CmoCh01G008890 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh01G008890
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	pectinesterase inhibitor-like
Location	Cmo_Chr01: 4863766 .. 4864305 (-)
RNA-Seq Expression	CmoCh01G008890
Synteny	CmoCh01G008890

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACATCTTCTAGTTTTCTTGGAGTCTTTAGGATTGTCCTTTTAGTACTGTTCTTCAATGGGATGGTGCCCATGCATGCGGCTTCCCAAGACGACATCGTTTCCACCATCTGCAAGAAAACTAGAAACCCTTCTTTTTGCTTTAACGTGTTGAAATCTGCTGGCACCACAGACTTAAAAGGGCTGGCCACCTTCACCCTCAACCTCGCCCACGACAAGGTTGGTCAAACCCGTGCCCTCGCCCTGTCCCTGGCATCCAAGGCAGCCGATCCCAAGCTTAAGGAGCGATATGCCACCTGTGCTGAACAGTACGACGATGCCGCCGACGACATCGAGGATGGGAAGAACGACCTGGGGGAAGGTGACTACAATGGCGTCAACATTAAGGCGTCTGCAGCCATGACAGAGGCCGGCGACTGTCTGGACAGCTTCACGCAGCCGCCGAAGGACCCATCGGCGCTGTCTGGCAACGGGAAGACTGTGGAAGATATTTGTAGTATCATCTTGGTTATAGCCAATCTTCTCCTTGGGCGTGACTAA

mRNA sequence

ATGACATCTTCTAGTTTTCTTGGAGTCTTTAGGATTGTCCTTTTAGTACTGTTCTTCAATGGGATGGTGCCCATGCATGCGGCTTCCCAAGACGACATCGTTTCCACCATCTGCAAGAAAACTAGAAACCCTTCTTTTTGCTTTAACGTGTTGAAATCTGCTGGCACCACAGACTTAAAAGGGCTGGCCACCTTCACCCTCAACCTCGCCCACGACAAGGTTGGTCAAACCCGTGCCCTCGCCCTGTCCCTGGCATCCAAGGCAGCCGATCCCAAGCTTAAGGAGCGATATGCCACCTGTGCTGAACAGTACGACGATGCCGCCGACGACATCGAGGATGGGAAGAACGACCTGGGGGAAGGTGACTACAATGGCGTCAACATTAAGGCGTCTGCAGCCATGACAGAGGCCGGCGACTGTCTGGACAGCTTCACGCAGCCGCCGAAGGACCCATCGGCGCTGTCTGGCAACGGGAAGACTGTGGAAGATATTTGTAGTATCATCTTGGTTATAGCCAATCTTCTCCTTGGGCGTGACTAA

Coding sequence (CDS)

ATGACATCTTCTAGTTTTCTTGGAGTCTTTAGGATTGTCCTTTTAGTACTGTTCTTCAATGGGATGGTGCCCATGCATGCGGCTTCCCAAGACGACATCGTTTCCACCATCTGCAAGAAAACTAGAAACCCTTCTTTTTGCTTTAACGTGTTGAAATCTGCTGGCACCACAGACTTAAAAGGGCTGGCCACCTTCACCCTCAACCTCGCCCACGACAAGGTTGGTCAAACCCGTGCCCTCGCCCTGTCCCTGGCATCCAAGGCAGCCGATCCCAAGCTTAAGGAGCGATATGCCACCTGTGCTGAACAGTACGACGATGCCGCCGACGACATCGAGGATGGGAAGAACGACCTGGGGGAAGGTGACTACAATGGCGTCAACATTAAGGCGTCTGCAGCCATGACAGAGGCCGGCGACTGTCTGGACAGCTTCACGCAGCCGCCGAAGGACCCATCGGCGCTGTCTGGCAACGGGAAGACTGTGGAAGATATTTGTAGTATCATCTTGGTTATAGCCAATCTTCTCCTTGGGCGTGACTAA

Protein sequence

MTSSSFLGVFRIVLLVLFFNGMVPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLKGLATFTLNLAHDKVGQTRALALSLASKAADPKLKERYATCAEQYDDAADDIEDGKNDLGEGDYNGVNIKASAAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGRD

Homology

[Close]

BLAST of CmoCh01G008890 vs. ExPASy Swiss-Prot
Match: P83326 (Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2)

HSP 1 Score: 133.3 bits (334), Expect = 2.8e-30
Identity = 74/170 (43.53%), Postives = 104/170 (61.18%), Query Frame = 0


Query: 12  IVLLVLFFNGMVPMHAA-SQDDIVSTICKKTRNPSFCFNVLKS---AGTTDLKGLATFTL 71
           ++L++LF + +    +  +++ ++S IC KTRNPS C   L+S   + + DLKGL  F++
Sbjct: 14  LLLVILFISPLSQRPSVKAENHLISEICPKTRNPSLCLQALESDPRSASKDLKGLGQFSI 73

Query: 72  NLAHDKVGQTRALALSLASKAADPKLKERYATCAEQYDDAADDIEDGKNDLGEGDYNGVN 131
           ++A     QT  +  SL ++A DPKLK RY TC+E Y DA D +   K  L  GDYN +N
Sbjct: 74  DIAQASAKQTSKIIASLTNQATDPKLKGRYETCSENYADAIDSLGQAKQFLTSGDYNSLN 133

Query: 132 IKASAAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLG 178
           I ASAA   AG C DSF  PP  P+ L      +ED+C I+LVI+NLL G
Sbjct: 134 IYASAAFDGAGTCEDSFEGPPNIPTQLHQADLKLEDLCDIVLVISNLLPG 183

[Close]

BLAST of CmoCh01G008890 vs. ExPASy Swiss-Prot
Match: Q9LNF2 (Pectinesterase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PMEI1 PE=1 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 9.3e-18
Identity = 52/143 (36.36%), Postives = 80/143 (55.94%), Query Frame = 0


Query: 34  VSTICKKTRNPSFCFNVLKSA-GTTDLKGLATFTLNLAHDKVGQTRALALSLASKAADPK 93
           +STIC KT NPSFC   L +   + +L+ LA  TL+    +  QT     S+     DP+
Sbjct: 31  MSTICDKTLNPSFCLKFLNTKFASPNLQALAKTTLDSTQARATQTLKKLQSIIDGGVDPR 90

Query: 94  LKERYATCAEQYDDAADDIEDGKNDLGEGDYNGVNIKASAAMTEAGDCLDSFTQPPKDPS 153
            K  Y +C ++Y+ A  ++E+    L  GD  G+N+K SAA+  A  CLD   +     S
Sbjct: 91  SKLAYRSCVDEYESAIGNLEEAFEHLASGDGMGMNMKVSAALDGADTCLDDVKRLRSVDS 150

Query: 154 ALSGNGKTVEDICSIILVIANLL 176
           ++  N KT++++C I LVI+N+L
Sbjct: 151 SVVNNSKTIKNLCGIALVISNML 173

[Close]

BLAST of CmoCh01G008890 vs. ExPASy Swiss-Prot
Match: Q9LUV1 (Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 3.0e-16
Identity = 58/168 (34.52%), Postives = 81/168 (48.21%), Query Frame = 0


Query: 11  RIVLLVLFFNGMVPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTT---DLKGLATFTL 70
           R+++  L F  M     A   DI   IC K +N SFC + +KS   T   DL+ LA  T 
Sbjct: 8   RVLMSSLMFFVMTGSLNAQVADI-KAICGKAKNQSFCTSYMKSNPKTSGADLQTLANITF 67

Query: 71  NLAHDKVGQTRALALSLASKAADPKLKERYATCAEQYDDAADDIEDGKNDLGEGDYNGVN 130
             A     +      SL   A +P +K+ Y +C + Y  A   + D K  L  GD  G+N
Sbjct: 68  GSAQTSASEGFRKIQSLVKTATNPTMKKAYTSCVQHYKSAISSLNDAKQSLASGDGKGLN 127

Query: 131 IKASAAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLL 176
           IK SAAM     C         DPSA+  +G   ++IC I+LVI+N++
Sbjct: 128 IKVSAAMEGPSTCEQDMADFKVDPSAVKNSG-DFQNICGIVLVISNMM 173

[Close]

BLAST of CmoCh01G008890 vs. ExPASy Swiss-Prot
Match: Q0J8J8 (Pectinesterase inhibitor 28 OS=Oryza sativa subsp. japonica OX=39947 GN=PMEI28 PE=2 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 1.0e-03
Identity = 39/156 (25.00%), Postives = 67/156 (42.95%), Query Frame = 0


Query: 33  IVSTICKKTRNPSFCFNVL---KSAGTTDLKGLATFTLNLAHDKVGQTRALALSL----- 92
           +V   C  T     C + L    S+ T D++GL+   +++A          A +L     
Sbjct: 61  LVRATCNSTAYYDVCVSALAADPSSTTADVRGLSAIAVSVAAANASGAAQAAAALANGTA 120

Query: 93  ---ASKAADPKLKERYATCAEQYDDAADDIEDGKNDLGEGDYN--GVNIKASAAMTEAGD 152
              A+ A D  ++     CA +Y DA D +   K  +G+ DY+   V++ A A   +   
Sbjct: 121 PLAAAAAGDGTVQALLRACAGKYGDARDALAAAKESMGQQDYDLATVHVSAGAEYPQVCK 180

Query: 153 CLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLL 176
            L    +P   P+ L+   + +  +CS+ L I  LL
Sbjct: 181 ALFRRQRPGAYPAELAAREEALNKLCSVALDIIALL 216

[Close]

BLAST of CmoCh01G008890 vs. ExPASy TrEMBL
Match: A0A6J1F4D5 (pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111440166 PE=4 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 2.3e-88
Identity = 172/178 (96.63%), Postives = 173/178 (97.19%), Query Frame = 0


Query: 1   MTSSSFLGVFRIVLLVLFFNGMVPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60
           M SSSFL VFRIVLLVLFFNGMVPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK
Sbjct: 1   MASSSFLAVFRIVLLVLFFNGMVPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60

Query: 61  GLATFTLNLAHDKVGQTRALALSLASKAADPKLKERYATCAEQYDDAADDIEDGKNDLGE 120
           GLATFTLNLAHDKV QT ALALSLASKAADPKLK+RYATCAEQYDDAADDIEDGKNDL E
Sbjct: 61  GLATFTLNLAHDKVAQTHALALSLASKAADPKLKDRYATCAEQYDDAADDIEDGKNDLRE 120

Query: 121 GDYNGVNIKASAAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGR 179
           GDYNGVNIKASAAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGR
Sbjct: 121 GDYNGVNIKASAAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGR 178

[Close]

BLAST of CmoCh01G008890 vs. ExPASy TrEMBL
Match: A0A6J1EZI7 (pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111440672 PE=4 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 3.0e-88
Identity = 171/178 (96.07%), Postives = 171/178 (96.07%), Query Frame = 0


Query: 1   MTSSSFLGVFRIVLLVLFFNGMVPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60
           M SSSFL VFRIVLLVLFFNGM PMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK
Sbjct: 1   MASSSFLAVFRIVLLVLFFNGMAPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60

Query: 61  GLATFTLNLAHDKVGQTRALALSLASKAADPKLKERYATCAEQYDDAADDIEDGKNDLGE 120
           GLATFTLNLAHDKV QTRALALSLASK  DPKLKERYATCAEQYDDAADDIEDGKNDLGE
Sbjct: 61  GLATFTLNLAHDKVAQTRALALSLASKETDPKLKERYATCAEQYDDAADDIEDGKNDLGE 120

Query: 121 GDYNGVNIKASAAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGR 179
           GDYNGVNIKASAAMTE GDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGR
Sbjct: 121 GDYNGVNIKASAAMTEVGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGR 178

[Close]

BLAST of CmoCh01G008890 vs. ExPASy TrEMBL
Match: A0A6J1F469 (pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111440164 PE=4 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 6.8e-88
Identity = 172/178 (96.63%), Postives = 172/178 (96.63%), Query Frame = 0


Query: 1   MTSSSFLGVFRIVLLVLFFNGMVPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60
           M SSSFL VFRIVLLVLFFNGMVPMHAASQDDIVSTICKKTRN SFCFNVLKSAGTTDLK
Sbjct: 1   MASSSFLAVFRIVLLVLFFNGMVPMHAASQDDIVSTICKKTRNSSFCFNVLKSAGTTDLK 60

Query: 61  GLATFTLNLAHDKVGQTRALALSLASKAADPKLKERYATCAEQYDDAADDIEDGKNDLGE 120
           GLATFTLNLAHDKV QTRALALSLASKA DPKLKERYATCAEQYDDAADDIEDGKNDLGE
Sbjct: 61  GLATFTLNLAHDKVAQTRALALSLASKATDPKLKERYATCAEQYDDAADDIEDGKNDLGE 120

Query: 121 GDYNGVNIKASAAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGR 179
           GDYNGVNIKASAAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLL R
Sbjct: 121 GDYNGVNIKASAAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLRR 178

[Close]

BLAST of CmoCh01G008890 vs. ExPASy TrEMBL
Match: A0A6J1F473 (pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111440170 PE=4 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 3.4e-87
Identity = 169/178 (94.94%), Postives = 170/178 (95.51%), Query Frame = 0


Query: 1   MTSSSFLGVFRIVLLVLFFNGMVPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60
           M SSSFL VFRIVLLV+FFNGM PMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK
Sbjct: 1   MASSSFLAVFRIVLLVMFFNGMAPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60

Query: 61  GLATFTLNLAHDKVGQTRALALSLASKAADPKLKERYATCAEQYDDAADDIEDGKNDLGE 120
           GLATFTLNLAHDKV QTRALALSLASKA DPKLKERYATCAEQYDDA DDIEDGKNDLGE
Sbjct: 61  GLATFTLNLAHDKVAQTRALALSLASKATDPKLKERYATCAEQYDDADDDIEDGKNDLGE 120

Query: 121 GDYNGVNIKASAAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGR 179
           GDYNGVNIKASAAMTE GDCLDSFTQPPKDPSALS NGKTVEDICSIILVIANLLLGR
Sbjct: 121 GDYNGVNIKASAAMTEVGDCLDSFTQPPKDPSALSSNGKTVEDICSIILVIANLLLGR 178

[Close]

BLAST of CmoCh01G008890 vs. ExPASy TrEMBL
Match: A0A6J1EZ77 (pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111440171 PE=4 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 9.8e-87
Identity = 168/178 (94.38%), Postives = 169/178 (94.94%), Query Frame = 0


Query: 1   MTSSSFLGVFRIVLLVLFFNGMVPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60
           M SSSFL VFRIVLLV+FFNGM PMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK
Sbjct: 1   MASSSFLAVFRIVLLVMFFNGMAPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60

Query: 61  GLATFTLNLAHDKVGQTRALALSLASKAADPKLKERYATCAEQYDDAADDIEDGKNDLGE 120
           GLATFTLNLAHDKV QTRALALSL SKA DPKLKERYATCAEQYDDAADDIEDGKNDLGE
Sbjct: 61  GLATFTLNLAHDKVAQTRALALSLVSKATDPKLKERYATCAEQYDDAADDIEDGKNDLGE 120

