CmoCh01G020610 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh01G020610
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	4-coumarate--CoA ligase
Location	Cmo_Chr01: 14389013 .. 14392370 (-)
RNA-Seq Expression	CmoCh01G020610
Synteny	CmoCh01G020610

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AACATATTAGTATTATTATTATTGTTTACAGAAAAAGTTTGGCTCGTCAACGACGGCGGCTCCTCAGCTTCTATACAAGCCAACTCCGACTCAATCCCCCACTGCCCAAAATCAATCTCAAAGCTCAGTTACAAAATTCCCTTTTCTCCGGCAACCAGATGGCGGAACACGGCGGCGAAATAGATCCAAGAAGCGGGTTCTGCAAATCCACCAAAATCTTCCACAGCAAGCGCCACCCAATCCCTCTCCCCCCAAACCAATCCCTCGATGCGACGACCTTCATCTCTTCCCGCCCTCACAACGGCAAAATCGCCCTCATCGACGCCACCACCGGCCAACACATAACCTATTCCCACCTCTGGGAATCCGTCGATTCCGTCGCCTCTGCTCTCTCCGACATGGGAATCCGAAAAGGCCACGTCATCCTCCTCCTCTCCCCTAACTCCATTTTCTTCCCCATCATTTGCCTCGCCGTCATGTCCATCGGCGCCATCATCACCACCACAAATCCCCTCAATACTCCCCAAGAAATCGCCAAGCAGATCGCCGATTCAAAACCCATTTTAGCTTTCACAACCCAACAATTACTCCCCAAAATCGCCAGCTCCAAATTGCCAATCGTCCTTATTGACGGCGAAATTCAAACCCCCTCAGTCAAAATCGTGTCCACGTTGAACGAAATGATGAGGAAAAAACCCAGTGAGAGTAGAATCAAAGAACGGGTTGAGCAAAACGACACAGCGACTCTGCTTTATTCGTCTGGAACGACAGGGGCGAGCAAGGGCGTAGTGTCGTCTCATAAAAATTTAATTGCGATGGTCCAAGTGGTCGTGACGAGGTTCAGATTAAGCGAAGGGGAAGGGACTTTTATCTGTACGGTTCCGATGTTTCATATCTATGGACTCGTGGCCTTCGCGACGGGGCTGCTGTCTTCAGGATCCACGATCATCGTGTTGTCCAAATTCGAAATTCATGAGATGTTGTCTGCGATTGATAAGTACAGGGCAACGTATCTGCCACTTGTGCCCCCGATTCTAGTGGCGCTAGTGAACGCCGCCGAGCAGATAAAGGGCAAGTACGATTTGAGCTCCTTGCACACGGCGTTGTCCGGCGGAGCGCCGCTGGGGAAGGAGGTCATTGAGGGTTTTGTTGAGAAATATCCGAATGTTGCGATTCTTCAGGGGTATGGGTTGACGGAGTCCACCGGAATAGGAGCTTCCACTGATTCTCTGGAGGAGAGCCGCCGGTATGGCACGGCGGGGCTTTTGTCTCCGAGCACTGAGGCAATGATTGTTGACCCGGAAACTGGCAAGCCTCTGCCCGTGAACCGGACCGGAGAGCTTTGGCTCAGAGGACCCACCGTTATGAAAGGTAAGAATTTGGTCAATGGCGACATGTGTCGAGTCAAAGAATATAAGCTTTAATTAATTTCCTTAATTTTAAGAATACTCCTTAAGACAATGATTTTATCTTATATAAATATTTTTTTAGAATATCGTGTTTCTTTTCAAAAAAATAATTCAGGATCATATGGTATTTTTTTAAATTAATCTTTTATTTTAACAATAGTGCTATGCATCCTCAAATATATATAATTTATAAATTTAATTCGCAAACCATATATTTTATTTTTCTTATTAAATATCAACAATTTTATAGTATTTAATTATTTAACTACAAAATTTCCCAATTTATTCCTTCAGTTTGATAAAATAAATTTTACTAAACAGTTTTATTTATTATTATTATTATTTTTACATTGGTTGTTGTAGGGTATTTTGGTAATGTGGAGGCCACAGCCTCAACGCTCGACTCAAAGGGATGGTTGAGAACGGGCGATCTGTGTTACATCGATCAAGATGGTTTTATTTTTGTGGTTGATAGGCTCAAAGAACTCATCAAATACAAGGGTTATCAGGTAATTTTACATGGATTTTCTTTCAAAAAAATCACTACATTTTTAAAAGTAAAACCACCCCATAAAAAATTATTATTTTTAGATAATTTATATATTAATTTAACATAATTTGAGTTTATATGACATTTTTGTCTTAAATTAATCTCTTTTTTTTTTTCATTTATATGCTATTTTGTAAAATAAGTTAATTTATATTGATTTGACATTTTTTAGAAAAATGGAGGGTAAAAAGGTCAATATACATGTCTCTCACCATGAGAATCATGGGAATCTCAAGAAACTCATGCGCATCATAACTTTATGGATATCTCTAGATAAACGATCAATGAACGAAAAATTATTGAAAATGTGTTTATTTGCATCACTTTTTTAATGATTAATTTTACTGATTTTTTGTTTATTTAATATTGACGTCCAGGTGCCTCCAGCCGAATTAGAGGCGTTATTACTCACACATCCTAATATCTCTGATGCAGCTGTGATCCCGTGAGTTCTCAAAATTCATATTTTTAATATGATATTTAAAATTGAAAAAAGAAACAAATAATTTTAGATCCAAATACACTTTTTATATTGATCACGTGATCAATATAAAAATATCTCAAACCATTAGAATACGTAAAAGCCTCCTTTAATTATTAAATAAAATAATTATCCTTCAAAAGGCAGTGGGCCAGCAGGCTGCGATATTTTATTATGCCATCCATGGTTTTCATTCGCTGTTTAAAAAGAAGCAAGGAGTGGACAAAATTACACTTCTATCCCTAGATTGTATTTTTATTTACTACTCACCATGTCTTAATCAACAAAACAACACAATTAGGCTTTGAATTTAATATTTTTGTTAAATATTTCATTGTAAACGATTTATTCCAAGGATTACTAGTGTCTTTATTATACTTTATTATATTAAAAGGTTTCGCAATATTGTCTATTTAATTTGAAGTATATGATTTTTAATAAGTAATTTTTTTTTCCTTTAATAATTCCAGTTTTCCAGACAAGGAGGTTGGGCAGTATCCAATGGCCTACGTGGTAGGAAAGGGTGGAAGTGACATTTCACATGAAGATGTAATGCAATTCGCGGCAAAACAGGTCAGTATTAATATCAGTCGGTGTAAAATTTGGAACATAGTAAATAAATTTCATACTATTTTTAATAATATTTGGTCTAAATTAACAAAATTTATAATCTAATTAATTAAGTTTGTTTAATTAGTAGAAATAAAATTTCTATTATTTAAAAAATGGTTAATTATTATTATTATTTTATCTGGTCAACTCTATAATCATAAGCTGAGAAGGGGTCCACGTGTGCTTGTACAGGTGGCGCCGTACAAGAGAATCCGGCGGGTGGCTTTTGTGGATTCCATTCCCAAAAATCCGTCCGGTAAGATTTTGAGAAAGGATCTCATAAAGCTTGCAACCTCTAGACTCTGA

mRNA sequence

AACATATTAGTATTATTATTATTGTTTACAGAAAAAGTTTGGCTCGTCAACGACGGCGGCTCCTCAGCTTCTATACAAGCCAACTCCGACTCAATCCCCCACTGCCCAAAATCAATCTCAAAGCTCAGTTACAAAATTCCCTTTTCTCCGGCAACCAGATGGCGGAACACGGCGGCGAAATAGATCCAAGAAGCGGGTTCTGCAAATCCACCAAAATCTTCCACAGCAAGCGCCACCCAATCCCTCTCCCCCCAAACCAATCCCTCGATGCGACGACCTTCATCTCTTCCCGCCCTCACAACGGCAAAATCGCCCTCATCGACGCCACCACCGGCCAACACATAACCTATTCCCACCTCTGGGAATCCGTCGATTCCGTCGCCTCTGCTCTCTCCGACATGGGAATCCGAAAAGGCCACGTCATCCTCCTCCTCTCCCCTAACTCCATTTTCTTCCCCATCATTTGCCTCGCCGTCATGTCCATCGGCGCCATCATCACCACCACAAATCCCCTCAATACTCCCCAAGAAATCGCCAAGCAGATCGCCGATTCAAAACCCATTTTAGCTTTCACAACCCAACAATTACTCCCCAAAATCGCCAGCTCCAAATTGCCAATCGTCCTTATTGACGGCGAAATTCAAACCCCCTCAGTCAAAATCGTGTCCACGTTGAACGAAATGATGAGGAAAAAACCCAGTGAGAGTAGAATCAAAGAACGGGTTGAGCAAAACGACACAGCGACTCTGCTTTATTCGTCTGGAACGACAGGGGCGAGCAAGGGCGTAGTGTCGTCTCATAAAAATTTAATTGCGATGGTCCAAGTGGTCGTGACGAGGTTCAGATTAAGCGAAGGGGAAGGGACTTTTATCTGTACGGTTCCGATGTTTCATATCTATGGACTCGTGGCCTTCGCGACGGGGCTGCTGTCTTCAGGATCCACGATCATCGTGTTGTCCAAATTCGAAATTCATGAGATGTTGTCTGCGATTGATAAGTACAGGGCAACGTATCTGCCACTTGTGCCCCCGATTCTAGTGGCGCTAGTGAACGCCGCCGAGCAGATAAAGGGCAAGTACGATTTGAGCTCCTTGCACACGGCGTTGTCCGGCGGAGCGCCGCTGGGGAAGGAGGTCATTGAGGGTTTTGTTGAGAAATATCCGAATGTTGCGATTCTTCAGGGGTATGGGTTGACGGAGTCCACCGGAATAGGAGCTTCCACTGATTCTCTGGAGGAGAGCCGCCGGTATGGCACGGCGGGGCTTTTGTCTCCGAGCACTGAGGCAATGATTGTTGACCCGGAAACTGGCAAGCCTCTGCCCGTGAACCGGACCGGAGAGCTTTGGCTCAGAGGACCCACCGTTATGAAAGAATATCGTGTTTCTTTTCAAAAAAATAATTCAGGATCATATGGGTATTTTGGTAATGTGGAGGCCACAGCCTCAACGCTCGACTCAAAGGGATGGTTGAGAACGGGCGATCTGTGTTACATCGATCAAGATGGTTTTATTTTTGTGGTTGATAGGCTCAAAGAACTCATCAAATACAAGGGTTATCAGGTGCCTCCAGCCGAATTAGAGGCGTTATTACTCACACATCCTAATATCTCTGATGCAGCTGTGATCCCTTTTCCAGACAAGGAGGTTGGGCAGTATCCAATGGCCTACGTGGTAGGAAAGGGTGGAAGTGACATTTCACATGAAGATGTAATGCAATTCGCGGCAAAACAGGTCAAAATAAAATTTCTATTATTTAAAAAATGGTTAATTATTATTATTATTTTATCTGGTCAACTCTATAATCATAAGCTGAGAAGGGGTCCACGTGTGCTTGTACAGGTGGCGCCGTACAAGAGAATCCGGCGGGTGGCTTTTGTGGATTCCATTCCCAAAAATCCGTCCGGTAAGATTTTGAGAAAGGATCTCATAAAGCTTGCAACCTCTAGACTCTGA

Coding sequence (CDS)

ATGGCGGAACACGGCGGCGAAATAGATCCAAGAAGCGGGTTCTGCAAATCCACCAAAATCTTCCACAGCAAGCGCCACCCAATCCCTCTCCCCCCAAACCAATCCCTCGATGCGACGACCTTCATCTCTTCCCGCCCTCACAACGGCAAAATCGCCCTCATCGACGCCACCACCGGCCAACACATAACCTATTCCCACCTCTGGGAATCCGTCGATTCCGTCGCCTCTGCTCTCTCCGACATGGGAATCCGAAAAGGCCACGTCATCCTCCTCCTCTCCCCTAACTCCATTTTCTTCCCCATCATTTGCCTCGCCGTCATGTCCATCGGCGCCATCATCACCACCACAAATCCCCTCAATACTCCCCAAGAAATCGCCAAGCAGATCGCCGATTCAAAACCCATTTTAGCTTTCACAACCCAACAATTACTCCCCAAAATCGCCAGCTCCAAATTGCCAATCGTCCTTATTGACGGCGAAATTCAAACCCCCTCAGTCAAAATCGTGTCCACGTTGAACGAAATGATGAGGAAAAAACCCAGTGAGAGTAGAATCAAAGAACGGGTTGAGCAAAACGACACAGCGACTCTGCTTTATTCGTCTGGAACGACAGGGGCGAGCAAGGGCGTAGTGTCGTCTCATAAAAATTTAATTGCGATGGTCCAAGTGGTCGTGACGAGGTTCAGATTAAGCGAAGGGGAAGGGACTTTTATCTGTACGGTTCCGATGTTTCATATCTATGGACTCGTGGCCTTCGCGACGGGGCTGCTGTCTTCAGGATCCACGATCATCGTGTTGTCCAAATTCGAAATTCATGAGATGTTGTCTGCGATTGATAAGTACAGGGCAACGTATCTGCCACTTGTGCCCCCGATTCTAGTGGCGCTAGTGAACGCCGCCGAGCAGATAAAGGGCAAGTACGATTTGAGCTCCTTGCACACGGCGTTGTCCGGCGGAGCGCCGCTGGGGAAGGAGGTCATTGAGGGTTTTGTTGAGAAATATCCGAATGTTGCGATTCTTCAGGGGTATGGGTTGACGGAGTCCACCGGAATAGGAGCTTCCACTGATTCTCTGGAGGAGAGCCGCCGGTATGGCACGGCGGGGCTTTTGTCTCCGAGCACTGAGGCAATGATTGTTGACCCGGAAACTGGCAAGCCTCTGCCCGTGAACCGGACCGGAGAGCTTTGGCTCAGAGGACCCACCGTTATGAAAGAATATCGTGTTTCTTTTCAAAAAAATAATTCAGGATCATATGGGTATTTTGGTAATGTGGAGGCCACAGCCTCAACGCTCGACTCAAAGGGATGGTTGAGAACGGGCGATCTGTGTTACATCGATCAAGATGGTTTTATTTTTGTGGTTGATAGGCTCAAAGAACTCATCAAATACAAGGGTTATCAGGTGCCTCCAGCCGAATTAGAGGCGTTATTACTCACACATCCTAATATCTCTGATGCAGCTGTGATCCCTTTTCCAGACAAGGAGGTTGGGCAGTATCCAATGGCCTACGTGGTAGGAAAGGGTGGAAGTGACATTTCACATGAAGATGTAATGCAATTCGCGGCAAAACAGGTCAAAATAAAATTTCTATTATTTAAAAAATGGTTAATTATTATTATTATTTTATCTGGTCAACTCTATAATCATAAGCTGAGAAGGGGTCCACGTGTGCTTGTACAGGTGGCGCCGTACAAGAGAATCCGGCGGGTGGCTTTTGTGGATTCCATTCCCAAAAATCCGTCCGGTAAGATTTTGAGAAAGGATCTCATAAAGCTTGCAACCTCTAGACTCTGA

Protein sequence

MAEHGGEIDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDATTGQHITYSHLWESVDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLNTPQEIAKQIADSKPILAFTTQQLLPKIASSKLPIVLIDGEIQTPSVKIVSTLNEMMRKKPSESRIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEGTFICTVPMFHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIDKYRATYLPLVPPILVALVNAAEQIKGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTDSLEESRRYGTAGLLSPSTEAMIVDPETGKPLPVNRTGELWLRGPTVMKEYRVSFQKNNSGSYGYFGNVEATASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNISDAAVIPFPDKEVGQYPMAYVVGKGGSDISHEDVMQFAAKQVKIKFLLFKKWLIIIIILSGQLYNHKLRRGPRVLVQVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATSRL

Homology

[Close]

BLAST of CmoCh01G020610 vs. ExPASy Swiss-Prot
Match: M4IQR7 (Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1)

HSP 1 Score: 805.1 bits (2078), Expect = 5.5e-232
Identity = 418/593 (70.49%), Postives = 473/593 (79.76%), Query Frame = 0


Query: 8   IDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDATTGQHITYSHL 67
           +D RSG+CKS  IF+SKR P+ LP N S+D TTFISSR H+GKIA IDA TG+H+T+  L
Sbjct: 9   VDERSGYCKSNSIFYSKREPVQLPQNHSVDVTTFISSRAHHGKIAFIDAATGRHLTFPQL 68

Query: 68  WESVDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLNTPQEIAK 127
           W +VDSVA+ LS MGIRKG VILLLSPNSI+FP++CLAVMS+GAIITTTNPLNTP+EIAK
Sbjct: 69  WRAVDSVATCLSAMGIRKGDVILLLSPNSIYFPVVCLAVMSLGAIITTTNPLNTPREIAK 128

Query: 128 QIADSKPILAFTTQQLLPKIASSKLPIVLIDGEIQT---PSVKIVSTLNEMMRKKPSESR 187
           QI DSKP+LAFT  QL+ KIA S LPIV+ID E+++    ++ IVS+L EMMRK+PS +R
Sbjct: 129 QITDSKPVLAFTIPQLVSKIAGSNLPIVIIDDEVKSSLEKTLNIVSSLGEMMRKEPSPNR 188

Query: 188 IKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEGTFICTVPMF 247
           I  RV Q DTATLLYSSGTTGASKGVVSSHKNLIAMVQ +++RF   +GE TFICTVPMF
Sbjct: 189 IGYRVNQEDTATLLYSSGTTGASKGVVSSHKNLIAMVQTILSRFGTEDGEHTFICTVPMF 248

Query: 248 HIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIDKYRATYLPLVPPILVALVNAAEQIK 307
           HIYGL AFA GLLSSGSTI++LSKFEIHEMLSAI+KYRATYLPLVPPIL+AL+  A  I+
Sbjct: 249 HIYGLAAFAMGLLSSGSTIVILSKFEIHEMLSAIEKYRATYLPLVPPILMALLKNANHIR 308

Query: 308 GKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTDSLEESRRY 367
            KYDLSSL + LSGGAPL KEVIEGFVE YP V+ILQGYGLTESTGIGASTD L+ESRRY
Sbjct: 309 AKYDLSSLQSVLSGGAPLSKEVIEGFVENYPTVSILQGYGLTESTGIGASTDCLQESRRY 368

Query: 368 GTAGLLSPSTEAMIVDPETGKPLPVNRTGELWLRGPTVMKEYRVSFQKNNSGSYGYFGNV 427
           GTAG+LSPS EA IV+PETG+ L VNRTGELWLRGPT+MK              GYF N 
Sbjct: 369 GTAGMLSPSMEAKIVNPETGEALSVNRTGELWLRGPTIMK--------------GYFSNE 428

Query: 428 EATASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNIS 487
           EAT+ST+DS+GWLRTGDLCYID+DGFIFVVDRLKELIKYKGYQV PAELEALLL+HP IS
Sbjct: 429 EATSSTIDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLSHPEIS 488

Query: 488 DAAVIPFPDKEVGQYPMAYVVGKGGSDISHEDVMQFAAKQVKIKFLLFKKWLIIIIILSG 547
           DAAVIP+PDKE GQ+PMAYVV KGGS++S   VM F AK                     
Sbjct: 489 DAAVIPYPDKEAGQFPMAYVVRKGGSNLSESTVMDFIAK--------------------- 548

Query: 548 QLYNHKLRRGPRVLVQVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATSRL 598
                           VAPYKRIR+VAFV SIPKNPSGKILRKDLIKLATS+L
Sbjct: 549 ---------------LVAPYKRIRKVAFVASIPKNPSGKILRKDLIKLATSKL 551

[Close]

BLAST of CmoCh01G020610 vs. ExPASy Swiss-Prot
Match: Q84P21 (4-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana OX=3702 GN=4CLL5 PE=1 SV=2)

HSP 1 Score: 778.1 bits (2008), Expect = 7.2e-224
Identity = 406/590 (68.81%), Postives = 464/590 (78.64%), Query Frame = 0


Query: 8   IDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDATTGQHITYSHL 67
           ++ RSGFC S   F+SKR PIPLPPN SLD TTFISS+ H G+IA IDA+TGQ++T++ L
Sbjct: 4   VNSRSGFCNSNSTFYSKRTPIPLPPNPSLDVTTFISSQAHRGRIAFIDASTGQNLTFTEL 63

Query: 68  WESVDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLNTPQEIAK 127
           W +V+SVA  LS++GIRKGHV+LLLSPNSI FP++CL+VMS+GAIITTTNPLNT  EIAK
Sbjct: 64  WRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNEIAK 123

Query: 128 QIADSKPILAFTTQQLLPKI--ASSKLPIVLIDGEIQTPSVKIVSTLNEMMRKKPSESRI 187
           QI DS P+LAFTT QLLPKI  A+ KLPIVL+D E +  SV  V  L EMM+K+PS +R+
Sbjct: 124 QIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEE-RVDSVGDVRRLVEMMKKEPSGNRV 183

Query: 188 KERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEGTFICTVPMFH 247
           KERV+Q+DTATLLYSSGTTG SKGV+SSH+NLIAMVQ +V RF   +GE  FICTVPMFH
Sbjct: 184 KERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQRFICTVPMFH 243

Query: 248 IYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIDKYRATYLPLVPPILVALVNAAEQIKG 307
           IYGL AFATGLL+ GSTIIVLSKFE+HEM+SAI KY+AT LPLVPPILVA+VN A+QIK 
Sbjct: 244 IYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNGADQIKA 303

Query: 308 KYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTDSLEESRRYG 367
           KYDLSS+HT L GGAPL KEV EGF EKYP V ILQGYGLTESTGIGASTD++EESRRYG
Sbjct: 304 KYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVEESRRYG 363

Query: 368 TAGLLSPSTEAMIVDPETGKPLPVNRTGELWLRGPTVMKEYRVSFQKNNSGSYGYFGNVE 427
           TAG LS S E  IVDP TG+ L   +TGELWL+GP++MK              GYF N E
Sbjct: 364 TAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMK--------------GYFSNEE 423

Query: 428 ATASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNISD 487
           AT+STLDS+GWLRTGDLCYID+DGFIFVVDRLKELIKYKGYQV PAELEALLLTHP I+D
Sbjct: 424 ATSSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITD 483

Query: 488 AAVIPFPDKEVGQYPMAYVVGKGGSDISHEDVMQFAAKQVKIKFLLFKKWLIIIIILSGQ 547
           AAVIPFPDKEVGQ+PMAYVV K GS +S + +M+F AK                      
Sbjct: 484 AAVIPFPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAK---------------------- 542

Query: 548 LYNHKLRRGPRVLVQVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATS 596
                         QVAPYKRIR+VAFV SIPKNPSGKILRKDLIK+ATS
Sbjct: 544 --------------QVAPYKRIRKVAFVSSIPKNPSGKILRKDLIKIATS 542

[Close]

BLAST of CmoCh01G020610 vs. ExPASy Swiss-Prot
Match: Q10S72 (4-coumarate--CoA ligase-like 4 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL4 PE=2 SV=1)

HSP 1 Score: 679.1 bits (1751), Expect = 4.6e-194
Identity = 353/601 (58.74%), Postives = 444/601 (73.88%), Query Frame = 0


Query: 7   EIDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDATTGQHITYSH 66
           E+D RSG+C +T+ F S+R  +PLP +  +D  +F++SR H+G +AL+DA TG+ IT++ 
Sbjct: 8   EVDARSGYCAATRTFRSRRADVPLPADPEVDVVSFLASRRHSGVVALVDAATGRRITFTE 67

Query: 67  LWESVDSVASALS--DMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLNTPQE 126
           LW +V   ASAL+   + +RKGHV L+LSPNS+ FP+  LA MS+GA++TT NPLNTP E
Sbjct: 68  LWRAVAGAASALAAHPVSLRKGHVALILSPNSVHFPVAALAAMSLGAVLTTANPLNTPAE 127

Query: 127 IAKQIADSKPILAFTTQQLLPKI-ASSKLPIVLID-----GEIQTPSVKIVSTLNEMMRK 186
           IAKQ+AD++P+LAFTT++LLPK+  +  L +VL++     G+   P  +IV+T+ E+   
Sbjct: 128 IAKQVADARPVLAFTTRELLPKLPRAHDLRVVLLESARLPGDSSDP--RIVATIEEISAT 187

Query: 187 KPSESRIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRL--SEGEGT 246
            P  +R K+RV Q+D ATLLYSSGTTG SKGVV++H++LI+MVQ+++TRFRL  S+   T
Sbjct: 188 TPDPARRKDRVTQDDPATLLYSSGTTGPSKGVVATHRSLISMVQIIMTRFRLEGSDKTET 247

Query: 247 FICTVPMFHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIDKYRATYLPLVPPILVAL 306
           F+CTVPMFH+YGLVAFATGLL  G+T++VLSK+E+ EML +I+ Y  TYLPLVPPILVA+
Sbjct: 248 FLCTVPMFHVYGLVAFATGLLGCGATVVVLSKYELPEMLRSINAYGVTYLPLVPPILVAM 307

Query: 307 VNAAEQIKGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTD 366
           V   + +     L  +   LSGGAPLGKE+IEGF EKYP V ILQGYGLTEST IGASTD
Sbjct: 308 VAHPKPL----PLGQMRKVLSGGAPLGKELIEGFREKYPQVEILQGYGLTESTAIGASTD 367

Query: 367 SLEESRRYGTAGLLSPSTEAMIVDPETGKPLPVNRTGELWLRGPTVMKEYRVSFQKNNSG 426
           S EESRRYGTAGLLSP+TEA IVDP++G+ LPVNRTGELW+RGP VMK            
Sbjct: 368 SAEESRRYGTAGLLSPNTEAKIVDPDSGEALPVNRTGELWIRGPYVMK------------ 427

Query: 427 SYGYFGNVEATASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEAL 486
             GYF N EAT STL   GWL+TGDLCYID+DG++FVVDRLKELIKYKGYQVPPAELEAL
Sbjct: 428 --GYFKNAEATQSTLTPDGWLKTGDLCYIDEDGYLFVVDRLKELIKYKGYQVPPAELEAL 487

Query: 487 LLTHPNISDAAVIPFPDKEVGQYPMAYVVGKGGSDISHEDVMQFAAKQVKIKFLLFKKWL 546
           LLTHP ++D AVIPFPD+EVGQ+PMAY+V K GS++S  +VM+F AK             
Sbjct: 488 LLTHPEVTDVAVIPFPDREVGQFPMAYIVRKKGSNLSEREVMEFVAK------------- 547

Query: 547 IIIIILSGQLYNHKLRRGPRVLVQVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATSR 598
                                  QVAPYK++R+VAFV  IPKN SGKILRKDLIKLATS+
Sbjct: 548 -----------------------QVAPYKKVRKVAFVTDIPKNASGKILRKDLIKLATSK 552

[Close]

BLAST of CmoCh01G020610 vs. ExPASy Swiss-Prot
Match: P0C5B6 (OPC-6:CoA ligase OS=Arabidopsis thaliana OX=3702 GN=4CLL4 PE=2 SV=1)

HSP 1 Score: 659.4 bits (1700), Expect = 3.7e-188
Identity = 340/594 (57.24%), Postives = 430/594 (72.39%), Query Frame = 0


Query: 8   IDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDATTGQHITYSHL 67
           +DPRSGFCKS   F+SKR P+ LPPN S D TTFISS+PH GK A IDA TGQ +T+S L
Sbjct: 10  VDPRSGFCKSNSTFYSKRQPLSLPPNLSRDVTTFISSQPHRGKTAFIDAATGQCLTFSDL 69

Query: 68  WESVDSVASAL-SDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLNTPQEIA 127
           W +VD VA  L  ++GIR+G V+L+LSPNSIF P++CL+VMS+GA+ TT N LNT  EI+
Sbjct: 70  WRAVDRVADCLYHEVGIRRGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTLNTSGEIS 129

Query: 128 KQIADSKPILAFTTQQLLPKIASSKLPIVLIDGEIQ---TPSVKIVSTLNEMMRKKPSES 187
           KQIADS P L FTT+QL PK+  + + +VL D E+    T ++++V  L+EM++K+PS  
Sbjct: 130 KQIADSNPTLVFTTRQLAPKLPVA-ISVVLTDDEVYQELTSAIRVVGILSEMVKKEPSGQ 189

Query: 188 RIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEGTFICTVPM 247
           R+++RV Q+DTA +LYSSGTTG SKGV+SSH+NL A V   ++     + +  FICTVPM
Sbjct: 190 RVRDRVNQDDTAMMLYSSGTTGPSKGVISSHRNLTAHVARFIS--DNLKRDDIFICTVPM 249

Query: 248 FHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIDKYRATYLPLVPPILVALVNAAEQI 307
           FH YGL+ FA G ++ GST+++L +F++H+M+ A++K+RAT L L PP+LVA++N A+ I
Sbjct: 250 FHTYGLLTFAMGTVALGSTVVILRRFQLHDMMDAVEKHRATALALAPPVLVAMINDADLI 309

Query: 308 KGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTDSLEESRR 367
           K KYDLSSL T   GGAPL KEV EGF+EKYP V ILQGY LTES G GA T+S EESRR
Sbjct: 310 KAKYDLSSLKTVRCGGAPLSKEVTEGFLEKYPTVDILQGYALTESNGGGAFTNSAEESRR 369

Query: 368 YGTAGLLSPSTEAMIVDPETGKPLPVNRTGELWLRGPTVMKEYRVSFQKNNSGSYGYFGN 427
           YGTAG L+   EA IVDP TG+ + +N+TGELWL+GP++ K              GYF N
Sbjct: 370 YGTAGTLTSDVEARIVDPNTGRFMGINQTGELWLKGPSISK--------------GYFKN 429

Query: 428 VEATASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNI 487
            EAT  T++ +GWL+TGDLCYID+DGF+FVVDRLKELIKYKGYQVPPAELEALL+THP+I
Sbjct: 430 QEATNETINLEGWLKTGDLCYIDEDGFLFVVDRLKELIKYKGYQVPPAELEALLITHPDI 489

Query: 488 SDAAVIPFPDKEVGQYPMAYVVGKGGSDISHEDVMQFAAKQVKIKFLLFKKWLIIIIILS 547
            DAAVIPFPDKE GQYPMAYVV K  S++S + V+ F +K                    
Sbjct: 490 LDAAVIPFPDKEAGQYPMAYVVRKHESNLSEKQVIDFISK-------------------- 549

Query: 548 GQLYNHKLRRGPRVLVQVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATSRL 598
                           QVAPYK+IR+V+F++SIPK  SGK LRKDLIKLATS+L
Sbjct: 550 ----------------QVAPYKKIRKVSFINSIPKTASGKTLRKDLIKLATSKL 550

[Close]

BLAST of CmoCh01G020610 vs. ExPASy Swiss-Prot
Match: Q84P25 (4-coumarate--CoA ligase-like 2 OS=Arabidopsis thaliana OX=3702 GN=4CLL2 PE=2 SV=2)

HSP 1 Score: 651.7 bits (1680), Expect = 7.8e-186
Identity = 339/599 (56.59%), Postives = 431/599 (71.95%), Query Frame = 0


Query: 8   IDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDATTGQHITYSHL 67
           +D +SGFC+ST IF+SKR P+ LPPNQ LD T+FI+S+PH GK   +DA TG+ +++  L
Sbjct: 21  VDRKSGFCESTSIFYSKREPMALPPNQFLDVTSFIASQPHRGKTVFVDAVTGRRLSFPEL 80

Query: 68  WESVDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLNTPQEIAK 127
           W  V+ VA  L  +G+RKG+V+++LSPNSI FPI+ L+VMS+GAIITT NP+NT  EI+K
Sbjct: 81  WLGVERVAGCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPINTSDEISK 140

Query: 128 QIADSKPILAFTTQQLLPKIASS---KLPIVLIDGEIQTPS------VKIVSTLNEMMRK 187
           QI DS+P+LAFTT +L+ K+A++    LP+VL+D +   PS      VK+V  L  M+  
Sbjct: 141 QIGDSRPVLAFTTCKLVSKLAAASNFNLPVVLMD-DYHVPSQSYGDRVKLVGRLETMIET 200

