Cmc12g0316471 (gene) Melon (Charmono) v1.1

Overview


Name	Cmc12g0316471
Type	gene
Organism	Cucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Description	NAD(P)-binding Rossmann-fold superfamily protein
Location	CMiso1.1chr12: 1508662 .. 1512713 (-)
RNA-Seq Expression	Cmc12g0316471
Synteny	Cmc12g0316471

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTATGATTGTCACTGAACTATTCTCCATTTTGACTTGCTGTGATTGTATAAACTGAACTGAGTGTTTTTTCTTTCTGAGTACTCCGAAAGTTAGAAAAAGCTTGTATAATTTAGCATAGCAAATCGCATGGTGGCAAAACCTCCAATCTGTTAAGGCCACCCCCCAAAGCAAGAGGGCAGAAGAATGTTACTGAACTATTCTCCATTTTGACTTGCTGTGATCGTATAAACTGAACTGAGTGTTTTTTCTTTCTGAATACTCCGAAAGTTAGAAAAAGCTTGTATAATTTAGCATAGCAAATCGCATGGTGGCAAAACCTCCAATCTGTTAAGGCCACCCCCCAAAGCAAGAGGGCAGAAGAATGTTACTGGCTCTTGTTTCTCCTTCATCCTTTCCTTCATTCTCTTCTTCTACCTTCAACAAATCCCATTTCTCAACATTCTCTTCATTTCCCATTTTGTGCTCATCCAACCCTTTTTGCCTTTCCCCATCACATTCCACTCACAAACTCACCAAACGCCTGAACCCCATTAGAAATCAAACTGAATCAGACTTCAAAAGCAAATATGGTCATACTCCAGTTGACGATCGGTGGTCTCTTCGCGGAAAGACAGCCCTCGTTACCGGTGGCACTCGTGGAATTGGGTAAATCAATATCTGTATGAACAAATAGAGTCGATTTTCACTCTTTTTTTTGTTGCATTTACTGTTTCTTCGTCTGTTTTATTGAGAATTTGGAATTGGGTTTTGGTCTAGGCGTGCGATTGTGGAGGAATTGGTGGGTTTTGGGGCGAGAGTGCATACTTGTTCTCGGAATGAAGGTGAGTTGAGACAGTGTTTGAGGCATTGGAAGGATTTGGAATTTGAGGTTTCTGGGTCAGTCTGTGATGTGTCTGTTCGAGCTCAGAGAGAGGAGTTAATGGAGAATGCGGGTAATACTTTTGATGGGAAGCTCAACATTCTTGTAAGGTGCACCCTTTCTTGCGTTTTTCTCTGCTTTAGATAAAGATGCTATTTTTCTTTGTTGGGCTACTTTTCTTTCAATCACTCACTTACTGAGCTTAAATTGATTAAATTATTGGAAGGAACCTTCTAGCTGCCACTCTCATTAGAAATCTAACAAGAACATCAATTTTCTTCTCTATTGTGCTCGCAATTGTTATAAAGGTGACAGCATTGAATGCTGTTCTCGATAAAAATTATTTGGCTAAGAGTAAGACCTTTTTGCAACTCTATGAATCATTGCAATGAACTGTTGAGGAAGCTATATGAACTACTTTTTCTTTTGATACTCGTGAGTGTTCGGGATAGTATTGGACAATTCAACTGGAACAATCTGCAACCCTACAACATTTAGATATTAAGGAAATTCCTAGGATATTAAATCTCAAGTAAATGATCAACATTGATTGAACTCATCCTCTTTTAGTCCTTTATCAACTCAATTTACTAAGAACATGTCGCCGTGGTATAGGATGAAGAAAGCTTTTAATTGACATGGGATTAACGTAGTTTCCACATATAGGAGGACTTGAAGACGAGCAATTAAAGAAGGCCAAATTCAAGAGACAACAAGCCTTGGAAGCCCACACATCTTGCATATTTTGAACTATGCATTGGGATTTACTTTGAGGTGATAGACCTCCAATAATATTTCAATATAGTAGAAGAAATAAGAAGAGGAATTAGTGGGAGTGGCAGTTTTGTGATAAAAGACTGTTAATAGAGAGTTTGTTAGAAATTTGTTATGTTAGAGAGAGGTCGGTGCTAGGGAGTATAAAAGAGACGTTTAGGCGGGAAAAAGGGATATGTGTAGTTTGAGAAAGAAAGTAGGCTTGTACTTCTTGAGAGGAGAAGACAAGAGAGTTTCCTCATTGAATTGAGTTCTACTCATTCAATCTACATTGTAATTGATTTCATACTATCAATAAAGTCCAGCACTATTCAATTAGTTGGAGTCTACCATGAGGCTTTAGACTTTTAGCTAGACTCCTTGTTAGGATTCTTAATGTGTTTTTAAGTACCTTTACGTTAGTCACTCACCCTCAAAGGCGAAGGACGAACATTTTAGGTTTTAGCTGTTAAGCATCTTGTCACTTTTATTATTTTTCATTATTTCTTTTAGTAGTTGTGCAAGCTAATTTTAAGCATTTGTTTTTGAGAGTTTATCATGTCCCGTTGTCGTTCACTTAAGGCTTTGTAAGGCCTGATTCATACTTGTAAAATCGTTTGATTTCTTGATGATGAAATGAGTTTGAAAATTCTCTCTTTTGCTCATTGTTGAGAGCATGTGCGTACGGGCAATTTTATACCTAGTTTTCAAATTTCACTTTCGTTTAAAAACATAGTTCAACTTCTTGGCCAATTTGTGGAGTGTTTCACACTTGAGAATAGGAACTTGTTATCCTTGCTTCCGGGTATTGGACCTCTAAAGGCAACCCCATCCTAACATTCCTTTGAGCGCGCGCTTGTGAAGCATTTGTGATTCAAGGTCCACGCCTAAGGGTTTTATACCAATTTATCACGGTCTCGCTTGTTGTTTGGTATCAAAGCTTTTGATTTGTATCAACAATTTTAAATGGGTAATGCCAATGTCGTCAAAGGCTAAACCCTTTTTGGGATATTTTCTCAAGGAGAAATGAGGGTTAAGTCTTGAGGCAGCATGACCCGTAAGCTTCTTATATTAGACACATAAATCTGTTCTAGTCCAAAGAAGGGTAAGTAAGATTAAAAGGTTAAGAATTTTAGTATCAGCCTTGAGGTGAGCGAGCTTAAGGTGTAAGCCTCAAAATCCTTGTAAAACATTAGATGAGGATGAGACAATAGAGTGGTACCTCTATTCAGTTGATGCTGATCATTTAGCGAAAAAAATTTTGGTTTACTATTTCTTAAATAAATTAATTTCGCCTTAAAGATTCTTCAAATAAACCCTTTGGTGGATCTGCACTTTTTAAATGTGTATGTAACTTGTAAGTTATTTCCTACTGAAATTTAATCCTTAGGATATTTGCTGCTTTTTGGCATATGGAATTTTTACTTCTCTTTTTATAATGTGAAGTTTACTAAGGCTGAGGTTGATTTTAAATTAGCTACGAACTCATCTTCCAACATCATATGGGCAAACCCAAAATTTAAGAATAAAAAAGCACATCGATTTCATCATTTTTTTAATCTTTATGGACCACGAACACCATTTCTGCTACCATGTAGGTGAATAATGTGGGAAGAAATATACGAAAGCCAACAACAGAATTCACAGATGAAGAATTTTCTTTTCTTATGAAAACCAATGTTGAATCAGTCTTTCATTTATCACAACTTGCTTACCCTCTTCTAAAATCATCTGGCGAGGGAAGCATCGTATTCATGTCCTCAGTCTCAAGTTTTGTGTCTCTGAAGTCCATGTCCGTTCAGGGAGCGACCAAAGGTGATCAACTTCATCCATCGTATTTAAATATTATAGATGCAAACATCAATTTCTTTCTTGGAATATTTCCTATCTATTTGTGATGATTCTTTTCAGGAGCTATCAATCAACTCACAAAATATTTAGCTTGTGAATGGGCAAAAGACAACATAAGGAGCAATGCAGTTGCCCCGTGGTACATCAAAACGTCAATGGTGGAACAGGTAAACCTCGAATGCCGCCATTATGTTTTAACCTGTGAAGAATCGATCATAATGTTCATCTTCTTGATCTTTCGCTATACGTTTTCTTTTAGGTGCTGAGTAACAAAACATACGTAGAAGAGGTATATTCCCGAACCCCGCTTCGACGGTTGGGAGAACCAAGTGAAGTATCATCCTTAGTTGCATTCCTTTGCCTACCTGCTTCATCTTATATTACTGGCCAAATCATTGGTGTTGATGGAGGGATGTCTGTTAATGGCTTTGACCCAAAATAACATTTGAATGAACATCTTCTGTTTGTCCCTAATCCCCTCTCAGATATGTCTAGTTTGAGTGTCGATTTTTCTTTCATTGCTATTCTGATACCTGTTTTTTTTTTTTTTTTTTTCTCTCTTTCCTTTTTAAATACAACAGTCTGACT

mRNA sequence

GTATGATTGTCACTGAACTATTCTCCATTTTGACTTGCTGTGATTGTATAAACTGAACTGAGTGTTTTTTCTTTCTGAGTACTCCGAAAGTTAGAAAAAGCTTGTATAATTTAGCATAGCAAATCGCATGGTGGCAAAACCTCCAATCTGTTAAGGCCACCCCCCAAAGCAAGAGGGCAGAAGAATGTTACTGAACTATTCTCCATTTTGACTTGCTGTGATCGTATAAACTGAACTGAGTGTTTTTTCTTTCTGAATACTCCGAAAGTTAGAAAAAGCTTGTATAATTTAGCATAGCAAATCGCATGGTGGCAAAACCTCCAATCTGTTAAGGCCACCCCCCAAAGCAAGAGGGCAGAAGAATGTTACTGGCTCTTGTTTCTCCTTCATCCTTTCCTTCATTCTCTTCTTCTACCTTCAACAAATCCCATTTCTCAACATTCTCTTCATTTCCCATTTTGTGCTCATCCAACCCTTTTTGCCTTTCCCCATCACATTCCACTCACAAACTCACCAAACGCCTGAACCCCATTAGAAATCAAACTGAATCAGACTTCAAAAGCAAATATGGTCATACTCCAGTTGACGATCGGTGGTCTCTTCGCGGAAAGACAGCCCTCGTTACCGGTGGCACTCGTGGAATTGGGCGTGCGATTGTGGAGGAATTGGTGGGTTTTGGGGCGAGAGTGCATACTTGTTCTCGGAATGAAGGTGAGTTGAGACAGTGTTTGAGGCATTGGAAGGATTTGGAATTTGAGGTTTCTGGGTCAGTCTGTGATGTGTCTGTTCGAGCTCAGAGAGAGGAGTTAATGGAGAATGCGGGTAATACTTTTGATGGGAAGCTCAACATTCTTGTGAATAATGTGGGAAGAAATATACGAAAGCCAACAACAGAATTCACAGATGAAGAATTTTCTTTTCTTATGAAAACCAATGTTGAATCAGTCTTTCATTTATCACAACTTGCTTACCCTCTTCTAAAATCATCTGGCGAGGGAAGCATCGTATTCATGTCCTCAGTCTCAAGTTTTGTGTCTCTGAAGTCCATGTCCGTTCAGGGAGCGACCAAAGGAGCTATCAATCAACTCACAAAATATTTAGCTTGTGAATGGGCAAAAGACAACATAAGGAGCAATGCAGTTGCCCCGTGGTACATCAAAACGTCAATGGTGGAACAGGTGCTGAGTAACAAAACATACGTAGAAGAGGTATATTCCCGAACCCCGCTTCGACGGTTGGGAGAACCAAGTGAAGTATCATCCTTAGTTGCATTCCTTTGCCTACCTGCTTCATCTTATATTACTGGCCAAATCATTGGTGTTGATGGAGGGATGTCTGTTAATGGCTTTGACCCAAAATAACATTTGAATGAACATCTTCTGTTTGTCCCTAATCCCCTCTCAGATATGTCTAGTTTGAGTGTCGATTTTTCTTTCATTGCTATTCTGATACCTGTTTTTTTTTTTTTTTTTTTCTCTCTTTCCTTTTTAAATACAACAGTCTGACT

Coding sequence (CDS)

ATGTTACTGGCTCTTGTTTCTCCTTCATCCTTTCCTTCATTCTCTTCTTCTACCTTCAACAAATCCCATTTCTCAACATTCTCTTCATTTCCCATTTTGTGCTCATCCAACCCTTTTTGCCTTTCCCCATCACATTCCACTCACAAACTCACCAAACGCCTGAACCCCATTAGAAATCAAACTGAATCAGACTTCAAAAGCAAATATGGTCATACTCCAGTTGACGATCGGTGGTCTCTTCGCGGAAAGACAGCCCTCGTTACCGGTGGCACTCGTGGAATTGGGCGTGCGATTGTGGAGGAATTGGTGGGTTTTGGGGCGAGAGTGCATACTTGTTCTCGGAATGAAGGTGAGTTGAGACAGTGTTTGAGGCATTGGAAGGATTTGGAATTTGAGGTTTCTGGGTCAGTCTGTGATGTGTCTGTTCGAGCTCAGAGAGAGGAGTTAATGGAGAATGCGGGTAATACTTTTGATGGGAAGCTCAACATTCTTGTGAATAATGTGGGAAGAAATATACGAAAGCCAACAACAGAATTCACAGATGAAGAATTTTCTTTTCTTATGAAAACCAATGTTGAATCAGTCTTTCATTTATCACAACTTGCTTACCCTCTTCTAAAATCATCTGGCGAGGGAAGCATCGTATTCATGTCCTCAGTCTCAAGTTTTGTGTCTCTGAAGTCCATGTCCGTTCAGGGAGCGACCAAAGGAGCTATCAATCAACTCACAAAATATTTAGCTTGTGAATGGGCAAAAGACAACATAAGGAGCAATGCAGTTGCCCCGTGGTACATCAAAACGTCAATGGTGGAACAGGTGCTGAGTAACAAAACATACGTAGAAGAGGTATATTCCCGAACCCCGCTTCGACGGTTGGGAGAACCAAGTGAAGTATCATCCTTAGTTGCATTCCTTTGCCTACCTGCTTCATCTTATATTACTGGCCAAATCATTGGTGTTGATGGAGGGATGTCTGTTAATGGCTTTGACCCAAAATAA