Query: 121 GDYNGVNIKASAAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGR 179
           GDYNGVNIKASAAMTE GDCLDS TQPPKDPSALS NGKTVEDICSIILVIANLLLGR
Sbjct: 121 GDYNGVNIKASAAMTEVGDCLDSITQPPKDPSALSSNGKTVEDICSIILVIANLLLGR 178

[Close]

BLAST of CmoCh01G008890 vs. NCBI nr
Match: XP_023522250.1 (pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 335.5 bits (859), Expect = 2.8e-88
Identity = 173/178 (97.19%), Postives = 173/178 (97.19%), Query Frame = 0


Query: 1   MTSSSFLGVFRIVLLVLFFNGMVPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60
           M SSSFL VFRIVLLVLFFNGMVPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGT DLK
Sbjct: 1   MASSSFLAVFRIVLLVLFFNGMVPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTRDLK 60

Query: 61  GLATFTLNLAHDKVGQTRALALSLASKAADPKLKERYATCAEQYDDAADDIEDGKNDLGE 120
           GLATFTLNLAHDKV QTRALALSLASKAADPKLKERYATCAEQYDDAADDIEDGKNDL E
Sbjct: 61  GLATFTLNLAHDKVAQTRALALSLASKAADPKLKERYATCAEQYDDAADDIEDGKNDLRE 120

Query: 121 GDYNGVNIKASAAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGR 179
           GDYNGVNIKASAAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGR
Sbjct: 121 GDYNGVNIKASAAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGR 178

[Close]

BLAST of CmoCh01G008890 vs. NCBI nr
Match: XP_022933240.1 (pectinesterase inhibitor-like [Cucurbita moschata])

HSP 1 Score: 334.7 bits (857), Expect = 4.8e-88
Identity = 172/178 (96.63%), Postives = 173/178 (97.19%), Query Frame = 0


Query: 1   MTSSSFLGVFRIVLLVLFFNGMVPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60
           M SSSFL VFRIVLLVLFFNGMVPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK
Sbjct: 1   MASSSFLAVFRIVLLVLFFNGMVPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60

Query: 61  GLATFTLNLAHDKVGQTRALALSLASKAADPKLKERYATCAEQYDDAADDIEDGKNDLGE 120
           GLATFTLNLAHDKV QT ALALSLASKAADPKLK+RYATCAEQYDDAADDIEDGKNDL E
Sbjct: 61  GLATFTLNLAHDKVAQTHALALSLASKAADPKLKDRYATCAEQYDDAADDIEDGKNDLRE 120

Query: 121 GDYNGVNIKASAAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGR 179
           GDYNGVNIKASAAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGR
Sbjct: 121 GDYNGVNIKASAAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGR 178

[Close]

BLAST of CmoCh01G008890 vs. NCBI nr
Match: XP_022933241.1 (pectinesterase inhibitor-like [Cucurbita moschata] >XP_022933352.1 pectinesterase inhibitor-like [Cucurbita moschata])

HSP 1 Score: 334.3 bits (856), Expect = 6.3e-88
Identity = 171/178 (96.07%), Postives = 171/178 (96.07%), Query Frame = 0


Query: 1   MTSSSFLGVFRIVLLVLFFNGMVPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60
           M SSSFL VFRIVLLVLFFNGM PMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK
Sbjct: 1   MASSSFLAVFRIVLLVLFFNGMAPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60

Query: 61  GLATFTLNLAHDKVGQTRALALSLASKAADPKLKERYATCAEQYDDAADDIEDGKNDLGE 120
           GLATFTLNLAHDKV QTRALALSLASK  DPKLKERYATCAEQYDDAADDIEDGKNDLGE
Sbjct: 61  GLATFTLNLAHDKVAQTRALALSLASKETDPKLKERYATCAEQYDDAADDIEDGKNDLGE 120

Query: 121 GDYNGVNIKASAAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGR 179
           GDYNGVNIKASAAMTE GDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGR
Sbjct: 121 GDYNGVNIKASAAMTEVGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGR 178

[Close]

BLAST of CmoCh01G008890 vs. NCBI nr
Match: XP_022933238.1 (pectinesterase inhibitor-like [Cucurbita moschata])

HSP 1 Score: 333.2 bits (853), Expect = 1.4e-87
Identity = 172/178 (96.63%), Postives = 172/178 (96.63%), Query Frame = 0


Query: 1   MTSSSFLGVFRIVLLVLFFNGMVPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60
           M SSSFL VFRIVLLVLFFNGMVPMHAASQDDIVSTICKKTRN SFCFNVLKSAGTTDLK
Sbjct: 1   MASSSFLAVFRIVLLVLFFNGMVPMHAASQDDIVSTICKKTRNSSFCFNVLKSAGTTDLK 60

Query: 61  GLATFTLNLAHDKVGQTRALALSLASKAADPKLKERYATCAEQYDDAADDIEDGKNDLGE 120
           GLATFTLNLAHDKV QTRALALSLASKA DPKLKERYATCAEQYDDAADDIEDGKNDLGE
Sbjct: 61  GLATFTLNLAHDKVAQTRALALSLASKATDPKLKERYATCAEQYDDAADDIEDGKNDLGE 120

Query: 121 GDYNGVNIKASAAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGR 179
           GDYNGVNIKASAAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLL R
Sbjct: 121 GDYNGVNIKASAAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLRR 178

[Close]

BLAST of CmoCh01G008890 vs. NCBI nr
Match: XP_022933243.1 (pectinesterase inhibitor-like [Cucurbita moschata])

HSP 1 Score: 330.9 bits (847), Expect = 7.0e-87
Identity = 169/178 (94.94%), Postives = 170/178 (95.51%), Query Frame = 0


Query: 1   MTSSSFLGVFRIVLLVLFFNGMVPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60
           M SSSFL VFRIVLLV+FFNGM PMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK
Sbjct: 1   MASSSFLAVFRIVLLVMFFNGMAPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTTDLK 60

Query: 61  GLATFTLNLAHDKVGQTRALALSLASKAADPKLKERYATCAEQYDDAADDIEDGKNDLGE 120
           GLATFTLNLAHDKV QTRALALSLASKA DPKLKERYATCAEQYDDA DDIEDGKNDLGE
Sbjct: 61  GLATFTLNLAHDKVAQTRALALSLASKATDPKLKERYATCAEQYDDADDDIEDGKNDLGE 120

Query: 121 GDYNGVNIKASAAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLGR 179
           GDYNGVNIKASAAMTE GDCLDSFTQPPKDPSALS NGKTVEDICSIILVIANLLLGR
Sbjct: 121 GDYNGVNIKASAAMTEVGDCLDSFTQPPKDPSALSSNGKTVEDICSIILVIANLLLGR 178

[Close]

BLAST of CmoCh01G008890 vs. TAIR 10
Match: AT1G48020.1 (pectin methylesterase inhibitor 1 )

HSP 1 Score: 91.7 bits (226), Expect = 6.6e-19
Identity = 52/143 (36.36%), Postives = 80/143 (55.94%), Query Frame = 0


Query: 34  VSTICKKTRNPSFCFNVLKSA-GTTDLKGLATFTLNLAHDKVGQTRALALSLASKAADPK 93
           +STIC KT NPSFC   L +   + +L+ LA  TL+    +  QT     S+     DP+
Sbjct: 31  MSTICDKTLNPSFCLKFLNTKFASPNLQALAKTTLDSTQARATQTLKKLQSIIDGGVDPR 90

Query: 94  LKERYATCAEQYDDAADDIEDGKNDLGEGDYNGVNIKASAAMTEAGDCLDSFTQPPKDPS 153
            K  Y +C ++Y+ A  ++E+    L  GD  G+N+K SAA+  A  CLD   +     S
Sbjct: 91  SKLAYRSCVDEYESAIGNLEEAFEHLASGDGMGMNMKVSAALDGADTCLDDVKRLRSVDS 150

Query: 154 ALSGNGKTVEDICSIILVIANLL 176
           ++  N KT++++C I LVI+N+L
Sbjct: 151 SVVNNSKTIKNLCGIALVISNML 173

[Close]

BLAST of CmoCh01G008890 vs. TAIR 10
Match: AT3G17220.1 (pectin methylesterase inhibitor 2 )

HSP 1 Score: 86.7 bits (213), Expect = 2.1e-17
Identity = 58/168 (34.52%), Postives = 81/168 (48.21%), Query Frame = 0


Query: 11  RIVLLVLFFNGMVPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTT---DLKGLATFTL 70
           R+++  L F  M     A   DI   IC K +N SFC + +KS   T   DL+ LA  T 
Sbjct: 8   RVLMSSLMFFVMTGSLNAQVADI-KAICGKAKNQSFCTSYMKSNPKTSGADLQTLANITF 67

Query: 71  NLAHDKVGQTRALALSLASKAADPKLKERYATCAEQYDDAADDIEDGKNDLGEGDYNGVN 130
             A     +      SL   A +P +K+ Y +C + Y  A   + D K  L  GD  G+N
Sbjct: 68  GSAQTSASEGFRKIQSLVKTATNPTMKKAYTSCVQHYKSAISSLNDAKQSLASGDGKGLN 127

Query: 131 IKASAAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLL 176
           IK SAAM     C         DPSA+  +G   ++IC I+LVI+N++
Sbjct: 128 IKVSAAMEGPSTCEQDMADFKVDPSAVKNSG-DFQNICGIVLVISNMM 173

[Close]

BLAST of CmoCh01G008890 vs. TAIR 10
Match: AT3G36659.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 50.4 bits (119), Expect = 1.7e-06
Identity = 42/142 (29.58%), Postives = 62/142 (43.66%), Query Frame = 0


Query: 34  VSTICKKTRNPSFCFNVLKSAGTTDLKGLATFTLNLAHDKVGQTRALALSLASKAADPKL 93
           + TIC KT NP  C + +    T  LK   +  L LA          A+++  K      
Sbjct: 130 IKTICGKTDNPPLCESSVSPLLTPQLKPNTSSVLILAIQASITATKAAMAIVEKV----- 189

Query: 94  KERYATCAEQYDDAADDIEDGKNDLGEGDYNGVNIKASAAMTEAGDCLDSFTQPPKDPSA 153
               + C E YDDA  ++ED  N +   D   VN   SAAMT+   C D F +   +P+ 
Sbjct: 190 --DASDCQELYDDAVVNLEDAVNAVKSSDIATVNTNLSAAMTDYSTCNDGF-EESGEPNP 249

Query: 154 LSGNGKTVEDICSIILVIANLL 176
           L+     +  + S  L I+ L+
Sbjct: 250 LAYVADKLTKMVSNCLAISTLI 263

[Close]

BLAST of CmoCh01G008890 vs. TAIR 10
Match: AT2G47340.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 48.1 bits (113), Expect = 8.4e-06
Identity = 25/86 (29.07%), Postives = 43/86 (50.00%), Query Frame = 0


Query: 93  LKERYATCAEQYDDAADDIEDGKNDLGEGDYNGVNIKASAAMTEAGDCLDSFTQPPKDPS 152
           +KE  + C E YD     +ED K  + EGDY  ++   SAA+++   C D+F   P   S
Sbjct: 197 VKEAMSVCVENYDSLISGLEDAKMAMEEGDYGRLDSVLSAAISDVSTCSDNFVDIPDVDS 256

Query: 153 ALSGNGKTVEDICSIILVIANLLLGR 179
             +     ++ +CS +L ++  +  R
Sbjct: 257 PTASLDDLMKKLCSNVLAMSQKVQNR 282

[Close]

BLAST of CmoCh01G008890 vs. TAIR 10
Match: AT3G17152.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 43.1 bits (100), Expect = 2.7e-04
Identity = 40/147 (27.21%), Postives = 61/147 (41.50%), Query Frame = 0


