CmoCh01G000140 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh01G000140
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	(S)-2-hydroxy-acid oxidase
Location	Cmo_Chr01: 46814 .. 51364 (-)
RNA-Seq Expression	CmoCh01G000140
Synteny	CmoCh01G000140

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTAGTAAGTAATATTAGAACATGCAAAGAGGATGTACCCGAAAAGGGTTTTTTTTTCTCTTTTTTTTTTCTGTAAATACTAAGCTTGCCTTTTCTCATTTGTATTTGGATATTTTCATTATATATATATATATATCGAGAGAGAGAGAGAGAGAGAGAGAGAGCTCTAATCCTATCTTCAATCTTGGCACATCTGTGGAAATTAAAGCAACAGAATTGATTGATAAATAATAATGGGTCGTAATGTTTGGGCTGGGTGTGTTATACGTATGGCCCACTTGGTGGATGTTAAATGTATTTTCAGGAGTGTGTGTTCCATTTGAGATTTGAATTAACCATAATTAAAAAATTGTTACAATCATATGATCTTTTGATATATGAATTAAGGTCAAATCTATCTCCAATTGAATTGAATGCTCAAAAAAGAAATCAAATTGAAAATTGAAATTCAATTATCTCCTCAAATAACCCTGCAAAGGGCCGGAGTTGTCAACGTTAGAATAGACGACACAGCACTGATTGGCGTTAATTACGATAAAGCAGAGTAGTTGGAATGTATGAACAAATTTGCAATCTCCATCGGTTTTAGGTTGGTATCTATACATATATATATTTGTTCTACACTTCCACTTCCAGCAGCCACGTAATCCTTATCAACACTTTTATTTTCGGCTTCTACTTCAACTTCTCCACCTTTTCTCCGGTGAAGGCTGCAGTTTCGAGTTTAGGGTTAACAAACCTCAAGGTATAGATCCTGTTTTTAATTTCTCCATAAATATGTCACCAACTCTTGTTCCTTACTTTCAACCTTCTGTTCATCCCAAAATCTCTCATCATAAATTTAACCAAGTACAGAAAGTTCTTTTAATTCATTCCCGCGTCTTTCAACACCTTCATGTTTAATCCATCCGTGGGGGACATCTGTTACTGTTTTTCTGGTCACTTTTGTTCCTTACTTTCAACGTCCTGTTCATACCAAAATCTCTTATCATAAATTTAACAAAGTACAGAAAATTCTTTTAACTCATTCCCGCGTCTTTCAACACCTCTATGTTTAATCGATCCGTGGGCGACATCTGTTACTGTTTCTCTGTCAAATTTGAATATAATCACTTAATTAGGAACCTTCTGATCTCTATTTTGTCAGGGTAAAATGTCTTCTAAACCAGTGAATGTGAATGACTTCAAGGAATTGGCTAGATCAGCTCTTCCCAAAATGTATTACGATTTCTATGCAGGGGGAGCTGAGGACGAGCACACCCTAAGAGAGAACATACGGGCCTTTCATAGCATCACGTAATTTTAAACCTGCCTAATCACTCTTTTGATAGAGTAATGCTATATACTTACTATTCGGGGTTTCTATGGTTTTTAGGATTCGACCTCGAGTTCTTTTGGATGTGAGCCAAATTGATATGTCAACAACTGTATTAGGCCATCGCATCTCTGCACCTATCCTGGTTGCTCCCACTTCTGCGCATATGTTGGCATTCCATGAAGGTTGTTGAGAACATCGCTTAGTTGAGAGAACTGATTTTCATTCTACTTATACCTGATGATATCTATGTTTTTTCAGGAGAACTTGCCACGGCTAGGGCAGCAGCTGCTGCACAAGCTATAAGTTATTTAACCTGTGCAATTTGGTAATTGAATTGAGTTGCTTCCGTAGGTGCTGTCCTATTCTTCAACCCGTTCAATAGAGGAAGTGGCAGCTAGCTGGGACGCTGTCCGCTTCTTTCAATTATATGTAAACCACTCTTCTTTTTCACGTTTAACTATCTTTCCTTGTTGGCAAATTTGAATTTATCCTCCATTTTGTAATCAGGTTTTCAAAAGGCGTGATATCACAGCTTTGATAGTACAGAGAGCTGAGACATGTGGATACAAGGCCATTGTGGTGACTCTTGATACTCCTTGGCTTGGTCGAAGGGAGGCTGACATGAAGAACAATTATCTATGCTTCACATCTCATGCATTGTATGTATTCCTTTTGCTGGGTATTGAGTGCTTCAATTGTAAGCTACGCCTTTGTCTTTGTCTCATCTGGAAAATCAATTGGATGAATTTGTAGGATGATTGCATATCCAAAGAAGAATCTTGAAGGCCTCATGTCTGTTGAAATTAACTCTGTGAGTAATGGTTCAATTTTCTTGTTTAGAATATTGGAATTACATCTCACGATTAAATCTAGATATATTTGTTAGGAATCACGATTCTCCAAAATAGTATTGTATTGTCCACTTTAAACACAATCTCTCATGGCTTTGCTTTGGGCTTCTCCAAAAGGCCTCGTACTAATGGAGATGTATTCCTTGCTTATAAATCCATGGTGATTCCCTAAATTAGCCAATGTGGGACTCCTTCCCAACCATCCTCATCACTATTTTCATGCAACTTTTGCGAACCCGGTGTACAATTATGAACTTTTGCGAAAGCAACTGTGGACGCATCTTTATCCTGGAAAGTAAGTACAGAAAGCTGCTCAGATTCCTATCACACACTTGAATGTGTGCTTTACTTTGGTGCTTAATAAGCTTTGGAGCATATACTTCATATAAATCAGTAATTTAACTACTCCTATGTTGTATGGACTTCTTTCAACTACGGTAATGTAATGAATATACAGTGTTTGAGAAATCTTTCGAATCATCATCTTGATTAGATGAGATTTTCTGCTACTTGAAAGGTTTATTCTTCTAACATTCATGTCCTTCCAGTTCAGTTTTAATATTTTTCTGTTTACAGGGTATAAAGTGGTTAAGATCAATCACTAGTCTGCCAATTCTGTTAAGGGGGATTCTCACTCGTGAAGATGGTAAGTTAAGTTATGTTTTTAAGTGTTAGGAGAAAACTAATAAATACTACGCCCAAGCGATAAACACAAGTAGCAATTGTCAAGTACGTATCAGATATGTAATGGCCCAAGCCTACCGCTAATAGATATTGTCTTCTTTGGGCTTTCTCTCAAGGCTTTAATACGCGTTTGCTAGGAAAAGGTTTCTACACCCTTATAATGGGTGTTTCGTTCTCCTCCCCATCCAATGTGGGATATTACAATCCACCCCCTTTAGGGCCCAGCGCCATCGCTGACACTCGTTCCTTTCTCCAATCGATGTGGGACCCCACCAAATCACCCCCCTTCGGGACCCAGCGTCCTTACTGGCACACCACCTCGTGTCTACTTCATTCGGAGAATAGCCTCCTCGCTGGCACATCGCCCGGTGTGATACGATGAATGAAGTAAAAGATGTGATGACTTGTAGAAATGCTTATCCATCTCAGACAGCACAATTTTCCTTTCAATATATTTCCTTGCTTGGTTTATGAGTTTTGTCTGAGTTCATGGATAGGCAATTTCTTTCATTAGCTTTCTTGATGATTGGCAGCAACTAAAGCTGTGGAAGCTGGTGTTGATGGAATTATTGTCTCCAATCATGGAGGCCGCCAACTAGACTTTACTCCGCCCACTATTCTCGTTCTCGAAGAGGTCAGTTTCGCTTCATTTTGTGCTACTATATTGCTCAAAGATGTGAATGCAAGGAGATAAAATTACTTCAATTTCTGCATAAGTTAGCTTCACATTACTTCGACATGTGTCTCTGCAAATATTGTCCTTCATTTTTAAGCTTTTAAATCAAATCTTTGTTTTGAAATAGACGAGTCTTTAGTTTTAGTAAATGGTCCCTCCCAATTCACTCCATGTTCTCGTGAGAATTCTACATTCGTTGGGGACACAATCTCACATTAGTTGGGAGGAGAACGAAACACCTTTATAAGGGTGTGGAAACCTTTCCCTAACAGACACATTTTAAAGCTTTGAGGAAAATCCCGTAAGAAAAAGTTCAAGGAGGACAATATATACTAGAGGTAGCCTGAACTGTTACGGTTGTGCAGGGTCAAAACCAAAACCAAGTTGATACAAATTTAGGACAAATATGAGTGAACCCAAATGCATATTCACGGCTTCAATTTGCGCAGCCTTTTGGGTTTGTGTCCTAATTGATAAGCATGAAGTTTGATGCAAGTTGTCCAGGCTGTGAAAGAAGTTCCAGTGGTCCTAGATGGAGGTGTGCGGCGAGGAACAGATGTATTTAAGGGATTAGCCCTTGGGGCACAGGCAATCCTTGTAAGTTCTATACTCCCCCCATATAAAACATTTAAAATGGTACTATTATTATTATTTTTGTGTGCGATGGTATAATTTGATGGTGATTCAGACGGGGAGAGCAGTTTTATTTGGGCCATCTGCAAAGGGAGAAGGAGCAAGAACAGTGCTGGAAATGCTGAAGAATGAGTTGGAGACCACCATGGCACTCTCAGGTTGCCCATCTATTAGGGATATTACTAGAAACCATGTTAGGACACAGTATGATAAGCTGCAAGCAATGCTTTGACGTGTTTGCCTTTTATTATGTAACCAAGCACAGCTACTCTACTGTTTCACAAGAAGACAGGTCTCGGCAACGATGCATCTCTCTAAATTCAGTAATGTTATTACATTATTTGGCAACTTCTCAACGAAACAAAAAATCTACCCTTGGATCATCTAT

mRNA sequence

ATGTACCACGTAATCCTTATCAACACTTTTATTTTCGGCTTCTACTTCAACTTCTCCACCTTTTCTCCGGTGAAGGCTGCAGTTTCGAGTTTAGGGTTAACAAACCTCAAGGGTAAAATGTCTTCTAAACCAGTGAATGTGAATGACTTCAAGGAATTGGCTAGATCAGCTCTTCCCAAAATGTATTACGATTTCTATGCAGGGGGAGCTGAGGACGAGCACACCCTAAGAGAGAACATACGGGCCTTTCATAGCATCACGATTCGACCTCGAGTTCTTTTGGATGTGAGCCAAATTGATATGTCAACAACTGTATTAGGCCATCGCATCTCTGCACCTATCCTGGTTGCTCCCACTTCTGCGCATATGTTGGCATTCCATGAAGGAGAACTTGCCACGGCTAGGGCAGCAGCTGCTGCACAAGCTATAAAGGAAGTGGCAGCTAGCTGGGACGCTGTCCGCTTCTTTCAATTATATGTTTTCAAAAGGCGTGATATCACAGCTTTGATAGTACAGAGAGCTGAGACATGTGGATACAAGGCCATTGTGGTGACTCTTGATACTCCTTGGCTTGGTCGAAGGGAGGCTGACATGAAGAACAATTATCTATGCTTCACATCTCATGCATTGATGATTGCATATCCAAAGAAGAATCTTGAAGGCCTCATGTCTGTTGAAATTAACTCTGGTATAAAGTGGTTAAGATCAATCACTAGTCTGCCAATTCTGTTAAGGGGGATTCTCACTCGTGAAGATGCAACTAAAGCTGTGGAAGCTGGTGTTGATGGAATTATTGTCTCCAATCATGGAGGCCGCCAACTAGACTTTACTCCGCCCACTATTCTCGTTCTCGAAGAGGCTGTGAAAGAAGTTCCAGTGGTCCTAGATGGAGGTGTGCGGCGAGGAACAGATGTATTTAAGGGATTAGCCCTTGGGGCACAGGCAATCCTTACGGGGAGAGCAGTTTTATTTGGGCCATCTGCAAAGGGAGAAGGAGCAAGAACAGTGCTGGAAATGCTGAAGAATGAGTTGGAGACCACCATGGCACTCTCAGGTTGCCCATCTATTAGGGATATTACTAGAAACCATGTTAGGACACAGTATGATAAGCTGCAAGCAATGCTTTGACGTGTTTGCCTTTTATTATGTAACCAAGCACAGCTACTCTACTGTTTCACAAGAAGACAGGTCTCGGCAACGATGCATCTCTCTAAATTCAGTAATGTTATTACATTATTTGGCAACTTCTCAACGAAACAAAAAATCTACCCTTGGATCATCTAT

Coding sequence (CDS)

ATGTACCACGTAATCCTTATCAACACTTTTATTTTCGGCTTCTACTTCAACTTCTCCACCTTTTCTCCGGTGAAGGCTGCAGTTTCGAGTTTAGGGTTAACAAACCTCAAGGGTAAAATGTCTTCTAAACCAGTGAATGTGAATGACTTCAAGGAATTGGCTAGATCAGCTCTTCCCAAAATGTATTACGATTTCTATGCAGGGGGAGCTGAGGACGAGCACACCCTAAGAGAGAACATACGGGCCTTTCATAGCATCACGATTCGACCTCGAGTTCTTTTGGATGTGAGCCAAATTGATATGTCAACAACTGTATTAGGCCATCGCATCTCTGCACCTATCCTGGTTGCTCCCACTTCTGCGCATATGTTGGCATTCCATGAAGGAGAACTTGCCACGGCTAGGGCAGCAGCTGCTGCACAAGCTATAAAGGAAGTGGCAGCTAGCTGGGACGCTGTCCGCTTCTTTCAATTATATGTTTTCAAAAGGCGTGATATCACAGCTTTGATAGTACAGAGAGCTGAGACATGTGGATACAAGGCCATTGTGGTGACTCTTGATACTCCTTGGCTTGGTCGAAGGGAGGCTGACATGAAGAACAATTATCTATGCTTCACATCTCATGCATTGATGATTGCATATCCAAAGAAGAATCTTGAAGGCCTCATGTCTGTTGAAATTAACTCTGGTATAAAGTGGTTAAGATCAATCACTAGTCTGCCAATTCTGTTAAGGGGGATTCTCACTCGTGAAGATGCAACTAAAGCTGTGGAAGCTGGTGTTGATGGAATTATTGTCTCCAATCATGGAGGCCGCCAACTAGACTTTACTCCGCCCACTATTCTCGTTCTCGAAGAGGCTGTGAAAGAAGTTCCAGTGGTCCTAGATGGAGGTGTGCGGCGAGGAACAGATGTATTTAAGGGATTAGCCCTTGGGGCACAGGCAATCCTTACGGGGAGAGCAGTTTTATTTGGGCCATCTGCAAAGGGAGAAGGAGCAAGAACAGTGCTGGAAATGCTGAAGAATGAGTTGGAGACCACCATGGCACTCTCAGGTTGCCCATCTATTAGGGATATTACTAGAAACCATGTTAGGACACAGTATGATAAGCTGCAAGCAATGCTTTGA

Protein sequence

MYHVILINTFIFGFYFNFSTFSPVKAAVSSLGLTNLKGKMSSKPVNVNDFKELARSALPKMYYDFYAGGAEDEHTLRENIRAFHSITIRPRVLLDVSQIDMSTTVLGHRISAPILVAPTSAHMLAFHEGELATARAAAAAQAIKEVAASWDAVRFFQLYVFKRRDITALIVQRAETCGYKAIVVTLDTPWLGRREADMKNNYLCFTSHALMIAYPKKNLEGLMSVEINSGIKWLRSITSLPILLRGILTREDATKAVEAGVDGIIVSNHGGRQLDFTPPTILVLEEAVKEVPVVLDGGVRRGTDVFKGLALGAQAILTGRAVLFGPSAKGEGARTVLEMLKNELETTMALSGCPSIRDITRNHVRTQYDKLQAML

Homology

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BLAST of CmoCh01G000140 vs. ExPASy Swiss-Prot
Match: Q24JJ8 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO3 OS=Arabidopsis thaliana OX=3702 GN=GLO3 PE=2 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 3.2e-113
Identity = 221/368 (60.05%), Postives = 270/368 (73.37%), Query Frame = 0


Query: 45  VNVNDFKELARSALPKMYYDFYAGGAEDEHTLRENIRAFHSITIRPRVLLDVSQIDMSTT 104
           VNV++F+ELA+ ALPKMYYDFY GGAED+HTL EN++AF  I  RPRVL+DVS+IDMST 
Sbjct: 5   VNVDEFQELAKQALPKMYYDFYNGGAEDQHTLNENVQAFRRIMFRPRVLVDVSKIDMSTK 64

Query: 105 VLGHRISAPILVAPTSAHMLAFHEGELATARAAAAAQAI-----------KEVAASWDAV 164
           +LG+ ISAPI++APT  H LA  EGE ATA+AAAA   I           +E+A+S +AV
Sbjct: 65  ILGYPISAPIMIAPTGNHKLAHPEGETATAKAAAACNTIMIVSYMSSCTFEEIASSCNAV 124

Query: 165 RFFQLYVFKRRDITALIVQRAETCGYKAIVVTLDTPWLGRREADMKNNYLCFTSHALMIA 224
           RF Q+YV+KRRDITA +V+RAE  G+KAIV+T+D P LGRREAD+KN          MI+
Sbjct: 125 RFLQIYVYKRRDITAQVVKRAEKAGFKAIVLTVDVPRLGRREADIKNK---------MIS 184

Query: 225 YPKKNLEGLMSVEIN---------------------SGIKWLRSITSLPILLRGILTRED 284
              KN EGL S E+                        I+WLRSIT LPIL++GILTRED
Sbjct: 185 PQLKNFEGLFSTEVRPSKGSGVQAFASRAFDASFSWKDIEWLRSITELPILVKGILTRED 244

Query: 285 ATKAVEAGVDGIIVSNHGGRQLDFTPPTILVLEEAVK----EVPVVLDGGVRRGTDVFKG 344
           A KAVEAGVDGIIVSNHGGRQLD++P TI VLEE V+     +PV+LDGGVRRGTDVFK 
Sbjct: 245 ALKAVEAGVDGIIVSNHGGRQLDYSPATITVLEEVVQVVRGRIPVLLDGGVRRGTDVFKA 304

Query: 345 LALGAQAILTGRAVLFGPSAKGE-GARTVLEMLKNELETTMALSGCPSIRDITRNHVRTQ 376
           LALGAQA+L GR +++G +AKGE G + V++MLKNE E TMALSGCP+I DITRNHVRT+
Sbjct: 305 LALGAQAVLIGRPIIYGLAAKGEDGVKKVIDMLKNEFEITMALSGCPTIDDITRNHVRTE 363

[Close]

BLAST of CmoCh01G000140 vs. ExPASy Swiss-Prot
Match: Q9LJH5 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Arabidopsis thaliana OX=3702 GN=GLO4 PE=2 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 4.7e-112
Identity = 218/368 (59.24%), Postives = 274/368 (74.46%), Query Frame = 0


Query: 45  VNVNDFKELARSALPKMYYDFYAGGAEDEHTLRENIRAFHSITIRPRVLLDVSQIDMSTT 104
           VNV++F+ELA+ ALPKMYYDFY GGAED+HTL EN++AF  I  RPRVL+DVS IDMST+
Sbjct: 5   VNVDEFQELAKQALPKMYYDFYNGGAEDQHTLNENVQAFRRIMFRPRVLVDVSNIDMSTS 64

Query: 105 VLGHRISAPILVAPTSAHMLAFHEGELATARAAAAAQ-----------AIKEVAASWDAV 164
           +LG+ ISAPI++APT+ H LA  +GE+ATA+AAAA              I+EVA+S +AV
Sbjct: 65  MLGYPISAPIMIAPTAMHKLAHPKGEIATAKAAAACNTIMIVSFMSTCTIEEVASSCNAV 124

Query: 165 RFFQLYVFKRRDITALIVQRAETCGYKAIVVTLDTPWLGRREADMKNNYLCFTSHALMIA 224
           RF Q+YV+KRRD+TA IV+RAE  G+KAIV+T+D P LGRREAD+KN          MI+
Sbjct: 125 RFLQIYVYKRRDVTAQIVKRAEKAGFKAIVLTVDVPRLGRREADIKNK---------MIS 184

Query: 225 YPKKNLEGLMSVEI--NSG-------------------IKWLRSITSLPILLRGILTRED 284
              KN EGL+S E+  N G                   I+WLRSIT LPIL++G+LTRED
Sbjct: 185 PQLKNFEGLVSTEVRPNEGSGVEAFASSAFDASLSWKDIEWLRSITKLPILVKGLLTRED 244

Query: 285 ATKAVEAGVDGIIVSNHGGRQLDFTPPTILVLEEAVK----EVPVVLDGGVRRGTDVFKG 344
           A KAVEAGVDGI+VSNHG RQLD++P TI VLEE V      +PV+LDGGVRRGTDVFK 
Sbjct: 245 ALKAVEAGVDGIVVSNHGARQLDYSPATITVLEEVVHAVKGRIPVLLDGGVRRGTDVFKA 304

Query: 345 LALGAQAILTGRAVLFGPSAKGE-GARTVLEMLKNELETTMALSGCPSIRDITRNHVRTQ 376
           LALGAQA+L GR +++G +AKGE G + V++MLKNE E TMALSGCP+I D+TRNHVRT+
Sbjct: 305 LALGAQAVLIGRPIVYGLAAKGEDGVKKVIDMLKNEFEITMALSGCPTIDDVTRNHVRTE 363

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BLAST of CmoCh01G000140 vs. ExPASy Swiss-Prot
Match: Q8H3I4 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Oryza sativa subsp. japonica OX=39947 GN=GLO4 PE=1 SV=2)

HSP 1 Score: 371.3 bits (952), Expect = 1.3e-101
Identity = 198/371 (53.37%), Postives = 266/371 (71.70%), Query Frame = 0


Query: 44  PVNVNDFKELARSALPKMYYDFYAGGAEDEHTLRENIRAFHSITIRPRVLLDVSQIDMST 103
           PVNV +++ELA+ ALPKM YD+  GGAEDEHTLRENI A+  I +RPRVL+DVS+IDMST
Sbjct: 6   PVNVREYQELAKKALPKMAYDYINGGAEDEHTLRENIAAYTRIILRPRVLVDVSKIDMST 65

Query: 104 TVLGHRISAPILVAPTSAHMLAFHEGELATARAAAAAQA-----------IKEVAASWDA 163
           T+LG+ + +PI+VAPT  H LA  EGE ATARAAA+  A           I++VA+S +A
Sbjct: 66  TLLGYTMRSPIIVAPTGGHKLAHPEGEKATARAAASCNAIMVLSFSSSCKIEDVASSCNA 125

Query: 164 VRFFQLYVFKRRDITALIVQRAETCGYKAIVVTLDTPWLGRREADMKNNYLCFTSHALMI 223
           +RF+QLYV+K R+++A +V+RAE+CG+KA+++T+DTP LGRREAD++N           +
Sbjct: 126 IRFYQLYVYKNRNVSATLVRRAESCGFKALLLTVDTPMLGRREADIRNK----------M 185

Query: 224 AYPKK-NLEGLMSVEIN----------------------SGIKWLRSITSLPILLRGILT 283
            +P+  NLEGLM+ + +                        I+WL+SITS+PI L+GI+T
Sbjct: 186 VFPRSGNLEGLMTTDDHDTTNGSQLERFARATLDPSLSWKDIEWLKSITSMPIFLKGIVT 245

Query: 284 REDATKAVEAGVDGIIVSNHGGRQLDFTPPTILVLEEAVK----EVPVVLDGGVRRGTDV 343
            EDA +AVEAGV G+IVSNHG RQLD+ P TI  LEE V+     VPV++DGG+RRGTDV
Sbjct: 246 AEDARRAVEAGVAGVIVSNHGARQLDYAPATIAALEEVVRAVAGAVPVLVDGGIRRGTDV 305

Query: 344 FKGLALGAQAILTGRAVLFGPSAKGE-GARTVLEMLKNELETTMALSGCPSIRDITRNHV 376
           FK LALGA+A++ GR V FG +A+GE GAR V+EML  ELE  MAL GC S+ +ITR+HV
Sbjct: 306 FKALALGARAVMVGRPVFFGLAARGEAGARHVIEMLNGELEVAMALCGCRSVGEITRSHV 365

[Close]

BLAST of CmoCh01G000140 vs. ExPASy Swiss-Prot
Match: B8B8K5 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Oryza sativa subsp. indica OX=39946 GN=GLO4 PE=3 SV=2)

HSP 1 Score: 367.1 bits (941), Expect = 2.4e-100
Identity = 196/371 (52.83%), Postives = 265/371 (71.43%), Query Frame = 0


Query: 44  PVNVNDFKELARSALPKMYYDFYAGGAEDEHTLRENIRAFHSITIRPRVLLDVSQIDMST 103
           PVNV +++ELA+ ALPKM YD+  GGAEDEHTLRENI A+  I +RPRVL+DVS+IDMST
Sbjct: 6   PVNVREYQELAKKALPKMAYDYINGGAEDEHTLRENIAAYTRIILRPRVLVDVSKIDMST 65

Query: 104 TVLGHRISAPILVAPTSAHMLAFHEGELATARAAAAAQA-----------IKEVAASWDA 163
           T+LG+ + +PI+VAPT  H LA  EGE ATARAAA+  A           I++VA+S +A
Sbjct: 66  TLLGYTMRSPIIVAPTGGHKLAHPEGEKATARAAASCNAIMVLSFSSSCKIEDVASSCNA 125

Query: 164 VRFFQLYVFKRRDITALIVQRAETCGYKAIVVTLDTPWLGRREADMKNNYLCFTSHALMI 223
           +RF+QLYV+K R+++A +V+RAE+CG+KA+++T+DTP LGRREAD++N           +
Sbjct: 126 IRFYQLYVYKNRNVSATLVRRAESCGFKALLLTVDTPMLGRREADIRNK----------M 185

Query: 224 AYPKK-NLEGLMSVEIN----------------------SGIKWLRSITSLPILLRGILT 283
            +P+  NLEGLM+++ +                        I+WL+SITS+PI L+GI+T
Sbjct: 186 VFPRSGNLEGLMTIDDHDTTNGSQLERFARATLDPSLSWKDIEWLKSITSMPIFLKGIVT 245

Query: 284 REDATKAVEAGVDGIIVSNHGGRQLDFTPPTILVLEEAVK----EVPVVLDGGVRRGTDV 343
            EDA +AVEAGV G+IVSNHG RQLD+ P TI  LEE V+     VPV++DGG+RRGTDV
Sbjct: 246 AEDARRAVEAGVAGVIVSNHGARQLDYAPATIAALEEVVRAVAGAVPVLVDGGIRRGTDV 305

Query: 344 FKGLALGAQAILTGRAVLFGPSAKGE-GARTVLEMLKNELETTMALSGCPSIRDITRNHV 376
           FK LALGA+A++    V FG +A+GE GAR V+EML  ELE  MAL GC S+ +ITR+HV
Sbjct: 306 FKALALGARAVMXXXPVFFGLAARGEAGARHVIEMLNGELEVAMALCGCRSVGEITRSHV 365

[Close]

BLAST of CmoCh01G000140 vs. ExPASy Swiss-Prot
Match: B8B7C5 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO5 OS=Oryza sativa subsp. indica OX=39946 GN=GLO5 PE=3 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 3.5e-91
Identity = 189/366 (51.64%), Postives = 244/366 (66.67%), Query Frame = 0


Query: 46  NVNDFKELARSALPKMYYDFYAGGAEDEHTLRENIRAFHSITIRPRVLLDVSQIDMSTTV 105
           NV +++ +A+  LPKM YD+YA GAEDE TL+EN  AF  I  RPR+L+DVS+IDM+TTV
Sbjct: 6   NVTEYQAIAKQKLPKMIYDYYASGAEDEWTLQENREAFARILFRPRILIDVSKIDMATTV 65

Query: 106 LGHRISAPILVAPTSAHMLAFHEGELATARAAAAA-----------QAIKEVAASWDAVR 165
           LG +IS PI++AP++   +A  +GE ATARAA+AA            +++EVA++   +R
Sbjct: 66  LGFKISMPIMIAPSAMQKMAHPDGEYATARAASAAGTIMTLSSWATSSVEEVASTGPGIR 125

Query: 166 FFQLYVFKRRDITALIVQRAETCGYKAIVVTLDTPWLGRREADMKNNYLCFTSHALMIAY 225
           FFQLYV+K R +   +V+RAE  G+KAI +T+DTP LGRREAD+KN ++      L    
Sbjct: 126 FFQLYVYKDRRVVEQLVRRAERAGFKAIALTVDTPRLGRREADIKNRFV------LPPFL 185

Query: 226 PKKNLEGL----MSVEINSG-----------------IKWLRSITSLPILLRGILTREDA 285
             KN EGL    M    +SG                 +KWL++IT+LPIL++G++T ED 
Sbjct: 186 TLKNFEGLELGKMDQASDSGLASYVAGQIDRTLSWKDVKWLQTITTLPILVKGVITAEDT 245

Query: 286 TKAVEAGVDGIIVSNHGGRQLDFTPPTILVLEEAVK----EVPVVLDGGVRRGTDVFKGL 345
             AVE G  GIIVSNHG RQLD+ P TI  LEE VK    ++PV LDGGVRRGTDVFK L
Sbjct: 246 RLAVENGAAGIIVSNHGARQLDYVPATISALEEVVKAARGQLPVFLDGGVRRGTDVFKAL 305

Query: 346 ALGAQAILTGRAVLFGPSAKGE-GARTVLEMLKNELETTMALSGCPSIRDITRNHVRTQY 375
           ALGA  +  GR V+F  +A GE G R VL+ML++E E TMALSGC S+ DITRNHV T+ 
Sbjct: 306 ALGAAGVFIGRPVVFSLAAAGEAGVRNVLQMLRDEFELTMALSGCTSLADITRNHVITEA 365

[Close]

BLAST of CmoCh01G000140 vs. ExPASy TrEMBL
Match: A0A6J1K7A3 ((S)-2-hydroxy-acid oxidase OS=Cucurbita maxima OX=3661 GN=LOC111492921 PE=3 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 4.8e-160
Identity = 304/352 (86.36%), Postives = 320/352 (90.91%), Query Frame = 0


Query: 40  MSSKPVNVNDFKELARSALPKMYYDFYAGGAEDEHTLRENIRAFHSITIRPRVLLDVSQI 99
           MSS+PVNVNDFKELARSALPKMYYDFYAGGAEDEHTLRENIRAFHSITIRPRVL+DVSQI
Sbjct: 1   MSSEPVNVNDFKELARSALPKMYYDFYAGGAEDEHTLRENIRAFHSITIRPRVLVDVSQI 60

Query: 100 DMSTTVLGHRISAPILVAPTSAHMLAFHEGELATARAAAAAQ-----------AIKEVAA 159
           DMSTT+LGHRISAPILVAPTSAHMLAFHEGELATARAAAAAQ           +I+EVA+
Sbjct: 61  DMSTTILGHRISAPILVAPTSAHMLAFHEGELATARAAAAAQTIMVLSYSSTRSIEEVAS 120

Query: 160 SWDAVRFFQLYVFKRRDITALIVQRAETCGYKAIVVTLDTPWLGRREADMKNNYLCFTSH 219
           S DAVRFFQLYVFKRRDITALIVQRAE CGYKAIVVT+DTPWLGRREADMKN        
Sbjct: 121 SCDAVRFFQLYVFKRRDITALIVQRAERCGYKAIVVTVDTPWLGRREADMKNK------- 180

Query: 220 ALMIAYPKKNLEGLMSVEINSGIKWLRSITSLPILLRGILTREDATKAVEAGVDGIIVSN 279
             M+AYPKKNLEGLMSVEINS I+WLRSITSLPILL+GILTREDATKAVEAGVDGI+VSN
Sbjct: 181 --MVAYPKKNLEGLMSVEINSDIEWLRSITSLPILLKGILTREDATKAVEAGVDGILVSN 240

Query: 280 HGGRQLDFTPPTILVLEEAVK----EVPVVLDGGVRRGTDVFKGLALGAQAILTGRAVLF 339
           HGGRQLDFTPPTILVLEE V+    +VPV+LDGGVRRGTD+FK LALGAQA+LTGRAVLF
Sbjct: 241 HGGRQLDFTPPTILVLEEVVQAVKGKVPVLLDGGVRRGTDIFKALALGAQAVLTGRAVLF 300

Query: 340 GPSAKG-EGARTVLEMLKNELETTMALSGCPSIRDITRNHVRTQYDKLQAML 376
           G SAKG EGARTVLEMLKNELETTMALSGCPSIRDITRNHVRTQYDKLQAML
Sbjct: 301 GLSAKGEEGARTVLEMLKNELETTMALSGCPSIRDITRNHVRTQYDKLQAML 343

[Close]

BLAST of CmoCh01G000140 vs. ExPASy TrEMBL
Match: A0A6J1K9L4 ((S)-2-hydroxy-acid oxidase OS=Cucurbita maxima OX=3661 GN=LOC111492921 PE=3 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 2.5e-156
Identity = 304/373 (81.50%), Postives = 320/373 (85.79%), Query Frame = 0


Query: 40  MSSKPVNVNDFKELARSALPKMYYDFYAGGAEDEHTLRENIRAFHSITIRPRVLLDVSQI 99
           MSS+PVNVNDFKELARSALPKMYYDFYAGGAEDEHTLRENIRAFHSITIRPRVL+DVSQI
Sbjct: 1   MSSEPVNVNDFKELARSALPKMYYDFYAGGAEDEHTLRENIRAFHSITIRPRVLVDVSQI 60

Query: 100 DMSTTVLGHRISAPILVAPTSAHMLAFHEGELATARAAAAAQ-----------AIKEVAA 159
           DMSTT+LGHRISAPILVAPTSAHMLAFHEGELATARAAAAAQ           +I+EVA+
Sbjct: 61  DMSTTILGHRISAPILVAPTSAHMLAFHEGELATARAAAAAQTIMVLSYSSTRSIEEVAS 120

Query: 160 SWDAVRFFQLYVFKRRDITALIVQRAETCGYKAIVVTLDTPWLGRREADMKNNYLCFTSH 219
           S DAVRFFQLYVFKRRDITALIVQRAE CGYKAIVVT+DTPWLGRREADMKN        
Sbjct: 121 SCDAVRFFQLYVFKRRDITALIVQRAERCGYKAIVVTVDTPWLGRREADMKNK------- 180

Query: 220 ALMIAYPKKNLEGLMSVEINS---------------------GIKWLRSITSLPILLRGI 279
             M+AYPKKNLEGLMSVEINS                      I+WLRSITSLPILL+GI
Sbjct: 181 --MVAYPKKNLEGLMSVEINSEPGVQLRTFAKATVDASLSWKDIEWLRSITSLPILLKGI 240

Query: 280 LTREDATKAVEAGVDGIIVSNHGGRQLDFTPPTILVLEEAVK----EVPVVLDGGVRRGT 339
           LTREDATKAVEAGVDGI+VSNHGGRQLDFTPPTILVLEE V+    +VPV+LDGGVRRGT
Sbjct: 241 LTREDATKAVEAGVDGILVSNHGGRQLDFTPPTILVLEEVVQAVKGKVPVLLDGGVRRGT 300

Query: 340 DVFKGLALGAQAILTGRAVLFGPSAKG-EGARTVLEMLKNELETTMALSGCPSIRDITRN 376
           D+FK LALGAQA+LTGRAVLFG SAKG EGARTVLEMLKNELETTMALSGCPSIRDITRN
Sbjct: 301 DIFKALALGAQAVLTGRAVLFGLSAKGEEGARTVLEMLKNELETTMALSGCPSIRDITRN 360

[Close]

BLAST of CmoCh01G000140 vs. ExPASy TrEMBL
Match: A0A6J1KDR0 ((S)-2-hydroxy-acid oxidase OS=Cucurbita maxima OX=3661 GN=LOC111492921 PE=3 SV=1)

HSP 1 Score: 548.9 bits (1413), Expect = 1.6e-152
Identity = 304/406 (74.88%), Postives = 320/406 (78.82%), Query Frame = 0


Query: 40  MSSKPVNVNDFKELARSALPKMYYDFYAGGAEDEHTLRENIRAFHSITIRPRVLLDVSQI 99
           MSS+PVNVNDFKELARSALPKMYYDFYAGGAEDEHTLRENIRAFHSITIRPRVL+DVSQI
Sbjct: 1   MSSEPVNVNDFKELARSALPKMYYDFYAGGAEDEHTLRENIRAFHSITIRPRVLVDVSQI 60

Query: 100 DMSTTVLGHRISAPILVAPTSAHMLAFHEGELATARAAAAAQ-----------AIKEVAA 159
           DMSTT+LGHRISAPILVAPTSAHMLAFHEGELATARAAAAAQ           +I+EVA+
Sbjct: 61  DMSTTILGHRISAPILVAPTSAHMLAFHEGELATARAAAAAQTIMVLSYSSTRSIEEVAS 120

Query: 160 SWDAVRFFQLYVFKRRDITALIVQRAETCGYKAIVVTLDTPWLGRREADMKNNYLCFTSH 219
           S DAVRFFQLYVFKRRDITALIVQRAE CGYKAIVVT+DTPWLGRREADMKN        
Sbjct: 121 SCDAVRFFQLYVFKRRDITALIVQRAERCGYKAIVVTVDTPWLGRREADMKNK------- 180

Query: 220 ALMIAYPKKNLEGLMSVEI----------------------------------------- 279
             M+AYPKKNLEGLMSVEI                                         
Sbjct: 181 --MVAYPKKNLEGLMSVEINSEPGVQLRTFAKATVDASLSWKVSTESCSDSYHTLKMSAS 240

Query: 280 -------------NSGIKWLRSITSLPILLRGILTREDATKAVEAGVDGIIVSNHGGRQL 339
                        NS I+WLRSITSLPILL+GILTREDATKAVEAGVDGI+VSNHGGRQL
Sbjct: 241 LGCLISFGAYTSYNSDIEWLRSITSLPILLKGILTREDATKAVEAGVDGILVSNHGGRQL 300

Query: 340 DFTPPTILVLEEAVK----EVPVVLDGGVRRGTDVFKGLALGAQAILTGRAVLFGPSAKG 376
           DFTPPTILVLEE V+    +VPV+LDGGVRRGTD+FK LALGAQA+LTGRAVLFG SAKG
Sbjct: 301 DFTPPTILVLEEVVQAVKGKVPVLLDGGVRRGTDIFKALALGAQAVLTGRAVLFGLSAKG 360

[Close]

BLAST of CmoCh01G000140 vs. ExPASy TrEMBL
Match: A0A6J1IRU2 ((S)-2-hydroxy-acid oxidase OS=Cucurbita maxima OX=3661 GN=LOC111479940 PE=3 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 9.4e-140
Identity = 281/399 (70.43%), Postives = 313/399 (78.45%), Query Frame = 0


Query: 15  YFNFSTFSPVKAAVSSLGLT-NLKGKMSSKPVNVNDFKELARSALPKMYYDFYAGGAEDE 74
           Y NFST SP+KAA SSLG   N+KGKMSS+PVNVNDFKELAR  LPKMYYDFYAGGAEDE
Sbjct: 14  YLNFSTHSPLKAAPSSLGQNYNIKGKMSSEPVNVNDFKELARLKLPKMYYDFYAGGAEDE 73

Query: 75  HTLRENIRAFHSITIRPRVLLDVSQIDMSTTVLGHRISAPILVAPTSAHMLAFHEGELAT 134
           HTLRENI+AF  I IRPRVL+DVSQIDMSTT+LGHRISAPILVAPT+ H LAFHEGELAT
Sbjct: 74  HTLRENIQAFCRIMIRPRVLVDVSQIDMSTTILGHRISAPILVAPTAGHKLAFHEGELAT 133

Query: 135 ARAAAAAQ-----------AIKEVAASWDAVRFFQLYVFKRRDITALIVQRAETCGYKAI 194
           ARAAAAA+           +I+EVA+S +AVRFFQLY+FKRRDI+AL+VQRAE  GYKAI
Sbjct: 134 ARAAAAAKTIMILSYSSTCSIEEVASSCNAVRFFQLYIFKRRDISALLVQRAERFGYKAI 193

Query: 195 VVTLDTPWLGRREADMKNNYLCFTSHALMIAYPKKNLEGLMSVEINS------------- 254
           V+T DTP LGRREAD+KN          MIA P KNLEGLMS+++NS             
Sbjct: 194 VLTADTPRLGRREADIKNK---------MIAPPMKNLEGLMSIDVNSDQGSKLETYANET 253

Query: 255 --------GIKWLRSITSLPILLRGILTREDATKAVEAGVDGIIVSNHGGRQLDFTPPTI 314
                    I WLRS TSLPIL++GILT EDATKAVE GVDGIIVSNHG RQLDF P TI
Sbjct: 254 LDASLSWEDIMWLRSTTSLPILIKGILTHEDATKAVEGGVDGIIVSNHGARQLDFAPATI 313

Query: 315 LVLEEAVK----EVPVVLDGGVRRGTDVFKGLALGAQAILTGRAVLFGPSAKGE-GARTV 374
            VLEE V     ++PV+LDGGVRRGTDVFK LALGAQA+L GR VLFG +AKGE G RTV
Sbjct: 314 SVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLFGLAAKGEGGVRTV 373

Query: 375 LEMLKNELETTMALSGCPSIRDITRNHVRTQYDKLQAML 376
           LEMLKNELE +MALSGCP I+DITR+HVRTQ+DKL +ML
Sbjct: 374 LEMLKNELEISMALSGCPCIKDITRSHVRTQHDKLPSML 403

[Close]

BLAST of CmoCh01G000140 vs. ExPASy TrEMBL
Match: A0A6J1GUB4 ((S)-2-hydroxy-acid oxidase OS=Cucurbita moschata OX=3662 GN=LOC111457615 PE=3 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 2.0e-137
Identity = 278/397 (70.03%), Postives = 311/397 (78.34%), Query Frame = 0


Query: 17  NFSTFSPVKAAVSSLGLT-NLKGKMSSKPVNVNDFKELARSALPKMYYDFYAGGAEDEHT 76
           NF T SP+KAA SSLG   N+K KMSS+PVNVNDFKELAR  LPKMYYDFYAGGAEDEHT
Sbjct: 16  NFLTHSPLKAAPSSLGQNYNIKDKMSSEPVNVNDFKELARLKLPKMYYDFYAGGAEDEHT 75

Query: 77  LRENIRAFHSITIRPRVLLDVSQIDMSTTVLGHRISAPILVAPTSAHMLAFHEGELATAR 136
           LRENI+AF  I I+PRVL+DVSQIDMSTT+LGHRISAPILVAPT+AH LAFHEGE ATAR
Sbjct: 76  LRENIQAFCRIMIQPRVLVDVSQIDMSTTILGHRISAPILVAPTAAHKLAFHEGEQATAR 135

Query: 137 AAAAAQ-----------AIKEVAASWDAVRFFQLYVFKRRDITALIVQRAETCGYKAIVV 196
           AAAAA+           +I+EVA+S +AVRFFQLY+FKRRDI+AL+VQRAE  GYKAIV+
Sbjct: 136 AAAAAKTIMILSYSSTCSIEEVASSCNAVRFFQLYIFKRRDISALLVQRAERFGYKAIVL 195

Query: 197 TLDTPWLGRREADMKNNYLCFTSHALMIAYPKKNLEGLMSVEINS--------------- 256
           T DTP LGRREAD+KN          MIA P KNLEGL+S+++NS               
Sbjct: 196 TADTPRLGRREADIKNK---------MIAPPVKNLEGLISIDVNSDQGSKLETYANEMLD 255

Query: 257 ------GIKWLRSITSLPILLRGILTREDATKAVEAGVDGIIVSNHGGRQLDFTPPTILV 316
                  I WLRSITSLPIL++GILT EDATKAVEAGVDGIIVSNHG RQLDF P TI V
Sbjct: 256 ASLRWEDISWLRSITSLPILIKGILTHEDATKAVEAGVDGIIVSNHGARQLDFAPATISV 315

Query: 317 LEEAVK----EVPVVLDGGVRRGTDVFKGLALGAQAILTGRAVLFGPSAKGE-GARTVLE 376
           LEE V     ++PV+LDGGVRRGTDVFK LALGAQA+L GR VLFG +AKGE G RTVLE
Sbjct: 316 LEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLFGLAAKGEGGVRTVLE 375

[Close]

BLAST of CmoCh01G000140 vs. NCBI nr
Match: XP_022998202.1 (peroxisomal (S)-2-hydroxy-acid oxidase GLO3-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 573.9 bits (1478), Expect = 9.9e-160
Identity = 304/352 (86.36%), Postives = 320/352 (90.91%), Query Frame = 0


Query: 40  MSSKPVNVNDFKELARSALPKMYYDFYAGGAEDEHTLRENIRAFHSITIRPRVLLDVSQI 99
           MSS+PVNVNDFKELARSALPKMYYDFYAGGAEDEHTLRENIRAFHSITIRPRVL+DVSQI
Sbjct: 1   MSSEPVNVNDFKELARSALPKMYYDFYAGGAEDEHTLRENIRAFHSITIRPRVLVDVSQI 60

Query: 100 DMSTTVLGHRISAPILVAPTSAHMLAFHEGELATARAAAAAQ-----------AIKEVAA 159
           DMSTT+LGHRISAPILVAPTSAHMLAFHEGELATARAAAAAQ           +I+EVA+
Sbjct: 61  DMSTTILGHRISAPILVAPTSAHMLAFHEGELATARAAAAAQTIMVLSYSSTRSIEEVAS 120

Query: 160 SWDAVRFFQLYVFKRRDITALIVQRAETCGYKAIVVTLDTPWLGRREADMKNNYLCFTSH 219
           S DAVRFFQLYVFKRRDITALIVQRAE CGYKAIVVT+DTPWLGRREADMKN        
Sbjct: 121 SCDAVRFFQLYVFKRRDITALIVQRAERCGYKAIVVTVDTPWLGRREADMKNK------- 180

Query: 220 ALMIAYPKKNLEGLMSVEINSGIKWLRSITSLPILLRGILTREDATKAVEAGVDGIIVSN 279
             M+AYPKKNLEGLMSVEINS I+WLRSITSLPILL+GILTREDATKAVEAGVDGI+VSN
Sbjct: 181 --MVAYPKKNLEGLMSVEINSDIEWLRSITSLPILLKGILTREDATKAVEAGVDGILVSN 240

Query: 280 HGGRQLDFTPPTILVLEEAVK----EVPVVLDGGVRRGTDVFKGLALGAQAILTGRAVLF 339
           HGGRQLDFTPPTILVLEE V+    +VPV+LDGGVRRGTD+FK LALGAQA+LTGRAVLF
Sbjct: 241 HGGRQLDFTPPTILVLEEVVQAVKGKVPVLLDGGVRRGTDIFKALALGAQAVLTGRAVLF 300

Query: 340 GPSAKG-EGARTVLEMLKNELETTMALSGCPSIRDITRNHVRTQYDKLQAML 376
           G SAKG EGARTVLEMLKNELETTMALSGCPSIRDITRNHVRTQYDKLQAML
Sbjct: 301 GLSAKGEEGARTVLEMLKNELETTMALSGCPSIRDITRNHVRTQYDKLQAML 343

[Close]

BLAST of CmoCh01G000140 vs. NCBI nr
Match: XP_022998201.1 (peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 561.6 bits (1446), Expect = 5.1e-156
Identity = 304/373 (81.50%), Postives = 320/373 (85.79%), Query Frame = 0


Query: 40  MSSKPVNVNDFKELARSALPKMYYDFYAGGAEDEHTLRENIRAFHSITIRPRVLLDVSQI 99
           MSS+PVNVNDFKELARSALPKMYYDFYAGGAEDEHTLRENIRAFHSITIRPRVL+DVSQI
Sbjct: 1   MSSEPVNVNDFKELARSALPKMYYDFYAGGAEDEHTLRENIRAFHSITIRPRVLVDVSQI 60

Query: 100 DMSTTVLGHRISAPILVAPTSAHMLAFHEGELATARAAAAAQ-----------AIKEVAA 159
           DMSTT+LGHRISAPILVAPTSAHMLAFHEGELATARAAAAAQ           +I+EVA+
Sbjct: 61  DMSTTILGHRISAPILVAPTSAHMLAFHEGELATARAAAAAQTIMVLSYSSTRSIEEVAS 120

Query: 160 SWDAVRFFQLYVFKRRDITALIVQRAETCGYKAIVVTLDTPWLGRREADMKNNYLCFTSH 219
           S DAVRFFQLYVFKRRDITALIVQRAE CGYKAIVVT+DTPWLGRREADMKN        
Sbjct: 121 SCDAVRFFQLYVFKRRDITALIVQRAERCGYKAIVVTVDTPWLGRREADMKNK------- 180

Query: 220 ALMIAYPKKNLEGLMSVEINS---------------------GIKWLRSITSLPILLRGI 279
             M+AYPKKNLEGLMSVEINS                      I+WLRSITSLPILL+GI
Sbjct: 181 --MVAYPKKNLEGLMSVEINSEPGVQLRTFAKATVDASLSWKDIEWLRSITSLPILLKGI 240

Query: 280 LTREDATKAVEAGVDGIIVSNHGGRQLDFTPPTILVLEEAVK----EVPVVLDGGVRRGT 339
           LTREDATKAVEAGVDGI+VSNHGGRQLDFTPPTILVLEE V+    +VPV+LDGGVRRGT
Sbjct: 241 LTREDATKAVEAGVDGILVSNHGGRQLDFTPPTILVLEEVVQAVKGKVPVLLDGGVRRGT 300

Query: 340 DVFKGLALGAQAILTGRAVLFGPSAKG-EGARTVLEMLKNELETTMALSGCPSIRDITRN 376
           D+FK LALGAQA+LTGRAVLFG SAKG EGARTVLEMLKNELETTMALSGCPSIRDITRN
Sbjct: 301 DIFKALALGAQAVLTGRAVLFGLSAKGEEGARTVLEMLKNELETTMALSGCPSIRDITRN 360

[Close]

BLAST of CmoCh01G000140 vs. NCBI nr
Match: XP_022998200.1 (peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X1 [Cucurbita maxima] >XP_022998204.1 peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 548.9 bits (1413), Expect = 3.4e-152
Identity = 304/406 (74.88%), Postives = 320/406 (78.82%), Query Frame = 0


Query: 40  MSSKPVNVNDFKELARSALPKMYYDFYAGGAEDEHTLRENIRAFHSITIRPRVLLDVSQI 99
           MSS+PVNVNDFKELARSALPKMYYDFYAGGAEDEHTLRENIRAFHSITIRPRVL+DVSQI
Sbjct: 1   MSSEPVNVNDFKELARSALPKMYYDFYAGGAEDEHTLRENIRAFHSITIRPRVLVDVSQI 60

Query: 100 DMSTTVLGHRISAPILVAPTSAHMLAFHEGELATARAAAAAQ-----------AIKEVAA 159
           DMSTT+LGHRISAPILVAPTSAHMLAFHEGELATARAAAAAQ           +I+EVA+
Sbjct: 61  DMSTTILGHRISAPILVAPTSAHMLAFHEGELATARAAAAAQTIMVLSYSSTRSIEEVAS 120

Query: 160 SWDAVRFFQLYVFKRRDITALIVQRAETCGYKAIVVTLDTPWLGRREADMKNNYLCFTSH 219
           S DAVRFFQLYVFKRRDITALIVQRAE CGYKAIVVT+DTPWLGRREADMKN        
Sbjct: 121 SCDAVRFFQLYVFKRRDITALIVQRAERCGYKAIVVTVDTPWLGRREADMKNK------- 180

Query: 220 ALMIAYPKKNLEGLMSVEI----------------------------------------- 279
             M+AYPKKNLEGLMSVEI                                         
Sbjct: 181 --MVAYPKKNLEGLMSVEINSEPGVQLRTFAKATVDASLSWKVSTESCSDSYHTLKMSAS 240

Query: 280 -------------NSGIKWLRSITSLPILLRGILTREDATKAVEAGVDGIIVSNHGGRQL 339
                        NS I+WLRSITSLPILL+GILTREDATKAVEAGVDGI+VSNHGGRQL
Sbjct: 241 LGCLISFGAYTSYNSDIEWLRSITSLPILLKGILTREDATKAVEAGVDGILVSNHGGRQL 300

Query: 340 DFTPPTILVLEEAVK----EVPVVLDGGVRRGTDVFKGLALGAQAILTGRAVLFGPSAKG 376
           DFTPPTILVLEE V+    +VPV+LDGGVRRGTD+FK LALGAQA+LTGRAVLFG SAKG
Sbjct: 301 DFTPPTILVLEEVVQAVKGKVPVLLDGGVRRGTDIFKALALGAQAVLTGRAVLFGLSAKG 360

[Close]

BLAST of CmoCh01G000140 vs. NCBI nr
Match: XP_022980627.1 (peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 506.5 bits (1303), Expect = 1.9e-139
Identity = 281/399 (70.43%), Postives = 313/399 (78.45%), Query Frame = 0


Query: 15  YFNFSTFSPVKAAVSSLGLT-NLKGKMSSKPVNVNDFKELARSALPKMYYDFYAGGAEDE 74
           Y NFST SP+KAA SSLG   N+KGKMSS+PVNVNDFKELAR  LPKMYYDFYAGGAEDE
Sbjct: 14  YLNFSTHSPLKAAPSSLGQNYNIKGKMSSEPVNVNDFKELARLKLPKMYYDFYAGGAEDE 73

Query: 75  HTLRENIRAFHSITIRPRVLLDVSQIDMSTTVLGHRISAPILVAPTSAHMLAFHEGELAT 134
           HTLRENI+AF  I IRPRVL+DVSQIDMSTT+LGHRISAPILVAPT+ H LAFHEGELAT
Sbjct: 74  HTLRENIQAFCRIMIRPRVLVDVSQIDMSTTILGHRISAPILVAPTAGHKLAFHEGELAT 133

Query: 135 ARAAAAAQ-----------AIKEVAASWDAVRFFQLYVFKRRDITALIVQRAETCGYKAI 194
           ARAAAAA+           +I+EVA+S +AVRFFQLY+FKRRDI+AL+VQRAE  GYKAI
Sbjct: 134 ARAAAAAKTIMILSYSSTCSIEEVASSCNAVRFFQLYIFKRRDISALLVQRAERFGYKAI 193

Query: 195 VVTLDTPWLGRREADMKNNYLCFTSHALMIAYPKKNLEGLMSVEINS------------- 254
           V+T DTP LGRREAD+KN          MIA P KNLEGLMS+++NS             
Sbjct: 194 VLTADTPRLGRREADIKNK---------MIAPPMKNLEGLMSIDVNSDQGSKLETYANET 253

Query: 255 --------GIKWLRSITSLPILLRGILTREDATKAVEAGVDGIIVSNHGGRQLDFTPPTI 314
                    I WLRS TSLPIL++GILT EDATKAVE GVDGIIVSNHG RQLDF P TI
Sbjct: 254 LDASLSWEDIMWLRSTTSLPILIKGILTHEDATKAVEGGVDGIIVSNHGARQLDFAPATI 313

Query: 315 LVLEEAVK----EVPVVLDGGVRRGTDVFKGLALGAQAILTGRAVLFGPSAKGE-GARTV 374
            VLEE V     ++PV+LDGGVRRGTDVFK LALGAQA+L GR VLFG +AKGE G RTV
Sbjct: 314 SVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLFGLAAKGEGGVRTV 373

Query: 375 LEMLKNELETTMALSGCPSIRDITRNHVRTQYDKLQAML 376
           LEMLKNELE +MALSGCP I+DITR+HVRTQ+DKL +ML
Sbjct: 374 LEMLKNELEISMALSGCPCIKDITRSHVRTQHDKLPSML 403

[Close]

BLAST of CmoCh01G000140 vs. NCBI nr
Match: XP_023527007.1 (peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 500.0 bits (1286), Expect = 1.8e-137
Identity = 282/413 (68.28%), Postives = 318/413 (77.00%), Query Frame = 0


Query: 1   MYHVILINTFIFGFYFNFSTFSPVKAAVSSLGLT-NLKGKMSSKPVNVNDFKELARSALP 60
           M HV +    +   Y NFST SP+KAA SSLG   N+K KMSS+PVNVNDFKELAR  LP
Sbjct: 1   MVHVFISKISVVN-YLNFSTRSPLKAAPSSLGQNYNIKDKMSSEPVNVNDFKELARLKLP 60

Query: 61  KMYYDFYAGGAEDEHTLRENIRAFHSITIRPRVLLDVSQIDMSTTVLGHRISAPILVAPT 120
           KMYYDFYAGGAEDEHTLRENI+AF  I I+PRVL+DVSQID+STT+LGHRISAPILVAPT
Sbjct: 61  KMYYDFYAGGAEDEHTLRENIQAFCRIMIQPRVLVDVSQIDLSTTILGHRISAPILVAPT 120

Query: 121 SAHMLAFHEGELATARAAAAAQ-----------AIKEVAASWDAVRFFQLYVFKRRDITA 180
           +AH LAFHEGE ATARAAAAA+           +I+EVA+S +AVRFFQLY+FKRRDI+A
Sbjct: 121 AAHKLAFHEGEQATARAAAAAKTIMILSYSSTCSIEEVASSCNAVRFFQLYIFKRRDISA 180

Query: 181 LIVQRAETCGYKAIVVTLDTPWLGRREADMKNNYLCFTSHALMIAYPKKNLEGLMSVEIN 240
           L+VQRAE  GYKAIV+T DTP LGRREAD+KN          MIA P KNLEGL+S+++N
Sbjct: 181 LLVQRAERFGYKAIVLTADTPRLGRREADIKNK---------MIAPPVKNLEGLISIDVN 240

Query: 241 S---------------------GIKWLRSITSLPILLRGILTREDATKAVEAGVDGIIVS 300
           S                      I WLRSITSLPIL++GILT EDATKAVEAGVDGIIVS
Sbjct: 241 SDQGSKLETYANEMLDASLRWEDISWLRSITSLPILIKGILTHEDATKAVEAGVDGIIVS 300

Query: 301 NHGGRQLDFTPPTILVLEEAVK----EVPVVLDGGVRRGTDVFKGLALGAQAILTGRAVL 360
           NHG RQLDF P TI VLEE V     ++PV+LDGGVRRGTDVFK LALGAQA+L GR VL
Sbjct: 301 NHGARQLDFAPATISVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVL 360

Query: 361 FGPSAKGE-GARTVLEMLKNELETTMALSGCPSIRDITRNHVRTQYDKLQAML 376
           FG +AKGE G RTVLEMLKNELE +MALSGCP I+DITR+HVRT YDKL +ML
Sbjct: 361 FGLAAKGEGGVRTVLEMLKNELEISMALSGCPCIKDITRSHVRTPYDKLPSML 403

[Close]

BLAST of CmoCh01G000140 vs. TAIR 10
Match: AT3G14150.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 409.8 bits (1052), Expect = 2.3e-114
Identity = 221/368 (60.05%), Postives = 270/368 (73.37%), Query Frame = 0


Query: 45  VNVNDFKELARSALPKMYYDFYAGGAEDEHTLRENIRAFHSITIRPRVLLDVSQIDMSTT 104
           VNV++F+ELA+ ALPKMYYDFY GGAED+HTL EN++AF  I  RPRVL+DVS+IDMST 
Sbjct: 5   VNVDEFQELAKQALPKMYYDFYNGGAEDQHTLNENVQAFRRIMFRPRVLVDVSKIDMSTK 64

Query: 105 VLGHRISAPILVAPTSAHMLAFHEGELATARAAAAAQAI-----------KEVAASWDAV 164
           +LG+ ISAPI++APT  H LA  EGE ATA+AAAA   I           +E+A+S +AV
Sbjct: 65  ILGYPISAPIMIAPTGNHKLAHPEGETATAKAAAACNTIMIVSYMSSCTFEEIASSCNAV 124

Query: 165 RFFQLYVFKRRDITALIVQRAETCGYKAIVVTLDTPWLGRREADMKNNYLCFTSHALMIA 224
           RF Q+YV+KRRDITA +V+RAE  G+KAIV+T+D P LGRREAD+KN          MI+
Sbjct: 125 RFLQIYVYKRRDITAQVVKRAEKAGFKAIVLTVDVPRLGRREADIKNK---------MIS 184

Query: 225 YPKKNLEGLMSVEIN---------------------SGIKWLRSITSLPILLRGILTRED 284
              KN EGL S E+                        I+WLRSIT LPIL++GILTRED
Sbjct: 185 PQLKNFEGLFSTEVRPSKGSGVQAFASRAFDASFSWKDIEWLRSITELPILVKGILTRED 244

Query: 285 ATKAVEAGVDGIIVSNHGGRQLDFTPPTILVLEEAVK----EVPVVLDGGVRRGTDVFKG 344
           A KAVEAGVDGIIVSNHGGRQLD++P TI VLEE V+     +PV+LDGGVRRGTDVFK 
Sbjct: 245 ALKAVEAGVDGIIVSNHGGRQLDYSPATITVLEEVVQVVRGRIPVLLDGGVRRGTDVFKA 304

Query: 345 LALGAQAILTGRAVLFGPSAKGE-GARTVLEMLKNELETTMALSGCPSIRDITRNHVRTQ 376
           LALGAQA+L GR +++G +AKGE G + V++MLKNE E TMALSGCP+I DITRNHVRT+
Sbjct: 305 LALGAQAVLIGRPIIYGLAAKGEDGVKKVIDMLKNEFEITMALSGCPTIDDITRNHVRTE 363

[Close]

BLAST of CmoCh01G000140 vs. TAIR 10
Match: AT3G14150.2 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 409.8 bits (1052), Expect = 2.3e-114
Identity = 221/368 (60.05%), Postives = 270/368 (73.37%), Query Frame = 0


Query: 45  VNVNDFKELARSALPKMYYDFYAGGAEDEHTLRENIRAFHSITIRPRVLLDVSQIDMSTT 104
           VNV++F+ELA+ ALPKMYYDFY GGAED+HTL EN++AF  I  RPRVL+DVS+IDMST 
Sbjct: 5   VNVDEFQELAKQALPKMYYDFYNGGAEDQHTLNENVQAFRRIMFRPRVLVDVSKIDMSTK 64

Query: 105 VLGHRISAPILVAPTSAHMLAFHEGELATARAAAAAQAI-----------KEVAASWDAV 164
           +LG+ ISAPI++APT  H LA  EGE ATA+AAAA   I           +E+A+S +AV
Sbjct: 65  ILGYPISAPIMIAPTGNHKLAHPEGETATAKAAAACNTIMIVSYMSSCTFEEIASSCNAV 124

Query: 165 RFFQLYVFKRRDITALIVQRAETCGYKAIVVTLDTPWLGRREADMKNNYLCFTSHALMIA 224
           RF Q+YV+KRRDITA +V+RAE  G+KAIV+T+D P LGRREAD+KN          MI+
Sbjct: 125 RFLQIYVYKRRDITAQVVKRAEKAGFKAIVLTVDVPRLGRREADIKNK---------MIS 184

Query: 225 YPKKNLEGLMSVEIN---------------------SGIKWLRSITSLPILLRGILTRED 284
              KN EGL S E+                        I+WLRSIT LPIL++GILTRED
Sbjct: 185 PQLKNFEGLFSTEVRPSKGSGVQAFASRAFDASFSWKDIEWLRSITELPILVKGILTRED 244

Query: 285 ATKAVEAGVDGIIVSNHGGRQLDFTPPTILVLEEAVK----EVPVVLDGGVRRGTDVFKG 344
           A KAVEAGVDGIIVSNHGGRQLD++P TI VLEE V+     +PV+LDGGVRRGTDVFK 
Sbjct: 245 ALKAVEAGVDGIIVSNHGGRQLDYSPATITVLEEVVQVVRGRIPVLLDGGVRRGTDVFKA 304

Query: 345 LALGAQAILTGRAVLFGPSAKGE-GARTVLEMLKNELETTMALSGCPSIRDITRNHVRTQ 376
           LALGAQA+L GR +++G +AKGE G + V++MLKNE E TMALSGCP+I DITRNHVRT+
Sbjct: 305 LALGAQAVLIGRPIIYGLAAKGEDGVKKVIDMLKNEFEITMALSGCPTIDDITRNHVRTE 363

[Close]

BLAST of CmoCh01G000140 vs. TAIR 10
Match: AT3G14130.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 406.0 bits (1042), Expect = 3.3e-113
Identity = 218/368 (59.24%), Postives = 274/368 (74.46%), Query Frame = 0


Query: 45  VNVNDFKELARSALPKMYYDFYAGGAEDEHTLRENIRAFHSITIRPRVLLDVSQIDMSTT 104
           VNV++F+ELA+ ALPKMYYDFY GGAED+HTL EN++AF  I  RPRVL+DVS IDMST+
Sbjct: 5   VNVDEFQELAKQALPKMYYDFYNGGAEDQHTLNENVQAFRRIMFRPRVLVDVSNIDMSTS 64

Query: 105 VLGHRISAPILVAPTSAHMLAFHEGELATARAAAAAQ-----------AIKEVAASWDAV 164
           +LG+ ISAPI++APT+ H LA  +GE+ATA+AAAA              I+EVA+S +AV
Sbjct: 65  MLGYPISAPIMIAPTAMHKLAHPKGEIATAKAAAACNTIMIVSFMSTCTIEEVASSCNAV 124

Query: 165 RFFQLYVFKRRDITALIVQRAETCGYKAIVVTLDTPWLGRREADMKNNYLCFTSHALMIA 224
           RF Q+YV+KRRD+TA IV+RAE  G+KAIV+T+D P LGRREAD+KN          MI+
Sbjct: 125 RFLQIYVYKRRDVTAQIVKRAEKAGFKAIVLTVDVPRLGRREADIKNK---------MIS 184

Query: 225 YPKKNLEGLMSVEI--NSG-------------------IKWLRSITSLPILLRGILTRED 284
              KN EGL+S E+  N G                   I+WLRSIT LPIL++G+LTRED
Sbjct: 185 PQLKNFEGLVSTEVRPNEGSGVEAFASSAFDASLSWKDIEWLRSITKLPILVKGLLTRED 244

Query: 285 ATKAVEAGVDGIIVSNHGGRQLDFTPPTILVLEEAVK----EVPVVLDGGVRRGTDVFKG 344
           A KAVEAGVDGI+VSNHG RQLD++P TI VLEE V      +PV+LDGGVRRGTDVFK 
Sbjct: 245 ALKAVEAGVDGIVVSNHGARQLDYSPATITVLEEVVHAVKGRIPVLLDGGVRRGTDVFKA 304

Query: 345 LALGAQAILTGRAVLFGPSAKGE-GARTVLEMLKNELETTMALSGCPSIRDITRNHVRTQ 376
           LALGAQA+L GR +++G +AKGE G + V++MLKNE E TMALSGCP+I D+TRNHVRT+
Sbjct: 305 LALGAQAVLIGRPIVYGLAAKGEDGVKKVIDMLKNEFEITMALSGCPTIDDVTRNHVRTE 363

[Close]

BLAST of CmoCh01G000140 vs. TAIR 10
Match: AT4G18360.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 334.0 bits (855), Expect = 1.6e-91
Identity = 184/361 (50.97%), Postives = 242/361 (67.04%), Query Frame = 0


Query: 46  NVNDFKELARSALPKMYYDFYAGGAEDEHTLRENIRAFHSITIRPRVLLDVSQIDMSTTV 105
           NV +++++A+  LPKM YD+YA GAED+ TL+EN  AF  I  RPR+L+DVS+ID+STTV
Sbjct: 5   NVMEYEKIAKEKLPKMVYDYYASGAEDQWTLQENRNAFSRILFRPRILIDVSKIDVSTTV 64

Query: 106 LGHRISAPILVAPTSAHMLAFHEGELATARAAAAAQ-----------AIKEVAASWDAVR 165
           LG  IS PI++APT+   +A  +GELATARA +AA            +++EVA++   +R
Sbjct: 65  LGFNISMPIMIAPTAMQKMAHPDGELATARATSAAGTIMTLSSWATCSVEEVASTGPGIR 124

Query: 166 FFQLYVFKRRDITALIVQRAETCGYKAIVVTLDTPWLGRREADMKNNYLCFTSHALMIAY 225
           FFQLYV+K R++   +V+RAE  G+KAI +T+DTP LGRRE+D+KN +      AL    
Sbjct: 125 FFQLYVYKDRNVVIQLVKRAEEAGFKAIALTVDTPRLGRRESDIKNRF------ALPRGL 184

Query: 226 PKKNLEGLMSVEIN---------------------SGIKWLRSITSLPILLRGILTREDA 285
             KN EGL   +I+                       IKWL+SITSLPIL++G++T EDA
Sbjct: 185 TLKNFEGLDLGKIDKTNDSGLASYVAGQVDQSLSWKDIKWLQSITSLPILVKGVITAEDA 244

Query: 286 TKAVEAGVDGIIVSNHGGRQLDFTPPTILVLEEAVK----EVPVVLDGGVRRGTDVFKGL 345
             AVE G  GIIVSNHG RQLD+ P TI+ LEE VK     +PV LDGGVRRGTDVFK L
Sbjct: 245 RIAVEYGAAGIIVSNHGARQLDYVPATIVALEEVVKAVEGRIPVFLDGGVRRGTDVFKAL 304

Query: 346 ALGAQAILTGRAVLFGPSAKGE-GARTVLEMLKNELETTMALSGCPSIRDITRNHVRTQY 370
           ALGA  +  GR  LF  +A GE G R +L+ML++E E TMALSGC S+R+I+R H++T +
Sbjct: 305 ALGASGVFVGRPSLFSLAADGEAGVRKMLQMLRDEFELTMALSGCRSLREISRTHIKTDW 359

[Close]

BLAST of CmoCh01G000140 vs. TAIR 10
Match: AT3G14420.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 333.6 bits (854), Expect = 2.1e-91
Identity = 184/361 (50.97%), Postives = 244/361 (67.59%), Query Frame = 0


Query: 46  NVNDFKELARSALPKMYYDFYAGGAEDEHTLRENIRAFHSITIRPRVLLDVSQIDMSTTV 105
           NV ++  +A+  LPKM YD+YA GAED+ TL+EN  AF  I  RPR+L+DVS+IDM+TTV
Sbjct: 5   NVTEYDAIAKQKLPKMVYDYYASGAEDQWTLQENRNAFARILFRPRILIDVSKIDMTTTV 64

Query: 106 LGHRISAPILVAPTSAHMLAFHEGELATARAAAAA-----------QAIKEVAASWDAVR 165
           LG +IS PI+VAPT+   +A  +GE ATARAA+AA            +++EVA++   +R
Sbjct: 65  LGFKISMPIMVAPTAMQKMAHPDGEYATARAASAAGTIMTLSSWATSSVEEVASTGPGIR 124

Query: 166 FFQLYVFKRRDITALIVQRAETCGYKAIVVTLDTPWLGRREADMKNNYLCFTSHALMIAY 225
           FFQLYV+K R++   +V+RAE  G+KAI +T+DTP LGRRE+D+KN +    +  L    
Sbjct: 125 FFQLYVYKNRNVVEQLVRRAERAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTL---- 184

Query: 226 PKKNLEGL----MSVEINSG-----------------IKWLRSITSLPILLRGILTREDA 285
             KN EGL    M    +SG                 ++WL++IT LPIL++G+LT EDA
Sbjct: 185 --KNFEGLDLGKMDEANDSGLASYVAGQIDRTLSWKDVQWLQTITKLPILVKGVLTGEDA 244

Query: 286 TKAVEAGVDGIIVSNHGGRQLDFTPPTILVLEEAVK----EVPVVLDGGVRRGTDVFKGL 345
             A++AG  GIIVSNHG RQLD+ P TI  LEE VK     +PV LDGGVRRGTDVFK L
Sbjct: 245 RIAIQAGAAGIIVSNHGARQLDYVPATISALEEVVKATQGRIPVFLDGGVRRGTDVFKAL 304

Query: 346 ALGAQAILTGRAVLFGPSAKGE-GARTVLEMLKNELETTMALSGCPSIRDITRNHVRTQY 370
           ALGA  I  GR V+F  +A+GE G R VL+ML++E E TMALSGC S+++I+RNH+ T++
Sbjct: 305 ALGASGIFIGRPVVFSLAAEGEAGVRKVLQMLRDEFELTMALSGCRSLKEISRNHITTEW 359

The following BLAST results are available for this feature:

BLAST of CmoCh01G000140 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q24JJ8	3.2e-113	60.05	Peroxisomal (S)-2-hydroxy-acid oxidase GLO3 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
Q9LJH5	4.7e-112	59.24	Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
Q8H3I4	1.3e-101	53.37	Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Oryza sativa subsp. japonica OX=3...	[more]
B8B8K5	2.4e-100	52.83	Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Oryza sativa subsp. indica OX=399...	[more]
B8B7C5	3.5e-91	51.64	Peroxisomal (S)-2-hydroxy-acid oxidase GLO5 OS=Oryza sativa subsp. indica OX=399...	[more]

BLAST of CmoCh01G000140 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K7A3	4.8e-160	86.36	(S)-2-hydroxy-acid oxidase OS=Cucurbita maxima OX=3661 GN=LOC111492921 PE=3 SV=1	[more]
A0A6J1K9L4	2.5e-156	81.50	(S)-2-hydroxy-acid oxidase OS=Cucurbita maxima OX=3661 GN=LOC111492921 PE=3 SV=1	[more]
A0A6J1KDR0	1.6e-152	74.88	(S)-2-hydroxy-acid oxidase OS=Cucurbita maxima OX=3661 GN=LOC111492921 PE=3 SV=1	[more]
A0A6J1IRU2	9.4e-140	70.43	(S)-2-hydroxy-acid oxidase OS=Cucurbita maxima OX=3661 GN=LOC111479940 PE=3 SV=1	[more]
A0A6J1GUB4	2.0e-137	70.03	(S)-2-hydroxy-acid oxidase OS=Cucurbita moschata OX=3662 GN=LOC111457615 PE=3 SV...	[more]

BLAST of CmoCh01G000140 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022998202.1	9.9e-160	86.36	peroxisomal (S)-2-hydroxy-acid oxidase GLO3-like isoform X3 [Cucurbita maxima]	[more]
XP_022998201.1	5.1e-156	81.50	peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X2 [Cucurbita maxima]	[more]
XP_022998200.1	3.4e-152	74.88	peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X1 [Cucurbita maxima] >...	[more]
XP_022980627.1	1.9e-139	70.43	peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X2 [Cucurbita maxima]	[more]
XP_023527007.1	1.8e-137	68.28	peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X1 [Cucurbita pepo subs...	[more]

BLAST of CmoCh01G000140 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G14150.1	2.3e-114	60.05	Aldolase-type TIM barrel family protein	[more]
AT3G14150.2	2.3e-114	60.05	Aldolase-type TIM barrel family protein	[more]
AT3G14130.1	3.3e-113	59.24	Aldolase-type TIM barrel family protein	[more]
AT4G18360.1	1.6e-91	50.97	Aldolase-type TIM barrel family protein	[more]
AT3G14420.1	2.1e-91	50.97	Aldolase-type TIM barrel family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 43..375 e-value: 1.4E-120 score: 404.4
IPR012133	Alpha-hydroxy acid dehydrogenase, FMN-dependent	PIRSF	PIRSF000138	Al-hdrx_acd_dh	coord: 30..369 e-value: 8.4E-107 score: 355.3
IPR012133	Alpha-hydroxy acid dehydrogenase, FMN-dependent	CDD	cd02809	alpha_hydroxyacid_oxid_FMN	coord: 48..361 e-value: 4.44842E-126 score: 362.92
IPR000262	FMN-dependent dehydrogenase	PFAM	PF01070	FMN_dh	coord: 54..365 e-value: 3.6E-101 score: 338.7
None	No IPR available	PIRSR	PIRSR000138-2	PIRSR000138-2	coord: 39..366 e-value: 1.5E-105 score: 351.0
None	No IPR available	PANTHER	PTHR10578	S -2-HYDROXY-ACID OXIDASE-RELATED	coord: 231..366
None	No IPR available	PANTHER	PTHR10578:SF116	PEROXISOMAL (S)-2-HYDROXY-ACID OXIDASE GLO4-LIKE	coord: 231..366
None	No IPR available	PANTHER	PTHR10578:SF116	PEROXISOMAL (S)-2-HYDROXY-ACID OXIDASE GLO4-LIKE	coord: 40..227
None	No IPR available	PANTHER	PTHR10578	S -2-HYDROXY-ACID OXIDASE-RELATED	coord: 40..227
None	No IPR available	SUPERFAMILY	51395	FMN-linked oxidoreductases	coord: 43..366
IPR008259	FMN-dependent alpha-hydroxy acid dehydrogenase, active site	PROSITE	PS00557	FMN_HYDROXY_ACID_DH_1	coord: 267..273
IPR037396	FMN hydroxy acid dehydrogenase domain	PROSITE	PS51349	FMN_HYDROXY_ACID_DH_2	coord: 39..369 score: 83.798752

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh01G000140.1	CmoCh01G000140.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009854	oxidative photosynthetic carbon pathway
cellular_component	GO:0005777	peroxisome
molecular_function	GO:0010181	FMN binding
molecular_function	GO:0003973	(S)-2-hydroxy-acid oxidase activity
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0016491	oxidoreductase activity