CmoCh01G001520 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh01G001520
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	(1->3)-beta-glucan endohydrolase
Location	Cmo_Chr01: 652388 .. 653744 (+)
RNA-Seq Expression	CmoCh01G001520
Synteny	CmoCh01G001520

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCTACTTCCCATCACCATTTGGCGGTCTTGACACCAGATCTTTGTAGCAACTGCTTACTCTCTTCCACGATGACAGTAACTTTAGTATGTCTTAGCTTTAGTTTTACGAATGTATTTACCGAAAAGAGAACTCTTAGTTGTCAGAATTAGAGTTTAGAATCTGCAGCTTTTGTTGTTTTTGCTTACCGAACTTCTTTTGACTGTAGAATGTTACTGTTAATCAACCTTCTAATTTAACTTCGGGTGAAACATGTTCTTCTGTTTCTTACTGCTGCTGTTTTTGCTCTTTTCTTTCTCAACGATCCAATAATAACTTCCATTCTGTAACAGCCAAACTCCACCACCACTAATAGATATTGTCCGCTTTGGTCCGTTACGTATTGTCATCAGTCTCATGACTTTAACGTGCATCTACTAGGAAGAGGTTTCCACATTCTTAAAAGGAAGACTTTGTTCTCCTCTCCATTACCCGAAGAAGTAGAACATCATGCTGAAACAAAGCAAGATGGATATTCCAAACCCCATCCATGAAACATGACAGAGAAAATGGAGGTTTTTCCAAGAAAAGCAAGAGATGAAAAGAAAGGGAAGAATCTATACTATGCCTCCTTTAAAGAACTCCATAGGAATAACGAATCTTCTTCCATCTCATCAAAATTTGAATCATGTTTAGACAAAGGCTAAGGAATCTGAGAGAAGGAAAGGATTGCATCATTCAAATGATGTTGTGCTCCTATTTTTTGGCCAACCAAGTTAGTTTTTCAATACCCATTAGCTACAAAAGGAACTCTTTTTCTTTCTACTTTTGCCTCCCCACAAACAAAATGGCTACTCATTTCCTCCCTCCTCCTGCAGTTTCAAGGAAGATGTTGTGGCATTTATGAACCCTCTTTTGCAATTCTTCTCCCTCATTGCCTCTCCTTTTTTCATTAATGCATACCCATTCCTTGCTTACAACAATGACCCTCAGCATATTGACATCAACTATGCACTCTTCAACAACAACAACCGCATTTTCGACCCCAAAACGTTCGACGCTCAAGTCGATGCAGCTTATGCTGCTCTCCACAATGTCGGCTTCCCCAACATGCCAGTCATCGTTTCCGAGACTGGCTGGGCTTCTCATGGGGACGAGAATGAAGCCGGTGCGACGTTGGAAAATGCAAGGACATACAATAAAAATCTACTTAAGAAGTTGATGAAGAAGAAGGGAACTCCTTACCAACCCAAAACTGTTGTCAAGGCTTACGTTTTTGCTCTCTTCAACGAGAACTCAAAGCCCAGGCCTACGTTCGAGAGGAACTTCGGCCTCTTCAAGCCTGATGCCAGCATTGCATATGATATCAGCGGTTGA

mRNA sequence

ATGCCTACTTCCCATCACCATTTGGCGGTCTTGACACCAGATCTTTGTAGCAACTGCTTACTCTCTTCCACGATGACAGTAACTTTAGTATGTCTTAGCTTTAGTTTTACGAATGTATTTACCGAAAAGAGAACTCTTAGTTATGTTGTGGCATTTATGAACCCTCTTTTGCAATTCTTCTCCCTCATTGCCTCTCCTTTTTTCATTAATGCATACCCATTCCTTGCTTACAACAATGACCCTCAGCATATTGACATCAACTATGCACTCTTCAACAACAACAACCGCATTTTCGACCCCAAAACGTTCGACGCTCAAGTCGATGCAGCTTATGCTGCTCTCCACAATGTCGGCTTCCCCAACATGCCAGTCATCGTTTCCGAGACTGGCTGGGCTTCTCATGGGGACGAGAATGAAGCCGGTGCGACGTTGGAAAATGCAAGGACATACAATAAAAATCTACTTAAGAAGTTGATGAAGAAGAAGGGAACTCCTTACCAACCCAAAACTGTTGTCAAGGCTTACGTTTTTGCTCTCTTCAACGAGAACTCAAAGCCCAGGCCTACGTTCGAGAGGAACTTCGGCCTCTTCAAGCCTGATGCCAGCATTGCATATGATATCAGCGGTTGA

Coding sequence (CDS)

ATGCCTACTTCCCATCACCATTTGGCGGTCTTGACACCAGATCTTTGTAGCAACTGCTTACTCTCTTCCACGATGACAGTAACTTTAGTATGTCTTAGCTTTAGTTTTACGAATGTATTTACCGAAAAGAGAACTCTTAGTTATGTTGTGGCATTTATGAACCCTCTTTTGCAATTCTTCTCCCTCATTGCCTCTCCTTTTTTCATTAATGCATACCCATTCCTTGCTTACAACAATGACCCTCAGCATATTGACATCAACTATGCACTCTTCAACAACAACAACCGCATTTTCGACCCCAAAACGTTCGACGCTCAAGTCGATGCAGCTTATGCTGCTCTCCACAATGTCGGCTTCCCCAACATGCCAGTCATCGTTTCCGAGACTGGCTGGGCTTCTCATGGGGACGAGAATGAAGCCGGTGCGACGTTGGAAAATGCAAGGACATACAATAAAAATCTACTTAAGAAGTTGATGAAGAAGAAGGGAACTCCTTACCAACCCAAAACTGTTGTCAAGGCTTACGTTTTTGCTCTCTTCAACGAGAACTCAAAGCCCAGGCCTACGTTCGAGAGGAACTTCGGCCTCTTCAAGCCTGATGCCAGCATTGCATATGATATCAGCGGTTGA

Protein sequence

MPTSHHHLAVLTPDLCSNCLLSSTMTVTLVCLSFSFTNVFTEKRTLSYVVAFMNPLLQFFSLIASPFFINAYPFLAYNNDPQHIDINYALFNNNNRIFDPKTFDAQVDAAYAALHNVGFPNMPVIVSETGWASHGDENEAGATLENARTYNKNLLKKLMKKKGTPYQPKTVVKAYVFALFNENSKPRPTFERNFGLFKPDASIAYDISG

Homology

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BLAST of CmoCh01G001520 vs. ExPASy Swiss-Prot
Match: Q9ZQG9 (Glucan endo-1,3-beta-glucosidase 14 OS=Arabidopsis thaliana OX=3702 GN=At2g27500 PE=2 SV=2)

HSP 1 Score: 162.9 bits (411), Expect = 3.8e-39
Identity = 82/165 (49.70%), Postives = 106/165 (64.24%), Query Frame = 0


Query: 50  VAFMNPLLQFFSLIASPFFINAYPFLAYNNDPQHIDINYALFNNNNRIFDPKT---FD-- 109
           + ++ PLL F S I SPF INAYPF AY + P+ + + Y LF  N  + DP T   +D  
Sbjct: 182 IQYLQPLLDFHSQIESPFLINAYPFFAYKDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNM 241

Query: 110 --AQVDAAYAALHNVGFPNMPVIVSETGWASHGDENEAGATLENARTYNKNLLKKLMKKK 169
             AQVDA Y+A+  +G  ++ V +SETGW S GDENE GA+ ENA  YN NLLK + ++K
Sbjct: 242 LFAQVDALYSAIKTLGHTDIEVRISETGWPSKGDENEIGASPENAALYNGNLLKLIQQRK 301

Query: 170 GTPYQPKTVVKAYVFALFNENSKPRPTFERNFGLFKPDASIAYDI 208
           GTP +    +  YVFALFNEN KP P  ERN+GLF PD    Y++
Sbjct: 302 GTPAKQSVPIDVYVFALFNENLKPGPVSERNYGLFYPDGKPVYNV 346

[Close]

BLAST of CmoCh01G001520 vs. ExPASy Swiss-Prot
Match: Q8L868 (Glucan endo-1,3-beta-glucosidase 11 OS=Arabidopsis thaliana OX=3702 GN=At1g32860 PE=2 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 1.5e-38
Identity = 83/164 (50.61%), Postives = 108/164 (65.85%), Query Frame = 0


Query: 53  MNPLLQFFSLIASPFFINAYPFLAYNNDPQHIDINYALFNNNNRIFDPKT---FD----A 112
           + P+L+F     SPF INAYPF AY  +P+ + +++ LF  N  I DP T   +D    A
Sbjct: 184 LTPILEFHRKTDSPFLINAYPFFAYKGNPKEVPLDFVLFQPNQGIVDPATGFHYDNMLFA 243

Query: 113 QVDAAYAALHNVGFPNMPVIVSETGWASHGDENEAGATLENARTYNKNLLKKLM--KKKG 172
           Q+DA Y+AL   GF ++ V +SETGW S GD++E GAT ENA+ YN NL+K +M  KK  
Sbjct: 244 QIDAVYSALAAAGFKSLRVEISETGWPSKGDDDEVGATPENAKRYNGNLIKMMMSGKKTK 303

Query: 173 TPYQPKTVVKAYVFALFNENSKPRPTFERNFGLFKPDASIAYDI 208
           TP +P   +  YVFALFNEN KP PT ERN+GLFKPD + AY +
Sbjct: 304 TPLKPNNDLSIYVFALFNENLKPGPTSERNYGLFKPDGTQAYSL 347

[Close]

BLAST of CmoCh01G001520 vs. ExPASy Swiss-Prot
Match: Q9FHX5 (Glucan endo-1,3-beta-glucosidase 10 OS=Arabidopsis thaliana OX=3702 GN=At5g42100 PE=2 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 2.3e-36
Identity = 80/165 (48.48%), Postives = 105/165 (63.64%), Query Frame = 0


Query: 53  MNPLLQFFSLIASPFFINAYPFLAYNNDPQHIDINYALFNNNNRIFDPKT---FD----A 112
           + P+L F     SP  INAYPF AY  +P+H+ +++ LF  N    DP +   +D    A
Sbjct: 182 LTPILDFHVKTGSPILINAYPFFAYEENPKHVSLDFVLFQPNQGFTDPGSNFHYDNMLFA 241

Query: 113 QVDAAYAALHNVG--FPNMPVIVSETGWASHGDENEAGATLENARTYNKNLLKKLMKKK- 172
           QVDA Y AL  VG  +  +P++VSETGW S+GD  E GAT +NAR YN NL+K +M KK 
Sbjct: 242 QVDAVYHALDAVGISYKKVPIVVSETGWPSNGDPQEVGATCDNARKYNGNLIKMMMSKKM 301

Query: 173 GTPYQPKTVVKAYVFALFNENSKPRPTFERNFGLFKPDASIAYDI 208
            TP +P+  +  +VFALFNEN KP PT ERN+GLF PD +  Y +
Sbjct: 302 RTPIRPECDLTIFVFALFNENMKPGPTSERNYGLFNPDGTPVYSL 346

[Close]

BLAST of CmoCh01G001520 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 153.7 bits (387), Expect = 2.3e-36
Identity = 78/159 (49.06%), Postives = 102/159 (64.15%), Query Frame = 0


Query: 56  LLQFFSLIASPFFINAYPFLAYNNDPQHIDINYALFNNNNRIFDPKT-------FDAQVD 115
           +LQF S   SPF IN YPF AY +DP+   + + LF  N    D KT       FDAQVD
Sbjct: 185 ILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVD 244

Query: 116 AAYAALHNVGFPNMPVIVSETGWASHGDENEAGATLENARTYNKNLLKKLMKKKGTPYQP 175
           A ++AL ++GF  + ++V+ETGWAS GD NE GA+++NA+ YN NL+  L    GTP  P
Sbjct: 245 AVHSALKSMGFEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMP 304

Query: 176 KTVVKAYVFALFNENSKPRPTFERNFGLFKPDASIAYDI 208
              V  Y+FAL++EN KP P+ ER FGLFK D S+ YD+
Sbjct: 305 GKPVDTYIFALYDENLKPGPSSERAFGLFKTDLSMVYDV 343

[Close]

BLAST of CmoCh01G001520 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 1.7e-31
Identity = 66/164 (40.24%), Postives = 102/164 (62.20%), Query Frame = 0


Query: 52  FMNPLLQFFSLIASPFFINAYPFLAYNNDPQHIDINYALFNNNNRIFDPKT-------FD 111
           F+ P+L+F +   SPF I+ YP+ AY + P ++ ++Y LF +++ + DP T       FD
Sbjct: 181 FLRPMLEFLAENKSPFMIDLYPYYAYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFD 240

Query: 112 AQVDAAYAALHNVGFPNMPVIVSETGWASHGDENE-AGATLENARTYNKNLLKKLMKKKG 171
           AQVDA Y AL  + F  + ++V+ETGW + G   E A A+ +NA TYN N+++ ++  +G
Sbjct: 241 AQVDALYYALTALNFRTIKIMVTETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQG 300

Query: 172 TPYQPKTVVKAYVFALFNENSKPRPTFERNFGLFKPDASIAYDI 208
           TP +P   +  Y+F+LFNEN K     ERN+GLF PD +  Y +
Sbjct: 301 TPAKPGEAMNVYIFSLFNENRKAGLDSERNWGLFYPDQTSVYQL 344

[Close]

BLAST of CmoCh01G001520 vs. ExPASy TrEMBL
Match: A0A0A0KTE7 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_4G005530 PE=3 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 4.5e-67
Identity = 129/166 (77.71%), Postives = 141/166 (84.94%), Query Frame = 0


Query: 49  VVAFMNPLLQFFSLIASPFFINAYPFLAYNNDPQHIDINYALFNNNNRIFDPKT------ 108
           +V FM PLLQFFS I SPFFINAYPFLAY NDP+HIDINYALF  N  IFD KT      
Sbjct: 180 IVPFMKPLLQFFSFIGSPFFINAYPFLAYKNDPEHIDINYALFKKNKGIFDAKTNLHYDN 239

Query: 109 -FDAQVDAAYAALHNVGFPNMPVIVSETGWASHGDENEAGATLENARTYNKNLLKKLMKK 168
            F+AQVDAAYAAL  VG+P MPVIVSETGWASHGDENEAGAT++NARTYN+NL KKLMKK
Sbjct: 240 MFEAQVDAAYAALEKVGYPKMPVIVSETGWASHGDENEAGATMKNARTYNRNLRKKLMKK 299

Query: 169 KGTPYQPKTVVKAYVFALFNENSKPRPTFERNFGLFKPDASIAYDI 208
           KGTP++PK VV+AY+FALFNENSKP PT ERNFGLFKPD SI+YDI
Sbjct: 300 KGTPFRPKMVVRAYIFALFNENSKPGPTSERNFGLFKPDGSISYDI 345

[Close]

BLAST of CmoCh01G001520 vs. ExPASy TrEMBL
Match: A0A5A7TSV0 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001780 PE=3 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 7.7e-67
Identity = 129/166 (77.71%), Postives = 140/166 (84.34%), Query Frame = 0


Query: 49  VVAFMNPLLQFFSLIASPFFINAYPFLAYNNDPQHIDINYALFNNNNRIFDPKT------ 108
           +V FM PLLQFFS I SPFFINAYPFLAY NDP+HIDINYALF  N  IFD KT      
Sbjct: 538 IVPFMKPLLQFFSFIGSPFFINAYPFLAYKNDPEHIDINYALFKKNKGIFDAKTNLHYDN 597

Query: 109 -FDAQVDAAYAALHNVGFPNMPVIVSETGWASHGDENEAGATLENARTYNKNLLKKLMKK 168
            F+AQVDAAYAAL   G+P MPVIVSETGWASHGDENEAGATL+NARTYN+NL KKLMKK
Sbjct: 598 MFEAQVDAAYAALEKAGYPKMPVIVSETGWASHGDENEAGATLKNARTYNRNLRKKLMKK 657

Query: 169 KGTPYQPKTVVKAYVFALFNENSKPRPTFERNFGLFKPDASIAYDI 208
           KGTP++PK VV+AY+FALFNENSKP PT ERNFGLFKPD SI+YDI
Sbjct: 658 KGTPFRPKMVVRAYIFALFNENSKPGPTSERNFGLFKPDGSISYDI 703

[Close]

BLAST of CmoCh01G001520 vs. ExPASy TrEMBL
Match: A0A1S3BYW7 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103494697 PE=3 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 7.7e-67
Identity = 129/166 (77.71%), Postives = 140/166 (84.34%), Query Frame = 0


Query: 49  VVAFMNPLLQFFSLIASPFFINAYPFLAYNNDPQHIDINYALFNNNNRIFDPKT------ 108
           +V FM PLLQFFS I SPFFINAYPFLAY NDP+HIDINYALF  N  IFD KT      
Sbjct: 179 IVPFMKPLLQFFSFIGSPFFINAYPFLAYKNDPEHIDINYALFKKNKGIFDAKTNLHYDN 238

Query: 109 -FDAQVDAAYAALHNVGFPNMPVIVSETGWASHGDENEAGATLENARTYNKNLLKKLMKK 168
            F+AQVDAAYAAL   G+P MPVIVSETGWASHGDENEAGATL+NARTYN+NL KKLMKK
Sbjct: 239 MFEAQVDAAYAALEKAGYPKMPVIVSETGWASHGDENEAGATLKNARTYNRNLRKKLMKK 298

Query: 169 KGTPYQPKTVVKAYVFALFNENSKPRPTFERNFGLFKPDASIAYDI 208
           KGTP++PK VV+AY+FALFNENSKP PT ERNFGLFKPD SI+YDI
Sbjct: 299 KGTPFRPKMVVRAYIFALFNENSKPGPTSERNFGLFKPDGSISYDI 344

[Close]

BLAST of CmoCh01G001520 vs. ExPASy TrEMBL
Match: A0A1S3BY43 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103494697 PE=3 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 7.7e-67
Identity = 129/166 (77.71%), Postives = 140/166 (84.34%), Query Frame = 0


Query: 49  VVAFMNPLLQFFSLIASPFFINAYPFLAYNNDPQHIDINYALFNNNNRIFDPKT------ 108
           +V FM PLLQFFS I SPFFINAYPFLAY NDP+HIDINYALF  N  IFD KT      
Sbjct: 180 IVPFMKPLLQFFSFIGSPFFINAYPFLAYKNDPEHIDINYALFKKNKGIFDAKTNLHYDN 239

Query: 109 -FDAQVDAAYAALHNVGFPNMPVIVSETGWASHGDENEAGATLENARTYNKNLLKKLMKK 168
            F+AQVDAAYAAL   G+P MPVIVSETGWASHGDENEAGATL+NARTYN+NL KKLMKK
Sbjct: 240 MFEAQVDAAYAALEKAGYPKMPVIVSETGWASHGDENEAGATLKNARTYNRNLRKKLMKK 299

Query: 169 KGTPYQPKTVVKAYVFALFNENSKPRPTFERNFGLFKPDASIAYDI 208
           KGTP++PK VV+AY+FALFNENSKP PT ERNFGLFKPD SI+YDI
Sbjct: 300 KGTPFRPKMVVRAYIFALFNENSKPGPTSERNFGLFKPDGSISYDI 345

[Close]

BLAST of CmoCh01G001520 vs. ExPASy TrEMBL
Match: A0A6J1IWK6 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111479143 PE=3 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 8.5e-66
Identity = 128/166 (77.11%), Postives = 140/166 (84.34%), Query Frame = 0


Query: 49  VVAFMNPLLQFFSLIASPFFINAYPFLAYNNDPQHIDINYALFNNNNRIFDPKT------ 108
           +V FM PLL+FF+LI SPFFINAYPFLAY NDPQHIDI+YALF  N  IFD KT      
Sbjct: 177 IVPFMKPLLEFFNLIGSPFFINAYPFLAYKNDPQHIDIDYALFKKNKGIFDSKTNLHYDN 236

Query: 109 -FDAQVDAAYAALHNVGFPNMPVIVSETGWASHGDENEAGATLENARTYNKNLLKKLMKK 168
            FDAQVDAAY AL   G+P MPVIVSETGWASHGDENEAGATL+NARTYN+NL KKLMKK
Sbjct: 237 MFDAQVDAAYIALEKAGYPKMPVIVSETGWASHGDENEAGATLKNARTYNQNLRKKLMKK 296

Query: 169 KGTPYQPKTVVKAYVFALFNENSKPRPTFERNFGLFKPDASIAYDI 208
           KGTPY+PKTVV+AY+FALFNENSKP PT ERNFGLFK D SI+Y+I
Sbjct: 297 KGTPYRPKTVVRAYIFALFNENSKPGPTSERNFGLFKADGSISYNI 342

[Close]

BLAST of CmoCh01G001520 vs. NCBI nr
Match: XP_038895451.1 (glucan endo-1,3-beta-glucosidase 14 [Benincasa hispida])

HSP 1 Score: 267.7 bits (683), Expect = 8.4e-68
Identity = 132/166 (79.52%), Postives = 142/166 (85.54%), Query Frame = 0


Query: 49  VVAFMNPLLQFFSLIASPFFINAYPFLAYNNDPQHIDINYALFNNNNRIFDPKT------ 108
           +V FM PLLQFFSLI SPFFINAYPFLAY NDP+HIDINYALF  N  IFD KT      
Sbjct: 180 IVPFMKPLLQFFSLIGSPFFINAYPFLAYKNDPEHIDINYALFKKNKGIFDAKTNLHYDN 239

Query: 109 -FDAQVDAAYAALHNVGFPNMPVIVSETGWASHGDENEAGATLENARTYNKNLLKKLMKK 168
            F+AQVDAAYAAL   G+P MPVIVSETGWASHGDENEAGATL+NARTYN+NL KKLMKK
Sbjct: 240 MFEAQVDAAYAALEKAGYPKMPVIVSETGWASHGDENEAGATLKNARTYNRNLRKKLMKK 299

Query: 169 KGTPYQPKTVVKAYVFALFNENSKPRPTFERNFGLFKPDASIAYDI 208
           KGTP++PKTVV+AYVFALFNENSKP PT ERNFGLFKPD SI+YDI
Sbjct: 300 KGTPFRPKTVVRAYVFALFNENSKPGPTSERNFGLFKPDGSISYDI 345

[Close]

BLAST of CmoCh01G001520 vs. NCBI nr
Match: XP_011652976.1 (glucan endo-1,3-beta-glucosidase 14 isoform X2 [Cucumis sativus])

HSP 1 Score: 264.2 bits (674), Expect = 9.3e-67
Identity = 129/166 (77.71%), Postives = 141/166 (84.94%), Query Frame = 0


Query: 49  VVAFMNPLLQFFSLIASPFFINAYPFLAYNNDPQHIDINYALFNNNNRIFDPKT------ 108
           +V FM PLLQFFS I SPFFINAYPFLAY NDP+HIDINYALF  N  IFD KT      
Sbjct: 179 IVPFMKPLLQFFSFIGSPFFINAYPFLAYKNDPEHIDINYALFKKNKGIFDAKTNLHYDN 238

Query: 109 -FDAQVDAAYAALHNVGFPNMPVIVSETGWASHGDENEAGATLENARTYNKNLLKKLMKK 168
            F+AQVDAAYAAL  VG+P MPVIVSETGWASHGDENEAGAT++NARTYN+NL KKLMKK
Sbjct: 239 MFEAQVDAAYAALEKVGYPKMPVIVSETGWASHGDENEAGATMKNARTYNRNLRKKLMKK 298

Query: 169 KGTPYQPKTVVKAYVFALFNENSKPRPTFERNFGLFKPDASIAYDI 208
           KGTP++PK VV+AY+FALFNENSKP PT ERNFGLFKPD SI+YDI
Sbjct: 299 KGTPFRPKMVVRAYIFALFNENSKPGPTSERNFGLFKPDGSISYDI 344

[Close]

BLAST of CmoCh01G001520 vs. NCBI nr
Match: XP_011652975.1 (glucan endo-1,3-beta-glucosidase 14 isoform X1 [Cucumis sativus] >KGN52895.1 hypothetical protein Csa_014877 [Cucumis sativus])

HSP 1 Score: 264.2 bits (674), Expect = 9.3e-67
Identity = 129/166 (77.71%), Postives = 141/166 (84.94%), Query Frame = 0


Query: 49  VVAFMNPLLQFFSLIASPFFINAYPFLAYNNDPQHIDINYALFNNNNRIFDPKT------ 108
           +V FM PLLQFFS I SPFFINAYPFLAY NDP+HIDINYALF  N  IFD KT      
Sbjct: 180 IVPFMKPLLQFFSFIGSPFFINAYPFLAYKNDPEHIDINYALFKKNKGIFDAKTNLHYDN 239

Query: 109 -FDAQVDAAYAALHNVGFPNMPVIVSETGWASHGDENEAGATLENARTYNKNLLKKLMKK 168
            F+AQVDAAYAAL  VG+P MPVIVSETGWASHGDENEAGAT++NARTYN+NL KKLMKK
Sbjct: 240 MFEAQVDAAYAALEKVGYPKMPVIVSETGWASHGDENEAGATMKNARTYNRNLRKKLMKK 299

Query: 169 KGTPYQPKTVVKAYVFALFNENSKPRPTFERNFGLFKPDASIAYDI 208
           KGTP++PK VV+AY+FALFNENSKP PT ERNFGLFKPD SI+YDI
Sbjct: 300 KGTPFRPKMVVRAYIFALFNENSKPGPTSERNFGLFKPDGSISYDI 345

[Close]

BLAST of CmoCh01G001520 vs. NCBI nr
Match: XP_008454225.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 14 isoform X1 [Cucumis melo])

HSP 1 Score: 263.5 bits (672), Expect = 1.6e-66
Identity = 129/166 (77.71%), Postives = 140/166 (84.34%), Query Frame = 0


Query: 49  VVAFMNPLLQFFSLIASPFFINAYPFLAYNNDPQHIDINYALFNNNNRIFDPKT------ 108
           +V FM PLLQFFS I SPFFINAYPFLAY NDP+HIDINYALF  N  IFD KT      
Sbjct: 180 IVPFMKPLLQFFSFIGSPFFINAYPFLAYKNDPEHIDINYALFKKNKGIFDAKTNLHYDN 239

Query: 109 -FDAQVDAAYAALHNVGFPNMPVIVSETGWASHGDENEAGATLENARTYNKNLLKKLMKK 168
            F+AQVDAAYAAL   G+P MPVIVSETGWASHGDENEAGATL+NARTYN+NL KKLMKK
Sbjct: 240 MFEAQVDAAYAALEKAGYPKMPVIVSETGWASHGDENEAGATLKNARTYNRNLRKKLMKK 299

Query: 169 KGTPYQPKTVVKAYVFALFNENSKPRPTFERNFGLFKPDASIAYDI 208
           KGTP++PK VV+AY+FALFNENSKP PT ERNFGLFKPD SI+YDI
Sbjct: 300 KGTPFRPKMVVRAYIFALFNENSKPGPTSERNFGLFKPDGSISYDI 345

[Close]

BLAST of CmoCh01G001520 vs. NCBI nr
Match: KAA0044445.1 (glucan endo-1,3-beta-glucosidase 14 isoform X1 [Cucumis melo var. makuwa] >TYK29572.1 glucan endo-1,3-beta-glucosidase 14 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 263.5 bits (672), Expect = 1.6e-66
Identity = 129/166 (77.71%), Postives = 140/166 (84.34%), Query Frame = 0


Query: 49  VVAFMNPLLQFFSLIASPFFINAYPFLAYNNDPQHIDINYALFNNNNRIFDPKT------ 108
           +V FM PLLQFFS I SPFFINAYPFLAY NDP+HIDINYALF  N  IFD KT      
Sbjct: 538 IVPFMKPLLQFFSFIGSPFFINAYPFLAYKNDPEHIDINYALFKKNKGIFDAKTNLHYDN 597

Query: 109 -FDAQVDAAYAALHNVGFPNMPVIVSETGWASHGDENEAGATLENARTYNKNLLKKLMKK 168
            F+AQVDAAYAAL   G+P MPVIVSETGWASHGDENEAGATL+NARTYN+NL KKLMKK
Sbjct: 598 MFEAQVDAAYAALEKAGYPKMPVIVSETGWASHGDENEAGATLKNARTYNRNLRKKLMKK 657

Query: 169 KGTPYQPKTVVKAYVFALFNENSKPRPTFERNFGLFKPDASIAYDI 208
           KGTP++PK VV+AY+FALFNENSKP PT ERNFGLFKPD SI+YDI
Sbjct: 658 KGTPFRPKMVVRAYIFALFNENSKPGPTSERNFGLFKPDGSISYDI 703

[Close]

BLAST of CmoCh01G001520 vs. TAIR 10
Match: AT3G15800.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 225.3 bits (573), Expect = 4.5e-59
Identity = 113/166 (68.07%), Postives = 129/166 (77.71%), Query Frame = 0


Query: 49  VVAFMNPLLQFFSLIASPFFINAYPFLAYNNDPQHIDINYALFNNNNRIFDPKT------ 108
           V  FM PLL FF  I SPF+INAYPFLAY +DP  IDINYALF +N  I DPKT      
Sbjct: 196 VAPFMKPLLAFFWQIQSPFYINAYPFLAYKSDPITIDINYALFEHNKGILDPKTKLHYDN 255

Query: 109 -FDAQVDAAYAALHNVGFPNMPVIVSETGWASHGDENEAGATLENARTYNKNLLKKLMKK 168
            FDA VDA+YAAL   G+  +PVIVSETGWAS GD +E GA+++NARTYN+NL K+L K+
Sbjct: 256 MFDAMVDASYAALEKAGYTKVPVIVSETGWASKGDADEPGASVKNARTYNRNLRKRLQKR 315

Query: 169 KGTPYQPKTVVKAYVFALFNENSKPRPTFERNFGLFKPDASIAYDI 208
           KGTPY+P  VV+AYVFALFNENSKP PT ERNFGLFKPD +IAYDI
Sbjct: 316 KGTPYRPDMVVRAYVFALFNENSKPGPTSERNFGLFKPDGTIAYDI 361

[Close]

BLAST of CmoCh01G001520 vs. TAIR 10
Match: AT2G26600.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 209.5 bits (532), Expect = 2.5e-54
Identity = 105/166 (63.25%), Postives = 124/166 (74.70%), Query Frame = 0


Query: 49  VVAFMNPLLQFFSLIASPFFINAYPFLAYNNDPQHIDINYALFNNNNRIFDPKT------ 108
           VV FM PLL+FF  I SPF +NAYPFLAY  +P+ IDINYALF     I+DPKT      
Sbjct: 186 VVQFMKPLLEFFQQIGSPFCLNAYPFLAYTYNPKEIDINYALFKPTEGIYDPKTDLHYDN 245

Query: 109 -FDAQVDAAYAALHNVGFPNMPVIVSETGWASHGDENEAGATLENARTYNKNLLKKLMKK 168
             DAQ+DAAY AL + GF  M V+++ETGWAS GD +E  AT ENARTYN NL K+L KK
Sbjct: 246 MLDAQIDAAYMALQDAGFKKMEVMITETGWASKGDSDEPAATPENARTYNYNLRKRLAKK 305

Query: 169 KGTPYQPKTVVKAYVFALFNENSKPRPTFERNFGLFKPDASIAYDI 208
           KGTP +PKTV+KAY+FALFNENSKP  + E +FGLFKPD +I+YDI
Sbjct: 306 KGTPLRPKTVLKAYIFALFNENSKPGKSSETHFGLFKPDGTISYDI 351

[Close]

BLAST of CmoCh01G001520 vs. TAIR 10
Match: AT2G26600.2 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 209.5 bits (532), Expect = 2.5e-54
Identity = 105/166 (63.25%), Postives = 124/166 (74.70%), Query Frame = 0


Query: 49  VVAFMNPLLQFFSLIASPFFINAYPFLAYNNDPQHIDINYALFNNNNRIFDPKT------ 108
           VV FM PLL+FF  I SPF +NAYPFLAY  +P+ IDINYALF     I+DPKT      
Sbjct: 92  VVQFMKPLLEFFQQIGSPFCLNAYPFLAYTYNPKEIDINYALFKPTEGIYDPKTDLHYDN 151

Query: 109 -FDAQVDAAYAALHNVGFPNMPVIVSETGWASHGDENEAGATLENARTYNKNLLKKLMKK 168
             DAQ+DAAY AL + GF  M V+++ETGWAS GD +E  AT ENARTYN NL K+L KK
Sbjct: 152 MLDAQIDAAYMALQDAGFKKMEVMITETGWASKGDSDEPAATPENARTYNYNLRKRLAKK 211

Query: 169 KGTPYQPKTVVKAYVFALFNENSKPRPTFERNFGLFKPDASIAYDI 208
           KGTP +PKTV+KAY+FALFNENSKP  + E +FGLFKPD +I+YDI
Sbjct: 212 KGTPLRPKTVLKAYIFALFNENSKPGKSSETHFGLFKPDGTISYDI 257

[Close]

BLAST of CmoCh01G001520 vs. TAIR 10
Match: AT2G27500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 162.9 bits (411), Expect = 2.7e-40
Identity = 82/165 (49.70%), Postives = 106/165 (64.24%), Query Frame = 0


Query: 50  VAFMNPLLQFFSLIASPFFINAYPFLAYNNDPQHIDINYALFNNNNRIFDPKT---FD-- 109
           + ++ PLL F S I SPF INAYPF AY + P+ + + Y LF  N  + DP T   +D  
Sbjct: 182 IQYLQPLLDFHSQIESPFLINAYPFFAYKDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNM 241

Query: 110 --AQVDAAYAALHNVGFPNMPVIVSETGWASHGDENEAGATLENARTYNKNLLKKLMKKK 169
             AQVDA Y+A+  +G  ++ V +SETGW S GDENE GA+ ENA  YN NLLK + ++K
Sbjct: 242 LFAQVDALYSAIKTLGHTDIEVRISETGWPSKGDENEIGASPENAALYNGNLLKLIQQRK 301

Query: 170 GTPYQPKTVVKAYVFALFNENSKPRPTFERNFGLFKPDASIAYDI 208
           GTP +    +  YVFALFNEN KP P  ERN+GLF PD    Y++
Sbjct: 302 GTPAKQSVPIDVYVFALFNENLKPGPVSERNYGLFYPDGKPVYNV 346

[Close]

BLAST of CmoCh01G001520 vs. TAIR 10
Match: AT2G27500.2 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 162.9 bits (411), Expect = 2.7e-40
Identity = 82/165 (49.70%), Postives = 106/165 (64.24%), Query Frame = 0


Query: 50  VAFMNPLLQFFSLIASPFFINAYPFLAYNNDPQHIDINYALFNNNNRIFDPKT---FD-- 109
           + ++ PLL F S I SPF INAYPF AY + P+ + + Y LF  N  + DP T   +D  
Sbjct: 182 IQYLQPLLDFHSQIESPFLINAYPFFAYKDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNM 241

Query: 110 --AQVDAAYAALHNVGFPNMPVIVSETGWASHGDENEAGATLENARTYNKNLLKKLMKKK 169
             AQVDA Y+A+  +G  ++ V +SETGW S GDENE GA+ ENA  YN NLLK + ++K
Sbjct: 242 LFAQVDALYSAIKTLGHTDIEVRISETGWPSKGDENEIGASPENAALYNGNLLKLIQQRK 301

Query: 170 GTPYQPKTVVKAYVFALFNENSKPRPTFERNFGLFKPDASIAYDI 208
           GTP +    +  YVFALFNEN KP P  ERN+GLF PD    Y++
Sbjct: 302 GTPAKQSVPIDVYVFALFNENLKPGPVSERNYGLFYPDGKPVYNV 346

The following BLAST results are available for this feature:

BLAST of CmoCh01G001520 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZQG9	3.8e-39	49.70	Glucan endo-1,3-beta-glucosidase 14 OS=Arabidopsis thaliana OX=3702 GN=At2g27500...	[more]
Q8L868	1.5e-38	50.61	Glucan endo-1,3-beta-glucosidase 11 OS=Arabidopsis thaliana OX=3702 GN=At1g32860...	[more]
Q9FHX5	2.3e-36	48.48	Glucan endo-1,3-beta-glucosidase 10 OS=Arabidopsis thaliana OX=3702 GN=At5g42100...	[more]
Q9M069	2.3e-36	49.06	Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ...	[more]
Q9FJU9	1.7e-31	40.24	Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590...	[more]

BLAST of CmoCh01G001520 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KTE7	4.5e-67	77.71	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_4G005530 PE=3...	[more]
A0A5A7TSV0	7.7e-67	77.71	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3BYW7	7.7e-67	77.71	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103494697 PE=3 SV...	[more]
A0A1S3BY43	7.7e-67	77.71	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103494697 PE=3 SV...	[more]
A0A6J1IWK6	8.5e-66	77.11	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111479143 PE=...	[more]

BLAST of CmoCh01G001520 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038895451.1	8.4e-68	79.52	glucan endo-1,3-beta-glucosidase 14 [Benincasa hispida]	[more]
XP_011652976.1	9.3e-67	77.71	glucan endo-1,3-beta-glucosidase 14 isoform X2 [Cucumis sativus]	[more]
XP_011652975.1	9.3e-67	77.71	glucan endo-1,3-beta-glucosidase 14 isoform X1 [Cucumis sativus] >KGN52895.1 hyp...	[more]
XP_008454225.1	1.6e-66	77.71	PREDICTED: glucan endo-1,3-beta-glucosidase 14 isoform X1 [Cucumis melo]	[more]
KAA0044445.1	1.6e-66	77.71	glucan endo-1,3-beta-glucosidase 14 isoform X1 [Cucumis melo var. makuwa] >TYK29...	[more]

BLAST of CmoCh01G001520 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G15800.1	4.5e-59	68.07	Glycosyl hydrolase superfamily protein	[more]
AT2G26600.1	2.5e-54	63.25	Glycosyl hydrolase superfamily protein	[more]
AT2G26600.2	2.5e-54	63.25	Glycosyl hydrolase superfamily protein	[more]
AT2G27500.1	2.7e-40	49.70	Glycosyl hydrolase superfamily protein	[more]
AT2G27500.2	2.7e-40	49.70	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 50..207 e-value: 2.9E-42 score: 145.4
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 16..209 e-value: 4.8E-56 score: 192.0
None	No IPR available	PANTHER	PTHR32227:SF357	GLYCOSYL HYDROLASE SUPERFAMILY PROTEIN	coord: 49..207
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 49..207
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 48..207

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh01G001520.1	CmoCh01G001520.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0046658	anchored component of plasma membrane
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds