CmoCh01G002410 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh01G002410
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptiontRNA-dihydrouridine synthase
LocationCmo_Chr01: 1084501 .. 1090143 (-)
RNA-Seq ExpressionCmoCh01G002410
SyntenyCmoCh01G002410
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTGTCGTGTTGAGCTTTTATTTTTATTTTTATTTTTATTTATTTATTCGTGTTTTTTCGTATGGTGAAAATTAAAAAAATTAAAAAAGAAAAGAAATCCGAGTATTTGAGGTGGCCTTAAACGCAACACCGAGTCAATCTTCGTGCTCAATCTTCTCCGCCGCTCAATCTTCGTCCGCATCAACTCCGCCGCACCGACCAGCGCGGAAACGGAGGTTGTCAGGTTCCCGCCGCTCCGGTAACCATATTTCCATGTAAACCTTAACATTCGTTTTGTATAAATCTGCAAATTAAGCATTTAACTTGTTTGCGTTTCTGCAATTGTGTCTGCGAACTGCCAATCGCTCTTATCATCGTAGCATTTCTTTGTTTAAATCATTGGTTCTGTAGAGGATGTTTGTTAAAAGGAGTTCTTAGCGGTTGTTCCGCCACTTTTAAAGCCTCTCAAGTTGCTCATGATCAGAAATTTCTGAGGAGCGGACTGTGTATGAATTGAGGGATGATGAAGTTTGCTGGATGTTCTATTACAGCTTCTTTGACTCCTATCCAGCCTTTCATTCAGAGAAGCCATCCTAGGCTGTCAATCAGTGTTTTACAGGAGAATTTATATAAACCACAGAAACTTGGAGAAGGTTCAGAAGCAGAAATGGTTGCTAGACGTTATTCCCCTCCTTTGTTTAGGTGATTGTTTTCCCATTATAGTTAATGATCCGTGTTCCTTAATGTTCATATCTTTTGATTGAAATTGACTTTTATTCACTTTCTCAGTGTTGCTCCTATGATGGATTGGACTGATAATCACTACAGAACTCTTGCTCGTATGATATCAAAGCATGCGTGGCTGTACACCGAGATGCTTGCTGCTGAAACAATTGTTTATCAGAAGGATAATCTTGTAAGTTTGCGGGAGAATCCTTTGGGGAATAGCTAATTATTCAATATTTCCAACAAATTTAGTAAGTAGGGTTGCTTTTGTTTATCAAAGATGTCATTTCCTTCATTTTCCACATTTTACTCGAGGTTTTCAAAATTGTGTGCAACATGTCGGACTAGTAATCAATTATTTGAGGTAGCCCTCCCTCACCCTCAGGCGTACTTGTTGAGGATGGTTGGGAGGGAGCCTCACATTGGCTGATTAAGGGAATGATAATTGTGTACTTTGTTCGAGGACTCCAGAGGATTGTTGTGATCCTCAAAAGTGTAGTCAAAAGTGACTCAAGTGTCGAACAAAGGGTGTACTTTGTTCGAGGACTCCAGAGGAATCGAGCTTCGAATAAGAGAAGGTTGTTTGAGGGCTCTTTAGACCTCAAGAGAGGCTCTATGGTGTACTTTGTTCAAAAGTGACTCAAGTGTCGAACAAATGGTGTACTTTGTTCGAGGACTCTAGAGGAGTCGAGCCTCGAATAAGAGGAGGTTGTTCGAGGACTTTTAGACCTCAAGAGAGGCTCTATGGTGTACTTTGTTCGAATGGAGGATTGTTGAGCATTGTTGGAAGGGAGTCCCACATCGACTGATTAAGGGAATAATCATCATTGGTATGAGGCCTTTTGGTGAAACCAAAAGAAACGTCATGAGAACTTATACTCAAAGTGGACAATATCATACCATTGTGGAGAGTCGTGGTTTCTAACAGTATTGCCTCCTTGTTAATACGTTGGGTTAGTAAATAATTATGTATGCCCCCTTCCTCCACTCTTATGATCTTATGAGAAGATTTGCCTCCAGTTACCCCAACAAAAGATCCTGGCCTTGATTCCTACAATATCCCACAACTTTCATGGTTTATAGTCTGAGTTGGACCAAGGATTTCTCTCATTTTACCAACATCTGGTCACCAAGAGTGACAGCGATGCTCTAATTAGATTGAACCTGTCAACTGTTGACTGCTATTTTTTACCTGTCATTATGAGGATGTAATTAATCTCCAAATTGGCCTGACCTCATTTCCTGTTTGTTTCTTTACCAGTCCAGGTTGGACTAGAGTTTTCTAATTTTTGATTGGTGAGAGTGAAGAATTGATGGGTGTTTTATTTCTCAAAGAAGAGTTACCATCTTATTGCTACATTTTGCAACTTGCATGTTTGAACTAGTAGGTGTGTGAGTCATATGAAGAATTTCTTCTCTTTCCATGAATCAAACTTGATTTGATTATGATATGCTTCGAATATCTATGTTTTTCTTGACAAATTCGGTATTCAAAGTTCCTTCTTTTCATGTAATCTTCATTTATTTTGCACAGGACAGATTTTTAGCATTTTCTCCAGAGCAACATCCCATTGTCCTGCAAATTGGTGGAAACAATCTTAATAACATTGCAAAAGCTGTTGAACTTGCCAAGCCTTACGGCTACGATGAAATTAATTTAAAGTCTGTATTTTACTCATCTTCTTCATATTTCTTTAGTTTCTACAATAAATTCTTAAATTCTAATATCTTTTTTTAAAATGTTTTTTAGCTGTGGATGTCCAAGTTCAAAGGTGGCTGGACATGGGTGCTTTGGTGTTCGCCTTATGCTGGATCCTAAGGTTTCTGGCTGTATTTTGATTTTGATCATCTTGTATATTTTATATTCTTCAGCCATGTGTCTGACGTAGGCTTTTCATGTTGACAAGGAAAGTTTGTTGGTGAAGCCATGTCAGTAATTGCTGCTAACACTGATGTCCCTGTAAGCGTGAAATGTCGAATTGGTGTCGATGACCATGATTCATATAATGAGCTTTGTAAGGTTTCTACTCTTTTTTCTCTTGTTAGAAAGCACCGTCTACTGTTTCTTTGCTTAGATAATCATCGTTATGTCTTGAGGTTAGTATTATATTATCGACGACTCAATCATAGGAACTCTATGATTTCTAAAGTTAAGTTCGTTTAACTAGAGTTATTCCATGTCGGGTGACTTTCCAAACATGTGAACGAAGACAAAACATGCAAATCCTAGTCCGGATGTTGGGTTTGGGTTGATACAAAATATTCTTTCCAAAGAGTTATTCTAGTTTGATGCTACATCATGTGGCTTCTTAGTTGTTGATTTCTCCGCTCATGTCAAGCACTTTTTGTTTCAGGTGATTTTGTTTACAAAGTTTCTTCTTCGTCGCCAACTAGGCATTTCATAATCCATTCCCGCAAGGCGTTACTCAACGGTATCAGCCCAGCTGAAAATCGAGAAATTCCTCCTTTGAAGTACGGCTTCTTAAGAACTTTATATAGGCCTAGAGAACTTGTCATTTGTGGTTCAAGTATTTTTACTAAGAACAGACTTTTGTAGTTCATAATATTGATGTGGTCACAGGTATGAATATTTCTATGCACTAATGCGCGACTTTCCAGACTTGAGATTCACAATAAATGGGGGCATTAAGACCGTTGATGAGGTGAACTTTCTGCTCAGCTAACTGGACCTAAAAGTATCAGAAATTGTGATTTGTTTTAAATGATAAGGTTTTTACATCATAATTTAATGTTCATTTATTTTGAATGAGCTTGTACAGGCTAATGCTGCTCTGAGACTAGGAGCTCATGGTGTAATGGTGGGCCGATCTGCTTACCAGAAGTATGTTTATGCACGTTTTGAAAATCAATATTCTATAAGAGTTGGTTATGATATACCTTAATTTGACTGATGGCTGTTGTTCATCCTCCATAGCCCTTGGCGTACCTTAGGACATGTCGACACTGCAATTTACGGTGCACCTAGCAGTGGTATCACACGACGTCAGGTGCAATCGTAAACTTGGCTGTGAGATTAGATTATCCTTCTGCTTGAAAGTTGACTGTTATCTCATTTAGTTGTAAACTTTTCTATCTTCTAGGTCCTTGAACAGTATCAAGTATATGGGGATTCTGTTTTGGGTACATATGGAAATAGACCAAGCATACGAGAGGTTGTGAAGGTAAGTAAGATCTTGTTGGTGGTTTGCAGCCTTAAACATAATTTTTGTCTGTTCAGTTATTTGGTCTAATTTTTTTGGTCATCTTATTGCTTTATACTGATCCAGAACCTTTTCAATTGTTGAGTTTAGGTGATTTTTTTTTTTTTTTTTTTATTTCTTTTTCGGCTTCCCCTCAAAGTAGTTTTTAGAATGCATACGCTATGAAGAGATTTCCACACTAAACAGGCATTGCTCTCTTTGGGGTTTCCCTTTCGAGCTTCCCCTCAAGATTTTCTCTCTTTGGGGTTTCCCTTTCGAGCTTCCCCTCAAGATTTTTAAAATGAGTCTACTAGGGAGAGGTTTTACCACCCATATAAAGAATGTTTCGTTCGTTTTCCGATGTAGAATTTCACAATCCACCCCTTCGAGGTTAGCATCCTCGCTTCCTTGTTGGCACTTGTTCCTTTCTCCAATCGATGTGGGACCCCCCAATCCACCCCTTTGAGACCCTACGTCCTCGTTAGCACACCGCCTCATGTCCACCCCCTTCGGGGCTTAGTCTCCTCACTGATACATCGCCTAGTGTCTCGCTCCAATCGATGTGGGACCCCCCAATCCACCCCTTCGAGACCCAACGTCCTCGCTAGCACACCGCCTCGTGTCCATCCCCTTCGGGGCTTAGTCTCCTCACTAGTACATCGCCTAGTATCTCGCTCTAATACCATTTGTAACAATTCAAACCTAGCATGAGCATATATTGTTTCTTTGGGCTTTTCGTTTTTAGAACACCTCTACTTGAGAGAGGTTTCCACACCCTTTCAAATAATGTTTCGTTCTCCTTGAGATTTCATGTTTACTTCTTATGAAATCGGTGTTTCTCTCTTAATCAATACTGACTGTATTCTAGCAAAATGCATCCCACCCCACACAAACACCCGGTATGGTAACTCGTCTCCTGTATCTGCTGCAGCCGTTGCTTCATCTTTTTTATACAGATCCTGGGAATGGTCCATGGAAGCGTAAAGCCGATGCCGCTTTTCAGCACTGCAAGGTATGTAAAATATGCTACAGAAGAACAACTTCGATTTTAGTTTTACGAAACGAAGTATTAAATTTTCAACGTTTTTCGCTTGCATTGTTATCATAGTTTTTATCCCTCAAAAGTCACTTCCTTTTGAACCTTGGCTGGATAGCTTCTAAGTGAACTGCCTATTGGACATTTGTAGACCATCAAGTCATTTTTCGATGAAACACTTGTAGCAATTCCAGATTATATATTGGATGCTCCTGTAGCAGAACCACCATCTGGACGTGAAGATCTTTTTGCCAATACACTTAGTTTGATGCCTCCTCCATATGAAGATAGAGAGCAAAAGATATTATTGGAGGCTTAATTTTGTATCATTGTTTAAGCCATGTATAACAGCATAACCTTTGAATACATCTCACTTATTCTTTGCAAATTCATCGATCCGGTAATCAAAGAAGTTGCCCTTGTAAACCGGTCATTCATCTTACTTATAAGCTTTTCTTGTCTCACTACTCGTTGCCATTTGTTTTCCAACCATCAAGAGTCTCAAGGTAAGAATCTGCTGGTCCAAAACTAGGGATTAGATGATGGTCCAGTCCTTTTGGCCGGAATCTTCAAATTCAAAGTTCAAACCCTAGAACGACAAATCGTTCGACAGAGGACACCTGTAATATCTCCGATTCAGATCGCCTAAAACCTACTGACAAAAATCGCCATCGACGTCCTAAATTTCGAACCAAAATTACC

mRNA sequence

TTTGTCGTGTTGAGCTTTTATTTTTATTTTTATTTTTATTTATTTATTCGTGTTTTTTCGTATGGTGAAAATTAAAAAAATTAAAAAAGAAAAGAAATCCGAGTATTTGAGGTGGCCTTAAACGCAACACCGAGTCAATCTTCGTGCTCAATCTTCTCCGCCGCTCAATCTTCGTCCGCATCAACTCCGCCGCACCGACCAGCGCGGAAACGGAGGTTGTCAGGTTCCCGCCGCTCCGGTAACCATATTTCCATAGGATGTTTGTTAAAAGGAGTTCTTAGCGGTTGTTCCGCCACTTTTAAAGCCTCTCAAGTTGCTCATGATCAGAAATTTCTGAGGAGCGGACTGTGTATGAATTGAGGGATGATGAAGTTTGCTGGATGTTCTATTACAGCTTCTTTGACTCCTATCCAGCCTTTCATTCAGAGAAGCCATCCTAGGCTGTCAATCAGTGTTTTACAGGAGAATTTATATAAACCACAGAAACTTGGAGAAGGTTCAGAAGCAGAAATGGTTGCTAGACGTTATTCCCCTCCTTTGTTTAGTGTTGCTCCTATGATGGATTGGACTGATAATCACTACAGAACTCTTGCTCGTATGATATCAAAGCATGCGTGGCTGTACACCGAGATGCTTGCTGCTGAAACAATTGTTTATCAGAAGGATAATCTTGACAGATTTTTAGCATTTTCTCCAGAGCAACATCCCATTGTCCTGCAAATTGGTGGAAACAATCTTAATAACATTGCAAAAGCTGTTGAACTTGCCAAGCCTTACGGCTACGATGAAATTAATTTAAACTGTGGATGTCCAAGTTCAAAGGTGGCTGGACATGGGTGCTTTGGTGTTCGCCTTATGCTGGATCCTAAGTTTGTTGGTGAAGCCATGTCAGTAATTGCTGCTAACACTGATGTCCCTGTAAGCGTGAAATGTCGAATTGGTGTCGATGACCATGATTCATATAATGAGCTTTGTGATTTTGTTTACAAAGTTTCTTCTTCGTCGCCAACTAGGCATTTCATAATCCATTCCCGCAAGGCGTTACTCAACGGTATCAGCCCAGCTGAAAATCGAGAAATTCCTCCTTTGAAGTATGAATATTTCTATGCACTAATGCGCGACTTTCCAGACTTGAGATTCACAATAAATGGGGGCATTAAGACCGTTGATGAGGCTAATGCTGCTCTGAGACTAGGAGCTCATGGTGTAATGGTGGGCCGATCTGCTTACCAGAACCCTTGGCGTACCTTAGGACATGTCGACACTGCAATTTACGGTGCACCTAGCAGTGGTATCACACGACGTCAGGTCCTTGAACAGTATCAAGTATATGGGGATTCTGTTTTGGGTACATATGGAAATAGACCAAGCATACGAGAGGTTGTGAAGCCGTTGCTTCATCTTTTTTATACAGATCCTGGGAATGGTCCATGGAAGCGTAAAGCCGATGCCGCTTTTCAGCACTGCAAGACCATCAAGTCATTTTTCGATGAAACACTTGTAGCAATTCCAGATTATATATTGGATGCTCCTGTAGCAGAACCACCATCTGGACGTGAAGATCTTTTTGCCAATACACTTAGTTTGATGCCTCCTCCATATGAAGATAGAGAGCAAAAGATATTATTGGAGGCTTAATTTTGTATCATTGTTTAAGCCATGTATAACAGCATAACCTTTGAATACATCTCACTTATTCTTTGCAAATTCATCGATCCGGTAATCAAAGAAGTTGCCCTTGTAAACCGGTCATTCATCTTACTTATAAGCTTTTCTTGTCTCACTACTCGTTGCCATTTGTTTTCCAACCATCAAGAGTCTCAAGGTAAGAATCTGCTGGTCCAAAACTAGGGATTAGATGATGGTCCAGTCCTTTTGGCCGGAATCTTCAAATTCAAAGTTCAAACCCTAGAACGACAAATCGTTCGACAGAGGACACCTGTAATATCTCCGATTCAGATCGCCTAAAACCTACTGACAAAAATCGCCATCGACGTCCTAAATTTCGAACCAAAATTACC

Coding sequence (CDS)

ATGATGAAGTTTGCTGGATGTTCTATTACAGCTTCTTTGACTCCTATCCAGCCTTTCATTCAGAGAAGCCATCCTAGGCTGTCAATCAGTGTTTTACAGGAGAATTTATATAAACCACAGAAACTTGGAGAAGGTTCAGAAGCAGAAATGGTTGCTAGACGTTATTCCCCTCCTTTGTTTAGTGTTGCTCCTATGATGGATTGGACTGATAATCACTACAGAACTCTTGCTCGTATGATATCAAAGCATGCGTGGCTGTACACCGAGATGCTTGCTGCTGAAACAATTGTTTATCAGAAGGATAATCTTGACAGATTTTTAGCATTTTCTCCAGAGCAACATCCCATTGTCCTGCAAATTGGTGGAAACAATCTTAATAACATTGCAAAAGCTGTTGAACTTGCCAAGCCTTACGGCTACGATGAAATTAATTTAAACTGTGGATGTCCAAGTTCAAAGGTGGCTGGACATGGGTGCTTTGGTGTTCGCCTTATGCTGGATCCTAAGTTTGTTGGTGAAGCCATGTCAGTAATTGCTGCTAACACTGATGTCCCTGTAAGCGTGAAATGTCGAATTGGTGTCGATGACCATGATTCATATAATGAGCTTTGTGATTTTGTTTACAAAGTTTCTTCTTCGTCGCCAACTAGGCATTTCATAATCCATTCCCGCAAGGCGTTACTCAACGGTATCAGCCCAGCTGAAAATCGAGAAATTCCTCCTTTGAAGTATGAATATTTCTATGCACTAATGCGCGACTTTCCAGACTTGAGATTCACAATAAATGGGGGCATTAAGACCGTTGATGAGGCTAATGCTGCTCTGAGACTAGGAGCTCATGGTGTAATGGTGGGCCGATCTGCTTACCAGAACCCTTGGCGTACCTTAGGACATGTCGACACTGCAATTTACGGTGCACCTAGCAGTGGTATCACACGACGTCAGGTCCTTGAACAGTATCAAGTATATGGGGATTCTGTTTTGGGTACATATGGAAATAGACCAAGCATACGAGAGGTTGTGAAGCCGTTGCTTCATCTTTTTTATACAGATCCTGGGAATGGTCCATGGAAGCGTAAAGCCGATGCCGCTTTTCAGCACTGCAAGACCATCAAGTCATTTTTCGATGAAACACTTGTAGCAATTCCAGATTATATATTGGATGCTCCTGTAGCAGAACCACCATCTGGACGTGAAGATCTTTTTGCCAATACACTTAGTTTGATGCCTCCTCCATATGAAGATAGAGAGCAAAAGATATTATTGGAGGCTTAA

Protein sequence

MMKFAGCSITASLTPIQPFIQRSHPRLSISVLQENLYKPQKLGEGSEAEMVARRYSPPLFSVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPEQHPIVLQIGGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKFVGEAMSVIAANTDVPVSVKCRIGVDDHDSYNELCDFVYKVSSSSPTRHFIIHSRKALLNGISPAENREIPPLKYEYFYALMRDFPDLRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQNPWRTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNRPSIREVVKPLLHLFYTDPGNGPWKRKADAAFQHCKTIKSFFDETLVAIPDYILDAPVAEPPSGREDLFANTLSLMPPPYEDREQKILLEA
Homology
[Close]
BLAST of CmoCh01G002410 vs. ExPASy Swiss-Prot
Match: Q7UBC5 (tRNA-dihydrouridine(20/20a) synthase OS=Shigella flexneri OX=623 GN=dusA PE=3 SV=3)

HSP 1 Score: 256.1 bits (653), Expect = 6.8e-67
Identity = 137/302 (45.36%), Postives = 187/302 (61.92%), Query Frame = 0 

Query: 60  FSVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPEQHPIVLQ 119
           FSVAPM+DWTD H R   R++S++  LYTEM+    I++ K +   +LA+S E+HP+ LQ
Sbjct: 22  FSVAPMLDWTDRHCRYFLRLLSRNTLLYTEMVTTGAIIHGKGD---YLAYSEEEHPVALQ 81

Query: 120 IGGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKFVGEAMSVIA 179
           +GG++   +A+  +LA+  GYDEINLN GCPS +V  +G FG  LM + + V + +  + 
Sbjct: 82  LGGSDPAALAQCAKLAEARGYDEINLNVGCPSDRVQ-NGMFGACLMGNAQLVADCVKAMR 141

Query: 180 ANTDVPVSVKCRIGVDDHDSYNELCDFVYKVSSSSPTRHFIIHSRKALLNGISPAENREI 239
               +PV+VK RIG+DD DSY  LCDF+  VS       FIIH+RKA L+G+SP ENREI
Sbjct: 142 DVVSIPVTVKTRIGIDDQDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREI 201

Query: 240 PPLKYEYFYALMRDFPDLRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQNPWRTLGHV 299
           PPL Y   Y L RDFP L  +INGGIK+++EA A L+    GVMVGR AYQNP   L  V
Sbjct: 202 PPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKAHLQ-HMDGVMVGREAYQNP-GILAAV 261

Query: 300 DTAIYGAPSSGITRRQVLEQYQVYGDSVL--GTYGNRPSIREVVKPLLHLFYTDPGNGPW 359
           D  I+G+  +      V+     Y +  L  GTY     +  +++ +L LF   PG   W
Sbjct: 262 DREIFGSSDTDADPVAVVRAMYPYIEHELSQGTY-----LGHIIRHMLGLFQGIPGARQW 312

[Close]
BLAST of CmoCh01G002410 vs. ExPASy Swiss-Prot
Match: Q8CWK7 (tRNA-dihydrouridine(20/20a) synthase OS=Vibrio vulnificus (strain CMCP6) OX=216895 GN=dusA PE=3 SV=2)

HSP 1 Score: 256.1 bits (653), Expect = 6.8e-67
Identity = 132/299 (44.15%), Postives = 185/299 (61.87%), Query Frame = 0 

Query: 61  SVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPEQHPIVLQI 120
           SVAPM+DWTD H R   R+++K   LYTEM+    I++ K +   FLA++ E+HP+ LQ+
Sbjct: 8   SVAPMLDWTDRHCRYFHRLMTKETLLYTEMITTGAIIHGKGD---FLAYNQEEHPVALQL 67

Query: 121 GGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKFVGEAMSVIAA 180
           GG+N  ++A   +LA   GYDE+NLN GCPS +V  +G FG  LM +P+ V + ++ +  
Sbjct: 68  GGSNPQDLATCAKLAAERGYDEVNLNVGCPSDRVQ-NGRFGACLMAEPQLVADCVAAMKE 127

Query: 181 NTDVPVSVKCRIGVDDHDSYNELCDFVYKVSSSSPTRHFIIHSRKALLNGISPAENREIP 240
             D+PV+VK RIG+DD DSY  L DFV  VS       F IH+RKA L+G+SP ENREIP
Sbjct: 128 VVDIPVTVKTRIGIDDQDSYEFLTDFVSIVSEKGGCEQFTIHARKAWLSGLSPKENREIP 187

Query: 241 PLKYEYFYALMRDFPDLRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQNPWRTLGHVD 300
           PL Y   Y L +DF  L   INGG+K+++EA   L+    GVM+GR AYQ+P+  L  VD
Sbjct: 188 PLDYPRAYQLKKDFSHLTIAINGGVKSLEEAKEHLQ-HLDGVMIGREAYQSPY-LLASVD 247

Query: 301 TAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNRPSIREVVKPLLHLFYTDPGNGPWKR 360
             ++G+ S    RRQ++E+   Y +  L    N   +  + + +L LF   PG   W+R
Sbjct: 248 QELFGSQSPIKKRRQIVEEMYPYIEQQL---ANGAYLGHMTRHMLGLFQNMPGARQWRR 297

[Close]
BLAST of CmoCh01G002410 vs. ExPASy Swiss-Prot
Match: Q8X5V6 (tRNA-dihydrouridine(20/20a) synthase OS=Escherichia coli O157:H7 OX=83334 GN=dusA PE=3 SV=3)

HSP 1 Score: 255.0 bits (650), Expect = 1.5e-66
Identity = 137/302 (45.36%), Postives = 186/302 (61.59%), Query Frame = 0 

Query: 60  FSVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPEQHPIVLQ 119
           FSVAPM+DWTD H R   R++S++  LYTEM+    I++ K +   +LA+S E+HP+ LQ
Sbjct: 28  FSVAPMLDWTDRHCRYFLRLLSRNTLLYTEMVTTGAIIHGKGD---YLAYSEEEHPVALQ 87

Query: 120 IGGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKFVGEAMSVIA 179
           +GG++   +A+  +LA+  GYDEINLN GCPS +V  +G FG  LM + + V + +  + 
Sbjct: 88  LGGSDPAALAQCAKLAEARGYDEINLNVGCPSDRVQ-NGMFGACLMGNAQLVADCVKAMR 147

Query: 180 ANTDVPVSVKCRIGVDDHDSYNELCDFVYKVSSSSPTRHFIIHSRKALLNGISPAENREI 239
               +PV+VK RIG+DD DSY  LCDF+  VS       FIIH+RKA L+G+SP ENREI
Sbjct: 148 DVVSIPVTVKTRIGIDDQDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREI 207

Query: 240 PPLKYEYFYALMRDFPDLRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQNPWRTLGHV 299
           PPL Y   Y L RDFP L  +INGGIK+++EA A L+    GVMVGR AYQNP   L  V
Sbjct: 208 PPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKAHLQ-HMDGVMVGREAYQNP-GILAAV 267

Query: 300 DTAIYGAPSSGITRRQVLEQYQVYGDSVL--GTYGNRPSIREVVKPLLHLFYTDPGNGPW 359
           D  I+G+  +      V+     Y +  L  GTY     +  + + +L LF   PG   W
Sbjct: 268 DREIFGSSDTDADPVAVVRAMYPYIERELSQGTY-----LGHITRHMLGLFQGIPGARQW 318

[Close]
BLAST of CmoCh01G002410 vs. ExPASy Swiss-Prot
Match: P32695 (tRNA-dihydrouridine(20/20a) synthase OS=Escherichia coli (strain K12) OX=83333 GN=dusA PE=1 SV=4)

HSP 1 Score: 255.0 bits (650), Expect = 1.5e-66
Identity = 137/302 (45.36%), Postives = 186/302 (61.59%), Query Frame = 0 

Query: 60  FSVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPEQHPIVLQ 119
           FSVAPM+DWTD H R   R++S++  LYTEM+    I++ K +   +LA+S E+HP+ LQ
Sbjct: 28  FSVAPMLDWTDRHCRYFLRLLSRNTLLYTEMVTTGAIIHGKGD---YLAYSEEEHPVALQ 87

Query: 120 IGGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKFVGEAMSVIA 179
           +GG++   +A+  +LA+  GYDEINLN GCPS +V  +G FG  LM + + V + +  + 
Sbjct: 88  LGGSDPAALAQCAKLAEARGYDEINLNVGCPSDRVQ-NGMFGACLMGNAQLVADCVKAMR 147

Query: 180 ANTDVPVSVKCRIGVDDHDSYNELCDFVYKVSSSSPTRHFIIHSRKALLNGISPAENREI 239
               +PV+VK RIG+DD DSY  LCDF+  VS       FIIH+RKA L+G+SP ENREI
Sbjct: 148 DVVSIPVTVKTRIGIDDQDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREI 207

Query: 240 PPLKYEYFYALMRDFPDLRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQNPWRTLGHV 299
           PPL Y   Y L RDFP L  +INGGIK+++EA A L+    GVMVGR AYQNP   L  V
Sbjct: 208 PPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKAHLQ-HMDGVMVGREAYQNP-GILAAV 267

Query: 300 DTAIYGAPSSGITRRQVLEQYQVYGDSVL--GTYGNRPSIREVVKPLLHLFYTDPGNGPW 359
           D  I+G+  +      V+     Y +  L  GTY     +  + + +L LF   PG   W
Sbjct: 268 DREIFGSSDTDADPVAVVRAMYPYIERELSQGTY-----LGHITRHMLGLFQGIPGARQW 318

[Close]
BLAST of CmoCh01G002410 vs. ExPASy Swiss-Prot
Match: Q8FB30 (tRNA-dihydrouridine(20/20a) synthase OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) OX=199310 GN=dusA PE=3 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 2.0e-66
Identity = 136/302 (45.03%), Postives = 186/302 (61.59%), Query Frame = 0 

Query: 60  FSVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPEQHPIVLQ 119
           FS+APM+DWTD H R   R++S++  LYTEM+    I++ K +   +LA+S E+HP+ LQ
Sbjct: 14  FSIAPMLDWTDRHCRYFLRLLSRNTLLYTEMVTTGAIIHGKGD---YLAYSEEEHPVALQ 73

Query: 120 IGGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKFVGEAMSVIA 179
           +GG++   +A+  +LA+  GYDEINLN GCPS +V  +G FG  LM + + V + +  + 
Sbjct: 74  LGGSDPAALAQCAKLAEARGYDEINLNVGCPSDRVQ-NGMFGACLMGNAQLVADCVKAMR 133

Query: 180 ANTDVPVSVKCRIGVDDHDSYNELCDFVYKVSSSSPTRHFIIHSRKALLNGISPAENREI 239
               +PV+VK RIG+DD DSY  LCDF+  VS       FIIH+RKA L+G+SP ENREI
Sbjct: 134 DVVSIPVTVKTRIGIDDQDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREI 193

Query: 240 PPLKYEYFYALMRDFPDLRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQNPWRTLGHV 299
           PPL Y   Y L RDFP L  +INGGIK+++EA A L+    GVMVGR AYQNP   L  V
Sbjct: 194 PPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKAHLQ-HMDGVMVGREAYQNP-GILAAV 253

Query: 300 DTAIYGAPSSGITRRQVLEQYQVYGDSVL--GTYGNRPSIREVVKPLLHLFYTDPGNGPW 359
           D  I+G+  +      V+     Y +  L  GTY     +  + + +L LF   PG   W
Sbjct: 254 DREIFGSSDTDADPVAVVRAMYPYIERELSQGTY-----LGHITRHMLGLFQGIPGARQW 304

[Close]
BLAST of CmoCh01G002410 vs. ExPASy TrEMBL
Match: A0A6J1GBU8 (uncharacterized protein LOC111452746 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111452746 PE=4 SV=1)

HSP 1 Score: 879.8 bits (2272), Expect = 4.6e-252
Identity = 424/424 (100.00%), Postives = 424/424 (100.00%), Query Frame = 0 

Query: 1   MMKFAGCSITASLTPIQPFIQRSHPRLSISVLQENLYKPQKLGEGSEAEMVARRYSPPLF 60
           MMKFAGCSITASLTPIQPFIQRSHPRLSISVLQENLYKPQKLGEGSEAEMVARRYSPPLF
Sbjct: 1   MMKFAGCSITASLTPIQPFIQRSHPRLSISVLQENLYKPQKLGEGSEAEMVARRYSPPLF 60

Query: 61  SVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPEQHPIVLQI 120
           SVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPEQHPIVLQI
Sbjct: 61  SVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPEQHPIVLQI 120

Query: 121 GGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKFVGEAMSVIAA 180
           GGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKFVGEAMSVIAA
Sbjct: 121 GGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKFVGEAMSVIAA 180

Query: 181 NTDVPVSVKCRIGVDDHDSYNELCDFVYKVSSSSPTRHFIIHSRKALLNGISPAENREIP 240
           NTDVPVSVKCRIGVDDHDSYNELCDFVYKVSSSSPTRHFIIHSRKALLNGISPAENREIP
Sbjct: 181 NTDVPVSVKCRIGVDDHDSYNELCDFVYKVSSSSPTRHFIIHSRKALLNGISPAENREIP 240

Query: 241 PLKYEYFYALMRDFPDLRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQNPWRTLGHVD 300
           PLKYEYFYALMRDFPDLRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQNPWRTLGHVD
Sbjct: 241 PLKYEYFYALMRDFPDLRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQNPWRTLGHVD 300

Query: 301 TAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNRPSIREVVKPLLHLFYTDPGNGPWKRK 360
           TAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNRPSIREVVKPLLHLFYTDPGNGPWKRK
Sbjct: 301 TAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNRPSIREVVKPLLHLFYTDPGNGPWKRK 360

Query: 361 ADAAFQHCKTIKSFFDETLVAIPDYILDAPVAEPPSGREDLFANTLSLMPPPYEDREQKI 420
           ADAAFQHCKTIKSFFDETLVAIPDYILDAPVAEPPSGREDLFANTLSLMPPPYEDREQKI
Sbjct: 361 ADAAFQHCKTIKSFFDETLVAIPDYILDAPVAEPPSGREDLFANTLSLMPPPYEDREQKI 420

Query: 421 LLEA 425
           LLEA
Sbjct: 421 LLEA 424

[Close]
BLAST of CmoCh01G002410 vs. ExPASy TrEMBL
Match: A0A6J1KEC0 (uncharacterized protein LOC111493074 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111493074 PE=4 SV=1)

HSP 1 Score: 864.0 bits (2231), Expect = 2.6e-247
Identity = 415/424 (97.88%), Postives = 417/424 (98.35%), Query Frame = 0 

Query: 1   MMKFAGCSITASLTPIQPFIQRSHPRLSISVLQENLYKPQKLGEGSEAEMVARRYSPPLF 60
           MMKFAGCSITASLTPIQPFIQRSHPRLSI VLQENLYKPQKLGEGSEAEMVARRYSPPLF
Sbjct: 1   MMKFAGCSITASLTPIQPFIQRSHPRLSIGVLQENLYKPQKLGEGSEAEMVARRYSPPLF 60

Query: 61  SVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPEQHPIVLQI 120
           SVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPEQHPIVLQI
Sbjct: 61  SVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPEQHPIVLQI 120

Query: 121 GGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKFVGEAMSVIAA 180
           GGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKFVGEAMSVIAA
Sbjct: 121 GGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKFVGEAMSVIAA 180

Query: 181 NTDVPVSVKCRIGVDDHDSYNELCDFVYKVSSSSPTRHFIIHSRKALLNGISPAENREIP 240
           NTDVPVSVKCRIGVDDHDSYNELCDFVYKVSS SPTRHFIIHSRKALLNGISPAENREIP
Sbjct: 181 NTDVPVSVKCRIGVDDHDSYNELCDFVYKVSSLSPTRHFIIHSRKALLNGISPAENREIP 240

Query: 241 PLKYEYFYALMRDFPDLRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQNPWRTLGHVD 300
           PLKYEYFYALMRDFPDLRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQNPWRTLGHVD
Sbjct: 241 PLKYEYFYALMRDFPDLRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQNPWRTLGHVD 300

Query: 301 TAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNRPSIREVVKPLLHLFYTDPGNGPWKRK 360
           TAIYGAPSSGITRRQVLEQYQVYGDS+LG YGNRPSIREVVKPLLHLFYTDPGNGPWKRK
Sbjct: 301 TAIYGAPSSGITRRQVLEQYQVYGDSILGKYGNRPSIREVVKPLLHLFYTDPGNGPWKRK 360

Query: 361 ADAAFQHCKTIKSFFDETLVAIPDYILDAPVAEPPSGREDLFANTLSLMPPPYEDREQKI 420
           ADAAFQHCKTIKSFFDETLVAIPDYILDAPVAE P GREDLF NTLSLMPPPYED EQK+
Sbjct: 361 ADAAFQHCKTIKSFFDETLVAIPDYILDAPVAESPPGREDLFVNTLSLMPPPYEDTEQKV 420

Query: 421 LLEA 425
           LLEA
Sbjct: 421 LLEA 424

[Close]
BLAST of CmoCh01G002410 vs. ExPASy TrEMBL
Match: A0A6J1DED7 (uncharacterized protein LOC111020285 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111020285 PE=4 SV=1)

HSP 1 Score: 809.3 bits (2089), Expect = 7.6e-231
Identity = 385/431 (89.33%), Postives = 407/431 (94.43%), Query Frame = 0 

Query: 1   MMKFAGCSITASLTPIQPFIQRSHPRLSISVLQENLYKP-------QKLGEGSEAEMVAR 60
           MMKFAGCSITASLTPIQ  IQ+SH RLS ++LQENL KP       QKL +G EAEMVAR
Sbjct: 1   MMKFAGCSITASLTPIQSVIQKSHCRLSSNILQENLSKPSGWSSSNQKLRQGPEAEMVAR 60

Query: 61  RYSPPLFSVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPEQ 120
           RYSPP FSVAPMMDWTDNHYRTLAR+ISKHAWLYTEMLAAETIVYQKDNLDRFLAFSP+Q
Sbjct: 61  RYSPPWFSVAPMMDWTDNHYRTLARLISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPDQ 120

Query: 121 HPIVLQIGGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKFVGE 180
           HPIVLQ+GGNNLNNIAKA ELA  YGYDEIN NCGCPS+KVAGHGCFGVRLMLDPKFVGE
Sbjct: 121 HPIVLQLGGNNLNNIAKATELANAYGYDEINFNCGCPSAKVAGHGCFGVRLMLDPKFVGE 180

Query: 181 AMSVIAANTDVPVSVKCRIGVDDHDSYNELCDFVYKVSSSSPTRHFIIHSRKALLNGISP 240
           AMSVIAANTDVPVSVKCRIGVDDHDSYNELCDF+YKVSS SPTRHFIIHSRKALLNGISP
Sbjct: 181 AMSVIAANTDVPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTRHFIIHSRKALLNGISP 240

Query: 241 AENREIPPLKYEYFYALMRDFPDLRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQNPW 300
           AENR IPPLKYEYFYALMRDFPDLRFTINGGI +VDE NAAL+LGAHGVM+GR+AYQNPW
Sbjct: 241 AENRSIPPLKYEYFYALMRDFPDLRFTINGGINSVDEVNAALKLGAHGVMMGRAAYQNPW 300

Query: 301 RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNRPSIREVVKPLLHLFYTDPG 360
           RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGN+P+IREVVKPLLHLFY+DPG
Sbjct: 301 RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIREVVKPLLHLFYSDPG 360

Query: 361 NGPWKRKADAAFQHCKTIKSFFDETLVAIPDYILDAPVAEPPSGREDLFANTLSLMPPPY 420
           NGPWKRKADAAFQHCKTIKSFF+ETLVA+PDYILDAPVAEPP GREDLFANTLSL+PPPY
Sbjct: 361 NGPWKRKADAAFQHCKTIKSFFEETLVAVPDYILDAPVAEPPPGREDLFANTLSLLPPPY 420

Query: 421 EDREQKILLEA 425
           ED+EQK++LEA
Sbjct: 421 EDKEQKVVLEA 431

[Close]
BLAST of CmoCh01G002410 vs. ExPASy TrEMBL
Match: A0A6J1F1C8 (uncharacterized protein LOC111441480 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111441480 PE=4 SV=1)

HSP 1 Score: 800.8 bits (2067), Expect = 2.7e-228
Identity = 381/428 (89.02%), Postives = 403/428 (94.16%), Query Frame = 0 

Query: 1   MMKFAGCSITASLTPIQPFIQRSHPRLSISVLQENLYKP-------QKLGEGSEAEMVAR 60
           MMKFAGCS++ASL+P+Q FIQ+SHPRLS S+LQ+NLYKP       Q L +  EAEMVA 
Sbjct: 1   MMKFAGCSVSASLSPVQSFIQKSHPRLSSSILQKNLYKPSSWFSGNQILQQSPEAEMVAG 60

Query: 61  RYSPPLFSVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPEQ 120
           RYSPP FSVAPMMD TDNHYRTLAR+ISKHAWLY+EMLAAETIVYQKDNLDRFLAFSP+Q
Sbjct: 61  RYSPPWFSVAPMMDCTDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFSPDQ 120

Query: 121 HPIVLQIGGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKFVGE 180
           HPIVLQIGGNNLNNIAKAVELAKPYGYDEINLNCGCPS KVAGHGCFG RLMLDPKFVGE
Sbjct: 121 HPIVLQIGGNNLNNIAKAVELAKPYGYDEINLNCGCPSPKVAGHGCFGARLMLDPKFVGE 180

Query: 181 AMSVIAANTDVPVSVKCRIGVDDHDSYNELCDFVYKVSSSSPTRHFIIHSRKALLNGISP 240
           AMSVIAANT+ PVSVKCRIGVDDHDSYNELCDF+YKVSS SPT+HFIIHSRKALLNGISP
Sbjct: 181 AMSVIAANTNAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNGISP 240

Query: 241 AENREIPPLKYEYFYALMRDFPDLRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQNPW 300
           AENR IPPLKYEYFYALMRDFPDLRFTINGGI TVDE NAALRLGAHGVM+GR+AYQNPW
Sbjct: 241 AENRNIPPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVMMGRAAYQNPW 300

Query: 301 RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNRPSIREVVKPLLHLFYTDPG 360
           RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGN+P+IR+VVKPLLHLFYTDPG
Sbjct: 301 RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVKPLLHLFYTDPG 360

Query: 361 NGPWKRKADAAFQHCKTIKSFFDETLVAIPDYILDAPVAEPPSGREDLFANTLSLMPPPY 420
           NGPWKRKAD AFQHCKTIKSFF+ETLVAIPDYILDAPVAEPPSGREDLFANTL LMPPPY
Sbjct: 361 NGPWKRKADFAFQHCKTIKSFFEETLVAIPDYILDAPVAEPPSGREDLFANTLRLMPPPY 420

Query: 421 EDREQKIL 422
           EDREQK++
Sbjct: 421 EDREQKVV 428

[Close]
BLAST of CmoCh01G002410 vs. ExPASy TrEMBL
Match: A0A6J1F233 (uncharacterized protein LOC111441480 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111441480 PE=4 SV=1)

HSP 1 Score: 800.0 bits (2065), Expect = 4.6e-228
Identity = 382/431 (88.63%), Postives = 404/431 (93.74%), Query Frame = 0 

Query: 1   MMKFAGCSITASLTPIQPFIQRSHPRLSISVLQENLYKP-------QKLGEGSEAEMVAR 60
           MMKFAGCS++ASL+P+Q FIQ+SHPRLS S+LQ+NLYKP       Q L +  EAEMVA 
Sbjct: 1   MMKFAGCSVSASLSPVQSFIQKSHPRLSSSILQKNLYKPSSWFSGNQILQQSPEAEMVAG 60

Query: 61  RYSPPLFSVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPEQ 120
           RYSPP FSVAPMMD TDNHYRTLAR+ISKHAWLY+EMLAAETIVYQKDNLDRFLAFSP+Q
Sbjct: 61  RYSPPWFSVAPMMDCTDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFSPDQ 120

Query: 121 HPIVLQIGGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKFVGE 180
           HPIVLQIGGNNLNNIAKAVELAKPYGYDEINLNCGCPS KVAGHGCFG RLMLDPKFVGE
Sbjct: 121 HPIVLQIGGNNLNNIAKAVELAKPYGYDEINLNCGCPSPKVAGHGCFGARLMLDPKFVGE 180

Query: 181 AMSVIAANTDVPVSVKCRIGVDDHDSYNELCDFVYKVSSSSPTRHFIIHSRKALLNGISP 240
           AMSVIAANT+ PVSVKCRIGVDDHDSYNELCDF+YKVSS SPT+HFIIHSRKALLNGISP
Sbjct: 181 AMSVIAANTNAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNGISP 240

Query: 241 AENREIPPLKYEYFYALMRDFPDLRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQNPW 300
           AENR IPPLKYEYFYALMRDFPDLRFTINGGI TVDE NAALRLGAHGVM+GR+AYQNPW
Sbjct: 241 AENRNIPPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVMMGRAAYQNPW 300

Query: 301 RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNRPSIREVVKPLLHLFYTDPG 360
           RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGN+P+IR+VVKPLLHLFYTDPG
Sbjct: 301 RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVKPLLHLFYTDPG 360

Query: 361 NGPWKRKADAAFQHCKTIKSFFDETLVAIPDYILDAPVAEPPSGREDLFANTLSLMPPPY 420
           NGPWKRKAD AFQHCKTIKSFF+ETLVAIPDYILDAPVAEPPSGREDLFANTL LMPPPY
Sbjct: 361 NGPWKRKADFAFQHCKTIKSFFEETLVAIPDYILDAPVAEPPSGREDLFANTLRLMPPPY 420

Query: 421 EDREQKILLEA 425
           EDREQK + +A
Sbjct: 421 EDREQKSMDDA 431

[Close]
BLAST of CmoCh01G002410 vs. NCBI nr
Match: XP_022949368.1 (uncharacterized protein LOC111452746 isoform X1 [Cucurbita moschata] >XP_022949369.1 uncharacterized protein LOC111452746 isoform X1 [Cucurbita moschata] >XP_022949370.1 uncharacterized protein LOC111452746 isoform X1 [Cucurbita moschata] >XP_022949371.1 uncharacterized protein LOC111452746 isoform X1 [Cucurbita moschata] >XP_022949373.1 uncharacterized protein LOC111452746 isoform X1 [Cucurbita moschata])

HSP 1 Score: 879.8 bits (2272), Expect = 9.5e-252
Identity = 424/424 (100.00%), Postives = 424/424 (100.00%), Query Frame = 0 

Query: 1   MMKFAGCSITASLTPIQPFIQRSHPRLSISVLQENLYKPQKLGEGSEAEMVARRYSPPLF 60
           MMKFAGCSITASLTPIQPFIQRSHPRLSISVLQENLYKPQKLGEGSEAEMVARRYSPPLF
Sbjct: 1   MMKFAGCSITASLTPIQPFIQRSHPRLSISVLQENLYKPQKLGEGSEAEMVARRYSPPLF 60

Query: 61  SVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPEQHPIVLQI 120
           SVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPEQHPIVLQI
Sbjct: 61  SVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPEQHPIVLQI 120

Query: 121 GGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKFVGEAMSVIAA 180
           GGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKFVGEAMSVIAA
Sbjct: 121 GGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKFVGEAMSVIAA 180

Query: 181 NTDVPVSVKCRIGVDDHDSYNELCDFVYKVSSSSPTRHFIIHSRKALLNGISPAENREIP 240
           NTDVPVSVKCRIGVDDHDSYNELCDFVYKVSSSSPTRHFIIHSRKALLNGISPAENREIP
Sbjct: 181 NTDVPVSVKCRIGVDDHDSYNELCDFVYKVSSSSPTRHFIIHSRKALLNGISPAENREIP 240

Query: 241 PLKYEYFYALMRDFPDLRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQNPWRTLGHVD 300
           PLKYEYFYALMRDFPDLRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQNPWRTLGHVD
Sbjct: 241 PLKYEYFYALMRDFPDLRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQNPWRTLGHVD 300

Query: 301 TAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNRPSIREVVKPLLHLFYTDPGNGPWKRK 360
           TAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNRPSIREVVKPLLHLFYTDPGNGPWKRK
Sbjct: 301 TAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNRPSIREVVKPLLHLFYTDPGNGPWKRK 360

Query: 361 ADAAFQHCKTIKSFFDETLVAIPDYILDAPVAEPPSGREDLFANTLSLMPPPYEDREQKI 420
           ADAAFQHCKTIKSFFDETLVAIPDYILDAPVAEPPSGREDLFANTLSLMPPPYEDREQKI
Sbjct: 361 ADAAFQHCKTIKSFFDETLVAIPDYILDAPVAEPPSGREDLFANTLSLMPPPYEDREQKI 420

Query: 421 LLEA 425
           LLEA
Sbjct: 421 LLEA 424

[Close]
BLAST of CmoCh01G002410 vs. NCBI nr
Match: KAG6606881.1 (hypothetical protein SDJN03_00223, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 878.2 bits (2268), Expect = 2.8e-251
Identity = 423/424 (99.76%), Postives = 423/424 (99.76%), Query Frame = 0 

Query: 1   MMKFAGCSITASLTPIQPFIQRSHPRLSISVLQENLYKPQKLGEGSEAEMVARRYSPPLF 60
           MMKFAGCSITASLTPIQPFIQRSHPRLSISVLQENLYKPQKLGEGSEAEMVARRYSPPLF
Sbjct: 1   MMKFAGCSITASLTPIQPFIQRSHPRLSISVLQENLYKPQKLGEGSEAEMVARRYSPPLF 60

Query: 61  SVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPEQHPIVLQI 120
           SVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPEQHPIVLQI
Sbjct: 61  SVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPEQHPIVLQI 120

Query: 121 GGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKFVGEAMSVIAA 180
           GGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKFVGEAMSVIAA
Sbjct: 121 GGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKFVGEAMSVIAA 180

Query: 181 NTDVPVSVKCRIGVDDHDSYNELCDFVYKVSSSSPTRHFIIHSRKALLNGISPAENREIP 240
           NTDVPVSVKCRIGVDDHDSYNELCDFVYKVSSSSPTRHFIIHSRKALLNGISPAENREIP
Sbjct: 181 NTDVPVSVKCRIGVDDHDSYNELCDFVYKVSSSSPTRHFIIHSRKALLNGISPAENREIP 240

Query: 241 PLKYEYFYALMRDFPDLRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQNPWRTLGHVD 300
           PLKYEYFYALMRDFPDLRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQNPWRTLGHVD
Sbjct: 241 PLKYEYFYALMRDFPDLRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQNPWRTLGHVD 300

Query: 301 TAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNRPSIREVVKPLLHLFYTDPGNGPWKRK 360
           TAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNRPSIREVVKPLLHLFYTDPGNGPWKRK
Sbjct: 301 TAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNRPSIREVVKPLLHLFYTDPGNGPWKRK 360

Query: 361 ADAAFQHCKTIKSFFDETLVAIPDYILDAPVAEPPSGREDLFANTLSLMPPPYEDREQKI 420
           ADAAFQHCKTIKSFFDETLVAIPDYILDAPVAEPPSGREDLFANTL LMPPPYEDREQKI
Sbjct: 361 ADAAFQHCKTIKSFFDETLVAIPDYILDAPVAEPPSGREDLFANTLGLMPPPYEDREQKI 420

Query: 421 LLEA 425
           LLEA
Sbjct: 421 LLEA 424

[Close]
BLAST of CmoCh01G002410 vs. NCBI nr
Match: XP_023525441.1 (uncharacterized protein LOC111789048 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023525442.1 uncharacterized protein LOC111789048 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023525443.1 uncharacterized protein LOC111789048 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023525444.1 uncharacterized protein LOC111789048 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023525445.1 uncharacterized protein LOC111789048 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 875.5 bits (2261), Expect = 1.8e-250
Identity = 421/424 (99.29%), Postives = 422/424 (99.53%), Query Frame = 0 

Query: 1   MMKFAGCSITASLTPIQPFIQRSHPRLSISVLQENLYKPQKLGEGSEAEMVARRYSPPLF 60
           MMKFAGCSITASLTPIQPFIQRSHPRLSI VLQENLYKPQKLGEGSEAEMVARRYSPPLF
Sbjct: 1   MMKFAGCSITASLTPIQPFIQRSHPRLSIGVLQENLYKPQKLGEGSEAEMVARRYSPPLF 60

Query: 61  SVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPEQHPIVLQI 120
           SVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPEQHPIVLQI
Sbjct: 61  SVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPEQHPIVLQI 120

Query: 121 GGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKFVGEAMSVIAA 180
           GGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKFVGEAMSVIAA
Sbjct: 121 GGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKFVGEAMSVIAA 180

Query: 181 NTDVPVSVKCRIGVDDHDSYNELCDFVYKVSSSSPTRHFIIHSRKALLNGISPAENREIP 240
           NTDVPVSVKCRIGVDDHDSYNELCDFVYKVSS SPTRHFIIHSRKALLNGISPAENREIP
Sbjct: 181 NTDVPVSVKCRIGVDDHDSYNELCDFVYKVSSLSPTRHFIIHSRKALLNGISPAENREIP 240

Query: 241 PLKYEYFYALMRDFPDLRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQNPWRTLGHVD 300
           PLKYEYFYALMRDFPDLRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQNPWRTLGHVD
Sbjct: 241 PLKYEYFYALMRDFPDLRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQNPWRTLGHVD 300

Query: 301 TAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNRPSIREVVKPLLHLFYTDPGNGPWKRK 360
           TAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNRPSIREVVKPLLHLFYTDPGNGPWKRK
Sbjct: 301 TAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNRPSIREVVKPLLHLFYTDPGNGPWKRK 360

Query: 361 ADAAFQHCKTIKSFFDETLVAIPDYILDAPVAEPPSGREDLFANTLSLMPPPYEDREQKI 420
           ADAAFQHCKTIKSFFDETLVAIPDYILDAPVAEPPSGREDLFANTLSLMPPPYEDREQK+
Sbjct: 361 ADAAFQHCKTIKSFFDETLVAIPDYILDAPVAEPPSGREDLFANTLSLMPPPYEDREQKV 420

Query: 421 LLEA 425
           LLEA
Sbjct: 421 LLEA 424

[Close]
BLAST of CmoCh01G002410 vs. NCBI nr
Match: XP_022998438.1 (uncharacterized protein LOC111493074 isoform X1 [Cucurbita maxima] >XP_022998439.1 uncharacterized protein LOC111493074 isoform X1 [Cucurbita maxima] >XP_022998440.1 uncharacterized protein LOC111493074 isoform X1 [Cucurbita maxima] >XP_022998441.1 uncharacterized protein LOC111493074 isoform X1 [Cucurbita maxima] >XP_022998442.1 uncharacterized protein LOC111493074 isoform X1 [Cucurbita maxima])

HSP 1 Score: 864.0 bits (2231), Expect = 5.4e-247
Identity = 415/424 (97.88%), Postives = 417/424 (98.35%), Query Frame = 0 

Query: 1   MMKFAGCSITASLTPIQPFIQRSHPRLSISVLQENLYKPQKLGEGSEAEMVARRYSPPLF 60
           MMKFAGCSITASLTPIQPFIQRSHPRLSI VLQENLYKPQKLGEGSEAEMVARRYSPPLF
Sbjct: 1   MMKFAGCSITASLTPIQPFIQRSHPRLSIGVLQENLYKPQKLGEGSEAEMVARRYSPPLF 60

Query: 61  SVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPEQHPIVLQI 120
           SVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPEQHPIVLQI
Sbjct: 61  SVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPEQHPIVLQI 120

Query: 121 GGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKFVGEAMSVIAA 180
           GGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKFVGEAMSVIAA
Sbjct: 121 GGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKFVGEAMSVIAA 180

Query: 181 NTDVPVSVKCRIGVDDHDSYNELCDFVYKVSSSSPTRHFIIHSRKALLNGISPAENREIP 240
           NTDVPVSVKCRIGVDDHDSYNELCDFVYKVSS SPTRHFIIHSRKALLNGISPAENREIP
Sbjct: 181 NTDVPVSVKCRIGVDDHDSYNELCDFVYKVSSLSPTRHFIIHSRKALLNGISPAENREIP 240

Query: 241 PLKYEYFYALMRDFPDLRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQNPWRTLGHVD 300
           PLKYEYFYALMRDFPDLRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQNPWRTLGHVD
Sbjct: 241 PLKYEYFYALMRDFPDLRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQNPWRTLGHVD 300

Query: 301 TAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNRPSIREVVKPLLHLFYTDPGNGPWKRK 360
           TAIYGAPSSGITRRQVLEQYQVYGDS+LG YGNRPSIREVVKPLLHLFYTDPGNGPWKRK
Sbjct: 301 TAIYGAPSSGITRRQVLEQYQVYGDSILGKYGNRPSIREVVKPLLHLFYTDPGNGPWKRK 360

Query: 361 ADAAFQHCKTIKSFFDETLVAIPDYILDAPVAEPPSGREDLFANTLSLMPPPYEDREQKI 420
           ADAAFQHCKTIKSFFDETLVAIPDYILDAPVAE P GREDLF NTLSLMPPPYED EQK+
Sbjct: 361 ADAAFQHCKTIKSFFDETLVAIPDYILDAPVAESPPGREDLFVNTLSLMPPPYEDTEQKV 420

Query: 421 LLEA 425
           LLEA
Sbjct: 421 LLEA 424

[Close]
BLAST of CmoCh01G002410 vs. NCBI nr
Match: KAG7036588.1 (dusA [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 844.3 bits (2180), Expect = 4.4e-241
Identity = 412/430 (95.81%), Postives = 414/430 (96.28%), Query Frame = 0 

Query: 1   MMKFAGCSITASLTPIQPFIQRSHPRLSISVLQENLYKPQKLGEGSEAEMVARRYSPPLF 60
           MMKFAGCSITASLTPIQPFIQRSHPRLSISVLQENLYKPQKLGEGSEAEMVARRYSPPLF
Sbjct: 1   MMKFAGCSITASLTPIQPFIQRSHPRLSISVLQENLYKPQKLGEGSEAEMVARRYSPPLF 60

Query: 61  SVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPEQHPIVLQI 120
           SVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPEQHPIVLQI
Sbjct: 61  SVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPEQHPIVLQI 120

Query: 121 GGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKFVGEAMSVIAA 180
           GGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKFVGEAMSVIAA
Sbjct: 121 GGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKFVGEAMSVIAA 180

Query: 181 NTDVPVSVKCRIGVDDHDSYNELCDFVYKVSSSSPTRHFIIHSRKALLNGISPAENREIP 240
           NTDVPVSVKCRIGVDDHDSYNELCDFVYKVSSSSPTRHFIIHSRKALLNGISPAENREIP
Sbjct: 181 NTDVPVSVKCRIGVDDHDSYNELCDFVYKVSSSSPTRHFIIHSRKALLNGISPAENREIP 240

Query: 241 PLKYEYFYALMRD------FPDLRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQNPWR 300
           PLKY +   L R         +LRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQNPW 
Sbjct: 241 PLKYGFLRTLYRPRELVICGSNLRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQNPWH 300

Query: 301 TLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNRPSIREVVKPLLHLFYTDPGN 360
           TLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNRPSIREVVKPLLHLFYTDPGN
Sbjct: 301 TLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNRPSIREVVKPLLHLFYTDPGN 360

Query: 361 GPWKRKADAAFQHCKTIKSFFDETLVAIPDYILDAPVAEPPSGREDLFANTLSLMPPPYE 420
           GPWKRKADAAF HCKTIKSFFDETLVAIPDYILDAPVAEPPSGREDLFANTLSLMPPPYE
Sbjct: 361 GPWKRKADAAFHHCKTIKSFFDETLVAIPDYILDAPVAEPPSGREDLFANTLSLMPPPYE 420

Query: 421 DREQKILLEA 425
           DREQKILLEA
Sbjct: 421 DREQKILLEA 430

[Close]
BLAST of CmoCh01G002410 vs. TAIR 10
Match: AT3G63510.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 595.9 bits (1535), Expect = 2.6e-170
Identity = 275/392 (70.15%), Postives = 335/392 (85.46%), Query Frame = 0 

Query: 32  LQENLYKPQKLGEGSEAEMVARRYSPPLFSVAPMMDWTDNHYRTLARMISKHAWLYTEML 91
           + E  +K   L   +     +  Y PP FSVAPMMDWTDNHYRTLAR+I+KHAWLYTEM+
Sbjct: 26  VSEMFFKSPMLKPTARFSSTSSPYLPPSFSVAPMMDWTDNHYRTLARLITKHAWLYTEMI 85

Query: 92  AAETIVYQKDNLDRFLAFSPEQHPIVLQIGGNNLNNIAKAVELAKPYGYDEINLNCGCPS 151
           AAET+V+Q+ NLDRFLAFSP+QHPIVLQ+GG+N+ N+AKA +L+  YGYDEINLNCGCPS
Sbjct: 86  AAETLVHQQTNLDRFLAFSPQQHPIVLQLGGSNVENLAKAAKLSDAYGYDEINLNCGCPS 145

Query: 152 SKVAGHGCFGVRLMLDPKFVGEAMSVIAANTDVPVSVKCRIGVDDHDSYNELCDFVYKVS 211
            KVAGHGCFGV LML PK VGEAMS IAANT+VPV+VKCRIGVD+HDSY+ELCDF+YKVS
Sbjct: 146 PKVAGHGCFGVSLMLKPKLVGEAMSAIAANTNVPVTVKCRIGVDNHDSYDELCDFIYKVS 205

Query: 212 SSSPTRHFIIHSRKALLNGISPAENREIPPLKYEYFYALMRDFPDLRFTINGGIKTVDEA 271
           + SPTRHFI+HSRKALL GISPA+NR IPPLKYEY+YAL+RDFPDLRFTINGGI +V + 
Sbjct: 206 TLSPTRHFIVHSRKALLGGISPADNRRIPPLKYEYYYALVRDFPDLRFTINGGITSVSKV 265

Query: 272 NAALRLGAHGVMVGRSAYQNPWRTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTY 331
           NAAL+ GAHGVMVGR+AY NPW+TLG VDTA+YG PSSG+TRRQVLEQYQVYGDSVLGT+
Sbjct: 266 NAALKEGAHGVMVGRAAYNNPWQTLGQVDTAVYGVPSSGLTRRQVLEQYQVYGDSVLGTH 325

Query: 332 GN-RPSIREVVKPLLHLFYTDPGNGPWKRKADAAFQHCKTIKSFFDETLVAIPDYILDAP 391
           GN RP++R++VKPLL+LF+++ GN  WKR+ADAAF+ C+++ S  +E+L AIPD +LD+P
Sbjct: 326 GNGRPNVRDLVKPLLNLFHSENGNSLWKRRADAAFKECRSVGSLLEESLRAIPDCVLDSP 385

Query: 392 VA-EPPSGREDLFANTLSLMPPPYEDREQKIL 422
           ++  P SG ED+FA+  +++PPPYE  E+ IL
Sbjct: 386 ISGSPESGDEDVFADVHNVLPPPYEAGEEIIL 417

[Close]
BLAST of CmoCh01G002410 vs. TAIR 10
Match: AT5G47970.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 589.0 bits (1517), Expect = 3.1e-168
Identity = 268/372 (72.04%), Postives = 318/372 (85.48%), Query Frame = 0 

Query: 51  VARRYSPPLFSVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFS 110
           V+  YSPPLFS+APMM WTDNHYRTLAR+I+KHAWLYTEMLAAETIVYQ+DNLD FLAFS
Sbjct: 3   VSEAYSPPLFSIAPMMGWTDNHYRTLARLITKHAWLYTEMLAAETIVYQEDNLDSFLAFS 62

Query: 111 PEQHPIVLQIGGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKF 170
           P+QHPIVLQIGG NL N+AKA  LA  Y YDEIN NCGCPS KV+G GCFG  LMLDPKF
Sbjct: 63  PDQHPIVLQIGGRNLENLAKATRLANAYAYDEINFNCGCPSPKVSGRGCFGALLMLDPKF 122

Query: 171 VGEAMSVIAANTDVPVSVKCRIGVDDHDSYNELCDFVYKVSSSSPTRHFIIHSRKALLNG 230
           VGEAMSVIAANT+  V+VKCRIGVDDHDSYNELCDF++ VSS SPT+HFIIHSRKALL+G
Sbjct: 123 VGEAMSVIAANTNAAVTVKCRIGVDDHDSYNELCDFIHIVSSLSPTKHFIIHSRKALLSG 182

Query: 231 ISPAENREIPPLKYEYFYALMRDFPDLRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQ 290
           +SP++NR IPPLKYE+F+AL+RDFPDL+FTINGGI +V EA+AALR GAHGVM+GR+ Y 
Sbjct: 183 LSPSDNRRIPPLKYEFFFALLRDFPDLKFTINGGINSVVEADAALRSGAHGVMLGRAVYY 242

Query: 291 NPWRTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYG-NRPSIREVVKPLLHLFY 350
           NPW  LGHVDT +YG+PSSGITRRQVLE+Y++YG+SVLG YG  RP++R++V+PL++LF+
Sbjct: 243 NPWHILGHVDTVVYGSPSSGITRRQVLEKYKLYGESVLGKYGKGRPNLRDIVRPLINLFH 302

Query: 351 TDPGNGPWKRKADAAFQHCKTIKSFFDETLVAIPDYILDAPVAEPPSGREDLFANTLSLM 410
           ++ GNG WKR+ DAA  HC T++SF DE L AIPDY+LD+   +  +GREDLFA+   L+
Sbjct: 303 SESGNGQWKRRTDAALLHCTTLQSFLDEVLPAIPDYVLDSSAVKEATGREDLFADVQRLL 362

Query: 411 PPPYEDREQKIL 422
           PPPYE    K L
Sbjct: 363 PPPYEKESFKAL 374

[Close]
BLAST of CmoCh01G002410 vs. TAIR 10
Match: AT5G47970.2 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 589.0 bits (1517), Expect = 3.1e-168
Identity = 268/372 (72.04%), Postives = 318/372 (85.48%), Query Frame = 0 

Query: 51  VARRYSPPLFSVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFS 110
           V+  YSPPLFS+APMM WTDNHYRTLAR+I+KHAWLYTEMLAAETIVYQ+DNLD FLAFS
Sbjct: 3   VSEAYSPPLFSIAPMMGWTDNHYRTLARLITKHAWLYTEMLAAETIVYQEDNLDSFLAFS 62

Query: 111 PEQHPIVLQIGGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKF 170
           P+QHPIVLQIGG NL N+AKA  LA  Y YDEIN NCGCPS KV+G GCFG  LMLDPKF
Sbjct: 63  PDQHPIVLQIGGRNLENLAKATRLANAYAYDEINFNCGCPSPKVSGRGCFGALLMLDPKF 122

Query: 171 VGEAMSVIAANTDVPVSVKCRIGVDDHDSYNELCDFVYKVSSSSPTRHFIIHSRKALLNG 230
           VGEAMSVIAANT+  V+VKCRIGVDDHDSYNELCDF++ VSS SPT+HFIIHSRKALL+G
Sbjct: 123 VGEAMSVIAANTNAAVTVKCRIGVDDHDSYNELCDFIHIVSSLSPTKHFIIHSRKALLSG 182

Query: 231 ISPAENREIPPLKYEYFYALMRDFPDLRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQ 290
           +SP++NR IPPLKYE+F+AL+RDFPDL+FTINGGI +V EA+AALR GAHGVM+GR+ Y 
Sbjct: 183 LSPSDNRRIPPLKYEFFFALLRDFPDLKFTINGGINSVVEADAALRSGAHGVMLGRAVYY 242

Query: 291 NPWRTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYG-NRPSIREVVKPLLHLFY 350
           NPW  LGHVDT +YG+PSSGITRRQVLE+Y++YG+SVLG YG  RP++R++V+PL++LF+
Sbjct: 243 NPWHILGHVDTVVYGSPSSGITRRQVLEKYKLYGESVLGKYGKGRPNLRDIVRPLINLFH 302

Query: 351 TDPGNGPWKRKADAAFQHCKTIKSFFDETLVAIPDYILDAPVAEPPSGREDLFANTLSLM 410
           ++ GNG WKR+ DAA  HC T++SF DE L AIPDY+LD+   +  +GREDLFA+   L+
Sbjct: 303 SESGNGQWKRRTDAALLHCTTLQSFLDEVLPAIPDYVLDSSAVKEATGREDLFADVQRLL 362

Query: 411 PPPYEDREQKIL 422
           PPPYE    K L
Sbjct: 363 PPPYEKESFKAL 374

[Close]
BLAST of CmoCh01G002410 vs. TAIR 10
Match: AT3G63510.2 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 566.6 bits (1459), Expect = 1.7e-161
Identity = 263/368 (71.47%), Postives = 322/368 (87.50%), Query Frame = 0 

Query: 56  SPPLFSVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPEQHP 115
           SPPL     ++  +DNHYRTLAR+I+KHAWLYTEM+AAET+V+Q+ NLDRFLAFSP+QHP
Sbjct: 18  SPPLRLPIFLLP-SDNHYRTLARLITKHAWLYTEMIAAETLVHQQTNLDRFLAFSPQQHP 77

Query: 116 IVLQIGGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKFVGEAM 175
           IVLQ+GG+N+ N+AKA +L+  YGYDEINLNCGCPS KVAGHGCFGV LML PK VGEAM
Sbjct: 78  IVLQLGGSNVENLAKAAKLSDAYGYDEINLNCGCPSPKVAGHGCFGVSLMLKPKLVGEAM 137

Query: 176 SVIAANTDVPVSVKCRIGVDDHDSYNELCDFVYKVSSSSPTRHFIIHSRKALLNGISPAE 235
           S IAANT+VPV+VKCRIGVD+HDSY+ELCDF+YKVS+ SPTRHFI+HSRKALL GISPA+
Sbjct: 138 SAIAANTNVPVTVKCRIGVDNHDSYDELCDFIYKVSTLSPTRHFIVHSRKALLGGISPAD 197

Query: 236 NREIPPLKYEYFYALMRDFPDLRFTINGGIKTVDEANAALRLGAHGVMVGRSAYQNPWRT 295
           NR IPPLKYEY+YAL+RDFPDLRFTINGGI +V + NAAL+ GAHGVMVGR+AY NPW+T
Sbjct: 198 NRRIPPLKYEYYYALVRDFPDLRFTINGGITSVSKVNAALKEGAHGVMVGRAAYNNPWQT 257

Query: 296 LGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGN-RPSIREVVKPLLHLFYTDPGN 355
           LG VDTA+YG PSSG+TRRQVLEQYQVYGDSVLGT+GN RP++R++VKPLL+LF+++ GN
Sbjct: 258 LGQVDTAVYGVPSSGLTRRQVLEQYQVYGDSVLGTHGNGRPNVRDLVKPLLNLFHSENGN 317

Query: 356 GPWKRKADAAFQHCKTIKSFFDETLVAIPDYILDAPVA-EPPSGREDLFANTLSLMPPPY 415
             WKR+ADAAF+ C+++ S  +E+L AIPD +LD+P++  P SG ED+FA+  +++PPPY
Sbjct: 318 SLWKRRADAAFKECRSVGSLLEESLRAIPDCVLDSPISGSPESGDEDVFADVHNVLPPPY 377

Query: 416 EDREQKIL 422
           E  E+ IL
Sbjct: 378 EAGEEIIL 384

[Close]
BLAST of CmoCh01G002410 vs. TAIR 10
Match: AT5G67220.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 67.0 bits (162), Expect = 4.1e-11
Identity = 55/244 (22.54%), Postives = 107/244 (43.85%), Query Frame = 0 

Query: 53  RRYSPPLFSVAPMMDWTDNHYRTLARMISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPE 112
           ++   P + VAPM+D ++  +R L +     A  YT ML +      +   ++      E
Sbjct: 83  KKLGRPKYIVAPMVDNSELPFRLLCQKYGAQA-AYTPMLHSRIFTETEKYRNQEFTTCKE 142

Query: 113 QHPIVLQIGGNNLNNIAKAVELAKPYGYDEINLNCGCPSSKVAGHGCFGVRLMLDPKFVG 172
             P+ +Q   N+ + + +A +  +PY  D +++N GCP  ++A  G +G  LM +   V 
Sbjct: 143 DRPLFVQFCANDPDTLLEAAKRVEPY-CDYVDINLGCP-QRIARRGNYGAFLMDNLPLVK 202

Query: 173 EAMSVIAANTDVPVSVKCRIGVDDHDSYNELCDFVYKVSSSSPTRHFIIHSRKALLNGIS 232
             +  +A N +VPVS K RI  +  D+         K+   +      +H R        
Sbjct: 203 SLVEKLAQNLNVPVSCKIRIFPNLEDTLK-----YAKMLEDAGCSLLAVHGR-------- 262

Query: 233 PAENREIPPLKYEYFYALMRDFPD-LRFTI--NGGIKTVDEA-NAALRLGAHGVMVGRSA 292
                E    K+   ++ +++  + +R  +  NG ++ +++  N     G  GV+   + 
Sbjct: 263 --TRDEKDGKKFRADWSAIKEVKNAMRIPVLANGNVRCIEDVDNCIKETGVEGVLSAETL 308

The following BLAST results are available for this feature:
BLAST of CmoCh01G002410 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q7UBC56.8e-6745.36tRNA-dihydrouridine(20/20a) synthase OS=Shigella flexneri OX=623 GN=dusA PE=3 SV... [more]
Q8CWK76.8e-6744.15tRNA-dihydrouridine(20/20a) synthase OS=Vibrio vulnificus (strain CMCP6) OX=2168... [more]
Q8X5V61.5e-6645.36tRNA-dihydrouridine(20/20a) synthase OS=Escherichia coli O157:H7 OX=83334 GN=dus... [more]
P326951.5e-6645.36tRNA-dihydrouridine(20/20a) synthase OS=Escherichia coli (strain K12) OX=83333 G... [more]
Q8FB302.0e-6645.03tRNA-dihydrouridine(20/20a) synthase OS=Escherichia coli O6:H1 (strain CFT073 / ... [more]
BLAST of CmoCh01G002410 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1GBU84.6e-252100.00uncharacterized protein LOC111452746 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1KEC02.6e-24797.88uncharacterized protein LOC111493074 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1DED77.6e-23189.33uncharacterized protein LOC111020285 isoform X1 OS=Momordica charantia OX=3673 G... [more]
A0A6J1F1C82.7e-22889.02uncharacterized protein LOC111441480 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1F2334.6e-22888.63uncharacterized protein LOC111441480 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
BLAST of CmoCh01G002410 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022949368.19.5e-252100.00uncharacterized protein LOC111452746 isoform X1 [Cucurbita moschata] >XP_0229493... [more]
KAG6606881.12.8e-25199.76hypothetical protein SDJN03_00223, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023525441.11.8e-25099.29uncharacterized protein LOC111789048 isoform X1 [Cucurbita pepo subsp. pepo] >XP... [more]
XP_022998438.15.4e-24797.88uncharacterized protein LOC111493074 isoform X1 [Cucurbita maxima] >XP_022998439... [more]
KAG7036588.14.4e-24195.81dusA [Cucurbita argyrosperma subsp. argyrosperma][more]
BLAST of CmoCh01G002410 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G63510.12.6e-17070.15FMN-linked oxidoreductases superfamily protein [more]
AT5G47970.13.1e-16872.04Aldolase-type TIM barrel family protein [more]
AT5G47970.23.1e-16872.04Aldolase-type TIM barrel family protein [more]
AT3G63510.21.7e-16171.47FMN-linked oxidoreductases superfamily protein [more]
AT5G67220.14.1e-1122.54FMN-linked oxidoreductases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR035587DUS-like, FMN-binding domainPFAMPF01207Duscoord: 62..372
e-value: 2.2E-60
score: 204.4
IPR035587DUS-like, FMN-binding domainCDDcd02801DUS_like_FMNcoord: 60..293
e-value: 1.21505E-79
score: 243.941
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 57..297
e-value: 7.0E-71
score: 240.5
IPR004653tRNA-dihydrouridine(20/20a) synthasePANTHERPTHR42907FMN-LINKED OXIDOREDUCTASES SUPERFAMILY PROTEINcoord: 50..420
NoneNo IPR availablePANTHERPTHR42907:SF2TRNA-DIHYDROURIDINE SYNTHASEcoord: 50..420
NoneNo IPR availableSUPERFAMILY51395FMN-linked oxidoreductasescoord: 60..360

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh01G002410.1CmoCh01G002410.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0002943 tRNA dihydrouridine synthesis
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0017150 tRNA dihydrouridine synthase activity
molecular_function GO:0003824 catalytic activity