CmoCh01G000270 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh01G000270
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	D-3-phosphoglycerate dehydrogenase
Location	Cmo_Chr01: 99919 .. 100870 (+)
RNA-Seq Expression	CmoCh01G000270
Synteny	CmoCh01G000270

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGATTAAGTGGTTCGTAAATGATGTTTAATTACTCCCTAAATTTCAGCGCTCCTATTTTTAAGAATCCCAACCCCATTTCTCCACCATCGACATAAACTCAACCCTGTTTCCTTCACTCTCACCTTCCAACTCCATCTCCAATGGCTTCCTCCTCTGCAAACGCCCTCTTTTCCTCTTCCCTCGCACCCTCCATTCCCAAATCCCCTCTTCTCTCCATCTCCAACTCCTCTGTTTCTCTCAACCTCCTCCCCACTCTTCATTCTTCTTCTTCCGCTTCTTCTTCTCGCTGCAATTTTATTCCAATCCGCAGCGTTTTGAAGACGGTGGAAACCTTCGAGCTTCACACCTCCGATCTCGCCGACCCCACTTTCCCCAACACAAGATCCACCATTTTGGTCGCCGAGAAACTTGGAGAGGCCGGTCTGAAGCTTCTCCGGAGCTTCGGCGACGTTGACTGCGCTTACGACCTCTCGCCGGAACAACTCTGCGCCAAGATCTCTTCCTGCGACGCCTTAATTGTTCGAAGTGGCACCACGGTCAATCGACAGCTCTTTGAAGCGGCGAAAGGGCGGTTGAAGGTTGTCGGAAGAGCCGGTGTCGGTATTGACAATGTGGATCTGCAAGCCGCCACTGAGTTCGGTTGTCTTGTCGTGAATGCTCCGACTGCCAACACCATCGCCGCCGCCGAACATGGAATTGCGTTGCTTACTGCCATGGCTCGTAACGTCGCTCAAGCTGATGCCTCCATGAAAGCAGGTCGGTAATGGATGCTCTGTTTTGCGACCCAAAATTCAAAAACACCCTTTTGTTTTAACGATTAGATCTGCTAATAATTAGTAACCCAGGTTCCAAGCTCAAGAATTCTTTTGAAAAAAAATGATCTTGAACTGTTGTTCTTGGTTTTAAGTTGAAAAATCTGAGCAGGATTCTACTGAAAAAGCCTCTGCTTAA

mRNA sequence

AGATTAAGTGGTTCGTAAATGATGTTTAATTACTCCCTAAATTTCAGCGCTCCTATTTTTAAGAATCCCAACCCCATTTCTCCACCATCGACATAAACTCAACCCTGTTTCCTTCACTCTCACCTTCCAACTCCATCTCCAATGGCTTCCTCCTCTGCAAACGCCCTCTTTTCCTCTTCCCTCGCACCCTCCATTCCCAAATCCCCTCTTCTCTCCATCTCCAACTCCTCTGTTTCTCTCAACCTCCTCCCCACTCTTCATTCTTCTTCTTCCGCTTCTTCTTCTCGCTGCAATTTTATTCCAATCCGCAGCGTTTTGAAGACGGTGGAAACCTTCGAGCTTCACACCTCCGATCTCGCCGACCCCACTTTCCCCAACACAAGATCCACCATTTTGGTCGCCGAGAAACTTGGAGAGGCCGGTCTGAAGCTTCTCCGGAGCTTCGGCGACGTTGACTGCGCTTACGACCTCTCGCCGGAACAACTCTGCGCCAAGATCTCTTCCTGCGACGCCTTAATTGTTCGAAGTGGCACCACGGTCAATCGACAGCTCTTTGAAGCGGCGAAAGGGCGGTTGAAGGTTGTCGGAAGAGCCGGTGTCGGTATTGACAATGTGGATCTGCAAGCCGCCACTGAGTTCGGTTGTCTTGTCGTGAATGCTCCGACTGCCAACACCATCGCCGCCGCCGAACATGGAATTGCGTTGCTTACTGCCATGGCTCGTAACGTCGCTCAAGCTGATGCCTCCATGAAAGCAGTTGAAAAATCTGAGCAGGATTCTACTGAAAAAGCCTCTGCTTAA

Coding sequence (CDS)