Protein sequence

MLLALVSPSSFPSFSSSTFNKSHFSTFSSFPILCSSNPFCLSPSHSTHKLTKRLNPIRNQTESDFKSKYGHTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELRQCLRHWKDLEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSVNGFDPK

Homology

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BLAST of Cmc12g0316471 vs. NCBI nr
Match: XP_008457446.1 (PREDICTED: tropinone reductase homolog At5g06060 [Cucumis melo] >TYJ97378.1 tropinone reductase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 655.6 bits (1690), Expect = 2.3e-184
Identity = 332/332 (100.00%), Postives = 332/332 (100.00%), Query Frame = 0


Query: 1   MLLALVSPSSFPSFSSSTFNKSHFSTFSSFPILCSSNPFCLSPSHSTHKLTKRLNPIRNQ 60
           MLLALVSPSSFPSFSSSTFNKSHFSTFSSFPILCSSNPFCLSPSHSTHKLTKRLNPIRNQ
Sbjct: 1   MLLALVSPSSFPSFSSSTFNKSHFSTFSSFPILCSSNPFCLSPSHSTHKLTKRLNPIRNQ 60

Query: 61  TESDFKSKYGHTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELR 120
           TESDFKSKYGHTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELR
Sbjct: 61  TESDFKSKYGHTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELR 120

Query: 121 QCLRHWKDLEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFT 180
           QCLRHWKDLEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFT
Sbjct: 121 QCLRHWKDLEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFT 180

Query: 181 DEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGAIN 240
           DEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGAIN
Sbjct: 181 DEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGAIN 240

Query: 241 QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSEVSS 300
           QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSEVSS
Sbjct: 241 QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSEVSS 300

Query: 301 LVAFLCLPASSYITGQIIGVDGGMSVNGFDPK 333
           LVAFLCLPASSYITGQIIGVDGGMSVNGFDPK
Sbjct: 301 LVAFLCLPASSYITGQIIGVDGGMSVNGFDPK 332

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BLAST of Cmc12g0316471 vs. NCBI nr
Match: XP_031741141.1 (tropinone reductase homolog At5g06060 [Cucumis sativus])

HSP 1 Score: 630.9 bits (1626), Expect = 6.0e-177
Identity = 322/332 (96.99%), Postives = 322/332 (96.99%), Query Frame = 0


Query: 1   MLLALVSPSSFPSFSSSTFNKSHFSTFSSFPILCSSNPFCLSPSHSTHKLTKRLNPIRNQ 60
           MLL LVSPSSFPSFSSSTFNKS FSTFSSFPILCS N FCL PSHSTHKL KRLNPIRNQ
Sbjct: 1   MLLVLVSPSSFPSFSSSTFNKSPFSTFSSFPILCSFNSFCLFPSHSTHKLIKRLNPIRNQ 60

Query: 61  TESDFKSKYGHTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELR 120
           TESDF SKYG TPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELR
Sbjct: 61  TESDFNSKYGRTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELR 120

Query: 121 QCLRHWKDLEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFT 180
           QCLRHWKDLEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFT
Sbjct: 121 QCLRHWKDLEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFT 180

Query: 181 DEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGAIN 240
           DEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGAIN
Sbjct: 181 DEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGAIN 240

Query: 241 QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSEVSS 300
           QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNK Y EEVYSRTPLRRLGEPSEVSS
Sbjct: 241 QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYEEEVYSRTPLRRLGEPSEVSS 300

Query: 301 LVAFLCLPASSYITGQIIGVDGGMSVNGFDPK 333
           LVAFLCLPASSYITGQIIGVDGGMSVNGFDPK
Sbjct: 301 LVAFLCLPASSYITGQIIGVDGGMSVNGFDPK 332

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BLAST of Cmc12g0316471 vs. NCBI nr
Match: XP_038893799.1 (tropinone reductase homolog At5g06060-like [Benincasa hispida])

HSP 1 Score: 586.3 bits (1510), Expect = 1.7e-163
Identity = 304/335 (90.75%), Postives = 313/335 (93.43%), Query Frame = 0


Query: 1   MLLALVSPSSFPSFSSSTFNK---SHFSTFSSFPILCSSNPFCLSPSHSTHKLTKRLNPI 60
           MLLALVSPSSF SFSSSTFN+      STFSSFPI CS  PF LSPSHS+HKL KRL PI
Sbjct: 1   MLLALVSPSSFYSFSSSTFNRPTNPPLSTFSSFPISCSFKPFWLSPSHSSHKLVKRLKPI 60

Query: 61  RNQTESDFKSKYGHTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEG 120
            NQTESDFK KYG TPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEG
Sbjct: 61  TNQTESDFKIKYGSTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEG 120

Query: 121 ELRQCLRHWKDLEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTT 180
           ELRQCLR W +L+FEVSGSVCDVSVRAQR+ELME   +TFDGKL+ILVNNVGRNIRKPTT
Sbjct: 121 ELRQCLRDWNNLKFEVSGSVCDVSVRAQRDELMEIVADTFDGKLSILVNNVGRNIRKPTT 180

Query: 181 EFTDEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKG 240
           EFTDEEFSFLMKTNVE+VFHLSQLAYPLLKSSGEGSIVFMSSVSS VSLKSMSVQGATKG
Sbjct: 181 EFTDEEFSFLMKTNVEAVFHLSQLAYPLLKSSGEGSIVFMSSVSSLVSLKSMSVQGATKG 240

Query: 241 AINQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSE 300
           AINQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSE
Sbjct: 241 AINQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSE 300

Query: 301 VSSLVAFLCLPASSYITGQIIGVDGGMSVNGFDPK 333
           VSSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP+
Sbjct: 301 VSSLVAFLCLPASSYITGQIIGVDGGMSVNGFDPR 335

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BLAST of Cmc12g0316471 vs. NCBI nr
Match: XP_022998606.1 (tropinone reductase homolog At5g06060-like [Cucurbita maxima])

HSP 1 Score: 554.7 bits (1428), Expect = 5.5e-154
Identity = 291/333 (87.39%), Postives = 300/333 (90.09%), Query Frame = 0


Query: 1   MLLALVSPSSFPSFSSSTFNKS--HFSTFSSFPILCSSNPFCLSPSHSTHKLTKRLNPIR 60
           M LALVSPS F S SSSTFN S  H STFSS PI  S  PF L  S+STHKL KRL  IR
Sbjct: 1   MFLALVSPSPFDSLSSSTFNPSQPHRSTFSSSPISRSLKPFWLPTSNSTHKLVKRLKSIR 60

Query: 61  NQTESDFKSKYGHTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGE 120
           NQ ESDFK+K G TP D RWSL G TALVTGGTRGIGRAIVEELVGFGARVHTCSRNE E
Sbjct: 61  NQAESDFKAKNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNESE 120

Query: 121 LRQCLRHWKDLEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTE 180
           L+QCLR WKDLEFEVSGSVCDVSVRAQREELM+   +TF+GKLNILVNNVGRNIRKPTTE
Sbjct: 121 LKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTFNGKLNILVNNVGRNIRKPTTE 180

Query: 181 FTDEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGA 240
           FTDEEFSFLMKTN+ESVFHLSQLA+P LK+SGEGSIVFMSSVSSFVSLKSMSVQGATKGA
Sbjct: 181 FTDEEFSFLMKTNIESVFHLSQLAHPFLKASGEGSIVFMSSVSSFVSLKSMSVQGATKGA 240

Query: 241 INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSEV 300
           INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNK YVEEVYSRTPLRRLGEPSEV
Sbjct: 241 INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEV 300

Query: 301 SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP 332
           SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP
Sbjct: 301 SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP 333

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BLAST of Cmc12g0316471 vs. NCBI nr
Match: KAG6607269.1 (Tropinone reductase-like, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 553.1 bits (1424), Expect = 1.6e-153
Identity = 291/333 (87.39%), Postives = 298/333 (89.49%), Query Frame = 0


Query: 1   MLLALVSPSSFPSFSSSTFNKS--HFSTFSSFPILCSSNPFCLSPSHSTHKLTKRLNPIR 60
           M LALVSPS   SFSSSTFN S  H STFSS PI  S  PF L  S+STHKL KRL  IR
Sbjct: 1   MFLALVSPSPSDSFSSSTFNPSQPHLSTFSSSPISRSFKPFWLPTSNSTHKLVKRLKSIR 60

Query: 61  NQTESDFKSKYGHTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGE 120
           NQ ESDFK+K G TP D RWSL G TALVTGGTRGIGRAIVEELVGFGARVHTCSRNE E
Sbjct: 61  NQAESDFKTKNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENE 120

Query: 121 LRQCLRHWKDLEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTE 180
           L+QCLR WKDLEFEVSGSVCDVSVRAQREELM+   +TFDGKLNILVNNVGRNIRKPTTE
Sbjct: 121 LKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTFDGKLNILVNNVGRNIRKPTTE 180

Query: 181 FTDEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGA 240
           FTDEEFSFLMKTNVESVFHLSQLA+P LK+SG GSIVFMSSVSSFVSLKSMSVQGATKGA
Sbjct: 181 FTDEEFSFLMKTNVESVFHLSQLAHPFLKASGAGSIVFMSSVSSFVSLKSMSVQGATKGA 240

Query: 241 INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSEV 300
           INQLTKYLACEWA DNIRSNAVAPWYIKTSMVEQVLSNK YVEEVYSRTPLRRLGEPSEV
Sbjct: 241 INQLTKYLACEWANDNIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEV 300

Query: 301 SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP 332
           SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP
Sbjct: 301 SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP 333

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BLAST of Cmc12g0316471 vs. ExPASy Swiss-Prot
Match: Q9ZW12 (Tropinone reductase homolog At2g29260, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g29260 PE=2 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 7.6e-106
Identity = 201/286 (70.28%), Postives = 221/286 (77.27%), Query Frame = 0


Query: 52  KRLNPIRNQTESDFKSKYGH-----TPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFG 111
           KR+NP    + S     Y           +RWSL G +ALVTGGTRGIGRAIVEEL G G
Sbjct: 35  KRINPKHKSSSSSVFVPYASQSSIAITSKERWSLNGMSALVTGGTRGIGRAIVEELAGLG 94

Query: 112 ARVHTCSRNEGELRQCLRHWKDLEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVN 171
           A VHTC+RNE EL  CL  W    F V+GSVCDVS R+QRE LME   + F+GKL+ILVN
Sbjct: 95  AEVHTCARNEYELENCLSDWNRSGFRVAGSVCDVSDRSQREALMETVSSVFEGKLHILVN 154

Query: 172 NVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSL 231
           NVG NIRKP  EFT  EFS LM TN ESVFHL QLAYPLL+ S  GS+VF+SSVS FVSL
Sbjct: 155 NVGTNIRKPMVEFTAGEFSTLMSTNFESVFHLCQLAYPLLRESKAGSVVFISSVSGFVSL 214

Query: 232 KSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSR 291
           K+MSVQ +TKGAINQLT+ LACEWAKDNIR NAVAPWYIKTSMVEQVLSNK Y+EEVYS 
Sbjct: 215 KNMSVQSSTKGAINQLTRSLACEWAKDNIRINAVAPWYIKTSMVEQVLSNKEYLEEVYSV 274

Query: 292 TPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSVNGFDPK 333
           TPL RLGEP EVSS VAFLCLPASSYITGQI+ VDGGMS+NGF P+
Sbjct: 275 TPLGRLGEPREVSSAVAFLCLPASSYITGQILCVDGGMSINGFFPR 320

[Close]

BLAST of Cmc12g0316471 vs. ExPASy Swiss-Prot
Match: Q9LHT0 (Tropinone reductase homolog At5g06060 OS=Arabidopsis thaliana OX=3702 GN=At5g06060 PE=2 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 1.1e-99
Identity = 179/255 (70.20%), Postives = 209/255 (81.96%), Query Frame = 0


Query: 75  DDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELRQCLRHWKDLEFEVS 134
           D RWSL GKTALVTGGTRGIGRA+VEEL  FGA+VHTCSRN+ EL  CL  WK     VS
Sbjct: 4   DKRWSLAGKTALVTGGTRGIGRAVVEELAKFGAKVHTCSRNQEELNACLNDWKANGLVVS 63

Query: 135 GSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFTDEEFSFLMKTNVES 194
           GSVCD SVR QRE+L++ A + F GKLNIL+NNVG N+RKPT E++ EE++ +M TN+ES
Sbjct: 64  GSVCDASVRDQREKLIQEASSAFSGKLNILINNVGTNVRKPTVEYSSEEYAKIMSTNLES 123

Query: 195 VFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDN 254
            FHLSQ+A+PLLK+SG GSIVF+SSV+  V L S S+ GATKGA+NQLT+ LACEWA DN
Sbjct: 124 AFHLSQIAHPLLKASGVGSIVFISSVAGLVHLSSGSIYGATKGALNQLTRNLACEWASDN 183

Query: 255 IRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYIT 314
           IR+N VAPWYIKTS+VE +L  K +VE V SRTPL R+GEP EVSSLVAFLCLPASSYIT
Sbjct: 184 IRTNCVAPWYIKTSLVETLLEKKEFVEAVVSRTPLGRVGEPEEVSSLVAFLCLPASSYIT 243

Query: 315 GQIIGVDGGMSVNGF 330
           GQ+I VDGG +VNGF
Sbjct: 244 GQVISVDGGFTVNGF 258

[Close]

BLAST of Cmc12g0316471 vs. ExPASy Swiss-Prot
Match: Q9ZW13 (Tropinone reductase homolog At2g29290 OS=Arabidopsis thaliana OX=3702 GN=At2g29290 PE=2 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 3.5e-87
Identity = 158/256 (61.72%), Postives = 202/256 (78.91%), Query Frame = 0


Query: 74  VDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELRQCLRHWKDLEFEV 133
           +D RWSL+G  ALVTGGT+GIG A+VEEL   GARVHTC+R+E +L++ LR W++  F+V
Sbjct: 1   MDKRWSLQGMNALVTGGTKGIGEAVVEELSILGARVHTCARDETQLQERLREWQEKGFQV 60

Query: 134 SGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFTDEEFSFLMKTNVE 193
           + S+CDVS+R QRE+LME   + F GKLNILVNNVG  + KPTTE+T EEFSFLM TN++
Sbjct: 61  TTSICDVSLREQREKLMETVSSLFQGKLNILVNNVGTLMLKPTTEYTAEEFSFLMATNLD 120

Query: 194 SVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKD 253
           S FH+SQLA+PLLK+SG GSIV MSS++  V +   S+ GATKGA+NQL + LACEWA D
Sbjct: 121 SAFHISQLAHPLLKASGSGSIVLMSSIAGVVHVGVGSIYGATKGAMNQLARNLACEWASD 180

Query: 254 NIRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYI 313
           NIR+NA+ PW I T ++  +LS +   +E   RTP+ R+GE +EVS LVAFLCLPA+SYI
Sbjct: 181 NIRTNAICPWLITTPLISDLLSVEEMKKEAEERTPMGRVGEANEVSPLVAFLCLPAASYI 240

Query: 314 TGQIIGVDGGMSVNGF 330
           TGQ+I VDGG++VNGF
Sbjct: 241 TGQVICVDGGLTVNGF 256

[Close]

BLAST of Cmc12g0316471 vs. ExPASy Swiss-Prot
Match: Q9ZW19 (Tropinone reductase homolog At2g29360 OS=Arabidopsis thaliana OX=3702 GN=SDR PE=1 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 2.5e-85
Identity = 159/256 (62.11%), Postives = 199/256 (77.73%), Query Frame = 0


Query: 77  RWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELRQCLRHWKDLEFEVSGS 136
           RWSL G TALVTGG++GIG A+VEEL   GAR+HTC+R+E +L++ LR W+   F+V+ S
Sbjct: 13  RWSLVGMTALVTGGSKGIGEAVVEELATLGARIHTCARDETQLQESLRKWQAKGFQVTTS 72

Query: 137 VCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVF 196
           VCDVS R +RE+LME     F+GKLNILVNNVG  I KPT + T E+FSF M TN+ES F
Sbjct: 73  VCDVSSRDKREKLMETVSTIFEGKLNILVNNVGTCIVKPTLQHTAEDFSFTMATNLESAF 132

Query: 197 HLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIR 256
           HLSQLA+PLLK+SG GSIV +SSVS  V +   S+ G +KGA+NQL + LACEWA DNIR
Sbjct: 133 HLSQLAHPLLKASGSGSIVLISSVSGVVHVNGASIYGVSKGAMNQLGRNLACEWASDNIR 192

Query: 257 SNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQ 316
           +N+V PW+I+T +V + LSN+ + +EV SR P+ R+GE +EVSSLVAFLCLPA+SYITGQ
Sbjct: 193 TNSVCPWFIETPLVTESLSNEEFRKEVESRPPMGRVGEVNEVSSLVAFLCLPAASYITGQ 252

Query: 317 IIGVDGGMSVNGFDPK 333
            I VDGG +VNGF  K
Sbjct: 253 TICVDGGFTVNGFSFK 268

[Close]

BLAST of Cmc12g0316471 vs. ExPASy Swiss-Prot
Match: A0A1C9II22 (Noroxomaritidine/norcraugsodine reductase OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222 GN=NR PE=1 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 7.3e-85
Identity = 151/255 (59.22%), Postives = 199/255 (78.04%), Query Frame = 0


Query: 74  VDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELRQCLRHWKDLEFEV 133
           ++ RWSL G TALVTGGT+GIG AIVEELVGFGARV+TCSRNE ELR+CL+ W++L+++V
Sbjct: 3   LEKRWSLEGTTALVTGGTKGIGHAIVEELVGFGARVYTCSRNEAELRKCLQEWENLKYDV 62

Query: 134 SGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFTDEEFSFLMKTNVE 193
           +GSVCDVS R +RE+L E   + F+GKLNIL+NN G  + KP   FT E+FSFL+  N+E
Sbjct: 63  TGSVCDVSSRTEREKLAEEVSSVFNGKLNILINNAGGYVNKPIDGFTAEDFSFLVAVNLE 122

Query: 194 SVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKD 253
           S FHL QLA+P+LK+SG GSIV +SS  + +++   S+  +TKGAINQLT+ LACEWAKD
Sbjct: 123 SAFHLCQLAHPMLKASGTGSIVHISSCCAQIAIPGHSIYSSTKGAINQLTRNLACEWAKD 182

Query: 254 NIRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYI 313
           NIR+N++AP  I+T   E  + +K  ++   SR P  R+GEP EV+SL AFLC+P++SYI
Sbjct: 183 NIRTNSIAPGAIRTPGTESFVIDKDALDREVSRVPFGRIGEPEEVASLAAFLCMPSASYI 242

Query: 314 TGQIIGVDGGMSVNG 329
           TGQ+I VDGG ++NG
Sbjct: 243 TGQVICVDGGRTING 257

[Close]

BLAST of Cmc12g0316471 vs. ExPASy TrEMBL
Match: A0A5D3BGS1 (Tropinone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G001920 PE=4 SV=1)

HSP 1 Score: 655.6 bits (1690), Expect = 1.1e-184
Identity = 332/332 (100.00%), Postives = 332/332 (100.00%), Query Frame = 0


Query: 1   MLLALVSPSSFPSFSSSTFNKSHFSTFSSFPILCSSNPFCLSPSHSTHKLTKRLNPIRNQ 60
           MLLALVSPSSFPSFSSSTFNKSHFSTFSSFPILCSSNPFCLSPSHSTHKLTKRLNPIRNQ
Sbjct: 1   MLLALVSPSSFPSFSSSTFNKSHFSTFSSFPILCSSNPFCLSPSHSTHKLTKRLNPIRNQ 60

Query: 61  TESDFKSKYGHTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELR 120
           TESDFKSKYGHTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELR
Sbjct: 61  TESDFKSKYGHTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELR 120

Query: 121 QCLRHWKDLEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFT 180
           QCLRHWKDLEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFT
Sbjct: 121 QCLRHWKDLEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFT 180

Query: 181 DEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGAIN 240
           DEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGAIN
Sbjct: 181 DEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGAIN 240

Query: 241 QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSEVSS 300
           QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSEVSS
Sbjct: 241 QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSEVSS 300

Query: 301 LVAFLCLPASSYITGQIIGVDGGMSVNGFDPK 333
           LVAFLCLPASSYITGQIIGVDGGMSVNGFDPK
Sbjct: 301 LVAFLCLPASSYITGQIIGVDGGMSVNGFDPK 332

[Close]

BLAST of Cmc12g0316471 vs. ExPASy TrEMBL
Match: A0A1S3C5I5 (tropinone reductase homolog At5g06060 OS=Cucumis melo OX=3656 GN=LOC103497133 PE=4 SV=1)

HSP 1 Score: 655.6 bits (1690), Expect = 1.1e-184
Identity = 332/332 (100.00%), Postives = 332/332 (100.00%), Query Frame = 0


Query: 1   MLLALVSPSSFPSFSSSTFNKSHFSTFSSFPILCSSNPFCLSPSHSTHKLTKRLNPIRNQ 60
           MLLALVSPSSFPSFSSSTFNKSHFSTFSSFPILCSSNPFCLSPSHSTHKLTKRLNPIRNQ
Sbjct: 1   MLLALVSPSSFPSFSSSTFNKSHFSTFSSFPILCSSNPFCLSPSHSTHKLTKRLNPIRNQ 60

Query: 61  TESDFKSKYGHTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELR 120
           TESDFKSKYGHTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELR
Sbjct: 61  TESDFKSKYGHTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELR 120

Query: 121 QCLRHWKDLEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFT 180
           QCLRHWKDLEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFT
Sbjct: 121 QCLRHWKDLEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFT 180

Query: 181 DEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGAIN 240
           DEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGAIN
Sbjct: 181 DEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGAIN 240

Query: 241 QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSEVSS 300
           QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSEVSS
Sbjct: 241 QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSEVSS 300

Query: 301 LVAFLCLPASSYITGQIIGVDGGMSVNGFDPK 333
           LVAFLCLPASSYITGQIIGVDGGMSVNGFDPK
Sbjct: 301 LVAFLCLPASSYITGQIIGVDGGMSVNGFDPK 332

[Close]

BLAST of Cmc12g0316471 vs. ExPASy TrEMBL
Match: A0A6J1KAL5 (tropinone reductase homolog At5g06060-like OS=Cucurbita maxima OX=3661 GN=LOC111493193 PE=4 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 2.7e-154
Identity = 291/333 (87.39%), Postives = 300/333 (90.09%), Query Frame = 0


Query: 1   MLLALVSPSSFPSFSSSTFNKS--HFSTFSSFPILCSSNPFCLSPSHSTHKLTKRLNPIR 60
           M LALVSPS F S SSSTFN S  H STFSS PI  S  PF L  S+STHKL KRL  IR
Sbjct: 1   MFLALVSPSPFDSLSSSTFNPSQPHRSTFSSSPISRSLKPFWLPTSNSTHKLVKRLKSIR 60

Query: 61  NQTESDFKSKYGHTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGE 120
           NQ ESDFK+K G TP D RWSL G TALVTGGTRGIGRAIVEELVGFGARVHTCSRNE E
Sbjct: 61  NQAESDFKAKNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNESE 120

Query: 121 LRQCLRHWKDLEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTE 180
           L+QCLR WKDLEFEVSGSVCDVSVRAQREELM+   +TF+GKLNILVNNVGRNIRKPTTE
Sbjct: 121 LKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTFNGKLNILVNNVGRNIRKPTTE 180

Query: 181 FTDEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGA 240
           FTDEEFSFLMKTN+ESVFHLSQLA+P LK+SGEGSIVFMSSVSSFVSLKSMSVQGATKGA
Sbjct: 181 FTDEEFSFLMKTNIESVFHLSQLAHPFLKASGEGSIVFMSSVSSFVSLKSMSVQGATKGA 240

Query: 241 INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSEV 300
           INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNK YVEEVYSRTPLRRLGEPSEV
Sbjct: 241 INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEV 300

Query: 301 SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP 332
           SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP
Sbjct: 301 SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP 333

[Close]

BLAST of Cmc12g0316471 vs. ExPASy TrEMBL
Match: A0A6J1GAE7 (tropinone reductase homolog At5g06060-like OS=Cucurbita moschata OX=3662 GN=LOC111452252 PE=4 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 8.5e-153
Identity = 289/333 (86.79%), Postives = 297/333 (89.19%), Query Frame = 0


Query: 1   MLLALVSPSSFPSFSSSTFNKS--HFSTFSSFPILCSSNPFCLSPSHSTHKLTKRLNPIR 60
           M LALVSPS F S SSSTF+ S  H STFSS PI  S  PF L  S+STHKL KRL  IR
Sbjct: 1   MFLALVSPSPFDSLSSSTFSPSQPHLSTFSSSPISRSFKPFWLPTSNSTHKLVKRLKSIR 60

Query: 61  NQTESDFKSKYGHTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGE 120
           NQ  SDFK+K G TP D RWSL G TALVTGGTRGIGRAIVEELVGFGARVHTCSRNE E
Sbjct: 61  NQAASDFKTKNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENE 120

Query: 121 LRQCLRHWKDLEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTE 180
           L+QCLR WKDLEFEVSGSVCDVSVRAQREELM+   +TFDGKLNILVNNVGRNIRKPTTE
Sbjct: 121 LKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTFDGKLNILVNNVGRNIRKPTTE 180

Query: 181 FTDEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGA 240
           FTDEEFSFLMKTNVESVFHLSQLA+P LK+SG GSIVFMSSVSSFVSLKSMSVQGATKGA
Sbjct: 181 FTDEEFSFLMKTNVESVFHLSQLAHPFLKASGAGSIVFMSSVSSFVSLKSMSVQGATKGA 240

Query: 241 INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSEV 300
           INQLTKYLACEWA DNIRSNAVAPWYIKTSMVEQVLSNK YVEEVYSRTPLRRLGEPSEV
Sbjct: 241 INQLTKYLACEWANDNIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEV 300

Query: 301 SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP 332
           SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP
Sbjct: 301 SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP 333

[Close]

BLAST of Cmc12g0316471 vs. ExPASy TrEMBL
Match: A0A6J1CL23 (tropinone reductase homolog At5g06060-like OS=Momordica charantia OX=3673 GN=LOC111012129 PE=4 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 8.8e-150
Identity = 281/332 (84.64%), Postives = 292/332 (87.95%), Query Frame = 0


Query: 1   MLLALVSPSSFPSFSSSTFNKSHFSTFSSFPILCSSNPFCLSPSHSTHKLTKRLNPIRNQ 60
           MLLALVSP+SF SFSS TF        S   I CS NPF L PS+ST K+  RL PI NQ
Sbjct: 1   MLLALVSPTSFDSFSSFTFITPTNPPLSPISISCSFNPFGLPPSYSTRKILHRLKPITNQ 60

Query: 61  TESDFKSKYGHTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELR 120
           T+ D KS  G TP+DDRWSLRG TALVTGGTRGIGRAIVEELVGFGARVHTCSRN  EL+
Sbjct: 61  TQPDLKSMNGTTPIDDRWSLRGATALVTGGTRGIGRAIVEELVGFGARVHTCSRNLSELQ 120

Query: 121 QCLRHWKDLEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFT 180
           QCLR WK+LEF VSGSVCDVSVRAQREELME     FDGKLNILVNNVGRNIRKPT EFT
Sbjct: 121 QCLRDWKNLEFGVSGSVCDVSVRAQREELMETVAANFDGKLNILVNNVGRNIRKPTAEFT 180

Query: 181 DEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGAIN 240
           +EEFSFLMKTNVESVFHLSQLAYPLLK+SG GSIVFMSSVSSFVSLKSMSVQGATKGAIN
Sbjct: 181 EEEFSFLMKTNVESVFHLSQLAYPLLKASGRGSIVFMSSVSSFVSLKSMSVQGATKGAIN 240

Query: 241 QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSEVSS 300
           QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNK YVEEVYSRTPLRRLGEPSEVSS
Sbjct: 241 QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKVYVEEVYSRTPLRRLGEPSEVSS 300

Query: 301 LVAFLCLPASSYITGQIIGVDGGMSVNGFDPK 333
           LVAFLCLPASSYITGQIIGVDGGMS+NGFDP+
Sbjct: 301 LVAFLCLPASSYITGQIIGVDGGMSINGFDPR 332

[Close]

BLAST of Cmc12g0316471 vs. TAIR 10
Match: AT2G29260.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 385.2 bits (988), Expect = 5.4e-107
Identity = 201/286 (70.28%), Postives = 221/286 (77.27%), Query Frame = 0


Query: 52  KRLNPIRNQTESDFKSKYGH-----TPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFG 111
           KR+NP    + S     Y           +RWSL G +ALVTGGTRGIGRAIVEEL G G
Sbjct: 35  KRINPKHKSSSSSVFVPYASQSSIAITSKERWSLNGMSALVTGGTRGIGRAIVEELAGLG 94

Query: 112 ARVHTCSRNEGELRQCLRHWKDLEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVN 171
           A VHTC+RNE EL  CL  W    F V+GSVCDVS R+QRE LME   + F+GKL+ILVN
Sbjct: 95  AEVHTCARNEYELENCLSDWNRSGFRVAGSVCDVSDRSQREALMETVSSVFEGKLHILVN 154

Query: 172 NVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSL 231
           NVG NIRKP  EFT  EFS LM TN ESVFHL QLAYPLL+ S  GS+VF+SSVS FVSL
Sbjct: 155 NVGTNIRKPMVEFTAGEFSTLMSTNFESVFHLCQLAYPLLRESKAGSVVFISSVSGFVSL 214

Query: 232 KSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSR 291
           K+MSVQ +TKGAINQLT+ LACEWAKDNIR NAVAPWYIKTSMVEQVLSNK Y+EEVYS 
Sbjct: 215 KNMSVQSSTKGAINQLTRSLACEWAKDNIRINAVAPWYIKTSMVEQVLSNKEYLEEVYSV 274

Query: 292 TPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSVNGFDPK 333
           TPL RLGEP EVSS VAFLCLPASSYITGQI+ VDGGMS+NGF P+
Sbjct: 275 TPLGRLGEPREVSSAVAFLCLPASSYITGQILCVDGGMSINGFFPR 320

[Close]

BLAST of Cmc12g0316471 vs. TAIR 10
Match: AT5G06060.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 364.8 bits (935), Expect = 7.5e-101
Identity = 179/255 (70.20%), Postives = 209/255 (81.96%), Query Frame = 0


Query: 75  DDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELRQCLRHWKDLEFEVS 134
           D RWSL GKTALVTGGTRGIGRA+VEEL  FGA+VHTCSRN+ EL  CL  WK     VS
Sbjct: 4   DKRWSLAGKTALVTGGTRGIGRAVVEELAKFGAKVHTCSRNQEELNACLNDWKANGLVVS 63

Query: 135 GSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFTDEEFSFLMKTNVES 194
           GSVCD SVR QRE+L++ A + F GKLNIL+NNVG N+RKPT E++ EE++ +M TN+ES
Sbjct: 64  GSVCDASVRDQREKLIQEASSAFSGKLNILINNVGTNVRKPTVEYSSEEYAKIMSTNLES 123

Query: 195 VFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDN 254
            FHLSQ+A+PLLK+SG GSIVF+SSV+  V L S S+ GATKGA+NQLT+ LACEWA DN
Sbjct: 124 AFHLSQIAHPLLKASGVGSIVFISSVAGLVHLSSGSIYGATKGALNQLTRNLACEWASDN 183

Query: 255 IRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYIT 314
           IR+N VAPWYIKTS+VE +L  K +VE V SRTPL R+GEP EVSSLVAFLCLPASSYIT
Sbjct: 184 IRTNCVAPWYIKTSLVETLLEKKEFVEAVVSRTPLGRVGEPEEVSSLVAFLCLPASSYIT 243

Query: 315 GQIIGVDGGMSVNGF 330
           GQ+I VDGG +VNGF
Sbjct: 244 GQVISVDGGFTVNGF 258

[Close]

BLAST of Cmc12g0316471 vs. TAIR 10
Match: AT2G29290.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 323.2 bits (827), Expect = 2.5e-88
Identity = 158/256 (61.72%), Postives = 202/256 (78.91%), Query Frame = 0


Query: 74  VDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELRQCLRHWKDLEFEV 133
           +D RWSL+G  ALVTGGT+GIG A+VEEL   GARVHTC+R+E +L++ LR W++  F+V
Sbjct: 1   MDKRWSLQGMNALVTGGTKGIGEAVVEELSILGARVHTCARDETQLQERLREWQEKGFQV 60

Query: 134 SGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFTDEEFSFLMKTNVE 193
           + S+CDVS+R QRE+LME   + F GKLNILVNNVG  + KPTTE+T EEFSFLM TN++
Sbjct: 61  TTSICDVSLREQREKLMETVSSLFQGKLNILVNNVGTLMLKPTTEYTAEEFSFLMATNLD 120

Query: 194 SVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKD 253
           S FH+SQLA+PLLK+SG GSIV MSS++  V +   S+ GATKGA+NQL + LACEWA D
Sbjct: 121 SAFHISQLAHPLLKASGSGSIVLMSSIAGVVHVGVGSIYGATKGAMNQLARNLACEWASD 180

Query: 254 NIRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYI 313
           NIR+NA+ PW I T ++  +LS +   +E   RTP+ R+GE +EVS LVAFLCLPA+SYI
Sbjct: 181 NIRTNAICPWLITTPLISDLLSVEEMKKEAEERTPMGRVGEANEVSPLVAFLCLPAASYI 240

Query: 314 TGQIIGVDGGMSVNGF 330
           TGQ+I VDGG++VNGF
Sbjct: 241 TGQVICVDGGLTVNGF 256

[Close]