Query: 33  IVSTICKKTRNPSFC---FNVLKSAGTTDLKGLATFTLNLAHDKVGQTRALALSLASKAA 92
           +V  +CK+ R  + C    NV   + T++L+GLA+ +L+    K   T    +S+     
Sbjct: 33  LVQRLCKRNRYQALCISTLNVDPRSKTSNLQGLASISLDATTKKFNVTLTYYISVLKNVR 92

Query: 93  DPKLKERYATCAEQYDDAADD-IEDGKNDLGEGDYNGVNIKASAAMTEAGDCLDSFTQPP 152
                ERY TC E+Y  A D  +   K DL    Y     +    + + G C D F    
Sbjct: 93  GRVDFERYGTCIEEYGAAVDRFLPAVKADLKAKKYPEAMSEMKDVVAKPGYCEDQFA--- 152

Query: 153 KDPSALSGNGKTVEDICSIILVIANLL 176
              S ++   K V DI  +   I   L
Sbjct: 153 -GESPVTARNKAVHDIADMTADIIKTL 175

The following BLAST results are available for this feature:

BLAST of CmoCh01G008890 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P83326	2.8e-30	43.53	Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2	[more]
Q9LNF2	9.3e-18	36.36	Pectinesterase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PMEI1 PE=1 SV=1	[more]
Q9LUV1	3.0e-16	34.52	Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1	[more]
Q0J8J8	1.0e-03	25.00	Pectinesterase inhibitor 28 OS=Oryza sativa subsp. japonica OX=39947 GN=PMEI28 P...	[more]

BLAST of CmoCh01G008890 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F4D5	2.3e-88	96.63	pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111440166 PE=4...	[more]
A0A6J1EZI7	3.0e-88	96.07	pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111440672 PE=4...	[more]
A0A6J1F469	6.8e-88	96.63	pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111440164 PE=4...	[more]
A0A6J1F473	3.4e-87	94.94	pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111440170 PE=4...	[more]
A0A6J1EZ77	9.8e-87	94.38	pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111440171 PE=4...	[more]

BLAST of CmoCh01G008890 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023522250.1	2.8e-88	97.19	pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo]	[more]
XP_022933240.1	4.8e-88	96.63	pectinesterase inhibitor-like [Cucurbita moschata]	[more]
XP_022933241.1	6.3e-88	96.07	pectinesterase inhibitor-like [Cucurbita moschata] >XP_022933352.1 pectinesteras...	[more]
XP_022933238.1	1.4e-87	96.63	pectinesterase inhibitor-like [Cucurbita moschata]	[more]
XP_022933243.1	7.0e-87	94.94	pectinesterase inhibitor-like [Cucurbita moschata]	[more]

BLAST of CmoCh01G008890 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G48020.1	6.6e-19	36.36	pectin methylesterase inhibitor 1	[more]
AT3G17220.1	2.1e-17	34.52	pectin methylesterase inhibitor 2	[more]
AT3G36659.1	1.7e-06	29.58	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT2G47340.1	8.4e-06	29.07	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G17152.1	2.7e-04	27.21	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 29..171 e-value: 8.8E-32 score: 121.6
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 33..171 e-value: 1.9E-24 score: 86.7
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 10..175 e-value: 1.4E-33 score: 113.7
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 29..177 e-value: 1.0E-38 score: 134.7
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 32..176
None	No IPR available	PANTHER	PTHR36710:SF6	PECTINESTERASE INHIBITOR-LIKE	coord: 3..175
None	No IPR available	PANTHER	PTHR36710	PECTINESTERASE INHIBITOR-LIKE	coord: 3..175
IPR034086	Pectinesterase inhibitor, plant	CDD	cd15797	PMEI	coord: 31..175 e-value: 2.87272E-47 score: 149.495

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh01G008890.1	CmoCh01G008890.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity