Cmc12g0327701 (gene) Melon (Charmono) v1.1

Overview
NameCmc12g0327701
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionFAD-binding Berberine family protein
LocationCMiso1.1chr12: 18730665 .. 18734975 (+)
RNA-Seq ExpressionCmc12g0327701
SyntenyCmc12g0327701
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTGGTTATCATTATAAATAACTCCCTTTTCTACTGCAATATATATATCTTTCTAAAACCAAGAAGAAATAAAAAGCAAACACTAAAATGCAAATTTTAAGGTCTTCAATTTTGTTCTTATCCTTTTCATTCCTTGTGTTTTTTTCTTCTGCATCTTCTTCAGCTCCTGAAAATTTCTTGCAATGTCTTCTCCGACACTCTCCACCATCGTACCCCATCGCTCCGGCGATCTTTACTCCAAACAATGCTTCTTATTCCTCTGTTCTTGAGACTTACATTAGAAACTTGAGATTCAACACTTCAACAACTCCTAAACCATTCCTCATTCTCACTGCTCTCCATGAATCTCATGTTAAAGTTGCTATTCTTTGTGCTCGAATCCATAATCTTCAGATGAAGATTCGTAGCGGCGGCCATGATTACGAAGGCGTTTCTTACGTCTCCGACGTCGTCCCTTTCTTCGTACTCGATATGTTCAACCTACGAGAGATAACGGTTGATGCCAAAACCGAGACCGCTTGGGTATTGTCAATCATTCATTATTTATATTGATATACGTATTGTCTCACTCCACTTATAAACTTGAAAATTACGAGAAACACTTACGTGTTGTCTCTTTGCGGCCTATCTTTTGTAAATCAAGGCAGCTTTCATTTCTTATTTTTTAAATGATTTTTTATAACTTATTATCAATATTTATTACTGAGAAAATAACATAATGCAGTGGTAAATGACCTATGCTATAACGAACTTAATTAAAATTCATATTTTTACCAGGTCCAAACCGGAGCAACTCTAGGAGAAGTGTATTATAGAGTTGCTGAGAAAAATAAGTCCTACGGCTTTCCCGCCGGCGTGTGCCCAACGGTGGGGGTCGGCGGTCATTTTGGTGGTGGCGGCTATGGTAACATGATGAGAAAACACGGCCTCTCGGTAGATCAAATAATCGATGCAAAGATTATCGACGTCAATGGAAAACTCCTTGACCGAAAGGCAATGGGGGAGGATCTATTTTGGGCCATTATAGGCGGTGGAGGATCTAGCTTCGGTGTGGTGGTAGCATACAAAATCCGAATGGTTCGTGTGCCGGAGAGGGTAACGGTGTTTCGAGTACAAAGAACATTGGAACAAAATGAGTTAACGGAGATTGTAGATGAGTGGCAACAGGTGGCACATGTGATTGACAATGATTTGTTCATAAGAGTAACGTTTGACGTTGTGAATGGAACAGACAAGGAAAAAAAGACATTGAGAGCTACGTTTATAGCCTTATTCCTAGGAGATTCCGGAAGGCTTTTGTTTGTCATCAACAACAGTTTTCCTAAACTTGGTTTGAAGAAATCTGATTGCATTGAAATGAGTTGGCTTCAATCTGTGCTCTTTTGGACTAATTTCCCTCCAGGTAATCTTTCTTTTTATTTTGGATATATATAAAGAGTTTGTTCTTGGTAAAATTAATCACTTCTTTTGGGTTGTTTTTCAAATGTTTTACAAAACCTCCATAAAACAGAATATTACACATGATGGGAATTAAGAAAACTTGTGCCACTTTTTTTTTTCCTTTTTTTCTTTTTTAATAGAAATGTAGAATATTTCTATTAAAAATTACAGAAAACTTGTGCCACAATATTTAGTAATTGTTGTTTTCTTCTTTCTTGTTTTATTAGTATTTTTACTTATTTTTATGAAAAGAAATAAGACTTTTGGTGGTTCTTTTCTTTTTTTTAATGGAAAAATGATTCGTACATGTATATAGAAAGTTTTCAAATATGAAGTATAATAATGCCCTTTTAATAAGATTTTTACTAATTTTTTAAAATACTCAAACCAGATTTTGTAAACTTTAAATAAATGGGTAATTAGAAGATGGAGCAATTTTATAAATAATAATTAAGTATATAGTAATATTAAAAAAATGTAAATATAATAAAGTTTATCAATAACAGACCGATATTTGCAACATAGTCTATCTATGTGATAGACTCTTATCATTTATAGACTTTGATAGATTTTGCTATATATATTTGCATTTTTTAGATGGTATTATAAACTTAATTATTTTGAATCTAATTGTTATATTTGTAAATATCCCAAAGAATAGTTTGAATCATCTTTTGTTCACTAAAAAATATAAAAAGTTGGAAGGAAATAAGTTAATTTTCCAAAACCATAAACGAAAAATGAACCTATGTCTAATCATTTGAATTTTAGTTATTTTTTTCTTTTAGAAAAATGAACTTACAATCAATACTCTTGCTTTTATGTTTTTAAAAAATATATTATTTTTCTACCAATGTTTTAAAAACGTAAAGTCAATCTTTTTAATTAAAATAAAAACTAGATAAATACTTTTAAATGATAAAATTTCTAAAAATAATCTTAAAAATGCTTACAAATAGCAAAATATCACAATCAATGTGCGACAGGTCAAGATAGACTACTAGACACATGATATAAATAGACAAAAATAATAGTTTATCTTGGTCTATTGTGTCCATCATAAATAGACTTTTTAAAAATATTTTAATTAATTTTGCTATATTTGAAAATAACACTAATTGTTTTAAAATCACCGAATCCAAATGTTTAATTTAAGAAAAGTTAAAAAAAAAAAAAGAAAGAAAAAAGAAAATTAAGTATAGCTTTCAAAATCAAAATAGTTATAACTTAATTAAGAGATTACATAATAGATTTGTGATTTAAAACCGAACTCCTTTTAAACAAAAGAAAAACTTGGGTGCTCTTTTTGGCCTTCAATCCATGGGATTGCCAAATAAAATTTGTATGTGTGACAAATACTTTTAAGAAAAAAAAAATTCAATTTGTTTTTGTTTTTTGTTTTATAAATTGATGCACACCCTCCCTACCTCTAAAATTGAATTTGAGAAAAAATGTCATTATCCTTAAAAAATTTGGAATCAATTTTTTTGAAAGAAAAATATCTTTTTTATTGTTGAATTCAAAATTCCTCACAAATCTGTCCTACCATGACTAGACAACACATCATAATATTTTAAATACTAATTTGAACCTATTTATTTTTTTAAATTAATTTATTTGACTTTACTATAAGTATTTAGATCCCACCAAAATACAAAACTATGAAAAGAAAAAAAAAAAAAACAAAAAACCAAAAACACTTCTTTTAACGACCCAACCCAATTATAATTGTTTTGAAAAATTATTTTAAATGATAAGACTCTGAAATATACTTATAAATGTAGCAAAAATATCACAATCTATTTACGTATGATGGTATCTATGTCTATAATGACGTATATCAATACAAATAAGTAGTACATTATTGTCTATATATCACATATAAACATCAAATTTTGTTATATTTGTAAATAGTTTGCGATATATATGTTAATTAGGAGTAGAATTTGTTTGATACTCAGAAATGGTAGAAAATAATATAAAGAAACTGTAGAAAAAAATAAATCTTTGAAAGGACAGACCCTATCTAAAAGTTATCATTTTATGAAATACTTGTGACCACAGGCACATCAGTGGAAGCCCTACTATCCAGAACCCCACAAGTACTGACCCATCTAAAAAGAAAGTCAGATTATGTAAAAGCCCCAATTCCAAAGGAAGGACTCAACAAAATATGGAAGAAAATGATAGAACTTGAAAAACCCATGATGACATTTAACCCTTATGGTGGAAGAATGGCAGAAATTCCATCGAATGCAACTCCATTTCCTCATAGAGCTGGAAACTTATGGAAGATTCAATATGCAACAAATTGGGATGAAGAAGGAAAAAGAGAAGCCAAACATTTTATTGATTTGACAAGGAAACTCTACAAGTTTATGACACCATTTGTGTCAAAAAATCCCAGAACTGCCTTTTTGAACTATAGAGATCTTGATATTGGTGTAAATCACAATGGGAAGAATAGTTACTATGAAGGAAGAGTTTATGGCATTAAATACTTTGAAGGTAACTTTGAGAGGTTGGTGAAGATCAAGACCAAGGTTGATCCTCACAACTTTTTTAGGAATGAACAAAGCATTCCAAGGTTTCCTCATGTTTGAAGATAGAAAATGATTGATTGTGTGTTATTGTTTCTCTTTTGTTGTTTTCTTTGTTCATTGTCATTTCAAGATTAACTTTTTCTTTTCTAAATAATATAAAGTGTGTAAGTTATATATAGTCGTTATATATTATATTATATTATATAGTTGTATATGTTGGATAGATGTGATGATTAGATTATAAAGCCATTACTGATAATGTATCTTTATGTACGTGAAAATGAAAGGGCTCCTTCTAAGTTTATCACATATTCAAATTAATTAGATGT

mRNA sequence

CTGGTTATCATTATAAATAACTCCCTTTTCTACTGCAATATATATATCTTTCTAAAACCAAGAAGAAATAAAAAGCAAACACTAAAATGCAAATTTTAAGGTCTTCAATTTTGTTCTTATCCTTTTCATTCCTTGTGTTTTTTTCTTCTGCATCTTCTTCAGCTCCTGAAAATTTCTTGCAATGTCTTCTCCGACACTCTCCACCATCGTACCCCATCGCTCCGGCGATCTTTACTCCAAACAATGCTTCTTATTCCTCTGTTCTTGAGACTTACATTAGAAACTTGAGATTCAACACTTCAACAACTCCTAAACCATTCCTCATTCTCACTGCTCTCCATGAATCTCATGTTAAAGTTGCTATTCTTTGTGCTCGAATCCATAATCTTCAGATGAAGATTCGTAGCGGCGGCCATGATTACGAAGGCGTTTCTTACGTCTCCGACGTCGTCCCTTTCTTCGTACTCGATATGTTCAACCTACGAGAGATAACGGTTGATGCCAAAACCGAGACCGCTTGGGTCCAAACCGGAGCAACTCTAGGAGAAGTGTATTATAGAGTTGCTGAGAAAAATAAGTCCTACGGCTTTCCCGCCGGCGTGTGCCCAACGGTGGGGGTCGGCGGTCATTTTGGTGGTGGCGGCTATGGTAACATGATGAGAAAACACGGCCTCTCGGTAGATCAAATAATCGATGCAAAGATTATCGACGTCAATGGAAAACTCCTTGACCGAAAGGCAATGGGGGAGGATCTATTTTGGGCCATTATAGGCGGTGGAGGATCTAGCTTCGGTGTGGTGGTAGCATACAAAATCCGAATGGTTCGTGTGCCGGAGAGGGTAACGGTGTTTCGAGTACAAAGAACATTGGAACAAAATGAGTTAACGGAGATTGTAGATGAGTGGCAACAGGTGGCACATGTGATTGACAATGATTTGTTCATAAGAGTAACGTTTGACGTTGTGAATGGAACAGACAAGGAAAAAAAGACATTGAGAGCTACGTTTATAGCCTTATTCCTAGGAGATTCCGGAAGGCTTTTGTTTGTCATCAACAACAGTTTTCCTAAACTTGGTTTGAAGAAATCTGATTGCATTGAAATGAGTTGGCTTCAATCTGTGCTCTTTTGGACTAATTTCCCTCCAGGCACATCAGTGGAAGCCCTACTATCCAGAACCCCACAAGTACTGACCCATCTAAAAAGAAAGTCAGATTATGTAAAAGCCCCAATTCCAAAGGAAGGACTCAACAAAATATGGAAGAAAATGATAGAACTTGAAAAACCCATGATGACATTTAACCCTTATGGTGGAAGAATGGCAGAAATTCCATCGAATGCAACTCCATTTCCTCATAGAGCTGGAAACTTATGGAAGATTCAATATGCAACAAATTGGGATGAAGAAGGAAAAAGAGAAGCCAAACATTTTATTGATTTGACAAGGAAACTCTACAAGTTTATGACACCATTTGTGTCAAAAAATCCCAGAACTGCCTTTTTGAACTATAGAGATCTTGATATTGGTGTAAATCACAATGGGAAGAATAGTTACTATGAAGGAAGAGTTTATGGCATTAAATACTTTGAAGGTAACTTTGAGAGGTTGGTGAAGATCAAGACCAAGGTTGATCCTCACAACTTTTTTAGGAATGAACAAAGCATTCCAAGGTTTCCTCATGTTTGAAGATAGAAAATGATTGATTGTGTGTTATTGTTTCTCTTTTGTTGTTTTCTTTGTTCATTGTCATTTCAAGATTAACTTTTTCTTTTCTAAATAATATAAAGTGTGTAAGTTATATATAGTCGTTATATATTATATTATATTATATAGTTGTATATGTTGGATAGATGTGATGATTAGATTATAAAGCCATTACTGATAATGTATCTTTATGTACGTGAAAATGAAAGGGCTCCTTCTAAGTTTATCACATATTCAAATTAATTAGATGT

Coding sequence (CDS)

ATGCAAATTTTAAGGTCTTCAATTTTGTTCTTATCCTTTTCATTCCTTGTGTTTTTTTCTTCTGCATCTTCTTCAGCTCCTGAAAATTTCTTGCAATGTCTTCTCCGACACTCTCCACCATCGTACCCCATCGCTCCGGCGATCTTTACTCCAAACAATGCTTCTTATTCCTCTGTTCTTGAGACTTACATTAGAAACTTGAGATTCAACACTTCAACAACTCCTAAACCATTCCTCATTCTCACTGCTCTCCATGAATCTCATGTTAAAGTTGCTATTCTTTGTGCTCGAATCCATAATCTTCAGATGAAGATTCGTAGCGGCGGCCATGATTACGAAGGCGTTTCTTACGTCTCCGACGTCGTCCCTTTCTTCGTACTCGATATGTTCAACCTACGAGAGATAACGGTTGATGCCAAAACCGAGACCGCTTGGGTCCAAACCGGAGCAACTCTAGGAGAAGTGTATTATAGAGTTGCTGAGAAAAATAAGTCCTACGGCTTTCCCGCCGGCGTGTGCCCAACGGTGGGGGTCGGCGGTCATTTTGGTGGTGGCGGCTATGGTAACATGATGAGAAAACACGGCCTCTCGGTAGATCAAATAATCGATGCAAAGATTATCGACGTCAATGGAAAACTCCTTGACCGAAAGGCAATGGGGGAGGATCTATTTTGGGCCATTATAGGCGGTGGAGGATCTAGCTTCGGTGTGGTGGTAGCATACAAAATCCGAATGGTTCGTGTGCCGGAGAGGGTAACGGTGTTTCGAGTACAAAGAACATTGGAACAAAATGAGTTAACGGAGATTGTAGATGAGTGGCAACAGGTGGCACATGTGATTGACAATGATTTGTTCATAAGAGTAACGTTTGACGTTGTGAATGGAACAGACAAGGAAAAAAAGACATTGAGAGCTACGTTTATAGCCTTATTCCTAGGAGATTCCGGAAGGCTTTTGTTTGTCATCAACAACAGTTTTCCTAAACTTGGTTTGAAGAAATCTGATTGCATTGAAATGAGTTGGCTTCAATCTGTGCTCTTTTGGACTAATTTCCCTCCAGGCACATCAGTGGAAGCCCTACTATCCAGAACCCCACAAGTACTGACCCATCTAAAAAGAAAGTCAGATTATGTAAAAGCCCCAATTCCAAAGGAAGGACTCAACAAAATATGGAAGAAAATGATAGAACTTGAAAAACCCATGATGACATTTAACCCTTATGGTGGAAGAATGGCAGAAATTCCATCGAATGCAACTCCATTTCCTCATAGAGCTGGAAACTTATGGAAGATTCAATATGCAACAAATTGGGATGAAGAAGGAAAAAGAGAAGCCAAACATTTTATTGATTTGACAAGGAAACTCTACAAGTTTATGACACCATTTGTGTCAAAAAATCCCAGAACTGCCTTTTTGAACTATAGAGATCTTGATATTGGTGTAAATCACAATGGGAAGAATAGTTACTATGAAGGAAGAGTTTATGGCATTAAATACTTTGAAGGTAACTTTGAGAGGTTGGTGAAGATCAAGACCAAGGTTGATCCTCACAACTTTTTTAGGAATGAACAAAGCATTCCAAGGTTTCCTCATGTTTGA

Protein sequence

MQILRSSILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVLETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSDVVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGGHFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEKKTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPPGTSVEALLSRTPQVLTHLKRKSDYVKAPIPKEGLNKIWKKMIELEKPMMTFNPYGGRMAEIPSNATPFPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIGVNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV
Homology
BLAST of Cmc12g0327701 vs. NCBI nr
Match: XP_008445642.1 (PREDICTED: reticuline oxidase-like protein [Cucumis melo] >KAA0047124.1 reticuline oxidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1071.2 bits (2769), Expect = 2.9e-309
Identity = 530/532 (99.62%), Postives = 531/532 (99.81%), Query Frame = 0

Query: 1   MQILRSSILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60
           MQILRSSILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVL
Sbjct: 1   MQILRSSILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60

Query: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSD 120
           ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSD
Sbjct: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSD 120

Query: 121 VVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180
           VVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG
Sbjct: 121 VVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180

Query: 181 HFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240
           HFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 181 HFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240

Query: 241 YKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEK 300
           YKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEK
Sbjct: 241 YKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEK 300

Query: 301 KTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPPGTSVEAL 360
           KTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFP GTSVEAL
Sbjct: 301 KTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360

Query: 361 LSRTPQVLTHLKRKSDYVKAPIPKEGLNKIWKKMIELEKPMMTFNPYGGRMAEIPSNATP 420
           LSRTPQVLTHLKRKSDYVKAPIPKEGL+KIWKKMIELEKPMMTFNPYGGRMAEIPSNATP
Sbjct: 361 LSRTPQVLTHLKRKSDYVKAPIPKEGLDKIWKKMIELEKPMMTFNPYGGRMAEIPSNATP 420

Query: 421 FPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG
Sbjct: 421 FPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480

Query: 481 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV 533
           VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV
Sbjct: 481 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV 532

BLAST of Cmc12g0327701 vs. NCBI nr
Match: XP_004135723.1 (berberine bridge enzyme-like 8 [Cucumis sativus] >KAE8653304.1 hypothetical protein Csa_023313 [Cucumis sativus])

HSP 1 Score: 1035.0 bits (2675), Expect = 2.2e-298
Identity = 510/532 (95.86%), Postives = 520/532 (97.74%), Query Frame = 0

Query: 1   MQILRSSILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60
           M+ILR+SIL LSFSFLVFFSSASSSAPENF QCLLRHSPPSYPIAPAIFTPNNASYSSVL
Sbjct: 1   MEILRTSILLLSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60

Query: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSD 120
           ETYIRNLRFNTSTTPKPFLILTALHESHVK AILCARIHNLQMKIRSGGHDYEGVSYVSD
Sbjct: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSD 120

Query: 121 VVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180
           VVPFF+LDMFNLREITVDAKT+TAWVQTGATLGEVYYRVAE NKSYGFPAGVCPTVGVGG
Sbjct: 121 VVPFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGG 180

Query: 181 HFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240
           HFGGGGYGNMMRK+GLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240

Query: 241 YKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEK 300
           YKIRMV VPE VTVFRVQRTLEQN+LTEIVDEWQQVAHVIDNDLFIRVTFDVVNGT+K K
Sbjct: 241 YKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK 300

Query: 301 KTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPPGTSVEAL 360
           KTLRATFIALFLGDS RLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFP GTSVEAL
Sbjct: 301 KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360

Query: 361 LSRTPQVLTHLKRKSDYVKAPIPKEGLNKIWKKMIELEKPMMTFNPYGGRMAEIPSNATP 420
           LSRTPQVLTHLKRKSDYVK PIPKEGLNKIWKKMIELEKPM+TFNPYGGRMAEIPSNATP
Sbjct: 361 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 420

Query: 421 FPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNW EEG +EAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG
Sbjct: 421 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480

Query: 481 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV 533
           VNHNGKNSYYEGRVYGIKYFEGNF+RLVKIKTKVDPHNFFRNEQSIPRFPHV
Sbjct: 481 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHV 532

BLAST of Cmc12g0327701 vs. NCBI nr
Match: XP_038895093.1 (berberine bridge enzyme-like 8 [Benincasa hispida])

HSP 1 Score: 1009.6 bits (2609), Expect = 1.0e-290
Identity = 494/532 (92.86%), Postives = 516/532 (96.99%), Query Frame = 0

Query: 1   MQILRSSILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60
           M+ILRSSIL LSFS+LVFFSSA +SAPENF QCLLRHSPPSYPIAPAIFTPNN S+SSVL
Sbjct: 1   MEILRSSILLLSFSYLVFFSSA-TSAPENFFQCLLRHSPPSYPIAPAIFTPNNVSFSSVL 60

Query: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSD 120
           ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIH+LQMKIRSGGHDYEGVSYVSD
Sbjct: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVSD 120

Query: 121 VVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180
           V+PFF+LDMFNLR+ITVD KT+TAWVQTGATLGEVYYR+AEKNK YGFPAGVCPTVGVGG
Sbjct: 121 VLPFFILDMFNLRDITVDVKTKTAWVQTGATLGEVYYRIAEKNKFYGFPAGVCPTVGVGG 180

Query: 181 HFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240
           HFGGGGYGNMMRK+GLSVDQIIDAKI+DVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240

Query: 241 YKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEK 300
           YKIRMVRVPE VTVFRVQRTLEQN+LTEIVDEWQ+VAHVIDNDLFIRVTFDV+NGT+K K
Sbjct: 241 YKIRMVRVPETVTVFRVQRTLEQNDLTEIVDEWQRVAHVIDNDLFIRVTFDVINGTEKGK 300

Query: 301 KTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPPGTSVEAL 360
           KTLRATF+A+FLGDS RLL VI  SFPKLGLKKSDC+EMSWLQSVLFWTNFP GTSV+AL
Sbjct: 301 KTLRATFLAMFLGDSERLLSVIKKSFPKLGLKKSDCLEMSWLQSVLFWTNFPLGTSVDAL 360

Query: 361 LSRTPQVLTHLKRKSDYVKAPIPKEGLNKIWKKMIELEKPMMTFNPYGGRMAEIPSNATP 420
           LSRTPQVLTHLKRKSDYVK PIPKEGLNKIWKKMIELEKPM+TFNPYGGRMAEIPSNATP
Sbjct: 361 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 420

Query: 421 FPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNWDEEG +EAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG
Sbjct: 421 FPHRAGNLWKIQYATNWDEEGTQEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480

Query: 481 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV 533
           VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDP NFFRNEQSIPRFPHV
Sbjct: 481 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPDNFFRNEQSIPRFPHV 531

BLAST of Cmc12g0327701 vs. NCBI nr
Match: XP_022994779.1 (berberine bridge enzyme-like 8 [Cucurbita maxima])

HSP 1 Score: 957.2 bits (2473), Expect = 5.9e-275
Identity = 472/532 (88.72%), Postives = 500/532 (93.98%), Query Frame = 0

Query: 1   MQILRSSILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60
           M+I R SI  LSFS L+FF+SA ++APENFLQCLLRHSPPSYPIAPAIFT NNASYSSVL
Sbjct: 3   MEISRFSIFLLSFSALLFFASA-TTAPENFLQCLLRHSPPSYPIAPAIFTSNNASYSSVL 62

Query: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSD 120
           ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIH+LQMKIRSGGHDYEGVSYVSD
Sbjct: 63  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVSD 122

Query: 121 VVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180
           VVPFFVLDMFNLR+I+VD KTETAWVQTGATLGEVYYR+AEK K +GFPAGVCPTVGVGG
Sbjct: 123 VVPFFVLDMFNLRDISVDVKTETAWVQTGATLGEVYYRIAEKIKFHGFPAGVCPTVGVGG 182

Query: 181 HFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240
           HFGGGGYGNMMRK+GLSVDQIIDAKI+DV G LLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 183 HFGGGGYGNMMRKYGLSVDQIIDAKIVDVYGNLLDRKAMGEDLFWAIIGGGGSSFGVVVA 242

Query: 241 YKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEK 300
           YKIR VRVPE VTVFRV RTLE N++TEIVD+WQ+VAHVID DLFIRVTFDVVNG+   K
Sbjct: 243 YKIRTVRVPETVTVFRVGRTLEDNDVTEIVDQWQRVAHVIDKDLFIRVTFDVVNGSRSGK 302

Query: 301 KTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPPGTSVEAL 360
           KTLRATFIALFLGDS RLL V+N SFPKLGLKKSDC+EMSWLQSVLFWTNFP GTSV+ L
Sbjct: 303 KTLRATFIALFLGDSERLLSVMNKSFPKLGLKKSDCLEMSWLQSVLFWTNFPQGTSVDEL 362

Query: 361 LSRTPQVLTHLKRKSDYVKAPIPKEGLNKIWKKMIELEKPMMTFNPYGGRMAEIPSNATP 420
           LSRTPQVLTHLKRKSDYVK  IPKE LN+IWKKMIELEKP MTFNPYGGRMAEIPSNATP
Sbjct: 363 LSRTPQVLTHLKRKSDYVKTLIPKEKLNQIWKKMIELEKPTMTFNPYGGRMAEIPSNATP 422

Query: 421 FPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNWDE+G ++A HFIDLTRKLYKFMTPFV+KNPRTAFLNYRDLDIG
Sbjct: 423 FPHRAGNLWKIQYATNWDEQGTQQANHFIDLTRKLYKFMTPFVAKNPRTAFLNYRDLDIG 482

Query: 481 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV 533
           VNHNGK SYYEGRV+GIKYFEGNF+RLVKIKTKVDPHNFFRNEQSIPRFP+V
Sbjct: 483 VNHNGKRSYYEGRVFGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPYV 533

BLAST of Cmc12g0327701 vs. NCBI nr
Match: KAG6573419.1 (Berberine bridge enzyme-like 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 956.1 bits (2470), Expect = 1.3e-274
Identity = 472/532 (88.72%), Postives = 499/532 (93.80%), Query Frame = 0

Query: 1   MQILRSSILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60
           M+ILR S   LSFS L+FF+SA ++APENFLQCLLRHSPPSYPIAPAIFT NNASYSSVL
Sbjct: 3   MEILRFSSFLLSFSALLFFASA-TTAPENFLQCLLRHSPPSYPIAPAIFTSNNASYSSVL 62

Query: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSD 120
           ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIH+LQMKIRSGGHDYEGVSYVSD
Sbjct: 63  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVSD 122

Query: 121 VVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180
           VVPFFVLDMFNLR+I+VD KTETAWVQTGATLGEVYYR+AEK K +GFPAGVCPTVGVGG
Sbjct: 123 VVPFFVLDMFNLRDISVDVKTETAWVQTGATLGEVYYRIAEKIKFHGFPAGVCPTVGVGG 182

Query: 181 HFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240
           HFGGGGYGNMMRK+GLSVDQIIDAKI+DVNG LLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 183 HFGGGGYGNMMRKYGLSVDQIIDAKIVDVNGNLLDRKAMGEDLFWAIIGGGGSSFGVVVA 242

Query: 241 YKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEK 300
           YKIR VRVPE VTVFRV RTLE N++TEIVD+WQ+VAHVID DLFIRVTFDVVNG    K
Sbjct: 243 YKIRTVRVPETVTVFRVGRTLEDNDVTEIVDQWQRVAHVIDKDLFIRVTFDVVNGRRSGK 302

Query: 301 KTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPPGTSVEAL 360
           KTLRATFIALFLGDS RLL V+N SFPKLGLKKSDC+EMSWLQSVLFWTNFP GTSV+ L
Sbjct: 303 KTLRATFIALFLGDSERLLSVMNKSFPKLGLKKSDCLEMSWLQSVLFWTNFPQGTSVDEL 362

Query: 361 LSRTPQVLTHLKRKSDYVKAPIPKEGLNKIWKKMIELEKPMMTFNPYGGRMAEIPSNATP 420
           LSRTPQVLTHLKRKSDYVK  IPKE LN+IWKKMIELEKP MTFNPYGGRMAEIPSNATP
Sbjct: 363 LSRTPQVLTHLKRKSDYVKTLIPKENLNQIWKKMIELEKPTMTFNPYGGRMAEIPSNATP 422

Query: 421 FPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNWDE+G ++A HFIDLTRKLYKFMTPFV+KNPRTAFLNYRDLDIG
Sbjct: 423 FPHRAGNLWKIQYATNWDEQGTQQANHFIDLTRKLYKFMTPFVAKNPRTAFLNYRDLDIG 482

Query: 481 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV 533
           VNHNGK SYYEGRV+GIKYFEGNF+RLVKIKTKVDP NFFRNEQSIPRFP+V
Sbjct: 483 VNHNGKRSYYEGRVFGIKYFEGNFDRLVKIKTKVDPLNFFRNEQSIPRFPYV 533

BLAST of Cmc12g0327701 vs. ExPASy Swiss-Prot
Match: Q9SA85 (Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2 SV=1)

HSP 1 Score: 683.7 bits (1763), Expect = 1.6e-195
Identity = 340/526 (64.64%), Postives = 409/526 (77.76%), Query Frame = 0

Query: 8   ILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVLETYIRNL 67
           IL L F   ++ SS+SS+  E F QCL  +S P +PI+PAIF   N SYSSVL+  IRNL
Sbjct: 6   ILVLFFVVFIWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQANIRNL 65

Query: 68  RFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVS-DVVPFFV 127
           RFNT++TPKPFLI+ A HESHV+ AI C + HNLQMKIRSGGHDY+G+SYV+    PFFV
Sbjct: 66  RFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPFFV 125

Query: 128 LDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGGHFGGGG 187
           LDMFNLR + VD  ++TAWVQTGA LGEVYY + EK+K+  +PAG+CPTVGVGGH  GGG
Sbjct: 126 LDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISGGG 185

Query: 188 YGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIRMV 247
           YGNMMRK+GL+VD  IDA+++DVNGK+LDRK MGEDL+WAI GGGG S+GVV+AYKI +V
Sbjct: 186 YGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKINLV 245

Query: 248 RVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEKKTLRAT 307
            VPE VTVFR+ RTLEQN  T+I+  WQQVA  + ++LFIR   DVVNGT   +KT+R T
Sbjct: 246 EVPENVTVFRISRTLEQN-ATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVRTT 305

Query: 308 FIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPPGTSVEALLSRTPQ 367
           FIA+FLGD+  LL ++N  FP+LGL +SDC E SW+QSVLFWTN   G+S E LL +  Q
Sbjct: 306 FIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSS-ETLLLQRNQ 365

Query: 368 VLTHLKRKSDYVKAPIPKEGLNKIWKKMIELEKPMMTFNPYGGRMAEIPSNATPFPHRAG 427
            + +LKRKSDYV+ PI + GL  IWKKMIELE P M FNPYGG M  I S  TPFP+RAG
Sbjct: 366 PVNYLKRKSDYVREPISRTGLESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPFPYRAG 425

Query: 428 NLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIGVN-HNG 487
           NLWKIQY  NW +E   +   +++LTRKLY+FMTPFVSKNPR +F NYRD+D+G+N HNG
Sbjct: 426 NLWKIQYGANWRDETLTD--RYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHNG 485

Query: 488 K-NSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFP 531
           K +SY EG+ YG KYF GNFERLVKIKT+VD  NFFRNEQSIP  P
Sbjct: 486 KISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVLP 527

BLAST of Cmc12g0327701 vs. ExPASy Swiss-Prot
Match: Q9SVG3 (Berberine bridge enzyme-like 21 OS=Arabidopsis thaliana OX=3702 GN=At4g20840 PE=2 SV=1)

HSP 1 Score: 569.7 bits (1467), Expect = 3.5e-161
Identity = 295/531 (55.56%), Postives = 377/531 (71.00%), Query Frame = 0

Query: 7   SILFLSFSFLV---FFSSA----SSSAPENFLQCLL-RHSPPSYPIAPAIFTPNNASYSS 66
           S+ F  F FLV   FFSSA    S S  E+F+QC   +   P   I   +F+  N S+SS
Sbjct: 9   SVFFFVF-FLVSLPFFSSAAPPSSDSIYESFVQCFSDKTKSPQAQITDNVFSRTNPSFSS 68

Query: 67  VLETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYV 126
           VL  YIRN RFNTS+TPKP +I+T   + HV  A+ C++  N  +KIRSGGHDYEG+SY+
Sbjct: 69  VLRAYIRNGRFNTSSTPKPAIIVTPRSDIHVSAAVTCSKSLNFLLKIRSGGHDYEGLSYI 128

Query: 127 SDVVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGV 186
           SD  PFF+LDM NLR+++VD   ++AW+  GATLGEVYYR+ EK+K +GFPAGVCPTVGV
Sbjct: 129 SD-KPFFILDMSNLRDVSVDIADQSAWISAGATLGEVYYRIWEKSKVHGFPAGVCPTVGV 188

Query: 187 GGHFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVV 246
           GGH  GGGYGNM+RK GLSVD +IDAKI+DVNG++LDRK+MGEDLFWAI GGGG+SFGVV
Sbjct: 189 GGHISGGGYGNMLRKFGLSVDNLIDAKIVDVNGQILDRKSMGEDLFWAISGGGGASFGVV 248

Query: 247 VAYKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDK 306
           + YK+++V VPE VTVFRV++ ++   + ++V +WQ V    D +LF+R+    V  T K
Sbjct: 249 LGYKVKLVPVPETVTVFRVEKYMDSGAV-DMVHKWQSVGPKTDRNLFLRMLIQPV--TRK 308

Query: 307 EKKTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPPGTSVE 366
           + KT+RAT +ALFLG +  ++ ++   FP+L LKK +C EM+W QS L+W N    T ++
Sbjct: 309 KVKTVRATVVALFLGRAEEVVALLGKEFPELSLKKENCSEMTWFQSALWWDNRVNPTQID 368

Query: 367 --ALLSRTPQVLTHLKRKSDYVKAPIPKEGLNKIWKKMIELEKPMMTFNPYGGRMAEIPS 426
               L R        KRKSDYV + IP++G+  ++KKM EL K  + FNPYGG+MAE+  
Sbjct: 369 PKVFLDRNLDRANFGKRKSDYVASEIPRDGIESLFKKMTELGKIGLVFNPYGGKMAEVTV 428

Query: 427 NATPFPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRD 486
           NATPFPHR+  L+KIQY+  W E      K F++    LY FMT FVSKNPR A+LNYRD
Sbjct: 429 NATPFPHRS-KLFKIQYSVTWQENSVEIEKGFLNQANVLYSFMTGFVSKNPRNAYLNYRD 488

Query: 487 LDIGVNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIP 528
           +DIGVN +G NSY EG VYG KYF  NF+RLVK+KT  DP NFFRNEQSIP
Sbjct: 489 VDIGVNDHGTNSYEEGEVYGRKYFGDNFDRLVKVKTAADPDNFFRNEQSIP 533

BLAST of Cmc12g0327701 vs. ExPASy Swiss-Prot
Match: Q9SA99 (Berberine bridge enzyme-like 2 OS=Arabidopsis thaliana OX=3702 GN=At1g11770 PE=2 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 3.2e-159
Identity = 289/530 (54.53%), Postives = 372/530 (70.19%), Query Frame = 0

Query: 8   ILFLSFSFLVFFSSASSSAPE----NFLQCLLRHS-PPSYPIAPAIFTPNNASYSSVLET 67
           ILFL  SF +  S A    PE    NFLQC    +  P   +A  +     A+++ VL  
Sbjct: 7   ILFLISSF-ISTSLAVEPPPETIYQNFLQCFTNQTKAPPNSLADVVLPKTAAAFTPVLRA 66

Query: 68  YIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSDVV 127
           YIRN RFNT+ TPKP +++ A  ESHV+ A++C +  N+Q+K RSGGHDYEGVSY+S  V
Sbjct: 67  YIRNARFNTTATPKPAIVIAARSESHVQAAVICTKSLNIQLKTRSGGHDYEGVSYISH-V 126

Query: 128 PFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGGHF 187
           PFFVLDM NLR ITVD  TE+AWV  GATLGEVYYR+ EK KS+GFPAGVCPTVG GGH 
Sbjct: 127 PFFVLDMSNLRNITVDPATESAWVGAGATLGEVYYRIWEKTKSHGFPAGVCPTVGAGGHI 186

Query: 188 GGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYK 247
            GGGYGNM+RK+GLSVD + DAKI+DVNG++LDRK MGED+FWAI GGGG+SFGV++A+K
Sbjct: 187 SGGGYGNMIRKYGLSVDYVTDAKIVDVNGQVLDRKGMGEDMFWAINGGGGASFGVILAFK 246

Query: 248 IRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEKKT 307
           I++V VP  VTVFRV++ L +N  TE+V +WQ VA   D  LF+R+    V  T  + +T
Sbjct: 247 IKLVPVPPTVTVFRVEKNLVEN-ATEMVHKWQFVAPKTDPGLFMRLLLQPV--TRNKMQT 306

Query: 308 LRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPPGTSV--EAL 367
           +RA+ +ALFLGD   ++ ++   FP+LGLKK +C EM+W+QSV++W N    T +  E L
Sbjct: 307 VRASVVALFLGDQNTVMSMLTKEFPELGLKKENCTEMTWIQSVMWWANNDNATQIKPEIL 366

Query: 368 LSRTPQVLTHLKRKSDYVKAPIPKEGLNKIWKKMIELEKPMMTFNPYGGRMAEIPSNATP 427
           L R P + T  KRKSD+V+  I K+GL+ ++KKMIE+ K  + FNPYGG M+ + +  TP
Sbjct: 367 LDRNPDMATFGKRKSDFVEKEITKDGLDFLFKKMIEVGKIGLVFNPYGGIMSTVATTKTP 426

Query: 428 FPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 487
           FPHR   L+KIQ++ NW + G      F+   +  Y +M PFV+KNPR  ++NYRDLDIG
Sbjct: 427 FPHRK-KLYKIQHSMNWKDPGTEAETSFLQKAKSFYSYMAPFVTKNPRHTYINYRDLDIG 486

Query: 488 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFP 531
           VN  G NSY    V+G  YF  NF+RLVK+KT VDP NFFR+EQSIP  P
Sbjct: 487 VNTPGPNSYRVAEVFGRMYFGENFDRLVKVKTAVDPQNFFRDEQSIPTLP 530

BLAST of Cmc12g0327701 vs. ExPASy Swiss-Prot
Match: Q9SVG4 (Berberine bridge enzyme-like 19 OS=Arabidopsis thaliana OX=3702 GN=At4g20830 PE=1 SV=2)

HSP 1 Score: 561.6 bits (1446), Expect = 9.4e-159
Identity = 286/523 (54.68%), Postives = 373/523 (71.32%), Query Frame = 0

Query: 8   ILFLSFSFLVFFSSASSSAPENFLQCLL-RHSPPSYPIAPAIFTPNNASYSSVLETYIRN 67
           +LFLS     F  S S+S   +FL+C   +   P   I   +F+  N ++SSVL  YIRN
Sbjct: 19  LLFLSLPLSSF--SQSNSVYNSFLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLRAYIRN 78

Query: 68  LRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSDVVPFFV 127
            RFNTS+T KP +I+T   ESHV  A+ C++  N  +KIRSGGHDY+G+SY+SD  PFF+
Sbjct: 79  ARFNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISD-KPFFI 138

Query: 128 LDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGGHFGGGG 187
           LDM N+R+++VD  + +AW+  GATLGEVYYR+ EK++ +GFPAGVCPTVGVGGH  GGG
Sbjct: 139 LDMSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHLSGGG 198

Query: 188 YGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIRMV 247
           YGNM+RK GLSVD + DAKI+DVNG++LDRKAMGEDLFWAI GGGG S+GVV+ YK+++V
Sbjct: 199 YGNMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYKVKLV 258

Query: 248 RVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEKKTLRAT 307
            VP  VTVFRV++ ++   + ++V +WQ V    D +LF+R+    V  T K+ KT+RA+
Sbjct: 259 PVPSVVTVFRVEQYMDSGAV-DMVHKWQSVGPKTDPNLFMRMLIQPV--TRKKVKTVRAS 318

Query: 308 FIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPPGTSVE--ALLSRT 367
            +ALFLG +  ++ +++  FP+LGLKK +C EM+W QS L+W N    T V+    L R 
Sbjct: 319 VVALFLGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDRN 378

Query: 368 PQVLTHLKRKSDYVKAPIPKEGLNKIWKKMIELEKPMMTFNPYGGRMAEIPSNATPFPHR 427
               +  KRKSDYV   IPK+G+  ++KKMIEL K  + FNPYGG+MAE+  NA PFPHR
Sbjct: 379 LDTSSFGKRKSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPHR 438

Query: 428 AGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIGVNHN 487
              L+KIQY+ NW E      K +++  + LY FMT FVSKNPR+++ NYRD+DIGVN +
Sbjct: 439 -NKLFKIQYSVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVNDH 498

Query: 488 GKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIP 528
           G NSY EG VYG KYF  NF+RLVKIKT VDP NFFRNEQSIP
Sbjct: 499 GANSYKEGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIP 534

BLAST of Cmc12g0327701 vs. ExPASy Swiss-Prot
Match: F4HV09 (Berberine bridge enzyme-like 14 OS=Arabidopsis thaliana OX=3702 GN=At1g34575 PE=3 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 1.0e-157
Identity = 280/525 (53.33%), Postives = 380/525 (72.38%), Query Frame = 0

Query: 7   SILFLSFSFLVFFS-SASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVLETYIR 66
           +++F  F  L+  S +A     ++F QC+     PS PI    +T  N ++ ++L  Y+R
Sbjct: 8   TLIFTVFLLLIPTSFAAPPKLKDSFTQCVTVFK-PSVPIQNFTYTQQNPNFLTILNNYVR 67

Query: 67  NLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSDVVPFF 126
           NLR+   TT KP  I+ A H +H++  I CA+   LQ++IRSGGHDY+G+SY+S  V F 
Sbjct: 68  NLRYFNGTTRKPVAIVAAAHFTHIQATINCAKKLGLQLRIRSGGHDYDGMSYLS-TVDFV 127

Query: 127 VLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEK-NKSYGFPAGVCPTVGVGGHFGG 186
           VLDMFNLR I +D K +TAWVQ+GATLGE+YY VA K N   GFPAG+CP +G GGHF G
Sbjct: 128 VLDMFNLRAIEIDPKLDTAWVQSGATLGEIYYNVANKSNNLRGFPAGICPGLGAGGHFSG 187

Query: 187 GGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIR 246
           GGYGNMMRK+GLS+D IIDAKI+D N ++LDR +MGEDLFWA+ GGG +SF VV+A+KI+
Sbjct: 188 GGYGNMMRKYGLSIDNIIDAKIVDANARVLDRSSMGEDLFWALRGGGAASFCVVLAWKIK 247

Query: 247 MVRVPERVTVFRVQRTLEQNEL-TEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEKKTL 306
           +V VPE+VTVF V+    +  + T++  +WQ++A  IDNDLFIR+T    N      KT+
Sbjct: 248 LVPVPEKVTVFNVETIGNRGVIPTDLAAKWQEIADKIDNDLFIRLTLSSSN------KTV 307

Query: 307 RATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPPGTS-VEALLS 366
           +A+F+ ++LG+S +LL ++N  FP+LGL K++CIEM W++SVLFW + PPGT+    +L+
Sbjct: 308 KASFMGMYLGNSEKLLEIMNAKFPELGLNKTECIEMKWIESVLFWLSIPPGTAPTSVMLN 367

Query: 367 RTPQVLTHLKRKSDYVKAPIPKEGLNKIWKKMIELEKPMMTFNPYGGRMAEIPSNATPFP 426
           R PQ   +LKRKSDYV+ PI K GL  I+K + E E   M +NPYGGRM+EIP+  T FP
Sbjct: 368 RIPQKQIYLKRKSDYVQKPISKPGLESIFKILSENENVSMAWNPYGGRMSEIPATETAFP 427

Query: 427 HRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIGVN 486
           HRAGN++KIQY++NW   G+  A   +  T ++++ M+P+VSKNPR AFLNYRD+DIG N
Sbjct: 428 HRAGNMFKIQYSSNWFVPGEEAASDCLSQTERVFEAMSPYVSKNPREAFLNYRDIDIGKN 487

Query: 487 HNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIP 528
            N  ++Y EG+VYG+KYF+ NFERLV++KT+VDP N FR EQSIP
Sbjct: 488 LN--STYEEGKVYGVKYFKNNFERLVQVKTRVDPDNIFRYEQSIP 522

BLAST of Cmc12g0327701 vs. ExPASy TrEMBL
Match: A0A5A7TVZ2 (Reticuline oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold466G001070 PE=3 SV=1)

HSP 1 Score: 1071.2 bits (2769), Expect = 1.4e-309
Identity = 530/532 (99.62%), Postives = 531/532 (99.81%), Query Frame = 0

Query: 1   MQILRSSILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60
           MQILRSSILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVL
Sbjct: 1   MQILRSSILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60

Query: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSD 120
           ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSD
Sbjct: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSD 120

Query: 121 VVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180
           VVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG
Sbjct: 121 VVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180

Query: 181 HFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240
           HFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 181 HFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240

Query: 241 YKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEK 300
           YKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEK
Sbjct: 241 YKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEK 300

Query: 301 KTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPPGTSVEAL 360
           KTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFP GTSVEAL
Sbjct: 301 KTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360

Query: 361 LSRTPQVLTHLKRKSDYVKAPIPKEGLNKIWKKMIELEKPMMTFNPYGGRMAEIPSNATP 420
           LSRTPQVLTHLKRKSDYVKAPIPKEGL+KIWKKMIELEKPMMTFNPYGGRMAEIPSNATP
Sbjct: 361 LSRTPQVLTHLKRKSDYVKAPIPKEGLDKIWKKMIELEKPMMTFNPYGGRMAEIPSNATP 420

Query: 421 FPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG
Sbjct: 421 FPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480

Query: 481 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV 533
           VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV
Sbjct: 481 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV 532

BLAST of Cmc12g0327701 vs. ExPASy TrEMBL
Match: A0A1S3BE21 (reticuline oxidase-like protein OS=Cucumis melo OX=3656 GN=LOC103488597 PE=3 SV=1)

HSP 1 Score: 1071.2 bits (2769), Expect = 1.4e-309
Identity = 530/532 (99.62%), Postives = 531/532 (99.81%), Query Frame = 0

Query: 1   MQILRSSILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60
           MQILRSSILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVL
Sbjct: 1   MQILRSSILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60

Query: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSD 120
           ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSD
Sbjct: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSD 120

Query: 121 VVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180
           VVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG
Sbjct: 121 VVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180

Query: 181 HFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240
           HFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 181 HFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240

Query: 241 YKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEK 300
           YKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEK
Sbjct: 241 YKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEK 300

Query: 301 KTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPPGTSVEAL 360
           KTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFP GTSVEAL
Sbjct: 301 KTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360

Query: 361 LSRTPQVLTHLKRKSDYVKAPIPKEGLNKIWKKMIELEKPMMTFNPYGGRMAEIPSNATP 420
           LSRTPQVLTHLKRKSDYVKAPIPKEGL+KIWKKMIELEKPMMTFNPYGGRMAEIPSNATP
Sbjct: 361 LSRTPQVLTHLKRKSDYVKAPIPKEGLDKIWKKMIELEKPMMTFNPYGGRMAEIPSNATP 420

Query: 421 FPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG
Sbjct: 421 FPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480

Query: 481 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV 533
           VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV
Sbjct: 481 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV 532

BLAST of Cmc12g0327701 vs. ExPASy TrEMBL
Match: A0A6J1K3U3 (berberine bridge enzyme-like 8 OS=Cucurbita maxima OX=3661 GN=LOC111490407 PE=3 SV=1)

HSP 1 Score: 957.2 bits (2473), Expect = 2.8e-275
Identity = 472/532 (88.72%), Postives = 500/532 (93.98%), Query Frame = 0

Query: 1   MQILRSSILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60
           M+I R SI  LSFS L+FF+SA ++APENFLQCLLRHSPPSYPIAPAIFT NNASYSSVL
Sbjct: 3   MEISRFSIFLLSFSALLFFASA-TTAPENFLQCLLRHSPPSYPIAPAIFTSNNASYSSVL 62

Query: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSD 120
           ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIH+LQMKIRSGGHDYEGVSYVSD
Sbjct: 63  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVSD 122

Query: 121 VVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180
           VVPFFVLDMFNLR+I+VD KTETAWVQTGATLGEVYYR+AEK K +GFPAGVCPTVGVGG
Sbjct: 123 VVPFFVLDMFNLRDISVDVKTETAWVQTGATLGEVYYRIAEKIKFHGFPAGVCPTVGVGG 182

Query: 181 HFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240
           HFGGGGYGNMMRK+GLSVDQIIDAKI+DV G LLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 183 HFGGGGYGNMMRKYGLSVDQIIDAKIVDVYGNLLDRKAMGEDLFWAIIGGGGSSFGVVVA 242

Query: 241 YKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEK 300
           YKIR VRVPE VTVFRV RTLE N++TEIVD+WQ+VAHVID DLFIRVTFDVVNG+   K
Sbjct: 243 YKIRTVRVPETVTVFRVGRTLEDNDVTEIVDQWQRVAHVIDKDLFIRVTFDVVNGSRSGK 302

Query: 301 KTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPPGTSVEAL 360
           KTLRATFIALFLGDS RLL V+N SFPKLGLKKSDC+EMSWLQSVLFWTNFP GTSV+ L
Sbjct: 303 KTLRATFIALFLGDSERLLSVMNKSFPKLGLKKSDCLEMSWLQSVLFWTNFPQGTSVDEL 362

Query: 361 LSRTPQVLTHLKRKSDYVKAPIPKEGLNKIWKKMIELEKPMMTFNPYGGRMAEIPSNATP 420
           LSRTPQVLTHLKRKSDYVK  IPKE LN+IWKKMIELEKP MTFNPYGGRMAEIPSNATP
Sbjct: 363 LSRTPQVLTHLKRKSDYVKTLIPKEKLNQIWKKMIELEKPTMTFNPYGGRMAEIPSNATP 422

Query: 421 FPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNWDE+G ++A HFIDLTRKLYKFMTPFV+KNPRTAFLNYRDLDIG
Sbjct: 423 FPHRAGNLWKIQYATNWDEQGTQQANHFIDLTRKLYKFMTPFVAKNPRTAFLNYRDLDIG 482

Query: 481 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV 533
           VNHNGK SYYEGRV+GIKYFEGNF+RLVKIKTKVDPHNFFRNEQSIPRFP+V
Sbjct: 483 VNHNGKRSYYEGRVFGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPYV 533

BLAST of Cmc12g0327701 vs. ExPASy TrEMBL
Match: A0A6J1GSQ1 (berberine bridge enzyme-like 8 OS=Cucurbita moschata OX=3662 GN=LOC111456785 PE=3 SV=1)

HSP 1 Score: 952.6 bits (2461), Expect = 7.0e-274
Identity = 470/532 (88.35%), Postives = 498/532 (93.61%), Query Frame = 0

Query: 1   MQILRSSILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60
           M I R S   LSFS L+FF+SA ++APENFLQCLLRHSPPSYPIAPAIFT NNASYSSVL
Sbjct: 3   MDISRFSSFLLSFSALLFFASA-TTAPENFLQCLLRHSPPSYPIAPAIFTSNNASYSSVL 62

Query: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSD 120
           ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIH+LQMKIRSGGHDYEGVSYVSD
Sbjct: 63  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVSD 122

Query: 121 VVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180
           VVPFFVLDMFNLR+I+VD KTETAWVQTGATLGEVYYR+AEK K +GFPAGVCPTVGVGG
Sbjct: 123 VVPFFVLDMFNLRDISVDVKTETAWVQTGATLGEVYYRIAEKIKFHGFPAGVCPTVGVGG 182

Query: 181 HFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240
           HFGGGGYGNMMRK+GLSVDQIIDAKI+DVNG LLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 183 HFGGGGYGNMMRKYGLSVDQIIDAKIVDVNGNLLDRKAMGEDLFWAIIGGGGSSFGVVVA 242

Query: 241 YKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEK 300
           YKIR VRVPE VTVFRV RTLE N++TEIVD+WQ+VAHVID DLFIRVTFDVVNG+   K
Sbjct: 243 YKIRTVRVPETVTVFRVGRTLEDNDVTEIVDQWQRVAHVIDKDLFIRVTFDVVNGSRSGK 302

Query: 301 KTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPPGTSVEAL 360
           KTLRATFIALFLGDS RLL V+N SFPKLGLKK+DC+EMSWLQSVLFWTNFP GTSV+ L
Sbjct: 303 KTLRATFIALFLGDSERLLSVMNKSFPKLGLKKADCLEMSWLQSVLFWTNFPQGTSVDEL 362

Query: 361 LSRTPQVLTHLKRKSDYVKAPIPKEGLNKIWKKMIELEKPMMTFNPYGGRMAEIPSNATP 420
           LSRTPQVLTHLKRKSDYVK  IPKE LN+IWKKMIELEKP MTFNPYGGRMAEIPSNATP
Sbjct: 363 LSRTPQVLTHLKRKSDYVKTLIPKENLNQIWKKMIELEKPTMTFNPYGGRMAEIPSNATP 422

Query: 421 FPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNWDE+G ++A HFIDLTRKLYKFMTPFV+KNPRTAFLNYRDLDIG
Sbjct: 423 FPHRAGNLWKIQYATNWDEQGTQQANHFIDLTRKLYKFMTPFVAKNPRTAFLNYRDLDIG 482

Query: 481 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV 533
           VNHNGK SYYEGRV+GIKYFEGNF+RLVKIKTKVDP NFFRNEQSIPRFP+V
Sbjct: 483 VNHNGKRSYYEGRVFGIKYFEGNFDRLVKIKTKVDPLNFFRNEQSIPRFPYV 533

BLAST of Cmc12g0327701 vs. ExPASy TrEMBL
Match: A0A6J1DGF3 (berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111020214 PE=3 SV=1)

HSP 1 Score: 912.9 bits (2358), Expect = 6.2e-262
Identity = 443/525 (84.38%), Postives = 490/525 (93.33%), Query Frame = 0

Query: 8   ILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVLETYIRNL 67
           +L L FS L F S+ ++++PENFL+CLLR SPPS+PI+PAIFTP+NAS+SSVL+TYIRNL
Sbjct: 11  LLSLLFSLLFFASATTTASPENFLKCLLRRSPPSHPISPAIFTPDNASFSSVLQTYIRNL 70

Query: 68  RFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSDVVPFFVL 127
           RFNTS+TPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSDVVPFFVL
Sbjct: 71  RFNTSSTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSDVVPFFVL 130

Query: 128 DMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGGHFGGGGY 187
           DMFNLR ITVD +TETAWV+TGATLGEVYYR+AEK+K +GFPAGVCPTVGVGGHFGGGGY
Sbjct: 131 DMFNLRAITVDVETETAWVETGATLGEVYYRIAEKSKLHGFPAGVCPTVGVGGHFGGGGY 190

Query: 188 GNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIRMVR 247
           GNMMRK+GLSVDQIIDAK++DVNGKLLDRK+MGEDLFWAI GGGGSSFG VVAYKI+ VR
Sbjct: 191 GNMMRKYGLSVDQIIDAKMVDVNGKLLDRKSMGEDLFWAITGGGGSSFGXVVAYKIQTVR 250

Query: 248 VPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEKKTLRATF 307
           VPE VTVF+V+R L +N +TEIVDEWQ+VAH IDNDLFIRVTFDVVNGT+  KKTLRATF
Sbjct: 251 VPETVTVFKVERRLAEN-VTEIVDEWQRVAHEIDNDLFIRVTFDVVNGTENGKKTLRATF 310

Query: 308 IALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPPGTSVEALLSRTPQV 367
           IALFLGDS RLL +++ SFPKLGLK+SDC++MSWL+SVLFWTNFP GTSV+ALLSR PQV
Sbjct: 311 IALFLGDSERLLSIMDKSFPKLGLKQSDCLQMSWLESVLFWTNFPLGTSVDALLSRKPQV 370

Query: 368 LTHLKRKSDYVKAPIPKEGLNKIWKKMIELEKPMMTFNPYGGRMAEIPSNATPFPHRAGN 427
           LTHLKRKSDYVK  IPKEGLN+IWKKMIELEKPM+TFNPYGGRMAEI S+ TPFPHRAGN
Sbjct: 371 LTHLKRKSDYVKTLIPKEGLNQIWKKMIELEKPMLTFNPYGGRMAEIASSKTPFPHRAGN 430

Query: 428 LWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIGVNHNGKN 487
           LWKIQYATNWDEEG  EA HFI+LTRKLY FMTPFVSKNPRTAFLNYRDLDIGVNHNGKN
Sbjct: 431 LWKIQYATNWDEEGVEEADHFINLTRKLYNFMTPFVSKNPRTAFLNYRDLDIGVNHNGKN 490

Query: 488 SYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV 533
           SY EGRV+G+KYFEGNF+RLVKIKT+VDP+NFFRNEQSIPR P+V
Sbjct: 491 SYNEGRVFGVKYFEGNFDRLVKIKTRVDPNNFFRNEQSIPRLPYV 534

BLAST of Cmc12g0327701 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 683.7 bits (1763), Expect = 1.2e-196
Identity = 340/526 (64.64%), Postives = 409/526 (77.76%), Query Frame = 0

Query: 8   ILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVLETYIRNL 67
           IL L F   ++ SS+SS+  E F QCL  +S P +PI+PAIF   N SYSSVL+  IRNL
Sbjct: 6   ILVLFFVVFIWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQANIRNL 65

Query: 68  RFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVS-DVVPFFV 127
           RFNT++TPKPFLI+ A HESHV+ AI C + HNLQMKIRSGGHDY+G+SYV+    PFFV
Sbjct: 66  RFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPFFV 125

Query: 128 LDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGGHFGGGG 187
           LDMFNLR + VD  ++TAWVQTGA LGEVYY + EK+K+  +PAG+CPTVGVGGH  GGG
Sbjct: 126 LDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISGGG 185

Query: 188 YGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIRMV 247
           YGNMMRK+GL+VD  IDA+++DVNGK+LDRK MGEDL+WAI GGGG S+GVV+AYKI +V
Sbjct: 186 YGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKINLV 245

Query: 248 RVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEKKTLRAT 307
            VPE VTVFR+ RTLEQN  T+I+  WQQVA  + ++LFIR   DVVNGT   +KT+R T
Sbjct: 246 EVPENVTVFRISRTLEQN-ATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVRTT 305

Query: 308 FIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPPGTSVEALLSRTPQ 367
           FIA+FLGD+  LL ++N  FP+LGL +SDC E SW+QSVLFWTN   G+S E LL +  Q
Sbjct: 306 FIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSS-ETLLLQRNQ 365

Query: 368 VLTHLKRKSDYVKAPIPKEGLNKIWKKMIELEKPMMTFNPYGGRMAEIPSNATPFPHRAG 427
            + +LKRKSDYV+ PI + GL  IWKKMIELE P M FNPYGG M  I S  TPFP+RAG
Sbjct: 366 PVNYLKRKSDYVREPISRTGLESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPFPYRAG 425

Query: 428 NLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIGVN-HNG 487
           NLWKIQY  NW +E   +   +++LTRKLY+FMTPFVSKNPR +F NYRD+D+G+N HNG
Sbjct: 426 NLWKIQYGANWRDETLTD--RYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHNG 485

Query: 488 K-NSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFP 531
           K +SY EG+ YG KYF GNFERLVKIKT+VD  NFFRNEQSIP  P
Sbjct: 486 KISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVLP 527

BLAST of Cmc12g0327701 vs. TAIR 10
Match: AT4G20840.1 (FAD-binding Berberine family protein )

HSP 1 Score: 569.7 bits (1467), Expect = 2.5e-162
Identity = 295/531 (55.56%), Postives = 377/531 (71.00%), Query Frame = 0

Query: 7   SILFLSFSFLV---FFSSA----SSSAPENFLQCLL-RHSPPSYPIAPAIFTPNNASYSS 66
           S+ F  F FLV   FFSSA    S S  E+F+QC   +   P   I   +F+  N S+SS
Sbjct: 9   SVFFFVF-FLVSLPFFSSAAPPSSDSIYESFVQCFSDKTKSPQAQITDNVFSRTNPSFSS 68

Query: 67  VLETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYV 126
           VL  YIRN RFNTS+TPKP +I+T   + HV  A+ C++  N  +KIRSGGHDYEG+SY+
Sbjct: 69  VLRAYIRNGRFNTSSTPKPAIIVTPRSDIHVSAAVTCSKSLNFLLKIRSGGHDYEGLSYI 128

Query: 127 SDVVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGV 186
           SD  PFF+LDM NLR+++VD   ++AW+  GATLGEVYYR+ EK+K +GFPAGVCPTVGV
Sbjct: 129 SD-KPFFILDMSNLRDVSVDIADQSAWISAGATLGEVYYRIWEKSKVHGFPAGVCPTVGV 188

Query: 187 GGHFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVV 246
           GGH  GGGYGNM+RK GLSVD +IDAKI+DVNG++LDRK+MGEDLFWAI GGGG+SFGVV
Sbjct: 189 GGHISGGGYGNMLRKFGLSVDNLIDAKIVDVNGQILDRKSMGEDLFWAISGGGGASFGVV 248

Query: 247 VAYKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDK 306
           + YK+++V VPE VTVFRV++ ++   + ++V +WQ V    D +LF+R+    V  T K
Sbjct: 249 LGYKVKLVPVPETVTVFRVEKYMDSGAV-DMVHKWQSVGPKTDRNLFLRMLIQPV--TRK 308

Query: 307 EKKTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPPGTSVE 366
           + KT+RAT +ALFLG +  ++ ++   FP+L LKK +C EM+W QS L+W N    T ++
Sbjct: 309 KVKTVRATVVALFLGRAEEVVALLGKEFPELSLKKENCSEMTWFQSALWWDNRVNPTQID 368

Query: 367 --ALLSRTPQVLTHLKRKSDYVKAPIPKEGLNKIWKKMIELEKPMMTFNPYGGRMAEIPS 426
               L R        KRKSDYV + IP++G+  ++KKM EL K  + FNPYGG+MAE+  
Sbjct: 369 PKVFLDRNLDRANFGKRKSDYVASEIPRDGIESLFKKMTELGKIGLVFNPYGGKMAEVTV 428

Query: 427 NATPFPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRD 486
           NATPFPHR+  L+KIQY+  W E      K F++    LY FMT FVSKNPR A+LNYRD
Sbjct: 429 NATPFPHRS-KLFKIQYSVTWQENSVEIEKGFLNQANVLYSFMTGFVSKNPRNAYLNYRD 488

Query: 487 LDIGVNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIP 528
           +DIGVN +G NSY EG VYG KYF  NF+RLVK+KT  DP NFFRNEQSIP
Sbjct: 489 VDIGVNDHGTNSYEEGEVYGRKYFGDNFDRLVKVKTAADPDNFFRNEQSIP 533

BLAST of Cmc12g0327701 vs. TAIR 10
Match: AT1G11770.1 (FAD-binding Berberine family protein )

HSP 1 Score: 563.1 bits (1450), Expect = 2.3e-160
Identity = 289/530 (54.53%), Postives = 372/530 (70.19%), Query Frame = 0

Query: 8   ILFLSFSFLVFFSSASSSAPE----NFLQCLLRHS-PPSYPIAPAIFTPNNASYSSVLET 67
           ILFL  SF +  S A    PE    NFLQC    +  P   +A  +     A+++ VL  
Sbjct: 7   ILFLISSF-ISTSLAVEPPPETIYQNFLQCFTNQTKAPPNSLADVVLPKTAAAFTPVLRA 66

Query: 68  YIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSDVV 127
           YIRN RFNT+ TPKP +++ A  ESHV+ A++C +  N+Q+K RSGGHDYEGVSY+S  V
Sbjct: 67  YIRNARFNTTATPKPAIVIAARSESHVQAAVICTKSLNIQLKTRSGGHDYEGVSYISH-V 126

Query: 128 PFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGGHF 187
           PFFVLDM NLR ITVD  TE+AWV  GATLGEVYYR+ EK KS+GFPAGVCPTVG GGH 
Sbjct: 127 PFFVLDMSNLRNITVDPATESAWVGAGATLGEVYYRIWEKTKSHGFPAGVCPTVGAGGHI 186

Query: 188 GGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYK 247
            GGGYGNM+RK+GLSVD + DAKI+DVNG++LDRK MGED+FWAI GGGG+SFGV++A+K
Sbjct: 187 SGGGYGNMIRKYGLSVDYVTDAKIVDVNGQVLDRKGMGEDMFWAINGGGGASFGVILAFK 246

Query: 248 IRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEKKT 307
           I++V VP  VTVFRV++ L +N  TE+V +WQ VA   D  LF+R+    V  T  + +T
Sbjct: 247 IKLVPVPPTVTVFRVEKNLVEN-ATEMVHKWQFVAPKTDPGLFMRLLLQPV--TRNKMQT 306

Query: 308 LRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPPGTSV--EAL 367
           +RA+ +ALFLGD   ++ ++   FP+LGLKK +C EM+W+QSV++W N    T +  E L
Sbjct: 307 VRASVVALFLGDQNTVMSMLTKEFPELGLKKENCTEMTWIQSVMWWANNDNATQIKPEIL 366

Query: 368 LSRTPQVLTHLKRKSDYVKAPIPKEGLNKIWKKMIELEKPMMTFNPYGGRMAEIPSNATP 427
           L R P + T  KRKSD+V+  I K+GL+ ++KKMIE+ K  + FNPYGG M+ + +  TP
Sbjct: 367 LDRNPDMATFGKRKSDFVEKEITKDGLDFLFKKMIEVGKIGLVFNPYGGIMSTVATTKTP 426

Query: 428 FPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 487
           FPHR   L+KIQ++ NW + G      F+   +  Y +M PFV+KNPR  ++NYRDLDIG
Sbjct: 427 FPHRK-KLYKIQHSMNWKDPGTEAETSFLQKAKSFYSYMAPFVTKNPRHTYINYRDLDIG 486

Query: 488 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFP 531
           VN  G NSY    V+G  YF  NF+RLVK+KT VDP NFFR+EQSIP  P
Sbjct: 487 VNTPGPNSYRVAEVFGRMYFGENFDRLVKVKTAVDPQNFFRDEQSIPTLP 530

BLAST of Cmc12g0327701 vs. TAIR 10
Match: AT4G20830.1 (FAD-binding Berberine family protein )

HSP 1 Score: 561.6 bits (1446), Expect = 6.7e-160
Identity = 286/523 (54.68%), Postives = 373/523 (71.32%), Query Frame = 0

Query: 8   ILFLSFSFLVFFSSASSSAPENFLQCLL-RHSPPSYPIAPAIFTPNNASYSSVLETYIRN 67
           +LFLS     F  S S+S   +FL+C   +   P   I   +F+  N ++SSVL  YIRN
Sbjct: 19  LLFLSLPLSSF--SQSNSVYNSFLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLRAYIRN 78

Query: 68  LRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSDVVPFFV 127
            RFNTS+T KP +I+T   ESHV  A+ C++  N  +KIRSGGHDY+G+SY+SD  PFF+
Sbjct: 79  ARFNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISD-KPFFI 138

Query: 128 LDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGGHFGGGG 187
           LDM N+R+++VD  + +AW+  GATLGEVYYR+ EK++ +GFPAGVCPTVGVGGH  GGG
Sbjct: 139 LDMSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHLSGGG 198

Query: 188 YGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIRMV 247
           YGNM+RK GLSVD + DAKI+DVNG++LDRKAMGEDLFWAI GGGG S+GVV+ YK+++V
Sbjct: 199 YGNMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYKVKLV 258

Query: 248 RVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEKKTLRAT 307
            VP  VTVFRV++ ++   + ++V +WQ V    D +LF+R+    V  T K+ KT+RA+
Sbjct: 259 PVPSVVTVFRVEQYMDSGAV-DMVHKWQSVGPKTDPNLFMRMLIQPV--TRKKVKTVRAS 318

Query: 308 FIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPPGTSVE--ALLSRT 367
            +ALFLG +  ++ +++  FP+LGLKK +C EM+W QS L+W N    T V+    L R 
Sbjct: 319 VVALFLGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDRN 378

Query: 368 PQVLTHLKRKSDYVKAPIPKEGLNKIWKKMIELEKPMMTFNPYGGRMAEIPSNATPFPHR 427
               +  KRKSDYV   IPK+G+  ++KKMIEL K  + FNPYGG+MAE+  NA PFPHR
Sbjct: 379 LDTSSFGKRKSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPHR 438

Query: 428 AGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIGVNHN 487
              L+KIQY+ NW E      K +++  + LY FMT FVSKNPR+++ NYRD+DIGVN +
Sbjct: 439 -NKLFKIQYSVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVNDH 498

Query: 488 GKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIP 528
           G NSY EG VYG KYF  NF+RLVKIKT VDP NFFRNEQSIP
Sbjct: 499 GANSYKEGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIP 534

BLAST of Cmc12g0327701 vs. TAIR 10
Match: AT4G20830.2 (FAD-binding Berberine family protein )

HSP 1 Score: 561.6 bits (1446), Expect = 6.7e-160
Identity = 286/523 (54.68%), Postives = 373/523 (71.32%), Query Frame = 0

Query: 8   ILFLSFSFLVFFSSASSSAPENFLQCLL-RHSPPSYPIAPAIFTPNNASYSSVLETYIRN 67
           +LFLS     F  S S+S   +FL+C   +   P   I   +F+  N ++SSVL  YIRN
Sbjct: 19  LLFLSLPLSSF--SQSNSVYNSFLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLRAYIRN 78

Query: 68  LRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSDVVPFFV 127
            RFNTS+T KP +I+T   ESHV  A+ C++  N  +KIRSGGHDY+G+SY+SD  PFF+
Sbjct: 79  ARFNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISD-KPFFI 138

Query: 128 LDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGGHFGGGG 187
           LDM N+R+++VD  + +AW+  GATLGEVYYR+ EK++ +GFPAGVCPTVGVGGH  GGG
Sbjct: 139 LDMSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHLSGGG 198

Query: 188 YGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIRMV 247
           YGNM+RK GLSVD + DAKI+DVNG++LDRKAMGEDLFWAI GGGG S+GVV+ YK+++V
Sbjct: 199 YGNMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYKVKLV 258

Query: 248 RVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEKKTLRAT 307
            VP  VTVFRV++ ++   + ++V +WQ V    D +LF+R+    V  T K+ KT+RA+
Sbjct: 259 PVPSVVTVFRVEQYMDSGAV-DMVHKWQSVGPKTDPNLFMRMLIQPV--TRKKVKTVRAS 318

Query: 308 FIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPPGTSVE--ALLSRT 367
            +ALFLG +  ++ +++  FP+LGLKK +C EM+W QS L+W N    T V+    L R 
Sbjct: 319 VVALFLGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDRN 378

Query: 368 PQVLTHLKRKSDYVKAPIPKEGLNKIWKKMIELEKPMMTFNPYGGRMAEIPSNATPFPHR 427
               +  KRKSDYV   IPK+G+  ++KKMIEL K  + FNPYGG+MAE+  NA PFPHR
Sbjct: 379 LDTSSFGKRKSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPHR 438

Query: 428 AGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIGVNHN 487
              L+KIQY+ NW E      K +++  + LY FMT FVSKNPR+++ NYRD+DIGVN +
Sbjct: 439 -NKLFKIQYSVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVNDH 498

Query: 488 GKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIP 528
           G NSY EG VYG KYF  NF+RLVKIKT VDP NFFRNEQSIP
Sbjct: 499 GANSYKEGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIP 534

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008445642.12.9e-30999.62PREDICTED: reticuline oxidase-like protein [Cucumis melo] >KAA0047124.1 reticuli... [more]
XP_004135723.12.2e-29895.86berberine bridge enzyme-like 8 [Cucumis sativus] >KAE8653304.1 hypothetical prot... [more]
XP_038895093.11.0e-29092.86berberine bridge enzyme-like 8 [Benincasa hispida][more]
XP_022994779.15.9e-27588.72berberine bridge enzyme-like 8 [Cucurbita maxima][more]
KAG6573419.11.3e-27488.72Berberine bridge enzyme-like 8, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q9SA851.6e-19564.64Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2... [more]
Q9SVG33.5e-16155.56Berberine bridge enzyme-like 21 OS=Arabidopsis thaliana OX=3702 GN=At4g20840 PE=... [more]
Q9SA993.2e-15954.53Berberine bridge enzyme-like 2 OS=Arabidopsis thaliana OX=3702 GN=At1g11770 PE=2... [more]
Q9SVG49.4e-15954.68Berberine bridge enzyme-like 19 OS=Arabidopsis thaliana OX=3702 GN=At4g20830 PE=... [more]
F4HV091.0e-15753.33Berberine bridge enzyme-like 14 OS=Arabidopsis thaliana OX=3702 GN=At1g34575 PE=... [more]
Match NameE-valueIdentityDescription
A0A5A7TVZ21.4e-30999.62Reticuline oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A1S3BE211.4e-30999.62reticuline oxidase-like protein OS=Cucumis melo OX=3656 GN=LOC103488597 PE=3 SV=... [more]
A0A6J1K3U32.8e-27588.72berberine bridge enzyme-like 8 OS=Cucurbita maxima OX=3661 GN=LOC111490407 PE=3 ... [more]
A0A6J1GSQ17.0e-27488.35berberine bridge enzyme-like 8 OS=Cucurbita moschata OX=3662 GN=LOC111456785 PE=... [more]
A0A6J1DGF36.2e-26284.38berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111020214 PE... [more]
Match NameE-valueIdentityDescription
AT1G30700.11.2e-19664.64FAD-binding Berberine family protein [more]
AT4G20840.12.5e-16255.56FAD-binding Berberine family protein [more]
AT1G11770.12.3e-16054.53FAD-binding Berberine family protein [more]
AT4G20830.16.7e-16054.68FAD-binding Berberine family protein [more]
AT4G20830.26.7e-16054.68FAD-binding Berberine family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.462.20coord: 251..466
e-value: 2.0E-164
score: 549.5
NoneNo IPR availablePANTHERPTHR32448OS08G0158400 PROTEINcoord: 18..528
NoneNo IPR availablePANTHERPTHR32448:SF25BERBERINE BRIDGE ENZYME-LIKE 8coord: 18..528
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 136..527
e-value: 2.0E-164
score: 549.5
IPR012951Berberine/berberine-likePFAMPF08031BBEcoord: 470..527
e-value: 4.5E-21
score: 74.7
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 80..214
e-value: 4.0E-23
score: 81.7
IPR016167FAD-binding, type PCMH, subdomain 1GENE3D3.30.43.10coord: 25..132
e-value: 3.0E-40
score: 138.3
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 73..248
score: 14.658849
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 20..249

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc12g0327701.1Cmc12g0327701.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0071949 FAD binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0050660 flavin adenine dinucleotide binding