CmoCh01G018570 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh01G018570
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	3-oxo-Delta(4,5)-steroid 5-beta-reductase
Location	Cmo_Chr01: 13503775 .. 13505613 (-)
RNA-Seq Expression	CmoCh01G018570
Synteny	CmoCh01G018570

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAAAAAAAAAAAAAAAAGGAAGTAATGACCTTCCATAAACATTCGTTTCTGATATTGGTAGATGGTTACATAGGAAAATCCAGTCGGGAGTCCAGCGGTTCGGGTTGAATGTTTATCCTTTCACAGCTCGCGTCATGGTTGGCGTTTCCTCTGTAATCCACAAGACAACAACGATCTGTAACATCATCACCTTATAGCCCTTTCAACACACGATTCAATTCCACTTCAATCTCTGTTTCGCATCTTCCCCTCCTCCCTAAATCCCCAAAACCTATCTCCGATTCCCCGACGGAAAATGAGCTGGTGGTGGGCCGGAGCAATCGGCGCTGCTAAGGTAATTAAAACCCCTAACTCCCTATCTATGCCCCTGTTTCTATTACGTTTCTCTAATCAGCATCTCCGTTCCCAATTTTGCAGAAGAAATTTGAAGAAGATGAACAACCGAGGAGCTTTGAGAGCGTAGCATTGGTGATCGGCGTTACAGGCATCGTCGGCAACAGCCTTGCCGAGATTCTCCCTCTTTCCGACACCCCCGGCGGTCCGTGGAAGGTGTACGGCGTCGCTCGGAGGCCGAGGCCAAACTGGAACGCCGACCATCCCATCGAATACATCCAATGCGATGTCTCCGATCCGGAAGACGCCGAAGCCAAACTCTCGAAGCTCACTGATGTGACTCATGTGTTCTATGTCACATGGACCAATCTACCCACAGAGATCGAGAACTGTGAGGTCAACGGAAAAATGCTTCGCAACGTGTTGCGATCGGTGATTCCGAACGCGCCAAATCTCCGCCATATCTGCCTTCAAACAGGTACGAAGCACTACATCGGATCGTTCGAGAACATCATCGATCATCCGACGCAGCGGCACGATCCGCCTTTCACGGAGGACATGCCTCGATTGGACTCCCCAAATTTCTATTACACTCAAGAAGATTTGTTGTTCGAAGAGATCGAAAAGAGTCAGAAAAAGGATCTGACATGGGCGGTGATCCGACCCAACACGATCTTCGGGTTTTCCCCTTTCAGCTTGATGAACATCGTCGGAACCCTATGCACGTACGCCGCCATATGCAAGCACGAGGGACGTCCGTTGAAATTCCCCGGCACGAAATCAGCTTGGGAGGGTTACTCAGTGGTCTCCGACGCAGATCTGATAGCGGAGCAGCACATATGGACGGCGGTAGATCCATACGCAAAGAACGAGGCGTTTAACTGCAGCAATGGCGATGTATTCAAGTGGAAACACTTCTGGAAGGTTTTGGCGGAGCAGTTCAACATCGAGGATTATGGGTTCGAAGAGGGTGAGAGATTGCCGCTGGGAGAGTTGATGAAGGGGAAAAATGCAGTCTGGGAAGAAATTGTGAAGGAGAATCAGTTGCAACCAACAAAATTGGAGGATGTTGCTAATTGGTGGCATACAGATATAGCTTTGAGCGCTTCTGGTAACATAGATAGTATGAACAAGAGCAAGGAGCATGGATTCTTGGGCTTCAGAAACTCCAAGAACTCCTTCATTTCTTGGATCGACAAGGTTAAGGCTTTCAAAATCGTGCCCTGATTGCCGAGTAATGTCCATAATTATCAATCTTCTCGTACTGATTTTCATTTCTGGAATCTGTTTGGTTTGGGTTGCTATGGTTTTACAATAAATGTTGGCTTGTTTATATAGTTTCTTGGTATCTTAGTGACGAGTTCTTGGTAATTCATAGTGTTCCAGCTAGGGAATGATTTCAGTTTGTTTTATGCTTTTCACTCAATAAGTAATAATCTTTAGAACGTTGTTACATTACTGGCTGTCTAATTGTTCGATTCTTGTCACAAACCACAGTTTCCT