BLAST of Cmc12g0316471 vs. TAIR 10
Match: AT2G29360.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 317.0 bits (811), Expect = 1.8e-86
Identity = 159/256 (62.11%), Postives = 199/256 (77.73%), Query Frame = 0


Query: 77  RWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELRQCLRHWKDLEFEVSGS 136
           RWSL G TALVTGG++GIG A+VEEL   GAR+HTC+R+E +L++ LR W+   F+V+ S
Sbjct: 13  RWSLVGMTALVTGGSKGIGEAVVEELATLGARIHTCARDETQLQESLRKWQAKGFQVTTS 72

Query: 137 VCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVF 196
           VCDVS R +RE+LME     F+GKLNILVNNVG  I KPT + T E+FSF M TN+ES F
Sbjct: 73  VCDVSSRDKREKLMETVSTIFEGKLNILVNNVGTCIVKPTLQHTAEDFSFTMATNLESAF 132

Query: 197 HLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIR 256
           HLSQLA+PLLK+SG GSIV +SSVS  V +   S+ G +KGA+NQL + LACEWA DNIR
Sbjct: 133 HLSQLAHPLLKASGSGSIVLISSVSGVVHVNGASIYGVSKGAMNQLGRNLACEWASDNIR 192

Query: 257 SNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQ 316
           +N+V PW+I+T +V + LSN+ + +EV SR P+ R+GE +EVSSLVAFLCLPA+SYITGQ
Sbjct: 193 TNSVCPWFIETPLVTESLSNEEFRKEVESRPPMGRVGEVNEVSSLVAFLCLPAASYITGQ 252

Query: 317 IIGVDGGMSVNGFDPK 333
            I VDGG +VNGF  K
Sbjct: 253 TICVDGGFTVNGFSFK 268

[Close]

BLAST of Cmc12g0316471 vs. TAIR 10
Match: AT2G29370.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 313.9 bits (803), Expect = 1.5e-85
Identity = 156/253 (61.66%), Postives = 197/253 (77.87%), Query Frame = 0


Query: 77  RWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELRQCLRHWKDLEFEVSGS 136
           +WSL G TALVTGG++G+G+A+VEEL   GARVHTC+R+E +L++ LR W+    +V+ S
Sbjct: 13  KWSLEGMTALVTGGSKGLGKAVVEELAMLGARVHTCARDETQLQESLREWQAKGLQVTTS 72

Query: 137 VCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVF 196
           VCDVS R QRE+LME   + F GKL+ILV NVG  + KPTTE T EEFSF++ TN+ES F
Sbjct: 73  VCDVSSRDQREKLMETVSSLFQGKLSILVPNVGIGVLKPTTECTAEEFSFIIATNLESTF 132

Query: 197 HLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIR 256
           H SQLA+PLLK+SG G+IV MSSV+  V+L + S+ GATKGA+NQL + LACEWA DNIR
Sbjct: 133 HFSQLAHPLLKASGSGNIVLMSSVAGVVNLGNTSIYGATKGAMNQLARNLACEWASDNIR 192

Query: 257 SNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQ 316
           +N+V PW+I T   +  L +K   E+V S TPLRR+GE +EVSSLVAFLCLPA+SYITGQ
Sbjct: 193 ANSVCPWFITTPSTKDFLGDKDVKEKVESVTPLRRVGEANEVSSLVAFLCLPAASYITGQ 252

Query: 317 IIGVDGGMSVNGF 330
            I VDGG ++NGF
Sbjct: 253 TICVDGGFTINGF 265

The following BLAST results are available for this feature:

BLAST of Cmc12g0316471 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008457446.1	2.3e-184	100.00	PREDICTED: tropinone reductase homolog At5g06060 [Cucumis melo] >TYJ97378.1 trop...	[more]
XP_031741141.1	6.0e-177	96.99	tropinone reductase homolog At5g06060 [Cucumis sativus]	[more]
XP_038893799.1	1.7e-163	90.75	tropinone reductase homolog At5g06060-like [Benincasa hispida]	[more]
XP_022998606.1	5.5e-154	87.39	tropinone reductase homolog At5g06060-like [Cucurbita maxima]	[more]
KAG6607269.1	1.6e-153	87.39	Tropinone reductase-like, chloroplastic, partial [Cucurbita argyrosperma subsp. ...	[more]

BLAST of Cmc12g0316471 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZW12	7.6e-106	70.28	Tropinone reductase homolog At2g29260, chloroplastic OS=Arabidopsis thaliana OX=...	[more]
Q9LHT0	1.1e-99	70.20	Tropinone reductase homolog At5g06060 OS=Arabidopsis thaliana OX=3702 GN=At5g060...	[more]
Q9ZW13	3.5e-87	61.72	Tropinone reductase homolog At2g29290 OS=Arabidopsis thaliana OX=3702 GN=At2g292...	[more]
Q9ZW19	2.5e-85	62.11	Tropinone reductase homolog At2g29360 OS=Arabidopsis thaliana OX=3702 GN=SDR PE=...	[more]
A0A1C9II22	7.3e-85	59.22	Noroxomaritidine/norcraugsodine reductase OS=Narcissus aff. pseudonarcissus MK-2...	[more]

BLAST of Cmc12g0316471 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BGS1	1.1e-184	100.00	Tropinone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3C5I5	1.1e-184	100.00	tropinone reductase homolog At5g06060 OS=Cucumis melo OX=3656 GN=LOC103497133 PE...	[more]
A0A6J1KAL5	2.7e-154	87.39	tropinone reductase homolog At5g06060-like OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A6J1GAE7	8.5e-153	86.79	tropinone reductase homolog At5g06060-like OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1CL23	8.8e-150	84.64	tropinone reductase homolog At5g06060-like OS=Momordica charantia OX=3673 GN=LOC...	[more]

BLAST of Cmc12g0316471 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G29260.1	5.4e-107	70.28	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G06060.1	7.5e-101	70.20	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G29290.2	2.5e-88	61.72	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G29360.1	1.8e-86	62.11	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G29370.1	1.5e-85	61.66	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 232..251 score: 36.93 coord: 253..270 score: 39.59 coord: 288..308 score: 34.57 coord: 84..101 score: 51.89 coord: 159..170 score: 50.8 coord: 206..222 score: 30.43
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00080	SDRFAMILY	coord: 232..251 score: 31.56 coord: 212..220 score: 48.0 coord: 159..170 score: 43.41
None	No IPR available	PFAM	PF13561	adh_short_C2	coord: 91..325 e-value: 6.7E-59 score: 199.3
None	No IPR available	GENE3D	3.40.50.720		coord: 73..328 e-value: 3.4E-80 score: 270.9
None	No IPR available	PIRSR	PIRSR000094-3	PIRSR000094-3	coord: 79..329 e-value: 2.0E-22 score: 77.5
None	No IPR available	PANTHER	PTHR42898:SF2	TROPINONE REDUCTASE-LIKE PROTEIN, CHLOROPLASTIC	coord: 57..332
IPR045000	Tropinone reductase	PANTHER	PTHR42898	TROPINONE REDUCTASE	coord: 57..332
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 81..326

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cmc12g0316471.1	Cmc12g0316471.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity