CmoCh01G001060 (gene) Cucurbita moschata (Rifu) v1

BLAST of CmoCh01G001060 vs. ExPASy Swiss-Prot
Match: Q9C5H4 (Protein MODIFIED TRANSPORT TO THE VACUOLE 1 OS=Arabidopsis thaliana OX=3702 GN=MTV1 PE=1 SV=1)

HSP 1 Score: 627.5 bits (1617), Expect = 1.8e-178
Identity = 375/692 (54.19%), Postives = 476/692 (68.79%), Query Frame = 0

Query: 1   MIDAATSDEDKVTPVYKLEEISEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK 60
           MIDA TSDEDKV PVYKLEEI ++LRSSHVSIVKEFSEFILKRL++KSPIVKQKALRLIK
Sbjct: 16  MIDAVTSDEDKVAPVYKLEEICDLLRSSHVSIVKEFSEFILKRLDNKSPIVKQKALRLIK 75

Query: 61  YGIGKSGVEFRREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRDTAQDAISAIFAEED- 120
           Y +GKSG EFRREMQR+SVAVR L HYKG PDPLKGDALNKAVR+TA + ISAIF+EE+ 
Sbjct: 76  YAVGKSGSEFRREMQRNSVAVRNLFHYKGHPDPLKGDALNKAVRETAHETISAIFSEENG 135

Query: 121 NRPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQGLSNLAQGHSSRK-- 180
            +PA  E++N RI+GFGN+N++ P+ D KSFLSEVVG+GSASIKQG+SN AQGH  +K  
Sbjct: 136 TKPAAPESINRRIEGFGNTNFQVPSNDNKSFLSEVVGIGSASIKQGISNFAQGHLPKKNE 195

Query: 181 NGTSGHRGPNLQRSLTTEMEYDNRYEPVEYGRE-TLGTSKSMISGPWNPDSWANKVEATN 240
           NG+S +RGPNL RSLT E E  +RY+PV+ G++   GTSK+   G     SW +     +
Sbjct: 196 NGSSSYRGPNLHRSLTMENENFSRYDPVKLGKDGNYGTSKNTTGG-----SWGHASGEAS 255

Query: 241 GNLSSGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAMALSSALESKLK 300
            + +S   E KTREE+LLETI T+GGVR+QPTRDA+  F++EAAK+DA+ALS AL+ KL 
Sbjct: 256 ESSASVRVESKTREEKLLETIVTSGGVRLQPTRDALHVFILEAAKMDAVALSIALDGKLH 315

Query: 301 SPSWQVRFKALCILESIVRKNDDDHFSIVASYFSENQDAVIGCSESPQASLREKASKVMP 360
           SP WQVR KALC+LE+I+RK +D++FSIV +YFSEN DA+  C+ESPQ+SLREKA+KV+ 
Sbjct: 316 SPMWQVRMKALCVLEAILRKKEDENFSIVHTYFSENLDAIQRCAESPQSSLREKANKVLS 375

Query: 361 LLDGGKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDA----GVLEAENLSKT-----PLV 420
           LL+GG+    M+ S+ +      + + +PDLIDT D+      L A +   T     PL+
Sbjct: 376 LLNGGQSSGLMSSSDNTVKR--EAAVDLPDLIDTGDSDDTLNNLNAIDTGSTVATAGPLM 435

Query: 421 -DVLFGDGVNTVASTSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNSQVSNENKRP 480
            D  FGD  +   S+SE K DDDPF+DVSF   +  E+ DDLFSGM V         K  
Sbjct: 436 DDDWFGDSSDIGLSSSEKKTDDDPFADVSFHPNEEKESADDLFSGMTVG-------EKSA 495

Query: 481 ASEQKNEHGVFDVLGSSSEAAVQEHTRKDVNDLMSGLSIHEDGLKSNDIGDSKDSLSESL 540
           A    +   +FD+ GS+++   +    K++NDLM   SI E+   SN  G S  +L + L
Sbjct: 496 AVGGNHVPDLFDMFGSTAKLEAEPKDAKNINDLMGSFSIDEN--NSNQKGSSSSTLPQDL 555

Query: 541 YSVSGQPNHQYQTKDSSVNGIYSSPMVGTNMNAAFFPGMT--YLPSGMMFNPAFSSRPMG 600
           +++    +H  Q  ++ V GI  S   G   N     G+     P GMM NPAF+S+P+ 
Sbjct: 556 FAMPSTTSH--QAPENPVGGILGSQNPGFIQNTMLPGGVMPFNFPQGMMMNPAFASQPLN 615

Query: 601 YAPTGNFFA-QQNLVSAMSNYQQFGNPHLQSSGG--SAG-NGGYSSPLPDIFQPNLATQP 660
           YA   +  A QQ  +  MSN+QQFGN + Q SG   S G +GG  S LPDIFQPN   Q 
Sbjct: 616 YAAMASLLAQQQQYLGNMSNFQQFGNLNAQGSGNVLSMGTSGGNQSALPDIFQPNFGNQA 675

Query: 661 SSSVMNSSKKEDTRAFDFISEHIAAARDPKRV 673
            +S MN SKKEDTRAFDFIS+H+ +ARD KRV
Sbjct: 676 PTSTMNGSKKEDTRAFDFISDHLTSARDTKRV 689

BLAST of CmoCh01G001060 vs. ExPASy Swiss-Prot
Match: G3V8Y7 (AP-4 complex accessory subunit Tepsin OS=Rattus norvegicus OX=10116 GN=Tepsin PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 3.9e-08
Identity = 47/147 (31.97%), Postives = 75/147 (51.02%), Query Frame = 0

Query: 6   TSDEDKVTPVYKLEEISEVLRSSHVSIVKE--FSEFILKRLEHKSPIVKQKALRLIKYGI 65
           TSD+D   P Y  EEI+++   SH S+       E++L RL+  S  VK K L+++ Y  
Sbjct: 24  TSDDDIPCPGYLFEEIAKI---SHESLGSSQCLLEYLLNRLDSSSGHVKLKVLKILLYLC 83

Query: 66  GKSGVEFRREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRDTAQDAISAIFAEEDNRPA 125
                 F   ++R+S  +++   + G PDPL G++L + VR  AQD  S +F++   +P 
Sbjct: 84  SHGSSSFMLILRRNSALIQEATAFAGPPDPLHGNSLYQKVRAAAQDLGSTLFSDALPQP- 143

Query: 126 PSE--------------NLNSRIQGFG 137
           PS+                +S +QGFG
Sbjct: 144 PSQPPQILPPAGMGAQARPHSALQGFG 166

BLAST of CmoCh01G001060 vs. ExPASy Swiss-Prot
Match: Q3U3N6 (AP-4 complex accessory subunit Tepsin OS=Mus musculus OX=10090 GN=Tepsin PE=2 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 4.7e-06
Identity = 89/359 (24.79%), Postives = 146/359 (40.67%), Query Frame = 0

Query: 6   TSDEDKVTPVYKLEEISEVLRSSHVSIVKE--FSEFILKRLEHKSPIVKQKALRLIKYGI 65
           TSD+    P Y  EEI+++   SH S+       E++L RL+  S  VK K L+++ Y  
Sbjct: 24  TSDDSIPCPGYLFEEIAKI---SHESLGSSQCLLEYLLNRLDSSSGHVKLKVLKILLYLC 83

Query: 66  GKSGVEFRREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRDTAQDAISAIFAEEDNRP- 125
           G     F   ++R+S  +++   + G PDPL G++L + VR  AQD  S +F++   +P 
Sbjct: 84  GHGSSSFLLILRRNSALIQEATAFSGPPDPLHGNSLYQKVRAAAQDLGSTLFSDAVPQPP 143

Query: 126 ------APSENLN------SRIQGFG----NSNYEPPAEDKKSFLSEVVGLGSASIKQGL 185
                  P   +       S +QGFG    +S      E   S +     + + +++ G 
Sbjct: 144 SQPPQIPPPAGMGAQARPLSALQGFGYTKESSRTGSAGETFLSTIQRAAEVVANAVRPGP 203

Query: 186 SN------LAQGHSSRKNGT--SGHRGPNLQRSLTTEM--EYDNRYEPVEYGRETLGTSK 245
            N      L  G S +   T  + H  PN    L   +      R++P + G    G   
Sbjct: 204 DNPCTKGPLPYGDSYQPAVTPSASHTHPNPGNLLPGAILGARAVRHQPGQAG----GGWD 263

Query: 246 SMISGP--WNPDSWANKVEATNGNLSSGSSERK---------------------TREERL 305
            + S P   N    +N   A++    SGS                          +E  L
Sbjct: 264 ELDSSPSSQNSSCTSNLSRASDSGSRSGSDSHSGTSREPGDLAERAEATPPNDCQQELNL 323

Query: 306 LETIATAGGVRIQPTRDAIQAFLVEAAKLDAMALSSALESKLKSPSWQVRFKALCILES 313
           + T+    G R+  +R+  Q F+ E   L+  A+   L  +L   S   + +ALC + S
Sbjct: 324 VRTVTQ--GPRVFLSREETQHFIKECGLLNCEAVLELLLRQLVGTSECEQMRALCAIAS 373

BLAST of CmoCh01G001060 vs. ExPASy TrEMBL
Match: A0A6J1GBM7 (VHS domain-containing protein At3g16270-like OS=Cucurbita moschata OX=3662 GN=LOC111452495 PE=4 SV=1)

HSP 1 Score: 1291.9 bits (3342), Expect = 0.0e+00
Identity = 673/673 (100.00%), Postives = 673/673 (100.00%), Query Frame = 0

Query: 1   MIDAATSDEDKVTPVYKLEEISEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK 60
           MIDAATSDEDKVTPVYKLEEISEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK
Sbjct: 16  MIDAATSDEDKVTPVYKLEEISEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK 75

Query: 61  YGIGKSGVEFRREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRDTAQDAISAIFAEEDN 120
           YGIGKSGVEFRREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRDTAQDAISAIFAEEDN
Sbjct: 76  YGIGKSGVEFRREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRDTAQDAISAIFAEEDN 135

Query: 121 RPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQGLSNLAQGHSSRKNGT 180
           RPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQGLSNLAQGHSSRKNGT
Sbjct: 136 RPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQGLSNLAQGHSSRKNGT 195

Query: 181 SGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSMISGPWNPDSWANKVEATNGNLS 240
           SGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSMISGPWNPDSWANKVEATNGNLS
Sbjct: 196 SGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSMISGPWNPDSWANKVEATNGNLS 255

Query: 241 SGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAMALSSALESKLKSPSW 300
           SGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAMALSSALESKLKSPSW
Sbjct: 256 SGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAMALSSALESKLKSPSW 315

Query: 301 QVRFKALCILESIVRKNDDDHFSIVASYFSENQDAVIGCSESPQASLREKASKVMPLLDG 360
           QVRFKALCILESIVRKNDDDHFSIVASYFSENQDAVIGCSESPQASLREKASKVMPLLDG
Sbjct: 316 QVRFKALCILESIVRKNDDDHFSIVASYFSENQDAVIGCSESPQASLREKASKVMPLLDG 375

Query: 361 GKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAGVLEAENLSKTPLVDVLFGDGVNTVAS 420
           GKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAGVLEAENLSKTPLVDVLFGDGVNTVAS
Sbjct: 376 GKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAGVLEAENLSKTPLVDVLFGDGVNTVAS 435

Query: 421 TSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNSQVSNENKRPASEQKNEHGVFDVL 480
           TSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNSQVSNENKRPASEQKNEHGVFDVL
Sbjct: 436 TSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNSQVSNENKRPASEQKNEHGVFDVL 495

Query: 481 GSSSEAAVQEHTRKDVNDLMSGLSIHEDGLKSNDIGDSKDSLSESLYSVSGQPNHQYQTK 540
           GSSSEAAVQEHTRKDVNDLMSGLSIHEDGLKSNDIGDSKDSLSESLYSVSGQPNHQYQTK
Sbjct: 496 GSSSEAAVQEHTRKDVNDLMSGLSIHEDGLKSNDIGDSKDSLSESLYSVSGQPNHQYQTK 555

Query: 541 DSSVNGIYSSPMVGTNMNAAFFPGMTYLPSGMMFNPAFSSRPMGYAPTGNFFAQQNLVSA 600
           DSSVNGIYSSPMVGTNMNAAFFPGMTYLPSGMMFNPAFSSRPMGYAPTGNFFAQQNLVSA
Sbjct: 556 DSSVNGIYSSPMVGTNMNAAFFPGMTYLPSGMMFNPAFSSRPMGYAPTGNFFAQQNLVSA 615

Query: 601 MSNYQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVMNSSKKEDTRAFDFIS 660
           MSNYQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVMNSSKKEDTRAFDFIS
Sbjct: 616 MSNYQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVMNSSKKEDTRAFDFIS 675

Query: 661 EHIAAARDPKRVV 674
           EHIAAARDPKRVV
Sbjct: 676 EHIAAARDPKRVV 688

BLAST of CmoCh01G001060 vs. ExPASy TrEMBL
Match: A0A6J1K668 (VHS domain-containing protein At3g16270-like OS=Cucurbita maxima OX=3661 GN=LOC111492683 PE=4 SV=1)

HSP 1 Score: 1248.0 bits (3228), Expect = 0.0e+00
Identity = 651/673 (96.73%), Postives = 659/673 (97.92%), Query Frame = 0

Query: 1   MIDAATSDEDKVTPVYKLEEISEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK 60
           MIDAATSDEDKVTPVYKLEEI EVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK
Sbjct: 16  MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK 75

Query: 61  YGIGKSGVEFRREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRDTAQDAISAIFAEEDN 120
           YG+GKSGVEFRREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRDTAQDAISAIFAEEDN
Sbjct: 76  YGVGKSGVEFRREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRDTAQDAISAIFAEEDN 135

Query: 121 RPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQGLSNLAQGHSSRKNGT 180
           RPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQGLSNLAQGHSSRKNGT
Sbjct: 136 RPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQGLSNLAQGHSSRKNGT 195

Query: 181 SGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSMISGPWNPDSWANKVEATNGNLS 240
           SGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKS ISGPWNPDSWANKVEATNGNLS
Sbjct: 196 SGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSTISGPWNPDSWANKVEATNGNLS 255

Query: 241 SGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAMALSSALESKLKSPSW 300
           SGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAMALSSALESKLKSPSW
Sbjct: 256 SGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAMALSSALESKLKSPSW 315

Query: 301 QVRFKALCILESIVRKNDDDHFSIVASYFSENQDAVIGCSESPQASLREKASKVMPLLDG 360
           QVRFKALC+LESIVRKNDDDHFSIVASYFSENQDAVIGCSESPQASLREKASKVMPLLDG
Sbjct: 316 QVRFKALCVLESIVRKNDDDHFSIVASYFSENQDAVIGCSESPQASLREKASKVMPLLDG 375

Query: 361 GKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAGVLEAENLSKTPLVDVLFGDGVNTVAS 420
           GKGVPSMN SEKS PNNPSSTIQMPDLIDTSDAGVLE ENLSKTPLVD LFGDGVNT+ S
Sbjct: 376 GKGVPSMNASEKSLPNNPSSTIQMPDLIDTSDAGVLEVENLSKTPLVDDLFGDGVNTITS 435

Query: 421 TSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNSQVSNENKRPASEQKNEHGVFDVL 480
           TSELKNDDDPFSDVSFQTTD+GENPDDLFSGM VDNSQVSNE+KRPASEQKNEHGVFDV 
Sbjct: 436 TSELKNDDDPFSDVSFQTTDDGENPDDLFSGMIVDNSQVSNESKRPASEQKNEHGVFDVF 495

Query: 481 GSSSEAAVQEHTRKDVNDLMSGLSIHEDGLKSNDIGDSKDSLSESLYSVSGQPNHQYQTK 540
           GSSSEAAVQEHTRKDVN+LMSGLSIHEDGLKSNDIGDSKDSLSESLYSVS QPNHQYQTK
Sbjct: 496 GSSSEAAVQEHTRKDVNELMSGLSIHEDGLKSNDIGDSKDSLSESLYSVSRQPNHQYQTK 555

Query: 541 DSSVNGIYSSPMVGTNMNAAFFPGMTYLPSGMMFNPAFSSRPMGYAPTGNFFAQQNLVSA 600
           DSSVNGIYSSPMVGTNMNAA FPG TYLPSGMMFNPAFSS+PMGYAPTGNFFAQQNL SA
Sbjct: 556 DSSVNGIYSSPMVGTNMNAALFPGRTYLPSGMMFNPAFSSQPMGYAPTGNFFAQQNLPSA 615

Query: 601 MSNYQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVMNSSKKEDTRAFDFIS 660
           MSN QQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVMNSSKKEDTRAFDFIS
Sbjct: 616 MSNSQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVMNSSKKEDTRAFDFIS 675

Query: 661 EHIAAARDPKRVV 674
           EHIAAARDPK+VV
Sbjct: 676 EHIAAARDPKQVV 688

BLAST of CmoCh01G001060 vs. ExPASy TrEMBL
Match: A0A1S3BH26 (VHS domain-containing protein At3g16270 OS=Cucumis melo OX=3656 GN=LOC103489958 PE=4 SV=1)

HSP 1 Score: 1091.6 bits (2822), Expect = 0.0e+00
Identity = 576/681 (84.58%), Postives = 613/681 (90.01%), Query Frame = 0

Query: 1   MIDAATSDEDKVTPVYKLEEISEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK 60
           MIDAATSDEDKVTPVYKLEEI EVLRSSHVSIVKEFSEFILKRLEHKSP+VKQKALRLIK
Sbjct: 16  MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSPVVKQKALRLIK 75

Query: 61  YGIGKSGVEFRREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRDTAQDAISAIFAEEDN 120
           Y +GKSGVEFRREMQR+SVAVRQL HYKGQPDPLKGDALNKAVRDTA +AIS+IFAEEDN
Sbjct: 76  YAVGKSGVEFRREMQRNSVAVRQLFHYKGQPDPLKGDALNKAVRDTAHEAISSIFAEEDN 135

Query: 121 RPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQGLSNLAQGHSSRKNGT 180
           +PAPSENLN RIQGFGNSNYEPP+EDKKSFLSEVVGLGSASIKQGLSN AQGHSSRKNGT
Sbjct: 136 KPAPSENLNRRIQGFGNSNYEPPSEDKKSFLSEVVGLGSASIKQGLSNFAQGHSSRKNGT 195

Query: 181 SGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSMISGPWNPDSWANKVEATNGNLS 240
           S HRG NLQRSLTTEMEYDNRYEPVEYGRETLGT++S  SG WN DS       +NG+ S
Sbjct: 196 SSHRGINLQRSLTTEMEYDNRYEPVEYGRETLGTARSTTSGTWNQDS-----RVSNGSPS 255

Query: 241 SGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAMALSSALESKLKSPSW 300
           SGSSE KTRE+RLL+TIATAGGVR+QPTRD+IQAFLVEA KLDA+ALS+ALE+KLKSPSW
Sbjct: 256 SGSSESKTREDRLLDTIATAGGVRLQPTRDSIQAFLVEAVKLDALALSNALETKLKSPSW 315

Query: 301 QVRFKALCILESIVRKNDDDHFSIVASYFSENQDAVIGCSESPQASLREKASKVMPLLDG 360
           QVRFKALCILESIVR+NDDDHFSIV SYFSENQ+AVIGCSESPQASLREKASKVMPLLDG
Sbjct: 316 QVRFKALCILESIVRRNDDDHFSIVTSYFSENQEAVIGCSESPQASLREKASKVMPLLDG 375

Query: 361 GKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAG-------VLEAENLSKTPLVDVLFGD 420
           GKGVPSMN SEKS P+N SSTIQMPDLIDTSDAG        +E ENLS TPLVD LFGD
Sbjct: 376 GKGVPSMNVSEKSLPSNTSSTIQMPDLIDTSDAGDYSGTNKSVEVENLSSTPLVDDLFGD 435

Query: 421 GVNTVASTSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNSQVSNENKRPASEQKNE 480
           G+NTV STSELKNDDDPFSDVSF T +  ENPDDLFSGMN DN+QVSNENK+PA EQKNE
Sbjct: 436 GLNTVTSTSELKNDDDPFSDVSFHTIETRENPDDLFSGMNFDNNQVSNENKKPALEQKNE 495

Query: 481 HGVFDVLGSSSEAAVQEHTRKDVNDLMSGLSIHEDGLKSNDIGDSKDSLSESLYSVSGQP 540
            GVFD+ GSSSE AVQEH RKDVNDLMSGLSIHED LKS D GDSKDSLSESL+S SGQP
Sbjct: 496 PGVFDIFGSSSEPAVQEHARKDVNDLMSGLSIHEDTLKSKDKGDSKDSLSESLFSASGQP 555

Query: 541 NHQYQTKDSSVNGIYSSPMVGTNMNAAFFPGMTYLPSGMMFNPAFSSRPMGYAPTGNFFA 600
           NHQ      S+NGIYSSPM GTNMNAAFFPGMTYLPSGMMFNPAFSS+PM YA +GNFF 
Sbjct: 556 NHQNPVSQDSLNGIYSSPMAGTNMNAAFFPGMTYLPSGMMFNPAFSSQPMAYAASGNFFT 615

Query: 601 QQNLVSAMSNYQQFGNPHLQS-SGGSAGNGGYSSPLPDIFQPNLATQPSSSVMNSSKKED 660
           QQ L+SAMSNYQQFGNP+LQS SGG  G+GGYSSPLPDIFQPNLA Q S+SVMNSSKKED
Sbjct: 616 QQQLLSAMSNYQQFGNPNLQSNSGGGVGSGGYSSPLPDIFQPNLAAQSSTSVMNSSKKED 675

Query: 661 TRAFDFISEHIAAARDPKRVV 674
           TRAFDFIS+H+AAARDPKRVV
Sbjct: 676 TRAFDFISDHVAAARDPKRVV 691

BLAST of CmoCh01G001060 vs. ExPASy TrEMBL
Match: A0A6J1IW71 (VHS domain-containing protein At3g16270-like OS=Cucurbita maxima OX=3661 GN=LOC111479156 PE=4 SV=1)

HSP 1 Score: 1078.2 bits (2787), Expect = 0.0e+00
Identity = 572/680 (84.12%), Postives = 604/680 (88.82%), Query Frame = 0

Query: 1   MIDAATSDEDKVTPVYKLEEISEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK 60
           MIDAATSDEDKVTPVYKLEEI EVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK
Sbjct: 16  MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK 75

Query: 61  YGIGKSGVEFRREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRDTAQDAISAIFAEEDN 120
           Y +GKSGVEFRREMQRHSVAVRQL HYKGQPDPLKGDALNKAVR+TA DAISAIFAEEDN
Sbjct: 76  YAVGKSGVEFRREMQRHSVAVRQLFHYKGQPDPLKGDALNKAVRETAHDAISAIFAEEDN 135

Query: 121 RPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQGLSNLAQGHSSRKNGT 180
           +PAPSENLN RIQGFGNSNYEPP EDKKSFLSEVVGLGSASIKQGLSN AQGHSSRKNGT
Sbjct: 136 KPAPSENLNRRIQGFGNSNYEPPPEDKKSFLSEVVGLGSASIKQGLSNFAQGHSSRKNGT 195

Query: 181 SGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSMISGPWNPDSWANKVEATNGNLS 240
           S  RGPNLQRSLTTE+EYDNRYEPVEYGRETLGTSKS ISG WN DS       +NGN S
Sbjct: 196 SSPRGPNLQRSLTTEIEYDNRYEPVEYGRETLGTSKSTISGTWNQDS-----RVSNGNSS 255

Query: 241 SGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAMALSSALESKLKSPSW 300
           SGSS  KTREERLLETIATAGGVR+QPTRDAIQAFLVEAA LDA+ALS+ALE+KLKSPSW
Sbjct: 256 SGSSVSKTREERLLETIATAGGVRLQPTRDAIQAFLVEAAMLDALALSNALETKLKSPSW 315

Query: 301 QVRFKALCILESIVRKNDDDHFSIVASYFSENQDAVIGCSESPQASLREKASKVMPLLDG 360
           QVRFKALCILESIVR++ D+HFSIV SYFSENQDAVIGCSESPQASLR+KASKVMPLLDG
Sbjct: 316 QVRFKALCILESIVRRSGDEHFSIVTSYFSENQDAVIGCSESPQASLRDKASKVMPLLDG 375

Query: 361 GKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAG-------VLEAENLSKTPLVDVLFGD 420
           GKGVP MNDSEKS P+N SSTIQMPDL+DTSDAG        LE ENLS  PLVD LFG 
Sbjct: 376 GKGVPPMNDSEKSLPSNTSSTIQMPDLLDTSDAGDYGGTDKSLEVENLSSVPLVDDLFGG 435

Query: 421 GVNTVASTSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNSQVSNENKRPASEQKNE 480
           G+NTV STSELKNDDDPFSDV F TT+  ENPDD+FSGMN +N+QV++ENK+P SEQKNE
Sbjct: 436 GLNTVTSTSELKNDDDPFSDVLFHTTETRENPDDIFSGMNFENNQVTDENKKPTSEQKNE 495

Query: 481 HGVFDVLGSSSEAAVQEHTRKDVNDLMSGLSIHEDGLKSNDIGDSKDSLSESLYSVSGQP 540
            GVFD+ GSSSE AVQEH RKDV DLMSGLSIHED LK+ D GDSKDSLSESL+SVS QP
Sbjct: 496 PGVFDIFGSSSEPAVQEHARKDVIDLMSGLSIHEDALKNKDKGDSKDSLSESLFSVSSQP 555

Query: 541 NHQYQTKDSSVNGIYSSPMVGTNMNAAFFPGMTYLPSGMMFNPAFSSRPMGYAPTGNFFA 600
           NHQ Q    S+ G YSSPMVGTNMNA FFPGM YLPSGMMFNPAFSS+PMGYAPTGNFF 
Sbjct: 556 NHQNQVPHDSLTGTYSSPMVGTNMNATFFPGMPYLPSGMMFNPAFSSQPMGYAPTGNFFT 615

Query: 601 QQNLVSAMSNYQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVMNSSKKEDT 660
           QQ L+SAMSNYQQFGNP+LQSSG     GGYSSPLPDIFQPNLA Q  SSVMNSSKKEDT
Sbjct: 616 QQQLLSAMSNYQQFGNPNLQSSG-----GGYSSPLPDIFQPNLAAQSPSSVMNSSKKEDT 675

Query: 661 RAFDFISEHIAAARDPKRVV 674
           RAFDFIS+H+AAARDPKRVV
Sbjct: 676 RAFDFISDHVAAARDPKRVV 685

BLAST of CmoCh01G001060 vs. ExPASy TrEMBL
Match: A0A0A0L8E2 (VHS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G462690 PE=4 SV=1)

HSP 1 Score: 1072.8 bits (2773), Expect = 5.0e-310
Identity = 568/682 (83.28%), Postives = 606/682 (88.86%), Query Frame = 0

Query: 1   MIDAATSDEDKVTPVYKLEEISEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK 60
           MIDAATSDEDKVTPVYKLEEI EVLRSSHVSIVKEFSEFILKRLEHKSP+VKQKALRLIK
Sbjct: 16  MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSPVVKQKALRLIK 75

Query: 61  YGIGKSGVEFRREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRDTAQDAISAIFAEEDN 120
           Y +GKSGVEFRREMQR+SVAVRQL HYKGQPDPLKGDALNKAVRDTA +AIS+IFAEEDN
Sbjct: 76  YAVGKSGVEFRREMQRNSVAVRQLFHYKGQPDPLKGDALNKAVRDTAHEAISSIFAEEDN 135

Query: 121 RPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQGLSNLAQGHSSRKNGT 180
           +PAPSENLN RIQGFGNSNYEPP EDKKSFLSEVVGLGSASIKQGLSN AQGHSSRKNGT
Sbjct: 136 KPAPSENLNRRIQGFGNSNYEPPPEDKKSFLSEVVGLGSASIKQGLSNFAQGHSSRKNGT 195

Query: 181 SGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSMISGPWNPDSWANKVEATNGNLS 240
           S HRG NLQRSLTTEMEYDNRYEPVEYGRETLGTSKS  SG WN DS       +NG+ S
Sbjct: 196 SSHRGINLQRSLTTEMEYDNRYEPVEYGRETLGTSKSTTSGTWNQDS-----RVSNGSPS 255

Query: 241 SGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAMALSSALESKLKSPSW 300
           SGSSE KTRE+RLL+TIATAGGVR+QPTRD+IQAFLVEA KLDA+ALS+ALE+KLKSPSW
Sbjct: 256 SGSSESKTREDRLLDTIATAGGVRLQPTRDSIQAFLVEAVKLDALALSNALETKLKSPSW 315

Query: 301 QVRFKALCILESIVRKNDDDHFSIVASYFSENQDAVIGCSESPQASLREKASKVMPLLDG 360
           QVRFKALCILESIVR+NDDD FSIV SYFSENQ+AVIGCSESPQASLREKA+KVMPLLDG
Sbjct: 316 QVRFKALCILESIVRRNDDDQFSIVTSYFSENQEAVIGCSESPQASLREKATKVMPLLDG 375

Query: 361 GKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAG-------VLEAENLSKTPLVDVLFGD 420
           GKGVPS+N  EKS P+N SSTIQMPDLIDTSDAG        +E ENLS TPLVD LFGD
Sbjct: 376 GKGVPSINVYEKSLPSNTSSTIQMPDLIDTSDAGDFSGTNKSVEVENLSSTPLVDDLFGD 435

Query: 421 GVNTVASTSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNSQVSNENKRPASEQKNE 480
           G+NTV STSELKNDDDPFSDVSF T +  ENPDDLFSGMN DN+QVSNENK+ A E KNE
Sbjct: 436 GLNTVTSTSELKNDDDPFSDVSFHTIETRENPDDLFSGMNFDNNQVSNENKKAALEPKNE 495

Query: 481 HGVFDVLGSSSEAAVQEHTRKDVNDLMSGLSIHEDGLKSNDIGDSKDSLSESLYSVSGQP 540
            GVFD+ GSSSE AVQEH RKDVNDLMSGLSIHED LK  D GDSKDSLSESL+S S QP
Sbjct: 496 PGVFDIFGSSSEPAVQEHARKDVNDLMSGLSIHEDILKGKDKGDSKDSLSESLFSASAQP 555

Query: 541 NHQYQTKDSSVNGIYSSPMVGTNMNAAFFPGMTYLPSGMMFNPAFSSRPMGYAPTGNFFA 600
           NHQ Q    S+NGIYSSPM G+NMNAAFFPGMTYLPSGM+FNPAFSS+PM YA TGNFF 
Sbjct: 556 NHQNQVSQDSLNGIYSSPMAGSNMNAAFFPGMTYLPSGMVFNPAFSSQPMAYAATGNFFT 615

Query: 601 QQNLVSAMSNYQQFGNPHLQSS--GGSAGNGGYSSPLPDIFQPNLATQPSSSVMNSSKKE 660
           QQ L+SAMSNYQQFGNP+LQS+  GG  G+GGYSSP PDIFQPNLA Q S+SVMNSSKKE
Sbjct: 616 QQQLLSAMSNYQQFGNPNLQSNSGGGGVGSGGYSSPFPDIFQPNLAAQSSTSVMNSSKKE 675

Query: 661 DTRAFDFISEHIAAARDPKRVV 674
           DTRAFDFIS+H+AAARDPKRVV
Sbjct: 676 DTRAFDFISDHVAAARDPKRVV 692

BLAST of CmoCh01G001060 vs. NCBI nr
Match: XP_022949030.1 (VHS domain-containing protein At3g16270-like [Cucurbita moschata])

HSP 1 Score: 1291.9 bits (3342), Expect = 0.0e+00
Identity = 673/673 (100.00%), Postives = 673/673 (100.00%), Query Frame = 0

Query: 1   MIDAATSDEDKVTPVYKLEEISEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK 60
           MIDAATSDEDKVTPVYKLEEISEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK
Sbjct: 16  MIDAATSDEDKVTPVYKLEEISEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK 75

Query: 61  YGIGKSGVEFRREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRDTAQDAISAIFAEEDN 120
           YGIGKSGVEFRREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRDTAQDAISAIFAEEDN
Sbjct: 76  YGIGKSGVEFRREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRDTAQDAISAIFAEEDN 135

Query: 121 RPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQGLSNLAQGHSSRKNGT 180
           RPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQGLSNLAQGHSSRKNGT
Sbjct: 136 RPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQGLSNLAQGHSSRKNGT 195

Query: 181 SGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSMISGPWNPDSWANKVEATNGNLS 240
           SGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSMISGPWNPDSWANKVEATNGNLS
Sbjct: 196 SGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSMISGPWNPDSWANKVEATNGNLS 255

Query: 241 SGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAMALSSALESKLKSPSW 300
           SGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAMALSSALESKLKSPSW
Sbjct: 256 SGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAMALSSALESKLKSPSW 315

Query: 301 QVRFKALCILESIVRKNDDDHFSIVASYFSENQDAVIGCSESPQASLREKASKVMPLLDG 360
           QVRFKALCILESIVRKNDDDHFSIVASYFSENQDAVIGCSESPQASLREKASKVMPLLDG
Sbjct: 316 QVRFKALCILESIVRKNDDDHFSIVASYFSENQDAVIGCSESPQASLREKASKVMPLLDG 375

Query: 361 GKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAGVLEAENLSKTPLVDVLFGDGVNTVAS 420
           GKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAGVLEAENLSKTPLVDVLFGDGVNTVAS
Sbjct: 376 GKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAGVLEAENLSKTPLVDVLFGDGVNTVAS 435

Query: 421 TSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNSQVSNENKRPASEQKNEHGVFDVL 480
           TSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNSQVSNENKRPASEQKNEHGVFDVL
Sbjct: 436 TSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNSQVSNENKRPASEQKNEHGVFDVL 495

Query: 481 GSSSEAAVQEHTRKDVNDLMSGLSIHEDGLKSNDIGDSKDSLSESLYSVSGQPNHQYQTK 540
           GSSSEAAVQEHTRKDVNDLMSGLSIHEDGLKSNDIGDSKDSLSESLYSVSGQPNHQYQTK
Sbjct: 496 GSSSEAAVQEHTRKDVNDLMSGLSIHEDGLKSNDIGDSKDSLSESLYSVSGQPNHQYQTK 555

Query: 541 DSSVNGIYSSPMVGTNMNAAFFPGMTYLPSGMMFNPAFSSRPMGYAPTGNFFAQQNLVSA 600
           DSSVNGIYSSPMVGTNMNAAFFPGMTYLPSGMMFNPAFSSRPMGYAPTGNFFAQQNLVSA
Sbjct: 556 DSSVNGIYSSPMVGTNMNAAFFPGMTYLPSGMMFNPAFSSRPMGYAPTGNFFAQQNLVSA 615

Query: 601 MSNYQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVMNSSKKEDTRAFDFIS 660
           MSNYQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVMNSSKKEDTRAFDFIS
Sbjct: 616 MSNYQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVMNSSKKEDTRAFDFIS 675

Query: 661 EHIAAARDPKRVV 674
           EHIAAARDPKRVV
Sbjct: 676 EHIAAARDPKRVV 688

BLAST of CmoCh01G001060 vs. NCBI nr
Match: KAG6606757.1 (Protein MODIFIED TRANSPORT TO THE VACUOLE 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1263.4 bits (3268), Expect = 0.0e+00
Identity = 657/673 (97.62%), Postives = 663/673 (98.51%), Query Frame = 0

Query: 1   MIDAATSDEDKVTPVYKLEEISEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK 60
           MIDAATSDEDKVTPVYKLEEI EVLRSSHVSIVKEFSEFILKRLEHKSPIV+QKALRLIK
Sbjct: 16  MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSPIVQQKALRLIK 75

Query: 61  YGIGKSGVEFRREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRDTAQDAISAIFAEEDN 120
           YG+GKSGVEFRREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRDTAQDAISAIFAEEDN
Sbjct: 76  YGVGKSGVEFRREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRDTAQDAISAIFAEEDN 135

Query: 121 RPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQGLSNLAQGHSSRKNGT 180
           RPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQGLSNLAQGHSSRKNGT
Sbjct: 136 RPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQGLSNLAQGHSSRKNGT 195

Query: 181 SGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSMISGPWNPDSWANKVEATNGNLS 240
           SGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKS I+GPWN DSWANKVEATNGNLS
Sbjct: 196 SGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSTITGPWNQDSWANKVEATNGNLS 255

Query: 241 SGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAMALSSALESKLKSPSW 300
           SGSSER+TREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAMALSSALESKLKSPSW
Sbjct: 256 SGSSERRTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAMALSSALESKLKSPSW 315

Query: 301 QVRFKALCILESIVRKNDDDHFSIVASYFSENQDAVIGCSESPQASLREKASKVMPLLDG 360
           QVRFKALC+LESIVRKNDDDHFSIVASYFSENQDAVIGCSESPQASLREKASKVMPLLDG
Sbjct: 316 QVRFKALCVLESIVRKNDDDHFSIVASYFSENQDAVIGCSESPQASLREKASKVMPLLDG 375

Query: 361 GKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAGVLEAENLSKTPLVDVLFGDGVNTVAS 420
           GKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAG LEAENLSKTPLVD LFGDGVNTVAS
Sbjct: 376 GKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAGALEAENLSKTPLVDDLFGDGVNTVAS 435

Query: 421 TSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNSQVSNENKRPASEQKNEHGVFDVL 480
           TSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNSQVSNENKRPASEQKNEHGVFDV 
Sbjct: 436 TSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNSQVSNENKRPASEQKNEHGVFDVF 495

Query: 481 GSSSEAAVQEHTRKDVNDLMSGLSIHEDGLKSNDIGDSKDSLSESLYSVSGQPNHQYQTK 540
           GSSSEA VQEHTRKDVNDLMSGLSIHEDGLKSNDIGDSKDSLSESLYSVS QPNHQYQTK
Sbjct: 496 GSSSEAGVQEHTRKDVNDLMSGLSIHEDGLKSNDIGDSKDSLSESLYSVSSQPNHQYQTK 555

Query: 541 DSSVNGIYSSPMVGTNMNAAFFPGMTYLPSGMMFNPAFSSRPMGYAPTGNFFAQQNLVSA 600
           DSSVNGIYSSPMVGTNMNAAFFPGMTYLPSGMMFNPAFSS+P GYAPTGNFFAQQNLVSA
Sbjct: 556 DSSVNGIYSSPMVGTNMNAAFFPGMTYLPSGMMFNPAFSSQPRGYAPTGNFFAQQNLVSA 615

Query: 601 MSNYQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVMNSSKKEDTRAFDFIS 660
           MSNYQQFGNP LQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVMNSSKKEDTRAFDFIS
Sbjct: 616 MSNYQQFGNPRLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVMNSSKKEDTRAFDFIS 675

Query: 661 EHIAAARDPKRVV 674
           EHIAAARDPKRVV
Sbjct: 676 EHIAAARDPKRVV 688

BLAST of CmoCh01G001060 vs. NCBI nr
Match: XP_023524865.1 (VHS domain-containing protein At3g16270-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1255.0 bits (3246), Expect = 0.0e+00
Identity = 654/673 (97.18%), Postives = 658/673 (97.77%), Query Frame = 0

Query: 1   MIDAATSDEDKVTPVYKLEEISEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK 60
           MIDAATSDEDKVTPVYKLEEI EVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRL K
Sbjct: 16  MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLTK 75

Query: 61  YGIGKSGVEFRREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRDTAQDAISAIFAEEDN 120
           Y +GKSGVEF REMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRDTAQDAISAIFAEEDN
Sbjct: 76  YAVGKSGVEFGREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRDTAQDAISAIFAEEDN 135

Query: 121 RPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQGLSNLAQGHSSRKNGT 180
           RPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQGLSNLAQGHSSRKNGT
Sbjct: 136 RPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQGLSNLAQGHSSRKNGT 195

Query: 181 SGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSMISGPWNPDSWANKVEATNGNLS 240
           SGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKS ISGPWNPDSWANKVEATNGNLS
Sbjct: 196 SGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSTISGPWNPDSWANKVEATNGNLS 255

Query: 241 SGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAMALSSALESKLKSPSW 300
           SGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAMALSSALESKLKSPSW
Sbjct: 256 SGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAMALSSALESKLKSPSW 315

Query: 301 QVRFKALCILESIVRKNDDDHFSIVASYFSENQDAVIGCSESPQASLREKASKVMPLLDG 360
           QVRFKALC+LESIVRK DDDHFSIVASYFSENQDAVIGCSESPQASLREKASKVMPLLDG
Sbjct: 316 QVRFKALCVLESIVRKKDDDHFSIVASYFSENQDAVIGCSESPQASLREKASKVMPLLDG 375

Query: 361 GKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAGVLEAENLSKTPLVDVLFGDGVNTVAS 420
           GKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAGVLE ENLSKTPLVD LFGDGVNTV S
Sbjct: 376 GKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAGVLEVENLSKTPLVDDLFGDGVNTVTS 435

Query: 421 TSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNSQVSNENKRPASEQKNEHGVFDVL 480
           TSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDN QVSNENKRPASEQKNEHGVF V 
Sbjct: 436 TSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNGQVSNENKRPASEQKNEHGVFYVF 495

Query: 481 GSSSEAAVQEHTRKDVNDLMSGLSIHEDGLKSNDIGDSKDSLSESLYSVSGQPNHQYQTK 540
           GSSSEAAVQEHTRKDVNDLMSGLSIHE+ LKSNDIGDSKDSLSESLYSVS QPNHQYQTK
Sbjct: 496 GSSSEAAVQEHTRKDVNDLMSGLSIHENALKSNDIGDSKDSLSESLYSVSSQPNHQYQTK 555

Query: 541 DSSVNGIYSSPMVGTNMNAAFFPGMTYLPSGMMFNPAFSSRPMGYAPTGNFFAQQNLVSA 600
           DSSVNGIYSSPMVGTNMNAAF PGMTYLPSGMMFNPAFSS+PMGYAPTGNFFAQQNLVSA
Sbjct: 556 DSSVNGIYSSPMVGTNMNAAFLPGMTYLPSGMMFNPAFSSQPMGYAPTGNFFAQQNLVSA 615

Query: 601 MSNYQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVMNSSKKEDTRAFDFIS 660
           MSNYQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVMNSSKKEDTRAFDFIS
Sbjct: 616 MSNYQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVMNSSKKEDTRAFDFIS 675

Query: 661 EHIAAARDPKRVV 674
           EHIAAARDPKRVV
Sbjct: 676 EHIAAARDPKRVV 688

BLAST of CmoCh01G001060 vs. NCBI nr
Match: XP_022997847.1 (VHS domain-containing protein At3g16270-like [Cucurbita maxima])

HSP 1 Score: 1248.0 bits (3228), Expect = 0.0e+00
Identity = 651/673 (96.73%), Postives = 659/673 (97.92%), Query Frame = 0

Query: 1   MIDAATSDEDKVTPVYKLEEISEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK 60
           MIDAATSDEDKVTPVYKLEEI EVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK
Sbjct: 16  MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK 75

Query: 61  YGIGKSGVEFRREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRDTAQDAISAIFAEEDN 120
           YG+GKSGVEFRREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRDTAQDAISAIFAEEDN
Sbjct: 76  YGVGKSGVEFRREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRDTAQDAISAIFAEEDN 135

Query: 121 RPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQGLSNLAQGHSSRKNGT 180
           RPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQGLSNLAQGHSSRKNGT
Sbjct: 136 RPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQGLSNLAQGHSSRKNGT 195

Query: 181 SGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSMISGPWNPDSWANKVEATNGNLS 240
           SGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKS ISGPWNPDSWANKVEATNGNLS
Sbjct: 196 SGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSTISGPWNPDSWANKVEATNGNLS 255

Query: 241 SGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAMALSSALESKLKSPSW 300
           SGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAMALSSALESKLKSPSW
Sbjct: 256 SGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAMALSSALESKLKSPSW 315

Query: 301 QVRFKALCILESIVRKNDDDHFSIVASYFSENQDAVIGCSESPQASLREKASKVMPLLDG 360
           QVRFKALC+LESIVRKNDDDHFSIVASYFSENQDAVIGCSESPQASLREKASKVMPLLDG
Sbjct: 316 QVRFKALCVLESIVRKNDDDHFSIVASYFSENQDAVIGCSESPQASLREKASKVMPLLDG 375

Query: 361 GKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAGVLEAENLSKTPLVDVLFGDGVNTVAS 420
           GKGVPSMN SEKS PNNPSSTIQMPDLIDTSDAGVLE ENLSKTPLVD LFGDGVNT+ S
Sbjct: 376 GKGVPSMNASEKSLPNNPSSTIQMPDLIDTSDAGVLEVENLSKTPLVDDLFGDGVNTITS 435

Query: 421 TSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNSQVSNENKRPASEQKNEHGVFDVL 480
           TSELKNDDDPFSDVSFQTTD+GENPDDLFSGM VDNSQVSNE+KRPASEQKNEHGVFDV 
Sbjct: 436 TSELKNDDDPFSDVSFQTTDDGENPDDLFSGMIVDNSQVSNESKRPASEQKNEHGVFDVF 495

Query: 481 GSSSEAAVQEHTRKDVNDLMSGLSIHEDGLKSNDIGDSKDSLSESLYSVSGQPNHQYQTK 540
           GSSSEAAVQEHTRKDVN+LMSGLSIHEDGLKSNDIGDSKDSLSESLYSVS QPNHQYQTK
Sbjct: 496 GSSSEAAVQEHTRKDVNELMSGLSIHEDGLKSNDIGDSKDSLSESLYSVSRQPNHQYQTK 555

Query: 541 DSSVNGIYSSPMVGTNMNAAFFPGMTYLPSGMMFNPAFSSRPMGYAPTGNFFAQQNLVSA 600
           DSSVNGIYSSPMVGTNMNAA FPG TYLPSGMMFNPAFSS+PMGYAPTGNFFAQQNL SA
Sbjct: 556 DSSVNGIYSSPMVGTNMNAALFPGRTYLPSGMMFNPAFSSQPMGYAPTGNFFAQQNLPSA 615

Query: 601 MSNYQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVMNSSKKEDTRAFDFIS 660
           MSN QQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVMNSSKKEDTRAFDFIS
Sbjct: 616 MSNSQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVMNSSKKEDTRAFDFIS 675

Query: 661 EHIAAARDPKRVV 674
           EHIAAARDPK+VV
Sbjct: 676 EHIAAARDPKQVV 688

BLAST of CmoCh01G001060 vs. NCBI nr
Match: KAG7036470.1 (VHS domain-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1142.5 bits (2954), Expect = 0.0e+00
Identity = 607/673 (90.19%), Postives = 613/673 (91.08%), Query Frame = 0

Query: 1   MIDAATSDEDKVTPVYKLEEISEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK 60
           MIDAATSDEDKVT VYKLEEI EVLRSSHVSIVKEFSEFILKRLEHKSPIV+QKALRLIK
Sbjct: 16  MIDAATSDEDKVTLVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSPIVQQKALRLIK 75

Query: 61  YGIGKSGVEFRREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRDTAQDAISAIFAEEDN 120
           YG+GKSGVEFRREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRDTAQDAISAIFAEEDN
Sbjct: 76  YGVGKSGVEFRREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRDTAQDAISAIFAEEDN 135

Query: 121 RPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQGLSNLAQGHSSRKNGT 180
           RPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQGLSNLAQGHSSRKNGT
Sbjct: 136 RPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQGLSNLAQGHSSRKNGT 195

Query: 181 SGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSMISGPWNPDSWANKVEATNGNLS 240
           SGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKS I+GPWN DSWANKVEATNGNLS
Sbjct: 196 SGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSTITGPWNQDSWANKVEATNGNLS 255

Query: 241 SGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAMALSSALESKLKSPSW 300
           SGSSER+TREERLLETIATAGGVRIQPTRDAIQAFLVEAA                    
Sbjct: 256 SGSSERRTREERLLETIATAGGVRIQPTRDAIQAFLVEAA-------------------- 315

Query: 301 QVRFKALCILESIVRKNDDDHFSIVASYFSENQDAVIGCSESPQASLREKASKVMPLLDG 360
                                        +ENQDAVIGCSESPQASLREKASKVMPLLDG
Sbjct: 316 -----------------------------NENQDAVIGCSESPQASLREKASKVMPLLDG 375

Query: 361 GKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAGVLEAENLSKTPLVDVLFGDGVNTVAS 420
           GKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAG LEAENLSKTPLVD LFGDGVNTVAS
Sbjct: 376 GKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAGALEAENLSKTPLVDDLFGDGVNTVAS 435

Query: 421 TSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNSQVSNENKRPASEQKNEHGVFDVL 480
           TSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNSQVSNENKRPASEQKNEHGVFDV 
Sbjct: 436 TSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNSQVSNENKRPASEQKNEHGVFDVF 495

Query: 481 GSSSEAAVQEHTRKDVNDLMSGLSIHEDGLKSNDIGDSKDSLSESLYSVSGQPNHQYQTK 540
           GSSSEA VQEHTRKDVNDLMSGLSIHEDGLKSNDIGDSKDSLSESLYSVS QPNHQYQTK
Sbjct: 496 GSSSEAGVQEHTRKDVNDLMSGLSIHEDGLKSNDIGDSKDSLSESLYSVSSQPNHQYQTK 555

Query: 541 DSSVNGIYSSPMVGTNMNAAFFPGMTYLPSGMMFNPAFSSRPMGYAPTGNFFAQQNLVSA 600
           DSSVNGIYSSPMVGTNMNAAFFPGMTYLPSGMMFNPAFSS+P GYAPTGNFFAQQNLVSA
Sbjct: 556 DSSVNGIYSSPMVGTNMNAAFFPGMTYLPSGMMFNPAFSSQPRGYAPTGNFFAQQNLVSA 615

Query: 601 MSNYQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVMNSSKKEDTRAFDFIS 660
           MSNYQQFGNP LQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVMNSSKKEDTRAFDFIS
Sbjct: 616 MSNYQQFGNPRLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVMNSSKKEDTRAFDFIS 639

Query: 661 EHIAAARDPKRVV 674
           EHIAAARDPKRVV
Sbjct: 676 EHIAAARDPKRVV 639

BLAST of CmoCh01G001060 vs. TAIR 10
Match: AT3G16270.1 (ENTH/VHS family protein )

HSP 1 Score: 627.5 bits (1617), Expect = 1.3e-179
Identity = 375/692 (54.19%), Postives = 476/692 (68.79%), Query Frame = 0

Query: 1   MIDAATSDEDKVTPVYKLEEISEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK 60
           MIDA TSDEDKV PVYKLEEI ++LRSSHVSIVKEFSEFILKRL++KSPIVKQKALRLIK
Sbjct: 16  MIDAVTSDEDKVAPVYKLEEICDLLRSSHVSIVKEFSEFILKRLDNKSPIVKQKALRLIK 75

Query: 61  YGIGKSGVEFRREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRDTAQDAISAIFAEED- 120
           Y +GKSG EFRREMQR+SVAVR L HYKG PDPLKGDALNKAVR+TA + ISAIF+EE+ 
Sbjct: 76  YAVGKSGSEFRREMQRNSVAVRNLFHYKGHPDPLKGDALNKAVRETAHETISAIFSEENG 135

Query: 121 NRPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQGLSNLAQGHSSRK-- 180
            +PA  E++N RI+GFGN+N++ P+ D KSFLSEVVG+GSASIKQG+SN AQGH  +K  
Sbjct: 136 TKPAAPESINRRIEGFGNTNFQVPSNDNKSFLSEVVGIGSASIKQGISNFAQGHLPKKNE 195

Query: 181 NGTSGHRGPNLQRSLTTEMEYDNRYEPVEYGRE-TLGTSKSMISGPWNPDSWANKVEATN 240
           NG+S +RGPNL RSLT E E  +RY+PV+ G++   GTSK+   G     SW +     +
Sbjct: 196 NGSSSYRGPNLHRSLTMENENFSRYDPVKLGKDGNYGTSKNTTGG-----SWGHASGEAS 255

Query: 241 GNLSSGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAMALSSALESKLK 300
            + +S   E KTREE+LLETI T+GGVR+QPTRDA+  F++EAAK+DA+ALS AL+ KL 
Sbjct: 256 ESSASVRVESKTREEKLLETIVTSGGVRLQPTRDALHVFILEAAKMDAVALSIALDGKLH 315

Query: 301 SPSWQVRFKALCILESIVRKNDDDHFSIVASYFSENQDAVIGCSESPQASLREKASKVMP 360
           SP WQVR KALC+LE+I+RK +D++FSIV +YFSEN DA+  C+ESPQ+SLREKA+KV+ 
Sbjct: 316 SPMWQVRMKALCVLEAILRKKEDENFSIVHTYFSENLDAIQRCAESPQSSLREKANKVLS 375

Query: 361 LLDGGKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDA----GVLEAENLSKT-----PLV 420
           LL+GG+    M+ S+ +      + + +PDLIDT D+      L A +   T     PL+
Sbjct: 376 LLNGGQSSGLMSSSDNTVKR--EAAVDLPDLIDTGDSDDTLNNLNAIDTGSTVATAGPLM 435

Query: 421 -DVLFGDGVNTVASTSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNSQVSNENKRP 480
            D  FGD  +   S+SE K DDDPF+DVSF   +  E+ DDLFSGM V         K  
Sbjct: 436 DDDWFGDSSDIGLSSSEKKTDDDPFADVSFHPNEEKESADDLFSGMTVG-------EKSA 495

Query: 481 ASEQKNEHGVFDVLGSSSEAAVQEHTRKDVNDLMSGLSIHEDGLKSNDIGDSKDSLSESL 540
           A    +   +FD+ GS+++   +    K++NDLM   SI E+   SN  G S  +L + L
Sbjct: 496 AVGGNHVPDLFDMFGSTAKLEAEPKDAKNINDLMGSFSIDEN--NSNQKGSSSSTLPQDL 555

Query: 541 YSVSGQPNHQYQTKDSSVNGIYSSPMVGTNMNAAFFPGMT--YLPSGMMFNPAFSSRPMG 600
           +++    +H  Q  ++ V GI  S   G   N     G+     P GMM NPAF+S+P+ 
Sbjct: 556 FAMPSTTSH--QAPENPVGGILGSQNPGFIQNTMLPGGVMPFNFPQGMMMNPAFASQPLN 615

Query: 601 YAPTGNFFA-QQNLVSAMSNYQQFGNPHLQSSGG--SAG-NGGYSSPLPDIFQPNLATQP 660
           YA   +  A QQ  +  MSN+QQFGN + Q SG   S G +GG  S LPDIFQPN   Q 
Sbjct: 616 YAAMASLLAQQQQYLGNMSNFQQFGNLNAQGSGNVLSMGTSGGNQSALPDIFQPNFGNQA 675

Query: 661 SSSVMNSSKKEDTRAFDFISEHIAAARDPKRV 673
            +S MN SKKEDTRAFDFIS+H+ +ARD KRV
Sbjct: 676 PTSTMNGSKKEDTRAFDFISDHLTSARDTKRV 689