CmoCh02G000520 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh02G000520
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	heat stress transcription factor A-4d-like
Location	Cmo_Chr02: 279098 .. 280430 (+)
RNA-Seq Expression	CmoCh02G000520
Synteny	CmoCh02G000520

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATCCTTCTCATTGCTTCATCAATCCGCAAAATTTCCCTTCAACTTCTCGTTCTGTTCTTACCAGATTTCCCTTTCCCCTGTTTTTCTGGTTACATCACGGTGGCGACAACGGCGGCGCTGGCTGAAACTCCTACTGTTCAGTCAAATAAATGGAGGGCTCAGATGGAAGCTATGGCGGCGCACCGCCGCCATTTCTGACCAAAACATACGAGATGGTGGATGATCCGATGACCAACTCCGTCGTGTCATGGAGTAAAAGTGGTTACAGCTTCGTGGTTTGGAACCCACCGGAATTCGCCAAAGAATTGCTCCCAGTTTATTTCAAACACAACAATTTCTCTAGCTTCGTTCGTCAATTGAACACTTACGTAAGTTCCAACATCATAAAAACATCATATTCCAAAATCAAATCATCTTTTTCCCTGACATTGATTCCTCTGTTTTTTTAGTAAAAATCCTGAGAGAAAAATTCTGGTATCTCTTAATAGGGGTTCAGGAAGGTAGATCGAGATCAATGGGAATTTGCTAACGAGGGGTTCATAAGAGGACGAACCCATCTTCTAAAAAGCATCCATAGACGCAAACCAATCTTCAGCCATAGCCAGAGCCAGAGCCAGAGCCATGGCAGTGGAGCTCCATTGTCCGAGCTGGAGAGGCAAGAACTGGAGCTAAAAATCAAAACCCTTCATCAAGAAAAGACCATTCTCCAAACCCAGCTGCAAGAACACGAAAATGAAAAGGAACAAATCGGGCGTCAAATTCAAACAATGTGTCAGCAGATATGGCGAATGGGGAACCAACAGAAGCAGCTGATAGCAATAATGGCGGCGGAGTTGCAGAAGGATCAGTCGAGGAAAAGGAGAAAGATAGGGAAATTGAGTGAGTTTTTGGGTGAGGAATGGTTGGAAGTTGAGAGAGATGAGAGGAATGGGTTGAAGAATGGTTTGGAGGTTCCGGCATTGGAGCTGATGGGGAAGCTGGAAGTGTCGTTGGGGTTGTGTGAGGATTTGCTGTGCAACGTGGCGGAGGTTCTGGGCGGAGAGATGAGCGGGAAGAGGAAGGAAATGGAGGGTAAGGGTGTGAAAGAAGGGGAAAAGAGAGGGGAAAATGGAGTGAATGATGTGTTTTGGGAACAGTTTTTGACAGAGGTTCCGGGGTGTTCCAATGGTGGGGAAGTTTATTTGGATAGAAGGAGTAATGTTTTAAGGTAGATTAGGGGTTTGTAAATGATTTAGATTTCCTTCTTTGTTGTTCACATTACAATTCATTACCATTCTCTCCGGGAAAGGTTCAAATTTTATGTTGCGTATCTTCTTTATATATAAAACGGGA

mRNA sequence

AATCCTTCTCATTGCTTCATCAATCCGCAAAATTTCCCTTCAACTTCTCGTTCTGTTCTTACCAGATTTCCCTTTCCCCTGTTTTTCTGGTTACATCACGGTGGCGACAACGGCGGCGCTGGCTGAAACTCCTACTGTTCAGTCAAATAAATGGAGGGCTCAGATGGAAGCTATGGCGGCGCACCGCCGCCATTTCTGACCAAAACATACGAGATGGTGGATGATCCGATGACCAACTCCGTCGTGTCATGGAGTAAAAGTGGTTACAGCTTCGTGGTTTGGAACCCACCGGAATTCGCCAAAGAATTGCTCCCAGTTTATTTCAAACACAACAATTTCTCTAGCTTCGTTCGTCAATTGAACACTTACGGGTTCAGGAAGGTAGATCGAGATCAATGGGAATTTGCTAACGAGGGGTTCATAAGAGGACGAACCCATCTTCTAAAAAGCATCCATAGACGCAAACCAATCTTCAGCCATAGCCAGAGCCAGAGCCAGAGCCATGGCAGTGGAGCTCCATTGTCCGAGCTGGAGAGGCAAGAACTGGAGCTAAAAATCAAAACCCTTCATCAAGAAAAGACCATTCTCCAAACCCAGCTGCAAGAACACGAAAATGAAAAGGAACAAATCGGGCGTCAAATTCAAACAATGTGTCAGCAGATATGGCGAATGGGGAACCAACAGAAGCAGCTGATAGCAATAATGGCGGCGGAGTTGCAGAAGGATCAGTCGAGGAAAAGGAGAAAGATAGGGAAATTGAGTGAGTTTTTGGGTGAGGAATGGTTGGAAGTTGAGAGAGATGAGAGGAATGGGTTGAAGAATGGTTTGGAGGTTCCGGCATTGGAGCTGATGGGGAAGCTGGAAGTGTCGTTGGGGTTGTGTGAGGATTTGCTGTGCAACGTGGCGGAGGTTCTGGGCGGAGAGATGAGCGGGAAGAGGAAGGAAATGGAGGGTAAGGGTGTGAAAGAAGGGGAAAAGAGAGGGGAAAATGGAGTGAATGATGTGTTTTGGGAACAGTTTTTGACAGAGGTTCCGGGGTGTTCCAATGGTGGGGAAGTTTATTTGGATAGAAGGAGTAATGTTTTAAGGTAGATTAGGGGTTTGTAAATGATTTAGATTTCCTTCTTTGTTGTTCACATTACAATTCATTACCATTCTCTCCGGGAAAGGTTCAAATTTTATGTTGCGTATCTTCTTTATATATAAAACGGGA

Coding sequence (CDS)

ATGGAGGGCTCAGATGGAAGCTATGGCGGCGCACCGCCGCCATTTCTGACCAAAACATACGAGATGGTGGATGATCCGATGACCAACTCCGTCGTGTCATGGAGTAAAAGTGGTTACAGCTTCGTGGTTTGGAACCCACCGGAATTCGCCAAAGAATTGCTCCCAGTTTATTTCAAACACAACAATTTCTCTAGCTTCGTTCGTCAATTGAACACTTACGGGTTCAGGAAGGTAGATCGAGATCAATGGGAATTTGCTAACGAGGGGTTCATAAGAGGACGAACCCATCTTCTAAAAAGCATCCATAGACGCAAACCAATCTTCAGCCATAGCCAGAGCCAGAGCCAGAGCCATGGCAGTGGAGCTCCATTGTCCGAGCTGGAGAGGCAAGAACTGGAGCTAAAAATCAAAACCCTTCATCAAGAAAAGACCATTCTCCAAACCCAGCTGCAAGAACACGAAAATGAAAAGGAACAAATCGGGCGTCAAATTCAAACAATGTGTCAGCAGATATGGCGAATGGGGAACCAACAGAAGCAGCTGATAGCAATAATGGCGGCGGAGTTGCAGAAGGATCAGTCGAGGAAAAGGAGAAAGATAGGGAAATTGAGTGAGTTTTTGGGTGAGGAATGGTTGGAAGTTGAGAGAGATGAGAGGAATGGGTTGAAGAATGGTTTGGAGGTTCCGGCATTGGAGCTGATGGGGAAGCTGGAAGTGTCGTTGGGGTTGTGTGAGGATTTGCTGTGCAACGTGGCGGAGGTTCTGGGCGGAGAGATGAGCGGGAAGAGGAAGGAAATGGAGGGTAAGGGTGTGAAAGAAGGGGAAAAGAGAGGGGAAAATGGAGTGAATGATGTGTTTTGGGAACAGTTTTTGACAGAGGTTCCGGGGTGTTCCAATGGTGGGGAAGTTTATTTGGATAGAAGGAGTAATGTTTTAAGGTAG

Protein sequence

MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKHNNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQSHGSGAPLSELERQELELKIKTLHQEKTILQTQLQEHENEKEQIGRQIQTMCQQIWRMGNQQKQLIAIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDERNGLKNGLEVPALELMGKLEVSLGLCEDLLCNVAEVLGGEMSGKRKEMEGKGVKEGEKRGENGVNDVFWEQFLTEVPGCSNGGEVYLDRRSNVLR

Homology

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BLAST of CmoCh02G000520 vs. ExPASy Swiss-Prot
Match: Q93VB5 (Heat stress transcription factor A-4d OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA4D PE=1 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 2.0e-52
Identity = 126/278 (45.32%), Postives = 165/278 (59.35%), Query Frame = 0


Query: 3   GSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKHNN 62
           G  G  GG PPPFL KTYEMV+D  TN VVSW   G SFVVWNP +F+++LLP YFKHNN
Sbjct: 9   GGGGGGGGGPPPFLIKTYEMVEDAATNHVVSWGPGGASFVVWNPLDFSRDLLPKYFKHNN 68

Query: 63  FSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQSHGSGA 122
           FSSF+RQLNTYGFRK+D ++WEFANE FIRG THLLK+IHRRKP+ SHS  Q+Q +G   
Sbjct: 69  FSSFIRQLNTYGFRKIDPERWEFANEDFIRGHTHLLKNIHRRKPVHSHS-LQNQING--- 128

Query: 123 PLSELERQELELKIKTLHQEKTILQTQLQEHENEKEQIGRQIQTMCQQIWRMGNQQKQLI 182
           PL+E ER+ELE +I  L  EK+IL   LQ    ++  I  Q+Q M  ++  M  +QK ++
Sbjct: 129 PLAESERRELEEEINRLKYEKSILVADLQRQNQQQYVINWQMQAMEGRLVAMEQRQKNIV 188

Query: 183 AIMAAELQK------------DQSRKRRKIGKLSEFLGEEWLEVERDERNGLKNGLEVPA 242
           A +   LQ+            D   K+R++ K+  F+ +     E+        G + PA
Sbjct: 189 ASLCEMLQRRGGAVSSSLLESDHFSKKRRVPKMDLFVDDCAAGEEQKVFQFQGIGTDAPA 248

Query: 243 L---------ELMGKLEVSLGLCEDLL------CNVAE 254
           +         E   ++E+SL   E L       C  AE
Sbjct: 249 MPPVLPVTNGEAFDRVELSLVSLEKLFQRANDACTAAE 282

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BLAST of CmoCh02G000520 vs. ExPASy Swiss-Prot
Match: Q9FK72 (Heat stress transcription factor A-4c OS=Arabidopsis thaliana OX=3702 GN=HSFA4C PE=2 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 6.5e-51
Identity = 131/328 (39.94%), Postives = 187/328 (57.01%), Query Frame = 0


Query: 5   DGSYGGAP--PPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKHNN 64
           D + GG+   PPFLTKTYEMVDD  ++SVV+WS++  SF+V NP EF+++LLP +FKH N
Sbjct: 2   DENNGGSSSLPPFLTKTYEMVDDSSSDSVVAWSENNKSFIVKNPAEFSRDLLPRFFKHKN 61

Query: 65  FSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQSHGSGA 124
           FSSF+RQLNTYGFRKVD ++WEF N+ F+RGR +L+K+IHRRKP+ SHS    Q+     
Sbjct: 62  FSSFIRQLNTYGFRKVDPEKWEFLNDDFVRGRPYLMKNIHRRKPVHSHSLVNLQAQN--- 121

Query: 125 PLSELERQELELKIKTLHQEKTILQTQLQEHENEKEQIGRQIQTMCQQIWRMGNQQKQLI 184
           PL+E ER+ +E +I+ L  EK  L  +LQ  E E+++   Q+ T+  ++  M   QK ++
Sbjct: 122 PLTESERRSMEDQIERLKNEKEGLLAELQNQEQERKEFELQVTTLKDRLQHMEQHQKSIV 181

Query: 185 AIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDERNGLKNGLEVPA--LELMGKLEVS 244
           A ++           + +GK    L  E    ER +R   +N L   +  +E + KLE S
Sbjct: 182 AYVS-----------QVLGKPGLSLNLE--NHERRKRRFQENSLPPSSSHIEQVEKLESS 241

Query: 245 LGLCEDLLCNVAEVLGGEMSGKRKEMEGKGVKEGEKRGEN-------------------- 304
           L   E+L+    E  G + S    +     +  G+ R ++                    
Sbjct: 242 LTFWENLVSESCEKSGLQSSSMDHDAAESSLSIGDTRPKSSKIDMNSEPPVTVTAPAPKT 301

Query: 305 GVNDVFWEQFLTEVPGCSNGGEVYLDRR 309
           GVND FWEQ LTE PG +   EV  +RR
Sbjct: 302 GVNDDFWEQCLTENPGSTEQQEVQSERR 313

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BLAST of CmoCh02G000520 vs. ExPASy Swiss-Prot
Match: Q94J16 (Heat stress transcription factor A-4b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA4B PE=2 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 1.6e-49
Identity = 120/272 (44.12%), Postives = 172/272 (63.24%), Query Frame = 0


Query: 1   MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH 60
           MEG  G  GG+ PPFL+KTYEMVDDP T++VV W+ +G SFVV N PEF ++LLP YFKH
Sbjct: 1   MEGGGG--GGSLPPFLSKTYEMVDDPSTDAVVGWTPAGTSFVVANQPEFCRDLLPKYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQSHGS 120
           NNFSSFVRQLNTYGFRKVD +QWEFANE FI+G+ H LK+IHRRKPIFSHS   S S G+
Sbjct: 61  NNFSSFVRQLNTYGFRKVDPEQWEFANEDFIKGQRHRLKNIHRRKPIFSHS---SHSQGA 120

Query: 121 GAPLSELERQELELKIKTLHQEKTILQTQLQEHENEKEQIGRQIQTMCQQIWRMGNQQKQ 180
           G PL++ ER++ E +I+ L  +   L ++LQ +  +K  + +++Q + ++++ + +QQ+ 
Sbjct: 121 G-PLTDNERKDYEEEIERLKSDNAALSSELQNNTLKKLNMEKRMQALEEKLFVVEDQQRS 180

Query: 181 LIA-----------IMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDERNGLKNG-LEV 240
           LI+           + +   Q+D  RK+R++     F  +   +  +     L N   + 
Sbjct: 181 LISYVREIVKAPGFLSSFVQQQDHHRKKRRLPIPISFHEDANTQENQIMPCDLTNSPAQT 240

Query: 241 PALELMGKLEVSLGLCEDLLCNVAEVLGGEMS 261
              E   K+E SL   E+ L   +E  G ++S
Sbjct: 241 FYRESFDKMESSLNSLENFLREASEEFGNDIS 266

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BLAST of CmoCh02G000520 vs. ExPASy Swiss-Prot
Match: O49403 (Heat stress transcription factor A-4a OS=Arabidopsis thaliana OX=3702 GN=HSFA4A PE=1 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 6.1e-49
Identity = 123/367 (33.51%), Postives = 195/367 (53.13%), Query Frame = 0


Query: 2   EGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKHN 61
           E + G    + PPFLTKTYEMVDD  ++S+VSWS+S  SF+VWNPPEF+++LLP +FKHN
Sbjct: 3   ENNHGVSSSSLPPFLTKTYEMVDDSSSDSIVSWSQSNKSFIVWNPPEFSRDLLPRFFKHN 62

Query: 62  NFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQSHGSG 121
           NFSSF+RQLNTYGFRK D +QWEFAN+ F+RG+ HL+K+IHRRKP+ SHS    Q+  + 
Sbjct: 63  NFSSFIRQLNTYGFRKADPEQWEFANDDFVRGQPHLMKNIHRRKPVHSHSLPNLQAQLN- 122

Query: 122 APLSELERQELELKIKTLHQEKTILQTQLQEHENEKEQIGRQIQTMCQQIWRMGNQQKQL 181
            PL++ ER  +  +I+ L +EK  L  +L + + E+E    Q++ + +++  M  +QK +
Sbjct: 123 -PLTDSERVRMNNQIERLTKEKEGLLEELHKQDEEREVFEMQVKELKERLQHMEKRQKTM 182

Query: 182 IAIMAAELQK------------DQSRKRRKIGKLSEFLGEEWLEVERDERNGLKNGLEVP 241
           ++ ++  L+K            + + ++R+  ++  F  E  LE  +      + G   P
Sbjct: 183 VSFVSQVLEKPGLALNLSPCVPETNERKRRFPRIEFFPDEPMLEENKTCVVVREEGSTSP 242

Query: 242 ALEL----MGKLEVSLGLCEDLLCNVAEVLGGEMSGKRKEMEGKGV-------------- 301
           +       + +LE S+ + E+L+ +  E +    S    +++                  
Sbjct: 243 SSHTREHQVEQLESSIAIWENLVSDSCESMLQSRSMMTLDVDESSTFPESPPLSCIQLSV 302

Query: 302 ------------------KEGEKRGEN----------GVNDVFWEQFLTEVPGCSNGGEV 311
                              +G K              G ND FW+QF +E PG +   EV
Sbjct: 303 DSRLKSPPSPRIIDMNCEPDGSKEQNTVAAPPPPPVAGANDGFWQQFFSENPGSTEQREV 362

[Close]

BLAST of CmoCh02G000520 vs. ExPASy Swiss-Prot
Match: Q40152 (Heat shock factor protein HSF8 OS=Solanum lycopersicum OX=4081 GN=HSF8 PE=3 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 3.0e-48
Identity = 99/194 (51.03%), Postives = 132/194 (68.04%), Query Frame = 0


Query: 11  APPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKHNNFSSFVRQL 70
           APPPFL KTY+MVDDP T+ +VSWS +  SFVVW+PPEFAK+LLP YFKHNNFSSFVRQL
Sbjct: 36  APPPFLVKTYDMVDDPSTDKIVSWSPTNNSFVVWDPPEFAKDLLPKYFKHNNFSSFVRQL 95

Query: 71  NTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQSHG----------- 130
           NTYGFRKVD D+WEFANEGF+RG+ HLLKSI RRKP   H+Q Q Q HG           
Sbjct: 96  NTYGFRKVDPDRWEFANEGFLRGQKHLLKSISRRKPAHGHAQQQQQPHGNAQQQMQPPGH 155

Query: 131 --SGAPLSELERQELELKIKTLHQEKTILQTQLQEHENEKEQIGRQIQTMCQQIWRMGNQ 190
             S     E+ +  LE +++ L ++K +L  +L     +++    Q+Q M Q++  M  +
Sbjct: 156 SASVGACVEVGKFGLEEEVERLKRDKNVLMQELVRLRQQQQATDNQLQGMVQRLQGMELR 215

Query: 191 QKQLIAIMAAELQK 192
           Q+Q+++ +A  + +
Sbjct: 216 QQQMMSFLAKAVNR 229

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BLAST of CmoCh02G000520 vs. ExPASy TrEMBL
Match: A0A6J1G8J3 (heat stress transcription factor A-4d-like OS=Cucurbita moschata OX=3662 GN=LOC111451807 PE=3 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 4.0e-176
Identity = 313/313 (100.00%), Postives = 313/313 (100.00%), Query Frame = 0


Query: 1   MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH 60
           MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH
Sbjct: 1   MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQSHGS 120
           NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQSHGS
Sbjct: 61  NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQSHGS 120

Query: 121 GAPLSELERQELELKIKTLHQEKTILQTQLQEHENEKEQIGRQIQTMCQQIWRMGNQQKQ 180
           GAPLSELERQELELKIKTLHQEKTILQTQLQEHENEKEQIGRQIQTMCQQIWRMGNQQKQ
Sbjct: 121 GAPLSELERQELELKIKTLHQEKTILQTQLQEHENEKEQIGRQIQTMCQQIWRMGNQQKQ 180

Query: 181 LIAIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDERNGLKNGLEVPALELMGKLEVS 240
           LIAIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDERNGLKNGLEVPALELMGKLEVS
Sbjct: 181 LIAIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDERNGLKNGLEVPALELMGKLEVS 240

Query: 241 LGLCEDLLCNVAEVLGGEMSGKRKEMEGKGVKEGEKRGENGVNDVFWEQFLTEVPGCSNG 300
           LGLCEDLLCNVAEVLGGEMSGKRKEMEGKGVKEGEKRGENGVNDVFWEQFLTEVPGCSNG
Sbjct: 241 LGLCEDLLCNVAEVLGGEMSGKRKEMEGKGVKEGEKRGENGVNDVFWEQFLTEVPGCSNG 300

Query: 301 GEVYLDRRSNVLR 314
           GEVYLDRRSNVLR
Sbjct: 301 GEVYLDRRSNVLR 313

[Close]

BLAST of CmoCh02G000520 vs. ExPASy TrEMBL
Match: A0A6J1I3T7 (heat stress transcription factor A-4c-like OS=Cucurbita maxima OX=3661 GN=LOC111469660 PE=3 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 6.8e-168
Identity = 305/315 (96.83%), Postives = 307/315 (97.46%), Query Frame = 0


Query: 1   MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH 60
           MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH
Sbjct: 1   MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSH--SQSQSQSH 120
           NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSH  SQSQSQSH
Sbjct: 61  NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQSQSH 120

Query: 121 GSGAPLSELERQELELKIKTLHQEKTILQTQLQEHENEKEQIGRQIQTMCQQIWRMGNQQ 180
           GSGAPLSELERQELELKIKTLHQEKTILQTQLQEHE+EKEQIGRQIQTMCQQIWRMGNQQ
Sbjct: 121 GSGAPLSELERQELELKIKTLHQEKTILQTQLQEHEHEKEQIGRQIQTMCQQIWRMGNQQ 180

Query: 181 KQLIAIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDERNGLKNGLEVPALELMGKLE 240
           KQLIAIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDE+NGLK    VPALELMGKLE
Sbjct: 181 KQLIAIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDEKNGLK----VPALELMGKLE 240

Query: 241 VSLGLCEDLLCNVAEVLGGEMSGKRKEMEGKGVKEGEKRGENGVNDVFWEQFLTEVPGCS 300
           VSLGLCEDLLCNVAEVLGGEMS KRKEMEGKGVKEGEKRGENGVNDVFWEQFLTEVPGCS
Sbjct: 241 VSLGLCEDLLCNVAEVLGGEMSEKRKEMEGKGVKEGEKRGENGVNDVFWEQFLTEVPGCS 300

Query: 301 NGGEVYLDRRSNVLR 314
           N GEVYLDRRSNVLR
Sbjct: 301 NAGEVYLDRRSNVLR 311

[Close]

BLAST of CmoCh02G000520 vs. ExPASy TrEMBL
Match: A0A0A0KBI1 (HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G169310 PE=3 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 2.0e-119
Identity = 229/313 (73.16%), Postives = 268/313 (85.62%), Query Frame = 0


Query: 1   MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH 60
           M+GS+GS  GAPPPFLTKTYEMVDDPMTNS+VSW++SG+SFVVWNPPEFA+ELLP+YFKH
Sbjct: 1   MDGSEGSSTGAPPPFLTKTYEMVDDPMTNSIVSWNQSGFSFVVWNPPEFAQELLPIYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQSHGS 120
           NNFSSFVRQLNTYGFRK+DR+QWEFANEGFIRG+THLLKSIHRRKPI+SHSQS SQ +G 
Sbjct: 61  NNFSSFVRQLNTYGFRKIDREQWEFANEGFIRGKTHLLKSIHRRKPIYSHSQS-SQGNG- 120

Query: 121 GAPLSELERQELELKIKTLHQEKTILQTQLQEHENEKEQIGRQIQTMCQQIWRMGNQQKQ 180
           GAPLSE ER ELE KIKTL+QEKT LQ+QLQ+HENEKEQIG QIQ +C+++WRMGNQQKQ
Sbjct: 121 GAPLSEQERHELEQKIKTLYQEKTNLQSQLQKHENEKEQIGHQIQRICEKLWRMGNQQKQ 180

Query: 181 LIAIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDERNGLKNGLEVPALELMGKLEVS 240
           LI I+ AEL+K++ RK+RKIGK++EFL EE  E E+D  N  K  + VP LEL+GKLE+S
Sbjct: 181 LIGILGAELEKNEHRKKRKIGKVNEFLVEELTEFEKD--NLKKKKVNVPPLELLGKLELS 240

Query: 241 LGLCEDLLCNVAEVLGGEMSGKRKEMEGKGVKEGEKRGENGVNDVFWEQFLTEVPGCSNG 300
           LGLCEDLL NV +VL      + KEME K  KEGE R  +GVNDVFWE FLTE+PG SN 
Sbjct: 241 LGLCEDLLSNVGQVL-----KEGKEMEVK--KEGEMR--SGVNDVFWEHFLTEIPGSSNV 300

Query: 301 GEVYLDRRSNVLR 314
            +V+LDRR+NV+R
Sbjct: 301 TQVHLDRRNNVVR 300

[Close]

BLAST of CmoCh02G000520 vs. ExPASy TrEMBL
Match: A0A5D3BGW6 (Heat stress transcription factor A-4c-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold203G00240 PE=3 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.7e-118
Identity = 227/313 (72.52%), Postives = 265/313 (84.66%), Query Frame = 0


Query: 1   MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH 60
           M  S+GS  GAPPPFLTKTYEMVDDPM+NS+VSWS+SG+SFVVWNPPEFA+ELLP+YFKH
Sbjct: 1   MVSSEGSSSGAPPPFLTKTYEMVDDPMSNSIVSWSQSGFSFVVWNPPEFAQELLPIYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQSHGS 120
           NNFSSFVRQLNTYGFRK+DR+QWEFANEGFIRG+THLLKSIHRRKP++SHSQS SQ +G 
Sbjct: 61  NNFSSFVRQLNTYGFRKIDREQWEFANEGFIRGKTHLLKSIHRRKPMYSHSQS-SQGNG- 120

Query: 121 GAPLSELERQELELKIKTLHQEKTILQTQLQEHENEKEQIGRQIQTMCQQIWRMGNQQKQ 180
           GAPLSE ERQELE KIKTL+QEKT L++QLQ+HENEKEQIG QIQ +C+++WRMG+QQKQ
Sbjct: 121 GAPLSEQERQELEHKIKTLYQEKTNLESQLQKHENEKEQIGHQIQRICEKLWRMGHQQKQ 180

Query: 181 LIAIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDERNGLKNGLEVPALELMGKLEVS 240
           LI I+ AEL+K + RK+RK+GK++E L EEW E ERD+    K  + V  LELMGKLE+S
Sbjct: 181 LIGILGAELEKHEQRKKRKMGKVNELLVEEWTEFERDDLK--KKKVNVSPLELMGKLELS 240

Query: 241 LGLCEDLLCNVAEVLGGEMSGKRKEMEGKGVKEGEKRGENGVNDVFWEQFLTEVPGCSNG 300
           L LCEDLLCNVA+VL      + KEME K  KEGE R  +GVNDVFWE FLTE+PG S  
Sbjct: 241 LELCEDLLCNVAQVL-----KEGKEMEVK--KEGEMR--SGVNDVFWEHFLTEIPGSSKV 300

Query: 301 GEVYLDRRSNVLR 314
            EVYLDRR+NV+R
Sbjct: 301 TEVYLDRRNNVVR 300

[Close]

BLAST of CmoCh02G000520 vs. ExPASy TrEMBL
Match: A0A1S3C373 (heat stress transcription factor A-4c-like OS=Cucumis melo OX=3656 GN=LOC103496341 PE=3 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.7e-118
Identity = 227/313 (72.52%), Postives = 265/313 (84.66%), Query Frame = 0


Query: 1   MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH 60
           M  S+GS  GAPPPFLTKTYEMVDDPM+NS+VSWS+SG+SFVVWNPPEFA+ELLP+YFKH
Sbjct: 1   MVSSEGSSSGAPPPFLTKTYEMVDDPMSNSIVSWSQSGFSFVVWNPPEFAQELLPIYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQSHGS 120
           NNFSSFVRQLNTYGFRK+DR+QWEFANEGFIRG+THLLKSIHRRKP++SHSQS SQ +G 
Sbjct: 61  NNFSSFVRQLNTYGFRKIDREQWEFANEGFIRGKTHLLKSIHRRKPMYSHSQS-SQGNG- 120

Query: 121 GAPLSELERQELELKIKTLHQEKTILQTQLQEHENEKEQIGRQIQTMCQQIWRMGNQQKQ 180
           GAPLSE ERQELE KIKTL+QEKT L++QLQ+HENEKEQIG QIQ +C+++WRMG+QQKQ
Sbjct: 121 GAPLSEQERQELEHKIKTLYQEKTNLESQLQKHENEKEQIGHQIQRICEKLWRMGHQQKQ 180

Query: 181 LIAIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDERNGLKNGLEVPALELMGKLEVS 240
           LI I+ AEL+K + RK+RK+GK++E L EEW E ERD+    K  + V  LELMGKLE+S
Sbjct: 181 LIGILGAELEKHEQRKKRKMGKVNELLVEEWTEFERDDLK--KKKVNVSPLELMGKLELS 240

Query: 241 LGLCEDLLCNVAEVLGGEMSGKRKEMEGKGVKEGEKRGENGVNDVFWEQFLTEVPGCSNG 300
           L LCEDLLCNVA+VL      + KEME K  KEGE R  +GVNDVFWE FLTE+PG S  
Sbjct: 241 LELCEDLLCNVAQVL-----KEGKEMEVK--KEGEMR--SGVNDVFWEHFLTEIPGSSKV 300

Query: 301 GEVYLDRRSNVLR 314
            EVYLDRR+NV+R
Sbjct: 301 TEVYLDRRNNVVR 300

[Close]

BLAST of CmoCh02G000520 vs. NCBI nr
Match: XP_022948138.1 (heat stress transcription factor A-4d-like [Cucurbita moschata] >XP_022948139.1 heat stress transcription factor A-4d-like [Cucurbita moschata])

HSP 1 Score: 627.1 bits (1616), Expect = 8.2e-176
Identity = 313/313 (100.00%), Postives = 313/313 (100.00%), Query Frame = 0


Query: 1   MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH 60
           MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH
Sbjct: 1   MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQSHGS 120
           NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQSHGS
Sbjct: 61  NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQSHGS 120

Query: 121 GAPLSELERQELELKIKTLHQEKTILQTQLQEHENEKEQIGRQIQTMCQQIWRMGNQQKQ 180
           GAPLSELERQELELKIKTLHQEKTILQTQLQEHENEKEQIGRQIQTMCQQIWRMGNQQKQ
Sbjct: 121 GAPLSELERQELELKIKTLHQEKTILQTQLQEHENEKEQIGRQIQTMCQQIWRMGNQQKQ 180

Query: 181 LIAIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDERNGLKNGLEVPALELMGKLEVS 240
           LIAIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDERNGLKNGLEVPALELMGKLEVS
Sbjct: 181 LIAIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDERNGLKNGLEVPALELMGKLEVS 240

Query: 241 LGLCEDLLCNVAEVLGGEMSGKRKEMEGKGVKEGEKRGENGVNDVFWEQFLTEVPGCSNG 300
           LGLCEDLLCNVAEVLGGEMSGKRKEMEGKGVKEGEKRGENGVNDVFWEQFLTEVPGCSNG
Sbjct: 241 LGLCEDLLCNVAEVLGGEMSGKRKEMEGKGVKEGEKRGENGVNDVFWEQFLTEVPGCSNG 300

Query: 301 GEVYLDRRSNVLR 314
           GEVYLDRRSNVLR
Sbjct: 301 GEVYLDRRSNVLR 313

[Close]

BLAST of CmoCh02G000520 vs. NCBI nr
Match: XP_023534238.1 (heat stress transcription factor A-4c-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 618.6 bits (1594), Expect = 2.9e-173
Identity = 311/315 (98.73%), Postives = 313/315 (99.37%), Query Frame = 0


Query: 1   MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH 60
           MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH
Sbjct: 1   MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSH--SQSQSQSH 120
           NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSH  SQSQSQSH
Sbjct: 61  NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQSQSH 120

Query: 121 GSGAPLSELERQELELKIKTLHQEKTILQTQLQEHENEKEQIGRQIQTMCQQIWRMGNQQ 180
           GSGAPLSELERQELELKIKTLHQEKTILQTQLQEHE+EKEQIGRQIQTMCQQIWRMGNQQ
Sbjct: 121 GSGAPLSELERQELELKIKTLHQEKTILQTQLQEHEHEKEQIGRQIQTMCQQIWRMGNQQ 180

Query: 181 KQLIAIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDERNGLKNGLEVPALELMGKLE 240
           KQLIAIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDERNGLKNGL+VPALELMGKLE
Sbjct: 181 KQLIAIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDERNGLKNGLKVPALELMGKLE 240

Query: 241 VSLGLCEDLLCNVAEVLGGEMSGKRKEMEGKGVKEGEKRGENGVNDVFWEQFLTEVPGCS 300
           VSLGLCEDLLCNVAEVLGGEMSGKRKEMEGKGVKEGEKRGENGVNDVFWEQFLTEVPGCS
Sbjct: 241 VSLGLCEDLLCNVAEVLGGEMSGKRKEMEGKGVKEGEKRGENGVNDVFWEQFLTEVPGCS 300

Query: 301 NGGEVYLDRRSNVLR 314
           NGGEVYLDRRSNVLR
Sbjct: 301 NGGEVYLDRRSNVLR 315

[Close]

BLAST of CmoCh02G000520 vs. NCBI nr
Match: KAG6604741.1 (Heat stress transcription factor A-4b, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 616.3 bits (1588), Expect = 1.4e-172
Identity = 310/315 (98.41%), Postives = 312/315 (99.05%), Query Frame = 0


Query: 1   MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH 60
           MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH
Sbjct: 1   MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSH--SQSQSQSH 120
           NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSH  SQSQSQSH
Sbjct: 61  NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQSQSH 120

Query: 121 GSGAPLSELERQELELKIKTLHQEKTILQTQLQEHENEKEQIGRQIQTMCQQIWRMGNQQ 180
           GSGAPLSELERQELELKIKTLHQEKTILQTQLQEHE+EKEQIGRQIQTMCQQIWRMGNQQ
Sbjct: 121 GSGAPLSELERQELELKIKTLHQEKTILQTQLQEHEHEKEQIGRQIQTMCQQIWRMGNQQ 180

Query: 181 KQLIAIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDERNGLKNGLEVPALELMGKLE 240
           KQLIAIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDERNGLKNGL+VPALELMGKLE
Sbjct: 181 KQLIAIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDERNGLKNGLKVPALELMGKLE 240

Query: 241 VSLGLCEDLLCNVAEVLGGEMSGKRKEMEGKGVKEGEKRGENGVNDVFWEQFLTEVPGCS 300
           VSLGLCEDLLCNVAEVLGGEMSGKRKEMEGKGVKEGEKRGENGVNDVFWEQFLTEVPGCS
Sbjct: 241 VSLGLCEDLLCNVAEVLGGEMSGKRKEMEGKGVKEGEKRGENGVNDVFWEQFLTEVPGCS 300

Query: 301 NGGEVYLDRRSNVLR 314
           NGGEVYLDRRSNV R
Sbjct: 301 NGGEVYLDRRSNVSR 315

[Close]

BLAST of CmoCh02G000520 vs. NCBI nr
Match: KAG7034870.1 (Heat stress transcription factor A-4b, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 615.5 bits (1586), Expect = 2.5e-172
Identity = 310/317 (97.79%), Postives = 312/317 (98.42%), Query Frame = 0


Query: 1   MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH 60
           MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH
Sbjct: 1   MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSH----SQSQSQ 120
           NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSH    SQSQSQ
Sbjct: 61  NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQSQSQ 120

Query: 121 SHGSGAPLSELERQELELKIKTLHQEKTILQTQLQEHENEKEQIGRQIQTMCQQIWRMGN 180
           SHGSGAPLSELERQELELKIKTLHQEKTILQTQLQEHE+EKEQIGRQIQTMCQQIWRMGN
Sbjct: 121 SHGSGAPLSELERQELELKIKTLHQEKTILQTQLQEHEHEKEQIGRQIQTMCQQIWRMGN 180

Query: 181 QQKQLIAIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDERNGLKNGLEVPALELMGK 240
           QQKQLIAIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDERNGLKNGL+VPALELMGK
Sbjct: 181 QQKQLIAIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDERNGLKNGLKVPALELMGK 240

Query: 241 LEVSLGLCEDLLCNVAEVLGGEMSGKRKEMEGKGVKEGEKRGENGVNDVFWEQFLTEVPG 300
           LEVSLGLCEDLLCNVAEVLGGEMSGKRKEMEGKGVKEGEKRGENGVNDVFWEQFLTEVPG
Sbjct: 241 LEVSLGLCEDLLCNVAEVLGGEMSGKRKEMEGKGVKEGEKRGENGVNDVFWEQFLTEVPG 300

Query: 301 CSNGGEVYLDRRSNVLR 314
           CSNGGEVYLDRRSNV R
Sbjct: 301 CSNGGEVYLDRRSNVSR 317

[Close]

BLAST of CmoCh02G000520 vs. NCBI nr
Match: XP_022970770.1 (heat stress transcription factor A-4c-like [Cucurbita maxima])

HSP 1 Score: 599.7 bits (1545), Expect = 1.4e-167
Identity = 305/315 (96.83%), Postives = 307/315 (97.46%), Query Frame = 0


Query: 1   MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH 60
           MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH
Sbjct: 1   MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSH--SQSQSQSH 120
           NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSH  SQSQSQSH
Sbjct: 61  NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQSQSH 120

Query: 121 GSGAPLSELERQELELKIKTLHQEKTILQTQLQEHENEKEQIGRQIQTMCQQIWRMGNQQ 180
           GSGAPLSELERQELELKIKTLHQEKTILQTQLQEHE+EKEQIGRQIQTMCQQIWRMGNQQ
Sbjct: 121 GSGAPLSELERQELELKIKTLHQEKTILQTQLQEHEHEKEQIGRQIQTMCQQIWRMGNQQ 180

Query: 181 KQLIAIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDERNGLKNGLEVPALELMGKLE 240
           KQLIAIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDE+NGLK    VPALELMGKLE
Sbjct: 181 KQLIAIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDEKNGLK----VPALELMGKLE 240

Query: 241 VSLGLCEDLLCNVAEVLGGEMSGKRKEMEGKGVKEGEKRGENGVNDVFWEQFLTEVPGCS 300
           VSLGLCEDLLCNVAEVLGGEMS KRKEMEGKGVKEGEKRGENGVNDVFWEQFLTEVPGCS
Sbjct: 241 VSLGLCEDLLCNVAEVLGGEMSEKRKEMEGKGVKEGEKRGENGVNDVFWEQFLTEVPGCS 300

Query: 301 NGGEVYLDRRSNVLR 314
           N GEVYLDRRSNVLR
Sbjct: 301 NAGEVYLDRRSNVLR 311

[Close]

BLAST of CmoCh02G000520 vs. TAIR 10
Match: AT5G45710.1 (winged-helix DNA-binding transcription factor family protein )

HSP 1 Score: 202.6 bits (514), Expect = 4.7e-52
Identity = 131/328 (39.94%), Postives = 187/328 (57.01%), Query Frame = 0


Query: 5   DGSYGGAP--PPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKHNN 64
           D + GG+   PPFLTKTYEMVDD  ++SVV+WS++  SF+V NP EF+++LLP +FKH N
Sbjct: 2   DENNGGSSSLPPFLTKTYEMVDDSSSDSVVAWSENNKSFIVKNPAEFSRDLLPRFFKHKN 61

Query: 65  FSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQSHGSGA 124
           FSSF+RQLNTYGFRKVD ++WEF N+ F+RGR +L+K+IHRRKP+ SHS    Q+     
Sbjct: 62  FSSFIRQLNTYGFRKVDPEKWEFLNDDFVRGRPYLMKNIHRRKPVHSHSLVNLQAQN--- 121

Query: 125 PLSELERQELELKIKTLHQEKTILQTQLQEHENEKEQIGRQIQTMCQQIWRMGNQQKQLI 184
           PL+E ER+ +E +I+ L  EK  L  +LQ  E E+++   Q+ T+  ++  M   QK ++
Sbjct: 122 PLTESERRSMEDQIERLKNEKEGLLAELQNQEQERKEFELQVTTLKDRLQHMEQHQKSIV 181

Query: 185 AIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDERNGLKNGLEVPA--LELMGKLEVS 244
           A ++           + +GK    L  E    ER +R   +N L   +  +E + KLE S
Sbjct: 182 AYVS-----------QVLGKPGLSLNLE--NHERRKRRFQENSLPPSSSHIEQVEKLESS 241

Query: 245 LGLCEDLLCNVAEVLGGEMSGKRKEMEGKGVKEGEKRGEN-------------------- 304
           L   E+L+    E  G + S    +     +  G+ R ++                    
Sbjct: 242 LTFWENLVSESCEKSGLQSSSMDHDAAESSLSIGDTRPKSSKIDMNSEPPVTVTAPAPKT 301

Query: 305 GVNDVFWEQFLTEVPGCSNGGEVYLDRR 309
           GVND FWEQ LTE PG +   EV  +RR
Sbjct: 302 GVNDDFWEQCLTENPGSTEQQEVQSERR 313

[Close]

BLAST of CmoCh02G000520 vs. TAIR 10
Match: AT4G18880.1 (heat shock transcription factor A4A )

HSP 1 Score: 196.1 bits (497), Expect = 4.4e-50
Identity = 123/367 (33.51%), Postives = 195/367 (53.13%), Query Frame = 0


Query: 2   EGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKHN 61
           E + G    + PPFLTKTYEMVDD  ++S+VSWS+S  SF+VWNPPEF+++LLP +FKHN
Sbjct: 3   ENNHGVSSSSLPPFLTKTYEMVDDSSSDSIVSWSQSNKSFIVWNPPEFSRDLLPRFFKHN 62

Query: 62  NFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQSHGSG 121
           NFSSF+RQLNTYGFRK D +QWEFAN+ F+RG+ HL+K+IHRRKP+ SHS    Q+  + 
Sbjct: 63  NFSSFIRQLNTYGFRKADPEQWEFANDDFVRGQPHLMKNIHRRKPVHSHSLPNLQAQLN- 122

Query: 122 APLSELERQELELKIKTLHQEKTILQTQLQEHENEKEQIGRQIQTMCQQIWRMGNQQKQL 181
            PL++ ER  +  +I+ L +EK  L  +L + + E+E    Q++ + +++  M  +QK +
Sbjct: 123 -PLTDSERVRMNNQIERLTKEKEGLLEELHKQDEEREVFEMQVKELKERLQHMEKRQKTM 182

Query: 182 IAIMAAELQK------------DQSRKRRKIGKLSEFLGEEWLEVERDERNGLKNGLEVP 241
           ++ ++  L+K            + + ++R+  ++  F  E  LE  +      + G   P
Sbjct: 183 VSFVSQVLEKPGLALNLSPCVPETNERKRRFPRIEFFPDEPMLEENKTCVVVREEGSTSP 242

Query: 242 ALEL----MGKLEVSLGLCEDLLCNVAEVLGGEMSGKRKEMEGKGV-------------- 301
           +       + +LE S+ + E+L+ +  E +    S    +++                  
Sbjct: 243 SSHTREHQVEQLESSIAIWENLVSDSCESMLQSRSMMTLDVDESSTFPESPPLSCIQLSV 302

Query: 302 ------------------KEGEKRGEN----------GVNDVFWEQFLTEVPGCSNGGEV 311
                              +G K              G ND FW+QF +E PG +   EV
Sbjct: 303 DSRLKSPPSPRIIDMNCEPDGSKEQNTVAAPPPPPVAGANDGFWQQFFSENPGSTEQREV 362

[Close]

BLAST of CmoCh02G000520 vs. TAIR 10
Match: AT1G32330.1 (heat shock transcription factor A1D )

HSP 1 Score: 188.3 bits (477), Expect = 9.1e-48
Identity = 103/215 (47.91%), Postives = 143/215 (66.51%), Query Frame = 0


Query: 11  APPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKHNNFSSFVRQL 70
           APPPFL+KTY+MVDD  T+S+VSWS +  SF+VW PPEFA++LLP  FKHNNFSSFVRQL
Sbjct: 34  APPPFLSKTYDMVDDHNTDSIVSWSANNNSFIVWKPPEFARDLLPKNFKHNNFSSFVRQL 93

Query: 71  NTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQS-HGSGAPLS---- 130
           NTYGFRKVD D+WEFANEGF+RG+ HLL+SI RRKP     Q   +S H +G   S    
Sbjct: 94  NTYGFRKVDPDRWEFANEGFLRGQKHLLQSITRRKPAHGQGQGHQRSQHSNGQNSSVSAC 153

Query: 131 -ELERQELELKIKTLHQEKTILQTQLQEHENEKEQIGRQIQTMCQQIWRMGNQQKQLIAI 190
            E+ +  LE +++ L ++K +L  +L     +++    Q+QTM Q++  M N+Q+QL++ 
Sbjct: 154 VEVGKFGLEEEVERLKRDKNVLMQELVRLRQQQQSTDNQLQTMVQRLQGMENRQQQLMSF 213

Query: 191 MAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDER 220
           +A  +Q            LS+FL ++  + E + R
Sbjct: 214 LAKAVQSPHF--------LSQFLQQQNQQNESNRR 240

[Close]

BLAST of CmoCh02G000520 vs. TAIR 10
Match: AT4G17750.1 (heat shock factor 1 )

HSP 1 Score: 183.0 bits (463), Expect = 3.8e-46
Identity = 93/190 (48.95%), Postives = 134/190 (70.53%), Query Frame = 0


Query: 12  PPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKHNNFSSFVRQLN 71
           PPPFL+KTY+MV+DP T+++VSWS +  SF+VW+PPEF+++LLP YFKHNNFSSFVRQLN
Sbjct: 50  PPPFLSKTYDMVEDPATDAIVSWSPTNNSFIVWDPPEFSRDLLPKYFKHNNFSSFVRQLN 109

Query: 72  TYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSH--------SQSQSQSHGSGAP 131
           TYGFRKVD D+WEFANEGF+RG+ HLLK I RRK +  H        SQ  SQ  GS A 
Sbjct: 110 TYGFRKVDPDRWEFANEGFLRGQKHLLKKISRRKSVQGHGSSSSNPQSQQLSQGQGSMAA 169

Query: 132 LS---ELERQELELKIKTLHQEKTILQTQLQEHENEKEQIGRQIQTMCQQIWRMGNQQKQ 191
           LS   E+ +  LE +++ L ++K +L  +L +   +++    ++Q + + +  M  +Q+Q
Sbjct: 170 LSSCVEVGKFGLEEEVEQLKRDKNVLMQELVKLRQQQQTTDNKLQVLVKHLQVMEQRQQQ 229

[Close]

BLAST of CmoCh02G000520 vs. TAIR 10
Match: AT5G16820.1 (heat shock factor 3 )

HSP 1 Score: 180.3 bits (456), Expect = 2.5e-45
Identity = 95/181 (52.49%), Postives = 129/181 (71.27%), Query Frame = 0


Query: 13  PPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKHNNFSSFVRQLNT 72
           PPFL+KTY+MVDDP+TN VVSWS    SFVVW+ PEF+K LLP YFKHNNFSSFVRQLNT
Sbjct: 26  PPFLSKTYDMVDDPLTNEVVSWSSGNNSFVVWSAPEFSKVLLPKYFKHNNFSSFVRQLNT 85

Query: 73  YGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIF---SHSQSQSQSHGSGAPLSELER 132
           YGFRKVD D+WEFANEGF+RGR  LLKSI RRKP     +  Q+Q QS   GA + E+ +
Sbjct: 86  YGFRKVDPDRWEFANEGFLRGRKQLLKSIVRRKPSHVQQNQQQTQVQSSSVGACV-EVGK 145

Query: 133 QELELKIKTLHQEKTILQTQLQEHENEKEQIGRQIQTMCQQIWRMGNQQKQLIAIMAAEL 191
             +E +++ L ++K +L  +L     +++    Q+Q + Q++  M  +Q+Q+++ +A  +
Sbjct: 146 FGIEEEVERLKRDKNVLMQELVRLRQQQQATENQLQNVGQKVQVMEQRQQQMMSFLAKAV 205

The following BLAST results are available for this feature:

BLAST of CmoCh02G000520 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93VB5	2.0e-52	45.32	Heat stress transcription factor A-4d OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
Q9FK72	6.5e-51	39.94	Heat stress transcription factor A-4c OS=Arabidopsis thaliana OX=3702 GN=HSFA4C ...	[more]
Q94J16	1.6e-49	44.12	Heat stress transcription factor A-4b OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
O49403	6.1e-49	33.51	Heat stress transcription factor A-4a OS=Arabidopsis thaliana OX=3702 GN=HSFA4A ...	[more]
Q40152	3.0e-48	51.03	Heat shock factor protein HSF8 OS=Solanum lycopersicum OX=4081 GN=HSF8 PE=3 SV=1	[more]

BLAST of CmoCh02G000520 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G8J3	4.0e-176	100.00	heat stress transcription factor A-4d-like OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1I3T7	6.8e-168	96.83	heat stress transcription factor A-4c-like OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A0A0KBI1	2.0e-119	73.16	HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G169310 ...	[more]
A0A5D3BGW6	1.7e-118	72.52	Heat stress transcription factor A-4c-like OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A1S3C373	1.7e-118	72.52	heat stress transcription factor A-4c-like OS=Cucumis melo OX=3656 GN=LOC1034963...	[more]

BLAST of CmoCh02G000520 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022948138.1	8.2e-176	100.00	heat stress transcription factor A-4d-like [Cucurbita moschata] >XP_022948139.1 ...	[more]
XP_023534238.1	2.9e-173	98.73	heat stress transcription factor A-4c-like [Cucurbita pepo subsp. pepo]	[more]
KAG6604741.1	1.4e-172	98.41	Heat stress transcription factor A-4b, partial [Cucurbita argyrosperma subsp. so...	[more]
KAG7034870.1	2.5e-172	97.79	Heat stress transcription factor A-4b, partial [Cucurbita argyrosperma subsp. ar...	[more]
XP_022970770.1	1.4e-167	96.83	heat stress transcription factor A-4c-like [Cucurbita maxima]	[more]

BLAST of CmoCh02G000520 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G45710.1	4.7e-52	39.94	winged-helix DNA-binding transcription factor family protein	[more]
AT4G18880.1	4.4e-50	33.51	heat shock transcription factor A4A	[more]
AT1G32330.1	9.1e-48	47.91	heat shock transcription factor A1D	[more]
AT4G17750.1	3.8e-46	48.95	heat shock factor 1	[more]
AT5G16820.1	2.5e-45	52.49	heat shock factor 3	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 129..170
None	No IPR available	PANTHER	PTHR10015:SF312	HEAT STRESS TRANSCRIPTION FACTOR A-4D	coord: 9..198
IPR000232	Heat shock factor (HSF)-type, DNA-binding	PRINTS	PR00056	HSFDOMAIN	coord: 53..65 score: 78.24 coord: 66..78 score: 87.03 coord: 15..38 score: 48.45
IPR000232	Heat shock factor (HSF)-type, DNA-binding	SMART	SM00415	hsfneu3	coord: 11..104 e-value: 3.0E-61 score: 219.5
IPR000232	Heat shock factor (HSF)-type, DNA-binding	PFAM	PF00447	HSF_DNA-bind	coord: 15..104 e-value: 5.1E-32 score: 110.4
IPR000232	Heat shock factor (HSF)-type, DNA-binding	PROSITE	PS00434	HSF_DOMAIN	coord: 54..78
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 9..105 e-value: 3.0E-38 score: 132.4
IPR027725	Heat shock transcription factor family	PANTHER	PTHR10015	HEAT SHOCK TRANSCRIPTION FACTOR	coord: 9..198
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 11..104

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh02G000520.1	CmoCh02G000520.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0034605	cellular response to heat
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0000978	RNA polymerase II cis-regulatory region sequence-specific DNA binding
molecular_function	GO:0043565	sequence-specific DNA binding