Cmc12g0328561 (gene) Melon (Charmono) v1.1

Overview


Name	Cmc12g0328561
Type	gene
Organism	Cucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Description	glucan endo-1,3-beta-glucosidase 13
Location	CMiso1.1chr12: 19716410 .. 19718385 (-)
RNA-Seq Expression	Cmc12g0328561
Synteny	Cmc12g0328561

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAGAAGATAATTTCATTTCAAGAAACTAATTATTCTTCACAAAAACCCTTTCATTTATACCTCTCTCCTTCTTCTACACCCTCCCAACTCCACCCCAACAACACACTTCATTTTTTTTTTTAACCATAAAAATGAAAACCAAAATCTGGGTTTTGGAGATCCTCCTCTTTCTACATTATTTCTTTCTGGGTTTGTTTTATTTCTCACAAAAACTTCATCTTTTATCCCATTAACAAACCAGAAACAAAAAATACAGTACTTTATTACTTACTTTGTTTTACTTAACTTCTTCATGTTGTGGTTTTGTTAGGGAGAGCAATGGCAGCGGAGGCGGGGATGCCGGTGATGGCGGCGCCGGTGGAAGGGAACACGACGTTCATAGATGGGACTACGTGGTGTGTGGCGAAGCCTGGAGTTTCACAGATTGATTTGCAAAATGCTTTGGATTGGGCTTGTGGAATGGGGAAAGCTGATTGTAGAGCGATTCAAAAGGGAGGGCGTTGTTATGAACCGGATACCCTCTTGTCCCATGCGTCTTATGCATTTAATAGCTATTATCAACAAAATGGCAACTCTGATATTGCTTGCAATTTTGGTGGTTGTGCTACTTTGACTAAGAAAGACCCAAGTAAAATCTCTCCCTCTCTCTCTATTTCTCTTCGATGACTTTCAAATGATGTCCATGGATTCGAACATCTGTCTTTATGGTCGAAGTACATAGTTTTATGAGTTGATGAATGGGTGCTTTTTGTCTACCATTGTTGAGATTGTATTTGTTAATTGTTTGGTTTTTTTGAAAGTTTGTTTATTTTGGTTTAGATTATGTTTGAGGTTGATTTGGTTTTTTGTTTTTTATTTTGAACTTGTATCTATTGAATCATAGTTCTTTTGCTTCTTATCTACTTTTATTAATGTTTCAAAAATTAGACTGAAGTTTAAAAATTTTAAAAAGTAATTTTAAAATATTTTTTTTGTTCTTTCAAATTTGGTTAAGAACGGAACTATTTTTCTAAATAAAAATAAAATGTAAACTACTCTAAGAAAATGTGAATAAATAGACTTAACCAAAATCTAAAAAGAAAAAGAAAAAAACAAGATGACTATAAAAGGAGTTAAAGATACGTTTTTGTTAATAACTTATGGTTGGACCTGAAAACTTAGAGAAAGGGATAAGTAGCTGTAATCAATATGTAGAGTATTGAATTAATAATTTTATAAGATTAATTTTCATAAATTAAATATAGGTTATAAAAATCCATGTTGTTAACAAACATTTATACTTAAAAATGTAACAAACCATTCCTCATCAAATATTTATCTTCTTCTTTTGACAGGTTATGGGAAATGTGATTATTCAGCATCCAGGTATTTTTATTTATTTCACAAGCAATTTGACAATAATAATACCCACCCATAATTAAATAAATAAATAAATAATAGTAATAATAATATTGATTTTGATTATTTTGTTTGGAAAATGACACCCAGTTCGACAAACTCGGCACCTTCAAAATCCAAGAACAGTCCAAGCCATATCTGCCTGAAGTTGCTTTCTGGGGTTTTTGTGGTTCTAAATCTATATTTTGGAAGCTAATGAATTATTTGATTTTGGATTAAGAGCTTAGTTAATTAATTTTAATTAACAAAAGCCAAAGTTGAATCTGAAGGAAAGGAGACAAAAAGAAAAGGCAAAGAATATTGGATGAGTTAATCAATTAATTAACATTCTGTAAATTGTTATGATTTTTGCTTAGTTTTTATTCTTCTCTTTGTCGATGGTTTAGGAATCTTAGTTTCATTTTTCAAGATTATTCTCAATTTATTCTTACTTTCTTTTGTATAATCCTTGCCTTTATTTATTATTATTAATATCTTTTTTTTGGGTTTTCCTTTATCCATTATTCAGAAAAAAAGGAATTGTTTCCTTAACGATGGAGGCAAGTAATTGTAATTTTTATTTTTAGTCACTGCGTT

mRNA sequence

AAAAGAAGATAATTTCATTTCAAGAAACTAATTATTCTTCACAAAAACCCTTTCATTTATACCTCTCTCCTTCTTCTACACCCTCCCAACTCCACCCCAACAACACACTTCATTTTTTTTTTTAACCATAAAAATGAAAACCAAAATCTGGGTTTTGGAGATCCTCCTCTTTCTACATTATTTCTTTCTGGGGAGAGCAATGGCAGCGGAGGCGGGGATGCCGGTGATGGCGGCGCCGGTGGAAGGGAACACGACGTTCATAGATGGGACTACGTGGTGTGTGGCGAAGCCTGGAGTTTCACAGATTGATTTGCAAAATGCTTTGGATTGGGCTTGTGGAATGGGGAAAGCTGATTGTAGAGCGATTCAAAAGGGAGGGCGTTGTTATGAACCGGATACCCTCTTGTCCCATGCGTCTTATGCATTTAATAGCTATTATCAACAAAATGGCAACTCTGATATTGCTTGCAATTTTGGTGGTTGTGCTACTTTGACTAAGAAAGACCCAAGTTATGGGAAATGTGATTATTCAGCATCCAGTTCGACAAACTCGGCACCTTCAAAATCCAAGAACAGTCCAAGCCATATCTGCCTGAAGTTGCTTTCTGGGGTTTTTGTGGTTCTAAATCTATATTTTGGAAGCTAATGAATTATTTGATTTTGGATTAAGAGCTTAGTTAATTAATTTTAATTAACAAAAGCCAAAGTTGAATCTGAAGGAAAGGAGACAAAAAGAAAAGGCAAAGAATATTGGATGAGTTAATCAATTAATTAACATTCTGTAAATTGTTATGATTTTTGCTTAGTTTTTATTCTTCTCTTTGTCGATGGTTTAGGAATCTTAGTTTCATTTTTCAAGATTATTCTCAATTTATTCTTACTTTCTTTTGTATAATCCTTGCCTTTATTTATTATTATTAATATCTTTTTTTTGGGTTTTCCTTTATCCATTATTCAGAAAAAAAGGAATTGTTTCCTTAACGATGGAGGCAAGTAATTGTAATTTTTATTTTTAGTCACTGCGTT

Coding sequence (CDS)

ATGAAAACCAAAATCTGGGTTTTGGAGATCCTCCTCTTTCTACATTATTTCTTTCTGGGGAGAGCAATGGCAGCGGAGGCGGGGATGCCGGTGATGGCGGCGCCGGTGGAAGGGAACACGACGTTCATAGATGGGACTACGTGGTGTGTGGCGAAGCCTGGAGTTTCACAGATTGATTTGCAAAATGCTTTGGATTGGGCTTGTGGAATGGGGAAAGCTGATTGTAGAGCGATTCAAAAGGGAGGGCGTTGTTATGAACCGGATACCCTCTTGTCCCATGCGTCTTATGCATTTAATAGCTATTATCAACAAAATGGCAACTCTGATATTGCTTGCAATTTTGGTGGTTGTGCTACTTTGACTAAGAAAGACCCAAGTTATGGGAAATGTGATTATTCAGCATCCAGTTCGACAAACTCGGCACCTTCAAAATCCAAGAACAGTCCAAGCCATATCTGCCTGAAGTTGCTTTCTGGGGTTTTTGTGGTTCTAAATCTATATTTTGGAAGCTAA

Protein sequence

MKTKIWVLEILLFLHYFFLGRAMAAEAGMPVMAAPVEGNTTFIDGTTWCVAKPGVSQIDLQNALDWACGMGKADCRAIQKGGRCYEPDTLLSHASYAFNSYYQQNGNSDIACNFGGCATLTKKDPSYGKCDYSASSSTNSAPSKSKNSPSHICLKLLSGVFVVLNLYFGS

Homology

[Close]

BLAST of Cmc12g0328561 vs. NCBI nr
Match: XP_008444815.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 13 [Cucumis melo])

HSP 1 Score: 349.0 bits (894), Expect = 2.3e-92
Identity = 169/170 (99.41%), Postives = 169/170 (99.41%), Query Frame = 0


Query: 1   MKTKIWVLEILLFLHYFFLGRAMAAEAGMPVMAAPVEGNTTFIDGTTWCVAKPGVSQIDL 60
           MKTKIWVLEILLFLHYFFLG AMAAEAGMPVMAAPVEGNTTFIDGTTWCVAKPGVSQIDL
Sbjct: 1   MKTKIWVLEILLFLHYFFLGGAMAAEAGMPVMAAPVEGNTTFIDGTTWCVAKPGVSQIDL 60

Query: 61  QNALDWACGMGKADCRAIQKGGRCYEPDTLLSHASYAFNSYYQQNGNSDIACNFGGCATL 120
           QNALDWACGMGKADCRAIQKGGRCYEPDTLLSHASYAFNSYYQQNGNSDIACNFGGCATL
Sbjct: 61  QNALDWACGMGKADCRAIQKGGRCYEPDTLLSHASYAFNSYYQQNGNSDIACNFGGCATL 120

Query: 121 TKKDPSYGKCDYSASSSTNSAPSKSKNSPSHICLKLLSGVFVVLNLYFGS 171
           TKKDPSYGKCDYSASSSTNSAPSKSKNSPSHICLKLLSGVFVVLNLYFGS
Sbjct: 121 TKKDPSYGKCDYSASSSTNSAPSKSKNSPSHICLKLLSGVFVVLNLYFGS 170

[Close]

BLAST of Cmc12g0328561 vs. NCBI nr
Match: XP_004135538.1 (major pollen allergen Ole e 10 [Cucumis sativus] >KAE8653300.1 hypothetical protein Csa_023370 [Cucumis sativus])

HSP 1 Score: 329.3 bits (843), Expect = 1.9e-86
Identity = 162/171 (94.74%), Postives = 165/171 (96.49%), Query Frame = 0


Query: 1   MKTKIWVLEI-LLFLHYFFLGRAMAAEAGMPVMAAPVEGNTTFIDGTTWCVAKPGVSQID 60
           MKTKIWVLEI LLFLHYFFLG AMAAEAGMPVMAAP EGNTTFIDGTTWCVAK GVSQID
Sbjct: 1   MKTKIWVLEILLLFLHYFFLGGAMAAEAGMPVMAAPAEGNTTFIDGTTWCVAKAGVSQID 60

Query: 61  LQNALDWACGMGKADCRAIQKGGRCYEPDTLLSHASYAFNSYYQQNGNSDIACNFGGCAT 120
           LQNALDWACGMGKADCRAIQKGGRCYEPDTLLSHAS+AFNSYYQQNGNSDIACNFGGCAT
Sbjct: 61  LQNALDWACGMGKADCRAIQKGGRCYEPDTLLSHASFAFNSYYQQNGNSDIACNFGGCAT 120

Query: 121 LTKKDPSYGKCDYSASSSTNSAPSKSKNSPSHICLKLLSGVFVVLNLYFGS 171
           LTKKDPSYGKCDYSASSSTNSAPSKSKNSP+HICLKL+S V VVLNLYFGS
Sbjct: 121 LTKKDPSYGKCDYSASSSTNSAPSKSKNSPTHICLKLVSVVSVVLNLYFGS 171

[Close]

BLAST of Cmc12g0328561 vs. NCBI nr
Match: XP_038895270.1 (major pollen allergen Ole e 10 [Benincasa hispida])

HSP 1 Score: 299.3 bits (765), Expect = 2.1e-77
Identity = 146/173 (84.39%), Postives = 154/173 (89.02%), Query Frame = 0


Query: 1   MKTKIWVLEILLFLHYFFLGRAMAAEAGMPVMAA---PVEGNTTFIDGTTWCVAKPGVSQ 60
           MK KIWVLEILL LHY FLG   AAEAGM V  A   P EGNTTF+DGT+WCVAK G SQ
Sbjct: 1   MKRKIWVLEILLLLHYLFLGGGTAAEAGMAVTTAAMTPPEGNTTFLDGTSWCVAKAGASQ 60

Query: 61  IDLQNALDWACGMGKADCRAIQKGGRCYEPDTLLSHASYAFNSYYQQNGNSDIACNFGGC 120
           IDLQNALDWACG+GKADCRAIQKGG CY+PDTLLSHASYAFNSYYQQNGNSDIACNFGGC
Sbjct: 61  IDLQNALDWACGLGKADCRAIQKGGPCYQPDTLLSHASYAFNSYYQQNGNSDIACNFGGC 120

Query: 121 ATLTKKDPSYGKCDYSASSSTNSAPSKSKNSPSHICLKLLSGVFVVLNLYFGS 171
           ATLTK +PSYGKCDYSASSST+SAPSK KNSPSHICLKLL GVF++LNLYFGS
Sbjct: 121 ATLTKNNPSYGKCDYSASSSTSSAPSKFKNSPSHICLKLLCGVFMLLNLYFGS 173

[Close]

BLAST of Cmc12g0328561 vs. NCBI nr
Match: XP_022140209.1 (major pollen allergen Ole e 10 [Momordica charantia])

HSP 1 Score: 259.6 bits (662), Expect = 1.9e-65
Identity = 129/179 (72.07%), Postives = 146/179 (81.56%), Query Frame = 0


Query: 1   MKTKIW----VLEILLFLHYFFLGRAMA----AEAGMPVMA-APVEGNTTFIDGTTWCVA 60
           M+TKIW    VLE +  L   FLG  MA    AEA MP    +P EGN TF+DGT+WCVA
Sbjct: 1   MRTKIWVLLLVLEAMFLLQCCFLGEGMAVEEKAEAAMPATTLSPPEGNATFLDGTSWCVA 60

Query: 61  KPGVSQIDLQNALDWACGMGKADCRAIQKGGRCYEPDTLLSHASYAFNSYYQQNGNSDIA 120
           + G SQIDLQNALDWACG+GKADCRAIQKGG CY+PDTLLSHASYAFNS+YQQNGNSDIA
Sbjct: 61  RAGASQIDLQNALDWACGLGKADCRAIQKGGACYQPDTLLSHASYAFNSFYQQNGNSDIA 120

Query: 121 CNFGGCATLTKKDPSYGKCDYSASSSTNSAPSKSKNSPSHICLKLLSGVFVVLNLYFGS 171
           CNFGG ATLTKK+PSYGKCDYSASSS NSA SK KN+ SH+CL+L++G+ +VLNLY GS
Sbjct: 121 CNFGGSATLTKKNPSYGKCDYSASSSVNSATSKLKNNGSHVCLRLVAGILMVLNLYLGS 179

[Close]

BLAST of Cmc12g0328561 vs. NCBI nr
Match: XP_022994908.1 (major pollen allergen Ole e 10-like [Cucurbita maxima])

HSP 1 Score: 259.6 bits (662), Expect = 1.9e-65
Identity = 128/178 (71.91%), Postives = 145/178 (81.46%), Query Frame = 0


Query: 1   MKTKIWVLEILLFLHYFFLGRAMA-------AEAGMPVMA-APVEGNTTFIDGTTWCVAK 60
           M  +IW L  L  L  F +G AMA       AEA MP+   +P EGNTTF+DGT+WCVAK
Sbjct: 1   MSIQIWGLVALFLLQSFLMGEAMATAVAVEKAEASMPMTTWSPPEGNTTFLDGTSWCVAK 60

Query: 61  PGVSQIDLQNALDWACGMGKADCRAIQKGGRCYEPDTLLSHASYAFNSYYQQNGNSDIAC 120
           PGVSQIDLQNALDWACG+GKADCRAIQKGGRCY+PDTL SHASYAFNSYYQQNGNSDIAC
Sbjct: 61  PGVSQIDLQNALDWACGLGKADCRAIQKGGRCYQPDTLFSHASYAFNSYYQQNGNSDIAC 120

Query: 121 NFGGCATLTKKDPSYGKCDYSASSSTNSAPSKSKNSPSHICLKLLSGVFVVLNLYFGS 171
           NFGG ATLTK+DPSYGKCDYSAS S +SAPSK K + S  CL+L+ G+++V+NLYFGS
Sbjct: 121 NFGGSATLTKRDPSYGKCDYSASRSASSAPSKWKKNGSRGCLQLVGGIWMVMNLYFGS 178

[Close]

BLAST of Cmc12g0328561 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 2.0e-25
Identity = 56/117 (47.86%), Postives = 71/117 (60.68%), Query Frame = 0


Query: 30  PVMAAPVEGNTTFIDG-------TTWCVAKPGVSQIDLQNALDWACGMGKADCRAIQKGG 89
           P  +  + GN+T   G         WC+A    S  +LQ ALDWACG G  DC A+Q   
Sbjct: 366 PSSSPIINGNSTVTIGGGGGGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQ 425

Query: 90  RCYEPDTLLSHASYAFNSYYQQNGNSDIACNFGGCATLTKKDPSYGKCDYSASSSTN 140
            C+EPDT+LSHASYAFN+YYQQ+G S I C+F G +    KDPSYG C Y  + +T+
Sbjct: 426 PCFEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDKDPSYGNCLYMIAPATD 482

[Close]

BLAST of Cmc12g0328561 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 4.4e-25
Identity = 60/142 (42.25%), Postives = 83/142 (58.45%), Query Frame = 0


Query: 38  GNTTFIDGTTWCVAKPGVSQIDLQNALDWACGMGKADCRAIQKGGRCYEPDTLLSHASYA 97
           G  +     +WC+A    S+ DL+ ALDWACG G  DC AIQ    C++PDTL+SHAS+ 
Sbjct: 359 GTNSSGSSNSWCIASSKASERDLKGALDWACGPGNVDCTAIQPSQPCFQPDTLVSHASFV 418

Query: 98  FNSYYQQNGNSDIACNFGGCATLTKKDPSYGKCDY-----------SASSSTNSAPSKSK 157
           FNSY+QQN  +D+AC+FGG      KDPSY KC Y           +A++ T+SA +   
Sbjct: 419 FNSYFQQNRATDVACSFGGAGVKVNKDPSYDKCIYITAGGNKTKATNATALTSSASTPRG 478

Query: 158 NSPSHICLKLLSGVFVVLNLYF 169
           N      LKL     ++++L+F
Sbjct: 479 NELLQWILKLC----LMISLFF 496

[Close]

BLAST of Cmc12g0328561 vs. ExPASy Swiss-Prot
Match: Q9C7U5 (Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 PE=2 SV=2)

HSP 1 Score: 108.6 bits (270), Expect = 7.0e-23
Identity = 54/114 (47.37%), Postives = 74/114 (64.91%), Query Frame = 0


Query: 47  TWCVAKPGVSQIDLQNALDWACGMGKADCRAIQKGGRCYEPDTLLSHASYAFNSYYQQNG 106
           T+C A+ G     LQ ALDWACG GK DC  I++G  CYEPD +++HA+YAF++YY Q G
Sbjct: 367 TYCTAREGADTKMLQAALDWACGPGKIDCSPIKQGETCYEPDNVVAHANYAFDTYYHQTG 426

Query: 107 NSDIACNFGGCATLTKKDPSYGKCDYSAS-------SSTN-SAPSKSKNSPSHI 153
           N+  ACNF G A++T  DPS+G C ++ S       +S N +APS +  + S I
Sbjct: 427 NNPDACNFNGVASITTTDPSHGTCVFAGSRGNGRNGTSVNITAPSANSTTSSGI 480

[Close]

BLAST of Cmc12g0328561 vs. ExPASy Swiss-Prot
Match: P52409 (Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 9.1e-23
Identity = 46/88 (52.27%), Postives = 61/88 (69.32%), Query Frame = 0


Query: 45  GTTWCVAKPGVSQIDLQNALDWACGMGKADCRAIQKGGRCYEPDTLLSHASYAFNSYYQQ 104
           G  WCVAK G +  DLQN +++ACG    DC+ IQ GG C+ P++L +HASY  N+YYQ 
Sbjct: 374 GGKWCVAKDGANGTDLQNNINYACGF--VDCKPIQSGGACFSPNSLQAHASYVMNAYYQA 433

Query: 105 NGNSDIACNFGGCATLTKKDPSYGKCDY 133
           NG++D+AC+F G   +T  DPSYG C Y
Sbjct: 434 NGHTDLACDFKGTGIVTSSDPSYGGCKY 459

[Close]

BLAST of Cmc12g0328561 vs. ExPASy Swiss-Prot
Match: Q9ZU91 (Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 PE=2 SV=2)

HSP 1 Score: 107.8 bits (268), Expect = 1.2e-22
Identity = 53/115 (46.09%), Postives = 75/115 (65.22%), Query Frame = 0


Query: 47  TWCVAKPGVSQIDLQNALDWACGMGKADCRAIQKGGRCYEPDTLLSHASYAFNSYYQQNG 106
           T+C+AK  V +  LQ ALDWACG GK DC A+ +G  CYEPD +++H++YAFN+YYQ+ G
Sbjct: 359 TFCIAKEKVDRKMLQAALDWACGPGKVDCSALMQGESCYEPDDVVAHSTYAFNAYYQKMG 418

Query: 107 NSDIACNFGGCATLTKKDPSYGKCDYSASSSTN-------SAPSKSKNSPSHICL 155
            +  +C+F G AT+T  DPS G C +  S+ +N       SA + S NS +  C+
Sbjct: 419 KASGSCDFKGVATVTTTDPSRGTCVFPGSAKSNQTLGNNTSALAPSANSTTSGCI 473

[Close]

BLAST of Cmc12g0328561 vs. ExPASy TrEMBL
Match: A0A1S3BB84 (glucan endo-1,3-beta-glucosidase 13 OS=Cucumis melo OX=3656 GN=LOC103488047 PE=4 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 1.1e-92
Identity = 169/170 (99.41%), Postives = 169/170 (99.41%), Query Frame = 0


Query: 1   MKTKIWVLEILLFLHYFFLGRAMAAEAGMPVMAAPVEGNTTFIDGTTWCVAKPGVSQIDL 60
           MKTKIWVLEILLFLHYFFLG AMAAEAGMPVMAAPVEGNTTFIDGTTWCVAKPGVSQIDL
Sbjct: 1   MKTKIWVLEILLFLHYFFLGGAMAAEAGMPVMAAPVEGNTTFIDGTTWCVAKPGVSQIDL 60

Query: 61  QNALDWACGMGKADCRAIQKGGRCYEPDTLLSHASYAFNSYYQQNGNSDIACNFGGCATL 120
           QNALDWACGMGKADCRAIQKGGRCYEPDTLLSHASYAFNSYYQQNGNSDIACNFGGCATL
Sbjct: 61  QNALDWACGMGKADCRAIQKGGRCYEPDTLLSHASYAFNSYYQQNGNSDIACNFGGCATL 120

Query: 121 TKKDPSYGKCDYSASSSTNSAPSKSKNSPSHICLKLLSGVFVVLNLYFGS 171
           TKKDPSYGKCDYSASSSTNSAPSKSKNSPSHICLKLLSGVFVVLNLYFGS
Sbjct: 121 TKKDPSYGKCDYSASSSTNSAPSKSKNSPSHICLKLLSGVFVVLNLYFGS 170

[Close]

BLAST of Cmc12g0328561 vs. ExPASy TrEMBL
Match: A0A0A0M121 (X8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G537410 PE=4 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 3.5e-78
Identity = 152/171 (88.89%), Postives = 155/171 (90.64%), Query Frame = 0


Query: 1   MKTKIWVLEI-LLFLHYFFLGRAMAAEAGMPVMAAPVEGNTTFIDGTTWCVAKPGVSQID 60
           MKTKIWVLEI LLFLHYFFLG AMAAEAGMPVMAAP EGNTTFIDGTTWCVAK GVSQID
Sbjct: 1   MKTKIWVLEILLLFLHYFFLGGAMAAEAGMPVMAAPAEGNTTFIDGTTWCVAKAGVSQID 60

Query: 61  LQNALDWACGMGKADCRAIQKGGRCYEPDTLLSHASYAFNSYYQQNGNSDIACNFGGCAT 120
           LQNALDWACGMGKADCRAIQKGGRCYEPDTLLSHAS+AFNSYYQQNGNSDIACNFGG   
Sbjct: 61  LQNALDWACGMGKADCRAIQKGGRCYEPDTLLSHASFAFNSYYQQNGNSDIACNFGG--- 120

Query: 121 LTKKDPSYGKCDYSASSSTNSAPSKSKNSPSHICLKLLSGVFVVLNLYFGS 171
                  YGKCDYSASSSTNSAPSKSKNSP+HICLKL+S V VVLNLYFGS
Sbjct: 121 -------YGKCDYSASSSTNSAPSKSKNSPTHICLKLVSVVSVVLNLYFGS 161

[Close]

BLAST of Cmc12g0328561 vs. ExPASy TrEMBL
Match: A0A6J1K6E1 (major pollen allergen Ole e 10-like OS=Cucurbita maxima OX=3661 GN=LOC111490492 PE=4 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 9.0e-66
Identity = 128/178 (71.91%), Postives = 145/178 (81.46%), Query Frame = 0


Query: 1   MKTKIWVLEILLFLHYFFLGRAMA-------AEAGMPVMA-APVEGNTTFIDGTTWCVAK 60
           M  +IW L  L  L  F +G AMA       AEA MP+   +P EGNTTF+DGT+WCVAK
Sbjct: 1   MSIQIWGLVALFLLQSFLMGEAMATAVAVEKAEASMPMTTWSPPEGNTTFLDGTSWCVAK 60

Query: 61  PGVSQIDLQNALDWACGMGKADCRAIQKGGRCYEPDTLLSHASYAFNSYYQQNGNSDIAC 120
           PGVSQIDLQNALDWACG+GKADCRAIQKGGRCY+PDTL SHASYAFNSYYQQNGNSDIAC
Sbjct: 61  PGVSQIDLQNALDWACGLGKADCRAIQKGGRCYQPDTLFSHASYAFNSYYQQNGNSDIAC 120

Query: 121 NFGGCATLTKKDPSYGKCDYSASSSTNSAPSKSKNSPSHICLKLLSGVFVVLNLYFGS 171
           NFGG ATLTK+DPSYGKCDYSAS S +SAPSK K + S  CL+L+ G+++V+NLYFGS
Sbjct: 121 NFGGSATLTKRDPSYGKCDYSASRSASSAPSKWKKNGSRGCLQLVGGIWMVMNLYFGS 178

[Close]

BLAST of Cmc12g0328561 vs. ExPASy TrEMBL
Match: A0A6J1CF38 (major pollen allergen Ole e 10 OS=Momordica charantia OX=3673 GN=LOC111010934 PE=4 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 9.0e-66
Identity = 129/179 (72.07%), Postives = 146/179 (81.56%), Query Frame = 0


Query: 1   MKTKIW----VLEILLFLHYFFLGRAMA----AEAGMPVMA-APVEGNTTFIDGTTWCVA 60
           M+TKIW    VLE +  L   FLG  MA    AEA MP    +P EGN TF+DGT+WCVA
Sbjct: 1   MRTKIWVLLLVLEAMFLLQCCFLGEGMAVEEKAEAAMPATTLSPPEGNATFLDGTSWCVA 60

Query: 61  KPGVSQIDLQNALDWACGMGKADCRAIQKGGRCYEPDTLLSHASYAFNSYYQQNGNSDIA 120
           + G SQIDLQNALDWACG+GKADCRAIQKGG CY+PDTLLSHASYAFNS+YQQNGNSDIA
Sbjct: 61  RAGASQIDLQNALDWACGLGKADCRAIQKGGACYQPDTLLSHASYAFNSFYQQNGNSDIA 120

Query: 121 CNFGGCATLTKKDPSYGKCDYSASSSTNSAPSKSKNSPSHICLKLLSGVFVVLNLYFGS 171
           CNFGG ATLTKK+PSYGKCDYSASSS NSA SK KN+ SH+CL+L++G+ +VLNLY GS
Sbjct: 121 CNFGGSATLTKKNPSYGKCDYSASSSVNSATSKLKNNGSHVCLRLVAGILMVLNLYLGS 179

[Close]

BLAST of Cmc12g0328561 vs. ExPASy TrEMBL
Match: A0A6J1GUW0 (glucan endo-1,3-beta-glucosidase 13-like OS=Cucurbita moschata OX=3662 GN=LOC111457145 PE=4 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 3.8e-64
Identity = 127/175 (72.57%), Postives = 142/175 (81.14%), Query Frame = 0


Query: 1   MKTKIWVLEILLFLHYFFLGRAMA-------AEAGMPVMA-APVEGNTTFIDGTTWCVAK 60
           M T+IW L  L  L  F +G AMA       AEA MP+   +P EGNTTF+DGT+WCVAK
Sbjct: 1   MSTQIWGLVALFLLQSFLMGEAMATAVAVEKAEASMPMTTWSPPEGNTTFLDGTSWCVAK 60

Query: 61  PGVSQIDLQNALDWACGMGKADCRAIQKGGRCYEPDTLLSHASYAFNSYYQQNGNSDIAC 120
           PGVSQIDLQNALDWACG+GKADCRAIQKGGRCY+PDTL SHASYAFNSYYQQNGNSDIAC
Sbjct: 61  PGVSQIDLQNALDWACGLGKADCRAIQKGGRCYQPDTLFSHASYAFNSYYQQNGNSDIAC 120

Query: 121 NFGGCATLTKKDPSYGKCDYSASSSTNSAPSKSKNSPSHICLKLLSGVFVVLNLY 168
           NFGG ATLTK+DPSYGKCDYSAS ST+SA SK K +  H  LKL+ G+F+V+NLY
Sbjct: 121 NFGGSATLTKRDPSYGKCDYSASRSTSSARSKWKKNGRHGWLKLVGGIFMVMNLY 175

[Close]

BLAST of Cmc12g0328561 vs. TAIR 10
Match: AT4G05430.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 162.2 bits (409), Expect = 3.8e-40
Identity = 73/129 (56.59%), Postives = 94/129 (72.87%), Query Frame = 0


Query: 37  EGNTTFIDGTTWCVAKPGVSQIDLQNALDWACGMGKADCRAIQKGGRCYEPDTLLSHASY 96
           +GNTTF++GTTWCVA+PG +Q +LQ ALDWACG+G+ DC  I++ G CYEPDT++SHAS+
Sbjct: 12  QGNTTFLEGTTWCVARPGATQAELQRALDWACGIGRVDCSVIERHGDCYEPDTIVSHASF 71

Query: 97  AFNSYYQQNGNSDIACNFGGCATLTKKDPSYGKCDYSASSSTNSAPSKSKNSPSHICLKL 156
           AFN+YYQ NGN+ IAC FGG AT TK +PSYGKC Y AS S  S         +   L +
Sbjct: 72  AFNAYYQTNGNNRIACYFGGTATFTKINPSYGKCSYDASKSEVSTARSLSEYKARWLLLM 131

Query: 157 LSGVFVVLN 166
             GVF +++
Sbjct: 132 YIGVFSLIS 140

[Close]

BLAST of Cmc12g0328561 vs. TAIR 10
Match: AT1G09460.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 128.3 bits (321), Expect = 6.1e-30
Identity = 62/122 (50.82%), Postives = 84/122 (68.85%), Query Frame = 0


Query: 29  MPVMAAPVEGNTTFIDGTTWCVAKPGVSQIDLQNALDWACGMGKADCRAIQKGGRCYEPD 88
           +PV+A PV  N+  + G +WCVAKPG SQ+ LQ ALD+ACG+  ADC  +Q+GG CY P 
Sbjct: 118 VPVVAPPVVSNSPSVSGQSWCVAKPGASQVSLQQALDYACGI--ADCSQLQQGGNCYSPI 177

Query: 89  TLLSHASYAFNSYYQQNGNSDIACNFGGCATLTKKDPSYGKCDYSASSSTNSAPSKSKNS 148
           +L SHAS+AFNSYYQ+N  S  +C+FGG A+L   +PS G C Y   SST++  +    +
Sbjct: 178 SLQSHASFAFNSYYQKN-PSPQSCDFGGAASLVNTNPSTGSCIYQTGSSTSTPMTAGTTT 236

Query: 149 PS 151
           P+
Sbjct: 238 PT 236

[Close]

BLAST of Cmc12g0328561 vs. TAIR 10
Match: AT1G29380.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 118.6 bits (296), Expect = 4.8e-27
Identity = 58/102 (56.86%), Postives = 70/102 (68.63%), Query Frame = 0


Query: 48  WCVAKPGVSQIDLQNALDWACGMGKADCRAIQKGGRCYEPDTLLSHASYAFNSYYQQNGN 107
           WC+AK   S   LQ ALD+ACG G ADC  IQ+G  CYEP+T+  HAS+AFNSYYQ++  
Sbjct: 148 WCIAKANASPTSLQVALDYACGYGGADCGQIQQGAACYEPNTIRDHASFAFNSYYQKHPG 207

Query: 108 SDIACNFGGCATLTKKDPSYGKCDYSASSSTNSAPSKSKNSP 150
           SD +CNFGG A LT  DPS G C +S+SS T S    S+ SP
Sbjct: 208 SD-SCNFGGAAQLTSTDPSKGSCHFSSSSGTVSTSPPSQMSP 248

[Close]

BLAST of Cmc12g0328561 vs. TAIR 10
Match: AT4G29360.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 117.1 bits (292), Expect = 1.4e-26
Identity = 56/117 (47.86%), Postives = 71/117 (60.68%), Query Frame = 0


Query: 30  PVMAAPVEGNTTFIDG-------TTWCVAKPGVSQIDLQNALDWACGMGKADCRAIQKGG 89
           P  +  + GN+T   G         WC+A    S  +LQ ALDWACG G  DC A+Q   
Sbjct: 366 PSSSPIINGNSTVTIGGGGGGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQ 425

Query: 90  RCYEPDTLLSHASYAFNSYYQQNGNSDIACNFGGCATLTKKDPSYGKCDYSASSSTN 140
            C+EPDT+LSHASYAFN+YYQQ+G S I C+F G +    KDPSYG C Y  + +T+
Sbjct: 426 PCFEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDKDPSYGNCLYMIAPATD 482

[Close]

BLAST of Cmc12g0328561 vs. TAIR 10
Match: AT5G56590.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 115.9 bits (289), Expect = 3.1e-26
Identity = 60/142 (42.25%), Postives = 83/142 (58.45%), Query Frame = 0


Query: 38  GNTTFIDGTTWCVAKPGVSQIDLQNALDWACGMGKADCRAIQKGGRCYEPDTLLSHASYA 97
           G  +     +WC+A    S+ DL+ ALDWACG G  DC AIQ    C++PDTL+SHAS+ 
Sbjct: 359 GTNSSGSSNSWCIASSKASERDLKGALDWACGPGNVDCTAIQPSQPCFQPDTLVSHASFV 418

Query: 98  FNSYYQQNGNSDIACNFGGCATLTKKDPSYGKCDY-----------SASSSTNSAPSKSK 157
           FNSY+QQN  +D+AC+FGG      KDPSY KC Y           +A++ T+SA +   
Sbjct: 419 FNSYFQQNRATDVACSFGGAGVKVNKDPSYDKCIYITAGGNKTKATNATALTSSASTPRG 478

Query: 158 NSPSHICLKLLSGVFVVLNLYF 169
           N      LKL     ++++L+F
Sbjct: 479 NELLQWILKLC----LMISLFF 496

The following BLAST results are available for this feature:

BLAST of Cmc12g0328561 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008444815.1	2.3e-92	99.41	PREDICTED: glucan endo-1,3-beta-glucosidase 13 [Cucumis melo]	[more]
XP_004135538.1	1.9e-86	94.74	major pollen allergen Ole e 10 [Cucumis sativus] >KAE8653300.1 hypothetical prot...	[more]
XP_038895270.1	2.1e-77	84.39	major pollen allergen Ole e 10 [Benincasa hispida]	[more]
XP_022140209.1	1.9e-65	72.07	major pollen allergen Ole e 10 [Momordica charantia]	[more]
XP_022994908.1	1.9e-65	71.91	major pollen allergen Ole e 10-like [Cucurbita maxima]	[more]

BLAST of Cmc12g0328561 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VYE5	2.0e-25	47.86	Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360...	[more]
Q9FJU9	4.4e-25	42.25	Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590...	[more]
Q9C7U5	7.0e-23	47.37	Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 ...	[more]
P52409	9.1e-23	52.27	Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1	[more]
Q9ZU91	1.2e-22	46.09	Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 ...	[more]

BLAST of Cmc12g0328561 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BB84	1.1e-92	99.41	glucan endo-1,3-beta-glucosidase 13 OS=Cucumis melo OX=3656 GN=LOC103488047 PE=4...	[more]
A0A0A0M121	3.5e-78	88.89	X8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G537410 PE=4 SV=...	[more]
A0A6J1K6E1	9.0e-66	71.91	major pollen allergen Ole e 10-like OS=Cucurbita maxima OX=3661 GN=LOC111490492 ...	[more]
A0A6J1CF38	9.0e-66	72.07	major pollen allergen Ole e 10 OS=Momordica charantia OX=3673 GN=LOC111010934 PE...	[more]
A0A6J1GUW0	3.8e-64	72.57	glucan endo-1,3-beta-glucosidase 13-like OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]

BLAST of Cmc12g0328561 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G05430.1	3.8e-40	56.59	Carbohydrate-binding X8 domain superfamily protein	[more]
AT1G09460.1	6.1e-30	50.82	Carbohydrate-binding X8 domain superfamily protein	[more]
AT1G29380.1	4.8e-27	56.86	Carbohydrate-binding X8 domain superfamily protein	[more]
AT4G29360.1	1.4e-26	47.86	O-Glycosyl hydrolases family 17 protein	[more]
AT5G56590.1	3.1e-26	42.25	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 47..132 e-value: 1.2E-49 score: 181.0
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 47..119 e-value: 3.4E-23 score: 82.1
None	No IPR available	GENE3D	1.20.58.1040		coord: 41..147 e-value: 1.4E-12 score: 49.8
None	No IPR available	PANTHER	PTHR31044:SF36	CARBOHYDRATE-BINDING X8 DOMAIN SUPERFAMILY PROTEIN	coord: 31..133
IPR044788	Carbohydrate-binding X8 domain-containing protein, plant	PANTHER	PTHR31044	BETA-1,3 GLUCANASE	coord: 31..133

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cmc12g0328561.1	Cmc12g0328561.1	mRNA