CmoCh01G002550 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh01G002550
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	acyl-CoA--sterol O-acyltransferase 1-like
Location	Cmo_Chr01: 1156664 .. 1158018 (+)
RNA-Seq Expression	CmoCh01G002550
Synteny	CmoCh01G002550

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGAACAGGCCACCATTTTTGAAGTCTTTTTCGATCTCAAGCTTCTCGGATTCTCCATTAATGGCGGCTGAGATTCTGATCTTCCTTCAAACTCTGCTTCAGGTTTCATCGTCCCTCTGTTTCTGTTTCTCAATTAGAAACTCAATCCCCAATGGATTCCTCAAATTCCTCTTAATCCTCCCTTTCTTCCTCCTCTTCCTCTACATTCCTCTGCAATTTCAGACCCTACATCTTCAGGGCGCCATCGGCTTCTTCATCGGCTGGCTTGCCAGTTTCAAGCTCCTCCTCTTTGCATTCGGCAAAGGTCCTCTCTGCTCCGCCGTCGCTTCCTCCTCCCTCCGCCGCTTCCTCCTCGTCGGTTCTCTGCCGATCGACATCTACGATCACGACTCGGCTTCGACCCGACCCGATACCAATTCGGTTCTTCCTCTGAGCTTATCGTCAAAATTGTTCTTCCTAAGTCTCTCCATCGCAGCAATCTATTGCAAGAATTACTTCCATCCAAACGGAACCCTAATCGTTTTCTGCTTCGTAATTTACCTCCTCCTTGAAATTGTCCTCGGCGGAGCCGCCGCCGTTGCCAAGGCAACGCTCGGAGTCGAGTTATTGCCTTGTTTCGACGAGCCCTATTTATCTTCTTCGTTACAGGACTTTTGGGGTCGGCGATGGAATTTAATGGTCTCCAGAATCCTCCGCCTCTCCGTCTACGATCCATGCCGGAATTTCGCCGCGGCCATCGTCGGGAGGAAACCGGCGGCGATAATGGCGACGACGGCGACGTTCGCCGTCTCCGGTCTGATGCACGAACTGATATACTTCTACCTGGGGAGACTGGCGCCGACTTGGGAAGTAACTAGTTTCTTTCTGGTACATGGATTTTTCTTGGCGGTGGAGATGGCGGTGAAGAATGCAGTCGGTGAGAGATTCCGGCCGCCGCGAATTGTGACGGTGCCGCTGACGATCGGGTTCGTGATGGTGACGAGTTTTTGGCTGTTTTTCCCGCAGTTTTTCAGGTTTAAACCGGACGTTAGAATGTTTGAAGAGCACGCGGCTATGGGCGCGTTTGTTAAGAATGTGGCGGCCGAGTTGATCACATCCGTCAAACTCATCTCATTTCAAGTTAAATAATTGTTTTTACATTTTCAAGGAAATAAATAAATAAATAATTGGATTAAATTGTACTTGTTACAAAATGTGTAATATTTAATCTTTTATTTTTATAAATATTAAAATATATATATATATATATATATATATATATATATATATATATATATAAACACTATTTAAAAAAAATTAATCTAATTTTATCTTAAGCTTTTTTAATTAATTTATTTAAATCATATGTAGGCATGA

mRNA sequence

AGAACAGGCCACCATTTTTGAAGTCTTTTTCGATCTCAAGCTTCTCGGATTCTCCATTAATGGCGGCTGAGATTCTGATCTTCCTTCAAACTCTGCTTCAGGTTTCATCGTCCCTCTGTTTCTGTTTCTCAATTAGAAACTCAATCCCCAATGGATTCCTCAAATTCCTCTTAATCCTCCCTTTCTTCCTCCTCTTCCTCTACATTCCTCTGCAATTTCAGACCCTACATCTTCAGGGCGCCATCGGCTTCTTCATCGGCTGGCTTGCCAGTTTCAAGCTCCTCCTCTTTGCATTCGGCAAAGGTCCTCTCTGCTCCGCCGTCGCTTCCTCCTCCCTCCGCCGCTTCCTCCTCGTCGGTTCTCTGCCGATCGACATCTACGATCACGACTCGGCTTCGACCCGACCCGATACCAATTCGGTTCTTCCTCTGAGCTTATCGTCAAAATTGTTCTTCCTAAGTCTCTCCATCGCAGCAATCTATTGCAAGAATTACTTCCATCCAAACGGAACCCTAATCGTTTTCTGCTTCGTAATTTACCTCCTCCTTGAAATTGTCCTCGGCGGAGCCGCCGCCGTTGCCAAGGCAACGCTCGGAGTCGAGTTATTGCCTTGTTTCGACGAGCCCTATTTATCTTCTTCGTTACAGGACTTTTGGGGTCGGCGATGGAATTTAATGGTCTCCAGAATCCTCCGCCTCTCCGTCTACGATCCATGCCGGAATTTCGCCGCGGCCATCGTCGGGAGGAAACCGGCGGCGATAATGGCGACGACGGCGACGTTCGCCGTCTCCGGTCTGATGCACGAACTGATATACTTCTACCTGGGGAGACTGGCGCCGACTTGGGAAGTAACTAGTTTCTTTCTGGTACATGGATTTTTCTTGGCGGTGGAGATGGCGGTGAAGAATGCAGTCGGTGAGAGATTCCGGCCGCCGCGAATTGTGACGGTGCCGCTGACGATCGGGTTCGTGATGGTGACGAGTTTTTGGCTGTTTTTCCCGCAGTTTTTCAGGTTTAAACCGGACGTTAGAATGTTTGAAGAGCACGCGGCTATGGGCGCGTTTGTTAAGAATGTGGCGGCCGAGTTGATCACATCCGTCAAACTCATCTCATTTCAAGCATGA

Coding sequence (CDS)

ATGGCGGCTGAGATTCTGATCTTCCTTCAAACTCTGCTTCAGGTTTCATCGTCCCTCTGTTTCTGTTTCTCAATTAGAAACTCAATCCCCAATGGATTCCTCAAATTCCTCTTAATCCTCCCTTTCTTCCTCCTCTTCCTCTACATTCCTCTGCAATTTCAGACCCTACATCTTCAGGGCGCCATCGGCTTCTTCATCGGCTGGCTTGCCAGTTTCAAGCTCCTCCTCTTTGCATTCGGCAAAGGTCCTCTCTGCTCCGCCGTCGCTTCCTCCTCCCTCCGCCGCTTCCTCCTCGTCGGTTCTCTGCCGATCGACATCTACGATCACGACTCGGCTTCGACCCGACCCGATACCAATTCGGTTCTTCCTCTGAGCTTATCGTCAAAATTGTTCTTCCTAAGTCTCTCCATCGCAGCAATCTATTGCAAGAATTACTTCCATCCAAACGGAACCCTAATCGTTTTCTGCTTCGTAATTTACCTCCTCCTTGAAATTGTCCTCGGCGGAGCCGCCGCCGTTGCCAAGGCAACGCTCGGAGTCGAGTTATTGCCTTGTTTCGACGAGCCCTATTTATCTTCTTCGTTACAGGACTTTTGGGGTCGGCGATGGAATTTAATGGTCTCCAGAATCCTCCGCCTCTCCGTCTACGATCCATGCCGGAATTTCGCCGCGGCCATCGTCGGGAGGAAACCGGCGGCGATAATGGCGACGACGGCGACGTTCGCCGTCTCCGGTCTGATGCACGAACTGATATACTTCTACCTGGGGAGACTGGCGCCGACTTGGGAAGTAACTAGTTTCTTTCTGGTACATGGATTTTTCTTGGCGGTGGAGATGGCGGTGAAGAATGCAGTCGGTGAGAGATTCCGGCCGCCGCGAATTGTGACGGTGCCGCTGACGATCGGGTTCGTGATGGTGACGAGTTTTTGGCTGTTTTTCCCGCAGTTTTTCAGGTTTAAACCGGACGTTAGAATGTTTGAAGAGCACGCGGCTATGGGCGCGTTTGTTAAGAATGTGGCGGCCGAGTTGATCACATCCGTCAAACTCATCTCATTTCAAGCATGA

Protein sequence

MAAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQGAIGFFIGWLASFKLLLFAFGKGPLCSAVASSSLRRFLLVGSLPIDIYDHDSASTRPDTNSVLPLSLSSKLFFLSLSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGVELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTATFAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRIVTVPLTIGFVMVTSFWLFFPQFFRFKPDVRMFEEHAAMGAFVKNVAAELITSVKLISFQA

Homology

[Close]

BLAST of CmoCh01G002550 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 1.3e-63
Identity = 143/338 (42.31%), Postives = 200/338 (59.17%), Query Frame = 0


Query: 8   FLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQGAIGFFIG 67
           F++    V  SLC+ F I   +P G  + +L  P FL+F  +P    +LHL G   FFI 
Sbjct: 4   FIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFIA 63

Query: 68  WLASFKLLLFAFGKGPLCSAVASSSLRRFLLVGSLPIDIY--DHDSASTRPDTNSVLPLS 127
           WLA+FKLLLFA G+GPL S     SL  FL V  LPI I      + +   + ++  PL 
Sbjct: 64  WLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGSTEGPLI 123

Query: 128 LSSKLFFLSLSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGVELLPC 187
            + K  F+ L I A            L ++   IY  LEI+L   AA  +A   +EL P 
Sbjct: 124 YTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLELEPQ 183

Query: 188 FDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTATFAVSG 247
           F++PYL++SLQDFWGRRWNLMV+ ILR +VY+P      +++G   + I+A   TF VSG
Sbjct: 184 FNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQL-FSVLGPNYSQILAAFGTFVVSG 243

Query: 248 LMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRIVTVPLTIGFVM 307
           +MHELI+FY+GRL P W++  FFL++GF   VE+A+K  +  R+R P+ ++  LT+ FVM
Sbjct: 244 IMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLTFVM 303

Query: 308 VTSFWLFFPQFFRFKPDVRMFEEHAAMGAFVKNVAAEL 344
           VT+ WLF P+F R     +  +E+AA+GAF   V  +L
Sbjct: 304 VTALWLFLPEFNRCNIVEKALDEYAAIGAFAVEVRRKL 340

[Close]

BLAST of CmoCh01G002550 vs. ExPASy Swiss-Prot
Match: Q9XGY6 (Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 9.6e-59
Identity = 141/337 (41.84%), Postives = 196/337 (58.16%), Query Frame = 0


Query: 4   EILIFLQTLLQVSSSLCFCFSIRNSIPN-GFLKFLLILPFFLLFLYIPLQFQTLHLQGAI 63
           E+  F +  +   ++ C+C  +    P+ G L+ LL+LP  LLF+++PL+  + HL G  
Sbjct: 6   ELKTFSEVWISAIAAACYCRFVPAVAPHGGALRLLLLLPVVLLFIFLPLRLSSFHLGGPT 65

Query: 64  GFFIGWLASFKLLLFAFGKGPLCSAVASSSLRRFLLVGSLPIDIYDHDSASTRPDTNSV- 123
             ++ WLA+FKLLLFAF  GPL +   S SL  F+    LPI   D  S     +  ++ 
Sbjct: 66  ALYLVWLANFKLLLFAFHLGPLSN--PSLSLLHFISTTLLPIKFRDDPSNDHEKNKRTLS 125

Query: 124 -----LPLSLSSKLFFLSLSIAAIYCKNYFHPNGTL-IVFCFVIYLLLEIVLGGAAAVAK 183
                + L ++  +FF    I  IY      P+  + +++CF  YL  EI L  +A +A+
Sbjct: 126 FEWRKVVLFVAKLVFF--AGILKIYEFRKDLPHFVISVLYCFHFYLGTEITLAASAVIAR 185

Query: 184 ATLGVELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIM 243
           ATLG++L P F+EPYL++SLQDFWGRRWNLMVS IL L+ Y P R   +  V  +     
Sbjct: 186 ATLGLDLYPQFNEPYLATSLQDFWGRRWNLMVSDILGLTTYQPVRRVLSRWVRLRWEVAG 245

Query: 244 ATTATFAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRIV 303
           A    F VSGLMHE+ +FYL R  P+WEVT FF++HG   AVEM VK AV  + R  R V
Sbjct: 246 AMLVAFTVSGLMHEVFFFYLTRARPSWEVTGFFVLHGVCTAVEMVVKKAVSGKVRLRREV 305

Query: 304 TVPLTIGFVMVTSFWLFFPQFFRFKPDVRMFEEHAAM 333
           +  LT+GFVMVT  WLF PQ  R   D++  +E+  M
Sbjct: 306 SGALTVGFVMVTGGWLFLPQLVRHGVDLKTIDEYPVM 338

[Close]

BLAST of CmoCh01G002550 vs. ExPASy Swiss-Prot
Match: Q9FJ75 (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=AT4 PE=2 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 1.6e-50
Identity = 125/339 (36.87%), Postives = 186/339 (54.87%), Query Frame = 0


Query: 1   MAAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG 60
           M  E++  ++  +    S+ +C+     I +G  + L +LP  +LFL +PL   ++H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  AIGFFIGWLASFKLLLFAFGKGPLCSAVASSSLRRFLLVGSLPIDIYDHDSASTRPDTNS 120
           +  FF+ WLA+FKL+LF+F +GPL      S+L RF+     PI +  +         N 
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLFP--VPSNLSRFVCFTCFPIKLQQNPKPQ-----NQ 120

Query: 121 VLPLSLSSKLFFLSLSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGV 180
           +     + KL F  + +     K +  P   L+++   IYL  EI+L     +   +L  
Sbjct: 121 MPKWGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISLWC 180

Query: 181 ELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTAT 240
           +L P F+EPYLS+SLQDFWGRRWNLMV  ILR +VY P R  A   +    A  +   A+
Sbjct: 181 DLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVFAS 240

Query: 241 FAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNA-VGERFRPPRIVTVPL 300
           F VSG++HELI+FY  R +PT EVT FF++HG   A E A K   +  R++  ++V+  L
Sbjct: 241 FLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQMVSRLL 300

Query: 301 TIGFVMVTSFWLFFPQFFRFKPDVRMFEEHAAMGAFVKN 339
           T+GFV++T  WLFFP   R     R+ +E      FVK+
Sbjct: 301 TVGFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVKH 332

[Close]

BLAST of CmoCh01G002550 vs. ExPASy Swiss-Prot
Match: Q9FJ72 (Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=AT1 PE=2 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 4.8e-50
Identity = 125/307 (40.72%), Postives = 171/307 (55.70%), Query Frame = 0


Query: 18  SLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQGAIGFFIGWLASFKLLLF 77
           SL +C+ I + +  G L+ L ILP  +LFL +PL    +H       F+ WLA+FKLLLF
Sbjct: 18  SLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCAISVLFLSWLANFKLLLF 77

Query: 78  AFGKGPLCSAVASSSLRRFLLVGSLPIDIYDHDSASTRPDTNSVLPLSLSSKLFFLSLSI 137
           AF +GPL        L RF+    LPI I    S +  P+ +   P  +   +  + + +
Sbjct: 78  AFDEGPLFP--LPPKLSRFICFACLPIKIRQDPSPNAIPNLH---PKPMPKWVLAVKILV 137

Query: 138 AAIYCKNYFHPNG-----TLIVFCFVIYLLLEIVLGGAAAVAKATLGVELLPCFDEPYLS 197
             +    Y + +G      L ++C  IYL +E+VL    AV    LG  + P F+EPYL+
Sbjct: 138 LGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVVSTLLGCNIEPVFNEPYLA 197

Query: 198 SSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTATFAVSGLMHELIY 257
           +SLQDFW RRWNLMVS +LR +V+ P + F   I+    A      A+F VSGLMHEL+Y
Sbjct: 198 TSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMFAGVMASFFVSGLMHELLY 257

Query: 258 FYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPP-RIVTVPLTIGFVMVTSFWL 317
           FY+ R  PTWEVT FF++HG   A E+AVK    +  RPP R V+  + + FV VT  WL
Sbjct: 258 FYMIRKPPTWEVTCFFVLHGAATATEIAVKRT--QWLRPPHRAVSGLVVLTFVSVTGVWL 317

Query: 318 FFPQFFR 319
           F  Q  R
Sbjct: 318 FLAQVLR 317

[Close]

BLAST of CmoCh01G002550 vs. ExPASy Swiss-Prot
Match: Q9FJ73 (Probable long-chain-alcohol O-fatty-acyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=AT2 PE=3 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 6.3e-50
Identity = 131/333 (39.34%), Postives = 186/333 (55.86%), Query Frame = 0


Query: 1   MAAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG 60
           M  E+   ++  +    S+ +C+ I + I  G L+ L +LP F++FL +PL F ++H   
Sbjct: 1   MEEELRNLIKVWISALISISYCYYISSKISKGVLRLLSLLPIFIIFLLLPLFFSSVHFCV 60

Query: 61  AIGFFIGWLASFKLLLFAFGKGPLCSAVASSSLRRFLLVGSLPIDIYDHDSASTRPDTNS 120
             GFF  WLA+FKL LFAF + PL  +   S+L RF      PI I + + +S R     
Sbjct: 61  ISGFFFTWLANFKLFLFAFDQEPL--SPLPSNLTRFFCFACFPIKI-NKNPSSNRIHNKP 120

Query: 121 VLPLSLSSKLFFLSLSIAAIYCKNY---FHPNGTLIVFCFVIYLLLEIVLGGAAAVAKAT 180
           +    L+ KL   S  +  +Y  NY         L +F   +YL  E++L    A+    
Sbjct: 121 MSKWVLAFKLLIFSF-LLHVYRNNYDSGLSRFAFLALFTIHVYLEAELILVFVGALMSML 180

Query: 181 LGVELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMAT 240
           LG E+ P F++PYL++SLQ+FW RRWNLMV  +LR +V+ P + F A ++G   A     
Sbjct: 181 LGCEMEPVFNDPYLATSLQEFWSRRWNLMVPAVLRPAVHIPVQRFCAPLLGLHRAFYAGM 240

Query: 241 TATFAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRP-PRIVT 300
            ATF VSGLMHELIYFY+ R +PTWEVT FFL+HG    +E+A+K     R+ P PR   
Sbjct: 241 LATFIVSGLMHELIYFYVIRKSPTWEVTCFFLLHGVVTCLEIAMKRM---RWLPTPRRAV 300

Query: 301 VPLTIG-FVMVTSFWLFFPQFFRFKPDVRMFEE 329
             L I  F++VT+ WLF+PQ  R     R+  E
Sbjct: 301 SGLAITVFLLVTAGWLFYPQMLRNDVHKRVISE 326

[Close]

BLAST of CmoCh01G002550 vs. ExPASy TrEMBL
Match: A0A6J1GBB6 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111452615 PE=3 SV=1)

HSP 1 Score: 684.1 bits (1764), Expect = 3.1e-193
Identity = 353/353 (100.00%), Postives = 353/353 (100.00%), Query Frame = 0


Query: 1   MAAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG 60
           MAAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG
Sbjct: 1   MAAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG 60

Query: 61  AIGFFIGWLASFKLLLFAFGKGPLCSAVASSSLRRFLLVGSLPIDIYDHDSASTRPDTNS 120
           AIGFFIGWLASFKLLLFAFGKGPLCSAVASSSLRRFLLVGSLPIDIYDHDSASTRPDTNS
Sbjct: 61  AIGFFIGWLASFKLLLFAFGKGPLCSAVASSSLRRFLLVGSLPIDIYDHDSASTRPDTNS 120

Query: 121 VLPLSLSSKLFFLSLSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGV 180
           VLPLSLSSKLFFLSLSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGV
Sbjct: 121 VLPLSLSSKLFFLSLSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGV 180

Query: 181 ELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTAT 240
           ELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTAT
Sbjct: 181 ELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTAT 240

Query: 241 FAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRIVTVPLT 300
           FAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRIVTVPLT
Sbjct: 241 FAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRIVTVPLT 300

Query: 301 IGFVMVTSFWLFFPQFFRFKPDVRMFEEHAAMGAFVKNVAAELITSVKLISFQ 354
           IGFVMVTSFWLFFPQFFRFKPDVRMFEEHAAMGAFVKNVAAELITSVKLISFQ
Sbjct: 301 IGFVMVTSFWLFFPQFFRFKPDVRMFEEHAAMGAFVKNVAAELITSVKLISFQ 353

[Close]

BLAST of CmoCh01G002550 vs. ExPASy TrEMBL
Match: A0A6J1KCL2 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493090 PE=3 SV=1)

HSP 1 Score: 662.5 bits (1708), Expect = 9.6e-187
Identity = 342/353 (96.88%), Postives = 344/353 (97.45%), Query Frame = 0


Query: 1   MAAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG 60
           MAAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG
Sbjct: 1   MAAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG 60

Query: 61  AIGFFIGWLASFKLLLFAFGKGPLCSAVASSSLRRFLLVGSLPIDIYDHDSASTRPDTNS 120
           AIGFFIGWLASFKLLLFAFGKG LCSA ASSSL RFLLVGSLPIDIYDHD  STRPDT S
Sbjct: 61  AIGFFIGWLASFKLLLFAFGKGSLCSAAASSSLSRFLLVGSLPIDIYDHDPDSTRPDTTS 120

Query: 121 VLPLSLSSKLFFLSLSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGV 180
           VLPLSLSSKLFFL LSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGV
Sbjct: 121 VLPLSLSSKLFFLILSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGV 180

Query: 181 ELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTAT 240
           ELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMA TAT
Sbjct: 181 ELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMAMTAT 240

Query: 241 FAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRIVTVPLT 300
           FAVSGLMHELIYFY+GRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRIVTVPLT
Sbjct: 241 FAVSGLMHELIYFYMGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRIVTVPLT 300

Query: 301 IGFVMVTSFWLFFPQFFRFKPDVRMFEEHAAMGAFVKNVAAELITSVKLISFQ 354
           IGFVMVTSFWLFFPQFFRFKPDVRMFEEHAA+GAFVKNVA ELITSVKLISFQ
Sbjct: 301 IGFVMVTSFWLFFPQFFRFKPDVRMFEEHAALGAFVKNVAGELITSVKLISFQ 353

[Close]

BLAST of CmoCh01G002550 vs. ExPASy TrEMBL
Match: A0A6J1IX47 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111481451 PE=3 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 2.0e-136
Identity = 255/353 (72.24%), Postives = 290/353 (82.15%), Query Frame = 0


Query: 1   MAAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG 60
           MAAEI  F+QTL  +SSSLC CFSIRNSIPNGFLKF+ +LPFF LFLYIPLQFQT+HL+G
Sbjct: 1   MAAEIHAFIQTLFLISSSLCLCFSIRNSIPNGFLKFIFVLPFFFLFLYIPLQFQTIHLRG 60

Query: 61  AIGFFIGWLASFKLLLFAFGKGPLCSAVASSSLRRFLLVGSLPIDIYDHDSASTRPDTNS 120
            +GFFIGWL SFKLLLFAFGKGPLCSA  SSSLRRFL V SLPIDI +H     R D + 
Sbjct: 61  TLGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVASLPIDIPEHKPPPNRSDPDP 120

Query: 121 VLPLSLSSKLFFLSLSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGV 180
           +LPLS ++KL FL L+ AAIY KN F+PN  LI FC +IYLLLEI++GGA AVA+A LGV
Sbjct: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATAVAEAVLGV 180

Query: 181 ELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTAT 240
           ELLP FDEPYLS SLQDFWGRRWNL+ SRILR +V+DPCR  A  +VG+KPA I+A  AT
Sbjct: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAVIIAVMAT 240

Query: 241 FAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRIVTVPLT 300
           FAVSG+MHELIYFY+GRLAPTWEV+ FFL+HG  LA EMAVK+AVG RFR PR+V+  LT
Sbjct: 241 FAVSGVMHELIYFYMGRLAPTWEVSWFFLIHGVCLAAEMAVKSAVGRRFRAPRVVSGVLT 300

Query: 301 IGFVMVTSFWLFFPQFFRFKPDVRMFEEHAAMGAFVKNVAAELITSVKLISFQ 354
           +GFVM TSFWLFFPQ  RFK DVRM EE+AA+GAFVKNV AELI  +  ISFQ
Sbjct: 301 MGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKNVTAELIGPL-FISFQ 352

[Close]

BLAST of CmoCh01G002550 vs. ExPASy TrEMBL
Match: A0A6J1F144 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111441426 PE=3 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 1.0e-135
Identity = 254/353 (71.95%), Postives = 290/353 (82.15%), Query Frame = 0


Query: 1   MAAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG 60
           MAAEI  F+QTL  ++SSLC CFSIRNSIPNGFLKFL +LPFF LFLYIPLQFQT+HL+G
Sbjct: 1   MAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRG 60

Query: 61  AIGFFIGWLASFKLLLFAFGKGPLCSAVASSSLRRFLLVGSLPIDIYDHDSASTRPDTNS 120
            +GFFIGWL SFKLLLFAFGKGPLC A  SSSLRRFL V  LPIDI +H S   R D + 
Sbjct: 61  TLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDP 120

Query: 121 VLPLSLSSKLFFLSLSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGV 180
           +LPLS ++KL FL L+ AAIY KN F+PN  LI FC +IYLLLEI++GGA A+A+A LGV
Sbjct: 121 LLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGV 180

Query: 181 ELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTAT 240
           ELLP FDEPYLS SLQDFWGRRWNL+ SRILR +V+DPCR  A  +VG+KPAAI+A  AT
Sbjct: 181 ELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMAT 240

Query: 241 FAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRIVTVPLT 300
           FAVSG+MHELIYFY+GRL PTWEV+ FFL+HG  LA EMAVK+AVG RFRPPR+V+  LT
Sbjct: 241 FAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGVLT 300

Query: 301 IGFVMVTSFWLFFPQFFRFKPDVRMFEEHAAMGAFVKNVAAELITSVKLISFQ 354
           IGFVM TSFWLFFPQ  RFK DVRM EE+AA+GAFVK+V AELI  +  ISFQ
Sbjct: 301 IGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKSVTAELIGPL-FISFQ 352

[Close]

BLAST of CmoCh01G002550 vs. ExPASy TrEMBL
Match: A0A0A0LES4 (MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G684670 PE=3 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 9.5e-134
Identity = 255/348 (73.28%), Postives = 284/348 (81.61%), Query Frame = 0


Query: 1   MAAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG 60
           MAAEI IFLQTLL VSSSL FCFSIRNSIPNGFL+FLLILPFF LFLYIPLQFQT+HLQG
Sbjct: 1   MAAEIRIFLQTLLLVSSSLSFCFSIRNSIPNGFLRFLLILPFFSLFLYIPLQFQTIHLQG 60

Query: 61  AIGFFIGWLASFKLLLFAFGKGPLCSAVASSSLRRFLLVGSLPIDIYDHDSASTRPDTNS 120
           +IGFFIGWLASFKLLLFAFGKGPLCSA ASSSLRRFL +GSLPI+I      + R   N 
Sbjct: 61  SIGFFIGWLASFKLLLFAFGKGPLCSAAASSSLRRFLAIGSLPIEI---PQDTNRSHPNP 120

Query: 121 VLPLSLSSKLFFLSLSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGV 180
           +LP +   KL  L L++ AIY KNY HP   LIVFCFVIY LLEI++GGAAA  KATLGV
Sbjct: 121 ILPPTYFIKLLLLILTLVAIYFKNYLHPTLYLIVFCFVIYFLLEILIGGAAAFVKATLGV 180

Query: 181 ELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTAT 240
           ELLP FDEPYLS SLQDFWG+RWNL+ SRILRL+VYDPCR     IVG+K +A++A TAT
Sbjct: 181 ELLPYFDEPYLSVSLQDFWGKRWNLLTSRILRLAVYDPCRKLTVGIVGKKASAMVALTAT 240

Query: 241 FAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRIVTVPLT 300
           FAVSGLMHELIYFY+GRLAPTWEVT FFL+HG  L+ EMA+K+AV  R + PRI++   T
Sbjct: 241 FAVSGLMHELIYFYMGRLAPTWEVTCFFLLHGVSLSAEMAMKSAVRGRLKVPRIISTGFT 300

Query: 301 IGFVMVTSFWLFFPQFFRFKPDVRMFEEHAAMGAFVKNVAAELITSVK 349
             FVM TS WLFFPQFFR KPDVRM EEHAA+GAFVKNV  +LIT  K
Sbjct: 301 WWFVMTTSSWLFFPQFFRLKPDVRMMEEHAALGAFVKNVTVQLITPFK 345

[Close]

BLAST of CmoCh01G002550 vs. NCBI nr
Match: XP_022949191.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata])

HSP 1 Score: 684.1 bits (1764), Expect = 6.4e-193
Identity = 353/353 (100.00%), Postives = 353/353 (100.00%), Query Frame = 0


Query: 1   MAAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG 60
           MAAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG
Sbjct: 1   MAAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG 60

Query: 61  AIGFFIGWLASFKLLLFAFGKGPLCSAVASSSLRRFLLVGSLPIDIYDHDSASTRPDTNS 120
           AIGFFIGWLASFKLLLFAFGKGPLCSAVASSSLRRFLLVGSLPIDIYDHDSASTRPDTNS
Sbjct: 61  AIGFFIGWLASFKLLLFAFGKGPLCSAVASSSLRRFLLVGSLPIDIYDHDSASTRPDTNS 120

Query: 121 VLPLSLSSKLFFLSLSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGV 180
           VLPLSLSSKLFFLSLSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGV
Sbjct: 121 VLPLSLSSKLFFLSLSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGV 180

Query: 181 ELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTAT 240
           ELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTAT
Sbjct: 181 ELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTAT 240

Query: 241 FAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRIVTVPLT 300
           FAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRIVTVPLT
Sbjct: 241 FAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRIVTVPLT 300

Query: 301 IGFVMVTSFWLFFPQFFRFKPDVRMFEEHAAMGAFVKNVAAELITSVKLISFQ 354
           IGFVMVTSFWLFFPQFFRFKPDVRMFEEHAAMGAFVKNVAAELITSVKLISFQ
Sbjct: 301 IGFVMVTSFWLFFPQFFRFKPDVRMFEEHAAMGAFVKNVAAELITSVKLISFQ 353

[Close]

BLAST of CmoCh01G002550 vs. NCBI nr
Match: KAG7036600.1 (Acyl-CoA--sterol O-acyltransferase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 680.6 bits (1755), Expect = 7.1e-192
Identity = 351/353 (99.43%), Postives = 351/353 (99.43%), Query Frame = 0


Query: 1   MAAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG 60
           MAAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG
Sbjct: 552 MAAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG 611

Query: 61  AIGFFIGWLASFKLLLFAFGKGPLCSAVASSSLRRFLLVGSLPIDIYDHDSASTRPDTNS 120
           AIGFFIGWLASFKLLLFAFGKGPLCSAVASSSLRRFLLVGSLPIDIYDHD ASTRPDTNS
Sbjct: 612 AIGFFIGWLASFKLLLFAFGKGPLCSAVASSSLRRFLLVGSLPIDIYDHDPASTRPDTNS 671

Query: 121 VLPLSLSSKLFFLSLSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGV 180
           VLPLSLSSKLFFLSLSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGG AAVAKATLGV
Sbjct: 672 VLPLSLSSKLFFLSLSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGVAAVAKATLGV 731

Query: 181 ELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTAT 240
           ELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTAT
Sbjct: 732 ELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTAT 791

Query: 241 FAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRIVTVPLT 300
           FAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRIVTVPLT
Sbjct: 792 FAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRIVTVPLT 851

Query: 301 IGFVMVTSFWLFFPQFFRFKPDVRMFEEHAAMGAFVKNVAAELITSVKLISFQ 354
           IGFVMVTSFWLFFPQFFRFKPDVRMFEEHAAMGAFVKNVAAELITSVKLISFQ
Sbjct: 852 IGFVMVTSFWLFFPQFFRFKPDVRMFEEHAAMGAFVKNVAAELITSVKLISFQ 904

[Close]

BLAST of CmoCh01G002550 vs. NCBI nr
Match: KAG6606897.1 (Acyl-CoA--sterol O-acyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 680.6 bits (1755), Expect = 7.1e-192
Identity = 351/353 (99.43%), Postives = 351/353 (99.43%), Query Frame = 0


Query: 1   MAAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG 60
           MAAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG
Sbjct: 1   MAAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG 60

Query: 61  AIGFFIGWLASFKLLLFAFGKGPLCSAVASSSLRRFLLVGSLPIDIYDHDSASTRPDTNS 120
           AIGFFIGWLASFKLLLFAFGKGPLCSAVASSSLRRFLLVGSLPIDIYDHD ASTRPDTNS
Sbjct: 61  AIGFFIGWLASFKLLLFAFGKGPLCSAVASSSLRRFLLVGSLPIDIYDHDPASTRPDTNS 120

Query: 121 VLPLSLSSKLFFLSLSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGV 180
           VLPLSLSSKLFFLSLSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGG AAVAKATLGV
Sbjct: 121 VLPLSLSSKLFFLSLSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGVAAVAKATLGV 180

Query: 181 ELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTAT 240
           ELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTAT
Sbjct: 181 ELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTAT 240

Query: 241 FAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRIVTVPLT 300
           FAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRIVTVPLT
Sbjct: 241 FAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRIVTVPLT 300

Query: 301 IGFVMVTSFWLFFPQFFRFKPDVRMFEEHAAMGAFVKNVAAELITSVKLISFQ 354
           IGFVMVTSFWLFFPQFFRFKPDVRMFEEHAAMGAFVKNVAAELITSVKLISFQ
Sbjct: 301 IGFVMVTSFWLFFPQFFRFKPDVRMFEEHAAMGAFVKNVAAELITSVKLISFQ 353

[Close]

BLAST of CmoCh01G002550 vs. NCBI nr
Match: XP_023523548.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 676.8 bits (1745), Expect = 1.0e-190
Identity = 348/353 (98.58%), Postives = 350/353 (99.15%), Query Frame = 0


Query: 1   MAAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG 60
           MAAEILIFLQTLLQVSSSLCFC+SI+NSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG
Sbjct: 1   MAAEILIFLQTLLQVSSSLCFCYSIKNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG 60

Query: 61  AIGFFIGWLASFKLLLFAFGKGPLCSAVASSSLRRFLLVGSLPIDIYDHDSASTRPDTNS 120
           AIGFFIGWLASFKLLLFAFGKGPLCSA ASSSLRRFLLVGSLPIDIYDHD ASTRPDTNS
Sbjct: 61  AIGFFIGWLASFKLLLFAFGKGPLCSAAASSSLRRFLLVGSLPIDIYDHDPASTRPDTNS 120

Query: 121 VLPLSLSSKLFFLSLSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGV 180
           VLPLSLSSKLFFLSLSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGV
Sbjct: 121 VLPLSLSSKLFFLSLSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGV 180

Query: 181 ELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTAT 240
           ELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTAT
Sbjct: 181 ELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTAT 240

Query: 241 FAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRIVTVPLT 300
           FAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPR VTVPLT
Sbjct: 241 FAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRFVTVPLT 300

Query: 301 IGFVMVTSFWLFFPQFFRFKPDVRMFEEHAAMGAFVKNVAAELITSVKLISFQ 354
           IGFVMVTSFWLFFPQFFRFKPDVRMFEEHAAMGAFVKNVAAELITSVKLISFQ
Sbjct: 301 IGFVMVTSFWLFFPQFFRFKPDVRMFEEHAAMGAFVKNVAAELITSVKLISFQ 353

[Close]

BLAST of CmoCh01G002550 vs. NCBI nr
Match: XP_022998465.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 662.5 bits (1708), Expect = 2.0e-186
Identity = 342/353 (96.88%), Postives = 344/353 (97.45%), Query Frame = 0


Query: 1   MAAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG 60
           MAAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG
Sbjct: 1   MAAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG 60

Query: 61  AIGFFIGWLASFKLLLFAFGKGPLCSAVASSSLRRFLLVGSLPIDIYDHDSASTRPDTNS 120
           AIGFFIGWLASFKLLLFAFGKG LCSA ASSSL RFLLVGSLPIDIYDHD  STRPDT S
Sbjct: 61  AIGFFIGWLASFKLLLFAFGKGSLCSAAASSSLSRFLLVGSLPIDIYDHDPDSTRPDTTS 120

Query: 121 VLPLSLSSKLFFLSLSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGV 180
           VLPLSLSSKLFFL LSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGV
Sbjct: 121 VLPLSLSSKLFFLILSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGV 180

Query: 181 ELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTAT 240
           ELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMA TAT
Sbjct: 181 ELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMAMTAT 240

Query: 241 FAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRIVTVPLT 300
           FAVSGLMHELIYFY+GRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRIVTVPLT
Sbjct: 241 FAVSGLMHELIYFYMGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRIVTVPLT 300

Query: 301 IGFVMVTSFWLFFPQFFRFKPDVRMFEEHAAMGAFVKNVAAELITSVKLISFQ 354
           IGFVMVTSFWLFFPQFFRFKPDVRMFEEHAA+GAFVKNVA ELITSVKLISFQ
Sbjct: 301 IGFVMVTSFWLFFPQFFRFKPDVRMFEEHAALGAFVKNVAGELITSVKLISFQ 353

[Close]

BLAST of CmoCh01G002550 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 245.0 bits (624), Expect = 9.2e-65
Identity = 143/338 (42.31%), Postives = 200/338 (59.17%), Query Frame = 0


Query: 8   FLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQGAIGFFIG 67
           F++    V  SLC+ F I   +P G  + +L  P FL+F  +P    +LHL G   FFI 
Sbjct: 4   FIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFIA 63

Query: 68  WLASFKLLLFAFGKGPLCSAVASSSLRRFLLVGSLPIDIY--DHDSASTRPDTNSVLPLS 127
           WLA+FKLLLFA G+GPL S     SL  FL V  LPI I      + +   + ++  PL 
Sbjct: 64  WLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGSTEGPLI 123

Query: 128 LSSKLFFLSLSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGVELLPC 187
            + K  F+ L I A            L ++   IY  LEI+L   AA  +A   +EL P 
Sbjct: 124 YTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLELEPQ 183

Query: 188 FDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTATFAVSG 247
           F++PYL++SLQDFWGRRWNLMV+ ILR +VY+P      +++G   + I+A   TF VSG
Sbjct: 184 FNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQL-FSVLGPNYSQILAAFGTFVVSG 243

Query: 248 LMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRIVTVPLTIGFVM 307
           +MHELI+FY+GRL P W++  FFL++GF   VE+A+K  +  R+R P+ ++  LT+ FVM
Sbjct: 244 IMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLTFVM 303

Query: 308 VTSFWLFFPQFFRFKPDVRMFEEHAAMGAFVKNVAAEL 344
           VT+ WLF P+F R     +  +E+AA+GAF   V  +L
Sbjct: 304 VTALWLFLPEFNRCNIVEKALDEYAAIGAFAVEVRRKL 340

[Close]

BLAST of CmoCh01G002550 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 201.4 bits (511), Expect = 1.2e-51
Identity = 125/339 (36.87%), Postives = 186/339 (54.87%), Query Frame = 0


Query: 1   MAAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG 60
           M  E++  ++  +    S+ +C+     I +G  + L +LP  +LFL +PL   ++H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  AIGFFIGWLASFKLLLFAFGKGPLCSAVASSSLRRFLLVGSLPIDIYDHDSASTRPDTNS 120
           +  FF+ WLA+FKL+LF+F +GPL      S+L RF+     PI +  +         N 
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLFP--VPSNLSRFVCFTCFPIKLQQNPKPQ-----NQ 120

Query: 121 VLPLSLSSKLFFLSLSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGV 180
           +     + KL F  + +     K +  P   L+++   IYL  EI+L     +   +L  
Sbjct: 121 MPKWGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISLWC 180

Query: 181 ELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTAT 240
           +L P F+EPYLS+SLQDFWGRRWNLMV  ILR +VY P R  A   +    A  +   A+
Sbjct: 181 DLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVFAS 240

Query: 241 FAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNA-VGERFRPPRIVTVPL 300
           F VSG++HELI+FY  R +PT EVT FF++HG   A E A K   +  R++  ++V+  L
Sbjct: 241 FLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQMVSRLL 300

Query: 301 TIGFVMVTSFWLFFPQFFRFKPDVRMFEEHAAMGAFVKN 339
           T+GFV++T  WLFFP   R     R+ +E      FVK+
Sbjct: 301 TVGFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVKH 332

[Close]

BLAST of CmoCh01G002550 vs. TAIR 10
Match: AT5G55380.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 199.9 bits (507), Expect = 3.4e-51
Identity = 125/307 (40.72%), Postives = 171/307 (55.70%), Query Frame = 0


Query: 18  SLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQGAIGFFIGWLASFKLLLF 77
           SL +C+ I + +  G L+ L ILP  +LFL +PL    +H       F+ WLA+FKLLLF
Sbjct: 18  SLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCAISVLFLSWLANFKLLLF 77

Query: 78  AFGKGPLCSAVASSSLRRFLLVGSLPIDIYDHDSASTRPDTNSVLPLSLSSKLFFLSLSI 137
           AF +GPL        L RF+    LPI I    S +  P+ +   P  +   +  + + +
Sbjct: 78  AFDEGPLFP--LPPKLSRFICFACLPIKIRQDPSPNAIPNLH---PKPMPKWVLAVKILV 137

Query: 138 AAIYCKNYFHPNG-----TLIVFCFVIYLLLEIVLGGAAAVAKATLGVELLPCFDEPYLS 197
             +    Y + +G      L ++C  IYL +E+VL    AV    LG  + P F+EPYL+
Sbjct: 138 LGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVVSTLLGCNIEPVFNEPYLA 197

Query: 198 SSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTATFAVSGLMHELIY 257
           +SLQDFW RRWNLMVS +LR +V+ P + F   I+    A      A+F VSGLMHEL+Y
Sbjct: 198 TSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMFAGVMASFFVSGLMHELLY 257

Query: 258 FYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPP-RIVTVPLTIGFVMVTSFWL 317
           FY+ R  PTWEVT FF++HG   A E+AVK    +  RPP R V+  + + FV VT  WL
Sbjct: 258 FYMIRKPPTWEVTCFFVLHGAATATEIAVKRT--QWLRPPHRAVSGLVVLTFVSVTGVWL 317

Query: 318 FFPQFFR 319
           F  Q  R
Sbjct: 318 FLAQVLR 317

[Close]

BLAST of CmoCh01G002550 vs. TAIR 10
Match: AT5G55370.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 199.5 bits (506), Expect = 4.5e-51
Identity = 131/333 (39.34%), Postives = 186/333 (55.86%), Query Frame = 0


Query: 1   MAAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG 60
           M  E+   ++  +    S+ +C+ I + I  G L+ L +LP F++FL +PL F ++H   
Sbjct: 1   MEEELRNLIKVWISALISISYCYYISSKISKGVLRLLSLLPIFIIFLLLPLFFSSVHFCV 60

Query: 61  AIGFFIGWLASFKLLLFAFGKGPLCSAVASSSLRRFLLVGSLPIDIYDHDSASTRPDTNS 120
             GFF  WLA+FKL LFAF + PL  +   S+L RF      PI I + + +S R     
Sbjct: 61  ISGFFFTWLANFKLFLFAFDQEPL--SPLPSNLTRFFCFACFPIKI-NKNPSSNRIHNKP 120

Query: 121 VLPLSLSSKLFFLSLSIAAIYCKNY---FHPNGTLIVFCFVIYLLLEIVLGGAAAVAKAT 180
           +    L+ KL   S  +  +Y  NY         L +F   +YL  E++L    A+    
Sbjct: 121 MSKWVLAFKLLIFSF-LLHVYRNNYDSGLSRFAFLALFTIHVYLEAELILVFVGALMSML 180

Query: 181 LGVELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMAT 240
           LG E+ P F++PYL++SLQ+FW RRWNLMV  +LR +V+ P + F A ++G   A     
Sbjct: 181 LGCEMEPVFNDPYLATSLQEFWSRRWNLMVPAVLRPAVHIPVQRFCAPLLGLHRAFYAGM 240

Query: 241 TATFAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRP-PRIVT 300
            ATF VSGLMHELIYFY+ R +PTWEVT FFL+HG    +E+A+K     R+ P PR   
Sbjct: 241 LATFIVSGLMHELIYFYVIRKSPTWEVTCFFLLHGVVTCLEIAMKRM---RWLPTPRRAV 300

Query: 301 VPLTIG-FVMVTSFWLFFPQFFRFKPDVRMFEE 329
             L I  F++VT+ WLF+PQ  R     R+  E
Sbjct: 301 SGLAITVFLLVTAGWLFYPQMLRNDVHKRVISE 326

[Close]

BLAST of CmoCh01G002550 vs. TAIR 10
Match: AT5G55360.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 193.0 bits (489), Expect = 4.2e-49
Identity = 125/317 (39.43%), Postives = 175/317 (55.21%), Query Frame = 0


Query: 1   MAAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQG 60
           M  E+  F++  +    S+ +C+ +   I  G  + L +LP   LFL  PL F  +H  G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  AIGFFIGWLASFKLLLFAFGKGPLCSAVASSSLRRFLLVGSLPIDIYDHDSASTRPDTNS 120
            + FF+ WLA+FKL+LF+F +GPL  +    +L RF+ +   PI    + +       N 
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPL--SPLPRTLSRFICITCFPIKPQQNPNIQ-----NY 120

Query: 121 VLPLSLSSKLFFLSLSIAAIY-CKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLG 180
            +P+ L +    + + +  +Y  K Y  P   L+     I+L LEIV     A+   TLG
Sbjct: 121 KIPIWLFAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKALVFITLG 180

Query: 181 VELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTA 240
            +L P  +EPYL++SLQDFWGRRWNLMV  ILR +VY P R  A   V    A  +   A
Sbjct: 181 CDLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQAMFLGVFA 240

Query: 241 TFAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGER--FRPPRIVTV 300
            F VSG +HE+++FYL R  PT EVT FFL+HG     E+AVK +   R  +R    V+ 
Sbjct: 241 AFLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWRVSPTVSR 300

Query: 301 PLTIGFVMVTSFWLFFP 315
            LT+GFV+VTS W FFP
Sbjct: 301 LLTVGFVVVTSGWFFFP 310

The following BLAST results are available for this feature:

BLAST of CmoCh01G002550 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SV07	1.3e-63	42.31	Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE...	[more]
Q9XGY6	9.6e-59	41.84	Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 ...	[more]
Q9FJ75	1.6e-50	36.87	Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX...	[more]
Q9FJ72	4.8e-50	40.72	Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX...	[more]
Q9FJ73	6.3e-50	39.34	Probable long-chain-alcohol O-fatty-acyltransferase 2 OS=Arabidopsis thaliana OX...	[more]

BLAST of CmoCh01G002550 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GBB6	3.1e-193	100.00	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1KCL2	9.6e-187	96.88	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1IX47	2.0e-136	72.24	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1F144	1.0e-135	71.95	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A0A0LES4	9.5e-134	73.28	MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G684670 PE=...	[more]

BLAST of CmoCh01G002550 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022949191.1	6.4e-193	100.00	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata]	[more]
KAG7036600.1	7.1e-192	99.43	Acyl-CoA--sterol O-acyltransferase 1, partial [Cucurbita argyrosperma subsp. arg...	[more]
KAG6606897.1	7.1e-192	99.43	Acyl-CoA--sterol O-acyltransferase 1, partial [Cucurbita argyrosperma subsp. sor...	[more]
XP_023523548.1	1.0e-190	98.58	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022998465.1	2.0e-186	96.88	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima]	[more]

BLAST of CmoCh01G002550 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51970.1	9.2e-65	42.31	acyl-CoA sterol acyl transferase 1	[more]
AT5G55350.1	1.2e-51	36.87	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55380.1	3.4e-51	40.72	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55370.1	4.5e-51	39.34	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55360.1	4.2e-49	39.43	MBOAT (membrane bound O-acyl transferase) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032805	Wax synthase domain	PFAM	PF13813	MBOAT_2	coord: 184..269 e-value: 4.6E-15 score: 55.6
None	No IPR available	PANTHER	PTHR31595:SF46	ACYL-COA--STEROL O-ACYLTRANSFERASE 1	coord: 3..341
IPR044851	Wax synthase	PANTHER	PTHR31595	LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED	coord: 3..341

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh01G002550.1	CmoCh01G002550.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008374	O-acyltransferase activity