Cmc12g0328501 (gene) Melon (Charmono) v1.1

Overview
NameCmc12g0328501
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionPolyprenol reductase
LocationCMiso1.1chr12: 19663311 .. 19664496 (-)
RNA-Seq ExpressionCmc12g0328501
SyntenyCmc12g0328501
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTTCCTGCCAACCAGTTGACCGGAGGTTCAAGTTTGTTTGCATGGCAAAAATCTCATTCATCTTGGAGGGAACATGGATTCTTGGTCTGGAAAGCGGTTTTTCTGATTTTATTAATGGAAGTTCAAGTTTTGAGACGTCTTTATGAAACAATATACGTTTTCAACTATAGTCCCTCGGCTCGGATGCACATCTTTGGATATTTAACTGGCTTGTTGTAAGTACCACTTTGAAGCTTTCTTTAGTTGGTTTGTTGCAATATATATTCACTGGTTTTAATATAGCTAGTTTCTTTGTACTTTCGACCTTGTTTTACTACGTTGTTGAAAGACTTAAGTGCTAGAATCAAGACTATTTGCTTCTCAGGATGGAAAATACTTTCTGGGTCAATTTGAATTTGCATTTTTTAGATTAATGTATATTATTTCGTTTTTCATATGTAAGGATGTAGTATGATTCATTCTTTTAGGCTCTAGTTTTTTCATGGATGATTGTGTTAGATACCTAGATTAGTATAAGTCAGGGGTATAAAGGTAATTGGTTATGTTGGCAGTTAGATGGTTATAAATAGGATGTTGGGAAGAGAAGAGAGGGGTATGAAGATTTTGGTGAAGGAAAGGGCTCTCTTATTCCTTAAGAGATAGGAGAAGCAGAAGGATCAGGTTTCTTTGTTCTTGCTATTTTGTTATTACGTTTTTTTTTCTTCTTTTCTGTGTTTTGGATTAGAAGTATTTCATTGTTAGGGAAGGACTGTTATTGTAGTTCATTGTTGGGAACTACTTTTGCTTTCATATATTGTAATTTTCCTTTGAGATATCAATAAAGAATAGACACTACCTTAGTGTTCTAATAGATTGCTATTTTGGTGGTTCATTGTTCTTTGTTGTAGTTCAATCCTGACGTCTAGAGGCTTGTTATGTTGATCTCTATTATTTTTGATTAGTTTCTATACAGCAGCTCCTCTATCACTTTGTAGCAATTGTATTCCAGAAGTGTATCACTTTGCCACTTTGCCGCAAGTGGGCTAGCTCAGTTTATTGTTCAAGGAAGAAAGCATATGCCTGATGTTGAAATTAGCCCGTTGGATGCTGTGATTCCTTTATCAAATTTAGGTTGGTGTCAGTGGATTGGTGCAGCTTTATTCTTTTGGGGTTGGATTCATCAGCATTGTTGCCATCAGATTCTG

mRNA sequence

TTTTTCCTGCCAACCAGTTGACCGGAGGTTCAAGTTTGTTTGCATGGCAAAAATCTCATTCATCTTGGAGGGAACATGGATTCTTGGTCTGGAAAGCGGTTTTTCTGATTTTATTAATGGAAGTTCAAGTTTTGAGACGTCTTTATGAAACAATATACGTTTTCAACTATAGTCCCTCGGCTCGGATGCACATCTTTGGATATTTAACTGGCTTGTTTTTCTATACAGCAGCTCCTCTATCACTTTGTAGCAATTGTATTCCAGAAGTGTATCACTTTGCCACTTTGCCGCAAGTGGGCTAGCTCAGTTTATTGTTCAAGGAAGAAAGCATATGCCTGATGTTGAAATTAGCCCGTTGGATGCTGTGATTCCTTTATCAAATTTAGGTTGGTGTCAGTGGATTGGTGCAGCTTTATTCTTTTGGGGTTGGATTCATCAGCATTGTTGCCATCAGATTCTG

Coding sequence (CDS)

ATGGAAGTTCAAGTTTTGAGACGTCTTTATGAAACAATATACGTTTTCAACTATAGTCCCTCGGCTCGGATGCACATCTTTGGATATTTAACTGGCTTGTTTTTCTATACAGCAGCTCCTCTATCACTTTGTAGCAATTGTATTCCAGAAGTGTATCACTTTGCCACTTTGCCGCAAGTGGGCTAG

Protein sequence

MEVQVLRRLYETIYVFNYSPSARMHIFGYLTGLFFYTAAPLSLCSNCIPEVYHFATLPQVG
Homology
BLAST of Cmc12g0328501 vs. NCBI nr
Match: XP_008444886.1 (PREDICTED: polyprenol reductase 2 [Cucumis melo])

HSP 1 Score: 116.3 bits (290), Expect = 9.2e-23
Identity = 55/55 (100.00%), Postives = 55/55 (100.00%), Query Frame = 0

Query: 1   MEVQVLRRLYETIYVFNYSPSARMHIFGYLTGLFFYTAAPLSLCSNCIPEVYHFA 56
           MEVQVLRRLYETIYVFNYSPSARMHIFGYLTGLFFYTAAPLSLCSNCIPEVYHFA
Sbjct: 136 MEVQVLRRLYETIYVFNYSPSARMHIFGYLTGLFFYTAAPLSLCSNCIPEVYHFA 190

BLAST of Cmc12g0328501 vs. NCBI nr
Match: XP_004148545.1 (polyprenol reductase 2 [Cucumis sativus] >KGN43186.1 hypothetical protein Csa_020422 [Cucumis sativus])

HSP 1 Score: 114.4 bits (285), Expect = 3.5e-22
Identity = 54/55 (98.18%), Postives = 55/55 (100.00%), Query Frame = 0

Query: 1   MEVQVLRRLYETIYVFNYSPSARMHIFGYLTGLFFYTAAPLSLCSNCIPEVYHFA 56
           MEVQVLRRLYETIYVFNYSPSARMHIFGYLTGLFFYTAAPLSLCS+CIPEVYHFA
Sbjct: 136 MEVQVLRRLYETIYVFNYSPSARMHIFGYLTGLFFYTAAPLSLCSSCIPEVYHFA 190

BLAST of Cmc12g0328501 vs. NCBI nr
Match: KAG6588412.1 (Polyprenol reductase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 113.2 bits (282), Expect = 7.8e-22
Identity = 53/55 (96.36%), Postives = 53/55 (96.36%), Query Frame = 0

Query: 1   MEVQVLRRLYETIYVFNYSPSARMHIFGYLTGLFFYTAAPLSLCSNCIPEVYHFA 56
           MEVQVLRRLYETIY FNYSPSARMH FGYLTGLFFYTAAPLSLCSNCIPEVYHFA
Sbjct: 136 MEVQVLRRLYETIYTFNYSPSARMHFFGYLTGLFFYTAAPLSLCSNCIPEVYHFA 190

BLAST of Cmc12g0328501 vs. NCBI nr
Match: KAG7022255.1 (Polyprenol reductase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 113.2 bits (282), Expect = 7.8e-22
Identity = 53/55 (96.36%), Postives = 53/55 (96.36%), Query Frame = 0

Query: 1   MEVQVLRRLYETIYVFNYSPSARMHIFGYLTGLFFYTAAPLSLCSNCIPEVYHFA 56
           MEVQVLRRLYETIY FNYSPSARMH FGYLTGLFFYTAAPLSLCSNCIPEVYHFA
Sbjct: 136 MEVQVLRRLYETIYTFNYSPSARMHFFGYLTGLFFYTAAPLSLCSNCIPEVYHFA 190

BLAST of Cmc12g0328501 vs. NCBI nr
Match: XP_022933967.1 (polyprenol reductase 2-like [Cucurbita moschata] >XP_022933968.1 polyprenol reductase 2-like [Cucurbita moschata])

HSP 1 Score: 112.1 bits (279), Expect = 1.7e-21
Identity = 53/56 (94.64%), Postives = 53/56 (94.64%), Query Frame = 0

Query: 1   MEVQVLRRLYETIYVFNYSPSARMHIFGYLTGLFFYTAAPLSLCSNCIPEVYHFAT 57
           MEVQVLRRLYETIY F YSPSARMH FGYLTGLFFYTAAPLSLCSNCIPEVYHFAT
Sbjct: 136 MEVQVLRRLYETIYTFIYSPSARMHFFGYLTGLFFYTAAPLSLCSNCIPEVYHFAT 191

BLAST of Cmc12g0328501 vs. ExPASy Swiss-Prot
Match: A2XWN6 (Polyprenol reductase 1 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_17065 PE=3 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 6.0e-17
Identity = 41/54 (75.93%), Postives = 47/54 (87.04%), Query Frame = 0

Query: 1   MEVQVLRRLYETIYVFNYSPSARMHIFGYLTGLFFYTAAPLSLCSNCIPEVYHF 55
           ME+QVLRRLYET +VF+YSPSARMHI GYLTGLF+Y AAPLSL S+CIPE   +
Sbjct: 153 MEIQVLRRLYETEHVFHYSPSARMHIVGYLTGLFYYVAAPLSLASSCIPEAAEY 206

BLAST of Cmc12g0328501 vs. ExPASy Swiss-Prot
Match: Q7XUH5 (Polyprenol reductase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0576800 PE=2 SV=2)

HSP 1 Score: 87.4 bits (215), Expect = 6.0e-17
Identity = 41/54 (75.93%), Postives = 47/54 (87.04%), Query Frame = 0

Query: 1   MEVQVLRRLYETIYVFNYSPSARMHIFGYLTGLFFYTAAPLSLCSNCIPEVYHF 55
           ME+QVLRRLYET +VF+YSPSARMHI GYLTGLF+Y AAPLSL S+CIPE   +
Sbjct: 153 MEIQVLRRLYETEHVFHYSPSARMHIVGYLTGLFYYVAAPLSLASSCIPEAAEY 206

BLAST of Cmc12g0328501 vs. ExPASy Swiss-Prot
Match: Q9SI62 (Polyprenol reductase 2 OS=Arabidopsis thaliana OX=3702 GN=PPRD2 PE=1 SV=2)

HSP 1 Score: 81.6 bits (200), Expect = 3.3e-15
Identity = 39/54 (72.22%), Postives = 41/54 (75.93%), Query Frame = 0

Query: 1   MEVQVLRRLYETIYVFNYSPSARMHIFGYLTGLFFYTAAPLSLCSNCIPEVYHF 55
           ME+ VLRRL E+ YVF YSPSARMHI GY  GLFFY  APLSLCSN  PEV  F
Sbjct: 141 MEIHVLRRLIESFYVFKYSPSARMHILGYFAGLFFYVTAPLSLCSNIAPEVAGF 194

BLAST of Cmc12g0328501 vs. ExPASy Swiss-Prot
Match: B8B6G5 (Polyprenol reductase 2 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_26070 PE=3 SV=2)

HSP 1 Score: 81.6 bits (200), Expect = 3.3e-15
Identity = 41/54 (75.93%), Postives = 46/54 (85.19%), Query Frame = 0

Query: 1   MEVQVLRRLYETIYVFNYSPSARMHIFGYLTGLFFYTAAPLSLCSNCIPEVYHF 55
           MEVQVLRRLYET +VF+YSP ARMHI GYLTGLF+Y AAPLSL S+CIPE   +
Sbjct: 153 MEVQVLRRLYETEHVFHYSP-ARMHIVGYLTGLFYYVAAPLSLASSCIPEAAEY 205

BLAST of Cmc12g0328501 vs. ExPASy Swiss-Prot
Match: Q7F0Q2 (Polyprenol reductase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os07g0493400 PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 3.3e-15
Identity = 41/54 (75.93%), Postives = 46/54 (85.19%), Query Frame = 0

Query: 1   MEVQVLRRLYETIYVFNYSPSARMHIFGYLTGLFFYTAAPLSLCSNCIPEVYHF 55
           MEVQVLRRLYET +VF+YSP ARMHI GYLTGLF+Y AAPLSL S+CIPE   +
Sbjct: 153 MEVQVLRRLYETEHVFHYSP-ARMHIVGYLTGLFYYVAAPLSLASSCIPEAAEY 205

BLAST of Cmc12g0328501 vs. ExPASy TrEMBL
Match: A0A1S3BBF0 (Polyprenol reductase OS=Cucumis melo OX=3656 GN=LOC103488097 PE=3 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 4.4e-23
Identity = 55/55 (100.00%), Postives = 55/55 (100.00%), Query Frame = 0

Query: 1   MEVQVLRRLYETIYVFNYSPSARMHIFGYLTGLFFYTAAPLSLCSNCIPEVYHFA 56
           MEVQVLRRLYETIYVFNYSPSARMHIFGYLTGLFFYTAAPLSLCSNCIPEVYHFA
Sbjct: 136 MEVQVLRRLYETIYVFNYSPSARMHIFGYLTGLFFYTAAPLSLCSNCIPEVYHFA 190

BLAST of Cmc12g0328501 vs. ExPASy TrEMBL
Match: A0A0A0K5R7 (Polyprenol reductase OS=Cucumis sativus OX=3659 GN=Csa_7G007840 PE=3 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.7e-22
Identity = 54/55 (98.18%), Postives = 55/55 (100.00%), Query Frame = 0

Query: 1   MEVQVLRRLYETIYVFNYSPSARMHIFGYLTGLFFYTAAPLSLCSNCIPEVYHFA 56
           MEVQVLRRLYETIYVFNYSPSARMHIFGYLTGLFFYTAAPLSLCS+CIPEVYHFA
Sbjct: 136 MEVQVLRRLYETIYVFNYSPSARMHIFGYLTGLFFYTAAPLSLCSSCIPEVYHFA 190

BLAST of Cmc12g0328501 vs. ExPASy TrEMBL
Match: A0A6J1F6C0 (Polyprenol reductase OS=Cucurbita moschata OX=3662 GN=LOC111441221 PE=3 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 8.4e-22
Identity = 53/56 (94.64%), Postives = 53/56 (94.64%), Query Frame = 0

Query: 1   MEVQVLRRLYETIYVFNYSPSARMHIFGYLTGLFFYTAAPLSLCSNCIPEVYHFAT 57
           MEVQVLRRLYETIY F YSPSARMH FGYLTGLFFYTAAPLSLCSNCIPEVYHFAT
Sbjct: 136 MEVQVLRRLYETIYTFIYSPSARMHFFGYLTGLFFYTAAPLSLCSNCIPEVYHFAT 191

BLAST of Cmc12g0328501 vs. ExPASy TrEMBL
Match: A0A6J1I5R2 (Polyprenol reductase OS=Cucurbita maxima OX=3661 GN=LOC111469837 PE=3 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 3.2e-21
Identity = 52/55 (94.55%), Postives = 52/55 (94.55%), Query Frame = 0

Query: 1   MEVQVLRRLYETIYVFNYSPSARMHIFGYLTGLFFYTAAPLSLCSNCIPEVYHFA 56
           MEVQVLRRLYETIY FNYSPSARMH FGYLTGLFFYTAAPLSLCSNCI EVYHFA
Sbjct: 136 MEVQVLRRLYETIYTFNYSPSARMHFFGYLTGLFFYTAAPLSLCSNCITEVYHFA 190

BLAST of Cmc12g0328501 vs. ExPASy TrEMBL
Match: A0A6J1C196 (Polyprenol reductase OS=Momordica charantia OX=3673 GN=LOC111007527 PE=3 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 4.6e-20
Identity = 52/56 (92.86%), Postives = 52/56 (92.86%), Query Frame = 0

Query: 1   MEVQVLRRLYETIYVFNYSPSARMHIFGYLTGLFFYTAAPLSLCSNCIPEVYHFAT 57
           MEVQVLRRLYETIYVFNYS SARMHI GYLTG FFYTAAPLSLCSN IPEVYHFAT
Sbjct: 136 MEVQVLRRLYETIYVFNYSRSARMHICGYLTGFFFYTAAPLSLCSNFIPEVYHFAT 191

BLAST of Cmc12g0328501 vs. TAIR 10
Match: AT2G16530.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 81.6 bits (200), Expect = 2.3e-16
Identity = 39/54 (72.22%), Postives = 41/54 (75.93%), Query Frame = 0

Query: 1   MEVQVLRRLYETIYVFNYSPSARMHIFGYLTGLFFYTAAPLSLCSNCIPEVYHF 55
           ME+ VLRRL E+ YVF YSPSARMHI GY  GLFFY  APLSLCSN  PEV  F
Sbjct: 141 MEIHVLRRLIESFYVFKYSPSARMHILGYFAGLFFYVTAPLSLCSNIAPEVAGF 194

BLAST of Cmc12g0328501 vs. TAIR 10
Match: AT2G16530.2 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 81.6 bits (200), Expect = 2.3e-16
Identity = 39/54 (72.22%), Postives = 41/54 (75.93%), Query Frame = 0

Query: 1   MEVQVLRRLYETIYVFNYSPSARMHIFGYLTGLFFYTAAPLSLCSNCIPEVYHF 55
           ME+ VLRRL E+ YVF YSPSARMHI GY  GLFFY  APLSLCSN  PEV  F
Sbjct: 140 MEIHVLRRLIESFYVFKYSPSARMHILGYFAGLFFYVTAPLSLCSNIAPEVAGF 193

BLAST of Cmc12g0328501 vs. TAIR 10
Match: AT1G72590.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 73.6 bits (179), Expect = 6.3e-14
Identity = 35/54 (64.81%), Postives = 40/54 (74.07%), Query Frame = 0

Query: 1   MEVQVLRRLYETIYVFNYSPSARMHIFGYLTGLFFYTAAPLSLCSNCIPEVYHF 55
           ME+ VLRR+ E+ YVF YS SARMHI  Y+  LF+Y AAPLSLCSN  PEV  F
Sbjct: 120 MEIHVLRRVIESFYVFKYSTSARMHILAYVGALFYYVAAPLSLCSNIAPEVARF 173

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008444886.19.2e-23100.00PREDICTED: polyprenol reductase 2 [Cucumis melo][more]
XP_004148545.13.5e-2298.18polyprenol reductase 2 [Cucumis sativus] >KGN43186.1 hypothetical protein Csa_02... [more]
KAG6588412.17.8e-2296.36Polyprenol reductase 2, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7022255.17.8e-2296.36Polyprenol reductase 2 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022933967.11.7e-2194.64polyprenol reductase 2-like [Cucurbita moschata] >XP_022933968.1 polyprenol redu... [more]
Match NameE-valueIdentityDescription
A2XWN66.0e-1775.93Polyprenol reductase 1 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_17065 PE=3 ... [more]
Q7XUH56.0e-1775.93Polyprenol reductase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0576800 ... [more]
Q9SI623.3e-1572.22Polyprenol reductase 2 OS=Arabidopsis thaliana OX=3702 GN=PPRD2 PE=1 SV=2[more]
B8B6G53.3e-1575.93Polyprenol reductase 2 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_26070 PE=3 ... [more]
Q7F0Q23.3e-1575.93Polyprenol reductase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os07g0493400 ... [more]
Match NameE-valueIdentityDescription
A0A1S3BBF04.4e-23100.00Polyprenol reductase OS=Cucumis melo OX=3656 GN=LOC103488097 PE=3 SV=1[more]
A0A0A0K5R71.7e-2298.18Polyprenol reductase OS=Cucumis sativus OX=3659 GN=Csa_7G007840 PE=3 SV=1[more]
A0A6J1F6C08.4e-2294.64Polyprenol reductase OS=Cucurbita moschata OX=3662 GN=LOC111441221 PE=3 SV=1[more]
A0A6J1I5R23.2e-2194.55Polyprenol reductase OS=Cucurbita maxima OX=3661 GN=LOC111469837 PE=3 SV=1[more]
A0A6J1C1964.6e-2092.86Polyprenol reductase OS=Momordica charantia OX=3673 GN=LOC111007527 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G16530.12.3e-1672.223-oxo-5-alpha-steroid 4-dehydrogenase family protein [more]
AT2G16530.22.3e-1672.223-oxo-5-alpha-steroid 4-dehydrogenase family protein [more]
AT1G72590.16.3e-1464.813-oxo-5-alpha-steroid 4-dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR14624:SF2POLYPRENOL REDUCTASE 2-LIKEcoord: 1..56
IPR039698Polyprenol reductasePANTHERPTHR14624DFG10 PROTEINcoord: 1..56

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc12g0328501.1Cmc12g0328501.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006488 dolichol-linked oligosaccharide biosynthetic process
molecular_function GO:0003865 3-oxo-5-alpha-steroid 4-dehydrogenase activity