mRNA sequence

AAAAAAAAAAAAAAAAAAAAGGAAGTAATGACCTTCCATAAACATTCGTTTCTGATATTGGTAGATGGTTACATAGGAAAATCCAGTCGGGAGTCCAGCGGTTCGGGTTGAATGTTTATCCTTTCACAGCTCGCGTCATGGTTGGCGTTTCCTCTGTAATCCACAAGACAACAACGATCTGTAACATCATCACCTTATAGCCCTTTCAACACACGATTCAATTCCACTTCAATCTCTGTTTCGCATCTTCCCCTCCTCCCTAAATCCCCAAAACCTATCTCCGATTCCCCGACGGAAAATGAGCTGGTGGTGGGCCGGAGCAATCGGCGCTGCTAAGAAGAAATTTGAAGAAGATGAACAACCGAGGAGCTTTGAGAGCGTAGCATTGGTGATCGGCGTTACAGGCATCGTCGGCAACAGCCTTGCCGAGATTCTCCCTCTTTCCGACACCCCCGGCGGTCCGTGGAAGGTGTACGGCGTCGCTCGGAGGCCGAGGCCAAACTGGAACGCCGACCATCCCATCGAATACATCCAATGCGATGTCTCCGATCCGGAAGACGCCGAAGCCAAACTCTCGAAGCTCACTGATGTGACTCATGTGTTCTATGTCACATGGACCAATCTACCCACAGAGATCGAGAACTGTGAGGTCAACGGAAAAATGCTTCGCAACGTGTTGCGATCGGTGATTCCGAACGCGCCAAATCTCCGCCATATCTGCCTTCAAACAGGTACGAAGCACTACATCGGATCGTTCGAGAACATCATCGATCATCCGACGCAGCGGCACGATCCGCCTTTCACGGAGGACATGCCTCGATTGGACTCCCCAAATTTCTATTACACTCAAGAAGATTTGTTGTTCGAAGAGATCGAAAAGAGTCAGAAAAAGGATCTGACATGGGCGGTGATCCGACCCAACACGATCTTCGGGTTTTCCCCTTTCAGCTTGATGAACATCGTCGGAACCCTATGCACGTACGCCGCCATATGCAAGCACGAGGGACGTCCGTTGAAATTCCCCGGCACGAAATCAGCTTGGGAGGGTTACTCAGTGGTCTCCGACGCAGATCTGATAGCGGAGCAGCACATATGGACGGCGGTAGATCCATACGCAAAGAACGAGGCGTTTAACTGCAGCAATGGCGATGTATTCAAGTGGAAACACTTCTGGAAGGTTTTGGCGGAGCAGTTCAACATCGAGGATTATGGGTTCGAAGAGGGTGAGAGATTGCCGCTGGGAGAGTTGATGAAGGGGAAAAATGCAGTCTGGGAAGAAATTGTGAAGGAGAATCAGTTGCAACCAACAAAATTGGAGGATGTTGCTAATTGGTGGCATACAGATATAGCTTTGAGCGCTTCTGGTAACATAGATAGTATGAACAAGAGCAAGGAGCATGGATTCTTGGGCTTCAGAAACTCCAAGAACTCCTTCATTTCTTGGATCGACAAGGTTAAGGCTTTCAAAATCGTGCCCTGATTGCCGAGTAATGTCCATAATTATCAATCTTCTCGTACTGATTTTCATTTCTGGAATCTGTTTGGTTTGGGTTGCTATGGTTTTACAATAAATGTTGGCTTGTTTATATAGTTTCTTGGTATCTTAGTGACGAGTTCTTGGTAATTCATAGTGTTCCAGCTAGGGAATGATTTCAGTTTGTTTTATGCTTTTCACTCAATAAGTAATAATCTTTAGAACGTTGTTACATTACTGGCTGTCTAATTGTTCGATTCTTGTCACAAACCACAGTTTCCT

Coding sequence (CDS)

ATGAGCTGGTGGTGGGCCGGAGCAATCGGCGCTGCTAAGAAGAAATTTGAAGAAGATGAACAACCGAGGAGCTTTGAGAGCGTAGCATTGGTGATCGGCGTTACAGGCATCGTCGGCAACAGCCTTGCCGAGATTCTCCCTCTTTCCGACACCCCCGGCGGTCCGTGGAAGGTGTACGGCGTCGCTCGGAGGCCGAGGCCAAACTGGAACGCCGACCATCCCATCGAATACATCCAATGCGATGTCTCCGATCCGGAAGACGCCGAAGCCAAACTCTCGAAGCTCACTGATGTGACTCATGTGTTCTATGTCACATGGACCAATCTACCCACAGAGATCGAGAACTGTGAGGTCAACGGAAAAATGCTTCGCAACGTGTTGCGATCGGTGATTCCGAACGCGCCAAATCTCCGCCATATCTGCCTTCAAACAGGTACGAAGCACTACATCGGATCGTTCGAGAACATCATCGATCATCCGACGCAGCGGCACGATCCGCCTTTCACGGAGGACATGCCTCGATTGGACTCCCCAAATTTCTATTACACTCAAGAAGATTTGTTGTTCGAAGAGATCGAAAAGAGTCAGAAAAAGGATCTGACATGGGCGGTGATCCGACCCAACACGATCTTCGGGTTTTCCCCTTTCAGCTTGATGAACATCGTCGGAACCCTATGCACGTACGCCGCCATATGCAAGCACGAGGGACGTCCGTTGAAATTCCCCGGCACGAAATCAGCTTGGGAGGGTTACTCAGTGGTCTCCGACGCAGATCTGATAGCGGAGCAGCACATATGGACGGCGGTAGATCCATACGCAAAGAACGAGGCGTTTAACTGCAGCAATGGCGATGTATTCAAGTGGAAACACTTCTGGAAGGTTTTGGCGGAGCAGTTCAACATCGAGGATTATGGGTTCGAAGAGGGTGAGAGATTGCCGCTGGGAGAGTTGATGAAGGGGAAAAATGCAGTCTGGGAAGAAATTGTGAAGGAGAATCAGTTGCAACCAACAAAATTGGAGGATGTTGCTAATTGGTGGCATACAGATATAGCTTTGAGCGCTTCTGGTAACATAGATAGTATGAACAAGAGCAAGGAGCATGGATTCTTGGGCTTCAGAAACTCCAAGAACTCCTTCATTTCTTGGATCGACAAGGTTAAGGCTTTCAAAATCGTGCCCTGA

Protein sequence

MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNGKMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNFYYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLKFPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFNIEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDSMNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP

Homology

[Close]

BLAST of CmoCh01G018570 vs. ExPASy Swiss-Prot
Match: Q9STX2 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP1 PE=1 SV=1)

HSP 1 Score: 652.9 bits (1683), Expect = 2.3e-186
Identity = 303/393 (77.10%), Postives = 334/393 (84.99%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKK +EDE  +SFESVAL+IGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKLDEDEPSQSFESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP WNADHPI+YIQCDVSD ED  +KLS LTDVTHVFYVTWTN  +E ENCE NG
Sbjct: 61  VARRPRPTWNADHPIDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNRESESENCEANG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
            MLRNVL+++IP APNLRH+CLQTGTKHY+G F N +D P  RHDPPFTEDMPRL   NF
Sbjct: 121 SMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPFTN-VDGP--RHDPPFTEDMPRLQIQNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQED+LFEEI+K +   +TW++ RPN IFGFSP+SLMNIVGTLC YAAICKHEG PL 
Sbjct: 181 YYTQEDILFEEIKKIE--TVTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLL 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPG+K AWEG+   SDADLIAEQ IW AVDPYAKNEAFNC+N D+FKWKH WK+LAEQF 
Sbjct: 241 FPGSKKAWEGFMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKILAEQFG 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IE+YGFEEG+ L L E+MKGK  VWEE+VKENQLQ  KLE+V  WW  D+ L   G IDS
Sbjct: 301 IEEYGFEEGKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEGMIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKE+GFLGFRNS NSFISWIDK KAFKIVP
Sbjct: 361 MNKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 388

[Close]

BLAST of CmoCh01G018570 vs. ExPASy Swiss-Prot
Match: A0A221J5W8 ((S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta cataria OX=39347 GN=ISY1 PE=1 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 2.1e-179
Identity = 291/393 (74.05%), Postives = 330/393 (83.97%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKK +EDE PR++ESVAL++GVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKIDEDEAPRNYESVALIVGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP+WN DHPI YI CDV D  D EAKLS LTDVTH+FY TWT   TE ENCE NG
Sbjct: 61  VARRPRPSWNEDHPITYISCDVLDSVDVEAKLSPLTDVTHIFYATWTKRSTEKENCEANG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
           KML+NVL ++IPN PNL+HICLQTG KHY+G+FE   D  ++ HDPP TED+PRLDS N+
Sbjct: 121 KMLKNVLNAMIPNCPNLKHICLQTGRKHYLGAFE---DLKSKCHDPPLTEDLPRLDSQNY 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQED+LFEE++K  K+ LTW+V RP TIFGFSP+S+MN+VGTLC YAAICKHEG  L+
Sbjct: 181 YYTQEDILFEEVQK--KEGLTWSVHRPGTIFGFSPYSMMNLVGTLCVYAAICKHEGAVLR 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPG K AW+GYS  SDADLIAE  IW AVDPYAKNEAFN SNGDVFKWKHFWKVLAEQF 
Sbjct: 241 FPGCKGAWDGYSDCSDADLIAEHQIWAAVDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFG 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           +E   +EEG+++ L +LMK K  VW++IV+EN L  TKLEDV NWW +DI L     +D+
Sbjct: 301 VECGEYEEGQQVKLQDLMKDKGPVWDKIVRENGLSTTKLEDVGNWWFSDIVLGNECWLDT 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 388

[Close]

BLAST of CmoCh01G018570 vs. ExPASy Swiss-Prot
Match: A0A221J5X3 ((S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta racemosa OX=54731 GN=ISY1 PE=1 SV=1)

HSP 1 Score: 628.6 bits (1620), Expect = 4.6e-179
Identity = 291/393 (74.05%), Postives = 328/393 (83.46%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKK+ +EDE PR++ESVAL++GVTGIVGNSLAEILPLSDTP GPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKRIDEDEAPRNYESVALIVGVTGIVGNSLAEILPLSDTPCGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP+WN DHPI YI CDV D  D EAKLS LTDVTH+FY TWT   TE ENCE NG
Sbjct: 61  VARRPRPSWNEDHPITYISCDVLDSVDVEAKLSPLTDVTHIFYATWTKRSTEKENCEANG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
           KML+NVL ++IPN PNL+HICLQTG KHY+G+FE   D   +RHDPP TED+PRLDS NF
Sbjct: 121 KMLKNVLNAMIPNCPNLKHICLQTGRKHYLGAFE---DWKIKRHDPPLTEDLPRLDSQNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQED+LFEE++K  K+ LTW+V RP TIFGFSP+S+MN+VGTLC YAAICKHEG  L+
Sbjct: 181 YYTQEDILFEEVQK--KESLTWSVHRPGTIFGFSPYSMMNLVGTLCVYAAICKHEGAVLR 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPG K AW+GYS  SDADLIAE  IW AVDPYAKNEAFN SNGDVFKWKHFWKVLAEQF 
Sbjct: 241 FPGCKGAWDGYSDCSDADLIAEHQIWAAVDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFG 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           +E   +EEG+ + L +LMK K  VW++IV+EN L  TKLEDV NWW +DI L     +D+
Sbjct: 301 VECGEYEEGKEVKLQDLMKDKGPVWDKIVRENGLSTTKLEDVGNWWFSDIVLGNECWLDT 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 388

[Close]

BLAST of CmoCh01G018570 vs. ExPASy Swiss-Prot
Match: A0A1C9CX66 ((S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC2 PE=1 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 1.5e-169
Identity = 283/394 (71.83%), Postives = 314/394 (79.70%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAA+KKFEED+ PR  +SVAL+IGVTGIVGNSLAEILP+SDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAARKKFEEDDAPRDCQSVALIIGVTGIVGNSLAEILPISDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP WNADHP+EYIQCD+SD  D   KLS LTDVTH+F+VTW N PTE E CE+NG
Sbjct: 61  VARRPRPAWNADHPVEYIQCDISDASDTHTKLSPLTDVTHIFWVTWANRPTESECCELNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
            MLRNVL ++IP A NL HICLQTG KHYIG FE       + H+PPFTEDMPRL++PNF
Sbjct: 121 TMLRNVLNALIPKAANLHHICLQTGHKHYIGPFEAF--GKIKPHEPPFTEDMPRLNAPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYT ED+L E  EK  K  L W+V RP  IFGFSPFS+MNI+GTLC YAAICKHE  PLK
Sbjct: 181 YYTLEDMLVEASEK--KAGLNWSVHRPAVIFGFSPFSMMNIIGTLCVYAAICKHENTPLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGTK+AW  YSV SDADLIAE  IW AVDPYAKNEAFNCSNGD+FKWKH WKVLAEQF 
Sbjct: 241 FPGTKAAWNCYSVASDADLIAEHQIWAAVDPYAKNEAFNCSNGDLFKWKHLWKVLAEQFG 300

Query: 301 IEDYGFEEGER-LPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNID 360
           +E   F+E E+   L E MK K  VWEEIV+EN L  TKLE VA WW  D+ L     +D
Sbjct: 301 VEYAEFDESEKPTSLVERMKDKGPVWEEIVRENGLHTTKLEGVATWWFADVILGGECLLD 360

Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           SMNKSKEHG+LGFRN+KNS IS IDK+KA KIVP
Sbjct: 361 SMNKSKEHGYLGFRNTKNSLISVIDKMKAHKIVP 390

[Close]

BLAST of CmoCh01G018570 vs. ExPASy Swiss-Prot
Match: Q6PQJ9 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata OX=49450 PE=1 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 7.4e-169
Identity = 276/393 (70.23%), Postives = 317/393 (80.66%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKK+ EED+      SVAL++GVTGI+GNSLAEILPL+DTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKRLEEDDAQPKHSSVALIVGVTGIIGNSLAEILPLADTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARR RP W+ D+PI Y+QCD+SDP+D++AKLS LTDVTHVFYVTW N  TE ENCE N 
Sbjct: 61  VARRTRPAWHEDNPINYVQCDISDPDDSQAKLSPLTDVTHVFYVTWANRSTEQENCEANS 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
           KM RNVL +VIPN PNL+HI LQTG KHY+G FE+      + HDPP+TED+PRL   NF
Sbjct: 121 KMFRNVLDAVIPNCPNLKHISLQTGRKHYMGPFESY--GKIESHDPPYTEDLPRLKYMNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YY  ED++ EE+EK  K+ LTW+V RP  IFGFSP+S+MN+VGTLC YAAICKHEG+ L+
Sbjct: 181 YYDLEDIMLEEVEK--KEGLTWSVHRPGNIFGFSPYSMMNLVGTLCVYAAICKHEGKVLR 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           F G K+AW+GYS  SDADLIAE HIW AVDPYAKNEAFN SNGDVFKWKHFWKVLAEQF 
Sbjct: 241 FTGCKAAWDGYSDCSDADLIAEHHIWAAVDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFG 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           +    +EEG  L L +LMKGK  VWEEIV+EN L PTKL+DV  WW  D+ L     +DS
Sbjct: 301 VGCGEYEEGVDLKLQDLMKGKEPVWEEIVRENGLTPTKLKDVGIWWFGDVILGNECFLDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKEHGFLGFRNSKN+FISWIDK KA+KIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNAFISWIDKAKAYKIVP 389

[Close]

BLAST of CmoCh01G018570 vs. ExPASy TrEMBL
Match: A0A6J1FQC1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucurbita moschata OX=3662 GN=LOC111445997 PE=3 SV=1)

HSP 1 Score: 837.0 bits (2161), Expect = 3.2e-239
Identity = 393/393 (100.00%), Postives = 393/393 (100.00%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG
Sbjct: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK
Sbjct: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS
Sbjct: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393

[Close]

BLAST of CmoCh01G018570 vs. ExPASy TrEMBL
Match: A0A6J1J0N7 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111480841 PE=3 SV=1)

HSP 1 Score: 827.4 bits (2136), Expect = 2.5e-236
Identity = 387/393 (98.47%), Postives = 391/393 (99.49%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIG+TGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGITGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENC+VNG
Sbjct: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCDVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIID+P+ RHDPPFTEDMPRLDSPNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPSHRHDPPFTEDMPRLDSPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK
Sbjct: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IEDYGFEEGERLPLGELMKGKN VWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS
Sbjct: 301 IEDYGFEEGERLPLGELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393

[Close]

BLAST of CmoCh01G018570 vs. ExPASy TrEMBL
Match: A0A0B5CYJ8 (Putative progesterone 5-beta-reductase OS=Cucurbita pepo OX=3663 PE=2 SV=1)

HSP 1 Score: 818.5 bits (2113), Expect = 1.2e-233
Identity = 386/393 (98.22%), Postives = 388/393 (98.73%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           M WWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MRWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRPNWNADH IEYIQCDVSD EDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG
Sbjct: 61  VARRPRPNWNADHLIEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIID+PTQRHDPPFTEDMPRLD+PNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK
Sbjct: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IEDYGFEEGERLPL ELMKGKN VWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS
Sbjct: 301 IEDYGFEEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393

[Close]

BLAST of CmoCh01G018570 vs. ExPASy TrEMBL
Match: A0A5A7T3B3 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G001350 PE=3 SV=1)

HSP 1 Score: 737.3 bits (1902), Expect = 3.4e-209
Identity = 337/393 (85.75%), Postives = 364/393 (92.62%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDE PRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP WNADHPIEYIQCDVSDP+DAE KLS+L DVTH+FYVTWTN   EI+NCE N 
Sbjct: 61  VARRPRPVWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTIEIDNCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
           +MLRNVLRSVIPNAPNLRHICLQTGTKHY+GSFE++I+  +QRH+PPFTED+PRL+ PNF
Sbjct: 121 EMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESVINKSSQRHEPPFTEDLPRLECPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YY QEDLLFEEIE+SQKKDLTWAVIRPN IFGFSPFSLMNIVGTLC YAAICKHEGRPLK
Sbjct: 181 YYKQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGTK AWE +   SDADL+AEQHIWTAVDPY+KNEAFNC+NGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKLAWEDFQAASDADLVAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IE+YGFEEGERL L +LMK K+ VW+EIVKENQLQPTKLE++ NWW+ D+  S SGNIDS
Sbjct: 301 IEEYGFEEGERLTLVDLMKDKSDVWDEIVKENQLQPTKLEEIGNWWYVDVIFSVSGNIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKEHGFLGFRNSKNSFISWIDK+KAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393

[Close]

BLAST of CmoCh01G018570 vs. ExPASy TrEMBL
Match: A0A1S3BHA7 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo OX=3656 GN=LOC103489839 PE=3 SV=1)

HSP 1 Score: 737.3 bits (1902), Expect = 3.4e-209
Identity = 337/393 (85.75%), Postives = 364/393 (92.62%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDE PRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP WNADHPIEYIQCDVSDP+DAE KLS+L DVTH+FYVTWTN   EI+NCE N 
Sbjct: 61  VARRPRPVWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTIEIDNCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
           +MLRNVLRSVIPNAPNLRHICLQTGTKHY+GSFE++I+  +QRH+PPFTED+PRL+ PNF
Sbjct: 121 EMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESVINKSSQRHEPPFTEDLPRLECPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YY QEDLLFEEIE+SQKKDLTWAVIRPN IFGFSPFSLMNIVGTLC YAAICKHEGRPLK
Sbjct: 181 YYKQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGTK AWE +   SDADL+AEQHIWTAVDPY+KNEAFNC+NGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKLAWEDFQAASDADLVAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IE+YGFEEGERL L +LMK K+ VW+EIVKENQLQPTKLE++ NWW+ D+  S SGNIDS
Sbjct: 301 IEEYGFEEGERLTLVDLMKDKSDVWDEIVKENQLQPTKLEEIGNWWYVDVIFSVSGNIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKEHGFLGFRNSKNSFISWIDK+KAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393

[Close]

BLAST of CmoCh01G018570 vs. NCBI nr
Match: XP_022940360.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucurbita moschata] >KAG6608438.1 3-oxo-Delta(4,5)-steroid 5-beta-reductase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037773.1 3-oxo-Delta(4,5)-steroid 5-beta-reductase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 837.0 bits (2161), Expect = 6.6e-239
Identity = 393/393 (100.00%), Postives = 393/393 (100.00%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG
Sbjct: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK
Sbjct: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS
Sbjct: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393

[Close]

BLAST of CmoCh01G018570 vs. NCBI nr
Match: XP_022981805.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita maxima])

HSP 1 Score: 827.4 bits (2136), Expect = 5.2e-236
Identity = 387/393 (98.47%), Postives = 391/393 (99.49%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIG+TGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGITGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENC+VNG
Sbjct: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCDVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIID+P+ RHDPPFTEDMPRLDSPNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPSHRHDPPFTEDMPRLDSPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK
Sbjct: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IEDYGFEEGERLPLGELMKGKN VWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS
Sbjct: 301 IEDYGFEEGERLPLGELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393

[Close]

BLAST of CmoCh01G018570 vs. NCBI nr
Match: XP_023523285.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 824.3 bits (2128), Expect = 4.4e-235
Identity = 388/393 (98.73%), Postives = 390/393 (99.24%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRPNWNADHPIEYIQCDVSD EDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG
Sbjct: 61  VARRPRPNWNADHPIEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIID+PTQRHDPPFTEDMPRLD+PNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK
Sbjct: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IEDYGFEEGERLPL ELMKGKN VWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS
Sbjct: 301 IEDYGFEEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393

[Close]

BLAST of CmoCh01G018570 vs. NCBI nr
Match: AJE29749.1 (putative progesterone 5-beta-reductase [Cucurbita pepo])

HSP 1 Score: 818.5 bits (2113), Expect = 2.4e-233
Identity = 386/393 (98.22%), Postives = 388/393 (98.73%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           M WWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MRWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRPNWNADH IEYIQCDVSD EDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG
Sbjct: 61  VARRPRPNWNADHLIEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIID+PTQRHDPPFTEDMPRLD+PNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK
Sbjct: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IEDYGFEEGERLPL ELMKGKN VWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS
Sbjct: 301 IEDYGFEEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393

[Close]

BLAST of CmoCh01G018570 vs. NCBI nr
Match: XP_038899926.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase [Benincasa hispida])

HSP 1 Score: 748.4 bits (1931), Expect = 3.1e-212
Identity = 344/393 (87.53%), Postives = 367/393 (93.38%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDE PRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           +ARRPRP WNADHPIEYIQCD+SDPEDAEAKLS+LTDVTH+FYVTWTN PTEIENCE N 
Sbjct: 61  IARRPRPTWNADHPIEYIQCDISDPEDAEAKLSQLTDVTHLFYVTWTNRPTEIENCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHY+GS ++I+D   Q HDPPFTED+PRL+ PNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYLGSLQSIVDKSVQPHDPPFTEDLPRLEGPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YY+QEDLLFEEIE+SQKKDLTWAVIRPN IFGFSPFSL+NIVGTLC YAAICKHEGR LK
Sbjct: 181 YYSQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLINIVGTLCVYAAICKHEGRTLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPG+KSAWE + V SDADLIAEQHIWTAVDPYAKNEAFNC+NGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGSKSAWEDFQVGSDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IEDYGFE+GERL L ELMK K+ VW+EIVKENQLQPTKLE++ NWW TD+  S SGNIDS
Sbjct: 301 IEDYGFEDGERLTLVELMKDKSEVWDEIVKENQLQPTKLEEITNWWFTDVIFSLSGNIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKEHGFLGFRNSKNSFISWIDK+KAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393

[Close]

BLAST of CmoCh01G018570 vs. TAIR 10
Match: AT4G24220.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 652.9 bits (1683), Expect = 1.6e-187
Identity = 303/393 (77.10%), Postives = 334/393 (84.99%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKK +EDE  +SFESVAL+IGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKLDEDEPSQSFESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP WNADHPI+YIQCDVSD ED  +KLS LTDVTHVFYVTWTN  +E ENCE NG
Sbjct: 61  VARRPRPTWNADHPIDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNRESESENCEANG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
            MLRNVL+++IP APNLRH+CLQTGTKHY+G F N +D P  RHDPPFTEDMPRL   NF
Sbjct: 121 SMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPFTN-VDGP--RHDPPFTEDMPRLQIQNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQED+LFEEI+K +   +TW++ RPN IFGFSP+SLMNIVGTLC YAAICKHEG PL 
Sbjct: 181 YYTQEDILFEEIKKIE--TVTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLL 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPG+K AWEG+   SDADLIAEQ IW AVDPYAKNEAFNC+N D+FKWKH WK+LAEQF 
Sbjct: 241 FPGSKKAWEGFMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKILAEQFG 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IE+YGFEEG+ L L E+MKGK  VWEE+VKENQLQ  KLE+V  WW  D+ L   G IDS
Sbjct: 301 IEEYGFEEGKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEGMIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKE+GFLGFRNS NSFISWIDK KAFKIVP
Sbjct: 361 MNKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 388

[Close]

BLAST of CmoCh01G018570 vs. TAIR 10
Match: AT4G24220.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 646.4 bits (1666), Expect = 1.5e-185
Identity = 302/393 (76.84%), Postives = 333/393 (84.73%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAA KK +EDE  +SFESVAL+IGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAA-KKLDEDEPSQSFESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP WNADHPI+YIQCDVSD ED  +KLS LTDVTHVFYVTWTN  +E ENCE NG
Sbjct: 61  VARRPRPTWNADHPIDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNRESESENCEANG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
            MLRNVL+++IP APNLRH+CLQTGTKHY+G F N +D P  RHDPPFTEDMPRL   NF
Sbjct: 121 SMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPFTN-VDGP--RHDPPFTEDMPRLQIQNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQED+LFEEI+K +   +TW++ RPN IFGFSP+SLMNIVGTLC YAAICKHEG PL 
Sbjct: 181 YYTQEDILFEEIKKIE--TVTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLL 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPG+K AWEG+   SDADLIAEQ IW AVDPYAKNEAFNC+N D+FKWKH WK+LAEQF 
Sbjct: 241 FPGSKKAWEGFMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKILAEQFG 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IE+YGFEEG+ L L E+MKGK  VWEE+VKENQLQ  KLE+V  WW  D+ L   G IDS
Sbjct: 301 IEEYGFEEGKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEGMIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKE+GFLGFRNS NSFISWIDK KAFKIVP
Sbjct: 361 MNKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 387

[Close]

BLAST of CmoCh01G018570 vs. TAIR 10
Match: AT5G58750.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 237.7 bits (605), Expect = 1.6e-62
Identity = 142/379 (37.47%), Postives = 206/379 (54.35%), Query Frame = 0


Query: 26  ESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRPRPNWNADHPIEYIQCDVSDP 85
           E+VAL+ GVTG+VG  + + L L   PG  W++YGVAR P  N +      +I CD+ + 
Sbjct: 16  ENVALIFGVTGLVGREIVKTL-LMSKPG--WRIYGVARNPEIN-SMTKMYNFISCDLLNA 75

Query: 86  EDAEAKLSKLTD-VTHVFYVTWT-NLPTEIENCEVNGK-MLRNVLRSVIPNAPNLRHICL 145
            + + +LS L D V+HVF+VTW+   P + + C V  K ML N L +++PNA  L+H  L
Sbjct: 76  SETKQRLSPLQDIVSHVFWVTWSGEFPLDTDECCVQNKTMLMNALDAILPNAKRLKHFSL 135

