  FPAILIFGDSTVDTGNNNFISTIFKANYSPYGKDFPGHMATGRFSNGKLIPDMVASRLGI 95
           FPAIL+FGDST+DTGNNN+I T  +AN+ PYG +FPGH ATGRFSNGKLIPD +AS +GI
Sbjct: 35  FPAILVFGDSTIDTGNNNYIKTYIRANFPPYGCNFPGHNATGRFSNGKLIPDFIASLMGI 94

Query: 96  KELVPPFLDPKLSKDDVKTGVSFASAGTGFDDLTATISKVIPVMKQIDLFKNYTQRLQRI 155
           K+ VPPFLDP LS  D+ TGV FASAG+G+D+LT   +  + V KQ D+ ++Y +RL +I
Sbjct: 95  KDTVPPFLDPHLSDSDIITGVCFASAGSGYDNLTDRATSTLSVDKQADMLRSYVERLSQI 154

Query: 156 AGVDESKKIIGSALVIISAGTNDLNINFYDIPTRKLQYNISGYQDFILNRLQSFIKEIYQ 215
            G +++  I+  ALVI+S+GTND N+N YD P+R+ +  + GYQ FIL+ + +F++E+Y 
Sbjct: 155 VGDEKAASIVSEALVIVSSGTNDFNLNLYDTPSRRQKLGVDGYQSFILSNVHNFVQELYD 214

Query: 216 LGCRNIVVAGLPPVGCLPIQETVSFENPLHRICLEDQNSDSKSYNQKLSKLLSNLQSQLP 275
           +GCR I+V GLPPVGCLPIQ T++ +    R C++ QNSDS+ +NQKL   L+ +QS L 
Sbjct: 215 IGCRKIMVLGLPPVGCLPIQMTMAMQKQNERRCIDKQNSDSQEFNQKLKNSLTEMQSNLT 274

Query: 276 GSTMLYADIYTPLIDMINNPHQYGFEHTNRGCCGTGLAEAGPLCNALTPTCEDPSKYIFW 335
           GS + Y DIY  L DM  NP +YG + T RGCCGTG  E   LCNALT  C +P++Y+FW
Sbjct: 275 GSVIFYGDIYGALFDMATNPQRYGLKETTRGCCGTGEIELAYLCNALTRICPNPNQYLFW 334

Query: 336 DSIHPTESVYKSITESLLKQFF 358
           D IHP++  Y  I+ SL++Q F
Sbjct: 335 DDIHPSQIAYIVISLSLVEQIF 356

BLAST of CmoCh02G007130 vs. TAIR 10
Match: AT2G30220.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 385.2 bits (988), Expect = 5.8e-107
Identity = 184/313 (58.79%), Postives = 231/313 (73.80%), Query Frame = 0

Query: 36  FPAILIFGDSTVDTGNNNFIS-TIFKANYSPYGKDFPGHMATGRFSNGKLIPDMVASRLG 95
           FPAILIFGDST DTGNNN+ S  +FKAN+ PYG D PGH A GRFSNGKLI D+++++L 
Sbjct: 31  FPAILIFGDSTADTGNNNYYSQAVFKANHLPYGVDLPGHEANGRFSNGKLISDVISTKLN 90

Query: 96  IKELVPPFLDPKLSKDDVKTGVSFASAGTGFDDLTATISKVIPVMKQIDLFKNYTQRLQR 155
           IKE VPPFL P +S  D+ TGV FASAG G+DD T+  SK IPV +Q  +FKNY  RL+ 
Sbjct: 91  IKEFVPPFLQPNISDQDIVTGVCFASAGAGYDDETSLSSKAIPVSQQPSMFKNYIARLKG 150

Query: 156 IAGVDESKKIIGSALVIISAGTNDLNINFYDIPTRKLQY-NISGYQDFILNRLQSFIKEI 215
           I G  ++ +II +ALV+ISAG ND  +NFYDIP R+L+Y  I GYQDF+L RL  F++E+
Sbjct: 151 IVGDKKAMEIINNALVVISAGPNDFILNFYDIPIRRLEYPTIYGYQDFVLKRLDGFVREL 210

Query: 216 YQLGCRNIVVAGLPPVGCLPIQETVSFENPLHRICLEDQNSDSKSYNQKLSKLLSNLQSQ 275
           Y LGCRNI+V GLPP+GCLPIQ T      L  IC+E +N DS  YNQKL K L  +Q+ 
Sbjct: 211 YSLGCRNILVGGLPPMGCLPIQLTAKLRTIL-GICVEQENKDSILYNQKLVKKLPEIQAS 270

Query: 276 LPGSTMLYADIYTPLIDMINNPHQYGFEHTNRGCCGTGLAEAGPLCNALTPTCEDPSKYI 335
           LPGS  LYA++Y P++DMI NP +YGF+ T +GCCGTG  E   LC +L+ TC + S ++
Sbjct: 271 LPGSKFLYANVYDPVMDMIRNPSKYGFKETKKGCCGTGYLETSFLCTSLSKTCPNHSDHL 330

Query: 336 FWDSIHPTESVYK 347
           FWDSIHP+E+ YK
Sbjct: 331 FWDSIHPSEAAYK 342

BLAST of CmoCh02G007130 vs. TAIR 10
Match: AT2G24560.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 375.2 bits (962), Expect = 6.0e-104
Identity = 182/337 (54.01%), Postives = 247/337 (73.29%), Query Frame = 0

Query: 15  IIWSLCIIKLGSTSDLKISRS----FPAILIFGDSTVDTGNNNFIS-TIFKANYSPYGKD 74
           I ++L I  L S+ D   + +    FPAILIFGDSTVDTGNNN+ S TIFKA + PYG D
Sbjct: 7   ITFTLFIAALLSSCDAATNATSQPLFPAILIFGDSTVDTGNNNYHSQTIFKAKHLPYGID 66

Query: 75  FPGHMATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSKDDVKTGVSFASAGTGFDDLT 134
            P H A+GRF+NGK+  D++A++L IK+ VPPFL P LS  ++ TGV FASAG G+DD T
Sbjct: 67  LPNHKASGRFTNGKIFSDIIATKLNIKQFVPPFLQPNLSDQEIVTGVCFASAGAGYDDHT 126

Query: 135 ATISKVIPVMKQIDLFKNYTQRLQRIAGVDESKKIIGSALVIISAGTNDLNINFYDIPTR 194
           +  ++ I V+ Q  +FKNY  RL+ I G  ++ +II +ALV+ISAG ND  +N+YDIP+R
Sbjct: 127 SLSTQAIRVLDQQKMFKNYIARLKSIVGDKKAMEIIKNALVVISAGPNDFILNYYDIPSR 186

Query: 195 KLQY-NISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPVGCLPIQETVSFENPLHRIC 254
           +L++ +ISGYQDF+L RL +F++E+Y LGCR I+V GLPP+GCLPIQ T  F N L R C
Sbjct: 187 RLEFPHISGYQDFVLQRLDNFVRELYSLGCRKIMVGGLPPMGCLPIQMTAKFRNAL-RFC 246

Query: 255 LEDQNSDSKSYNQKLSKLLSNLQSQLPGSTMLYADIYTPLIDMINNPHQYGFEHTNRGCC 314
           LE +N DS  YNQKL  LL  +++ L GS +LY+++Y P++DM+ NP +YGF+ T RGCC
Sbjct: 247 LEQENRDSVLYNQKLQNLLPQIEASLTGSKILYSNVYDPMMDMMQNPSKYGFKETKRGCC 306

Query: 315 GTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVY 346
           GTG  E   +CNA +PTC + S+++F+DSIHP+E+ Y
Sbjct: 307 GTGHLETSFMCNAFSPTCRNHSEFLFFDSIHPSEATY 342

BLAST of CmoCh02G007130 vs. TAIR 10
Match: AT2G31540.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 373.2 bits (957), Expect = 2.3e-103
Identity = 178/340 (52.35%), Postives = 245/340 (72.06%), Query Frame = 0

Query: 11  LSLYIIWSLCIIKLGSTSDLKISRSFPAILIFGDSTVDTGNNNF-ISTIFKANYSPYGKD 70
           L+L+I  +L +    + ++      FPAILIFGDSTVDTGNNN+ + TIF+A + PYG D
Sbjct: 9   LTLFIATTL-LAPCNAAANATTKPLFPAILIFGDSTVDTGNNNYPLPTIFRAEHFPYGMD 68

Query: 71  FPGHMATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSKDDVKTGVSFASAGTGFDDLT 130
            P   A GRFSNGKLI D++A++L IKE +PPFL P LS  D+ TGV FASAG G+DDLT
Sbjct: 69  LPDGKANGRFSNGKLISDIIATKLNIKEFIPPFLQPNLSDQDILTGVCFASAGAGYDDLT 128

Query: 131 ATISKVIPVMKQIDLFKNYTQRLQRIAGVDESKKIIGSALVIISAGTNDLNINFYDIPTR 190
           +  ++ I V +Q ++FK+Y  RL+ I G  ++ +II +A V++SAG ND  +N+Y+IP+R
Sbjct: 129 SLSTQAIRVSEQPNMFKSYIARLKGIVGDKKAMEIINNAFVVVSAGPNDFILNYYEIPSR 188

Query: 191 KLQYN-ISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPVGCLPIQETVSFENPLHRIC 250
           +L+Y  ISGYQDFIL RL++F++E+Y LG RN++V GLPP+GCLPI  T  F N + R C
Sbjct: 189 RLEYPFISGYQDFILKRLENFVRELYSLGVRNVLVGGLPPMGCLPIHMTAKFRN-IFRFC 248

Query: 251 LEDQNSDSKSYNQKLSKLLSNLQSQLPGSTMLYADIYTPLIDMINNPHQYGFEHTNRGCC 310
           LE  N DS  YN+KL  LL  +++ LPGS  LYAD+Y P+++MI NP +YGF+ T RGCC
Sbjct: 249 LEHHNKDSVLYNEKLQNLLPQIEASLPGSKFLYADVYNPMMEMIQNPSKYGFKETKRGCC 308

Query: 311 GTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVYKSI 349
           GTG  E   +CN  +P C++ S+++F+DSIHP+E+ Y  I
Sbjct: 309 GTGFLETSFMCNVFSPVCQNRSEFLFFDSIHPSEATYNVI 346

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O229272.1e-10960.95GDSL esterase/lipase At2g30310 OS=Arabidopsis thaliana OX=3702 GN=At2g30310 PE=2... [more]
Q9LMJ31.5e-10754.66GDSL esterase/lipase At1g06990 OS=Arabidopsis thaliana OX=3702 GN=At1g06990 PE=2... [more]
O229188.1e-10658.79GDSL esterase/lipase At2g30220 OS=Arabidopsis thaliana OX=3702 GN=At2g30220 PE=3... [more]
Q9SIQ22.9e-10352.62GDSL esterase/lipase At2g31550 OS=Arabidopsis thaliana OX=3702 GN=At2g31550 PE=3... [more]
Q9SJA98.4e-10354.01GDSL esterase/lipase At2g24560 OS=Arabidopsis thaliana OX=3702 GN=At2g24560 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1G7Z03.0e-204100.00GDSL esterase/lipase At2g30310-like OS=Cucurbita moschata OX=3662 GN=LOC11145158... [more]
A0A6J1KWV54.2e-19092.16GDSL esterase/lipase At2g30310-like OS=Cucurbita maxima OX=3661 GN=LOC111499482 ... [more]
A0A6J1L3737.5e-17183.71GDSL esterase/lipase At2g30310-like OS=Cucurbita maxima OX=3661 GN=LOC111499480 ... [more]
A0A6J1G7I31.7e-17083.43GDSL esterase/lipase At2g30310-like OS=Cucurbita moschata OX=3662 GN=LOC11145156... [more]
A0A6J1KWV16.3e-17083.43GDSL esterase/lipase At2g30310-like OS=Cucurbita maxima OX=3661 GN=LOC111499475 ... [more]
Match NameE-valueIdentityDescription
XP_022947820.16.2e-204100.00GDSL esterase/lipase At2g30310-like [Cucurbita moschata][more]
XP_023533087.12.4e-19596.64GDSL esterase/lipase At2g30310-like [Cucurbita pepo subsp. pepo][more]
XP_023006812.18.7e-19092.16GDSL esterase/lipase At2g30310-like [Cucurbita maxima][more]
XP_023006809.11.5e-17083.71GDSL esterase/lipase At2g30310-like [Cucurbita maxima][more]
XP_022947811.13.4e-17083.43GDSL esterase/lipase At2g30310-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT2G30310.11.5e-11060.95GDSL-like Lipase/Acylhydrolase family protein [more]
AT1G06990.11.1e-10854.66GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT2G30220.15.8e-10758.79GDSL-like Lipase/Acylhydrolase family protein [more]
AT2G24560.16.0e-10454.01GDSL-like Lipase/Acylhydrolase family protein [more]
AT2G31540.12.3e-10352.35GDSL-like Lipase/Acylhydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 39..350
e-value: 9.0E-35
score: 121.1
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 26..356
e-value: 1.6E-76
score: 259.8
NoneNo IPR availablePANTHERPTHR45642GDSL ESTERASE/LIPASE EXL3coord: 34..351
NoneNo IPR availablePANTHERPTHR45642:SF30GDSL-LIKE LIPASE/ACYLHYDROLASEcoord: 34..351
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 28..354
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 37..353
e-value: 5.22763E-146
score: 413.55

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh02G007130.1CmoCh02G007130.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016788 hydrolase activity, acting on ester bonds