Query: 188 KPSESRIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEGTFI 247
           +PSESR+K+RV Q+DTA LLYSSGTTG SKGV+ SH+NLIA+VQ    RF L   E   I
Sbjct: 201 EPSESRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIALVQAYRARFGL---EQRTI 260

Query: 248 CTVPMFHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIDKYRATYLPLVPPILVALVN 307
           CT+PM HI+G   FATGL++ G TI+VL KF++ ++LSA++ +R++YL LVPPI+VA+VN
Sbjct: 261 CTIPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYLSLVPPIVVAMVN 320

Query: 308 AAEQIKGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTDSL 367
            A +I  KYDLSSLHT ++GGAPL +EV E FVE YP V ILQGYGLTEST I AS  + 
Sbjct: 321 GANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYGLTESTAIAASMFNK 380

Query: 368 EESRRYGTAGLLSPSTEAMIVDPETGKPLPVNRTGELWLRGPTVMKEYRVSFQKNNSGSY 427
           EE++RYG +GLL+P+ E  IVDP+TG+ L VN+TGELW+R PTVMK              
Sbjct: 381 EETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMK-------------- 440

Query: 428 GYFGNVEATASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEALLL 487
           GYF N EATAST+DS+GWL+TGDLCYID DGF+FVVDRLKELIK  GYQV PAELEALLL
Sbjct: 441 GYFKNKEATASTIDSEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLL 500

Query: 488 THPNISDAAVIPFPDKEVGQYPMAYVVGKGGSDISHEDVMQFAAKQVKIKFLLFKKWLII 547
            HP I+DAAVIP PD + GQYPMAY+V K GS++S  ++M F AK               
Sbjct: 501 AHPEIADAAVIPIPDMKAGQYPMAYIVRKVGSNLSESEIMGFVAK--------------- 560

Query: 548 IIILSGQLYNHKLRRGPRVLVQVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATSRL 598
                                QV+PYK+IR+V F+ SIPKNPSGKILR++L KL TS+L
Sbjct: 561 ---------------------QVSPYKKIRKVTFLASIPKNPSGKILRRELTKLTTSKL 565

[Close]

BLAST of CmoCh01G020610 vs. ExPASy TrEMBL
Match: A0A6J1FMD2 (4-coumarate--CoA ligase-like 5 OS=Cucurbita moschata OX=3662 GN=LOC111446530 PE=4 SV=1)

HSP 1 Score: 1045.4 bits (2702), Expect = 8.9e-302
Identity = 547/597 (91.62%), Postives = 547/597 (91.62%), Query Frame = 0


Query: 1   MAEHGGEIDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDATTGQ 60
           MAEHGGEIDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDATTGQ
Sbjct: 1   MAEHGGEIDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDATTGQ 60

Query: 61  HITYSHLWESVDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLN 120
           HITYSHLWESVDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLN
Sbjct: 61  HITYSHLWESVDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLN 120

Query: 121 TPQEIAKQIADSKPILAFTTQQLLPKIASSKLPIVLIDGEIQTPSVKIVSTLNEMMRKKP 180
           TPQEIAKQIADSKPILAFTTQQLLPKIASSKLPIVLIDGEIQTPSVKIVSTLNEMMRKKP
Sbjct: 121 TPQEIAKQIADSKPILAFTTQQLLPKIASSKLPIVLIDGEIQTPSVKIVSTLNEMMRKKP 180

Query: 181 SESRIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEGTFICT 240
           SESRIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEGTFICT
Sbjct: 181 SESRIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEGTFICT 240

Query: 241 VPMFHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIDKYRATYLPLVPPILVALVNAA 300
           VPMFHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIDKYRATYLPLVPPILVALVNAA
Sbjct: 241 VPMFHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIDKYRATYLPLVPPILVALVNAA 300

Query: 301 EQIKGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTDSLEE 360
           EQIKGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTDSLEE
Sbjct: 301 EQIKGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTDSLEE 360

Query: 361 SRRYGTAGLLSPSTEAMIVDPETGKPLPVNRTGELWLRGPTVMKEYRVSFQKNNSGSYGY 420
           SRRYGTAGLLSPSTEAMIVDPETGKPLPVNRTGELWLRGPTVMK              GY
Sbjct: 361 SRRYGTAGLLSPSTEAMIVDPETGKPLPVNRTGELWLRGPTVMK--------------GY 420

Query: 421 FGNVEATASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEALLLTH 480
           FGNVEATASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEALLLTH
Sbjct: 421 FGNVEATASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEALLLTH 480

Query: 481 PNISDAAVIPFPDKEVGQYPMAYVVGKGGSDISHEDVMQFAAKQVKIKFLLFKKWLIIII 540
           PNISDAAVIPFPDKEVGQYPMAYVVGKGGSDISHEDVMQFAAK                 
Sbjct: 481 PNISDAAVIPFPDKEVGQYPMAYVVGKGGSDISHEDVMQFAAK----------------- 540

Query: 541 ILSGQLYNHKLRRGPRVLVQVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATSRL 598
                              QVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATSRL
Sbjct: 541 -------------------QVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATSRL 547

[Close]

BLAST of CmoCh01G020610 vs. ExPASy TrEMBL
Match: A0A6J1J2B8 (4-coumarate--CoA ligase-like 5 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481187 PE=4 SV=1)

HSP 1 Score: 1024.6 bits (2648), Expect = 1.6e-295
Identity = 536/597 (89.78%), Postives = 541/597 (90.62%), Query Frame = 0


Query: 1   MAEHGGEIDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDATTGQ 60
           MAEHGGEIDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDATTGQ
Sbjct: 1   MAEHGGEIDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDATTGQ 60

Query: 61  HITYSHLWESVDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLN 120
           HITYSHLWES+DSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLN
Sbjct: 61  HITYSHLWESLDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLN 120

Query: 121 TPQEIAKQIADSKPILAFTTQQLLPKIASSKLPIVLIDGEIQTPSVKIVSTLNEMMRKKP 180
           TPQEIAKQIADSKPILAFTTQQLLPKIASS LPIVLIDG+IQTPSVKIVSTLNEMMRKKP
Sbjct: 121 TPQEIAKQIADSKPILAFTTQQLLPKIASSTLPIVLIDGQIQTPSVKIVSTLNEMMRKKP 180

Query: 181 SESRIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEGTFICT 240
           SESRIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEGTFICT
Sbjct: 181 SESRIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEGTFICT 240

Query: 241 VPMFHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIDKYRATYLPLVPPILVALVNAA 300
           VPMFHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAI+KYRATYLPLVPPILVALVNAA
Sbjct: 241 VPMFHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIEKYRATYLPLVPPILVALVNAA 300

Query: 301 EQIKGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTDSLEE 360
           EQIKGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTDSLEE
Sbjct: 301 EQIKGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTDSLEE 360

Query: 361 SRRYGTAGLLSPSTEAMIVDPETGKPLPVNRTGELWLRGPTVMKEYRVSFQKNNSGSYGY 420
           SRRYGTAGLLSPSTEAMIVDPETG+ LPVNRTGELWLRGPTVMK              GY
Sbjct: 361 SRRYGTAGLLSPSTEAMIVDPETGEALPVNRTGELWLRGPTVMK--------------GY 420

Query: 421 FGNVEATASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEALLLTH 480
           FGNVEATASTLDS GWLRTGDLCYID+DGFIFVVDRLKELIKYKGYQVPPAELEALLL H
Sbjct: 421 FGNVEATASTLDSMGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLAH 480

Query: 481 PNISDAAVIPFPDKEVGQYPMAYVVGKGGSDISHEDVMQFAAKQVKIKFLLFKKWLIIII 540
           PNISDAAVIPFPDKEVGQYPMAYVV KGGSDISHEDVMQF AK                 
Sbjct: 481 PNISDAAVIPFPDKEVGQYPMAYVVRKGGSDISHEDVMQFVAK----------------- 540

Query: 541 ILSGQLYNHKLRRGPRVLVQVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATSRL 598
                              QVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATSRL
Sbjct: 541 -------------------QVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATSRL 547

[Close]

BLAST of CmoCh01G020610 vs. ExPASy TrEMBL
Match: A0A6J1F7P0 (4-coumarate--CoA ligase-like 5 OS=Cucurbita moschata OX=3662 GN=LOC111443102 PE=4 SV=1)

HSP 1 Score: 976.1 bits (2522), Expect = 6.6e-281
Identity = 508/602 (84.39%), Postives = 533/602 (88.54%), Query Frame = 0


Query: 1   MAEHGG---EIDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDAT 60
           MA +GG   E+DPRSGFCKSTKIFHSKR PIPLPPNQSLDATTFISSRPHNGKIALIDAT
Sbjct: 1   MAVYGGDVPEVDPRSGFCKSTKIFHSKRRPIPLPPNQSLDATTFISSRPHNGKIALIDAT 60

Query: 61  TGQHITYSHLWESVDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTN 120
           TGQHITYSHLWESVDSVAS+LSDMGIRKGHVILLLSPNSIFFPIICLAVMS+GA+ITTTN
Sbjct: 61  TGQHITYSHLWESVDSVASSLSDMGIRKGHVILLLSPNSIFFPIICLAVMSLGAVITTTN 120

Query: 121 PLNTPQEIAKQIADSKPILAFTTQQLLPKIASSKLPIVLIDGEIQTP--SVKIVSTLNEM 180
           PLNTPQEIAKQIADS PILAFTTQQL+PKIASSKLP+VLIDGEIQ    SVKIVSTL EM
Sbjct: 121 PLNTPQEIAKQIADSNPILAFTTQQLIPKIASSKLPVVLIDGEIQIKKFSVKIVSTLGEM 180

Query: 181 MRKKPSESRIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEG 240
           MRKK S SR+KERV+QNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRF+LSEGEG
Sbjct: 181 MRKKSSGSRVKERVDQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFKLSEGEG 240

Query: 241 TFICTVPMFHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIDKYRATYLPLVPPILVA 300
           TFICTVPMFHIYGLVAFATGLLSSGSTI+VLSKFEIHEMLSAI+KYRATYLPLVPPILVA
Sbjct: 241 TFICTVPMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIEKYRATYLPLVPPILVA 300

Query: 301 LVNAAEQIKGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGAST 360
           +VNAAEQIKGKYDL SLHTALSGGAPLGKEVIEGFVEKYPNV+ILQGYGLTESTGIGAST
Sbjct: 301 MVNAAEQIKGKYDLGSLHTALSGGAPLGKEVIEGFVEKYPNVSILQGYGLTESTGIGAST 360

Query: 361 DSLEESRRYGTAGLLSPSTEAMIVDPETGKPLPVNRTGELWLRGPTVMKEYRVSFQKNNS 420
           DSLEESRRYGTAGLLSPSTEA IVDP+TG+ LPVNRTGELWLRGPT+MK           
Sbjct: 361 DSLEESRRYGTAGLLSPSTEAKIVDPDTGEALPVNRTGELWLRGPTIMK----------- 420

Query: 421 GSYGYFGNVEATASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEA 480
              GYFGNVEAT STLDS+GWLRTGDLCY+D+DGFIFVVDRLKELIKYKGYQVPPAELEA
Sbjct: 421 ---GYFGNVEATTSTLDSEGWLRTGDLCYVDEDGFIFVVDRLKELIKYKGYQVPPAELEA 480

Query: 481 LLLTHPNISDAAVIPFPDKEVGQYPMAYVVGKGGSDISHEDVMQFAAKQVKIKFLLFKKW 540
           LLLTHP+ISDAAVIP+PDKEVGQYPMAYVV KGGSDISHED+MQF A+            
Sbjct: 481 LLLTHPSISDAAVIPYPDKEVGQYPMAYVVRKGGSDISHEDIMQFVAR------------ 540

Query: 541 LIIIIILSGQLYNHKLRRGPRVLVQVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATS 598
                                   QVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATS
Sbjct: 541 ------------------------QVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATS 552

[Close]

BLAST of CmoCh01G020610 vs. ExPASy TrEMBL
Match: A0A6J1II10 (4-coumarate--CoA ligase-like 5 OS=Cucurbita maxima OX=3661 GN=LOC111476465 PE=4 SV=1)

HSP 1 Score: 967.6 bits (2500), Expect = 2.4e-278
Identity = 505/602 (83.89%), Postives = 531/602 (88.21%), Query Frame = 0


Query: 1   MAEHGG---EIDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDAT 60
           MA +GG   ++DPRSGFCKSTKIFHSKR PIPLPPNQSLDATTFISSRPHNGKIALIDAT
Sbjct: 1   MAVYGGDVPQVDPRSGFCKSTKIFHSKRRPIPLPPNQSLDATTFISSRPHNGKIALIDAT 60

Query: 61  TGQHITYSHLWESVDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTN 120
           TGQHITYSHLWESVDSVAS+LSDMGIRKGHVILLLSPNSIFFPIICLAVMS+GA+ITTTN
Sbjct: 61  TGQHITYSHLWESVDSVASSLSDMGIRKGHVILLLSPNSIFFPIICLAVMSLGAVITTTN 120

Query: 121 PLNTPQEIAKQIADSKPILAFTTQQLLPKIASSKLPIVLIDGEIQTP--SVKIVSTLNEM 180
           PLNTPQEIAKQIADS PILAFTTQQL+PKIASSKLP+VLIDGEIQ    SVKIVSTL+EM
Sbjct: 121 PLNTPQEIAKQIADSNPILAFTTQQLIPKIASSKLPVVLIDGEIQIKKFSVKIVSTLSEM 180

Query: 181 MRKKPSESRIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEG 240
           MRKK S SR+KERV+QNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRF+LSEGEG
Sbjct: 181 MRKKSSGSRVKERVDQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFKLSEGEG 240

Query: 241 TFICTVPMFHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIDKYRATYLPLVPPILVA 300
           TFICTVPMFHIYGLVAFATGLLSSGSTI+VLSKFEIHEMLSAI+KYRATYLPLVPPILVA
Sbjct: 241 TFICTVPMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIEKYRATYLPLVPPILVA 300

Query: 301 LVNAAEQIKGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGAST 360
           +VNAAEQIKGKYDL SLHTALSGGAPLGKEVIEGFVEKYPNV+ILQGYGLTESTGIGAST
Sbjct: 301 MVNAAEQIKGKYDLGSLHTALSGGAPLGKEVIEGFVEKYPNVSILQGYGLTESTGIGAST 360

Query: 361 DSLEESRRYGTAGLLSPSTEAMIVDPETGKPLPVNRTGELWLRGPTVMKEYRVSFQKNNS 420
           DSLEESRRYGTAGLLSPSTEA IV P+TG+ LPVN TGELWLRGPT+MK           
Sbjct: 361 DSLEESRRYGTAGLLSPSTEAKIVVPDTGEALPVNTTGELWLRGPTIMK----------- 420

Query: 421 GSYGYFGNVEATASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEA 480
              GYFGNVEAT STLDS+GWLRTGDLCYID+DGFIFVVDRLKELIKYKGYQVPPAELEA
Sbjct: 421 ---GYFGNVEATTSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEA 480

Query: 481 LLLTHPNISDAAVIPFPDKEVGQYPMAYVVGKGGSDISHEDVMQFAAKQVKIKFLLFKKW 540
           LLLTHP+ISDAAVIP+PDKEVGQYPMAYVV KGGSDISHED+MQ  A+            
Sbjct: 481 LLLTHPSISDAAVIPYPDKEVGQYPMAYVVRKGGSDISHEDIMQLVAR------------ 540

Query: 541 LIIIIILSGQLYNHKLRRGPRVLVQVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATS 598
                                   QVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATS
Sbjct: 541 ------------------------QVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATS 552

[Close]

BLAST of CmoCh01G020610 vs. ExPASy TrEMBL
Match: A0A0A0L385 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G653450 PE=4 SV=1)

HSP 1 Score: 955.7 bits (2469), Expect = 9.3e-275
Identity = 499/600 (83.17%), Postives = 526/600 (87.67%), Query Frame = 0


Query: 1   MAEHGG---EIDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDAT 60
           MAE+GG   E+DPRSGFCKSTKIF+SKR PIPLPPNQSLDATTFISSRPHNGKIALIDA+
Sbjct: 1   MAEYGGENLEVDPRSGFCKSTKIFNSKRRPIPLPPNQSLDATTFISSRPHNGKIALIDAS 60

Query: 61  TGQHITYSHLWESVDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTN 120
           TG HITYS LW +V SVAS+LSDMGIRKGHVILLLSPNSI FPIICLAVMSIGAIITTTN
Sbjct: 61  TGHHITYSDLWNAVHSVASSLSDMGIRKGHVILLLSPNSIHFPIICLAVMSIGAIITTTN 120

Query: 121 PLNTPQEIAKQIADSKPILAFTTQQLLPKIASSKLPIVLIDGEIQTPSVKIVSTLNEMMR 180
           PLNTPQEI+KQI+DSKPILAFTTQ L+PKIA+SKLP+V+IDG+I     KIVSTL+EMMR
Sbjct: 121 PLNTPQEISKQISDSKPILAFTTQALIPKIATSKLPVVVIDGQIPKSQAKIVSTLSEMMR 180

Query: 181 KKPSESRIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEGTF 240
           KKPS S+IKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRF+LSEGEGTF
Sbjct: 181 KKPSGSQIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFKLSEGEGTF 240

Query: 241 ICTVPMFHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIDKYRATYLPLVPPILVALV 300
           ICTVPMFHIYGLVAFATGLLSSGSTI+VLSKFEIHEMLSAI+KY+ATYLPLVPPILVALV
Sbjct: 241 ICTVPMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIEKYKATYLPLVPPILVALV 300

Query: 301 NAAEQIKGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTDS 360
           NAAEQIKGKYDL SLHTALSGGAPLGKEVIEGFVEK+PNVAILQGYGLTESTGIGASTDS
Sbjct: 301 NAAEQIKGKYDLGSLHTALSGGAPLGKEVIEGFVEKFPNVAILQGYGLTESTGIGASTDS 360

Query: 361 LEESRRYGTAGLLSPSTEAMIVDPETGKPLPVNRTGELWLRGPTVMKEYRVSFQKNNSGS 420
           LEESRRYGTAGLLSPSTE MIVDPETG+ LPVNRTGELWLRGPTVMK             
Sbjct: 361 LEESRRYGTAGLLSPSTEGMIVDPETGEALPVNRTGELWLRGPTVMK------------- 420

Query: 421 YGYFGNVEATASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEALL 480
            GYFGNVEAT+STLDS GWLRTGDLCYID+DGFIFVVDRLKELIKYKGYQVPPAELEALL
Sbjct: 421 -GYFGNVEATSSTLDSAGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALL 480

Query: 481 LTHPNISDAAVIPFPDKEVGQYPMAYVVGKGGSDISHEDVMQFAAKQVKIKFLLFKKWLI 540
           LTHPNISDAAVIP+PDK+VGQ+PMAYVV K GSDISH DVMQF AK              
Sbjct: 481 LTHPNISDAAVIPYPDKDVGQFPMAYVVRKVGSDISHNDVMQFVAK-------------- 540

Query: 541 IIIILSGQLYNHKLRRGPRVLVQVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATSRL 598
                                 QVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATS+L
Sbjct: 541 ----------------------QVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATSKL 550

[Close]

BLAST of CmoCh01G020610 vs. NCBI nr
Match: XP_022941144.1 (4-coumarate--CoA ligase-like 5 [Cucurbita moschata])

HSP 1 Score: 1045.4 bits (2702), Expect = 1.8e-301
Identity = 547/597 (91.62%), Postives = 547/597 (91.62%), Query Frame = 0


Query: 1   MAEHGGEIDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDATTGQ 60
           MAEHGGEIDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDATTGQ
Sbjct: 1   MAEHGGEIDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDATTGQ 60

Query: 61  HITYSHLWESVDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLN 120
           HITYSHLWESVDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLN
Sbjct: 61  HITYSHLWESVDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLN 120

Query: 121 TPQEIAKQIADSKPILAFTTQQLLPKIASSKLPIVLIDGEIQTPSVKIVSTLNEMMRKKP 180
           TPQEIAKQIADSKPILAFTTQQLLPKIASSKLPIVLIDGEIQTPSVKIVSTLNEMMRKKP
Sbjct: 121 TPQEIAKQIADSKPILAFTTQQLLPKIASSKLPIVLIDGEIQTPSVKIVSTLNEMMRKKP 180

Query: 181 SESRIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEGTFICT 240
           SESRIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEGTFICT
Sbjct: 181 SESRIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEGTFICT 240

Query: 241 VPMFHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIDKYRATYLPLVPPILVALVNAA 300
           VPMFHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIDKYRATYLPLVPPILVALVNAA
Sbjct: 241 VPMFHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIDKYRATYLPLVPPILVALVNAA 300

Query: 301 EQIKGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTDSLEE 360
           EQIKGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTDSLEE
Sbjct: 301 EQIKGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTDSLEE 360

Query: 361 SRRYGTAGLLSPSTEAMIVDPETGKPLPVNRTGELWLRGPTVMKEYRVSFQKNNSGSYGY 420
           SRRYGTAGLLSPSTEAMIVDPETGKPLPVNRTGELWLRGPTVMK              GY
Sbjct: 361 SRRYGTAGLLSPSTEAMIVDPETGKPLPVNRTGELWLRGPTVMK--------------GY 420

Query: 421 FGNVEATASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEALLLTH 480
           FGNVEATASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEALLLTH
Sbjct: 421 FGNVEATASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEALLLTH 480

Query: 481 PNISDAAVIPFPDKEVGQYPMAYVVGKGGSDISHEDVMQFAAKQVKIKFLLFKKWLIIII 540
           PNISDAAVIPFPDKEVGQYPMAYVVGKGGSDISHEDVMQFAAK                 
Sbjct: 481 PNISDAAVIPFPDKEVGQYPMAYVVGKGGSDISHEDVMQFAAK----------------- 540

Query: 541 ILSGQLYNHKLRRGPRVLVQVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATSRL 598
                              QVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATSRL
Sbjct: 541 -------------------QVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATSRL 547

[Close]

BLAST of CmoCh01G020610 vs. NCBI nr
Match: KAG7037939.1 (4-coumarate--CoA ligase-like 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1030.8 bits (2664), Expect = 4.7e-297
Identity = 539/597 (90.28%), Postives = 543/597 (90.95%), Query Frame = 0


Query: 1   MAEHGGEIDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDATTGQ 60
           MAEHGGEIDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDATTGQ
Sbjct: 1   MAEHGGEIDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDATTGQ 60

Query: 61  HITYSHLWESVDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLN 120
           HITYSHLWE+VDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLN
Sbjct: 61  HITYSHLWEAVDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLN 120

Query: 121 TPQEIAKQIADSKPILAFTTQQLLPKIASSKLPIVLIDGEIQTPSVKIVSTLNEMMRKKP 180
           TPQEIAKQIADSKPILAFTTQQLLPKIASSKLPIVLID +IQTPSVKIV TLNEMMRKKP
Sbjct: 121 TPQEIAKQIADSKPILAFTTQQLLPKIASSKLPIVLIDSQIQTPSVKIVFTLNEMMRKKP 180

Query: 181 SESRIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEGTFICT 240
           SESRIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEGTFICT
Sbjct: 181 SESRIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEGTFICT 240

Query: 241 VPMFHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIDKYRATYLPLVPPILVALVNAA 300
           VPMFHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAI+KYRATYLPLVPPILVALVNAA
Sbjct: 241 VPMFHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIEKYRATYLPLVPPILVALVNAA 300

Query: 301 EQIKGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTDSLEE 360
           EQIKGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTDSLEE
Sbjct: 301 EQIKGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTDSLEE 360

Query: 361 SRRYGTAGLLSPSTEAMIVDPETGKPLPVNRTGELWLRGPTVMKEYRVSFQKNNSGSYGY 420
           SRRYGTAGLLSPSTEAMIVDPETG+PLPVNRTGELWLRGPTVMK              GY
Sbjct: 361 SRRYGTAGLLSPSTEAMIVDPETGEPLPVNRTGELWLRGPTVMK--------------GY 420

Query: 421 FGNVEATASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEALLLTH 480
           FGNVEATASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEALLLTH
Sbjct: 421 FGNVEATASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEALLLTH 480

Query: 481 PNISDAAVIPFPDKEVGQYPMAYVVGKGGSDISHEDVMQFAAKQVKIKFLLFKKWLIIII 540
           PNISDAAVIPFPDKEVGQYPMAYVV KGGSDISHEDVMQF AK                 
Sbjct: 481 PNISDAAVIPFPDKEVGQYPMAYVVRKGGSDISHEDVMQFVAK----------------- 540

Query: 541 ILSGQLYNHKLRRGPRVLVQVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATSRL 598
                              QVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATSRL
Sbjct: 541 -------------------QVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATSRL 547

[Close]

BLAST of CmoCh01G020610 vs. NCBI nr
Match: XP_023522465.1 (4-coumarate--CoA ligase-like 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1030.0 bits (2662), Expect = 8.0e-297
Identity = 539/597 (90.28%), Postives = 543/597 (90.95%), Query Frame = 0


Query: 1   MAEHGGEIDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDATTGQ 60
           MAEHGGEIDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDATTGQ
Sbjct: 1   MAEHGGEIDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDATTGQ 60

Query: 61  HITYSHLWESVDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLN 120
           HITYSHLWE+VDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLN
Sbjct: 61  HITYSHLWEAVDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLN 120

Query: 121 TPQEIAKQIADSKPILAFTTQQLLPKIASSKLPIVLIDGEIQTPSVKIVSTLNEMMRKKP 180
           TPQEIAKQIADSKPILAFTTQQLLPKIASSKLPIVLIDGEI TPSVKIVSTLNEMMRKKP
Sbjct: 121 TPQEIAKQIADSKPILAFTTQQLLPKIASSKLPIVLIDGEIHTPSVKIVSTLNEMMRKKP 180

Query: 181 SESRIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEGTFICT 240
           SESRIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEGTFICT
Sbjct: 181 SESRIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEGTFICT 240

Query: 241 VPMFHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIDKYRATYLPLVPPILVALVNAA 300
           VPMFHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAI+KYRATYLPLVPPILVALVNAA
Sbjct: 241 VPMFHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIEKYRATYLPLVPPILVALVNAA 300

Query: 301 EQIKGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTDSLEE 360
           EQIKGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTDSLEE
Sbjct: 301 EQIKGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTDSLEE 360

Query: 361 SRRYGTAGLLSPSTEAMIVDPETGKPLPVNRTGELWLRGPTVMKEYRVSFQKNNSGSYGY 420
           SRRYGTAGLLSPSTEAMIV+PETG+ LPVNRTGELWLRGPTVMK              GY
Sbjct: 361 SRRYGTAGLLSPSTEAMIVEPETGEALPVNRTGELWLRGPTVMK--------------GY 420

Query: 421 FGNVEATASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEALLLTH 480
           FGNVEATASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEALLLTH
Sbjct: 421 FGNVEATASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEALLLTH 480

Query: 481 PNISDAAVIPFPDKEVGQYPMAYVVGKGGSDISHEDVMQFAAKQVKIKFLLFKKWLIIII 540
           PNISDAAVIPFPDKEVGQYPMAYVV KGGSDISHEDVMQF AK                 
Sbjct: 481 PNISDAAVIPFPDKEVGQYPMAYVVRKGGSDISHEDVMQFVAK----------------- 540

Query: 541 ILSGQLYNHKLRRGPRVLVQVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATSRL 598
                              QVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATSRL
Sbjct: 541 -------------------QVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATSRL 547

[Close]

BLAST of CmoCh01G020610 vs. NCBI nr
Match: XP_022982315.1 (4-coumarate--CoA ligase-like 5 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1024.6 bits (2648), Expect = 3.4e-295
Identity = 536/597 (89.78%), Postives = 541/597 (90.62%), Query Frame = 0


Query: 1   MAEHGGEIDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDATTGQ 60
           MAEHGGEIDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDATTGQ
Sbjct: 1   MAEHGGEIDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDATTGQ 60

Query: 61  HITYSHLWESVDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLN 120
           HITYSHLWES+DSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLN
Sbjct: 61  HITYSHLWESLDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLN 120

Query: 121 TPQEIAKQIADSKPILAFTTQQLLPKIASSKLPIVLIDGEIQTPSVKIVSTLNEMMRKKP 180
           TPQEIAKQIADSKPILAFTTQQLLPKIASS LPIVLIDG+IQTPSVKIVSTLNEMMRKKP
Sbjct: 121 TPQEIAKQIADSKPILAFTTQQLLPKIASSTLPIVLIDGQIQTPSVKIVSTLNEMMRKKP 180

Query: 181 SESRIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEGTFICT 240
           SESRIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEGTFICT
Sbjct: 181 SESRIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEGTFICT 240

Query: 241 VPMFHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIDKYRATYLPLVPPILVALVNAA 300
           VPMFHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAI+KYRATYLPLVPPILVALVNAA
Sbjct: 241 VPMFHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIEKYRATYLPLVPPILVALVNAA 300

Query: 301 EQIKGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTDSLEE 360
           EQIKGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTDSLEE
Sbjct: 301 EQIKGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTDSLEE 360

Query: 361 SRRYGTAGLLSPSTEAMIVDPETGKPLPVNRTGELWLRGPTVMKEYRVSFQKNNSGSYGY 420
           SRRYGTAGLLSPSTEAMIVDPETG+ LPVNRTGELWLRGPTVMK              GY
Sbjct: 361 SRRYGTAGLLSPSTEAMIVDPETGEALPVNRTGELWLRGPTVMK--------------GY 420

Query: 421 FGNVEATASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEALLLTH 480
           FGNVEATASTLDS GWLRTGDLCYID+DGFIFVVDRLKELIKYKGYQVPPAELEALLL H
Sbjct: 421 FGNVEATASTLDSMGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLAH 480

Query: 481 PNISDAAVIPFPDKEVGQYPMAYVVGKGGSDISHEDVMQFAAKQVKIKFLLFKKWLIIII 540
           PNISDAAVIPFPDKEVGQYPMAYVV KGGSDISHEDVMQF AK                 
Sbjct: 481 PNISDAAVIPFPDKEVGQYPMAYVVRKGGSDISHEDVMQFVAK----------------- 540

Query: 541 ILSGQLYNHKLRRGPRVLVQVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATSRL 598
                              QVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATSRL
Sbjct: 541 -------------------QVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATSRL 547

[Close]

BLAST of CmoCh01G020610 vs. NCBI nr
Match: KAG6608623.1 (4-coumarate--CoA ligase-like 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1005.0 bits (2597), Expect = 2.8e-289
Identity = 524/581 (90.19%), Postives = 528/581 (90.88%), Query Frame = 0


Query: 1   MAEHGGEIDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDATTGQ 60
           MAEHGGEIDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDATTGQ
Sbjct: 1   MAEHGGEIDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDATTGQ 60

Query: 61  HITYSHLWESVDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLN 120
           HITYSHLWE+VDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLN
Sbjct: 61  HITYSHLWEAVDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLN 120

Query: 121 TPQEIAKQIADSKPILAFTTQQLLPKIASSKLPIVLIDGEIQTPSVKIVSTLNEMMRKKP 180
           TPQEIAKQIADSKPILAFTTQQLLPKIASSKLPIVLID +IQTPSVKIVSTLNEMMRKKP
Sbjct: 121 TPQEIAKQIADSKPILAFTTQQLLPKIASSKLPIVLIDSQIQTPSVKIVSTLNEMMRKKP 180

Query: 181 SESRIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEGTFICT 240
           SESRIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEGTFICT
Sbjct: 181 SESRIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEGTFICT 240

Query: 241 VPMFHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIDKYRATYLPLVPPILVALVNAA 300
           VPMFHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAI+KYRATYLPLVPPILVALVNAA
Sbjct: 241 VPMFHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIEKYRATYLPLVPPILVALVNAA 300

Query: 301 EQIKGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTDSLEE 360
           EQIKGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTDSLEE
Sbjct: 301 EQIKGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTDSLEE 360

Query: 361 SRRYGTAGLLSPSTEAMIVDPETGKPLPVNRTGELWLRGPTVMKEYRVSFQKNNSGSYGY 420
           SRRYGTAGLLSPSTEAMIVDPETG+PLPVNRTGELWLRGPTVMK              GY
Sbjct: 361 SRRYGTAGLLSPSTEAMIVDPETGEPLPVNRTGELWLRGPTVMK--------------GY 420

Query: 421 FGNVEATASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEALLLTH 480
           FGNVEATASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEALLLTH
Sbjct: 421 FGNVEATASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEALLLTH 480

Query: 481 PNISDAAVIPFPDKEVGQYPMAYVVGKGGSDISHEDVMQFAAKQVKIKFLLFKKWLIIII 540
           PNISDAAVIPFPDKEVGQYPMAYVV KGGSDISHEDVMQF AK                 
Sbjct: 481 PNISDAAVIPFPDKEVGQYPMAYVVRKGGSDISHEDVMQFVAK----------------- 531

Query: 541 ILSGQLYNHKLRRGPRVLVQVAPYKRIRRVAFVDSIPKNPS 582
                              QVAPYKRIRRVAFVDSIPKNPS
Sbjct: 541 -------------------QVAPYKRIRRVAFVDSIPKNPS 531

[Close]

BLAST of CmoCh01G020610 vs. TAIR 10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 778.1 bits (2008), Expect = 5.1e-225
Identity = 406/590 (68.81%), Postives = 464/590 (78.64%), Query Frame = 0


Query: 8   IDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDATTGQHITYSHL 67
           ++ RSGFC S   F+SKR PIPLPPN SLD TTFISS+ H G+IA IDA+TGQ++T++ L
Sbjct: 4   VNSRSGFCNSNSTFYSKRTPIPLPPNPSLDVTTFISSQAHRGRIAFIDASTGQNLTFTEL 63

Query: 68  WESVDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLNTPQEIAK 127
           W +V+SVA  LS++GIRKGHV+LLLSPNSI FP++CL+VMS+GAIITTTNPLNT  EIAK
Sbjct: 64  WRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNEIAK 123

Query: 128 QIADSKPILAFTTQQLLPKI--ASSKLPIVLIDGEIQTPSVKIVSTLNEMMRKKPSESRI 187
           QI DS P+LAFTT QLLPKI  A+ KLPIVL+D E +  SV  V  L EMM+K+PS +R+
Sbjct: 124 QIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEE-RVDSVGDVRRLVEMMKKEPSGNRV 183

Query: 188 KERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEGTFICTVPMFH 247
           KERV+Q+DTATLLYSSGTTG SKGV+SSH+NLIAMVQ +V RF   +GE  FICTVPMFH
Sbjct: 184 KERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQRFICTVPMFH 243

Query: 248 IYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIDKYRATYLPLVPPILVALVNAAEQIKG 307
           IYGL AFATGLL+ GSTIIVLSKFE+HEM+SAI KY+AT LPLVPPILVA+VN A+QIK 
Sbjct: 244 IYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNGADQIKA 303

Query: 308 KYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTDSLEESRRYG 367
           KYDLSS+HT L GGAPL KEV EGF EKYP V ILQGYGLTESTGIGASTD++EESRRYG
Sbjct: 304 KYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVEESRRYG 363

Query: 368 TAGLLSPSTEAMIVDPETGKPLPVNRTGELWLRGPTVMKEYRVSFQKNNSGSYGYFGNVE 427
           TAG LS S E  IVDP TG+ L   +TGELWL+GP++MK              GYF N E
Sbjct: 364 TAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMK--------------GYFSNEE 423

Query: 428 ATASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNISD 487
           AT+STLDS+GWLRTGDLCYID+DGFIFVVDRLKELIKYKGYQV PAELEALLLTHP I+D
Sbjct: 424 ATSSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITD 483

Query: 488 AAVIPFPDKEVGQYPMAYVVGKGGSDISHEDVMQFAAKQVKIKFLLFKKWLIIIIILSGQ 547
           AAVIPFPDKEVGQ+PMAYVV K GS +S + +M+F AK                      
Sbjct: 484 AAVIPFPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAK---------------------- 542

Query: 548 LYNHKLRRGPRVLVQVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATS 596
                         QVAPYKRIR+VAFV SIPKNPSGKILRKDLIK+ATS
Sbjct: 544 --------------QVAPYKRIRKVAFVSSIPKNPSGKILRKDLIKIATS 542

[Close]

BLAST of CmoCh01G020610 vs. TAIR 10
Match: AT1G20510.2 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 684.9 bits (1766), Expect = 5.9e-197
Identity = 351/485 (72.37%), Postives = 402/485 (82.89%), Query Frame = 0


Query: 8   IDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDATTGQHITYSHL 67
           ++ RSGFC S   F+SKR PIPLPPN SLD TTFISS+ H G+IA IDA+TGQ++T++ L
Sbjct: 4   VNSRSGFCNSNSTFYSKRTPIPLPPNPSLDVTTFISSQAHRGRIAFIDASTGQNLTFTEL 63

Query: 68  WESVDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLNTPQEIAK 127
           W +V+SVA  LS++GIRKGHV+LLLSPNSI FP++CL+VMS+GAIITTTNPLNT  EIAK
Sbjct: 64  WRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNEIAK 123

Query: 128 QIADSKPILAFTTQQLLPKI--ASSKLPIVLIDGEIQTPSVKIVSTLNEMMRKKPSESRI 187
           QI DS P+LAFTT QLLPKI  A+ KLPIVL+D E +  SV  V  L EMM+K+PS +R+
Sbjct: 124 QIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEE-RVDSVGDVRRLVEMMKKEPSGNRV 183

Query: 188 KERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEGTFICTVPMFH 247
           KERV+Q+DTATLLYSSGTTG SKGV+SSH+NLIAMVQ +V RF   +GE  FICTVPMFH
Sbjct: 184 KERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQRFICTVPMFH 243

Query: 248 IYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIDKYRATYLPLVPPILVALVNAAEQIKG 307
           IYGL AFATGLL+ GSTIIVLSKFE+HEM+SAI KY+AT LPLVPPILVA+VN A+QIK 
Sbjct: 244 IYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNGADQIKA 303

Query: 308 KYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTDSLEESRRYG 367
           KYDLSS+HT L GGAPL KEV EGF EKYP V ILQGYGLTESTGIGASTD++EESRRYG
Sbjct: 304 KYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVEESRRYG 363

Query: 368 TAGLLSPSTEAMIVDPETGKPLPVNRTGELWLRGPTVMKEYRVSFQKNNSGSYGYFGNVE 427
           TAG LS S E  IVDP TG+ L   +TGELWL+GP++MK              GYF N E
Sbjct: 364 TAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMK--------------GYFSNEE 423

Query: 428 ATASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNISD 487
           AT+STLDS+GWLRTGDLCYID+DGFIFVVDRLKELIKYKGYQV PAELEALLLTHP I+D
Sbjct: 424 ATSSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITD 473

Query: 488 AAVIP 491
           AAVIP
Sbjct: 484 AAVIP 473

[Close]

BLAST of CmoCh01G020610 vs. TAIR 10
Match: AT1G20500.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 659.4 bits (1700), Expect = 2.7e-189
Identity = 340/594 (57.24%), Postives = 430/594 (72.39%), Query Frame = 0


Query: 8   IDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDATTGQHITYSHL 67
           +DPRSGFCKS   F+SKR P+ LPPN S D TTFISS+PH GK A IDA TGQ +T+S L
Sbjct: 10  VDPRSGFCKSNSTFYSKRQPLSLPPNLSRDVTTFISSQPHRGKTAFIDAATGQCLTFSDL 69

Query: 68  WESVDSVASAL-SDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLNTPQEIA 127
           W +VD VA  L  ++GIR+G V+L+LSPNSIF P++CL+VMS+GA+ TT N LNT  EI+
Sbjct: 70  WRAVDRVADCLYHEVGIRRGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTLNTSGEIS 129

Query: 128 KQIADSKPILAFTTQQLLPKIASSKLPIVLIDGEIQ---TPSVKIVSTLNEMMRKKPSES 187
           KQIADS P L FTT+QL PK+  + + +VL D E+    T ++++V  L+EM++K+PS  
Sbjct: 130 KQIADSNPTLVFTTRQLAPKLPVA-ISVVLTDDEVYQELTSAIRVVGILSEMVKKEPSGQ 189

Query: 188 RIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEGTFICTVPM 247
           R+++RV Q+DTA +LYSSGTTG SKGV+SSH+NL A V   ++     + +  FICTVPM
Sbjct: 190 RVRDRVNQDDTAMMLYSSGTTGPSKGVISSHRNLTAHVARFIS--DNLKRDDIFICTVPM 249

Query: 248 FHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIDKYRATYLPLVPPILVALVNAAEQI 307
           FH YGL+ FA G ++ GST+++L +F++H+M+ A++K+RAT L L PP+LVA++N A+ I
Sbjct: 250 FHTYGLLTFAMGTVALGSTVVILRRFQLHDMMDAVEKHRATALALAPPVLVAMINDADLI 309

Query: 308 KGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTDSLEESRR 367
           K KYDLSSL T   GGAPL KEV EGF+EKYP V ILQGY LTES G GA T+S EESRR
Sbjct: 310 KAKYDLSSLKTVRCGGAPLSKEVTEGFLEKYPTVDILQGYALTESNGGGAFTNSAEESRR 369

Query: 368 YGTAGLLSPSTEAMIVDPETGKPLPVNRTGELWLRGPTVMKEYRVSFQKNNSGSYGYFGN 427
           YGTAG L+   EA IVDP TG+ + +N+TGELWL+GP++ K              GYF N
Sbjct: 370 YGTAGTLTSDVEARIVDPNTGRFMGINQTGELWLKGPSISK--------------GYFKN 429

Query: 428 VEATASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNI 487
            EAT  T++ +GWL+TGDLCYID+DGF+FVVDRLKELIKYKGYQVPPAELEALL+THP+I
Sbjct: 430 QEATNETINLEGWLKTGDLCYIDEDGFLFVVDRLKELIKYKGYQVPPAELEALLITHPDI 489

Query: 488 SDAAVIPFPDKEVGQYPMAYVVGKGGSDISHEDVMQFAAKQVKIKFLLFKKWLIIIIILS 547
            DAAVIPFPDKE GQYPMAYVV K  S++S + V+ F +K                    
Sbjct: 490 LDAAVIPFPDKEAGQYPMAYVVRKHESNLSEKQVIDFISK-------------------- 549

Query: 548 GQLYNHKLRRGPRVLVQVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATSRL 598
                           QVAPYK+IR+V+F++SIPK  SGK LRKDLIKLATS+L
Sbjct: 550 ----------------QVAPYKKIRKVSFINSIPKTASGKTLRKDLIKLATSKL 550

[Close]

BLAST of CmoCh01G020610 vs. TAIR 10
Match: AT1G20480.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 651.7 bits (1680), Expect = 5.5e-187
Identity = 339/599 (56.59%), Postives = 431/599 (71.95%), Query Frame = 0


Query: 8   IDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDATTGQHITYSHL 67
           +D +SGFC+ST IF+SKR P+ LPPNQ LD T+FI+S+PH GK   +DA TG+ +++  L
Sbjct: 21  VDRKSGFCESTSIFYSKREPMALPPNQFLDVTSFIASQPHRGKTVFVDAVTGRRLSFPEL 80

Query: 68  WESVDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLNTPQEIAK 127
           W  V+ VA  L  +G+RKG+V+++LSPNSI FPI+ L+VMS+GAIITT NP+NT  EI+K
Sbjct: 81  WLGVERVAGCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPINTSDEISK 140

Query: 128 QIADSKPILAFTTQQLLPKIASS---KLPIVLIDGEIQTPS------VKIVSTLNEMMRK 187
           QI DS+P+LAFTT +L+ K+A++    LP+VL+D +   PS      VK+V  L  M+  
Sbjct: 141 QIGDSRPVLAFTTCKLVSKLAAASNFNLPVVLMD-DYHVPSQSYGDRVKLVGRLETMIET 200

Query: 188 KPSESRIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEGTFI 247
           +PSESR+K+RV Q+DTA LLYSSGTTG SKGV+ SH+NLIA+VQ    RF L   E   I
Sbjct: 201 EPSESRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIALVQAYRARFGL---EQRTI 260

Query: 248 CTVPMFHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIDKYRATYLPLVPPILVALVN 307
           CT+PM HI+G   FATGL++ G TI+VL KF++ ++LSA++ +R++YL LVPPI+VA+VN
Sbjct: 261 CTIPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYLSLVPPIVVAMVN 320

Query: 308 AAEQIKGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTDSL 367
            A +I  KYDLSSLHT ++GGAPL +EV E FVE YP V ILQGYGLTEST I AS  + 
Sbjct: 321 GANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYGLTESTAIAASMFNK 380

Query: 368 EESRRYGTAGLLSPSTEAMIVDPETGKPLPVNRTGELWLRGPTVMKEYRVSFQKNNSGSY 427
           EE++RYG +GLL+P+ E  IVDP+TG+ L VN+TGELW+R PTVMK              
Sbjct: 381 EETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMK-------------- 440

Query: 428 GYFGNVEATASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEALLL 487
           GYF N EATAST+DS+GWL+TGDLCYID DGF+FVVDRLKELIK  GYQV PAELEALLL
Sbjct: 441 GYFKNKEATASTIDSEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLL 500

Query: 488 THPNISDAAVIPFPDKEVGQYPMAYVVGKGGSDISHEDVMQFAAKQVKIKFLLFKKWLII 547
            HP I+DAAVIP PD + GQYPMAY+V K GS++S  ++M F AK               
Sbjct: 501 AHPEIADAAVIPIPDMKAGQYPMAYIVRKVGSNLSESEIMGFVAK--------------- 560

Query: 548 IIILSGQLYNHKLRRGPRVLVQVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATSRL 598
                                QV+PYK+IR+V F+ SIPKNPSGKILR++L KL TS+L
Sbjct: 561 ---------------------QVSPYKKIRKVTFLASIPKNPSGKILRRELTKLTTSKL 565

[Close]

BLAST of CmoCh01G020610 vs. TAIR 10
Match: AT5G38120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 615.5 bits (1586), Expect = 4.4e-176
Identity = 324/594 (54.55%), Postives = 412/594 (69.36%), Query Frame = 0


Query: 8   IDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDATTGQHITYSHL 67
           IDPR+GFC S   F+SKR P+ LP  +SLD TTFISS+ + GK A IDA T   I++S L
Sbjct: 11  IDPRNGFCTSNSTFYSKRKPLALPSKESLDITTFISSQTYRGKTAFIDAATDHRISFSDL 70

Query: 68  WESVDSVASA-LSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLNTPQEIA 127
           W +VD VA   L D+GIR+G V+L+LSPN+I  PI+CL+VMS+GA++TT NPLNT  EI 
Sbjct: 71  WMAVDRVADCLLHDVGIRRGDVVLVLSPNTISIPIVCLSVMSLGAVLTTANPLNTASEIL 130

Query: 128 KQIADSKPILAFTTQQLLPKIASSKLPIVL--IDGEIQTP-SVKIVSTLNEMMRKKPSES 187
           +QIADS P LAFTT +L PKIASS + IVL  ++  ++ P  +K+V  L EMM+K+PS  
Sbjct: 131 RQIADSNPKLAFTTPELAPKIASSGISIVLERVEDTLRVPRGLKVVGNLTEMMKKEPSGQ 190

Query: 188 RIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEGEGTFICTVPM 247
            ++ +V ++DTA LLYSSGTTG SKGV SSH NLIA V   +      + + TFICTVP+
Sbjct: 191 AVRNQVHKDDTAMLLYSSGTTGRSKGVNSSHGNLIAHVARYIAE-PFEQPQQTFICTVPL 250

Query: 248 FHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIDKYRATYLPLVPPILVALVNAAEQI 307
           FH +GL+ F    L+ G+T+++L +F++ EM++A++KYRAT L LVPP+LV ++N A+QI
Sbjct: 251 FHTFGLLNFVLATLALGTTVVILPRFDLGEMMAAVEKYRATTLILVPPVLVTMINKADQI 310

Query: 308 KGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGASTDSLEESRR 367
             KYD+S L T   GGAPL KEV +GF++KYP V + QGY LTES G GAS +S+EESRR
Sbjct: 311 MKKYDVSFLRTVRCGGAPLSKEVTQGFMKKYPTVDVYQGYALTESNGAGASIESVEESRR 370

Query: 368 YGTAGLLSPSTEAMIVDPETGKPLPVNRTGELWLRGPTVMKEYRVSFQKNNSGSYGYFGN 427
           YG  GLLS   EA IVDP TG+ + +N+TGELWL+GP++ K              GYF N
Sbjct: 371 YGAVGLLSCGVEARIVDPNTGQVMGLNQTGELWLKGPSIAK--------------GYFRN 430

Query: 428 VEATASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNI 487
            E     + S+GWL+TGDLCYID DGF+F+VDRLKELIKYKGYQVPPAELEALLL HP+I
Sbjct: 431 EE---EIITSEGWLKTGDLCYIDNDGFLFIVDRLKELIKYKGYQVPPAELEALLLNHPDI 490

Query: 488 SDAAVIPFPDKEVGQYPMAYVVGKGGSDISHEDVMQFAAKQVKIKFLLFKKWLIIIIILS 547
            DAAVIPFPDKE GQ+PMAYV  K  S++  + V+ F +K                    
Sbjct: 491 LDAAVIPFPDKEAGQFPMAYVARKPESNLCEKKVIDFISK-------------------- 550

Query: 548 GQLYNHKLRRGPRVLVQVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATSRL 598
                           QVAPYK+IR+VAF+DSIPK PSGK LRKDLIK A S++
Sbjct: 551 ----------------QVAPYKKIRKVAFIDSIPKTPSGKTLRKDLIKFAISKI 550

The following BLAST results are available for this feature:

BLAST of CmoCh01G020610 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
M4IQR7	5.5e-232	70.49	Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1	[more]
Q84P21	7.2e-224	68.81	4-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana OX=3702 GN=4CLL5 PE=1 SV=...	[more]
Q10S72	4.6e-194	58.74	4-coumarate--CoA ligase-like 4 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL4...	[more]
P0C5B6	3.7e-188	57.24	OPC-6:CoA ligase OS=Arabidopsis thaliana OX=3702 GN=4CLL4 PE=2 SV=1	[more]
Q84P25	7.8e-186	56.59	4-coumarate--CoA ligase-like 2 OS=Arabidopsis thaliana OX=3702 GN=4CLL2 PE=2 SV=...	[more]

BLAST of CmoCh01G020610 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FMD2	8.9e-302	91.62	4-coumarate--CoA ligase-like 5 OS=Cucurbita moschata OX=3662 GN=LOC111446530 PE=...	[more]
A0A6J1J2B8	1.6e-295	89.78	4-coumarate--CoA ligase-like 5 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1F7P0	6.6e-281	84.39	4-coumarate--CoA ligase-like 5 OS=Cucurbita moschata OX=3662 GN=LOC111443102 PE=...	[more]
A0A6J1II10	2.4e-278	83.89	4-coumarate--CoA ligase-like 5 OS=Cucurbita maxima OX=3661 GN=LOC111476465 PE=4 ...	[more]
A0A0A0L385	9.3e-275	83.17	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G653450 PE=4 SV=1	[more]

BLAST of CmoCh01G020610 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022941144.1	1.8e-301	91.62	4-coumarate--CoA ligase-like 5 [Cucurbita moschata]	[more]
KAG7037939.1	4.7e-297	90.28	4-coumarate--CoA ligase-like 5, partial [Cucurbita argyrosperma subsp. argyrospe...	[more]
XP_023522465.1	8.0e-297	90.28	4-coumarate--CoA ligase-like 5 [Cucurbita pepo subsp. pepo]	[more]
XP_022982315.1	3.4e-295	89.78	4-coumarate--CoA ligase-like 5 isoform X1 [Cucurbita maxima]	[more]
KAG6608623.1	2.8e-289	90.19	4-coumarate--CoA ligase-like 5, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of CmoCh01G020610 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G20510.1	5.1e-225	68.81	OPC-8:0 CoA ligase1	[more]
AT1G20510.2	5.9e-197	72.37	OPC-8:0 CoA ligase1	[more]
AT1G20500.1	2.7e-189	57.24	AMP-dependent synthetase and ligase family protein	[more]
AT1G20480.1	5.5e-187	56.59	AMP-dependent synthetase and ligase family protein	[more]
AT5G38120.1	4.4e-176	54.55	AMP-dependent synthetase and ligase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR025110	AMP-binding enzyme, C-terminal domain	PFAM	PF13193	AMP-binding_C	coord: 472..583 e-value: 9.5E-12 score: 45.7
IPR000873	AMP-dependent synthetase/ligase	PFAM	PF00501	AMP-binding	coord: 47..463 e-value: 7.2E-98 score: 328.0
None	No IPR available	GENE3D	3.30.300.30		coord: 459..596 e-value: 2.9E-28 score: 100.5
None	No IPR available	PANTHER	PTHR24096:SF339	4-COUMARATE--COA LIGASE-LIKE 3-RELATED	coord: 559..596
None	No IPR available	PANTHER	PTHR24096	LONG-CHAIN-FATTY-ACID--COA LIGASE	coord: 9..526
None	No IPR available	PANTHER	PTHR24096:SF339	4-COUMARATE--COA LIGASE-LIKE 3-RELATED	coord: 9..526
None	No IPR available	PANTHER	PTHR24096	LONG-CHAIN-FATTY-ACID--COA LIGASE	coord: 559..596
None	No IPR available	CDD	cd05904	4CL	coord: 30..589 e-value: 0.0 score: 777.183
None	No IPR available	SUPERFAMILY	56801	Acetyl-CoA synthetase-like	coord: 42..595
IPR042099	ANL, N-terminal domain	GENE3D	3.40.50.12780		coord: 18..458 e-value: 1.0E-121 score: 408.7
IPR020845	AMP-binding, conserved site	PROSITE	PS00455	AMP_BINDING	coord: 197..208

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh01G020610.1	CmoCh01G020610.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009698	phenylpropanoid metabolic process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005777	peroxisome
molecular_function	GO:0016207	4-coumarate-CoA ligase activity