Cmc12g0331211 (gene) Melon (Charmono) v1.1

Overview
NameCmc12g0331211
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionSphingolipid delta(4)-desaturase DES1-like
LocationCMiso1.1chr12: 21973827 .. 21976638 (-)
RNA-Seq ExpressionCmc12g0331211
SyntenyCmc12g0331211
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGGTCGAATAGAAAAGAATAGAATCGAATATAGATAGATACCATTCCCACCAGCGGCAAGTTGGCAATGGCAACGCTACGAAGACGGCGACGTATAAAACAGCGTCCGGTCGAACGCAACGTCCTTTTCTAAATTTAGGAGTCACCTTCGTTTCCATTTTCCTTTCCATATTTGGTCGTCTTCAATCCTCCGCCCATTCCTAAATCTTCAACAACCTTCCTCTATAACTATCCCTTCCTTTCTCTTCCTCTCAATCACCATCCAATTTCATCTTCACCAATCAGCACCACACCCCAGGAGACGCAAATTCTTCCGGGCATCTTCTGGGAGCAACGACCACCGGGAATCACTCACATACACAATGCAAGGTGGTGCAGAGAGAGATGATGGCGTTATGGCCACTGACTTCTTCTGGTCTTACACCGATGAACCTCACGCTTCCAGGAGACGACAAATTCTCTCTCAGTACCCTCAGATCCGGGAGCTCTTCGGTCCTGACCCCTGGGCTTTCTTCAAGGTTTTTCATTTCTTCTCTCTCTTGATTCTGAGAATGTAGAGAGTTGTGGTTATTTTCTTGTCAGGATGTTTTGATTTCGTGTTTAGATCTGTATTGCTGGTCTCTACACATGTCAATGCCTGAATTTGATTTGTGTATATTTGTTTTTATGTTTGATTTTGAACCTCATCTCTGCTACATTGCTGCGTTTTAATTTGTTTTTGTTTTTCTTTTATCATTCTGATTACTCGTATTTGTTGCATCCATCGAAAATCTACATTTGAGATTCGGAACACTCGTAATTGATGGATCTGTCCACTTCGCTGTTCTTTTTGCGCTGGTTCAGTTATTTTTTTTTTTTGCTACATTCTTGTATCGTTTATTTATTTGTTTGTTTTTTTGTTCCTTTCTTCCTTCCTTGTAGTACGAACGCTTGTCTAAACTTTCCTGCCTTTTCAACTATTAGGCAAGCTTTGATTCAGGATTGTTTTGATTTAAACTTTATTTAAAGTTACCCTTCTTTTCTTATTTGGAATTTAAAAGAATACCCTGGAGGGAAATTAACCCTCAACCTTTTTCACTACGAAATGGTAAAGTTTAGTAGTACTCGATGAATTTTGTTGTTTGCTCTCTCGAGTTTTGGTAAATGTTTAGGGAGGAAATTAGCCCTCCACCCTTATTTTCTTGGTTGCCTGGCTCTAATATCTGATTTTAGCAGATGTTTAATCCTTTGATTAGGGTAGTCCATTGCAGCAGTTTAGGGCTTGTATAGTTTCAAATGAGACCATTTATGAAATCAGAAGAATAATTGTTGAAGTTAAGTATTTAGACTAATGCTTCCTGCTGTTGTAAAGGGAAGGCTGATTTTTTTTTTATTGCTTGTTGTATTTGTTAAGAAGAGCAATTGATTGTAATGATTTAACCAACTGCGTTGGCATTTTGATTCATTTGATGAAAAAAGTAAAGTCATGATCACGAAGAATGAGAACTTGGTGTGAACAGCCATTTATACATTCACGAGCATGACCTTGTAGTCATAAAGTTCAAATTAACTATTGTAGTTTGAAGAACCAATCATTAACTTATTCTTACAGTATAAAAAGTTGGTCACTTTTTACTTCTCGTTCTTATTCTCGCAATCTTGCAGATTTCTATGGTGGTTCTGCTTCAGTTATGGACTGCTACGACTCTTCACAGTGCAGACTGGTTAAAGATATTGGCAATAGCCTACTTCTTTGGCTCTTTTCTCAACCACAACCTATTTTTAGCCATTCACGAGCTCAGTCACAACCTTGCCTTCTCAACACCTGACTACAACAAGTGGCTCGGGATTTTTGCTAATCTCCCCATTGGAGTGCCCATGTCCATCACATTTCAAAAGTATCACCTCGAGCACCATCGCTTCCAAGGAGTGGATGGCATAGATATGGACGTTCCGAGCCAGACCGAAGCTCATCTGGTGACTAATGTTGTCTCGAAAGCTATATGGGTTATCTTCCAACTCTTCTTCTATGCACTCCGCCCATTGTTTCTCAAACCAAAGCCCCCAGGTCACTGGGAGTTCATCAACTTCATTATTCAGATAGCTCTTGATGTTGCCATGGTTTACTTCTGGAGTTGGAAGTCTTTTGCTTACCTGATCCTCTCCACATTCGTCGGTGGTGGGATGCATCCAATGGCCGGTCACTTCATTTCCGAACATTATGTCTTCAATCCCAAACAGGAGACATATTCTTACTACGGTCCACTTAATCTTCTTACATGGAGTGTAGGTTACCACAATGAACACCATGACTTCCCCAGAATTCCAGGAAGCAAGCTTTACAAAGTGAAGGAGATTGCACCTGAATACTACAATGAATTAGATTCATACAAATCGTGGAGCCAGGTGATATACATGTACATTATGGACAAGACAGTGGGGCCATTCAGTAGGATGAAGAGAATGCCAAGGAAGTCTGAGTAGTTGATCCTTTCTCCTCTCCCCTTTTAGATATTTTTTGTTCGATTCTTTCTCCATCTTTTCTGGTGGATCATTCTCTTTGAGCTGGTTGTGCCCTTGTTTTATTTACTTACATGTGGAGAGTTTTCTTTATTCTATTTAGATTGTATAGTAGCGAGGCATGTTCACTGTACTGTATTCGATTCTTCATGGCTTTTTGAGGGGTTGGTTTGTAAAAGGTGCCAAGAATTAGCGAACCTAGATTCATATGTTTGATATTTTGGTTAATTTATAAATTTAGTGCTGACTGTACCTACGTATTTTTATCTACATTGTTTCGGAAATCCTAGATGAGAAAAGCCGTGATGCTAGAT

mRNA sequence

GGGTCGAATAGAAAAGAATAGAATCGAATATAGATAGATACCATTCCCACCAGCGGCAAGTTGGCAATGGCAACGCTACGAAGACGGCGACGTATAAAACAGCGTCCGGTCGAACGCAACGTCCTTTTCTAAATTTAGGAGTCACCTTCGTTTCCATTTTCCTTTCCATATTTGGTCGTCTTCAATCCTCCGCCCATTCCTAAATCTTCAACAACCTTCCTCTATAACTATCCCTTCCTTTCTCTTCCTCTCAATCACCATCCAATTTCATCTTCACCAATCAGCACCACACCCCAGGAGACGCAAATTCTTCCGGGCATCTTCTGGGAGCAACGACCACCGGGAATCACTCACATACACAATGCAAGGTGGTGCAGAGAGAGATGATGGCGTTATGGCCACTGACTTCTTCTGGTCTTACACCGATGAACCTCACGCTTCCAGGAGACGACAAATTCTCTCTCAGTACCCTCAGATCCGGGAGCTCTTCGGTCCTGACCCCTGGGCTTTCTTCAAGATTTCTATGGTGGTTCTGCTTCAGTTATGGACTGCTACGACTCTTCACAGTGCAGACTGGTTAAAGATATTGGCAATAGCCTACTTCTTTGGCTCTTTTCTCAACCACAACCTATTTTTAGCCATTCACGAGCTCAGTCACAACCTTGCCTTCTCAACACCTGACTACAACAAGTGGCTCGGGATTTTTGCTAATCTCCCCATTGGAGTGCCCATGTCCATCACATTTCAAAAGTATCACCTCGAGCACCATCGCTTCCAAGGAGTGGATGGCATAGATATGGACGTTCCGAGCCAGACCGAAGCTCATCTGGTGACTAATGTTGTCTCGAAAGCTATATGGGTTATCTTCCAACTCTTCTTCTATGCACTCCGCCCATTGTTTCTCAAACCAAAGCCCCCAGGTCACTGGGAGTTCATCAACTTCATTATTCAGATAGCTCTTGATGTTGCCATGGTTTACTTCTGGAGTTGGAAGTCTTTTGCTTACCTGATCCTCTCCACATTCGTCGGTGGTGGGATGCATCCAATGGCCGGTCACTTCATTTCCGAACATTATGTCTTCAATCCCAAACAGGAGACATATTCTTACTACGGTCCACTTAATCTTCTTACATGGAGTGTAGGTTACCACAATGAACACCATGACTTCCCCAGAATTCCAGGAAGCAAGCTTTACAAAGTGAAGGAGATTGCACCTGAATACTACAATGAATTAGATTCATACAAATCGTGGAGCCAGGTGATATACATGTACATTATGGACAAGACAGTGGGGCCATTCAGTAGGATGAAGAGAATGCCAAGGAAGTCTGAGTAGTTGATCCTTTCTCCTCTCCCCTTTTAGATATTTTTTGTTCGATTCTTTCTCCATCTTTTCTGGTGGATCATTCTCTTTGAGCTGGTTGTGCCCTTGTTTTATTTACTTACATGTGGAGAGTTTTCTTTATTCTATTTAGATTGTATAGTAGCGAGGCATGTTCACTGTACTGTATTCGATTCTTCATGGCTTTTTGAGGGGTTGGTTTGTAAAAGGTGCCAAGAATTAGCGAACCTAGATTCATATGTTTGATATTTTGGTTAATTTATAAATTTAGTGCTGACTGTACCTACGTATTTTTATCTACATTGTTTCGGAAATCCTAGATGAGAAAAGCCGTGATGCTAGAT

Coding sequence (CDS)

ATGCAAGGTGGTGCAGAGAGAGATGATGGCGTTATGGCCACTGACTTCTTCTGGTCTTACACCGATGAACCTCACGCTTCCAGGAGACGACAAATTCTCTCTCAGTACCCTCAGATCCGGGAGCTCTTCGGTCCTGACCCCTGGGCTTTCTTCAAGATTTCTATGGTGGTTCTGCTTCAGTTATGGACTGCTACGACTCTTCACAGTGCAGACTGGTTAAAGATATTGGCAATAGCCTACTTCTTTGGCTCTTTTCTCAACCACAACCTATTTTTAGCCATTCACGAGCTCAGTCACAACCTTGCCTTCTCAACACCTGACTACAACAAGTGGCTCGGGATTTTTGCTAATCTCCCCATTGGAGTGCCCATGTCCATCACATTTCAAAAGTATCACCTCGAGCACCATCGCTTCCAAGGAGTGGATGGCATAGATATGGACGTTCCGAGCCAGACCGAAGCTCATCTGGTGACTAATGTTGTCTCGAAAGCTATATGGGTTATCTTCCAACTCTTCTTCTATGCACTCCGCCCATTGTTTCTCAAACCAAAGCCCCCAGGTCACTGGGAGTTCATCAACTTCATTATTCAGATAGCTCTTGATGTTGCCATGGTTTACTTCTGGAGTTGGAAGTCTTTTGCTTACCTGATCCTCTCCACATTCGTCGGTGGTGGGATGCATCCAATGGCCGGTCACTTCATTTCCGAACATTATGTCTTCAATCCCAAACAGGAGACATATTCTTACTACGGTCCACTTAATCTTCTTACATGGAGTGTAGGTTACCACAATGAACACCATGACTTCCCCAGAATTCCAGGAAGCAAGCTTTACAAAGTGAAGGAGATTGCACCTGAATACTACAATGAATTAGATTCATACAAATCGTGGAGCCAGGTGATATACATGTACATTATGGACAAGACAGTGGGGCCATTCAGTAGGATGAAGAGAATGCCAAGGAAGTCTGAGTAG

Protein sequence

MQGGAERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISMVVLLQLWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDGIDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIYMYIMDKTVGPFSRMKRMPRKSE
Homology
BLAST of Cmc12g0331211 vs. NCBI nr
Match: XP_008442400.1 (PREDICTED: sphingolipid delta(4)-desaturase DES1-like [Cucumis melo] >KAA0056978.1 sphingolipid delta(4)-desaturase DES1-like [Cucumis melo var. makuwa] >TYK26405.1 sphingolipid delta(4)-desaturase DES1-like [Cucumis melo var. makuwa])

HSP 1 Score: 683.7 bits (1763), Expect = 7.6e-193
Identity = 322/324 (99.38%), Postives = 324/324 (100.00%), Query Frame = 0

Query: 1   MQGGAERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISMVVLLQ 60
           MQGGAERDDGVMATDFFWSYTDEPHASRRRQILS+YPQIRELFGPDPWAFFKISMVVLLQ
Sbjct: 1   MQGGAERDDGVMATDFFWSYTDEPHASRRRQILSKYPQIRELFGPDPWAFFKISMVVLLQ 60

Query: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120
           LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGIDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDG+DMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMDKTVGPFSRMKRMPRKSE
Sbjct: 301 IYMYIMDKTVGPFSRMKRMPRKSE 324

BLAST of Cmc12g0331211 vs. NCBI nr
Match: XP_004146595.1 (sphingolipid delta(4)-desaturase DES1-like [Cucumis sativus] >KGN64668.1 hypothetical protein Csa_014335 [Cucumis sativus])

HSP 1 Score: 679.5 bits (1752), Expect = 1.4e-191
Identity = 320/324 (98.77%), Postives = 322/324 (99.38%), Query Frame = 0

Query: 1   MQGGAERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISMVVLLQ 60
           M GG ERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKIS+VVLLQ
Sbjct: 1   MPGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60

Query: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120
           LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGIDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDG+DMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMDKTVGPFSRMKRMPRKSE
Sbjct: 301 IYMYIMDKTVGPFSRMKRMPRKSE 324

BLAST of Cmc12g0331211 vs. NCBI nr
Match: XP_038893930.1 (sphingolipid delta(4)-desaturase DES1-like [Benincasa hispida])

HSP 1 Score: 669.5 bits (1726), Expect = 1.5e-188
Identity = 314/324 (96.91%), Postives = 320/324 (98.77%), Query Frame = 0

Query: 1   MQGGAERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISMVVLLQ 60
           MQGG ERD+GVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKIS+VVLLQ
Sbjct: 1   MQGGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60

Query: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120
           LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYN+WLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNRWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGIDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDG+DMDVPS TEAHLVTNVVSKAIWV+FQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSLTEAHLVTNVVSKAIWVVFQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPG WEFINFIIQIALDVAMVY WSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGQWEFINFIIQIALDVAMVYLWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMD+TVGPFSRMKRMPRKSE
Sbjct: 301 IYMYIMDRTVGPFSRMKRMPRKSE 324

BLAST of Cmc12g0331211 vs. NCBI nr
Match: KAG7012427.1 (Sphingolipid delta(4)-desaturase DES1-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 651.4 bits (1679), Expect = 4.2e-183
Identity = 302/324 (93.21%), Postives = 315/324 (97.22%), Query Frame = 0

Query: 1   MQGGAERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISMVVLLQ 60
           M  G ERD+GVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKI++VVLLQ
Sbjct: 1   MPRGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKIAVVVLLQ 60

Query: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120
           LWTATTLHSADWLKILA+AYFFGSFLNHNLFLAIHELSHNLAFSTPD+N+WLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAVAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGIDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDG+DMDVPS TEA LVTN+VSKAIWV+FQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSMTEARLVTNIVSKAIWVVFQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPGHWEFINFIIQI LD  MVYFWSWK+FAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGHWEFINFIIQIGLDFTMVYFWSWKTFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPE+YNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEFYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMD+TVGPFSRMKRM +KSE
Sbjct: 301 IYMYIMDRTVGPFSRMKRMAKKSE 324

BLAST of Cmc12g0331211 vs. NCBI nr
Match: XP_022955306.1 (sphingolipid delta(4)-desaturase DES1-like [Cucurbita moschata] >KAG6573258.1 Sphingolipid delta(4)-desaturase DES1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 651.0 bits (1678), Expect = 5.5e-183
Identity = 302/324 (93.21%), Postives = 315/324 (97.22%), Query Frame = 0

Query: 1   MQGGAERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISMVVLLQ 60
           M  G ERD+GVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKI++VVLLQ
Sbjct: 1   MPRGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKIAVVVLLQ 60

Query: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120
           LWTATTLHSADWLKILA+AYFFGSFLNHNLFLAIHELSHNLAFSTPD+N+WLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAVAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGIDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDG+DMDVPS TEA LVTN+VSKAIWV+FQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSTTEARLVTNIVSKAIWVVFQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPGHWEFINFIIQI LD  MVYFWSWK+FAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGHWEFINFIIQIGLDFTMVYFWSWKTFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPE+YNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEFYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMD+TVGPFSRMKRM +KSE
Sbjct: 301 IYMYIMDRTVGPFSRMKRMAKKSE 324

BLAST of Cmc12g0331211 vs. ExPASy Swiss-Prot
Match: Q6H5U3 (Sphingolipid delta(4)-desaturase DES1-like OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0639600 PE=2 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 1.0e-163
Identity = 264/322 (81.99%), Postives = 290/322 (90.06%), Query Frame = 0

Query: 3   GGAERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISMVVLLQLW 62
           G    ++GVMATDFFWSYTDEPHA+RRR+IL+++PQI+ELFGPDP AF KI+ VV LQLW
Sbjct: 6   GDGREEEGVMATDFFWSYTDEPHATRRREILAKHPQIKELFGPDPLAFLKIAAVVSLQLW 65

Query: 63  TATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPIGV 122
           TAT L  A W+KIL +AYFFGSFLNHNLFLAIHELSHNLAF+TP YN+WLGIFANLPIGV
Sbjct: 66  TATLLRDASWVKILTVAYFFGSFLNHNLFLAIHELSHNLAFTTPSYNRWLGIFANLPIGV 125

Query: 123 PMSITFQKYHLEHHRFQGVDGIDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLK 182
           PMSITFQKYHLEHHRFQGVDGIDMD+PSQ EAH V N +SK++WV+FQLFFYALRPLFLK
Sbjct: 126 PMSITFQKYHLEHHRFQGVDGIDMDIPSQAEAHAVKNTLSKSVWVVFQLFFYALRPLFLK 185

Query: 183 PKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNP 242
           PKPPG WEF N IIQIALD +MVYF+ WKS AYLILSTFVGGGMHPMAGHFISEHYVFNP
Sbjct: 186 PKPPGLWEFTNLIIQIALDASMVYFFGWKSLAYLILSTFVGGGMHPMAGHFISEHYVFNP 245

Query: 243 KQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIY 302
            QETYSYYGPLNL+TW VGYHNEHHDFPRIPG++LYKV+EIAPEYYN L SYKSWSQVIY
Sbjct: 246 DQETYSYYGPLNLMTWHVGYHNEHHDFPRIPGTRLYKVREIAPEYYNNLKSYKSWSQVIY 305

Query: 303 MYIMDKTVGPFSRMKRMPRKSE 325
           MYIMD+TVGPFSRMKR   K +
Sbjct: 306 MYIMDQTVGPFSRMKRKAPKKD 327

BLAST of Cmc12g0331211 vs. ExPASy Swiss-Prot
Match: Q9ZPH4 (Sphingolipid delta(4)-desaturase DES1-like OS=Arabidopsis thaliana OX=3702 GN=At4g04930 PE=2 SV=1)

HSP 1 Score: 572.4 bits (1474), Expect = 3.3e-162
Identity = 266/319 (83.39%), Postives = 292/319 (91.54%), Query Frame = 0

Query: 6   ERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISMVVLLQLWTAT 65
           E  +GVMATDFFWSYTDEPHASRRRQILS YPQIR+LFGPDPWAF KI++VV+LQL TA 
Sbjct: 14  EEREGVMATDFFWSYTDEPHASRRRQILSCYPQIRQLFGPDPWAFLKITLVVILQLSTAA 73

Query: 66  TLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPIGVPMS 125
            LH++ WLKIL+IAYFFGSFLNHNLFLAIHELSHNLAFSTP YN+ LGIFANLPIGVPMS
Sbjct: 74  ILHNSGWLKILSIAYFFGSFLNHNLFLAIHELSHNLAFSTPVYNRCLGIFANLPIGVPMS 133

Query: 126 ITFQKYHLEHHRFQGVDGIDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLKPKP 185
           +TFQKYHLEHHRFQGVDGIDMDVP+ TEAHLVTN+ +K IWV  QLFFYALRP+F+KPKP
Sbjct: 134 VTFQKYHLEHHRFQGVDGIDMDVPTYTEAHLVTNIFAKTIWVFLQLFFYALRPIFIKPKP 193

Query: 186 PGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQE 245
           PG+WEFINF+IQI LDV++V F+ W+SFAYLILSTFVGGGMHPMAGHFISEHYVFNP QE
Sbjct: 194 PGYWEFINFLIQIVLDVSVVLFFGWRSFAYLILSTFVGGGMHPMAGHFISEHYVFNPNQE 253

Query: 246 TYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIYMYI 305
           TYSYYGPLNLLTWSVGYHNEHHDFPRIPG+KL+ VKEIA EYY  L+SYKSWSQVIYMYI
Sbjct: 254 TYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLHLVKEIAGEYYEGLESYKSWSQVIYMYI 313

Query: 306 MDKTVGPFSRMKRMPRKSE 325
           MD TVGP+SRMKR   KS+
Sbjct: 314 MDTTVGPYSRMKRKLSKSD 332

BLAST of Cmc12g0331211 vs. ExPASy Swiss-Prot
Match: Q6UQ04 (Sphingolipid delta(4)-desaturase DES1 OS=Microtus fortis OX=100897 GN=DEGS1 PE=2 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 1.1e-96
Identity = 160/316 (50.63%), Postives = 222/316 (70.25%), Query Frame = 0

Query: 11  VMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISMVVLLQLWTATTLHSA 70
           V   +F W YTD+PH  RRR+IL++YP+I+ L  PDP   + I+ + L+QL +   +   
Sbjct: 5   VSREEFEWVYTDQPHTDRRREILAKYPEIKSLMKPDPNLIWIITAMFLVQLISLYLVKDL 64

Query: 71  DWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFS--TPDYNKWLGIFANLPIGVPMSITF 130
           DW  ++  +Y FGS LNH++ LAIHE+SHN  F      +N+W GIFANLPIGVP S++F
Sbjct: 65  DWKWVIFWSYVFGSCLNHSMTLAIHEISHNFPFGHHKAVWNRWFGIFANLPIGVPYSVSF 124

Query: 131 QKYHLEHHRFQGVDGIDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLKPKPPGH 190
           ++YH++HHR+ G DGID+D+P+  E         K +WVI Q  FYALRPLF+ PKP  +
Sbjct: 125 KRYHMDHHRYLGADGIDVDIPTDFEGWFFCTTFRKLVWVILQPLFYALRPLFINPKPITY 184

Query: 191 WEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYS 250
            E IN + QI LD+ + Y +  KS  Y++ ++ +G G+HP++GHFI+EHY+F    ETYS
Sbjct: 185 LEIINTVTQIILDIIVYYVFGIKSLVYMLAASLLGLGLHPISGHFIAEHYMFLKGHETYS 244

Query: 251 YYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIYMYIMDK 310
           YYGPLNLLT++VGYHNEHHDFP +PG  L  V++IA EYY++L  Y SW +V+Y ++MD 
Sbjct: 245 YYGPLNLLTFNVGYHNEHHDFPNVPGKNLPLVRKIAAEYYDKLPQYNSWIKVLYDFVMDD 304

Query: 311 TVGPFSRMKRMPRKSE 325
           T+ P+SRMKR P+ SE
Sbjct: 305 TISPYSRMKRPPKGSE 320

BLAST of Cmc12g0331211 vs. ExPASy Swiss-Prot
Match: O15121 (Sphingolipid delta(4)-desaturase DES1 OS=Homo sapiens OX=9606 GN=DEGS1 PE=1 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 1.5e-95
Identity = 159/310 (51.29%), Postives = 216/310 (69.68%), Query Frame = 0

Query: 11  VMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISMVVLLQLWTATTLHSA 70
           V   DF W YTD+PHA RRR+IL++YP+I+ L  PDP   + I M+VL QL     +   
Sbjct: 5   VSREDFEWVYTDQPHADRRREILAKYPEIKSLMKPDPNLIWIIIMMVLTQLGAFYIVKDL 64

Query: 71  DWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFST--PDYNKWLGIFANLPIGVPMSITF 130
           DW  ++  AY FGS +NH++ LAIHE++HN AF      +N+W G+FANLPIG+P SI+F
Sbjct: 65  DWKWVIFGAYAFGSCINHSMTLAIHEIAHNAAFGNCKAMWNRWFGMFANLPIGIPYSISF 124

Query: 131 QKYHLEHHRFQGVDGIDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLKPKPPGH 190
           ++YH++HHR+ G DG+D+D+P+  E         K IWVI Q  FYA RPLF+ PKP  +
Sbjct: 125 KRYHMDHHRYLGADGVDVDIPTDFEGWFFCTAFRKFIWVILQPLFYAFRPLFINPKPITY 184

Query: 191 WEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYS 250
            E IN + Q+  D+ + YF   KS  Y++ ++ +G G+HP++GHFI+EHY+F    ETYS
Sbjct: 185 LEVINTVAQVTFDILIYYFLGIKSLVYMLAASLLGLGLHPISGHFIAEHYMFLKGHETYS 244

Query: 251 YYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIYMYIMDK 310
           YYGPLNLLT++VGYHNEHHDFP IPG  L  V++IA EYY+ L  Y SW +V+Y ++MD 
Sbjct: 245 YYGPLNLLTFNVGYHNEHHDFPNIPGKSLPLVRKIAAEYYDNLPHYNSWIKVLYDFVMDD 304

Query: 311 TVGPFSRMKR 319
           T+ P+SRMKR
Sbjct: 305 TISPYSRMKR 314

BLAST of Cmc12g0331211 vs. ExPASy Swiss-Prot
Match: Q5XIF5 (Sphingolipid delta(4)-desaturase DES1 OS=Rattus norvegicus OX=10116 GN=Degs1 PE=2 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 3.4e-95
Identity = 156/316 (49.37%), Postives = 223/316 (70.57%), Query Frame = 0

Query: 11  VMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISMVVLLQLWTATTLHSA 70
           V   +F W YTD+PHA+RR++IL++YP+I+ L  PDP   + ++ ++L+QL +   +   
Sbjct: 5   VSREEFEWVYTDQPHAARRQEILAKYPEIKSLMKPDPNLIWIVTSMLLVQLASFYLVKDL 64

Query: 71  DWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFS--TPDYNKWLGIFANLPIGVPMSITF 130
           DW  ++  +Y FGS LNH++ LAIHE+SHN  F      +N+W G+FANL +GVP SI+F
Sbjct: 65  DWKWLMFWSYAFGSCLNHSMTLAIHEISHNFPFGHHKAMWNRWFGMFANLSLGVPYSISF 124

Query: 131 QKYHLEHHRFQGVDGIDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLKPKPPGH 190
           ++YH++HHR+ G DGID+D+P+  E       + K +WVI Q  FYA RPLF+ PKP  H
Sbjct: 125 KRYHMDHHRYLGADGIDVDIPTDFEGWFFCTTLRKLVWVILQPLFYAFRPLFINPKPITH 184

Query: 191 WEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYS 250
            E IN +IQ+  DV + Y +  KS  Y++ ++ +G G+HP++GHFI+EHY+F    ETYS
Sbjct: 185 LEVINTVIQVTFDVLVYYVFGVKSLVYMLAASLLGLGLHPISGHFIAEHYMFLKGHETYS 244

Query: 251 YYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIYMYIMDK 310
           YYGPLNLLT++VGYHNEHHDFP +PG  L  V++IA EYY+ L  Y SW +V+Y ++MD 
Sbjct: 245 YYGPLNLLTFNVGYHNEHHDFPNVPGKNLPLVRKIASEYYDNLPHYNSWIRVLYDFVMDD 304

Query: 311 TVGPFSRMKRMPRKSE 325
           T+ P+SRMKR P+ +E
Sbjct: 305 TISPYSRMKRPPKGNE 320

BLAST of Cmc12g0331211 vs. ExPASy TrEMBL
Match: A0A5D3DSS5 (Sphingolipid delta(4)-desaturase DES1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00680 PE=3 SV=1)

HSP 1 Score: 683.7 bits (1763), Expect = 3.7e-193
Identity = 322/324 (99.38%), Postives = 324/324 (100.00%), Query Frame = 0

Query: 1   MQGGAERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISMVVLLQ 60
           MQGGAERDDGVMATDFFWSYTDEPHASRRRQILS+YPQIRELFGPDPWAFFKISMVVLLQ
Sbjct: 1   MQGGAERDDGVMATDFFWSYTDEPHASRRRQILSKYPQIRELFGPDPWAFFKISMVVLLQ 60

Query: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120
           LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGIDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDG+DMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMDKTVGPFSRMKRMPRKSE
Sbjct: 301 IYMYIMDKTVGPFSRMKRMPRKSE 324

BLAST of Cmc12g0331211 vs. ExPASy TrEMBL
Match: A0A1S3B5L3 (Sphingolipid delta(4)-desaturase DES1-like OS=Cucumis melo OX=3656 GN=LOC103486276 PE=3 SV=1)

HSP 1 Score: 683.7 bits (1763), Expect = 3.7e-193
Identity = 322/324 (99.38%), Postives = 324/324 (100.00%), Query Frame = 0

Query: 1   MQGGAERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISMVVLLQ 60
           MQGGAERDDGVMATDFFWSYTDEPHASRRRQILS+YPQIRELFGPDPWAFFKISMVVLLQ
Sbjct: 1   MQGGAERDDGVMATDFFWSYTDEPHASRRRQILSKYPQIRELFGPDPWAFFKISMVVLLQ 60

Query: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120
           LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGIDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDG+DMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMDKTVGPFSRMKRMPRKSE
Sbjct: 301 IYMYIMDKTVGPFSRMKRMPRKSE 324

BLAST of Cmc12g0331211 vs. ExPASy TrEMBL
Match: A0A0A0LSB9 (Sphingolipid delta(4)-desaturase DES1-like OS=Cucumis sativus OX=3659 GN=Csa_1G073750 PE=3 SV=1)

HSP 1 Score: 679.5 bits (1752), Expect = 7.0e-192
Identity = 320/324 (98.77%), Postives = 322/324 (99.38%), Query Frame = 0

Query: 1   MQGGAERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISMVVLLQ 60
           M GG ERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKIS+VVLLQ
Sbjct: 1   MPGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60

Query: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120
           LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGIDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDG+DMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMDKTVGPFSRMKRMPRKSE
Sbjct: 301 IYMYIMDKTVGPFSRMKRMPRKSE 324

BLAST of Cmc12g0331211 vs. ExPASy TrEMBL
Match: A0A6J1GTF2 (Sphingolipid delta(4)-desaturase DES1-like OS=Cucurbita moschata OX=3662 GN=LOC111457308 PE=3 SV=1)

HSP 1 Score: 651.0 bits (1678), Expect = 2.7e-183
Identity = 302/324 (93.21%), Postives = 315/324 (97.22%), Query Frame = 0

Query: 1   MQGGAERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISMVVLLQ 60
           M  G ERD+GVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKI++VVLLQ
Sbjct: 1   MPRGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKIAVVVLLQ 60

Query: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120
           LWTATTLHSADWLKILA+AYFFGSFLNHNLFLAIHELSHNLAFSTPD+N+WLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAVAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGIDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDG+DMDVPS TEA LVTN+VSKAIWV+FQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSTTEARLVTNIVSKAIWVVFQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPGHWEFINFIIQI LD  MVYFWSWK+FAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGHWEFINFIIQIGLDFTMVYFWSWKTFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPE+YNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEFYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMD+TVGPFSRMKRM +KSE
Sbjct: 301 IYMYIMDRTVGPFSRMKRMAKKSE 324

BLAST of Cmc12g0331211 vs. ExPASy TrEMBL
Match: A0A6J1EFX3 (Sphingolipid delta(4)-desaturase DES1-like OS=Cucurbita moschata OX=3662 GN=LOC111433967 PE=3 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 7.7e-183
Identity = 301/324 (92.90%), Postives = 316/324 (97.53%), Query Frame = 0

Query: 1   MQGGAERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISMVVLLQ 60
           M+GG ERD+GVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKIS+VVLLQ
Sbjct: 1   MRGGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60

Query: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120
           LWTATTLH+A+WLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPD+N+WLGIFANLPI
Sbjct: 61  LWTATTLHNAEWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGIDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDG+DMDVPSQTEA LVTNV+SKAIWV+ QLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEARLVTNVISKAIWVVLQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPG WEFINF IQI LDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGRWEFINFFIQITLDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPG+KLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMD++VGPFSRMKR+ +K E
Sbjct: 301 IYMYIMDRSVGPFSRMKRLSKKPE 324

BLAST of Cmc12g0331211 vs. TAIR 10
Match: AT4G04930.1 (fatty acid desaturase family protein )

HSP 1 Score: 572.4 bits (1474), Expect = 2.3e-163
Identity = 266/319 (83.39%), Postives = 292/319 (91.54%), Query Frame = 0

Query: 6   ERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISMVVLLQLWTAT 65
           E  +GVMATDFFWSYTDEPHASRRRQILS YPQIR+LFGPDPWAF KI++VV+LQL TA 
Sbjct: 14  EEREGVMATDFFWSYTDEPHASRRRQILSCYPQIRQLFGPDPWAFLKITLVVILQLSTAA 73

Query: 66  TLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPIGVPMS 125
            LH++ WLKIL+IAYFFGSFLNHNLFLAIHELSHNLAFSTP YN+ LGIFANLPIGVPMS
Sbjct: 74  ILHNSGWLKILSIAYFFGSFLNHNLFLAIHELSHNLAFSTPVYNRCLGIFANLPIGVPMS 133

Query: 126 ITFQKYHLEHHRFQGVDGIDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLKPKP 185
           +TFQKYHLEHHRFQGVDGIDMDVP+ TEAHLVTN+ +K IWV  QLFFYALRP+F+KPKP
Sbjct: 134 VTFQKYHLEHHRFQGVDGIDMDVPTYTEAHLVTNIFAKTIWVFLQLFFYALRPIFIKPKP 193

Query: 186 PGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQE 245
           PG+WEFINF+IQI LDV++V F+ W+SFAYLILSTFVGGGMHPMAGHFISEHYVFNP QE
Sbjct: 194 PGYWEFINFLIQIVLDVSVVLFFGWRSFAYLILSTFVGGGMHPMAGHFISEHYVFNPNQE 253

Query: 246 TYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIYMYI 305
           TYSYYGPLNLLTWSVGYHNEHHDFPRIPG+KL+ VKEIA EYY  L+SYKSWSQVIYMYI
Sbjct: 254 TYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLHLVKEIAGEYYEGLESYKSWSQVIYMYI 313

Query: 306 MDKTVGPFSRMKRMPRKSE 325
           MD TVGP+SRMKR   KS+
Sbjct: 314 MDTTVGPYSRMKRKLSKSD 332

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008442400.17.6e-19399.38PREDICTED: sphingolipid delta(4)-desaturase DES1-like [Cucumis melo] >KAA0056978... [more]
XP_004146595.11.4e-19198.77sphingolipid delta(4)-desaturase DES1-like [Cucumis sativus] >KGN64668.1 hypothe... [more]
XP_038893930.11.5e-18896.91sphingolipid delta(4)-desaturase DES1-like [Benincasa hispida][more]
KAG7012427.14.2e-18393.21Sphingolipid delta(4)-desaturase DES1-like protein [Cucurbita argyrosperma subsp... [more]
XP_022955306.15.5e-18393.21sphingolipid delta(4)-desaturase DES1-like [Cucurbita moschata] >KAG6573258.1 Sp... [more]
Match NameE-valueIdentityDescription
Q6H5U31.0e-16381.99Sphingolipid delta(4)-desaturase DES1-like OS=Oryza sativa subsp. japonica OX=39... [more]
Q9ZPH43.3e-16283.39Sphingolipid delta(4)-desaturase DES1-like OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Q6UQ041.1e-9650.63Sphingolipid delta(4)-desaturase DES1 OS=Microtus fortis OX=100897 GN=DEGS1 PE=2... [more]
O151211.5e-9551.29Sphingolipid delta(4)-desaturase DES1 OS=Homo sapiens OX=9606 GN=DEGS1 PE=1 SV=1[more]
Q5XIF53.4e-9549.37Sphingolipid delta(4)-desaturase DES1 OS=Rattus norvegicus OX=10116 GN=Degs1 PE=... [more]
Match NameE-valueIdentityDescription
A0A5D3DSS53.7e-19399.38Sphingolipid delta(4)-desaturase DES1-like OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3B5L33.7e-19399.38Sphingolipid delta(4)-desaturase DES1-like OS=Cucumis melo OX=3656 GN=LOC1034862... [more]
A0A0A0LSB97.0e-19298.77Sphingolipid delta(4)-desaturase DES1-like OS=Cucumis sativus OX=3659 GN=Csa_1G0... [more]
A0A6J1GTF22.7e-18393.21Sphingolipid delta(4)-desaturase DES1-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1EFX37.7e-18392.90Sphingolipid delta(4)-desaturase DES1-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT4G04930.12.3e-16383.39fatty acid desaturase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013866Sphingolipid delta4-desaturase, N-terminalSMARTSM01269Lipid_DES_2coord: 11..49
e-value: 7.2E-21
score: 85.3
IPR013866Sphingolipid delta4-desaturase, N-terminalPFAMPF08557Lipid_DEScoord: 14..48
e-value: 6.7E-20
score: 70.3
IPR011388Sphingolipid delta4-desaturasePIRSFPIRSF017228Sphnglp_dlt4_descoord: 4..324
e-value: 2.6E-149
score: 494.8
IPR011388Sphingolipid delta4-desaturaseCDDcd03508Delta4-sphingolipid-FADS-likecoord: 32..318
e-value: 1.19327E-170
score: 473.285
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 72..290
e-value: 2.2E-20
score: 73.6
NoneNo IPR availablePANTHERPTHR12879:SF17SPHINGOLIPID DELTA(4)-DESATURASE DES1-LIKEcoord: 7..322
NoneNo IPR availablePANTHERPTHR12879SPHINGOLIPID DELTA 4 DESATURASE/C-4 HYDROXYLASE PROTEIN DES2coord: 7..322

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc12g0331211.1Cmc12g0331211.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030148 sphingolipid biosynthetic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016020 membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0042284 sphingolipid delta-4 desaturase activity