ATGGCTTCCTCCTCTGCAAACGCCCTCTTTTCCTCTTCCCTCGCACCCTCCATTCCCAAATCCCCTCTTCTCTCCATCTCCAACTCCTCTGTTTCTCTCAACCTCCTCCCCACTCTTCATTCTTCTTCTTCCGCTTCTTCTTCTCGCTGCAATTTTATTCCAATCCGCAGCGTTTTGAAGACGGTGGAAACCTTCGAGCTTCACACCTCCGATCTCGCCGACCCCACTTTCCCCAACACAAGATCCACCATTTTGGTCGCCGAGAAACTTGGAGAGGCCGGTCTGAAGCTTCTCCGGAGCTTCGGCGACGTTGACTGCGCTTACGACCTCTCGCCGGAACAACTCTGCGCCAAGATCTCTTCCTGCGACGCCTTAATTGTTCGAAGTGGCACCACGGTCAATCGACAGCTCTTTGAAGCGGCGAAAGGGCGGTTGAAGGTTGTCGGAAGAGCCGGTGTCGGTATTGACAATGTGGATCTGCAAGCCGCCACTGAGTTCGGTTGTCTTGTCGTGAATGCTCCGACTGCCAACACCATCGCCGCCGCCGAACATGGAATTGCGTTGCTTACTGCCATGGCTCGTAACGTCGCTCAAGCTGATGCCTCCATGAAAGCAGTTGAAAAATCTGAGCAGGATTCTACTGAAAAAGCCTCTGCTTAA

Protein sequence

MASSSANALFSSSLAPSIPKSPLLSISNSSVSLNLLPTLHSSSSASSSRCNFIPIRSVLKTVETFELHTSDLADPTFPNTRSTILVAEKLGEAGLKLLRSFGDVDCAYDLSPEQLCAKISSCDALIVRSGTTVNRQLFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAVEKSEQDSTEKASA

Homology

[Close]

BLAST of CmoCh01G000270 vs. ExPASy Swiss-Prot
Match: O04130 (D-3-phosphoglycerate dehydrogenase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PGDH2 PE=1 SV=2)

HSP 1 Score: 203.0 bits (515), Expect = 3.5e-51
Identity = 114/161 (70.81%), Postives = 125/161 (77.64%), Query Frame = 0


Query: 55  IRSVLKTVETFEL----------HTSDLADPTFPNTRSTILVAEKLGEAGLKLLRSFGDV 114
           I + LKTVE   L            SD  +PT P  R  ILV EKLGEAG+ LLR FGDV
Sbjct: 48  ISAALKTVEQTTLTEDNRFSTVGSDSDEYNPTLPKPR--ILVTEKLGEAGVNLLREFGDV 107

Query: 115 DCAYDLSPEQLCAKISSCDALIVRSGTTVNRQLFEAAKGRLKVVGRAGVGIDNVDLQAAT 174
           DC+YDLSPE L  K++  DALIVRSGT V R++FEAAKGRLKVVGRAGVGIDNVDLQAAT
Sbjct: 108 DCSYDLSPEDLKKKVAESDALIVRSGTKVTREVFEAAKGRLKVVGRAGVGIDNVDLQAAT 167

Query: 175 EFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKA 206
           E GCLVVNAPTANT+AAAEHGIALL +MARNVAQADAS+KA
Sbjct: 168 EHGCLVVNAPTANTVAAAEHGIALLASMARNVAQADASIKA 206

[Close]

BLAST of CmoCh01G000270 vs. ExPASy Swiss-Prot
Match: Q9LT69 (D-3-phosphoglycerate dehydrogenase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PGDH3 PE=1 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 1.2e-48
Identity = 110/176 (62.50%), Postives = 133/176 (75.57%), Query Frame = 0


Query: 30  SVSLNLLPTLHSSSSASSSRCNFIPIRSVLKTVETFELHTSDLADPTFPNTRSTILVAEK 89
           + SLNL     SSS   ++  +  PIR   + +    L TS  +       + TILV EK
Sbjct: 2   ATSLNLSSIFSSSSRLVTTPSSVFPIRQRRRII----LVTSSSSG---GGGKPTILVTEK 61

Query: 90  LGEAGLKLLRSFGDVDCAYDLSPEQLCAKISSCDALIVRSGTTVNRQLFEAAKGRLKVVG 149
           LG+AG+ LL+ + +VDC+YDLS E+LC KIS CDALIVRSGT V R +FE+++GRLKVVG
Sbjct: 62  LGQAGIDLLKKYANVDCSYDLSLEELCTKISLCDALIVRSGTKVGRDVFESSRGRLKVVG 121

Query: 150 RAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKA 206
           RAGVGIDNVDL AATE+GCLVVNAPTANT+AAAEHGIALLTAMARN+AQADAS+KA
Sbjct: 122 RAGVGIDNVDLAAATEYGCLVVNAPTANTVAAAEHGIALLTAMARNIAQADASIKA 170

[Close]

BLAST of CmoCh01G000270 vs. ExPASy Swiss-Prot
Match: O49485 (D-3-phosphoglycerate dehydrogenase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PGDH1 PE=1 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 1.4e-47
Identity = 99/132 (75.00%), Postives = 115/132 (87.12%), Query Frame = 0


Query: 76  TFPNTRSTILVAEKLGEAGLKLLRSFGDVDCAYDLSPEQLCAKISSCDALIVRSGTTVNR 135
           T   ++ TILVAEKLG+AG+KLL    +VDC+Y+++PE+L  KIS CDALIVRSGT V R
Sbjct: 56  TGDGSKPTILVAEKLGDAGIKLLEDVANVDCSYNMTPEELNIKISLCDALIVRSGTKVGR 115

Query: 136 QLFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTAMARN 195
           ++FE++ GRLKVVGRAGVGIDNVDL AATEFGCLVVNAPTANTIAAAEHGIAL+ AMARN
Sbjct: 116 EVFESSHGRLKVVGRAGVGIDNVDLSAATEFGCLVVNAPTANTIAAAEHGIALMAAMARN 175

Query: 196 VAQADASMKAVE 208
           VAQADAS+KA E
Sbjct: 176 VAQADASVKAGE 187

[Close]

BLAST of CmoCh01G000270 vs. ExPASy Swiss-Prot
Match: Q58424 (D-3-phosphoglycerate dehydrogenase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=serA PE=3 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 1.3e-24
Identity = 63/124 (50.81%), Postives = 87/124 (70.16%), Query Frame = 0


Query: 84  ILVAEKLGEAGLKLLRSFGDVDCAYDLSPEQLCAKISSCDALIVRSGTTVNRQLFEAAKG 143
           ILV + L E  +K+L   G+V+ A  L+ E+L  KI   D L+VRSGT V R + E A+ 
Sbjct: 4   ILVTDPLHEDAIKILEEVGEVEVATGLTKEELLEKIKDADVLVVRSGTKVTRDVIEKAE- 63

Query: 144 RLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADASM 203
           +LKV+GRAGVG+DN+D++AATE G +VVNAP A++I+ AE  + L+ A ARN+ QA AS+
Sbjct: 64  KLKVIGRAGVGVDNIDVEAATEKGIIVVNAPDASSISVAELTMGLMLAAARNIPQATASL 123

Query: 204 KAVE 208
           K  E
Sbjct: 124 KRGE 126

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BLAST of CmoCh01G000270 vs. ExPASy Swiss-Prot
Match: O27051 (D-3-phosphoglycerate dehydrogenase OS=Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) OX=187420 GN=serA PE=3 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.5e-22
Identity = 56/125 (44.80%), Postives = 84/125 (67.20%), Query Frame = 0


Query: 80  TRSTILVAEKLGEAGLKLLRSFGDVDCAYDLSPEQLCAKISSCDALIVRSGTTVNRQLFE 139
           +R  +L+A+ + E G+  L    +V     ++PE+L   I   DA++VRS T V R++ E
Sbjct: 2   SRMKVLIADSINEKGISELEEVAEVVVNTTITPEELLDAIKDFDAIVVRSRTKVTREVIE 61

Query: 140 AAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQA 199
           AA  RLK++ RAGVG+DNVD++AAT+ G +V+NAP + +I  AEH I L+ A+AR +A A
Sbjct: 62  AAP-RLKIIARAGVGVDNVDVKAATDRGIMVINAPESTSITVAEHSIGLMLALARKIAIA 121

Query: 200 DASMK 205
           D S+K
Sbjct: 122 DRSVK 125

[Close]

BLAST of CmoCh01G000270 vs. ExPASy TrEMBL
Match: A0A6J1GBM1 (D-3-phosphoglycerate dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111452687 PE=3 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 3.8e-101
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 0


Query: 1   MASSSANALFSSSLAPSIPKSPLLSISNSSVSLNLLPTLHSSSSASSSRCNFIPIRSVLK 60
           MASSSANALFSSSLAPSIPKSPLLSISNSSVSLNLLPTLHSSSSASSSRCNFIPIRSVLK
Sbjct: 1   MASSSANALFSSSLAPSIPKSPLLSISNSSVSLNLLPTLHSSSSASSSRCNFIPIRSVLK 60

Query: 61  TVETFELHTSDLADPTFPNTRSTILVAEKLGEAGLKLLRSFGDVDCAYDLSPEQLCAKIS 120
           TVETFELHTSDLADPTFPNTRSTILVAEKLGEAGLKLLRSFGDVDCAYDLSPEQLCAKIS
Sbjct: 61  TVETFELHTSDLADPTFPNTRSTILVAEKLGEAGLKLLRSFGDVDCAYDLSPEQLCAKIS 120

Query: 121 SCDALIVRSGTTVNRQLFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIA 180
           SCDALIVRSGTTVNRQLFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIA
Sbjct: 121 SCDALIVRSGTTVNRQLFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIA 180

Query: 181 AAEHGIALLTAMARNVAQADASMKA 206
           AAEHGIALLTAMARNVAQADASMKA
Sbjct: 181 AAEHGIALLTAMARNVAQADASMKA 205

[Close]

BLAST of CmoCh01G000270 vs. ExPASy TrEMBL
Match: A0A6J1KGG7 (D-3-phosphoglycerate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111493010 PE=3 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 6.8e-98
Identity = 199/205 (97.07%), Postives = 201/205 (98.05%), Query Frame = 0


Query: 1   MASSSANALFSSSLAPSIPKSPLLSISNSSVSLNLLPTLHSSSSASSSRCNFIPIRSVLK 60
           MASSSANALFSSSLAPSIP+SPL SISNSSVSLNLLPTLHSSS  SSSRCNFIPIRSVLK
Sbjct: 1   MASSSANALFSSSLAPSIPESPLFSISNSSVSLNLLPTLHSSSFISSSRCNFIPIRSVLK 60

Query: 61  TVETFELHTSDLADPTFPNTRSTILVAEKLGEAGLKLLRSFGDVDCAYDLSPEQLCAKIS 120
           TVET ELHTSDLADPTFPNTRSTILVAEKLGEAGLKLLRSFGDVDCAYDLSPEQLCAKIS
Sbjct: 61  TVETLELHTSDLADPTFPNTRSTILVAEKLGEAGLKLLRSFGDVDCAYDLSPEQLCAKIS 120

Query: 121 SCDALIVRSGTTVNRQLFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIA 180
           SCDALIVRSGTTVNRQ+FEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIA
Sbjct: 121 SCDALIVRSGTTVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIA 180

Query: 181 AAEHGIALLTAMARNVAQADASMKA 206
           AAEHGIALLTAMARNVAQADASMKA
Sbjct: 181 AAEHGIALLTAMARNVAQADASMKA 205

[Close]

BLAST of CmoCh01G000270 vs. ExPASy TrEMBL
Match: A0A0A0LC35 (D-3-phosphoglycerate dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_3G651740 PE=3 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 6.2e-75
Identity = 172/215 (80.00%), Postives = 185/215 (86.05%), Query Frame = 0


Query: 1   MASSSANALFSSSLAPSIPKS-----PLLSISNSSVSLNLLPTLHSSSSASSS---RCNF 60
           MASSS+N+LFSS    S  +S     PLLS S SSVSLN  P LHSSSS+SSS   RC+F
Sbjct: 1   MASSSSNSLFSSPHFTSFNRSFHSKPPLLSFSTSSVSLNPRP-LHSSSSSSSSSSNRCSF 60

Query: 61  IPIRSVLKTVET--FELHTSDLADPTFPNTRSTILVAEKLGEAGLKLLRSFGDVDCAYDL 120
           IP+RSVLKT+E+  F    SDL DPT PN RSTILVAEKLGEAGL+LLRSFG+V CAYDL
Sbjct: 61  IPLRSVLKTLESSAFVSQNSDLLDPTSPNDRSTILVAEKLGEAGLELLRSFGNVVCAYDL 120

Query: 121 SPEQLCAKISSCDALIVRSGTTVNRQLFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLV 180
           SPE+LCAKISSCDALIVRSGT VNRQ+FEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLV
Sbjct: 121 SPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLV 180

Query: 181 VNAPTANTIAAAEHGIALLTAMARNVAQADASMKA 206
           VNAPTANTIAAAEHGIALLT+MARNVAQADASMKA
Sbjct: 181 VNAPTANTIAAAEHGIALLTSMARNVAQADASMKA 214

[Close]

BLAST of CmoCh01G000270 vs. ExPASy TrEMBL
Match: A0A5A7VDA4 (D-3-phosphoglycerate dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold90G00680 PE=3 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 2.3e-74
Identity = 171/215 (79.53%), Postives = 184/215 (85.58%), Query Frame = 0


Query: 1   MASSSANALFSSSLAPSI-----PKSPLLSISNSSVSLNLLPTLHS---SSSASSSRCNF 60
           MASSS+ +LFSS    S       KSPLLS S SSVSLN  P LHS   SSS+SS+RC+F
Sbjct: 1   MASSSSKSLFSSPHFTSFNRSSHSKSPLLSFSTSSVSLNPRP-LHSSITSSSSSSNRCSF 60

Query: 61  IPIRSVLKTVET--FELHTSDLADPTFPNTRSTILVAEKLGEAGLKLLRSFGDVDCAYDL 120
           IP+RSVLKT+E+  F    SDL DPT PN RSTILVAEKLGEAGL+LLRSFG+V CAYDL
Sbjct: 61  IPLRSVLKTLESSAFVSQNSDLVDPTSPNDRSTILVAEKLGEAGLELLRSFGNVVCAYDL 120

Query: 121 SPEQLCAKISSCDALIVRSGTTVNRQLFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLV 180
           SPE+LCAKISSCDALIVRSGT VNRQ+FEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLV
Sbjct: 121 SPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLV 180

Query: 181 VNAPTANTIAAAEHGIALLTAMARNVAQADASMKA 206
           VNAPTANTIAAAEHGIALLTAMARNVAQADAS+KA
Sbjct: 181 VNAPTANTIAAAEHGIALLTAMARNVAQADASIKA 214

[Close]

BLAST of CmoCh01G000270 vs. ExPASy TrEMBL
Match: A0A1S3CKG7 (D-3-phosphoglycerate dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103501977 PE=3 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 2.3e-74
Identity = 171/215 (79.53%), Postives = 184/215 (85.58%), Query Frame = 0


Query: 1   MASSSANALFSSSLAPSI-----PKSPLLSISNSSVSLNLLPTLHS---SSSASSSRCNF 60
           MASSS+ +LFSS    S       KSPLLS S SSVSLN  P LHS   SSS+SS+RC+F
Sbjct: 1   MASSSSKSLFSSPHFTSFNRSSHSKSPLLSFSTSSVSLNPRP-LHSSITSSSSSSNRCSF 60

Query: 61  IPIRSVLKTVET--FELHTSDLADPTFPNTRSTILVAEKLGEAGLKLLRSFGDVDCAYDL 120
           IP+RSVLKT+E+  F    SDL DPT PN RSTILVAEKLGEAGL+LLRSFG+V CAYDL
Sbjct: 61  IPLRSVLKTLESSAFVSQNSDLVDPTSPNDRSTILVAEKLGEAGLELLRSFGNVVCAYDL 120

Query: 121 SPEQLCAKISSCDALIVRSGTTVNRQLFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLV 180
           SPE+LCAKISSCDALIVRSGT VNRQ+FEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLV
Sbjct: 121 SPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLV 180

Query: 181 VNAPTANTIAAAEHGIALLTAMARNVAQADASMKA 206
           VNAPTANTIAAAEHGIALLTAMARNVAQADAS+KA
Sbjct: 181 VNAPTANTIAAAEHGIALLTAMARNVAQADASIKA 214

[Close]

BLAST of CmoCh01G000270 vs. NCBI nr
Match: XP_022949286.1 (D-3-phosphoglycerate dehydrogenase 2, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 377.5 bits (968), Expect = 7.9e-101
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 0


Query: 1   MASSSANALFSSSLAPSIPKSPLLSISNSSVSLNLLPTLHSSSSASSSRCNFIPIRSVLK 60
           MASSSANALFSSSLAPSIPKSPLLSISNSSVSLNLLPTLHSSSSASSSRCNFIPIRSVLK
Sbjct: 1   MASSSANALFSSSLAPSIPKSPLLSISNSSVSLNLLPTLHSSSSASSSRCNFIPIRSVLK 60

Query: 61  TVETFELHTSDLADPTFPNTRSTILVAEKLGEAGLKLLRSFGDVDCAYDLSPEQLCAKIS 120
           TVETFELHTSDLADPTFPNTRSTILVAEKLGEAGLKLLRSFGDVDCAYDLSPEQLCAKIS
Sbjct: 61  TVETFELHTSDLADPTFPNTRSTILVAEKLGEAGLKLLRSFGDVDCAYDLSPEQLCAKIS 120

Query: 121 SCDALIVRSGTTVNRQLFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIA 180
           SCDALIVRSGTTVNRQLFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIA
Sbjct: 121 SCDALIVRSGTTVNRQLFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIA 180

Query: 181 AAEHGIALLTAMARNVAQADASMKA 206
           AAEHGIALLTAMARNVAQADASMKA
Sbjct: 181 AAEHGIALLTAMARNVAQADASMKA 205

[Close]

BLAST of CmoCh01G000270 vs. NCBI nr
Match: KAG6606684.1 (D-3-phosphoglycerate dehydrogenase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036404.1 D-3-phosphoglycerate dehydrogenase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 372.9 bits (956), Expect = 2.0e-99
Identity = 203/205 (99.02%), Postives = 203/205 (99.02%), Query Frame = 0


Query: 1   MASSSANALFSSSLAPSIPKSPLLSISNSSVSLNLLPTLHSSSSASSSRCNFIPIRSVLK 60
           MASSSANALFSSSLAPS PKSPLLSISNSSVSLNLLPTLHSSSSASSSRCNFIPIRSVLK
Sbjct: 1   MASSSANALFSSSLAPSTPKSPLLSISNSSVSLNLLPTLHSSSSASSSRCNFIPIRSVLK 60

Query: 61  TVETFELHTSDLADPTFPNTRSTILVAEKLGEAGLKLLRSFGDVDCAYDLSPEQLCAKIS 120
           TVETFELHTSDLADPTFPNTRSTILVAEKLGEAGLKLLRSFGDV CAYDLSPEQLCAKIS
Sbjct: 61  TVETFELHTSDLADPTFPNTRSTILVAEKLGEAGLKLLRSFGDVHCAYDLSPEQLCAKIS 120

Query: 121 SCDALIVRSGTTVNRQLFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIA 180
           SCDALIVRSGTTVNRQLFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIA
Sbjct: 121 SCDALIVRSGTTVNRQLFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIA 180

Query: 181 AAEHGIALLTAMARNVAQADASMKA 206
           AAEHGIALLTAMARNVAQADASMKA
Sbjct: 181 AAEHGIALLTAMARNVAQADASMKA 205

[Close]

BLAST of CmoCh01G000270 vs. NCBI nr
Match: XP_022998343.1 (D-3-phosphoglycerate dehydrogenase 1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 366.7 bits (940), Expect = 1.4e-97
Identity = 199/205 (97.07%), Postives = 201/205 (98.05%), Query Frame = 0


Query: 1   MASSSANALFSSSLAPSIPKSPLLSISNSSVSLNLLPTLHSSSSASSSRCNFIPIRSVLK 60
           MASSSANALFSSSLAPSIP+SPL SISNSSVSLNLLPTLHSSS  SSSRCNFIPIRSVLK
Sbjct: 1   MASSSANALFSSSLAPSIPESPLFSISNSSVSLNLLPTLHSSSFISSSRCNFIPIRSVLK 60

Query: 61  TVETFELHTSDLADPTFPNTRSTILVAEKLGEAGLKLLRSFGDVDCAYDLSPEQLCAKIS 120
           TVET ELHTSDLADPTFPNTRSTILVAEKLGEAGLKLLRSFGDVDCAYDLSPEQLCAKIS
Sbjct: 61  TVETLELHTSDLADPTFPNTRSTILVAEKLGEAGLKLLRSFGDVDCAYDLSPEQLCAKIS 120

Query: 121 SCDALIVRSGTTVNRQLFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIA 180
           SCDALIVRSGTTVNRQ+FEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIA
Sbjct: 121 SCDALIVRSGTTVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIA 180

Query: 181 AAEHGIALLTAMARNVAQADASMKA 206
           AAEHGIALLTAMARNVAQADASMKA
Sbjct: 181 AAEHGIALLTAMARNVAQADASMKA 205

[Close]

BLAST of CmoCh01G000270 vs. NCBI nr
Match: XP_023525881.1 (D-3-phosphoglycerate dehydrogenase 2, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 358.6 bits (919), Expect = 3.8e-95
Identity = 198/205 (96.59%), Postives = 199/205 (97.07%), Query Frame = 0


Query: 1   MASSSANALFSSSLAPSIPKSPLLSISNSSVSLNLLPTLHSSSSASSSRCNFIPIRSVLK 60
           MASSSANALFSSSLAPSIPKSPLLSISNSSVSLNLLPTL SS   SSSRCNFIPIRSVLK
Sbjct: 1   MASSSANALFSSSLAPSIPKSPLLSISNSSVSLNLLPTLPSS---SSSRCNFIPIRSVLK 60

Query: 61  TVETFELHTSDLADPTFPNTRSTILVAEKLGEAGLKLLRSFGDVDCAYDLSPEQLCAKIS 120
           TVET ELHTSDLADPTFPNTRSTILVAEKLGEAGLKLLRSFGDVDCAYDLSPE LCAKIS
Sbjct: 61  TVETLELHTSDLADPTFPNTRSTILVAEKLGEAGLKLLRSFGDVDCAYDLSPEHLCAKIS 120

Query: 121 SCDALIVRSGTTVNRQLFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIA 180
           SCDALIVRSGTTVNRQ+FEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIA
Sbjct: 121 SCDALIVRSGTTVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIA 180

Query: 181 AAEHGIALLTAMARNVAQADASMKA 206
           AAEHGIALLTAMARNVAQADASMKA
Sbjct: 181 AAEHGIALLTAMARNVAQADASMKA 202

[Close]

BLAST of CmoCh01G000270 vs. NCBI nr
Match: XP_038895985.1 (D-3-phosphoglycerate dehydrogenase 1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 303.5 bits (776), Expect = 1.5e-78
Identity = 178/215 (82.79%), Postives = 187/215 (86.98%), Query Frame = 0


Query: 1   MASSSANALFSSSLAPSI-----PKSPLLSISNSSVSLNLLPTLHS---SSSASSSRCNF 60
           MASSSA +LFSS    S       KSPLLS SNSSVSLNL P LHS   SSS+SS+RCNF
Sbjct: 1   MASSSAKSLFSSPHFTSFNRSSHSKSPLLSFSNSSVSLNLHP-LHSSSPSSSSSSNRCNF 60

Query: 61  IPIRSVLKTVET--FELHTSDLADPTFPNTRSTILVAEKLGEAGLKLLRSFGDVDCAYDL 120
           IP+RSVLKTVE+   E+  SDL  PTFPN RSTILVAEKLGEAGLKLLRSFG+V+CAYDL
Sbjct: 61  IPLRSVLKTVESSGLEVQNSDLVGPTFPNDRSTILVAEKLGEAGLKLLRSFGNVECAYDL 120

Query: 121 SPEQLCAKISSCDALIVRSGTTVNRQLFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLV 180
           SPE LCAKISSCDALIVRSGT VNRQ+FEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLV
Sbjct: 121 SPEDLCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLV 180

Query: 181 VNAPTANTIAAAEHGIALLTAMARNVAQADASMKA 206
           VNAPTANTIAAAEHGIALLTAMARNVAQADASMKA
Sbjct: 181 VNAPTANTIAAAEHGIALLTAMARNVAQADASMKA 214

[Close]

BLAST of CmoCh01G000270 vs. TAIR 10
Match: AT1G17745.1 (D-3-phosphoglycerate dehydrogenase )

HSP 1 Score: 203.0 bits (515), Expect = 2.5e-52
Identity = 114/161 (70.81%), Postives = 125/161 (77.64%), Query Frame = 0


Query: 55  IRSVLKTVETFEL----------HTSDLADPTFPNTRSTILVAEKLGEAGLKLLRSFGDV 114
           I + LKTVE   L            SD  +PT P  R  ILV EKLGEAG+ LLR FGDV
Sbjct: 48  ISAALKTVEQTTLTEDNRFSTVGSDSDEYNPTLPKPR--ILVTEKLGEAGVNLLREFGDV 107

Query: 115 DCAYDLSPEQLCAKISSCDALIVRSGTTVNRQLFEAAKGRLKVVGRAGVGIDNVDLQAAT 174
           DC+YDLSPE L  K++  DALIVRSGT V R++FEAAKGRLKVVGRAGVGIDNVDLQAAT
Sbjct: 108 DCSYDLSPEDLKKKVAESDALIVRSGTKVTREVFEAAKGRLKVVGRAGVGIDNVDLQAAT 167

Query: 175 EFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKA 206
           E GCLVVNAPTANT+AAAEHGIALL +MARNVAQADAS+KA
Sbjct: 168 EHGCLVVNAPTANTVAAAEHGIALLASMARNVAQADASIKA 206

[Close]

BLAST of CmoCh01G000270 vs. TAIR 10
Match: AT1G17745.2 (D-3-phosphoglycerate dehydrogenase )

HSP 1 Score: 203.0 bits (515), Expect = 2.5e-52
Identity = 114/161 (70.81%), Postives = 125/161 (77.64%), Query Frame = 0


Query: 55  IRSVLKTVETFEL----------HTSDLADPTFPNTRSTILVAEKLGEAGLKLLRSFGDV 114
           I + LKTVE   L            SD  +PT P  R  ILV EKLGEAG+ LLR FGDV
Sbjct: 48  ISAALKTVEQTTLTEDNRFSTVGSDSDEYNPTLPKPR--ILVTEKLGEAGVNLLREFGDV 107

Query: 115 DCAYDLSPEQLCAKISSCDALIVRSGTTVNRQLFEAAKGRLKVVGRAGVGIDNVDLQAAT 174
           DC+YDLSPE L  K++  DALIVRSGT V R++FEAAKGRLKVVGRAGVGIDNVDLQAAT
Sbjct: 108 DCSYDLSPEDLKKKVAESDALIVRSGTKVTREVFEAAKGRLKVVGRAGVGIDNVDLQAAT 167

Query: 175 EFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKA 206
           E GCLVVNAPTANT+AAAEHGIALL +MARNVAQADAS+KA
Sbjct: 168 EHGCLVVNAPTANTVAAAEHGIALLASMARNVAQADASIKA 206

[Close]

BLAST of CmoCh01G000270 vs. TAIR 10
Match: AT3G19480.1 (D-3-phosphoglycerate dehydrogenase )

HSP 1 Score: 194.5 bits (493), Expect = 8.9e-50
Identity = 110/176 (62.50%), Postives = 133/176 (75.57%), Query Frame = 0


Query: 30  SVSLNLLPTLHSSSSASSSRCNFIPIRSVLKTVETFELHTSDLADPTFPNTRSTILVAEK 89
           + SLNL     SSS   ++  +  PIR   + +    L TS  +       + TILV EK
Sbjct: 2   ATSLNLSSIFSSSSRLVTTPSSVFPIRQRRRII----LVTSSSSG---GGGKPTILVTEK 61

Query: 90  LGEAGLKLLRSFGDVDCAYDLSPEQLCAKISSCDALIVRSGTTVNRQLFEAAKGRLKVVG 149
           LG+AG+ LL+ + +VDC+YDLS E+LC KIS CDALIVRSGT V R +FE+++GRLKVVG
Sbjct: 62  LGQAGIDLLKKYANVDCSYDLSLEELCTKISLCDALIVRSGTKVGRDVFESSRGRLKVVG 121

Query: 150 RAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKA 206
           RAGVGIDNVDL AATE+GCLVVNAPTANT+AAAEHGIALLTAMARN+AQADAS+KA
Sbjct: 122 RAGVGIDNVDLAAATEYGCLVVNAPTANTVAAAEHGIALLTAMARNIAQADASIKA 170

[Close]

BLAST of CmoCh01G000270 vs. TAIR 10
Match: AT4G34200.1 (D-3-phosphoglycerate dehydrogenase )

HSP 1 Score: 191.0 bits (484), Expect = 9.8e-49
Identity = 99/132 (75.00%), Postives = 115/132 (87.12%), Query Frame = 0


Query: 76  TFPNTRSTILVAEKLGEAGLKLLRSFGDVDCAYDLSPEQLCAKISSCDALIVRSGTTVNR 135
           T   ++ TILVAEKLG+AG+KLL    +VDC+Y+++PE+L  KIS CDALIVRSGT V R
Sbjct: 56  TGDGSKPTILVAEKLGDAGIKLLEDVANVDCSYNMTPEELNIKISLCDALIVRSGTKVGR 115

Query: 136 QLFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTAMARN 195
           ++FE++ GRLKVVGRAGVGIDNVDL AATEFGCLVVNAPTANTIAAAEHGIAL+ AMARN
Sbjct: 116 EVFESSHGRLKVVGRAGVGIDNVDLSAATEFGCLVVNAPTANTIAAAEHGIALMAAMARN 175

Query: 196 VAQADASMKAVE 208
           VAQADAS+KA E
Sbjct: 176 VAQADASVKAGE 187

The following BLAST results are available for this feature:

BLAST of CmoCh01G000270 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04130	3.5e-51	70.81	D-3-phosphoglycerate dehydrogenase 2, chloroplastic OS=Arabidopsis thaliana OX=3...	[more]
Q9LT69	1.2e-48	62.50	D-3-phosphoglycerate dehydrogenase 3, chloroplastic OS=Arabidopsis thaliana OX=3...	[more]
O49485	1.4e-47	75.00	D-3-phosphoglycerate dehydrogenase 1, chloroplastic OS=Arabidopsis thaliana OX=3...	[more]
Q58424	1.3e-24	50.81	D-3-phosphoglycerate dehydrogenase OS=Methanocaldococcus jannaschii (strain ATCC...	[more]
O27051	1.5e-22	44.80	D-3-phosphoglycerate dehydrogenase OS=Methanothermobacter thermautotrophicus (st...	[more]

BLAST of CmoCh01G000270 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GBM1	3.8e-101	100.00	D-3-phosphoglycerate dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111452687...	[more]
A0A6J1KGG7	6.8e-98	97.07	D-3-phosphoglycerate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111493010 P...	[more]
A0A0A0LC35	6.2e-75	80.00	D-3-phosphoglycerate dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_3G651740 PE...	[more]
A0A5A7VDA4	2.3e-74	79.53	D-3-phosphoglycerate dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C...	[more]
A0A1S3CKG7	2.3e-74	79.53	D-3-phosphoglycerate dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103501977 PE=3 ...	[more]

BLAST of CmoCh01G000270 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022949286.1	7.9e-101	100.00	D-3-phosphoglycerate dehydrogenase 2, chloroplastic-like [Cucurbita moschata]	[more]
KAG6606684.1	2.0e-99	99.02	D-3-phosphoglycerate dehydrogenase 2, chloroplastic, partial [Cucurbita argyrosp...	[more]
XP_022998343.1	1.4e-97	97.07	D-3-phosphoglycerate dehydrogenase 1, chloroplastic-like [Cucurbita maxima]	[more]
XP_023525881.1	3.8e-95	96.59	D-3-phosphoglycerate dehydrogenase 2, chloroplastic-like [Cucurbita pepo subsp. ...	[more]
XP_038895985.1	1.5e-78	82.79	D-3-phosphoglycerate dehydrogenase 1, chloroplastic-like [Benincasa hispida]	[more]

BLAST of CmoCh01G000270 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G17745.1	2.5e-52	70.81	D-3-phosphoglycerate dehydrogenase	[more]
AT1G17745.2	2.5e-52	70.81	D-3-phosphoglycerate dehydrogenase	[more]
AT3G19480.1	8.9e-50	62.50	D-3-phosphoglycerate dehydrogenase	[more]
AT4G34200.1	9.8e-49	75.00	D-3-phosphoglycerate dehydrogenase	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.720		coord: 81..215 e-value: 1.1E-34 score: 120.9
None	No IPR available	PANTHER	PTHR42938:SF22	D-3-PHOSPHOGLYCERATE DEHYDROGENASE 2, CHLOROPLASTIC	coord: 47..207
None	No IPR available	PANTHER	PTHR42938	FORMATE DEHYDROGENASE 1	coord: 47..207
None	No IPR available	SUPERFAMILY	52283	Formate/glycerate dehydrogenase catalytic domain-like	coord: 83..205
IPR006139	D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain	PFAM	PF00389	2-Hacid_dh	coord: 84..209 e-value: 1.0E-28 score: 99.6

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh01G000270.1	CmoCh01G000270.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006564	L-serine biosynthetic process
cellular_component	GO:0009570	chloroplast stroma
molecular_function	GO:0051287	NAD binding
molecular_function	GO:0004617	phosphoglycerate dehydrogenase activity
molecular_function	GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor