CmoCh02G005470 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh02G005470
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	E3 ubiquitin-protein ligase RNF126-like
Location	Cmo_Chr02: 3105494 .. 3106162 (+)
RNA-Seq Expression	CmoCh02G005470
Synteny	CmoCh02G005470

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTTTAACCTTCGGCGACTCTTTGTCCTTCCAATTTTCCATCCGCACCAATACCACCGCCACCGTCGCTTCCCACGAGCAACTCATGCAGTTCGTGGAACTCCGACTCGGACATTCGAGCCATCTCATCTCTCGATCTTCTCGCCAAATTCTTCAACAAAGTCCCTCAACTCCATTGGGCGAATCTCTGTTCCCTTTTATTCTGCGCGACCTGGAACACCCACCCTTCCTCCCCAACTACCATGTGCTTCATTTTTTGTCTTCCTTCAGCATCCCCATTGCCGCCTGCGATATCATCTATCAGGAGATCTCCTCGTTCGCGCTCCGCTTGGCCGCCGACAACGTCGATCTCAACTTCCACATCGTCGTGGATGTGGATGCCATCGAAATGATATGGGTGGACCTGGACCCATTCCTTGTTGAAGAATATTCTCCGCCTGCGATGAACGGGGCGCCGGCGTCGGCGATTGAGAGATTGCAGAGGCAGAAATTTGATGGGTTGAGGGAGGAAGAGGAGGGGGATTGCAGTGTCTGTTGCGAGGCGCTGAAGGGAGAAGAGGAGGTGAGCAGAATCCCGTGTGGTCATGTGTATCACAAATGCTGTATCCTCAAGTGGCTTCAGATGAGCAATTCATGTCCTCTCTGTAGAACCAAATTAGAACAATAA

mRNA sequence

ATGTCTTTAACCTTCGGCGACTCTTTGTCCTTCCAATTTTCCATCCGCACCAATACCACCGCCACCGTCGCTTCCCACGAGCAACTCATGCAGTTCGTGGAACTCCGACTCGGACATTCGAGCCATCTCATCTCTCGATCTTCTCGCCAAATTCTTCAACAAAGTCCCTCAACTCCATTGGGCGAATCTCTGTTCCCTTTTATTCTGCGCGACCTGGAACACCCACCCTTCCTCCCCAACTACCATGTGCTTCATTTTTTGTCTTCCTTCAGCATCCCCATTGCCGCCTGCGATATCATCTATCAGGAGATCTCCTCGTTCGCGCTCCGCTTGGCCGCCGACAACGTCGATCTCAACTTCCACATCGTCGTGGATGTGGATGCCATCGAAATGATATGGGTGGACCTGGACCCATTCCTTGTTGAAGAATATTCTCCGCCTGCGATGAACGGGGCGCCGGCGTCGGCGATTGAGAGATTGCAGAGGCAGAAATTTGATGGGTTGAGGGAGGAAGAGGAGGGGGATTGCAGTGTCTGTTGCGAGGCGCTGAAGGGAGAAGAGGAGGTGAGCAGAATCCCGTGTGGTCATGTGTATCACAAATGCTGTATCCTCAAGTGGCTTCAGATGAGCAATTCATGTCCTCTCTGTAGAACCAAATTAGAACAATAA

Coding sequence (CDS)

ATGTCTTTAACCTTCGGCGACTCTTTGTCCTTCCAATTTTCCATCCGCACCAATACCACCGCCACCGTCGCTTCCCACGAGCAACTCATGCAGTTCGTGGAACTCCGACTCGGACATTCGAGCCATCTCATCTCTCGATCTTCTCGCCAAATTCTTCAACAAAGTCCCTCAACTCCATTGGGCGAATCTCTGTTCCCTTTTATTCTGCGCGACCTGGAACACCCACCCTTCCTCCCCAACTACCATGTGCTTCATTTTTTGTCTTCCTTCAGCATCCCCATTGCCGCCTGCGATATCATCTATCAGGAGATCTCCTCGTTCGCGCTCCGCTTGGCCGCCGACAACGTCGATCTCAACTTCCACATCGTCGTGGATGTGGATGCCATCGAAATGATATGGGTGGACCTGGACCCATTCCTTGTTGAAGAATATTCTCCGCCTGCGATGAACGGGGCGCCGGCGTCGGCGATTGAGAGATTGCAGAGGCAGAAATTTGATGGGTTGAGGGAGGAAGAGGAGGGGGATTGCAGTGTCTGTTGCGAGGCGCTGAAGGGAGAAGAGGAGGTGAGCAGAATCCCGTGTGGTCATGTGTATCACAAATGCTGTATCCTCAAGTGGCTTCAGATGAGCAATTCATGTCCTCTCTGTAGAACCAAATTAGAACAATAA

Protein sequence

MSLTFGDSLSFQFSIRTNTTATVASHEQLMQFVELRLGHSSHLISRSSRQILQQSPSTPLGESLFPFILRDLEHPPFLPNYHVLHFLSSFSIPIAACDIIYQEISSFALRLAADNVDLNFHIVVDVDAIEMIWVDLDPFLVEEYSPPAMNGAPASAIERLQRQKFDGLREEEEGDCSVCCEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ

Homology

[Close]

BLAST of CmoCh02G005470 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 4.0e-10
Identity = 31/76 (40.79%), Postives = 43/76 (56.58%), Query Frame = 0


Query: 145 SPPAMNGAPASAIERLQRQKFDGLREEEEGDCSVCCEALKGEEEVSRIPCGHVYHKCCIL 204
           +PPA      SAIE L R +        E +C+VC E  + E E   +PC H++H  CI+
Sbjct: 172 NPPASK----SAIESLPRVEISDCHIGSEANCAVCTEIFETETEAREMPCKHLFHDDCIV 231

Query: 205 KWLQMSNSCPLCRTKL 221
            WL + NSCP+CR +L
Sbjct: 232 PWLSIRNSCPVCRFEL 243

[Close]

BLAST of CmoCh02G005470 vs. ExPASy Swiss-Prot
Match: Q9SI09 (Probable E3 ubiquitin-protein ligase XERICO OS=Arabidopsis thaliana OX=3702 GN=XERICO PE=1 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 8.9e-10
Identity = 30/80 (37.50%), Postives = 45/80 (56.25%), Query Frame = 0


Query: 142 EEYSPPAMNGAPASAIERLQRQKFDGLREEEEGDCSVCCEALKGEEEVSRIPCGHVYHKC 201
           E Y     N  P    E L R K     ++ + +CSVC    +G+ E++++ CGH++HK 
Sbjct: 74  ESYLEEFRNRTPTLRFESLCRCK-----KQADNECSVCLSKFQGDSEINKLKCGHLFHKT 133

Query: 202 CILKWLQMSN-SCPLCRTKL 221
           C+ KW+   N +CPLCRT L
Sbjct: 134 CLEKWIDYWNITCPLCRTPL 148

[Close]

BLAST of CmoCh02G005470 vs. ExPASy Swiss-Prot
Match: Q9SNB6 (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.2e-09
Identity = 30/76 (39.47%), Postives = 43/76 (56.58%), Query Frame = 0


Query: 145 SPPAMNGAPASAIERLQRQKFDGLREEEEGDCSVCCEALKGEEEVSRIPCGHVYHKCCIL 204
           +PPA      SAIE L R +      + E +C+VC E  +   E   +PC H++H  CI+
Sbjct: 188 NPPASK----SAIESLPRVEISDCHTKAEANCAVCTEVFEAGIEGREMPCKHIFHGDCIV 247

Query: 205 KWLQMSNSCPLCRTKL 221
            WL + NSCP+CR +L
Sbjct: 248 PWLSIRNSCPVCRFEL 259

[Close]

BLAST of CmoCh02G005470 vs. ExPASy Swiss-Prot
Match: Q6ZSG1 (E3 ubiquitin-protein ligase RNF165 OS=Homo sapiens OX=9606 GN=RNF165 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.2e-09
Identity = 30/85 (35.29%), Postives = 49/85 (57.65%), Query Frame = 0


Query: 151 GAPASAIERL-------QRQKFDGLREEEEGD-------CSVCCEALKGEEEVSRIPCGH 210
           GA  + IER        +R+  DG  +++EG+       C++C   L+  E+V R+PC H
Sbjct: 255 GAVQNTIERFTFPHKYKKRRPQDGKGKKDEGEESDTDEKCTICLSMLEDGEDVRRLPCMH 314

Query: 211 VYHKCCILKWLQMSNSCPLCRTKLE 222
           ++H+ C+ +WL MS  CP+CR  +E
Sbjct: 315 LFHQLCVDQWLAMSKKCPICRVDIE 339

[Close]

BLAST of CmoCh02G005470 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 3.4e-09
Identity = 28/76 (36.84%), Postives = 45/76 (59.21%), Query Frame = 0


Query: 145 SPPAMNGAPASAIERLQRQKFDGLREEEEGDCSVCCEALKGEEEVSRIPCGHVYHKCCIL 204
           S P+ NG P +  E +  +    ++ EE   CSVC + L+   +  ++PC H +H  CIL
Sbjct: 170 SDPSRNGTPPAKKEAV--EALPTVKIEEVVSCSVCLDDLEVGSQAKQMPCEHKFHSSCIL 229

Query: 205 KWLQMSNSCPLCRTKL 221
            WL++ +SCP+CR +L
Sbjct: 230 PWLELHSSCPVCRFEL 243

[Close]

BLAST of CmoCh02G005470 vs. ExPASy TrEMBL
Match: A0A6J1L387 (RING-H2 finger protein ATL16-like OS=Cucurbita maxima OX=3661 GN=LOC111499493 PE=4 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 1.3e-117
Identity = 214/223 (95.96%), Postives = 218/223 (97.76%), Query Frame = 0


Query: 1   MSLTFGDSLSFQFSIRTNTTATVASHEQLMQFVELRLGHSSHLISRSSRQILQQSPSTPL 60
           MSLTFGDSLSFQFSIRTNTT T+ASHE  MQFVELRLGHSSHLISRSSRQILQQSPSTPL
Sbjct: 1   MSLTFGDSLSFQFSIRTNTTGTLASHEHPMQFVELRLGHSSHLISRSSRQILQQSPSTPL 60

Query: 61  GESLFPFILRDLEHPPFLPNYHVLHFLSSFSIPIAACDIIYQEISSFALRLAADNVDLNF 120
           GESLFPFILRDLEHPPFLPNYHVLHFLSSFSIP+AACDIIYQEISSFALRL ADNVDLNF
Sbjct: 61  GESLFPFILRDLEHPPFLPNYHVLHFLSSFSIPVAACDIIYQEISSFALRLTADNVDLNF 120

Query: 121 HIVVDVDAIEMIWVDLDPFLVEEYSPPAMNGAPASAIERLQRQKFDGLR-EEEEGDCSVC 180
           HIVVDVDAIEMIWVDLDPFLVEEYSPPAMNGAPASAIERLQRQKFDGLR EEEEGDCSVC
Sbjct: 121 HIVVDVDAIEMIWVDLDPFLVEEYSPPAMNGAPASAIERLQRQKFDGLREEEEEGDCSVC 180

Query: 181 CEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ 223
           CEAL+GEEEVSRIPCGHVYHKCCILKWLQ+SNSCPLCRTKLEQ
Sbjct: 181 CEALRGEEEVSRIPCGHVYHKCCILKWLQISNSCPLCRTKLEQ 223

[Close]

BLAST of CmoCh02G005470 vs. ExPASy TrEMBL
Match: A0A6J1G809 (probable E3 ubiquitin-protein ligase RHA4A OS=Cucurbita moschata OX=3662 GN=LOC111451600 PE=4 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 3.3e-108
Identity = 197/222 (88.74%), Postives = 209/222 (94.14%), Query Frame = 0


Query: 1   MSLTFGDSLSFQFSIRTNTTATVASHEQLMQFVELRLGHSSHLISRSSRQILQQSPSTPL 60
           MSLTFGDSLSFQ S RTN+T T+ASHEQ MQFVELRLGHSSHLISRSSRQILQQ+PSTP+
Sbjct: 1   MSLTFGDSLSFQCSFRTNSTGTLASHEQPMQFVELRLGHSSHLISRSSRQILQQTPSTPM 60

Query: 61  GESLFPFILRDLEHPPFLPNYHVLHFLSSFSIPIAACDIIYQEISSFALRLAADNVDLNF 120
           G++LFPFILRDL HPPFL   HVL FLSSFSIPIA C+II+ EISSFAL LAA NVDLNF
Sbjct: 61  GDALFPFILRDLLHPPFLSKSHVLRFLSSFSIPIAVCNIIFVEISSFALHLAAGNVDLNF 120

Query: 121 HIVVDVDAIEMIWVDLDPFLVEEYSPPAMNGAPASAIERLQRQKFDGLREEEEGDCSVCC 180
           HIVVDVDAIEMIWVDLDPFL+EEYSPPAMNGAPASAIERLQRQ+FDGLREEEEG+CSVCC
Sbjct: 121 HIVVDVDAIEMIWVDLDPFLIEEYSPPAMNGAPASAIERLQRQEFDGLREEEEGECSVCC 180

Query: 181 EALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ 223
           EALKGEEEVSRIPCGHVYHKCCIL+WLQMSNSCPLCRTKLEQ
Sbjct: 181 EALKGEEEVSRIPCGHVYHKCCILRWLQMSNSCPLCRTKLEQ 222

[Close]

BLAST of CmoCh02G005470 vs. ExPASy TrEMBL
Match: A0A6J1G7Z5 (E3 ubiquitin-protein ligase RNF126-like OS=Cucurbita moschata OX=3662 GN=LOC111451666 PE=4 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 1.1e-100
Identity = 187/223 (83.86%), Postives = 205/223 (91.93%), Query Frame = 0


Query: 1   MSLTFGDSLSFQFSIRTNTTATVASHEQLMQFVELRLGHSSHLISRSSRQILQQSPSTPL 60
           MSLTF D LSFQ SI++    TVAS +Q MQ +ELRLG+S+HLISRSSRQILQQ+P+TP+
Sbjct: 1   MSLTFHDYLSFQCSIQSTCNGTVASDQQPMQLLELRLGNSTHLISRSSRQILQQTPTTPM 60

Query: 61  GESLFPFILRDLEHPPFLPNYHVLHFLSSFSIPIAACDIIYQEISSFALRLAADNVDLNF 120
           G++LFPF+LRDL+HPPFL N +VL FLSSFSIPIAACDIIY+EISSFAL+LAA NVDLNF
Sbjct: 61  GDALFPFVLRDLQHPPFLSNCYVLQFLSSFSIPIAACDIIYEEISSFALQLAAGNVDLNF 120

Query: 121 HIVVDVDAIEMIWVDLDPFLVEEYSPPAMNGAPASAIERLQRQKFDGLR-EEEEGDCSVC 180
           HIVV+VD IEMIWVDLDPFLVEEYSP  MNGAPASAIERLQRQ+FDGLR EEEEGDCSVC
Sbjct: 121 HIVVEVDVIEMIWVDLDPFLVEEYSPAEMNGAPASAIERLQRQEFDGLREEEEEGDCSVC 180

Query: 181 CEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ 223
           CEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ
Sbjct: 181 CEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ 223

[Close]

BLAST of CmoCh02G005470 vs. ExPASy TrEMBL
Match: A0A6J1KYV1 (E3 ubiquitin-protein ligase RNF126-like OS=Cucurbita maxima OX=3661 GN=LOC111499496 PE=4 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 2.8e-99
Identity = 186/223 (83.41%), Postives = 203/223 (91.03%), Query Frame = 0


Query: 1   MSLTFGDSLSFQFSIRTNTTATVASHEQLMQFVELRLGHSSHLISRSSRQILQQSPSTPL 60
           MSLTF D LSFQ SI++    TVAS +Q +Q +ELRLGHS+HLISRSSRQILQQ+PSTP+
Sbjct: 1   MSLTFHDYLSFQCSIQSTCNGTVASGQQPIQLLELRLGHSTHLISRSSRQILQQTPSTPI 60

Query: 61  GESLFPFILRDLEHPPFLPNYHVLHFLSSFSIPIAACDIIYQEISSFALRLAADNVDLNF 120
           G++LFPF+LRDL+HPPFL N +VL FLSSFSIPIAACDIIY+EISSFAL+LAA N+DLNF
Sbjct: 61  GDALFPFVLRDLQHPPFLSNCYVLQFLSSFSIPIAACDIIYEEISSFALQLAAGNMDLNF 120

Query: 121 HIVVDVDAIEMIWVDLDPFLVEEYSPPAMNGAPASAIERLQRQKFDGLR-EEEEGDCSVC 180
           HIVV+VD  EMIWVDLDPFLVEEYSP  MNGAPASAIERLQRQKFDGLR EEEEGDCSVC
Sbjct: 121 HIVVEVDVNEMIWVDLDPFLVEEYSPAEMNGAPASAIERLQRQKFDGLREEEEEGDCSVC 180

Query: 181 CEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ 223
           CEALKGEEEVSRIPCGHVYHK CILKWLQMSNSCPLCRTKLEQ
Sbjct: 181 CEALKGEEEVSRIPCGHVYHKFCILKWLQMSNSCPLCRTKLEQ 223

[Close]

BLAST of CmoCh02G005470 vs. ExPASy TrEMBL
Match: A0A6J1G826 (E3 ubiquitin-protein ligase RNF38-like OS=Cucurbita moschata OX=3662 GN=LOC111451599 PE=4 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 1.5e-84
Identity = 162/223 (72.65%), Postives = 186/223 (83.41%), Query Frame = 0


Query: 1   MSLTFGDSLSFQFSIRTNTTATVASHEQLMQFVELRLGHSSHLISRSSRQILQQSPSTPL 60
           MSL+  D LS+Q S+R     ++ S EQ MQ +ELR+GHS +LISRSS QILQ+SPSTP+
Sbjct: 48  MSLSSHDYLSYQCSMRGTCNDSLGSDEQPMQLMELRVGHSRNLISRSSHQILQRSPSTPM 107

Query: 61  GESLFPFILRDLEHPPFLPNYHVLHFLSSFSIPIAACDIIYQEISSFALRLAADNVDLNF 120
           G++LF FILRD E PPFL N  VL FLSSF IPI AC++IY +ISSFA  + AD+V LNF
Sbjct: 108 GDALFSFILRDQEQPPFLENSSVLRFLSSFRIPIPACNVIYDQISSFAQHMTADDVHLNF 167

Query: 121 HIVVDVDAIEMIWVDLDPFLVEEYSPPAMNGAPASAIERLQRQKFDGLR-EEEEGDCSVC 180
           H+VVDV+  EM WVDLDPFLVEE SPP MNGAPASAIERLQRQ+F GLR EEEEGDCSVC
Sbjct: 168 HMVVDVNVSEMTWVDLDPFLVEENSPPPMNGAPASAIERLQRQEFGGLREEEEEGDCSVC 227

Query: 181 CEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ 223
           CEALKGEEE SRIPCGHVYHKCCILKWLQ+SNSCPLCRT+L++
Sbjct: 228 CEALKGEEEGSRIPCGHVYHKCCILKWLQISNSCPLCRTQLQE 270

[Close]

BLAST of CmoCh02G005470 vs. NCBI nr
Match: KAG6605228.1 (E3 ubiquitin-protein ligase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 442.2 bits (1136), Expect = 2.7e-120
Identity = 221/223 (99.10%), Postives = 221/223 (99.10%), Query Frame = 0


Query: 1   MSLTFGDSLSFQFSIRTNTTATVASHEQLMQFVELRLGHSSHLISRSSRQILQQSPSTPL 60
           MSLTFGDSLSFQFSIRTNTTATVASHEQLMQFVELRLGHSSHLISRSSRQILQQSPSTPL
Sbjct: 1   MSLTFGDSLSFQFSIRTNTTATVASHEQLMQFVELRLGHSSHLISRSSRQILQQSPSTPL 60

Query: 61  GESLFPFILRDLEHPPFLPNYHVLHFLSSFSIPIAACDIIYQEISSFALRLAADNVDLNF 120
           GESLFPFILRDLEHPPFLPNYHVLHFLSSFSIPIAACDIIYQEISSFALRLAADNVDLNF
Sbjct: 61  GESLFPFILRDLEHPPFLPNYHVLHFLSSFSIPIAACDIIYQEISSFALRLAADNVDLNF 120

Query: 121 HIVVDVDAIEMIWVDLDPFLVEEYSPPAMNGAPASAIERLQRQKFDGLR-EEEEGDCSVC 180
           HIVVDVDAIEMIWVDLDPFLVEEYSPP MNGAPASAIERLQRQKFDGLR EEEEGDCSVC
Sbjct: 121 HIVVDVDAIEMIWVDLDPFLVEEYSPPPMNGAPASAIERLQRQKFDGLREEEEEGDCSVC 180

Query: 181 CEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ 223
           CEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ
Sbjct: 181 CEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ 223

[Close]

BLAST of CmoCh02G005470 vs. NCBI nr
Match: XP_023006824.1 (RING-H2 finger protein ATL16-like [Cucurbita maxima])

HSP 1 Score: 432.2 bits (1110), Expect = 2.7e-117
Identity = 214/223 (95.96%), Postives = 218/223 (97.76%), Query Frame = 0


Query: 1   MSLTFGDSLSFQFSIRTNTTATVASHEQLMQFVELRLGHSSHLISRSSRQILQQSPSTPL 60
           MSLTFGDSLSFQFSIRTNTT T+ASHE  MQFVELRLGHSSHLISRSSRQILQQSPSTPL
Sbjct: 1   MSLTFGDSLSFQFSIRTNTTGTLASHEHPMQFVELRLGHSSHLISRSSRQILQQSPSTPL 60

Query: 61  GESLFPFILRDLEHPPFLPNYHVLHFLSSFSIPIAACDIIYQEISSFALRLAADNVDLNF 120
           GESLFPFILRDLEHPPFLPNYHVLHFLSSFSIP+AACDIIYQEISSFALRL ADNVDLNF
Sbjct: 61  GESLFPFILRDLEHPPFLPNYHVLHFLSSFSIPVAACDIIYQEISSFALRLTADNVDLNF 120

Query: 121 HIVVDVDAIEMIWVDLDPFLVEEYSPPAMNGAPASAIERLQRQKFDGLR-EEEEGDCSVC 180
           HIVVDVDAIEMIWVDLDPFLVEEYSPPAMNGAPASAIERLQRQKFDGLR EEEEGDCSVC
Sbjct: 121 HIVVDVDAIEMIWVDLDPFLVEEYSPPAMNGAPASAIERLQRQKFDGLREEEEEGDCSVC 180

Query: 181 CEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ 223
           CEAL+GEEEVSRIPCGHVYHKCCILKWLQ+SNSCPLCRTKLEQ
Sbjct: 181 CEALRGEEEVSRIPCGHVYHKCCILKWLQISNSCPLCRTKLEQ 223

[Close]

BLAST of CmoCh02G005470 vs. NCBI nr
Match: XP_022947840.1 (probable E3 ubiquitin-protein ligase RHA4A [Cucurbita moschata])

HSP 1 Score: 401.0 bits (1029), Expect = 6.8e-108
Identity = 197/222 (88.74%), Postives = 209/222 (94.14%), Query Frame = 0


Query: 1   MSLTFGDSLSFQFSIRTNTTATVASHEQLMQFVELRLGHSSHLISRSSRQILQQSPSTPL 60
           MSLTFGDSLSFQ S RTN+T T+ASHEQ MQFVELRLGHSSHLISRSSRQILQQ+PSTP+
Sbjct: 1   MSLTFGDSLSFQCSFRTNSTGTLASHEQPMQFVELRLGHSSHLISRSSRQILQQTPSTPM 60

Query: 61  GESLFPFILRDLEHPPFLPNYHVLHFLSSFSIPIAACDIIYQEISSFALRLAADNVDLNF 120
           G++LFPFILRDL HPPFL   HVL FLSSFSIPIA C+II+ EISSFAL LAA NVDLNF
Sbjct: 61  GDALFPFILRDLLHPPFLSKSHVLRFLSSFSIPIAVCNIIFVEISSFALHLAAGNVDLNF 120

Query: 121 HIVVDVDAIEMIWVDLDPFLVEEYSPPAMNGAPASAIERLQRQKFDGLREEEEGDCSVCC 180
           HIVVDVDAIEMIWVDLDPFL+EEYSPPAMNGAPASAIERLQRQ+FDGLREEEEG+CSVCC
Sbjct: 121 HIVVDVDAIEMIWVDLDPFLIEEYSPPAMNGAPASAIERLQRQEFDGLREEEEGECSVCC 180

Query: 181 EALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ 223
           EALKGEEEVSRIPCGHVYHKCCIL+WLQMSNSCPLCRTKLEQ
Sbjct: 181 EALKGEEEVSRIPCGHVYHKCCILRWLQMSNSCPLCRTKLEQ 222

[Close]

BLAST of CmoCh02G005470 vs. NCBI nr
Match: XP_023533113.1 (RING-H2 finger protein ATL1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 390.2 bits (1001), Expect = 1.2e-104
Identity = 195/223 (87.44%), Postives = 206/223 (92.38%), Query Frame = 0


Query: 1   MSLTFGDSLSFQFSIRTNTTATVASHEQLMQFVELRLGHSSHLISRSSRQILQQSPSTPL 60
           MSLTFGDSLSFQ S RTNTT  +ASHEQ MQFVELRLGHSSHLISRSSRQILQQ+PSTP+
Sbjct: 1   MSLTFGDSLSFQCSFRTNTTGNLASHEQPMQFVELRLGHSSHLISRSSRQILQQTPSTPM 60

Query: 61  GESLFPFILRDLEHPPFLPNYHVLHFLSSFSIPIAACDIIYQEISSFALRLAADNVDLNF 120
           G++LFPFILRDL  PPFL   HVL FLSSF+IPIA C+II+ EISSFAL LAA NVDLNF
Sbjct: 61  GDALFPFILRDLLDPPFLSKSHVLRFLSSFTIPIAVCNIIFAEISSFALHLAAGNVDLNF 120

Query: 121 HIVVDVDAIEMIWVDLDPFLVEEYSPPAMNGAPASAIERLQRQKFDGLREEE-EGDCSVC 180
           HIVVDVDAIEMIWVDLDPFL+EEYSP AMNGAPASAIERLQRQKFDGLREEE EGDCSVC
Sbjct: 121 HIVVDVDAIEMIWVDLDPFLIEEYSPAAMNGAPASAIERLQRQKFDGLREEEKEGDCSVC 180

Query: 181 CEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ 223
           CEALKGEEEVSRIPCGHVYHKCCILKWL+MSNSCPLCRT+LEQ
Sbjct: 181 CEALKGEEEVSRIPCGHVYHKCCILKWLEMSNSCPLCRTQLEQ 223

[Close]

BLAST of CmoCh02G005470 vs. NCBI nr
Match: KAG6605225.1 (E3 ubiquitin-protein ligase RDUF2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 389.0 bits (998), Expect = 2.7e-104
Identity = 193/222 (86.94%), Postives = 204/222 (91.89%), Query Frame = 0


Query: 1   MSLTFGDSLSFQFSIRTNTTATVASHEQLMQFVELRLGHSSHLISRSSRQILQQSPSTPL 60
           MSLTFGDSLSFQ S RTNTTATVASHEQ MQ VELRLGHSSHLISRSSRQILQQ+PSTP+
Sbjct: 1   MSLTFGDSLSFQCSFRTNTTATVASHEQPMQLVELRLGHSSHLISRSSRQILQQTPSTPM 60

Query: 61  GESLFPFILRDLEHPPFLPNYHVLHFLSSFSIPIAACDIIYQEISSFALRLAADNVDLNF 120
           G++LFPF LRDL HPPFL   HVL F+SSFSIPIAAC+II+ EISSFAL LAA NV  N 
Sbjct: 61  GDALFPFFLRDLLHPPFLSKSHVLRFMSSFSIPIAACNIIFAEISSFALHLAAGNVHRNL 120

Query: 121 HIVVDVDAIEMIWVDLDPFLVEEYSPPAMNGAPASAIERLQRQKFDGLREEEEGDCSVCC 180
           HIVVDV+ IEMIWVDLD FLVEEYSPPAMNGAPASAIERLQRQ+FDGLREEEEGDCSVCC
Sbjct: 121 HIVVDVNVIEMIWVDLDTFLVEEYSPPAMNGAPASAIERLQRQEFDGLREEEEGDCSVCC 180

Query: 181 EALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ 223
           EALKGEEEVSRIPCGHVYHKCCILKWLQM+NSCPLCRT+LEQ
Sbjct: 181 EALKGEEEVSRIPCGHVYHKCCILKWLQMNNSCPLCRTQLEQ 222

[Close]

BLAST of CmoCh02G005470 vs. TAIR 10
Match: AT5G59550.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 66.6 bits (161), Expect = 2.8e-11
Identity = 31/76 (40.79%), Postives = 43/76 (56.58%), Query Frame = 0


Query: 145 SPPAMNGAPASAIERLQRQKFDGLREEEEGDCSVCCEALKGEEEVSRIPCGHVYHKCCIL 204
           +PPA      SAIE L R +        E +C+VC E  + E E   +PC H++H  CI+
Sbjct: 172 NPPASK----SAIESLPRVEISDCHIGSEANCAVCTEIFETETEAREMPCKHLFHDDCIV 231

Query: 205 KWLQMSNSCPLCRTKL 221
            WL + NSCP+CR +L
Sbjct: 232 PWLSIRNSCPVCRFEL 243

[Close]

BLAST of CmoCh02G005470 vs. TAIR 10
Match: AT2G04240.1 (RING/U-box superfamily protein )

HSP 1 Score: 65.5 bits (158), Expect = 6.3e-11
Identity = 30/80 (37.50%), Postives = 45/80 (56.25%), Query Frame = 0


Query: 142 EEYSPPAMNGAPASAIERLQRQKFDGLREEEEGDCSVCCEALKGEEEVSRIPCGHVYHKC 201
           E Y     N  P    E L R K     ++ + +CSVC    +G+ E++++ CGH++HK 
Sbjct: 74  ESYLEEFRNRTPTLRFESLCRCK-----KQADNECSVCLSKFQGDSEINKLKCGHLFHKT 133

Query: 202 CILKWLQMSN-SCPLCRTKL 221
           C+ KW+   N +CPLCRT L
Sbjct: 134 CLEKWIDYWNITCPLCRTPL 148

[Close]

BLAST of CmoCh02G005470 vs. TAIR 10
Match: AT2G04240.2 (RING/U-box superfamily protein )

HSP 1 Score: 65.5 bits (158), Expect = 6.3e-11
Identity = 30/80 (37.50%), Postives = 45/80 (56.25%), Query Frame = 0


Query: 142 EEYSPPAMNGAPASAIERLQRQKFDGLREEEEGDCSVCCEALKGEEEVSRIPCGHVYHKC 201
           E Y     N  P    E L R K     ++ + +CSVC    +G+ E++++ CGH++HK 
Sbjct: 74  ESYLEEFRNRTPTLRFESLCRCK-----KQADNECSVCLSKFQGDSEINKLKCGHLFHKT 133

Query: 202 CILKWLQMSN-SCPLCRTKL 221
           C+ KW+   N +CPLCRT L
Sbjct: 134 CLEKWIDYWNITCPLCRTPL 148

[Close]

BLAST of CmoCh02G005470 vs. TAIR 10
Match: AT3G46620.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 65.1 bits (157), Expect = 8.2e-11
Identity = 30/76 (39.47%), Postives = 43/76 (56.58%), Query Frame = 0


Query: 145 SPPAMNGAPASAIERLQRQKFDGLREEEEGDCSVCCEALKGEEEVSRIPCGHVYHKCCIL 204
           +PPA      SAIE L R +      + E +C+VC E  +   E   +PC H++H  CI+
Sbjct: 188 NPPASK----SAIESLPRVEISDCHTKAEANCAVCTEVFEAGIEGREMPCKHIFHGDCIV 247

Query: 205 KWLQMSNSCPLCRTKL 221
            WL + NSCP+CR +L
Sbjct: 248 PWLSIRNSCPVCRFEL 259

[Close]

BLAST of CmoCh02G005470 vs. TAIR 10
Match: AT5G67120.1 (RING/U-box superfamily protein )

HSP 1 Score: 64.3 bits (155), Expect = 1.4e-10
Identity = 26/63 (41.27%), Postives = 40/63 (63.49%), Query Frame = 0


Query: 158 ERLQRQKFDGLREEEEGDCSVCCEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCR 217
           E L R+K++   ++E   C +C + LK  EEVS++ CGH +H  CI  WL ++N CPLC 
Sbjct: 209 ENLIRRKYEKRSDDETKRCVICQQKLKDNEEVSKLGCGHDFHFGCIKNWLMVTNKCPLCN 268

Query: 218 TKL 221
            ++
Sbjct: 269 REV 271

The following BLAST results are available for this feature:

BLAST of CmoCh02G005470 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q940T5	4.0e-10	40.79	E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ...	[more]
Q9SI09	8.9e-10	37.50	Probable E3 ubiquitin-protein ligase XERICO OS=Arabidopsis thaliana OX=3702 GN=X...	[more]
Q9SNB6	1.2e-09	39.47	E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 ...	[more]
Q6ZSG1	1.2e-09	35.29	E3 ubiquitin-protein ligase RNF165 OS=Homo sapiens OX=9606 GN=RNF165 PE=1 SV=1	[more]
Q6AVN2	3.4e-09	36.84	E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI...	[more]

BLAST of CmoCh02G005470 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1L387	1.3e-117	95.96	RING-H2 finger protein ATL16-like OS=Cucurbita maxima OX=3661 GN=LOC111499493 PE...	[more]
A0A6J1G809	3.3e-108	88.74	probable E3 ubiquitin-protein ligase RHA4A OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1G7Z5	1.1e-100	83.86	E3 ubiquitin-protein ligase RNF126-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1KYV1	2.8e-99	83.41	E3 ubiquitin-protein ligase RNF126-like OS=Cucurbita maxima OX=3661 GN=LOC111499...	[more]
A0A6J1G826	1.5e-84	72.65	E3 ubiquitin-protein ligase RNF38-like OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]

BLAST of CmoCh02G005470 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6605228.1	2.7e-120	99.10	E3 ubiquitin-protein ligase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023006824.1	2.7e-117	95.96	RING-H2 finger protein ATL16-like [Cucurbita maxima]	[more]
XP_022947840.1	6.8e-108	88.74	probable E3 ubiquitin-protein ligase RHA4A [Cucurbita moschata]	[more]
XP_023533113.1	1.2e-104	87.44	RING-H2 finger protein ATL1-like [Cucurbita pepo subsp. pepo]	[more]
KAG6605225.1	2.7e-104	86.94	E3 ubiquitin-protein ligase RDUF2, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of CmoCh02G005470 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G59550.1	2.8e-11	40.79	zinc finger (C3HC4-type RING finger) family protein	[more]
AT2G04240.1	6.3e-11	37.50	RING/U-box superfamily protein	[more]
AT2G04240.2	6.3e-11	37.50	RING/U-box superfamily protein	[more]
AT3G46620.1	8.2e-11	39.47	zinc finger (C3HC4-type RING finger) family protein	[more]
AT5G67120.1	1.4e-10	41.27	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 176..216 e-value: 6.1E-6 score: 35.7
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 175..217 e-value: 8.7E-12 score: 45.2
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 176..217 score: 12.902362
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 142..222 e-value: 1.5E-19 score: 71.5
None	No IPR available	PANTHER	PTHR45931	SI:CH211-59O9.10	coord: 85..221
None	No IPR available	PANTHER	PTHR45931:SF9	E3 UBIQUITIN-PROTEIN LIGASE RNF12-B-LIKE	coord: 85..221
None	No IPR available	CDD	cd16454	RING-H2_PA-TM-RING	coord: 175..217 e-value: 1.14954E-15 score: 66.5565
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 157..220

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh02G005470.1	CmoCh02G005470.1	mRNA