CmoCh01G020580 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh01G020580
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionL-ascorbate oxidase homolog
LocationCmo_Chr01: 14373346 .. 14375853 (-)
RNA-Seq ExpressionCmoCh01G020580
SyntenyCmoCh01G020580
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCCACTAATCCAAAATGCTGCTTCTAACTTCACCGGCCTTCGCCCTCTCTGCATTCCTCTGCTTCTTCGCCGCCGTCTCTGCAGACGATCCTTACCGTTTCTTCACCTGGAATGTCTCCTACGGCGACATTTACCCTCTCGGCCTCCGCCAGCAGGTTCCTTCTTTCTTCTCCCTTTTTCCTACTCTCTTTGATCTCCTCGTTAATGCCGCCCCTCATCTGTTCTTCTTCTGTTCTTCTTCTGCAGGGCATACTGATCAATGGTCAGTTCCCCGGCCCTGATATCCACTGCGTCACTAACGACAACCTTATTATCAATGTCTTCAACAGCTTAGATGAACCCTTCCTCATTTCCTGGTCATTATTTCTACTCTCCATTTCACTTGTTTTTATTTTACTTTTAACCCTGTTTTCATGTCCCCCAACTCCAACTCCAACTCCAACTCCAACTCCAACTCTGCAACGCAGGAACGGAGTTCAACAGAGAAAGAACTCATACGAAGACGGTGTTTATGGAACAACCTGCCCCATCCCTCCCGGAAAGAACTTCACCTACATTCTCCAAGTCAAGGATCAGATTGGAAGCTTTTACTATTTCCCCTCTCTCGCCTTCCACAAGGCCGCCGGTGGTTTCGGCGCCATCAGAATCCTCAGCCGCCCCCTCATCCCCGTCCCCTTCCCTGATCCCGCCGGTGACTACTCCCTTCTCATCGGAGACTGGTACAGCTCCAATCACACGGTATTCATTATCTTTGCTGCTTCTTTATTTTTCCCCAAATGGGTCGAATGCTTTTTATTTCTTTTTTTAATTTGAGCTGTGAAATTTGGCGCATAGACCTTGAAGGCTTGTTTGGATCTTGGTAAAAAGCTTCCATTTCCTGATGGAATCCTCATCAATGGCCGTGCCAATTACACCTCCTTCAGCGTTGAACAAGGTATAATCCCCGGTCTAATTTGATTGGCGATTCTAAGGCCAATGGCTATCCAATTGGACTGAGATGATCATTCATGTCTATGCTGTTGCAGGAAAAACTTACAGGTTGAGAATTTCAAATGTGGGGCTGCAGCATTCCCTCAACTTCCGAATTCAAGGACATCAAATGAAGCTTGTAGAAGTGGAGGGAACACATACCCTTCAAACCACGTACTCCTCGCTCGACATTCATGTCGGTCAGTCTTACTCCGTGCTCGTCACAGCCGACCAGCCAGCTCAAGACTACTACATTGTCGTCTCTTCTCGCTTCACCAACCCAGTGCTCACCACCACCGGCACTCTTCGTTACAGTAACTCTACTGGCCCTGTCAAAGGCCCTCTTCCTGGAGCTCCAACCACAGAAGTCGGCTGGTCTCTCAACCAAGCTCGCTCTATTAGGTGATAACCAGACCAAATGGTTAGTCTGTGATTTTCCATATCTTGCTATAGTCTTGATTATGGCGTTTTTGTGTTTGCAGGACCAACCTTACAGCCAGTGGACCAAGGCCAAACCCACAAGGATCATATCACTATGGTCTGGTAAACACAAGCAGAACCATCATACTTGAAAGTTCTGCTGGCCTAATCAACAGCAAACAAAGATATGCGGTTAACAGAGTCTCCTTCATCCCGGCTGACACTCCCCTGAAACTTGCCGACTTCTTCAAAATAGGAGGGGTTTTTCGCGTCGGAAGTATCTCCGATCGCCCTACCGGTGGAGGAATGTACCTCGACACATCAGTGATGGGTGCAGACTACAGAGCGTTTGTTGAAATTGTGTTCCAAAACAATGAGAACATTGTCCAAAGTTGGCATATTGATGGCTACTCTTTCTTTGTGGTCGGGTAAGCTCACATATGTTTGTGTGGATATCTGATGAATCAATATAGGATGTTTCTGAAAGTGTTGTGTTTGTCAGTATGGATGGAGGGCAGTGGAGTGAGTCTAGCAGAGGGCAGTACAATCTGCGGGATGCAATAGCTCGTTGTACGATTCAGGTGAGCATCGAGTTTGGATTCCCTTTTGGCTTTTAGGCTCGTAAATGATGTGATGGTGTTTGGGGCAGGTATACCCTTTGTCATGGACTGCAATTTACATGTCCCTGGACAACGTAGGGATGTGGAACATGAGGTCGGAGTTTTGGGCTAGACAGTATCTGGGACAGCAATTCTACCTCCGCGTGTATACGCCATCGACGTCGCTTAGGGACGAGTACCCCATTCCAAAGAATGCTCTTCTGTGTGGCAGGGCAAGTGGTAGGCACACCAGGCCACTCTAAATCAGTAGCATTCCAAATATTTGAACCCCACACACACACATACACATACACACACAAGGTGGCTAAAAAGCAAGGTCTGTTTGCTTTCGTAGTGTTGTAATATCGATGATTAGATTGCCCGGTCCTTCGATTGACCCTATCTCGTGGTCGGCTCACATTCTTTCCCCGTATATCATTTGATTTCTTCCCCCATTTGCTCTTTTTATTTTTATATCATATTGTTACC

mRNA sequence

TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCCACTAATCCAAAATGCTGCTTCTAACTTCACCGGCCTTCGCCCTCTCTGCATTCCTCTGCTTCTTCGCCGCCGTCTCTGCAGACGATCCTTACCGTTTCTTCACCTGGAATGTCTCCTACGGCGACATTTACCCTCTCGGCCTCCGCCAGCAGGGCATACTGATCAATGGTCAGTTCCCCGGCCCTGATATCCACTGCGTCACTAACGACAACCTTATTATCAATGTCTTCAACAGCTTAGATGAACCCTTCCTCATTTCCTGGTCATTATTTCTACTCTCCATTTCACTTGTTTTTATTTTACTTTTAACCCTGTTTTCATGTCCCCCAACTCCAACTCCAACTCCAACTCCAACTCCAACTCTGCAACGCAGGAACGGAGTTCAACAGAGAAAGAACTCATACGAAGACGGTGTTTATGGAACAACCTGCCCCATCCCTCCCGGAAAGAACTTCACCTACATTCTCCAAGTCAAGGATCAGATTGGAAGCTTTTACTATTTCCCCTCTCTCGCCTTCCACAAGGCCGCCGGTGGTTTCGGCGCCATCAGAATCCTCAGCCGCCCCCTCATCCCCGTCCCCTTCCCTGATCCCGCCGGTGACTACTCCCTTCTCATCGGAGACTGGTACAGCTCCAATCACACGACCTTGAAGGCTTGTTTGGATCTTGGTAAAAAGCTTCCATTTCCTGATGGAATCCTCATCAATGGCCGTGCCAATTACACCTCCTTCAGCGTTGAACAAGGAAAAACTTACAGGTTGAGAATTTCAAATGTGGGGCTGCAGCATTCCCTCAACTTCCGAATTCAAGGACATCAAATGAAGCTTGTAGAAGTGGAGGGAACACATACCCTTCAAACCACGTACTCCTCGCTCGACATTCATGTCGGTCAGTCTTACTCCGTGCTCGTCACAGCCGACCAGCCAGCTCAAGACTACTACATTGTCGTCTCTTCTCGCTTCACCAACCCAGTGCTCACCACCACCGGCACTCTTCGTTACAGTAACTCTACTGGCCCTGTCAAAGGCCCTCTTCCTGGAGCTCCAACCACAGAAGTCGGCTGGTCTCTCAACCAAGCTCGCTCTATTAGGACCAACCTTACAGCCAGTGGACCAAGGCCAAACCCACAAGGATCATATCACTATGGTCTGGTAAACACAAGCAGAACCATCATACTTGAAAGTTCTGCTGGCCTAATCAACAGCAAACAAAGATATGCGGTTAACAGAGTCTCCTTCATCCCGGCTGACACTCCCCTGAAACTTGCCGACTTCTTCAAAATAGGAGGGGTTTTTCGCGTCGGAAGTATCTCCGATCGCCCTACCGGTGGAGGAATGTACCTCGACACATCAGTGATGGGTGCAGACTACAGAGCGTTTGTTGAAATTGTGTTCCAAAACAATGAGAACATTGTCCAAAGTTGGCATATTGATGGCTACTCTTTCTTTGTGGTCGGTATGGATGGAGGGCAGTGGAGTGAGTCTAGCAGAGGGCAGTACAATCTGCGGGATGCAATAGCTCGTTGTACGATTCAGGTATACCCTTTGTCATGGACTGCAATTTACATGTCCCTGGACAACGTAGGGATGTGGAACATGAGGTCGGAGTTTTGGGCTAGACAGTATCTGGGACAGCAATTCTACCTCCGCGTGTATACGCCATCGACGTCGCTTAGGGACGAGTACCCCATTCCAAAGAATGCTCTTCTGTGTGGCAGGGCAAGTGGTAGGCACACCAGGCCACTCTAAATCAGTAGCATTCCAAATATTTGAACCCCACACACACACATACACATACACACACAAGGTGGCTAAAAAGCAAGGTCTGTTTGCTTTCGTAGTGTTGTAATATCGATGATTAGATTGCCCGGTCCTTCGATTGACCCTATCTCGTGGTCGGCTCACATTCTTTCCCCGTATATCATTTGATTTCTTCCCCCATTTGCTCTTTTTATTTTTATATCATATTGTTACC

Coding sequence (CDS)

ATGCTGCTTCTAACTTCACCGGCCTTCGCCCTCTCTGCATTCCTCTGCTTCTTCGCCGCCGTCTCTGCAGACGATCCTTACCGTTTCTTCACCTGGAATGTCTCCTACGGCGACATTTACCCTCTCGGCCTCCGCCAGCAGGGCATACTGATCAATGGTCAGTTCCCCGGCCCTGATATCCACTGCGTCACTAACGACAACCTTATTATCAATGTCTTCAACAGCTTAGATGAACCCTTCCTCATTTCCTGGTCATTATTTCTACTCTCCATTTCACTTGTTTTTATTTTACTTTTAACCCTGTTTTCATGTCCCCCAACTCCAACTCCAACTCCAACTCCAACTCCAACTCTGCAACGCAGGAACGGAGTTCAACAGAGAAAGAACTCATACGAAGACGGTGTTTATGGAACAACCTGCCCCATCCCTCCCGGAAAGAACTTCACCTACATTCTCCAAGTCAAGGATCAGATTGGAAGCTTTTACTATTTCCCCTCTCTCGCCTTCCACAAGGCCGCCGGTGGTTTCGGCGCCATCAGAATCCTCAGCCGCCCCCTCATCCCCGTCCCCTTCCCTGATCCCGCCGGTGACTACTCCCTTCTCATCGGAGACTGGTACAGCTCCAATCACACGACCTTGAAGGCTTGTTTGGATCTTGGTAAAAAGCTTCCATTTCCTGATGGAATCCTCATCAATGGCCGTGCCAATTACACCTCCTTCAGCGTTGAACAAGGAAAAACTTACAGGTTGAGAATTTCAAATGTGGGGCTGCAGCATTCCCTCAACTTCCGAATTCAAGGACATCAAATGAAGCTTGTAGAAGTGGAGGGAACACATACCCTTCAAACCACGTACTCCTCGCTCGACATTCATGTCGGTCAGTCTTACTCCGTGCTCGTCACAGCCGACCAGCCAGCTCAAGACTACTACATTGTCGTCTCTTCTCGCTTCACCAACCCAGTGCTCACCACCACCGGCACTCTTCGTTACAGTAACTCTACTGGCCCTGTCAAAGGCCCTCTTCCTGGAGCTCCAACCACAGAAGTCGGCTGGTCTCTCAACCAAGCTCGCTCTATTAGGACCAACCTTACAGCCAGTGGACCAAGGCCAAACCCACAAGGATCATATCACTATGGTCTGGTAAACACAAGCAGAACCATCATACTTGAAAGTTCTGCTGGCCTAATCAACAGCAAACAAAGATATGCGGTTAACAGAGTCTCCTTCATCCCGGCTGACACTCCCCTGAAACTTGCCGACTTCTTCAAAATAGGAGGGGTTTTTCGCGTCGGAAGTATCTCCGATCGCCCTACCGGTGGAGGAATGTACCTCGACACATCAGTGATGGGTGCAGACTACAGAGCGTTTGTTGAAATTGTGTTCCAAAACAATGAGAACATTGTCCAAAGTTGGCATATTGATGGCTACTCTTTCTTTGTGGTCGGTATGGATGGAGGGCAGTGGAGTGAGTCTAGCAGAGGGCAGTACAATCTGCGGGATGCAATAGCTCGTTGTACGATTCAGGTATACCCTTTGTCATGGACTGCAATTTACATGTCCCTGGACAACGTAGGGATGTGGAACATGAGGTCGGAGTTTTGGGCTAGACAGTATCTGGGACAGCAATTCTACCTCCGCGTGTATACGCCATCGACGTCGCTTAGGGACGAGTACCCCATTCCAAAGAATGCTCTTCTGTGTGGCAGGGCAAGTGGTAGGCACACCAGGCCACTCTAA

Protein sequence

MLLLTSPAFALSAFLCFFAAVSADDPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDIHCVTNDNLIINVFNSLDEPFLISWSLFLLSISLVFILLLTLFSCPPTPTPTPTPTPTLQRRNGVQQRKNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIRILSRPLIPVPFPDPAGDYSLLIGDWYSSNHTTLKACLDLGKKLPFPDGILINGRANYTSFSVEQGKTYRLRISNVGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSVLVTADQPAQDYYIVVSSRFTNPVLTTTGTLRYSNSTGPVKGPLPGAPTTEVGWSLNQARSIRTNLTASGPRPNPQGSYHYGLVNTSRTIILESSAGLINSKQRYAVNRVSFIPADTPLKLADFFKIGGVFRVGSISDRPTGGGMYLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFVVGMDGGQWSESSRGQYNLRDAIARCTIQVYPLSWTAIYMSLDNVGMWNMRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRASGRHTRPL
Homology
BLAST of CmoCh01G020580 vs. ExPASy Swiss-Prot
Match: P29162 (L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 2.3e-166
Identity = 286/574 (49.83%), Postives = 372/574 (64.81%), Query Frame = 0

Query: 11  LSAFLCFFAAVSADDPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDIHCVTNDNLII 70
           ++  LC    V A+DPY +F WNV+YG I PLG+ QQGILINGQFPGP I+C +N+N+++
Sbjct: 9   VALLLCLSVGVIAEDPYLYFNWNVTYGTIAPLGVPQQGILINGQFPGPRINCTSNNNIVV 68

Query: 71  NVFNSLDEPFLISWSLFLLSISLVFILLLTLFSCPPTPTPTPTPTPTLQRRNGVQQRKNS 130
           NVFN+LDEPFL +W                                     NGVQ RKNS
Sbjct: 69  NVFNNLDEPFLFTW-------------------------------------NGVQHRKNS 128

Query: 131 YEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIRILSRPLIPVP 190
           ++DG  GT CPI PG+NFTY  QVKDQIGS+ YFP+ A H+AAGG+GA+ + SR LIPVP
Sbjct: 129 WQDGTPGTMCPIMPGQNFTYRFQVKDQIGSYSYFPTTALHRAAGGYGALNVHSRALIPVP 188

Query: 191 FPDPAGDYSLLIGDWYSSNHTTLKACLDLGKKLPFPDGILINGR------ANYTSFSVEQ 250
           F +PA +Y++ +GDWY+  H TLK  LD G+ +  PDGI+ING+      A    F++E 
Sbjct: 189 FDNPADEYNVFVGDWYNKGHKTLKKILDGGRTIGRPDGIIINGKSAKVGEAKEPLFTMEA 248

Query: 251 GKTYRLRISNVGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSVLVTADQ 310
           GKTYR R  N+G++ S+N R QGH MKLVE+EG+HT+Q  Y SLD+HVGQ  SVLVTADQ
Sbjct: 249 GKTYRYRFCNLGMRSSVNIRFQGHPMKLVELEGSHTVQNIYDSLDLHVGQCLSVLVTADQ 308

Query: 311 PAQDYYIVVSSRFTNPVLTTTGTLRYSNSTGPVKGPLPGAP---TTEVGWSLNQARSIRT 370
             +DYY+VVSSRF    L++   +RY+N  GP    LP  P   T  + WS+NQ RS R 
Sbjct: 309 EPKDYYLVVSSRFLKQALSSVAIIRYANGKGPASPELPTPPPENTEGIAWSMNQFRSFRW 368

Query: 371 NLTASGPRPNPQGSYHYGLVNTSRTIILESSAGLINSKQRYAVNRVSFIPADTPLKLADF 430
           NLTAS  RPNPQGSYHYG +N +RTI + +S   +  K RY +N +S    +TPLKL ++
Sbjct: 369 NLTASAARPNPQGSYHYGQINITRTIKIFNSMSQVGGKLRYGLNGISHTNGETPLKLVEY 428

Query: 431 F-KIGGVFRVGSISDRPTG--GGMYLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSF 490
           F      F+   ++D        + + T+V  A YR FVEI+F+N+E  ++++H+DGYSF
Sbjct: 429 FGATNKAFKYDLMADEAPADPSKLTIATNVKNATYRNFVEIIFENHEKTIRTYHLDGYSF 488

Query: 491 FVVGMDGGQWSESSRGQYNLRDAIARCTIQVYPLSWTAIYMSLDNVGMWNMRSEFWARQY 550
           F V ++ G+WS   R  YNL D ++R  IQVYP SW AI ++ DN GMWN+RSE W + Y
Sbjct: 489 FAVAVEPGRWSPEKRKNYNLVDGLSRNNIQVYPNSWAAIMLTFDNAGMWNLRSEMWEKTY 545

Query: 551 LGQQFYLRVYTPSTSLRDEYPIPKNALLCGRASG 573
           LG+Q Y  V +PS SLRDEY IP N  LCG   G
Sbjct: 549 LGEQLYFSVLSPSRSLRDEYNIPDNHPLCGIVKG 545

BLAST of CmoCh01G020580 vs. ExPASy Swiss-Prot
Match: Q00624 (L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 8.1e-164
Identity = 283/558 (50.72%), Postives = 372/558 (66.67%), Query Frame = 0

Query: 21  VSADDPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDIHCVTNDNLIINVFNSLDEPF 80
           V A+DPY    WNV+YG   PLG+ QQ ILINGQFPGP+I+  +N+N+IINVFN+LDEPF
Sbjct: 21  VHAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNINSTSNNNVIINVFNNLDEPF 80

Query: 81  LISWSLFLLSISLVFILLLTLFSCPPTPTPTPTPTPTLQRRNGVQQRKNSYEDGVYGTTC 140
           L++W                                     NG+Q RKN ++DG  GT C
Sbjct: 81  LLTW-------------------------------------NGIQHRKNCWQDGTPGTMC 140

Query: 141 PIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIRILSRPLIPVPFPDPAGDYSL 200
           PI PG N+TY  Q KDQIGS++Y+P+   H+AAGG+G +R+ SR LIPVP+ DP  DY++
Sbjct: 141 PIMPGTNYTYHFQPKDQIGSYFYYPTTGMHRAAGGYGGLRVNSRLLIPVPYADPEDDYTV 200

Query: 201 LIGDWYSSNHTTLKACLDLGKKLPFPDGILINGR------ANYTSFSVEQGKTYRLRISN 260
           LIGDWY+ +HT LK  LD G+ +  PDGI+ING+      ++   F+++ GKTYR+RI N
Sbjct: 201 LIGDWYTKSHTQLKKFLDGGRTIGRPDGIVINGKSGKGDGSDAPLFTLKPGKTYRVRICN 260

Query: 261 VGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSVLVTADQPAQDYYIVVS 320
           VG++ S+NFRIQ H+MKLVE+EG+H LQ  Y SLD+HVGQ +  +VTA+Q  +DYY+V S
Sbjct: 261 VGVKTSINFRIQNHKMKLVEMEGSHVLQNDYDSLDVHVGQCFGTIVTANQEPKDYYMVAS 320

Query: 321 SRFTNPVLTTTGTLRYSNSTGPVKGPLPGAPTTEVGWSLNQARSIRTNLTASGPRPNPQG 380
           SRF   V+TTTG LRY    GP    LP  P     WSLNQ RS R NLTAS  RPNPQG
Sbjct: 321 SRFLKTVITTTGLLRYEGGKGPASSQLPAGP-VGWAWSLNQFRSFRWNLTASAARPNPQG 380

Query: 381 SYHYGLVNTSRTIILESSAGLINSKQRYAVNRVSFIPADTPLKLADFFKIGG-VFRVGSI 440
           SYHYG +N +RTI L ++ G ++ K R+A+N VS    +TPLKLA++F I   VF+  +I
Sbjct: 381 SYHYGKINITRTIKLVNTQGKVDGKLRFALNGVSHTEPETPLKLAEYFGISDKVFKYDTI 440

Query: 441 SDRPTG---GGMYLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFVVGMDGGQWSE 500
           +D PT      + ++ +V+   +R FVE+VF+N+E  VQSWH+DGYSFF V ++ G W+ 
Sbjct: 441 TDDPTPEQIKNIKIEPNVLNITHRTFVEVVFENHEKSVQSWHLDGYSFFSVAVEPGTWTP 500

Query: 501 SSRGQYNLRDAIARCTIQVYPLSWTAIYMSLDNVGMWNMRSEFWARQYLGQQFYLRVYTP 560
             R  YNL DA++R T+QVYP  W AI ++ DN GMWN+RSE   R+YLGQQ Y  V +P
Sbjct: 501 EKRKNYNLLDAVSRHTVQVYPKCWAAILLTFDNCGMWNVRSENTERRYLGQQLYASVLSP 540

Query: 561 STSLRDEYPIPKNALLCG 569
             SLRDEY +P+ +L CG
Sbjct: 561 EKSLRDEYNMPETSLQCG 540

BLAST of CmoCh01G020580 vs. ExPASy Swiss-Prot
Match: Q8VXX5 (Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=2 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 6.0e-151
Identity = 279/583 (47.86%), Postives = 366/583 (62.78%), Query Frame = 0

Query: 11  LSAFLCFFAAVS----ADDPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDIHCVTND 70
           L++FL  FA +S    A DP+  + + VSY    PLG+ QQ I +NGQFPGP ++  TN 
Sbjct: 8   LASFLLCFALLSAVSFAADPFVSYDFRVSYLTASPLGVPQQVIAVNGQFPGPLLNATTNY 67

Query: 71  NLIINVFNSLDEPFLISWSLFLLSISLVFILLLTLFSCPPTPTPTPTPTPTLQRRNGVQQ 130
           N+++NVFN LDEP L++W                                      G+Q 
Sbjct: 68  NVVVNVFNHLDEPLLLTWP-------------------------------------GIQM 127

Query: 131 RKNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIRILSRPL 190
           R+NS++DGV GT CPIPP  NFTY  QVKDQIGSF+Y PSL F +A+GGFG I I +R +
Sbjct: 128 RRNSWQDGVLGTNCPIPPRWNFTYQFQVKDQIGSFFYSPSLNFQRASGGFGPIVINNRDI 187

Query: 191 IPVPFPDPAGDYSLLIGDWYSSNHTTLKACLDLGKKLPFPDGILINGRA----------- 250
           IP+PFP P G+   +IGDWY+ +H  L+  LD GK+L  PDG+LING+            
Sbjct: 188 IPIPFPQPDGELIFIIGDWYTQDHKALRRALDSGKELGMPDGVLINGKGPYKYNSSVPDG 247

Query: 251 -NYTSFSVEQGKTYRLRISNVGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQ 310
            +Y +F VE GKTYR+R+ NVG+  SLNFRIQ H + LVE EG +T Q  ++  D+HVGQ
Sbjct: 248 IDYLTFHVEPGKTYRIRVHNVGISTSLNFRIQNHSLLLVETEGHYTSQANFTDFDVHVGQ 307

Query: 311 SYSVLVTADQPA-QDYYIVVSSRFTNPV----LTTTGTLRYSNSTGPVKGPLPGAPTTEV 370
           SYS LVT DQ A  DYYIV S+RF N      +T    L YSNS GPV GPLP  P T+V
Sbjct: 308 SYSFLVTMDQDATSDYYIVASARFVNETVWQRVTGVAILHYSNSKGPVSGPLP-VPKTDV 367

Query: 371 G--WS-LNQARSIRTNLTASGPRPNPQGSYHYGLVNTSRTIILES-SAGLINSKQRYAVN 430
              WS ++Q ++IR N +ASG RPNPQGS+HYG +N + T IL S    +IN   R  +N
Sbjct: 368 SSPWSAMSQPKTIRQNTSASGARPNPQGSFHYGQINITNTYILRSLPPTIINGALRATLN 427

Query: 431 RVSFIPADTPLKLADFFKIGGVFRVGSISDRPTGGGMYLDTSVMGADYRAFVEIVFQNNE 490
            +SF+   TP++LAD  K+ G +++    DRP    + LD S++ A Y+ F+++VFQNN+
Sbjct: 428 GISFVNPSTPVRLADRNKVKGAYKL-DFPDRPFNRPLRLDRSMINATYKGFIQVVFQNND 487

Query: 491 NIVQSWHIDGYSFFVVGMDGGQWSESSRGQYNLRDAIARCTIQVYPLSWTAIYMSLDNVG 550
             +QS+H+DGYSFFVVGMD G WSE  +G YN  DAI+R TI+VYP  WTA+ +SLDNVG
Sbjct: 488 TKIQSFHVDGYSFFVVGMDFGIWSEDKKGSYNNWDAISRSTIEVYPGGWTAVLISLDNVG 547

Query: 551 MWNMRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCG 569
           +WN+R E   R YLG++ Y+R+  P    + E   P N L CG
Sbjct: 548 VWNIRVENLDRWYLGEETYMRITNPEEDGKTEMDPPDNVLYCG 551

BLAST of CmoCh01G020580 vs. ExPASy Swiss-Prot
Match: Q9SU40 (Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=1 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 7.4e-149
Identity = 280/581 (48.19%), Postives = 362/581 (62.31%), Query Frame = 0

Query: 15  LCFFAAVS---ADDPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDIHCVTNDNLIIN 74
           L FF  +S   A DPY F+ + VSY    PLG+ QQ I ING+FPGP I+  TN+NL++N
Sbjct: 9   LVFFVNISFCFAADPYSFYNFEVSYITASPLGVPQQVIAINGKFPGPTINVTTNENLVVN 68

Query: 75  VFNSLDEPFLISWSLFLLSISLVFILLLTLFSCPPTPTPTPTPTPTLQRRNGVQQRKNSY 134
           V N LDE  L+ W                                     NG+QQR+ S+
Sbjct: 69  VRNKLDEGLLLHW-------------------------------------NGIQQRRVSW 128

Query: 135 EDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIRILSRPLIPVPF 194
           +DGV GT CPIPP  N+TY  QVKDQIGSF+YFPSL F +A+GGFG+  +  R +IPVPF
Sbjct: 129 QDGVLGTNCPIPPKWNWTYEFQVKDQIGSFFYFPSLHFQRASGGFGSFVVNPRAIIPVPF 188

Query: 195 PDPAGDYSLLIGDWYSSNHTTLKACLDLGKKLPFPDGILINGRANY-------------T 254
             P GD ++ IGDWY  NHT L+  LD GK L  PDG+LING+  Y              
Sbjct: 189 STPDGDITVTIGDWYIRNHTALRKALDDGKDLGMPDGVLINGKGPYRYNDTLVADGIDFE 248

Query: 255 SFSVEQGKTYRLRISNVGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSV 314
           + +V  GKTYRLR+SNVG+  SLNFRIQGH + L E EG++T+Q  Y+SLDIHVGQSYS 
Sbjct: 249 TITVHPGKTYRLRVSNVGISTSLNFRIQGHNLVLAESEGSYTVQQNYTSLDIHVGQSYSF 308

Query: 315 LVTADQPA-QDYYIVVSSRFTNPVL----TTTGTLRYSNSTGPVKGPLPGAPTTEVG--W 374
           LVT DQ A  DYYIV S+R  N  +    T  G L+Y+NS G  KG LP  P  E    +
Sbjct: 309 LVTMDQNASSDYYIVASARVVNETIWRRVTGVGILKYTNSKGKAKGQLPPGPQDEFDKTF 368

Query: 375 SLNQARSIRTNLTASGPRPNPQGSYHYGLVNTSRTIILESSAGL-INSKQRYAVNRVSFI 434
           S+NQARSIR N++ASG RPNPQGS+ YG +N +   +L +   + I+ K+R  +N +SF 
Sbjct: 369 SMNQARSIRWNVSASGARPNPQGSFKYGSINVTDVYVLRNMPPVTISGKRRTTLNGISFK 428

Query: 435 PADTPLKLADFFKIGGVFRVGSISDRPTGGGMYLDTSVMGADYRAFVEIVFQNNENIVQS 494
              TP++LAD  K+  V+++     RP  G   + TS++   YR F+E+V QNN+  +QS
Sbjct: 429 NPSTPIRLADKLKVKDVYKL-DFPKRPLTGPAKVATSIINGTYRGFMEVVLQNNDTKMQS 488

Query: 495 WHIDGYSFFVVGMDGGQWSESSRGQYNLRDAIARCTIQVYPLSWTAIYMSLDNVGMWNMR 554
           +H+ GY+FFVVGMD G+W+E+SRG YN  D IAR TIQVYP +W+AI +SLDN G WN+R
Sbjct: 489 YHMSGYAFFVVGMDYGEWTENSRGTYNKWDGIARSTIQVYPGAWSAILISLDNPGAWNLR 548

Query: 555 SEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCGRAS 572
           +E     YLGQ+ Y+RV  P  + + E+  P N L CG  S
Sbjct: 549 TENLDSWYLGQETYVRVVNPDENNKTEFGHPDNVLYCGALS 551

BLAST of CmoCh01G020580 vs. ExPASy Swiss-Prot
Match: Q9FHN6 (Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=2 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 1.9e-144
Identity = 268/582 (46.05%), Postives = 358/582 (61.51%), Query Frame = 0

Query: 8   AFALSAFLCFFAAVSADDPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDIHCVTNDN 67
           AF  S F   F    A DPY  + + +SY    PLG+ QQ I +NG+FPGP I+  TN N
Sbjct: 9   AFVFS-FALIFGFSFAGDPYVSYDFTLSYITASPLGVPQQVIAVNGKFPGPVINATTNYN 68

Query: 68  LIINVFNSLDEPFLISWSLFLLSISLVFILLLTLFSCPPTPTPTPTPTPTLQRRNGVQQR 127
           + +NV N LDEP L++W                                      GVQ R
Sbjct: 69  VHVNVLNHLDEPLLLTWP-------------------------------------GVQMR 128

Query: 128 KNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIRILSRPLI 187
           +NS++DGV GT CPIPP  NFTY  Q+KDQIGS++Y PSL F +A+GGFGA+ I +R L+
Sbjct: 129 RNSWQDGVLGTNCPIPPNWNFTYDFQLKDQIGSYFYSPSLNFQRASGGFGALIINNRDLV 188

Query: 188 PVPFPDPAGDYSLLIGDWYSSNHTTLKACLDLGKKLPFPDGILINGRA------------ 247
           P+PF +P G+   +IGDWY+ NHT L+  LD GK+L  PDG+LING+             
Sbjct: 189 PIPFTEPDGEIIFIIGDWYTQNHTALRRILDSGKELGMPDGVLINGKGPFKYNSSVPDGI 248

Query: 248 NYTSFSVEQGKTYRLRISNVGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQS 307
            + + +V+ GKTYR+R+ NVG+  SLNFRIQ H++ L+E EG +T Q  ++  D+HVGQS
Sbjct: 249 EHETVNVDPGKTYRIRVHNVGISTSLNFRIQNHKLLLIETEGRYTSQMNFTDFDVHVGQS 308

Query: 308 YSVLVTADQPA-QDYYIVVSSRFTNPV----LTTTGTLRYSNSTGPVKGPLP-GAPTTEV 367
           YS LVT DQ A  DYYIV S+RF N      +T  G L YSNS GP  GPLP  A     
Sbjct: 309 YSFLVTMDQNATSDYYIVASARFVNETVWQRVTGVGILHYSNSKGPASGPLPVSATDVNH 368

Query: 368 GWS-LNQARSIRTNLTASGPRPNPQGSYHYGLVNTSRTIILES-SAGLINSKQRYAVNRV 427
            WS +NQ R+I+ N +ASG RPNPQGS+HYG +N +RT IL S     IN K R  +N +
Sbjct: 369 PWSAMNQPRAIKQNTSASGARPNPQGSFHYGQINITRTYILRSLPPTKINGKLRATLNGI 428

Query: 428 SFIPADTPLKLADFFKIGGVFRVGSISDRPTGGGM-YLDTSVMGADYRAFVEIVFQNNEN 487
           SF+   TP++LAD  K+ G + +    DRP    +  L +S++ A Y+ F++++FQNN+ 
Sbjct: 429 SFVNPSTPMRLADDHKVKGDYML-DFPDRPLDEKLPRLSSSIINATYKGFIQVIFQNNDT 488

Query: 488 IVQSWHIDGYSFFVVGMDGGQWSESSRGQYNLRDAIARCTIQVYPLSWTAIYMSLDNVGM 547
            +QS+HIDGY+F+VV MD G WSE     YN  DA+AR T++VYP +WTA+ +SLDNVG+
Sbjct: 489 KIQSFHIDGYAFYVVAMDFGIWSEDRNSSYNNWDAVARSTVEVYPGAWTAVLISLDNVGV 548

Query: 548 WNMRSEFWARQYLGQQFYLRVYTPSTSLRDEYPIPKNALLCG 569
           WN+R E   R YLGQ+ Y+R+  P  +   E   P+N + CG
Sbjct: 549 WNIRVENLDRWYLGQETYMRIINPEENGSTEMDPPENVMYCG 551

BLAST of CmoCh01G020580 vs. ExPASy TrEMBL
Match: A0A6J1FJ99 (L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111445880 PE=3 SV=1)

HSP 1 Score: 1100.5 bits (2845), Expect = 0.0e+00
Identity = 541/578 (93.60%), Postives = 541/578 (93.60%), Query Frame = 0

Query: 1   MLLLTSPAFALSAFLCFFAAVSADDPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDI 60
           MLLLTSPAFALSAFLCFFAAVSADDPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDI
Sbjct: 1   MLLLTSPAFALSAFLCFFAAVSADDPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDI 60

Query: 61  HCVTNDNLIINVFNSLDEPFLISWSLFLLSISLVFILLLTLFSCPPTPTPTPTPTPTLQR 120
           HCVTNDNLIINVFNSLDEPFLISW                                    
Sbjct: 61  HCVTNDNLIINVFNSLDEPFLISW------------------------------------ 120

Query: 121 RNGVQQRKNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIR 180
            NGVQQRKNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIR
Sbjct: 121 -NGVQQRKNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIR 180

Query: 181 ILSRPLIPVPFPDPAGDYSLLIGDWYSSNHTTLKACLDLGKKLPFPDGILINGRANYTSF 240
           ILSRPLIPVPFPDPAGDYSLLIGDWYSSNHTTLKACLDLGKKLPFPDGILINGRANYTSF
Sbjct: 181 ILSRPLIPVPFPDPAGDYSLLIGDWYSSNHTTLKACLDLGKKLPFPDGILINGRANYTSF 240

Query: 241 SVEQGKTYRLRISNVGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSVLV 300
           SVEQGKTYRLRISNVGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSVLV
Sbjct: 241 SVEQGKTYRLRISNVGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSVLV 300

Query: 301 TADQPAQDYYIVVSSRFTNPVLTTTGTLRYSNSTGPVKGPLPGAPTTEVGWSLNQARSIR 360
           TADQPAQDYYIVVSSRFTNPVLTTTGTLRYSNSTGPVKGPLPGAPTTEVGWSLNQARSIR
Sbjct: 301 TADQPAQDYYIVVSSRFTNPVLTTTGTLRYSNSTGPVKGPLPGAPTTEVGWSLNQARSIR 360

Query: 361 TNLTASGPRPNPQGSYHYGLVNTSRTIILESSAGLINSKQRYAVNRVSFIPADTPLKLAD 420
           TNLTASGPRPNPQGSYHYGLVNTSRTIILESSAGLINSKQRYAVNRVSFIPADTPLKLAD
Sbjct: 361 TNLTASGPRPNPQGSYHYGLVNTSRTIILESSAGLINSKQRYAVNRVSFIPADTPLKLAD 420

Query: 421 FFKIGGVFRVGSISDRPTGGGMYLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFV 480
           FFKIGGVFRVGSISDRPTGGGMYLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFV
Sbjct: 421 FFKIGGVFRVGSISDRPTGGGMYLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFV 480

Query: 481 VGMDGGQWSESSRGQYNLRDAIARCTIQVYPLSWTAIYMSLDNVGMWNMRSEFWARQYLG 540
           VGMDGGQWSESSRGQYNLRDAIARCTIQVYPLSWTAIYMSLDNVGMWNMRSEFWARQYLG
Sbjct: 481 VGMDGGQWSESSRGQYNLRDAIARCTIQVYPLSWTAIYMSLDNVGMWNMRSEFWARQYLG 540

Query: 541 QQFYLRVYTPSTSLRDEYPIPKNALLCGRASGRHTRPL 579
           QQFYLRVYTPSTSLRDEYPIPKNALLCGRASGRHTRPL
Sbjct: 541 QQFYLRVYTPSTSLRDEYPIPKNALLCGRASGRHTRPL 541

BLAST of CmoCh01G020580 vs. ExPASy TrEMBL
Match: A0A6J1J469 (L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111481188 PE=3 SV=1)

HSP 1 Score: 1070.1 bits (2766), Expect = 3.2e-309
Identity = 528/578 (91.35%), Postives = 534/578 (92.39%), Query Frame = 0

Query: 1   MLLLTSPAFALSAFLCFFAAVSADDPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDI 60
           MLLLTSPA ALSAFLCFFAAVSA++PYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDI
Sbjct: 1   MLLLTSPALALSAFLCFFAAVSAENPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDI 60

Query: 61  HCVTNDNLIINVFNSLDEPFLISWSLFLLSISLVFILLLTLFSCPPTPTPTPTPTPTLQR 120
           +CVTNDNLIINVFNSLDEPFLISW                                    
Sbjct: 61  YCVTNDNLIINVFNSLDEPFLISW------------------------------------ 120

Query: 121 RNGVQQRKNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIR 180
            NGVQQRKNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIR
Sbjct: 121 -NGVQQRKNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIR 180

Query: 181 ILSRPLIPVPFPDPAGDYSLLIGDWYSSNHTTLKACLDLGKKLPFPDGILINGRANYTSF 240
           ILSRPLIPVPFPDPAGDY+LL+GDWYSSNHTTLKA LDLGKKLPFPDGILING AN TSF
Sbjct: 181 ILSRPLIPVPFPDPAGDYTLLVGDWYSSNHTTLKARLDLGKKLPFPDGILINGGANRTSF 240

Query: 241 SVEQGKTYRLRISNVGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSVLV 300
            VEQGKTYRLRISNVGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSVLV
Sbjct: 241 IVEQGKTYRLRISNVGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSVLV 300

Query: 301 TADQPAQDYYIVVSSRFTNPVLTTTGTLRYSNSTGPVKGPLPGAPTTEVGWSLNQARSIR 360
           TADQPAQDYYIVVSSRFTNPVLTTTGTLRYSNSTGPVKGPLPGAPT EVGWSLNQARSIR
Sbjct: 301 TADQPAQDYYIVVSSRFTNPVLTTTGTLRYSNSTGPVKGPLPGAPTIEVGWSLNQARSIR 360

Query: 361 TNLTASGPRPNPQGSYHYGLVNTSRTIILESSAGLINSKQRYAVNRVSFIPADTPLKLAD 420
           TNLTASGPRPNPQGSYHYGLVNTSRTIILESSAGLINSKQRYAVNRVSFIPADTPLKLAD
Sbjct: 361 TNLTASGPRPNPQGSYHYGLVNTSRTIILESSAGLINSKQRYAVNRVSFIPADTPLKLAD 420

Query: 421 FFKIGGVFRVGSISDRPTGGGMYLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFV 480
           FFKIGGVFRVGSISDRPTGGGMYLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFV
Sbjct: 421 FFKIGGVFRVGSISDRPTGGGMYLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFV 480

Query: 481 VGMDGGQWSESSRGQYNLRDAIARCTIQVYPLSWTAIYMSLDNVGMWNMRSEFWARQYLG 540
           VGMDGGQWSESSRGQYNLRDAIAR TIQVYPLSWTAIYMSLDNVGMWN+RSEFWARQYLG
Sbjct: 481 VGMDGGQWSESSRGQYNLRDAIARSTIQVYPLSWTAIYMSLDNVGMWNIRSEFWARQYLG 540

Query: 541 QQFYLRVYTPSTSLRDEYPIPKNALLCGRASGRHTRPL 579
           QQFYLRVYTPSTSLRDEYPIPKNALLCGRASGRHTRPL
Sbjct: 541 QQFYLRVYTPSTSLRDEYPIPKNALLCGRASGRHTRPL 541

BLAST of CmoCh01G020580 vs. ExPASy TrEMBL
Match: A0A5A7VBL1 (L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00820 PE=3 SV=1)

HSP 1 Score: 1003.4 bits (2593), Expect = 3.8e-289
Identity = 484/578 (83.74%), Postives = 514/578 (88.93%), Query Frame = 0

Query: 1   MLLLTSPAFALSAFLCFFAAVSADDPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDI 60
           ML LTSP FAL AF CFF+ +SA++PYRFFTWNVSY +IYPLG+RQQGILINGQFPGPDI
Sbjct: 1   MLPLTSPFFALCAFFCFFSTLSAENPYRFFTWNVSYANIYPLGVRQQGILINGQFPGPDI 60

Query: 61  HCVTNDNLIINVFNSLDEPFLISWSLFLLSISLVFILLLTLFSCPPTPTPTPTPTPTLQR 120
           HCVTNDNLIINVFNSLDEPFLISW                                    
Sbjct: 61  HCVTNDNLIINVFNSLDEPFLISW------------------------------------ 120

Query: 121 RNGVQQRKNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIR 180
            NG+QQR+NSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFG IR
Sbjct: 121 -NGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGGIR 180

Query: 181 ILSRPLIPVPFPDPAGDYSLLIGDWYSSNHTTLKACLDLGKKLPFPDGILINGRANYTSF 240
           ILSRP IPVPFPDPAGDY++LIGDWY SNHTTLKA LD GKKLPFPDGILINGRAN TSF
Sbjct: 181 ILSRPRIPVPFPDPAGDYTVLIGDWYKSNHTTLKAHLDRGKKLPFPDGILINGRANDTSF 240

Query: 241 SVEQGKTYRLRISNVGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSVLV 300
           SVEQGKTYRLRISNVGLQHSLNFRIQGH+MKLVEVEGTHTLQTTYSSLD+HVGQSYSVLV
Sbjct: 241 SVEQGKTYRLRISNVGLQHSLNFRIQGHKMKLVEVEGTHTLQTTYSSLDVHVGQSYSVLV 300

Query: 301 TADQPAQDYYIVVSSRFTNPVLTTTGTLRYSNSTGPVKGPLPGAPTTEVGWSLNQARSIR 360
           TADQPAQDYYIVVS+RFT+ VL TTG LRYSNS GPVKGP PG PT ++ WSLNQARSIR
Sbjct: 301 TADQPAQDYYIVVSTRFTSRVLATTGILRYSNSAGPVKGPPPGGPTIQIDWSLNQARSIR 360

Query: 361 TNLTASGPRPNPQGSYHYGLVNTSRTIILESSAGLINSKQRYAVNRVSFIPADTPLKLAD 420
           TNLTASGPRPNPQGSYHYGL+NT++TIIL +SAG +N KQRYAVN VSF+PADTPLKLAD
Sbjct: 361 TNLTASGPRPNPQGSYHYGLINTTKTIILANSAGQVNRKQRYAVNSVSFVPADTPLKLAD 420

Query: 421 FFKIGGVFRVGSISDRPTGGGMYLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFV 480
           FFKIGGVFRVGSISDRPTGGG+YLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFV
Sbjct: 421 FFKIGGVFRVGSISDRPTGGGIYLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFV 480

Query: 481 VGMDGGQWSESSRGQYNLRDAIARCTIQVYPLSWTAIYMSLDNVGMWNMRSEFWARQYLG 540
           VGMDGGQW++SSR QYNLRDAIARCT QVYP SWTAIY++LDNVG+WN+RSEFWARQYLG
Sbjct: 481 VGMDGGQWTQSSRNQYNLRDAIARCTTQVYPYSWTAIYVALDNVGLWNVRSEFWARQYLG 540

Query: 541 QQFYLRVYTPSTSLRDEYPIPKNALLCGRASGRHTRPL 579
           QQFYLRVYTPSTSLRDE+PIPKNALLCGRASGRHTRPL
Sbjct: 541 QQFYLRVYTPSTSLRDEFPIPKNALLCGRASGRHTRPL 541

BLAST of CmoCh01G020580 vs. ExPASy TrEMBL
Match: E5GCD6 (Multicopper oxidase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 1003.4 bits (2593), Expect = 3.8e-289
Identity = 484/578 (83.74%), Postives = 514/578 (88.93%), Query Frame = 0

Query: 1   MLLLTSPAFALSAFLCFFAAVSADDPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDI 60
           ML LTSP FAL AF CFF+ +SA++PYRFFTWNVSY +IYPLG+RQQGILINGQFPGPDI
Sbjct: 1   MLPLTSPFFALCAFFCFFSTLSAENPYRFFTWNVSYANIYPLGVRQQGILINGQFPGPDI 60

Query: 61  HCVTNDNLIINVFNSLDEPFLISWSLFLLSISLVFILLLTLFSCPPTPTPTPTPTPTLQR 120
           HCVTNDNLIINVFNSLDEPFLISW                                    
Sbjct: 61  HCVTNDNLIINVFNSLDEPFLISW------------------------------------ 120

Query: 121 RNGVQQRKNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIR 180
            NG+QQR+NSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFG IR
Sbjct: 121 -NGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGGIR 180

Query: 181 ILSRPLIPVPFPDPAGDYSLLIGDWYSSNHTTLKACLDLGKKLPFPDGILINGRANYTSF 240
           ILSRP IPVPFPDPAGDY++LIGDWY SNHTTLKA LD GKKLPFPDGILINGRAN TSF
Sbjct: 181 ILSRPRIPVPFPDPAGDYTVLIGDWYKSNHTTLKAHLDRGKKLPFPDGILINGRANDTSF 240

Query: 241 SVEQGKTYRLRISNVGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSVLV 300
           SVEQGKTYRLRISNVGLQHSLNFRIQGH+MKLVEVEGTHTLQTTYSSLD+HVGQSYSVLV
Sbjct: 241 SVEQGKTYRLRISNVGLQHSLNFRIQGHKMKLVEVEGTHTLQTTYSSLDVHVGQSYSVLV 300

Query: 301 TADQPAQDYYIVVSSRFTNPVLTTTGTLRYSNSTGPVKGPLPGAPTTEVGWSLNQARSIR 360
           TADQPAQDYYIVVS+RFT+ VL TTG LRYSNS GPVKGP PG PT ++ WSLNQARSIR
Sbjct: 301 TADQPAQDYYIVVSTRFTSRVLATTGILRYSNSAGPVKGPPPGGPTIQIDWSLNQARSIR 360

Query: 361 TNLTASGPRPNPQGSYHYGLVNTSRTIILESSAGLINSKQRYAVNRVSFIPADTPLKLAD 420
           TNLTASGPRPNPQGSYHYGL+NT++TIIL +SAG +N KQRYAVN VSF+PADTPLKLAD
Sbjct: 361 TNLTASGPRPNPQGSYHYGLINTTKTIILANSAGQVNRKQRYAVNSVSFVPADTPLKLAD 420

Query: 421 FFKIGGVFRVGSISDRPTGGGMYLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFV 480
           FFKIGGVFRVGSISDRPTGGG+YLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFV
Sbjct: 421 FFKIGGVFRVGSISDRPTGGGIYLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFV 480

Query: 481 VGMDGGQWSESSRGQYNLRDAIARCTIQVYPLSWTAIYMSLDNVGMWNMRSEFWARQYLG 540
           VGMDGGQW++SSR QYNLRDAIARCT QVYP SWTAIY++LDNVG+WN+RSEFWARQYLG
Sbjct: 481 VGMDGGQWTQSSRNQYNLRDAIARCTTQVYPYSWTAIYVALDNVGLWNVRSEFWARQYLG 540

Query: 541 QQFYLRVYTPSTSLRDEYPIPKNALLCGRASGRHTRPL 579
           QQFYLRVYTPSTSLRDE+PIPKNALLCGRASGRHTRPL
Sbjct: 541 QQFYLRVYTPSTSLRDEFPIPKNALLCGRASGRHTRPL 541

BLAST of CmoCh01G020580 vs. ExPASy TrEMBL
Match: A0A1S3BUY9 (L-ascorbate oxidase homolog OS=Cucumis melo OX=3656 GN=LOC103493793 PE=3 SV=1)

HSP 1 Score: 1003.4 bits (2593), Expect = 3.8e-289
Identity = 484/578 (83.74%), Postives = 514/578 (88.93%), Query Frame = 0

Query: 1   MLLLTSPAFALSAFLCFFAAVSADDPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDI 60
           ML LTSP FAL AF CFF+ +SA++PYRFFTWNVSY +IYPLG+RQQGILINGQFPGPDI
Sbjct: 1   MLPLTSPFFALCAFFCFFSTLSAENPYRFFTWNVSYANIYPLGVRQQGILINGQFPGPDI 60

Query: 61  HCVTNDNLIINVFNSLDEPFLISWSLFLLSISLVFILLLTLFSCPPTPTPTPTPTPTLQR 120
           HCVTNDNLIINVFNSLDEPFLISW                                    
Sbjct: 61  HCVTNDNLIINVFNSLDEPFLISW------------------------------------ 120

Query: 121 RNGVQQRKNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIR 180
            NG+QQR+NSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFG IR
Sbjct: 121 -NGIQQRRNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGGIR 180

Query: 181 ILSRPLIPVPFPDPAGDYSLLIGDWYSSNHTTLKACLDLGKKLPFPDGILINGRANYTSF 240
           ILSRP IPVPFPDPAGDY++LIGDWY SNHTTLKA LD GKKLPFPDGILINGRAN TSF
Sbjct: 181 ILSRPRIPVPFPDPAGDYTVLIGDWYKSNHTTLKAHLDRGKKLPFPDGILINGRANDTSF 240

Query: 241 SVEQGKTYRLRISNVGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSVLV 300
           SVEQGKTYRLRISNVGLQHSLNFRIQGH+MKLVEVEGTHTLQTTYSSLD+HVGQSYSVLV
Sbjct: 241 SVEQGKTYRLRISNVGLQHSLNFRIQGHKMKLVEVEGTHTLQTTYSSLDVHVGQSYSVLV 300

Query: 301 TADQPAQDYYIVVSSRFTNPVLTTTGTLRYSNSTGPVKGPLPGAPTTEVGWSLNQARSIR 360
           TADQPAQDYYIVVS+RFT+ VL TTG LRYSNS GPVKGP PG PT ++ WSLNQARSIR
Sbjct: 301 TADQPAQDYYIVVSTRFTSRVLATTGILRYSNSAGPVKGPPPGGPTIQIDWSLNQARSIR 360

Query: 361 TNLTASGPRPNPQGSYHYGLVNTSRTIILESSAGLINSKQRYAVNRVSFIPADTPLKLAD 420
           TNLTASGPRPNPQGSYHYGL+NT++TIIL +SAG +N KQRYAVN VSF+PADTPLKLAD
Sbjct: 361 TNLTASGPRPNPQGSYHYGLINTTKTIILANSAGQVNRKQRYAVNSVSFVPADTPLKLAD 420

Query: 421 FFKIGGVFRVGSISDRPTGGGMYLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFV 480
           FFKIGGVFRVGSISDRPTGGG+YLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFV
Sbjct: 421 FFKIGGVFRVGSISDRPTGGGIYLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFV 480

Query: 481 VGMDGGQWSESSRGQYNLRDAIARCTIQVYPLSWTAIYMSLDNVGMWNMRSEFWARQYLG 540
           VGMDGGQW++SSR QYNLRDAIARCT QVYP SWTAIY++LDNVG+WN+RSEFWARQYLG
Sbjct: 481 VGMDGGQWTQSSRNQYNLRDAIARCTTQVYPYSWTAIYVALDNVGLWNVRSEFWARQYLG 540

Query: 541 QQFYLRVYTPSTSLRDEYPIPKNALLCGRASGRHTRPL 579
           QQFYLRVYTPSTSLRDE+PIPKNALLCGRASGRHTRPL
Sbjct: 541 QQFYLRVYTPSTSLRDEFPIPKNALLCGRASGRHTRPL 541

BLAST of CmoCh01G020580 vs. NCBI nr
Match: XP_022940174.1 (L-ascorbate oxidase homolog [Cucurbita moschata])

HSP 1 Score: 1100.5 bits (2845), Expect = 0.0e+00
Identity = 541/578 (93.60%), Postives = 541/578 (93.60%), Query Frame = 0

Query: 1   MLLLTSPAFALSAFLCFFAAVSADDPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDI 60
           MLLLTSPAFALSAFLCFFAAVSADDPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDI
Sbjct: 1   MLLLTSPAFALSAFLCFFAAVSADDPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDI 60

Query: 61  HCVTNDNLIINVFNSLDEPFLISWSLFLLSISLVFILLLTLFSCPPTPTPTPTPTPTLQR 120
           HCVTNDNLIINVFNSLDEPFLISW                                    
Sbjct: 61  HCVTNDNLIINVFNSLDEPFLISW------------------------------------ 120

Query: 121 RNGVQQRKNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIR 180
            NGVQQRKNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIR
Sbjct: 121 -NGVQQRKNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIR 180

Query: 181 ILSRPLIPVPFPDPAGDYSLLIGDWYSSNHTTLKACLDLGKKLPFPDGILINGRANYTSF 240
           ILSRPLIPVPFPDPAGDYSLLIGDWYSSNHTTLKACLDLGKKLPFPDGILINGRANYTSF
Sbjct: 181 ILSRPLIPVPFPDPAGDYSLLIGDWYSSNHTTLKACLDLGKKLPFPDGILINGRANYTSF 240

Query: 241 SVEQGKTYRLRISNVGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSVLV 300
           SVEQGKTYRLRISNVGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSVLV
Sbjct: 241 SVEQGKTYRLRISNVGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSVLV 300

Query: 301 TADQPAQDYYIVVSSRFTNPVLTTTGTLRYSNSTGPVKGPLPGAPTTEVGWSLNQARSIR 360
           TADQPAQDYYIVVSSRFTNPVLTTTGTLRYSNSTGPVKGPLPGAPTTEVGWSLNQARSIR
Sbjct: 301 TADQPAQDYYIVVSSRFTNPVLTTTGTLRYSNSTGPVKGPLPGAPTTEVGWSLNQARSIR 360

Query: 361 TNLTASGPRPNPQGSYHYGLVNTSRTIILESSAGLINSKQRYAVNRVSFIPADTPLKLAD 420
           TNLTASGPRPNPQGSYHYGLVNTSRTIILESSAGLINSKQRYAVNRVSFIPADTPLKLAD
Sbjct: 361 TNLTASGPRPNPQGSYHYGLVNTSRTIILESSAGLINSKQRYAVNRVSFIPADTPLKLAD 420

Query: 421 FFKIGGVFRVGSISDRPTGGGMYLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFV 480
           FFKIGGVFRVGSISDRPTGGGMYLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFV
Sbjct: 421 FFKIGGVFRVGSISDRPTGGGMYLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFV 480

Query: 481 VGMDGGQWSESSRGQYNLRDAIARCTIQVYPLSWTAIYMSLDNVGMWNMRSEFWARQYLG 540
           VGMDGGQWSESSRGQYNLRDAIARCTIQVYPLSWTAIYMSLDNVGMWNMRSEFWARQYLG
Sbjct: 481 VGMDGGQWSESSRGQYNLRDAIARCTIQVYPLSWTAIYMSLDNVGMWNMRSEFWARQYLG 540

Query: 541 QQFYLRVYTPSTSLRDEYPIPKNALLCGRASGRHTRPL 579
           QQFYLRVYTPSTSLRDEYPIPKNALLCGRASGRHTRPL
Sbjct: 541 QQFYLRVYTPSTSLRDEYPIPKNALLCGRASGRHTRPL 541

BLAST of CmoCh01G020580 vs. NCBI nr
Match: KAG7037936.1 (hypothetical protein SDJN02_01569, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1095.5 bits (2832), Expect = 0.0e+00
Identity = 538/578 (93.08%), Postives = 541/578 (93.60%), Query Frame = 0

Query: 1   MLLLTSPAFALSAFLCFFAAVSADDPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDI 60
           MLLLTSPAFALSAFLCFFAAVSA+DPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDI
Sbjct: 1   MLLLTSPAFALSAFLCFFAAVSAEDPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDI 60

Query: 61  HCVTNDNLIINVFNSLDEPFLISWSLFLLSISLVFILLLTLFSCPPTPTPTPTPTPTLQR 120
           HCVTNDNLIINVFNSLDEPFLISW                                    
Sbjct: 61  HCVTNDNLIINVFNSLDEPFLISW------------------------------------ 120

Query: 121 RNGVQQRKNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIR 180
            NGVQQRKNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIR
Sbjct: 121 -NGVQQRKNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIR 180

Query: 181 ILSRPLIPVPFPDPAGDYSLLIGDWYSSNHTTLKACLDLGKKLPFPDGILINGRANYTSF 240
           ILSRPLIPVPFPDPAGDYSLLIGDWYSSNHTTLKACLDLGKKLPFPDGILINGRANYTSF
Sbjct: 181 ILSRPLIPVPFPDPAGDYSLLIGDWYSSNHTTLKACLDLGKKLPFPDGILINGRANYTSF 240

Query: 241 SVEQGKTYRLRISNVGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSVLV 300
           SVEQGKTYRLRISNVGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSVLV
Sbjct: 241 SVEQGKTYRLRISNVGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSVLV 300

Query: 301 TADQPAQDYYIVVSSRFTNPVLTTTGTLRYSNSTGPVKGPLPGAPTTEVGWSLNQARSIR 360
           TADQPAQDYYIVVSSRFTNPVLTTTGTLRYSNSTGPVKGPLPGAPTTEVGWSLNQARSIR
Sbjct: 301 TADQPAQDYYIVVSSRFTNPVLTTTGTLRYSNSTGPVKGPLPGAPTTEVGWSLNQARSIR 360

Query: 361 TNLTASGPRPNPQGSYHYGLVNTSRTIILESSAGLINSKQRYAVNRVSFIPADTPLKLAD 420
           TNLTASGPRPNPQGSYHYGLVNTSRTIILESSAGLINSKQRYAVNRVSFIPADTPLKLAD
Sbjct: 361 TNLTASGPRPNPQGSYHYGLVNTSRTIILESSAGLINSKQRYAVNRVSFIPADTPLKLAD 420

Query: 421 FFKIGGVFRVGSISDRPTGGGMYLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFV 480
           FFKIGGVFRVGSISDRPTGGGMYLDTSVMGADYRAFVEIVFQN+ENIVQSWHIDGYSFFV
Sbjct: 421 FFKIGGVFRVGSISDRPTGGGMYLDTSVMGADYRAFVEIVFQNSENIVQSWHIDGYSFFV 480

Query: 481 VGMDGGQWSESSRGQYNLRDAIARCTIQVYPLSWTAIYMSLDNVGMWNMRSEFWARQYLG 540
           VGMDGGQWSESSRGQYNLRDAIARCTIQVYPLSWTAIYMSLDNVGMWN+RSEFWARQYLG
Sbjct: 481 VGMDGGQWSESSRGQYNLRDAIARCTIQVYPLSWTAIYMSLDNVGMWNIRSEFWARQYLG 540

Query: 541 QQFYLRVYTPSTSLRDEYPIPKNALLCGRASGRHTRPL 579
           QQFYLRVYTPSTSLRDEYPIPKNALLCGRASGRHTRPL
Sbjct: 541 QQFYLRVYTPSTSLRDEYPIPKNALLCGRASGRHTRPL 541

BLAST of CmoCh01G020580 vs. NCBI nr
Match: XP_023525181.1 (L-ascorbate oxidase homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1083.6 bits (2801), Expect = 0.0e+00
Identity = 531/578 (91.87%), Postives = 537/578 (92.91%), Query Frame = 0

Query: 1   MLLLTSPAFALSAFLCFFAAVSADDPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDI 60
           MLLLTSPAFALSAFLCFFAAVSA+DPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDI
Sbjct: 1   MLLLTSPAFALSAFLCFFAAVSAEDPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDI 60

Query: 61  HCVTNDNLIINVFNSLDEPFLISWSLFLLSISLVFILLLTLFSCPPTPTPTPTPTPTLQR 120
           +CVTNDNLIINVFNSLDEPFLISW                                    
Sbjct: 61  YCVTNDNLIINVFNSLDEPFLISW------------------------------------ 120

Query: 121 RNGVQQRKNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIR 180
            NGVQQRKNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIR
Sbjct: 121 -NGVQQRKNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIR 180

Query: 181 ILSRPLIPVPFPDPAGDYSLLIGDWYSSNHTTLKACLDLGKKLPFPDGILINGRANYTSF 240
           ILSRP IPVPFPDPAGD+SLLIGDWYSSNHTTLKACLDLGKKLPFPDGILINGRANYTSF
Sbjct: 181 ILSRPFIPVPFPDPAGDHSLLIGDWYSSNHTTLKACLDLGKKLPFPDGILINGRANYTSF 240

Query: 241 SVEQGKTYRLRISNVGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSVLV 300
           SVEQGKTYRLRISNVGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSVLV
Sbjct: 241 SVEQGKTYRLRISNVGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSVLV 300

Query: 301 TADQPAQDYYIVVSSRFTNPVLTTTGTLRYSNSTGPVKGPLPGAPTTEVGWSLNQARSIR 360
           TADQPAQDYYIVVSSRFTNPVLTTTG LRYSNSTGPVKGPLPGAPTTEVGWSLNQARSIR
Sbjct: 301 TADQPAQDYYIVVSSRFTNPVLTTTGILRYSNSTGPVKGPLPGAPTTEVGWSLNQARSIR 360

Query: 361 TNLTASGPRPNPQGSYHYGLVNTSRTIILESSAGLINSKQRYAVNRVSFIPADTPLKLAD 420
           TNLTASGPRPNPQGSYHYGLVNTSRTIILE+SAG+INSKQRYAVNRVSFIPADTPLKLAD
Sbjct: 361 TNLTASGPRPNPQGSYHYGLVNTSRTIILETSAGIINSKQRYAVNRVSFIPADTPLKLAD 420

Query: 421 FFKIGGVFRVGSISDRPTGGGMYLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFV 480
           FFKIGGV RVGSISDRPTGGGMYLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFV
Sbjct: 421 FFKIGGVLRVGSISDRPTGGGMYLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFV 480

Query: 481 VGMDGGQWSESSRGQYNLRDAIARCTIQVYPLSWTAIYMSLDNVGMWNMRSEFWARQYLG 540
           VGMDGGQWSESSRGQYNLRDAIARCTIQVYPLSWTAIYMSLDNVGMWN+RSEFWARQYLG
Sbjct: 481 VGMDGGQWSESSRGQYNLRDAIARCTIQVYPLSWTAIYMSLDNVGMWNIRSEFWARQYLG 540

Query: 541 QQFYLRVYTPSTSLRDEYPIPKNALLCGRASGRHTRPL 579
           QQFYLRVYTPSTS RDEYPIPKNALLCGRASGRHTRPL
Sbjct: 541 QQFYLRVYTPSTSFRDEYPIPKNALLCGRASGRHTRPL 541

BLAST of CmoCh01G020580 vs. NCBI nr
Match: KAG6608621.1 (hypothetical protein SDJN03_01963, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1083.2 bits (2800), Expect = 0.0e+00
Identity = 532/573 (92.84%), Postives = 536/573 (93.54%), Query Frame = 0

Query: 1   MLLLTSPAFALSAFLCFFAAVSADDPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDI 60
           MLLLTSPAFALSAFLCFFAAVSA+DPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDI
Sbjct: 1   MLLLTSPAFALSAFLCFFAAVSAEDPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDI 60

Query: 61  HCVTNDNLIINVFNSLDEPFLISWSLFLLSISLVFILLLTLFSCPPTPTPTPTPTPTLQR 120
           HCVTNDNLIINVFNSLDEPFLISW                                    
Sbjct: 61  HCVTNDNLIINVFNSLDEPFLISW------------------------------------ 120

Query: 121 RNGVQQRKNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIR 180
            NGVQQRKNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIR
Sbjct: 121 -NGVQQRKNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIR 180

Query: 181 ILSRPLIPVPFPDPAGDYSLLIGDWYSSNHTTLKACLDLGKKLPFPDGILINGRANYTSF 240
           ILSRPLIPVPFPDPAGDYSLLIGDWYSSNHTTLKACLDLGKKLPFPDGILINGRANYTSF
Sbjct: 181 ILSRPLIPVPFPDPAGDYSLLIGDWYSSNHTTLKACLDLGKKLPFPDGILINGRANYTSF 240

Query: 241 SVEQGKTYRLRISNVGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSVLV 300
           SVEQGKTYRLRISNVGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSVLV
Sbjct: 241 SVEQGKTYRLRISNVGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSVLV 300

Query: 301 TADQPAQDYYIVVSSRFTNPVLTTTGTLRYSNSTGPVKGPLPGAPTTEVGWSLNQARSIR 360
           TADQPAQDYYIVVSSRFTNPVLTTTGTLRYSNSTGPVKGPLPGAPTTEVGWSLNQARSIR
Sbjct: 301 TADQPAQDYYIVVSSRFTNPVLTTTGTLRYSNSTGPVKGPLPGAPTTEVGWSLNQARSIR 360

Query: 361 TNLTASGPRPNPQGSYHYGLVNTSRTIILESSAGLINSKQRYAVNRVSFIPADTPLKLAD 420
           TNLTASGPRPNPQGSYHYGLVNTSRTIILESSAGLINSKQRYAVNRVSFIPADTPLKLAD
Sbjct: 361 TNLTASGPRPNPQGSYHYGLVNTSRTIILESSAGLINSKQRYAVNRVSFIPADTPLKLAD 420

Query: 421 FFKIGGVFRVGSISDRPTGGGMYLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFV 480
           FFKIGGVFRVGSISDRPTGGGMYLDTSVMGADYRAFVEIVFQN+ENIVQSWHIDGYSFFV
Sbjct: 421 FFKIGGVFRVGSISDRPTGGGMYLDTSVMGADYRAFVEIVFQNSENIVQSWHIDGYSFFV 480

Query: 481 VGMDGGQWSESSRGQYNLRDAIARCTIQVYPLSWTAIYMSLDNVGMWNMRSEFWARQYLG 540
           VGMDGGQWSESSRGQYNLRDAIARCTIQVYPLSWTAIYMSLDNVGMWN+RSEFWARQYLG
Sbjct: 481 VGMDGGQWSESSRGQYNLRDAIARCTIQVYPLSWTAIYMSLDNVGMWNIRSEFWARQYLG 536

Query: 541 QQFYLRVYTPSTSLRDEYPIPKNALLCGRASGR 574
           QQFYLRVYTPSTSLRDEYPIPKNALLCGRASG+
Sbjct: 541 QQFYLRVYTPSTSLRDEYPIPKNALLCGRASGK 536

BLAST of CmoCh01G020580 vs. NCBI nr
Match: XP_022982319.1 (L-ascorbate oxidase homolog [Cucurbita maxima])

HSP 1 Score: 1070.1 bits (2766), Expect = 6.7e-309
Identity = 528/578 (91.35%), Postives = 534/578 (92.39%), Query Frame = 0

Query: 1   MLLLTSPAFALSAFLCFFAAVSADDPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDI 60
           MLLLTSPA ALSAFLCFFAAVSA++PYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDI
Sbjct: 1   MLLLTSPALALSAFLCFFAAVSAENPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDI 60

Query: 61  HCVTNDNLIINVFNSLDEPFLISWSLFLLSISLVFILLLTLFSCPPTPTPTPTPTPTLQR 120
           +CVTNDNLIINVFNSLDEPFLISW                                    
Sbjct: 61  YCVTNDNLIINVFNSLDEPFLISW------------------------------------ 120

Query: 121 RNGVQQRKNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIR 180
            NGVQQRKNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIR
Sbjct: 121 -NGVQQRKNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIR 180

Query: 181 ILSRPLIPVPFPDPAGDYSLLIGDWYSSNHTTLKACLDLGKKLPFPDGILINGRANYTSF 240
           ILSRPLIPVPFPDPAGDY+LL+GDWYSSNHTTLKA LDLGKKLPFPDGILING AN TSF
Sbjct: 181 ILSRPLIPVPFPDPAGDYTLLVGDWYSSNHTTLKARLDLGKKLPFPDGILINGGANRTSF 240

Query: 241 SVEQGKTYRLRISNVGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSVLV 300
            VEQGKTYRLRISNVGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSVLV
Sbjct: 241 IVEQGKTYRLRISNVGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSVLV 300

Query: 301 TADQPAQDYYIVVSSRFTNPVLTTTGTLRYSNSTGPVKGPLPGAPTTEVGWSLNQARSIR 360
           TADQPAQDYYIVVSSRFTNPVLTTTGTLRYSNSTGPVKGPLPGAPT EVGWSLNQARSIR
Sbjct: 301 TADQPAQDYYIVVSSRFTNPVLTTTGTLRYSNSTGPVKGPLPGAPTIEVGWSLNQARSIR 360

Query: 361 TNLTASGPRPNPQGSYHYGLVNTSRTIILESSAGLINSKQRYAVNRVSFIPADTPLKLAD 420
           TNLTASGPRPNPQGSYHYGLVNTSRTIILESSAGLINSKQRYAVNRVSFIPADTPLKLAD
Sbjct: 361 TNLTASGPRPNPQGSYHYGLVNTSRTIILESSAGLINSKQRYAVNRVSFIPADTPLKLAD 420

Query: 421 FFKIGGVFRVGSISDRPTGGGMYLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFV 480
           FFKIGGVFRVGSISDRPTGGGMYLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFV
Sbjct: 421 FFKIGGVFRVGSISDRPTGGGMYLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFV 480

Query: 481 VGMDGGQWSESSRGQYNLRDAIARCTIQVYPLSWTAIYMSLDNVGMWNMRSEFWARQYLG 540
           VGMDGGQWSESSRGQYNLRDAIAR TIQVYPLSWTAIYMSLDNVGMWN+RSEFWARQYLG
Sbjct: 481 VGMDGGQWSESSRGQYNLRDAIARSTIQVYPLSWTAIYMSLDNVGMWNIRSEFWARQYLG 540

Query: 541 QQFYLRVYTPSTSLRDEYPIPKNALLCGRASGRHTRPL 579
           QQFYLRVYTPSTSLRDEYPIPKNALLCGRASGRHTRPL
Sbjct: 541 QQFYLRVYTPSTSLRDEYPIPKNALLCGRASGRHTRPL 541

BLAST of CmoCh01G020580 vs. TAIR 10
Match: AT1G76160.1 (SKU5 similar 5 )

HSP 1 Score: 884.8 bits (2285), Expect = 3.8e-257
Identity = 423/575 (73.57%), Postives = 479/575 (83.30%), Query Frame = 0

Query: 6   SPAFALSAF--LCFFAAVSADDPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDIHCV 65
           S +FA + F  L    AV+A+DPYRFF WN++YGDIYPLG+RQQGILING FPGPDIH V
Sbjct: 4   SASFAAALFIGLSLLFAVTAEDPYRFFEWNITYGDIYPLGVRQQGILINGAFPGPDIHSV 63

Query: 66  TNDNLIINVFNSLDEPFLISWSLFLLSISLVFILLLTLFSCPPTPTPTPTPTPTLQRRNG 125
           TNDNLIINV+NSLDEPFL+SW                                     NG
Sbjct: 64  TNDNLIINVYNSLDEPFLLSW-------------------------------------NG 123

Query: 126 VQQRKNSYEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIRILS 185
           +QQR+NS+ DGVYGTTCPIPPGKN+TYILQ+KDQIGSFYYFPSL FHKAAGGFG IRILS
Sbjct: 124 IQQRRNSFVDGVYGTTCPIPPGKNYTYILQMKDQIGSFYYFPSLGFHKAAGGFGGIRILS 183

Query: 186 RPLIPVPFPDPAGDYSLLIGDWYSSNHTTLKACLDLGKKLPFPDGILINGRANYTSFSVE 245
           RP IPVPFPDPAGD ++LIGDWY +NHT L+A LD GKKLP PDGILINGR++  + +VE
Sbjct: 184 RPRIPVPFPDPAGDTTVLIGDWYKANHTDLRAQLDNGKKLPLPDGILINGRSSGATLNVE 243

Query: 246 QGKTYRLRISNVGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSVLVTAD 305
           QGKTYR RISNVGLQ SLNFRIQ H+MK+VEVEGTHTLQTT+SSLD+HVGQSYSVLVTAD
Sbjct: 244 QGKTYRFRISNVGLQDSLNFRIQDHKMKVVEVEGTHTLQTTFSSLDVHVGQSYSVLVTAD 303

Query: 306 QPAQDYYIVVSSRFTNPVLTTTGTLRYSNSTGPVKGPLPGAPTTEVGWSLNQARSIRTNL 365
           Q  +DYY+VVSSRFT+ VLTTTG  RYSNS G V GP+PG PT ++ WSLNQAR+IRTNL
Sbjct: 304 QTPRDYYVVVSSRFTSNVLTTTGIFRYSNSAGGVSGPIPGGPTIQIDWSLNQARAIRTNL 363

Query: 366 TASGPRPNPQGSYHYGLVNTSRTIILESSAGLINSKQRYAVNRVSFIPADTPLKLADFFK 425
           +ASGPRPNPQGSYHYG++NT+RTI L SSAG ++ KQRYAVN VSF PADTPLK+AD+FK
Sbjct: 364 SASGPRPNPQGSYHYGMINTTRTIRLASSAGQVDGKQRYAVNSVSFKPADTPLKIADYFK 423

Query: 426 IGGVFRVGSISDRPTGGGMYLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFVVGM 485
           I GV+R GSI  +PTGGG+YLDTSVM  DYR FVEI+F+N+E+IVQSWH+DGYSF+VVGM
Sbjct: 424 IDGVYRSGSIQYQPTGGGIYLDTSVMQVDYRTFVEIIFENSEDIVQSWHLDGYSFWVVGM 483

Query: 486 DGGQWSESSRGQYNLRDAIARCTIQVYPLSWTAIYMSLDNVGMWNMRSEFWARQYLGQQF 545
           DGGQWS  SR +YNLRDA+ARCT+QVYP SWTAI ++LDNVGMWN+RSEFWARQYLGQQ 
Sbjct: 484 DGGQWSPDSRNEYNLRDAVARCTVQVYPSSWTAILIALDNVGMWNLRSEFWARQYLGQQL 541

Query: 546 YLRVYTPSTSLRDEYPIPKNALLCGRASGRHTRPL 579
           YLRVYTPSTSLRDEYPIPKNALLCGRASGR TRPL
Sbjct: 544 YLRVYTPSTSLRDEYPIPKNALLCGRASGRSTRPL 541

BLAST of CmoCh01G020580 vs. TAIR 10
Match: AT1G41830.1 (SKU5-similar 6 )

HSP 1 Score: 868.2 bits (2242), Expect = 3.6e-252
Identity = 414/564 (73.40%), Postives = 465/564 (82.45%), Query Frame = 0

Query: 15  LCFFAAVSADDPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDIHCVTNDNLIINVFN 74
           L FFAAV+A+ PYRFF WNV+YGDIYPLG+RQQGILINGQFPGPDIH VTNDNLIINV N
Sbjct: 16  LSFFAAVTAESPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGPDIHSVTNDNLIINVHN 75

Query: 75  SLDEPFLISWSLFLLSISLVFILLLTLFSCPPTPTPTPTPTPTLQRRNGVQQRKNSYEDG 134
           SLDEPFLISW                                     NGVQ R+NSY DG
Sbjct: 76  SLDEPFLISW-------------------------------------NGVQNRRNSYVDG 135

Query: 135 VYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIRILSRPLIPVPFPDP 194
           +YGTTCPIPP  N+TYILQVKDQIGSFYYFPSLAFHKAAGGFG IRILSRP IPVPF DP
Sbjct: 136 MYGTTCPIPPRSNYTYILQVKDQIGSFYYFPSLAFHKAAGGFGGIRILSRPGIPVPFADP 195

Query: 195 AGDYSLLIGDWYSSNHTTLKACLDLGKKLPFPDGILINGRANYTSFSVEQGKTYRLRISN 254
           AGDY++LIGDWY  NHT LK+ LD G+KLP PDGILINGR+N  + +VEQGKTYRLRISN
Sbjct: 196 AGDYTVLIGDWYKFNHTDLKSRLDRGRKLPSPDGILINGRSNGATLNVEQGKTYRLRISN 255

Query: 255 VGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSVLVTADQPAQDYYIVVS 314
           VGLQ SLNFRIQ H+MKLVEVEGTHTLQT +SSLD+HVGQSYSVL+TADQ  +DYY+VVS
Sbjct: 256 VGLQDSLNFRIQNHRMKLVEVEGTHTLQTMFSSLDVHVGQSYSVLITADQSPRDYYVVVS 315

Query: 315 SRFTNPVLTTTGTLRYSNSTGPVKGPLPGAPTTEVGWSLNQARSIRTNLTASGPRPNPQG 374
           SRFT+ ++TTTG LRYS S+ P  GP+PG PT +V WSLNQAR+IRTNLTASGPRPNPQG
Sbjct: 316 SRFTDKIITTTGVLRYSGSSTPASGPIPGGPTIQVDWSLNQARAIRTNLTASGPRPNPQG 375

Query: 375 SYHYGLVNTSRTIILESSAGLINSKQRYAVNRVSFIPADTPLKLADFFKIGGVFRVGSIS 434
           SYHYGL+   RTI+  SSAG IN KQRY VN VSF+PADTPLKLADFFKI GV+++ SIS
Sbjct: 376 SYHYGLIPLIRTIVFGSSAGQINGKQRYGVNSVSFVPADTPLKLADFFKISGVYKINSIS 435

Query: 435 DRPTGGGMYLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFVVGMDGGQWSESSRG 494
           D+PT GG+YLDTSV+  DYR F+EIVF+N E+IVQS+H++GYSF+VVGMDGGQW   SR 
Sbjct: 436 DKPTYGGLYLDTSVLQVDYRTFIEIVFENQEDIVQSYHLNGYSFWVVGMDGGQWKTGSRN 495

Query: 495 QYNLRDAIARCTIQVYPLSWTAIYMSLDNVGMWNMRSEFWARQYLGQQFYLRVYTPSTSL 554
            YNLRDA++R T+QVYP SWTAIY++LDNVGMWN+RSEFWARQYLGQQ YLRV+T STSL
Sbjct: 496 GYNLRDAVSRSTVQVYPKSWTAIYIALDNVGMWNLRSEFWARQYLGQQLYLRVFTSSTSL 542

Query: 555 RDEYPIPKNALLCGRASGRHTRPL 579
           RDEYPIPKN+ LCGRA GRHTRPL
Sbjct: 556 RDEYPIPKNSRLCGRARGRHTRPL 542

BLAST of CmoCh01G020580 vs. TAIR 10
Match: AT1G21850.1 (SKU5 similar 8 )

HSP 1 Score: 810.1 bits (2091), Expect = 1.2e-234
Identity = 388/555 (69.91%), Postives = 446/555 (80.36%), Query Frame = 0

Query: 21  VSADDPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDIHCVTNDNLIINVFNSLDEPF 80
           V+A+DPY+FF W+V+YG+I PL + QQGILING+FPGPDI  VTNDNLIINVFN LDEPF
Sbjct: 22  VAAEDPYKFFEWHVTYGNISPLKVAQQGILINGKFPGPDIAAVTNDNLIINVFNHLDEPF 81

Query: 81  LISWSLFLLSISLVFILLLTLFSCPPTPTPTPTPTPTLQRRNGVQQRKNSYEDGVYGTTC 140
           LISWS                                     G++  +NSY+DGVYGTTC
Sbjct: 82  LISWS-------------------------------------GIRNWRNSYQDGVYGTTC 141

Query: 141 PIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIRILSRPLIPVPFPDPAGDYSL 200
           PIPPGKN+TY LQVKDQIGSFYYFPSL FHKAAGGFGAIRI SRP IPVPFP PAGDY++
Sbjct: 142 PIPPGKNYTYALQVKDQIGSFYYFPSLGFHKAAGGFGAIRISSRPRIPVPFPAPAGDYTV 201

Query: 201 LIGDWYSSNHTTLKACLDLGKKLPFPDGILINGRANYTSFSVEQGKTYRLRISNVGLQHS 260
           LIGDWY +NH  L+A LD G KLPFPDGILINGR +  + ++E GKTYRLRISNVGLQ+S
Sbjct: 202 LIGDWYKTNHKDLRAQLDNGGKLPFPDGILINGRGSGATLNIEPGKTYRLRISNVGLQNS 261

Query: 261 LNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSVLVTADQPAQDYYIVVSSRFTNP 320
           LNFRIQ H+MKLVEVEGTHT+QT +SSLD+HVGQSYSVL+TADQPA+DYYIVVSSRFT+ 
Sbjct: 262 LNFRIQNHKMKLVEVEGTHTIQTPFSSLDVHVGQSYSVLITADQPAKDYYIVVSSRFTSK 321

Query: 321 VLTTTGTLRYSNSTGPVKGPLPGAPTTEVGWSLNQARSIRTNLTASGPRPNPQGSYHYGL 380
           +L T G L YSNS GPV GP+P AP  ++ WS +QAR+I+TNL ASGPRPNPQG+YHYG 
Sbjct: 322 ILITAGVLHYSNSAGPVSGPIPEAP-IQLRWSFDQARAIKTNLAASGPRPNPQGTYHYGK 381

Query: 381 VNTSRTIILESSAGLINSKQRYAVNRVSFIPADTPLKLADFFKIGGVFRVGSISDRPTGG 440
           +  +RTI L SSAG IN KQRYAVN  SF P DTPLKLAD+FKI GV+  GSI D+PT G
Sbjct: 382 IKVTRTIKLASSAGNINGKQRYAVNSASFYPTDTPLKLADYFKIAGVYNPGSIPDQPTHG 441

Query: 441 GMYLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFVVGMDGGQWSESSRGQYNLRD 500
            +Y  TSVM  DY+AFVEIVF+N E+IVQ+WH+DGYSFFVVGM+ G+WS +SR  YNL D
Sbjct: 442 AIYPVTSVMQTDYKAFVEIVFENWEDIVQTWHLDGYSFFVVGMELGKWSAASRKVYNLND 501

Query: 501 AIARCTIQVYPLSWTAIYMSLDNVGMWNMRSEFWARQYLGQQFYLRVYTPSTSLRDEYPI 560
           A++RCT+QVYP SWTAIY+SLDNVGMWN+RSE W RQYLGQQFY+RVYTPSTSLRDEY I
Sbjct: 502 AVSRCTVQVYPRSWTAIYVSLDNVGMWNLRSELWERQYLGQQFYMRVYTPSTSLRDEYLI 538

Query: 561 PKNALLCGRASGRHT 576
           PKNALLCGRA+G HT
Sbjct: 562 PKNALLCGRATGHHT 538

BLAST of CmoCh01G020580 vs. TAIR 10
Match: AT1G21860.1 (SKU5 similar 7 )

HSP 1 Score: 793.9 bits (2049), Expect = 8.7e-230
Identity = 380/564 (67.38%), Postives = 443/564 (78.55%), Query Frame = 0

Query: 11  LSAFLCFFAAVSADDPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDIHCVTNDNLII 70
           ++  L   +   A+DPYRFF W+V+YG+I PLG+ QQGILING+FPGPDI  +TNDNLII
Sbjct: 12  ITTLLFLISLAFAEDPYRFFEWHVTYGNISPLGVAQQGILINGKFPGPDIISITNDNLII 71

Query: 71  NVFNSLDEPFLISWSLFLLSISLVFILLLTLFSCPPTPTPTPTPTPTLQRRNGVQQRKNS 130
           NVFN LDEPFL+SW                                     NG++  KNS
Sbjct: 72  NVFNHLDEPFLLSW-------------------------------------NGIRNWKNS 131

Query: 131 YEDGVYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIRILSRPLIPVP 190
           ++DGVYGT CPIPPGKN+TY LQVKDQIGSFYYFPSL FHKAAGGFG IRI SR LIPVP
Sbjct: 132 FQDGVYGTMCPIPPGKNYTYALQVKDQIGSFYYFPSLGFHKAAGGFGGIRISSRALIPVP 191

Query: 191 FPDPAGDYSLLIGDWYSSNHTTLKACLDLGKKLPFPDGILINGRANYTSFSVEQGKTYRL 250
           FP PA DY+LL+GDWY +NH  LKA LD G KLP PDGILINGR++  + ++E GKTYRL
Sbjct: 192 FPTPADDYTLLVGDWYKTNHKDLKAQLDNGGKLPLPDGILINGRSSGATLNIEPGKTYRL 251

Query: 251 RISNVGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSVLVTADQPAQDYY 310
           RISNVGLQ+SLNFRIQ H MKLVEVEG +T+Q  +SSLD+HVGQSYSVL+TADQPA+DYY
Sbjct: 252 RISNVGLQNSLNFRIQNHTMKLVEVEGRYTIQNLFSSLDVHVGQSYSVLITADQPAKDYY 311

Query: 311 IVVSSRFTNPVLTTTGTLRYSNSTGPVKGPLPGAPTTEVGWSLNQARSIRTNLTASGPRP 370
           +VVSSRFT+ +LTTTG L YSNS  PV GP+P  P  ++ WS NQAR+IRTNLTASGPRP
Sbjct: 312 VVVSSRFTSKILTTTGVLHYSNSVAPVSGPIPDGP-IKLSWSFNQARAIRTNLTASGPRP 371

Query: 371 NPQGSYHYGLVNTSRTIILESSAGLINSKQRYAVNRVSFIPADTPLKLADFFKIGGVFRV 430
           NPQGSY YG++N +RTI L ++ G I  KQRYAVN  SF PADTPLKL D+FKI GV++ 
Sbjct: 372 NPQGSYRYGVINITRTIRLANNLGHIEGKQRYAVNSASFYPADTPLKLVDYFKIDGVYKP 431

Query: 431 GSISDRPTGGGMYLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFVVGMDGGQWSE 490
           GSISD+PT G ++  TSVM AD+RAFVE++F+N+E+IVQSWH+DGYSF+VVGM+ G+WS 
Sbjct: 432 GSISDQPTNGAIFPTTSVMQADFRAFVEVIFENSEDIVQSWHLDGYSFYVVGMELGKWSP 491

Query: 491 SSRGQYNLRDAIARCTIQVYPLSWTAIYMSLDNVGMWNMRSEFWARQYLGQQFYLRVYTP 550
           +SR  YNL DAI RCTIQVYP SWTAIY++LDNVGMWNMRSE W RQYLGQQFY+RVYT 
Sbjct: 492 ASRKVYNLNDAILRCTIQVYPRSWTAIYIALDNVGMWNMRSEIWERQYLGQQFYMRVYTT 537

Query: 551 STSLRDEYPIPKNALLCGRASGRH 575
           STSLRDEY IPKNALLCGRAS  H
Sbjct: 552 STSLRDEYLIPKNALLCGRASSSH 537

BLAST of CmoCh01G020580 vs. TAIR 10
Match: AT4G22010.1 (SKU5 similar 4 )

HSP 1 Score: 780.4 bits (2014), Expect = 1.0e-225
Identity = 379/565 (67.08%), Postives = 434/565 (76.81%), Query Frame = 0

Query: 15  LCFFAAVSADDPYRFFTWNVSYGDIYPLGLRQQGILINGQFPGPDIHCVTNDNLIINVFN 74
           L     V  D+PYRFFTW ++YGDIYPLG++QQGILINGQFPGP I  +TNDN+II+VFN
Sbjct: 13  LVLINGVLGDNPYRFFTWKITYGDIYPLGVKQQGILINGQFPGPHIDAITNDNIIISVFN 72

Query: 75  SLDEPFLISWSLFLLSISLVFILLLTLFSCPPTPTPTPTPTPTLQRRNGVQQRKNSYEDG 134
            L EPFLISW                                     NGVQQRKNS++DG
Sbjct: 73  YLKEPFLISW-------------------------------------NGVQQRKNSWQDG 132

Query: 135 VYGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLAFHKAAGGFGAIRILSRPLIPVPFPDP 194
           V GTTCPIPPGKNFTY++QVKDQIGSFYYFPSLAFHKAAG FGAIR+ SRP IPVPF  P
Sbjct: 133 VVGTTCPIPPGKNFTYVIQVKDQIGSFYYFPSLAFHKAAGAFGAIRVWSRPRIPVPFSPP 192

Query: 195 AGDYSLLIGDWYSSNHTTLKACLDLGKKLPFPDGILINGRA-NYTSFSVEQGKTYRLRIS 254
            GD+ LL GDWY +NH  L+  L+ G+ LP PDG+LINGR     +F+V+ GKTYR RIS
Sbjct: 193 DGDFWLLAGDWYKTNHYVLRRLLEAGRNLPNPDGVLINGRGWGGNTFTVQPGKTYRFRIS 252

Query: 255 NVGLQHSLNFRIQGHQMKLVEVEGTHTLQTTYSSLDIHVGQSYSVLVTADQPAQDYYIVV 314
           NVG+  SLNFRIQGH MKLVEVEG+HT+Q  Y+SLDIH+GQSYSVLVTA+Q  QDYYIV+
Sbjct: 253 NVGVATSLNFRIQGHTMKLVEVEGSHTVQNIYTSLDIHLGQSYSVLVTANQAPQDYYIVI 312

Query: 315 SSRFTNPVLTTTGTLRYSNSTGPVKGPLPGAPTTEVGWSLNQARSIRTNLTASGPRPNPQ 374
           SSRFT  VLTTT  L YSNS   V GP+P  PT ++  SL QAR+IR NLTASGPRPNPQ
Sbjct: 313 SSRFTRKVLTTTSILHYSNSRKGVSGPVPNGPTLDIASSLYQARTIRRNLTASGPRPNPQ 372

Query: 375 GSYHYGLVNTSRTIILESSAGLINSKQRYAVNRVSFIPADTPLKLADFFKIGGVFRVGSI 434
           GSYHYGL+   RTIIL +SA  IN KQRYAVN  SF+  DTPLKLAD+FKI GVF +GSI
Sbjct: 373 GSYHYGLIKPGRTIILANSAPWINGKQRYAVNGASFVAPDTPLKLADYFKIPGVFNLGSI 432

Query: 435 SDRPTGG-GMYLDTSVMGADYRAFVEIVFQNNENIVQSWHIDGYSFFVVGMDGGQWSESS 494
              P+GG G YL +SVM A++R F+E+VFQN EN VQSWH+ GYSFFVVGMDGGQW+  S
Sbjct: 433 PTSPSGGNGGYLQSSVMAANFREFIEVVFQNWENSVQSWHVSGYSFFVVGMDGGQWTPGS 492

Query: 495 RGQYNLRDAIARCTIQVYPLSWTAIYMSLDNVGMWNMRSEFWARQYLGQQFYLRVYTPST 554
           R +YNLRDA++R T+QVYP +WTAIY++LDNVGMWN+RSE WARQYLGQQFYLRVYT ST
Sbjct: 493 RAKYNLRDAVSRSTVQVYPRAWTAIYIALDNVGMWNIRSENWARQYLGQQFYLRVYTSST 540

Query: 555 SLRDEYPIPKNALLCGRASGRHTRP 578
           S RDEYP PKNAL+CGRA GRHTRP
Sbjct: 553 SYRDEYPPPKNALMCGRAKGRHTRP 540

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P291622.3e-16649.83L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q006248.1e-16450.72L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1[more]
Q8VXX56.0e-15147.86Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=... [more]
Q9SU407.4e-14948.19Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=... [more]
Q9FHN61.9e-14446.05Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1FJ990.0e+0093.60L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111445880 PE=3 S... [more]
A0A6J1J4693.2e-30991.35L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111481188 PE=3 SV=... [more]
A0A5A7VBL13.8e-28983.74L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
E5GCD63.8e-28983.74Multicopper oxidase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
A0A1S3BUY93.8e-28983.74L-ascorbate oxidase homolog OS=Cucumis melo OX=3656 GN=LOC103493793 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022940174.10.0e+0093.60L-ascorbate oxidase homolog [Cucurbita moschata][more]
KAG7037936.10.0e+0093.08hypothetical protein SDJN02_01569, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_023525181.10.0e+0091.87L-ascorbate oxidase homolog [Cucurbita pepo subsp. pepo][more]
KAG6608621.10.0e+0092.84hypothetical protein SDJN03_01963, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022982319.16.7e-30991.35L-ascorbate oxidase homolog [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT1G76160.13.8e-25773.57SKU5 similar 5 [more]
AT1G41830.13.6e-25273.40SKU5-similar 6 [more]
AT1G21850.11.2e-23469.91SKU5 similar 8 [more]
AT1G21860.18.7e-23067.38SKU5 similar 7 [more]
AT4G22010.11.0e-22567.08SKU5 similar 4 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 422..550
e-value: 5.5E-27
score: 94.2
IPR008972CupredoxinGENE3D2.60.40.420coord: 379..574
e-value: 1.1E-33
score: 118.7
IPR008972CupredoxinGENE3D2.60.40.420coord: 195..368
e-value: 4.2E-38
score: 133.4
IPR008972CupredoxinGENE3D2.60.40.420coord: 27..185
e-value: 2.3E-26
score: 94.3
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 22..211
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 191..370
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 380..569
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 197..332
e-value: 2.1E-41
score: 141.7
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 34..85
e-value: 3.5E-11
score: 43.2
coord: 121..184
e-value: 2.0E-17
score: 63.3
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 119..571
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 15..85
NoneNo IPR availablePANTHERPTHR11709:SF267MULTI-COPPER OXIDASE TYPE I FAMILY PROTEIN-RELATEDcoord: 15..85
NoneNo IPR availablePANTHERPTHR11709:SF267MULTI-COPPER OXIDASE TYPE I FAMILY PROTEIN-RELATEDcoord: 119..571
IPR034275Ascorbate oxidase homologue, third cupredoxin domainCDDcd13894CuRO_3_AAO_like_1coord: 411..532
e-value: 1.02052E-71
score: 223.846

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh01G020580.1CmoCh01G020580.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005507 copper ion binding
molecular_function GO:0016491 oxidoreductase activity