Query: 146 QTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDS-PNFYYTQEDLLFEEIEKSQKKDLT 205
           QTG KHY+   E  +          ++E+ PR  S  NFYY  EDLL E+I +S    + 
Sbjct: 136 QTGMKHYVSLVEETMARGEGSSLYYYSEECPRKSSGKNFYYVLEDLLKEKITRS---SVV 195

Query: 206 WAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLKFPGTKSAW-EGYSVVSDADLI 265
           W+V RP  + G S  +L N +G+LC Y A+CK+   P  F GT+  W E Y   SD++L+
Sbjct: 196 WSVQRPGLLMGSSSRTLYNFMGSLCVYGAMCKYLNLPFVFGGTRECWEESYIDGSDSNLV 255

Query: 266 AEQHIWTAVDPYA--KNEAFNCSNGDVFKWKHFWKVLAEQFNI---EDYGFEEGERLPLG 325
           AEQHI+ A       K EAFN  NG  F WK  W  + ++  +   E   F+EG     G
Sbjct: 256 AEQHIFAATSGKVREKGEAFNAINGVGFTWKEIWPEIGKKLGVQVNETTMFDEG--FWFG 315

Query: 326 ELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGN-IDSMNKSKEHGFLGFRN 385
             M  +  VW+EIV + +L  T++ED+ANW+  D         +    K    GF     
Sbjct: 316 REMVERKHVWDEIVVKEKLVRTEIEDLANWYFLDALFRCPFKLLGKREKVDRFGFKRKYR 375

Query: 386 SKNSFISWIDKVKAFKIVP 394
           + +S + WID ++  K++P
Sbjct: 376 TLDSVLYWIDVMRDEKLIP 385

The following BLAST results are available for this feature:

BLAST of CmoCh01G018570 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9STX2	2.3e-186	77.10	3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP...	[more]
A0A221J5W8	2.1e-179	74.05	(S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta cataria OX=39347 GN=ISY1 PE=1 ...	[more]
A0A221J5X3	4.6e-179	74.05	(S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta racemosa OX=54731 GN=ISY1 PE=1...	[more]
A0A1C9CX66	1.5e-169	71.83	(S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC...	[more]
Q6PQJ9	7.4e-169	70.23	3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata OX=49450 PE=1 SV=1	[more]

BLAST of CmoCh01G018570 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FQC1	3.2e-239	100.00	3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1J0N7	2.5e-236	98.47	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A0B5CYJ8	1.2e-233	98.22	Putative progesterone 5-beta-reductase OS=Cucurbita pepo OX=3663 PE=2 SV=1	[more]
A0A5A7T3B3	3.4e-209	85.75	3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A1S3BHA7	3.4e-209	85.75	3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo OX=3656 GN=LOC10348983...	[more]

BLAST of CmoCh01G018570 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022940360.1	6.6e-239	100.00	3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucurbita moschata] >KAG6608438.1 3-o...	[more]
XP_022981805.1	5.2e-236	98.47	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita maxima]	[more]
XP_023523285.1	4.4e-235	98.73	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita pepo subsp. pepo]	[more]
AJE29749.1	2.4e-233	98.22	putative progesterone 5-beta-reductase [Cucurbita pepo]	[more]
XP_038899926.1	3.1e-212	87.53	3-oxo-Delta(4,5)-steroid 5-beta-reductase [Benincasa hispida]	[more]

BLAST of CmoCh01G018570 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G24220.1	1.6e-187	77.10	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G24220.2	1.5e-185	76.84	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G58750.1	1.6e-62	37.47	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001509	NAD-dependent epimerase/dehydratase	PFAM	PF01370	Epimerase	coord: 29..256 e-value: 6.9E-5 score: 22.4
None	No IPR available	GENE3D	3.40.50.720		coord: 25..393 e-value: 9.0E-141 score: 471.0
None	No IPR available	PANTHER	PTHR32487:SF20	3-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE-LIKE	coord: 1..393
None	No IPR available	PANTHER	PTHR32487	3-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE	coord: 1..393
None	No IPR available	CDD	cd08948	5beta-POR_like_SDR_a	coord: 28..337 e-value: 7.99921E-139 score: 396.231
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 28..302

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh01G018570.1	CmoCh01G018570.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009611	response to wounding
biological_process	GO:0008202	steroid metabolic process
biological_process	GO:0010051	xylem and phloem pattern formation
molecular_function	GO:0047568	3-oxo-5-beta-steroid 4-dehydrogenase activity
molecular_function	GO:0035671	enone reductase activity
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0003824	catalytic activity