Cmc10g0277441 (gene) Melon (Charmono) v1.1

Overview


Name	Cmc10g0277441
Type	gene
Organism	Cucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Description	tRNA (guanine(26)-N(2))-dimethyltransferase
Location	CMiso1.1chr10: 13267713 .. 13272013 (+)
RNA-Seq Expression	Cmc10g0277441
Synteny	Cmc10g0277441

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTAACATAATTTATTAAAAAAAAAAAAACAAAACACAAAAACCTATAAAATCCATGAAAATGCAAAATCCAACGATTTCCCGCCATGTTAACTGTAGCGTCAACAACCCTATCTCCTTCCCCGTTTCTTTACATCCAACAAACAAACTCCCATTTCCTCACCTCTCCATCCATTCATCTCTTCCAATTTTCACACTCCAACAAAAAATCCCCCAACAATTGTCTCTCAAACAATCGCACCGAAAGGGGCTTAGATTTCGACATCGGAGACACCTTCTTCCGCCATGAAAGCGCCACCGGACGCGACTTGGGTGTCCTCGCCGCCGCGCTCTACCGAAAATCCAAGGGCCATCTTCGGATTCTCGACGCATTGTGCGGTTGTGGGATCCGGTCGCTTCGGTACTTGGTCGAGTCCGAGGCTGATTTTGTGTTGGCGAATGACGCTAATGATGAAAATCGGAGGGTTATTGTGAAGAATCTCTCGAAAGTTGCCGAAAATTTTGGGGATAATCGACGGTGGTTGGTTACTAATTGTGATGCGAACAGGGTAATGACGGAGTTTTACTTGAAGAAGGATTTCTTTGATTTTATTGATGTTGATTCCTTTGGAAGTGATTCGTCGTTTCTGAGACCCGCTATAAATGCTTTGAAATTGGATGGATTGCTCTATGTTACGTCCACGGATGGTTTCTCTTCCGGCGGACATCGGCCTTCTCAGTGAGTGCATAATCTTACTCTTTAGTTATCTGCTTTAACCGGTTCATTAAAATCTTGATATGAAATGTCTTATCATTTTAATGTCCTGTACCTATATATAAAGGTGCTGAAGGATTGATAATGGTTATAATTAAATTTAACAATAACTCAATGTTTAAGCTCTTGGGTCAATGAGTGATTAGATATGGTATAGAGTTGATATGTTTAAGCTGTATCATGTTCTAATGTCCATCTTTTCCCCACTTAATATTGATTTTCACTTTTTTTTTTGAGAATTTCATATAAATTTTCAAGTCGACAAGTGTGGTGGAATATTATTCTTATAATTAAATTTATCATAAATCATCAACTTAAATTCTTGGGTCAATCATTTAATAAAAGGATTTTAAATGATTAAGTATAGTTATTTTAGTTTTGAGTTTTAGTAGTTGAATTTGGTGTTAGCTCCCCATTAATGCTGTTAGTTACTTGTTGAGTCTTTGTCTCTAGGACTAAGCAAATCGACTTGTAACTGATAGATTACTTAAAACTGACTTTTTAGCTGAATGTTTTAAGAAATTGAACTGAACTAGCTAAATTGACAAAACCAGCTGAAATTGAATAAGGGTTGCTATTTGCTAAAAATAACAAACTTTATCCAAATCATTTTGATTGTTTTTGGTTTTGACTTCCATCCTTACCTGTACTAAGTTTTAAGCCTAAAAGTGAAATGGTTGCTAAGTATATGAATATAGGTGGATAATTATATATGAAGATGTGGTAATTAAAATTCTTTTCTCTCCTTTTTCTTGTCTCTCTTTTCTTCTTTCCATTTCTCTCTAGATCTTTAGCTGCATATGGAGCATATATACGTCCCATGCCATTTTCAAATGAAATTGGTCTGCGGATGCTTATAGGTGGGGTTGCACGTGAAGCTTCTGTACTGGGTTACTATGCTACTCCACTCTTTTCGTATTATTCGTACCATGGACCTGTTTTCAGAGTCATGCTACGAATCAATCGAGGGAGGATTTCTAAAAACAGGTGATAGTTGATGAGTTTAGCTTTGTCTGCTAGTGTTGGATGTATCTAGTATGATCATTGATGGTGATTGATCGAAGCTCTCACTATTCAATATCAGGCATTATGGTTTTGTCAGCTATTGCGAAAAATGTGGAAATTCACAAGCTTTTTCATGGAGTGAGCTTGGACAGATGAGCTGTCCTTGCAATGATTCAAGGGTAAGATTATGACTACTATGACGATGCTTGAAATGATTCATGAATATGCTTGGATTTGCTGTTTTCTTATTCATTTGGGTTCTTGCTTTCTAGTGTATTTACTTGCAACATTCAATCTTGTAAGAGTCTCGAGATACATTAAAATTTTTTGACTTGTTAGGTTTCTGAGTCACTAGTGGTCTCAGGACCTCTTTGGATTGGACCTCTACACAGTGCAGACTACATTGAAGACATATTAACCTTGGCCAAGCAATGGGAATGGATAGGCAATGGCCAGGGGAAGGATCTTGAGAAACTTCTGATGCAGATGCTGGATGAGAGTGACTCCAAACTACCAGTAGGGTACATCAAAATGGACGAGGTGGCCAAATTTTATTTATTCTTCTCGTGATTTAATATTTTATCTTTCTTTTTGATCCAATAATGCAAGAAGTGATCTATCAAGGCCCCCCTTCTCTATGCTTAGGTGGCAAGTCGGGCAAAAGTAAACTCACCTCCCCTCGGTACCATGATGAGCGAGATGATCAAGGTGAGAATACTGGTAGTTAAGATGTGATAAATTGTGTGATGCATATTATTCCACATGGCTCAACTGTTTTTGTTGTTGTGATATTCTCTTGAATGCTTGAAAGTAGAGAAATGTTGTTGTATATATTAAACCACCAATTCACCCAAGAGCTTAAGCTGGTGATTGAAGACAAATTTAATTATATATCACTAACAGTATACATGAGACACAAGGCACAAATACGATAGGACACAAAAACAATGATGGGTTATTCTCTATGAGTAGGAAATGGCCATGGCGTGGATATGATTACTAAAATTTATATGTTTTTAAGCTATATCGTTTTTATTCAATTTGAAGTCTGTAAGCTTTGTGTGTATATATATCTATTTGAAAAATTAAGTTTGGATATGGTTTGCTTCAATATTTATTATTATTATTTTGCATTATCCCTATACATATCTAAGTATTAATCTTAACAAATATTTGACACTAGTCGAACAAGTGCAAAAACTTGTATTCAATTTGTTCAACTACTGTCCAACAGTTGTATACTTGTCTTGTGGGTACAAGGATATCACACGTGTTCATCATGTTTCGGAGTGTCCAATAAGCGTCGGACTTGGACGATTTTTCCAAACTATAATGTTTTTTTTTTTTTCTTTTGCTTGGAATGCAGATGCTTGAAAAGAAAACAATTCTCTCTTTTCTCTCTTCTCTCTTTCTGAGGGTTTTGGTTGTGGCAGGCTGGATATGCTGCTAGTAGGTCTCATATTGCCTCAAACGCAATCAAGACTAACTGTCCTATGGCAGACTGCATCAGAATTGCTCAACTGCAGCAAAATTGTCTTGGTGTGCATCAAGTCACATGAATGTTGTAGCCATAGCTTGGGGCTATGGAAACTACAACCCTTCATACGCAAACAAGAACGAAATGAAACCAATGTAAGCATCCATCTCTCTTTAGTTATCTCATTTTGAAAATTGATGAAGATAATTATGGGTCAAATGCGTTAAAAGTGGTAAAGAACCATGTCCGATGCTCTCAAGAAATTTGCGTTGCAGGTTTTGGTGACGAGAGACAACCAAGTTAGATCGAAAAACTTTAACTTTCTGTTGCATTCTGTCAAAGATAGACAAGAAATGAGCACATACAGACACAAGACAGGGTCACAGAGACACCATGCCATTTCATTTAAAGTGTCGTATTGATGCTTTATAGAAGTAGATAACTAAAATGTTTATATCAAATTCATGCATATTAAGTGTAAATTAATTAGTTTATTATATGAGCCAGGATGAGATCACTGAAGCAGTTGAACTTCGATTTTGAAGAACGAAAGCTGTGCGGAGCTCAAGTACGGGACTTGATAAAGAACCTTCCCAGACTTGTTAGCCACAACTCTTTATTTATACTTCTTATAGAGCCATGATGTGATGTGATGTGATGTTCTCATCTTAGGCGCAAAATGAAATATTAAGCTAGAAAGTGCAGGCCAATAGTAAAATCTACCTTCTCAGTTTTTAGTTGAACATTGCTGACAAGACTAACCTAATATGTAGGGATATATTATGAAATTTTCAATTTTTGTGTGCAACAGGAAAAGTAACTCAATCATAAATGGCATAGTCTACCTCCTTGTAGGTTCAAGATTATATCCCTATTCCTGTGATTGTCGTACCAAAGCAAATTGTGTGTATAATAGAATATTGTTGCAAAATTCACATGCACCTAAAGCACGCTGACTAAATTTGTAAGGATTATGGGAAAAGCTGGTATCGAATGGGTTTAATGGATTTTTGCAAGTTTGTTTGCCTCGTTCCTTCACTATGCCAAACACTAAAATTCACCTAAGA

mRNA sequence

TTAACATAATTTATTAAAAAAAAAAAAACAAAACACAAAAACCTATAAAATCCATGAAAATGCAAAATCCAACGATTTCCCGCCATGTTAACTGTAGCGTCAACAACCCTATCTCCTTCCCCGTTTCTTTACATCCAACAAACAAACTCCCATTTCCTCACCTCTCCATCCATTCATCTCTTCCAATTTTCACACTCCAACAAAAAATCCCCCAACAATTGTCTCTCAAACAATCGCACCGAAAGGGGCTTAGATTTCGACATCGGAGACACCTTCTTCCGCCATGAAAGCGCCACCGGACGCGACTTGGGTGTCCTCGCCGCCGCGCTCTACCGAAAATCCAAGGGCCATCTTCGGATTCTCGACGCATTGTGCGGTTGTGGGATCCGGTCGCTTCGGTACTTGGTCGAGTCCGAGGCTGATTTTGTGTTGGCGAATGACGCTAATGATGAAAATCGGAGGGTTATTGTGAAGAATCTCTCGAAAGTTGCCGAAAATTTTGGGGATAATCGACGGTGGTTGGTTACTAATTGTGATGCGAACAGGGTAATGACGGAGTTTTACTTGAAGAAGGATTTCTTTGATTTTATTGATGTTGATTCCTTTGGAAGTGATTCGTCGTTTCTGAGACCCGCTATAAATGCTTTGAAATTGGATGGATTGCTCTATGTTACGTCCACGGATGGTTTCTCTTCCGGCGGACATCGGCCTTCTCAATCTTTAGCTGCATATGGAGCATATATACGTCCCATGCCATTTTCAAATGAAATTGGTCTGCGGATGCTTATAGGTGGGGTTGCACGTGAAGCTTCTGTACTGGGTTACTATGCTACTCCACTCTTTTCGTATTATTCGTACCATGGACCTGTTTTCAGAGTCATGCTACGAATCAATCGAGGGAGGATTTCTAAAAACAGGCATTATGGTTTTGTCAGCTATTGCGAAAAATGTGGAAATTCACAAGCTTTTTCATGGAGTGAGCTTGGACAGATGAGCTGTCCTTGCAATGATTCAAGGGTTTCTGAGTCACTAGTGGTCTCAGGACCTCTTTGGATTGGACCTCTACACAGTGCAGACTACATTGAAGACATATTAACCTTGGCCAAGCAATGGGAATGGATAGGCAATGGCCAGGGGAAGGATCTTGAGAAACTTCTGATGCAGATGCTGGATGAGAGTGACTCCAAACTACCAGTAGGGTACATCAAAATGGACGAGGTGGCAAGTCGGGCAAAAGTAAACTCACCTCCCCTCGGTACCATGATGAGCGAGATGATCAAGGCTGGATATGCTGCTAGTAGGTCTCATATTGCCTCAAACGCAATCAAGACTAACTGTCCTATGGCAGACTGCATCAGAATTGCTCAACTGCAGCAAAATTGTCTTGGTGTGCATCAAGTCACATGAATGTTGTAGCCATAGCTTGGGGCTATGGAAACTACAACCCTTCATACGCAAACAAGAACGAAATGAAACCAATGTTTTGGTGACGAGAGACAACCAAGTTAGATCGAAAAACTTTAACTTTCTGTTGCATTCTGTCAAAGATAGACAAGAAATGAGCACATACAGACACAAGACAGGGTCACAGAGACACCATGCCATTTCATTTAAAGTGTCGTATTGATGCTTTATAGAAGTAGATAACTAAAATGTTTATATCAAATTCATGCATATTAAGTGTAAATTAATTAGTTTATTATATGAGCCAGGATGAGATCACTGAAGCAGTTGAACTTCGATTTTGAAGAACGAAAGCTGTGCGGAGCTCAAGTACGGGACTTGATAAAGAACCTTCCCAGACTTGTTAGCCACAACTCTTTATTTATACTTCTTATAGAGCCATGATGTGATGTGATGTGATGTTCTCATCTTAGGCGCAAAATGAAATATTAAGCTAGAAAGTGCAGGCCAATAGTAAAATCTACCTTCTCAGTTTTTAGTTGAACATTGCTGACAAGACTAACCTAATATGTAGGGATATATTATGAAATTTTCAATTTTTGTGTGCAACAGGAAAAGTAACTCAATCATAAATGGCATAGTCTACCTCCTTGTAGGTTCAAGATTATATCCCTATTCCTGTGATTGTCGTACCAAAGCAAATTGTGTGTATAATAGAATATTGTTGCAAAATTCACATGCACCTAAAGCACGCTGACTAAATTTGTAAGGATTATGGGAAAAGCTGGTATCGAATGGGTTTAATGGATTTTTGCAAGTTTGTTTGCCTCGTTCCTTCACTATGCCAAACACTAAAATTCACCTAAGA

Coding sequence (CDS)

ATGTTAACTGTAGCGTCAACAACCCTATCTCCTTCCCCGTTTCTTTACATCCAACAAACAAACTCCCATTTCCTCACCTCTCCATCCATTCATCTCTTCCAATTTTCACACTCCAACAAAAAATCCCCCAACAATTGTCTCTCAAACAATCGCACCGAAAGGGGCTTAGATTTCGACATCGGAGACACCTTCTTCCGCCATGAAAGCGCCACCGGACGCGACTTGGGTGTCCTCGCCGCCGCGCTCTACCGAAAATCCAAGGGCCATCTTCGGATTCTCGACGCATTGTGCGGTTGTGGGATCCGGTCGCTTCGGTACTTGGTCGAGTCCGAGGCTGATTTTGTGTTGGCGAATGACGCTAATGATGAAAATCGGAGGGTTATTGTGAAGAATCTCTCGAAAGTTGCCGAAAATTTTGGGGATAATCGACGGTGGTTGGTTACTAATTGTGATGCGAACAGGGTAATGACGGAGTTTTACTTGAAGAAGGATTTCTTTGATTTTATTGATGTTGATTCCTTTGGAAGTGATTCGTCGTTTCTGAGACCCGCTATAAATGCTTTGAAATTGGATGGATTGCTCTATGTTACGTCCACGGATGGTTTCTCTTCCGGCGGACATCGGCCTTCTCAATCTTTAGCTGCATATGGAGCATATATACGTCCCATGCCATTTTCAAATGAAATTGGTCTGCGGATGCTTATAGGTGGGGTTGCACGTGAAGCTTCTGTACTGGGTTACTATGCTACTCCACTCTTTTCGTATTATTCGTACCATGGACCTGTTTTCAGAGTCATGCTACGAATCAATCGAGGGAGGATTTCTAAAAACAGGCATTATGGTTTTGTCAGCTATTGCGAAAAATGTGGAAATTCACAAGCTTTTTCATGGAGTGAGCTTGGACAGATGAGCTGTCCTTGCAATGATTCAAGGGTTTCTGAGTCACTAGTGGTCTCAGGACCTCTTTGGATTGGACCTCTACACAGTGCAGACTACATTGAAGACATATTAACCTTGGCCAAGCAATGGGAATGGATAGGCAATGGCCAGGGGAAGGATCTTGAGAAACTTCTGATGCAGATGCTGGATGAGAGTGACTCCAAACTACCAGTAGGGTACATCAAAATGGACGAGGTGGCAAGTCGGGCAAAAGTAAACTCACCTCCCCTCGGTACCATGATGAGCGAGATGATCAAGGCTGGATATGCTGCTAGTAGGTCTCATATTGCCTCAAACGCAATCAAGACTAACTGTCCTATGGCAGACTGCATCAGAATTGCTCAACTGCAGCAAAATTGTCTTGGTGTGCATCAAGTCACATGA

Protein sequence

MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDIGDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDANDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAREASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQMLDESDSKLPVGYIKMDEVASRAKVNSPPLGTMMSEMIKAGYAASRSHIASNAIKTNCPMADCIRIAQLQQNCLGVHQVT

Homology

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BLAST of Cmc10g0277441 vs. NCBI nr
Match: XP_008456934.1 (PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Cucumis melo] >XP_008456936.1 PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Cucumis melo] >TYK22417.1 tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 889.0 bits (2296), Expect = 1.6e-254
Identity = 439/440 (99.77%), Postives = 439/440 (99.77%), Query Frame = 0


Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
           MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI
Sbjct: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300
           EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360
           GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360

Query: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLGTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MLDESDSKLPVGYIKMDEVASRAKVNSPPL TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 ADCIRIAQLQQNCLGVHQVT 441
           ADCIRIAQLQQNCLGVHQVT
Sbjct: 421 ADCIRIAQLQQNCLGVHQVT 440

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BLAST of Cmc10g0277441 vs. NCBI nr
Match: XP_011655038.1 (tRNA (guanine(26)-N(2))-dimethyltransferase [Cucumis sativus] >XP_011655039.1 tRNA (guanine(26)-N(2))-dimethyltransferase [Cucumis sativus] >KGN50729.1 hypothetical protein Csa_005829 [Cucumis sativus])

HSP 1 Score: 849.7 bits (2194), Expect = 1.1e-242
Identity = 419/440 (95.23%), Postives = 427/440 (97.05%), Query Frame = 0


Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
           MLTVA  TLSPSPFL+I QTNSHFLTS SIHLFQFSHSNK   NNCLSNNRTERGLDFDI
Sbjct: 1   MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG LR+LDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDENRRVIVKNLS+VAEN GDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAY+RPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300
           EASVLG+YATPLFSYYSYHGPVFRVMLRINRGRI KNRHYGFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360
           GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 360

Query: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLGTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           M+DESDSKLPVGYIKMDEVASRAKVNSPPL TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 ADCIRIAQLQQNCLGVHQVT 441
           A+CIRIAQLQ+NCLG HQVT
Sbjct: 421 AECIRIAQLQRNCLGAHQVT 440

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BLAST of Cmc10g0277441 vs. NCBI nr
Match: XP_008456937.1 (PREDICTED: tRNA (guanine(26)-N(2)/guanine(27)-N(2))-dimethyltransferase isoform X2 [Cucumis melo])

HSP 1 Score: 807.0 bits (2083), Expect = 8.1e-230
Identity = 398/399 (99.75%), Postives = 398/399 (99.75%), Query Frame = 0


Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
           MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI
Sbjct: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300
           EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360
           GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360

Query: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLGTMMSEMIK 400
           MLDESDSKLPVGYIKMDEVASRAKVNSPPL TMMSEMIK
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIK 399

[Close]

BLAST of Cmc10g0277441 vs. NCBI nr
Match: XP_038892632.1 (tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Benincasa hispida])

HSP 1 Score: 806.2 bits (2081), Expect = 1.4e-229
Identity = 395/441 (89.57%), Postives = 420/441 (95.24%), Query Frame = 0


Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
           ML+V   T SPSPFL+  QTNSH L++ +IHLFQFSHSNK S NNCL NNRTERGL+FDI
Sbjct: 1   MLSVLPRTPSPSPFLHSPQTNSHSLSASTIHLFQFSHSNKNSLNNCLPNNRTERGLEFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
           G TFFRHESATGRDLGVLAAALYRKSKGHLR+LDALCGCGIRSLRYLVE+EADFVLANDA
Sbjct: 61  GGTFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDENRRVIVKNLS+VAE+ GD RRW+VTNCDANRVMT FYL+KDFFDFID+DSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSRVAEDSGDARRWMVTNCDANRVMTGFYLQKDFFDFIDIDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAY+RPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300
           EASVLG+YATPLFSYYSYHGPVFRVMLRINRGR+ KNRHYGF+SYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGHYATPLFSYYSYHGPVFRVMLRINRGRVYKNRHYGFISYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360
           GQMSCPCNDSRVS+SLVVSGPLW GPLHSADYIE++L+LAKQW+WIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSDSLVVSGPLWTGPLHSADYIENMLSLAKQWKWIGNGQGKDLEKLLGQ 360

Query: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLGTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           M+DESDSKLPVGYIKMDEVASRAKVNSPPL TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MMDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 ADCIRIA-QLQQNCLGVHQVT 441
           A+CIRIA +LQQNCLG HQVT
Sbjct: 421 AECIRIALELQQNCLGFHQVT 441

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BLAST of Cmc10g0277441 vs. NCBI nr
Match: XP_022943113.1 (tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita moschata])

HSP 1 Score: 797.3 bits (2058), Expect = 6.4e-227
Identity = 392/441 (88.89%), Postives = 415/441 (94.10%), Query Frame = 0


Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
           MLTV   TLSPSPFL  QQT SH  +S +IHLFQFS S KK  NNCL NNRTERG++FDI
Sbjct: 1   MLTVMLNTLSPSPFLRTQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG LR+LDALCGCGIRSLRYLVE+EADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDENR VIVKNLS+VAE FG+ RRW+VTNCDANRVM+EFYL+KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGH PSQSLAAYGAY+RPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300
           EASVLG+Y TPLFSYYSYHGPVFRVMLRINRGRI+KNRHYGFVSYC+KCGNSQAFSWSE+
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRINRGRINKNRHYGFVSYCKKCGNSQAFSWSEV 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360
           GQMSCPCND+RVSESLVVSGPLW GPLHSADYIED+LTLA QWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360

Query: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLGTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           M+DESDSKLPVGYIKMDEVASRAKVNSPPL TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 ADCIRIA-QLQQNCLGVHQVT 441
           A+CIRIA +LQQ+CLGVHQ T
Sbjct: 421 AECIRIALELQQSCLGVHQAT 441

[Close]

BLAST of Cmc10g0277441 vs. ExPASy Swiss-Prot
Match: P81554 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) OX=186497 GN=trm1 PE=1 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 4.3e-32
Identity = 109/369 (29.54%), Postives = 172/369 (46.61%), Query Frame = 0


Query: 64  FFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDANDE 123
           F+    A  RD+ VL   + +       +LDAL   GIR +R+ +E+ A+ +  ND N+ 
Sbjct: 29  FYNPRMAPNRDVVVLLLNVLKPK----IVLDALSATGIRGIRFALETPAEEIWMNDINEL 88

Query: 124 NRRVIVKNL-----SKVAEN-----FGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDS 183
              ++ KN+       + EN     F   +  ++ N DANR+M E   K  +F FID+D 
Sbjct: 89  AYELMKKNVLLNFKGTLKENAKRAIFEGEKTIVINNDDANRLMAE---KHRYFHFIDLDP 148

Query: 184 FGSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRM 243
           FGS   FL  A+ ++K  G+L VT+TDG    G  P   L  Y A        +E+G R+
Sbjct: 149 FGSPMEFLDTALRSVKRKGILGVTATDGAPLCGAHPKACLRKYLAVPLRGELCHEVGTRI 208

Query: 244 LIGGVAREASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKN---RHYGFVSYCEKCG 303
           L+G +AR A+        L +YY  H   FR  +++  G    +    + G+V + EK G
Sbjct: 209 LVGVIARYAAKYDLGMEVLLAYYKDH--YFRAFVKLKDGAKKGDETLENLGYVYFDEKTG 268

Query: 304 NSQAFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQ 363
             +                S +S      GPLW+GPL +   + ++L L      + N  
Sbjct: 269 RFEV-------------EKSFLSTRPNAYGPLWLGPLKNEKVVGEMLELLVSGFEVAN-- 328

Query: 364 GKDLEKLLMQMLDESDSKLPVGYIKMDEVASRAKVNSPPLGTMMSEMIKAGYAASRSHIA 420
            +++ KLL  + +E D  L   +     +  R K+    LG ++ E+   GY A+R+H +
Sbjct: 329 YREVLKLLHMLHEELDIPL---FYDTHALGKRLKIEPKKLGEIIKELKSMGYEATRTHFS 370

[Close]

BLAST of Cmc10g0277441 vs. ExPASy Swiss-Prot
Match: B6YUU9 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus onnurineus (strain NA1) OX=523850 GN=trm1 PE=3 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 1.2e-31
Identity = 114/373 (30.56%), Postives = 183/373 (49.06%), Query Frame = 0


Query: 64  FFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDANDE 123
           F+    A  RD+ VLA     K+ G  R+LDAL   GIR +RY +E+ A  +  ND N  
Sbjct: 27  FYNPLMALNRDISVLAL----KALGSRRVLDALSATGIRGIRYALETPAGEIWLNDINPN 86

Query: 124 NRRVIVKNL-----------SKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVD 183
             R+IV N+            K+A   G+ +  +VTN DANR+M E +    +FDF+D+D
Sbjct: 87  AFRLIVDNVKLNFSGELEIEGKMAVLEGE-KTLVVTNADANRLMNEQF---RYFDFVDLD 146

Query: 184 SFGSDSSFLRPAINALKLDGLLYVTSTD-GFSSGGHRPSQSLAAYGAYIRPMPFSNEIGL 243
            FGS   FL  A+ A+K  G+L +T+TD G   G +R +  L  Y A        +E GL
Sbjct: 147 PFGSPMEFLDSALRAVKRRGVLAITATDTGVLCGAYR-NACLRKYLAEPIRGELCHEAGL 206

Query: 244 RMLIGGVAREASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGN 303
           R+LIG + R A+        L +YY  H   FR  L++  G  + ++    + Y  +   
Sbjct: 207 RILIGTLVRYAAKYDLGVEVLLAYYRDH--YFRAFLQLRNGAKTADKSMKQLGYLYQ--- 266

Query: 304 SQAFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQG 363
                  E G+      ++ +       GPLW+GPL + +++E++  L+ +   +G+   
Sbjct: 267 ------DESGKFE--FENTFLPSKATAHGPLWLGPLKNQEFVEELHRLSAEHP-LGS--- 326

Query: 364 KDLEKLLMQMLDESDSKLPVGYIKMDEVASRAKVNSPPLGTMMSEMIKAGYAASRSHIAS 423
           K  +K L  + DE +  +P  Y     VA R  +    L  ++  + + GY A+R+H + 
Sbjct: 327 KKTQKFLGLLKDELE--VPFFY-DTHAVARRHGLEVRKLAGVIEILYEKGYKATRTHFSP 370

Query: 424 NAIKTNCPMADCI 425
            A+KT+ P  + +
Sbjct: 387 TALKTDAPFEEVL 370

[Close]

BLAST of Cmc10g0277441 vs. ExPASy Swiss-Prot
Match: Q9V1P3 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus abyssi (strain GE5 / Orsay) OX=272844 GN=trm1 PE=3 SV=2)

HSP 1 Score: 136.0 bits (341), Expect = 1.1e-30
Identity = 101/369 (27.37%), Postives = 178/369 (48.24%), Query Frame = 0


Query: 64  FFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDANDE 123
           F+    A  RD+ V+   + +       +LDAL   GIR +R+ +E+ A+ +  ND +++
Sbjct: 27  FYNPRMALNRDIAVVLLNVLKPR----IVLDALSATGIRGIRFALETPAEEIWMNDISED 86

Query: 124 NRRVIVKNL-----SKVAENFG-----DNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDS 183
              ++ KN+      ++ E+ G       +  +V + DANR+M E   K  +F FID+D 
Sbjct: 87  AYNLMKKNVLLNFKGELEESNGRAVLKSEKTLVVNHDDANRLMAE---KHRYFHFIDLDP 146

Query: 184 FGSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRM 243
           FGS   FL  A+ ++K  G+L +T+TDG    G  P   +  Y A        +E+G R+
Sbjct: 147 FGSPMEFLDTALRSVKRKGILGITATDGAPLCGAHPKACMRKYLAVPLRGELCHEVGTRI 206

Query: 244 LIGGVAREASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKN---RHYGFVSYCEKCG 303
           L+G VAR A+        + +YY  H   FR  +++  G    +    + G++ + E  G
Sbjct: 207 LVGVVARYAAKYDLGIEVILAYYKDH--YFRAFIKLKDGARKGDESLENLGYIYFDESTG 266

Query: 304 NSQAFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQ 363
                   E+ +   P   +         GP+W+GPL S + +E++L +++Q   +   +
Sbjct: 267 K------FEVERSFLPSKPN-------AYGPVWLGPLKSQEIVEEMLEISQQ---LSLAR 326

Query: 364 GKDLEKLLMQMLDESDSKLPVGYIKMDEVASRAKVNSPPLGTMMSEMIKAGYAASRSHIA 420
            K   KLL  + DE D  L   +     +  R K+ +  +  +++E+   GY ASR+H +
Sbjct: 327 KKQAVKLLKILKDELDVPL---FYDTHGLGRRLKIEARKIEEIINELRSLGYRASRTHFS 367

[Close]

BLAST of Cmc10g0277441 vs. ExPASy Swiss-Prot
Match: Q97ZH0 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) OX=273057 GN=trm1 PE=3 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 3.1e-30
Identity = 105/360 (29.17%), Postives = 186/360 (51.67%), Query Frame = 0


Query: 64  FFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVES-EADFVLANDAND 123
           F+  +    RDL V+  ++ +       I+DAL   GIR +RY VES +++ ++ ND N 
Sbjct: 35  FYNPKMTFNRDLSVIVVSILKPK----IIVDALSATGIRGIRYYVESWKSEQLILNDKNP 94

Query: 124 ENRRVIVKNLSKVAENFG-DNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSFL 183
               +I  N    A+N G +N +  + N DAN ++ E        D+ID+D FGS + F+
Sbjct: 95  NATSLIQIN----AKNNGIENAK--IYNKDANALLYEVK-----SDYIDIDPFGSPAPFI 154

Query: 184 RPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVARE 243
             ++NA   +G++  T+TD     G  P+     Y A    +  S E+GLR+LIG + RE
Sbjct: 155 LSSLNAATRNGIVAFTATDLSPLEGSSPTSCRRKYDAINHKLSSSKELGLRVLIGKIIRE 214

Query: 244 ASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISK----NRHYGFVSYCEKCGNSQAFSW 303
           A++L     PLFS+Y+ +   +R+ +R+  G        N+H  +   C +CG  Q F  
Sbjct: 215 AAILEKTVYPLFSFYADY--YYRLFVRVENGARKADDNINKHLKYFGECPRCG-FQTFV- 274

Query: 304 SELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKL 363
            E  +  CP       E   + GPL+IGPL+S ++++ I+ L   + ++ +     ++KL
Sbjct: 275 EENCKTKCPV----CGEIFSIIGPLYIGPLYSMEFLKRIMDLYSNFNYLTS--FNRIQKL 334

Query: 364 LMQMLDESDSKLPVGYIKMDEVASRAKVNSPPLGTMMSEMIKAGYAASRSHIASNAIKTN 418
           L  +  E +++    +  + ++AS+ K+++ P    +  +++    ASR+H A   I+T+
Sbjct: 335 LNVI--EKEARFKNVFYNISKLASKLKISAIP---PIESILECLGDASRTHFAPTGIRTD 364

[Close]

BLAST of Cmc10g0277441 vs. ExPASy Swiss-Prot
Match: C5A6N2 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3) OX=593117 GN=trm1 PE=3 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 9.9e-29
Identity = 113/379 (29.82%), Postives = 176/379 (46.44%), Query Frame = 0


Query: 64  FFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDANDE 123
           F+    A  RD+ VLA     K+    R+LDAL   GIR LRY +E+ A+ V  ND N++
Sbjct: 27  FYNPVMALNRDISVLAV----KALSPRRVLDALSATGIRGLRYSLETSAEEVWMNDINED 86

Query: 124 NRRVIVKNLSKVAENFGD--------------NRRWLVTNCDANRVMTEFYLKKDFFDFI 183
             R+I+KNL     NFG                +R +V   DAN++M+E +    +FDFI
Sbjct: 87  AFRLILKNLEL---NFGSKPVLANGRAELGLPEKRLVVNLGDANKLMSENF---RYFDFI 146

Query: 184 DVDSFGSDSSFLRPAINALKLDGLLYVTSTD-GFSSGGHRPSQSLAAYGAYIRPMPFSNE 243
           D+D FGS   FL  A+ +++  G L +T+TD G   G +R +         IR     +E
Sbjct: 147 DLDPFGSPVEFLDSALRSVRRRGFLGLTATDTGVLCGAYRRACRRKYLAEPIRG-ELCHE 206

Query: 244 IGLRMLIGGVAREASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEK 303
            GLR+LIG + R A+        L +YY  H   FRV LR+  G    +     + Y   
Sbjct: 207 AGLRILIGTIVRYAAKYDLGVEVLLAYYRDH--YFRVFLRLRSGAKKADESVELLGYL-- 266

Query: 304 CGNSQAFSW-SELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAK-QWEWI 363
                   W  E G+         +       GP+W+GPL +  ++E+++ L K   E I
Sbjct: 267 --------WQDETGKFK--YERDFLPGKRGAYGPMWLGPLKNQAFLEELVRLTKTSEESI 326

Query: 364 GNGQGKDLEKLLMQMLDESDSKLPVGYIKMDEVASRAKVNSPPLGTMMSEMIKAGYAASR 423
            + +      LL++ LD     +P  Y     +A +  +    +  ++  +   G+ A+R
Sbjct: 327 SHRKTFQFLSLLVEELD-----VPFHY-DTHALARKNGLEVVKISRVIETLHSLGFEATR 374

Query: 424 SHIASNAIKTNCPMADCIR 426
           +H +  +IKTN P    +R
Sbjct: 387 THFSPTSIKTNAPFDVVLR 374

[Close]

BLAST of Cmc10g0277441 vs. ExPASy TrEMBL
Match: A0A1S3C509 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103496736 PE=3 SV=1)

HSP 1 Score: 889.0 bits (2296), Expect = 7.9e-255
Identity = 439/440 (99.77%), Postives = 439/440 (99.77%), Query Frame = 0


Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
           MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI
Sbjct: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300
           EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360
           GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360

Query: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLGTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MLDESDSKLPVGYIKMDEVASRAKVNSPPL TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 ADCIRIAQLQQNCLGVHQVT 441
           ADCIRIAQLQQNCLGVHQVT
Sbjct: 421 ADCIRIAQLQQNCLGVHQVT 440

[Close]

BLAST of Cmc10g0277441 vs. ExPASy TrEMBL
Match: A0A5D3DFE8 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold530G00120 PE=3 SV=1)

HSP 1 Score: 889.0 bits (2296), Expect = 7.9e-255
Identity = 439/440 (99.77%), Postives = 439/440 (99.77%), Query Frame = 0


Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
           MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI
Sbjct: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300
           EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360
           GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360

Query: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLGTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MLDESDSKLPVGYIKMDEVASRAKVNSPPL TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 ADCIRIAQLQQNCLGVHQVT 441
           ADCIRIAQLQQNCLGVHQVT
Sbjct: 421 ADCIRIAQLQQNCLGVHQVT 440

[Close]

BLAST of Cmc10g0277441 vs. ExPASy TrEMBL
Match: A0A0A0KP99 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G220920 PE=3 SV=1)

HSP 1 Score: 849.7 bits (2194), Expect = 5.3e-243
Identity = 419/440 (95.23%), Postives = 427/440 (97.05%), Query Frame = 0


Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
           MLTVA  TLSPSPFL+I QTNSHFLTS SIHLFQFSHSNK   NNCLSNNRTERGLDFDI
Sbjct: 1   MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG LR+LDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDENRRVIVKNLS+VAEN GDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAY+RPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300
           EASVLG+YATPLFSYYSYHGPVFRVMLRINRGRI KNRHYGFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360
           GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 360

Query: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLGTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           M+DESDSKLPVGYIKMDEVASRAKVNSPPL TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 ADCIRIAQLQQNCLGVHQVT 441
           A+CIRIAQLQ+NCLG HQVT
Sbjct: 421 AECIRIAQLQRNCLGAHQVT 440

[Close]

BLAST of Cmc10g0277441 vs. ExPASy TrEMBL
Match: A0A1S3C4F5 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103496736 PE=3 SV=1)

HSP 1 Score: 807.0 bits (2083), Expect = 3.9e-230
Identity = 398/399 (99.75%), Postives = 398/399 (99.75%), Query Frame = 0


Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
           MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI
Sbjct: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300
           EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360
           GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360

Query: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLGTMMSEMIK 400
           MLDESDSKLPVGYIKMDEVASRAKVNSPPL TMMSEMIK
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIK 399

[Close]

BLAST of Cmc10g0277441 vs. ExPASy TrEMBL
Match: A0A6J1FWD4 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111447941 PE=3 SV=1)

HSP 1 Score: 797.3 bits (2058), Expect = 3.1e-227
Identity = 392/441 (88.89%), Postives = 415/441 (94.10%), Query Frame = 0


Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60
           MLTV   TLSPSPFL  QQT SH  +S +IHLFQFS S KK  NNCL NNRTERG++FDI
Sbjct: 1   MLTVMLNTLSPSPFLRTQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG LR+LDALCGCGIRSLRYLVE+EADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDENR VIVKNLS+VAE FG+ RRW+VTNCDANRVM+EFYL+KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGH PSQSLAAYGAY+RPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300
           EASVLG+Y TPLFSYYSYHGPVFRVMLRINRGRI+KNRHYGFVSYC+KCGNSQAFSWSE+
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRINRGRINKNRHYGFVSYCKKCGNSQAFSWSEV 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360
           GQMSCPCND+RVSESLVVSGPLW GPLHSADYIED+LTLA QWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360

Query: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLGTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           M+DESDSKLPVGYIKMDEVASRAKVNSPPL TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 ADCIRIA-QLQQNCLGVHQVT 441
           A+CIRIA +LQQ+CLGVHQ T
Sbjct: 421 AECIRIALELQQSCLGVHQAT 441

[Close]

BLAST of Cmc10g0277441 vs. TAIR 10
Match: AT3G56330.1 (N2,N2-dimethylguanosine tRNA methyltransferase )

HSP 1 Score: 553.9 bits (1426), Expect = 1.2e-157
Identity = 270/430 (62.79%), Postives = 337/430 (78.37%), Query Frame = 0


Query: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCL--SNNRTERGLDF 60
           +LT++  TLS S F  + ++ +    SP    F+   S K S +  L  S  + ER L+F
Sbjct: 2   LLTLSPKTLSSSSFFTVHKSQNPKCKSPDSCRFK---SLKCSFDRILVKSEVQIERNLEF 61

Query: 61  DIGDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLAN 120
           + G+TFFRHESA GRDLGVL+A LY++S G LR+LDA+CGCGIRSLRYLVE+EADFV+AN
Sbjct: 62  ETGETFFRHESARGRDLGVLSATLYKRSNGSLRVLDAMCGCGIRSLRYLVEAEADFVMAN 121

Query: 121 DANDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDS 180
           DAND+NRRVI  NLSKV    GD RRW+VT+  AN+ M E Y+  DFFD ID+DSFGSDS
Sbjct: 122 DANDDNRRVITDNLSKVERGTGDERRWVVTHMLANKAMIERYMVADFFDMIDIDSFGSDS 181

Query: 181 SFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGV 240
           SFLR A NAL+L GLLY+TSTDG+SSGGHRP  SLAAYGA+IRPMPF NEIGLRMLIGG 
Sbjct: 182 SFLRDAFNALRLGGLLYLTSTDGYSSGGHRPYNSLAAYGAFIRPMPFGNEIGLRMLIGGA 241

Query: 241 AREASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWS 300
            REA++LGY+ TPLFSYYSYHGPVFRVMLR++RG++ ++R+YGFV++C  CG+S    + 
Sbjct: 242 VREAALLGYHVTPLFSYYSYHGPVFRVMLRVHRGKLHEDRNYGFVTHCNLCGHSHTLRFD 301

Query: 301 ELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLL 360
           ELG M CPC+D++ S SLVVSGP+W+GPLH A Y+ ++L LAK+W W+  G G DL+KLL
Sbjct: 302 ELGLMGCPCSDTKASSSLVVSGPMWLGPLHDASYVTEMLELAKEWGWVSEGTGMDLDKLL 361

Query: 361 MQMLDESDSKLPVGYIKMDEVASRAKVNSPPLGTMMSEMIKAGYAASRSHIASNAIKTNC 420
             M++ESD +LP GY KMDE+ASRAK+NSPPL  MMS ++K GYAASRSHI  NA+KT+C
Sbjct: 362 SIMIEESDPRLPPGYTKMDEMASRAKMNSPPLKKMMSALVKEGYAASRSHIIPNALKTDC 421

Query: 421 PMADCIRIAQ 429
           PM+  +RIA+
Sbjct: 422 PMSHFVRIAK 428

[Close]

BLAST of Cmc10g0277441 vs. TAIR 10
Match: AT3G02320.1 (N2,N2-dimethylguanosine tRNA methyltransferase )

HSP 1 Score: 87.8 bits (216), Expect = 2.3e-17
Identity = 85/355 (23.94%), Postives = 147/355 (41.41%), Query Frame = 0


Query: 91  RILDALCGCGIRSLRYLVESEA-DFVLANDANDENRRVIVKN--------LSKVAENFGD 150
           ++L+AL   G+R+LRY  E E    V+A D +  +     +N        +SKV  +  D
Sbjct: 123 KVLEALSASGLRALRYAREIEGIGQVVALDNDLASVEACQRNIKFNGSVAISKVESHHTD 182

Query: 151 NRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSFLRPAINALKLDGLLYVTSTDG 210
            R  ++T+                FD +D+D +GS S FL  AI ++   GLL  T+TD 
Sbjct: 183 ARVHMLTH-------------PKEFDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDM 242

Query: 211 FSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAREASVLGYYATPLFSYYSYHGP 270
               G       + YG+Y     + +E+ LR+L+  +   A+    Y  P+ S       
Sbjct: 243 AVLCGGNGEVCYSKYGSYPLRAKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDF-- 302

Query: 271 VFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSELGQMSCPCNDS----------- 330
             RV +R+     +       +SY  +C    +F    +G+ S P N+S           
Sbjct: 303 YVRVFVRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQPVGR-SLPKNNSVRYLPAIGPVV 362

Query: 331 -----RVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQMLDES 390
                   +   + GP+W  P+H  +++  IL   K  +        D    ++  + E 
Sbjct: 363 KQDCNHCGKKYNMGGPIWSAPMHDPEWVTSILNSVKSMK--DRYPAYDRISAVLTTVSEE 422

Query: 391 DSKLPVGYIKMDEVASRAKVNSPPLGTMMSEMIKAGYAASRSHIASNAIKTNCPM 421
              +P+ ++ +  + +  K  SP      S +I A Y  S +H+    +KT+ PM
Sbjct: 423 LLDVPL-FLSLHNLCATLKCISPSAAMFRSAVINANYRISGTHVNPLGMKTDAPM 458

The following BLAST results are available for this feature:

BLAST of Cmc10g0277441 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008456934.1	1.6e-254	99.77	PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Cucumis melo]...	[more]
XP_011655038.1	1.1e-242	95.23	tRNA (guanine(26)-N(2))-dimethyltransferase [Cucumis sativus] >XP_011655039.1 tR...	[more]
XP_008456937.1	8.1e-230	99.75	PREDICTED: tRNA (guanine(26)-N(2)/guanine(27)-N(2))-dimethyltransferase isoform ...	[more]
XP_038892632.1	1.4e-229	89.57	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Benincasa hispida]	[more]
XP_022943113.1	6.4e-227	88.89	tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita moschata]	[more]

BLAST of Cmc10g0277441 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P81554	4.3e-32	29.54	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus furiosus (strain ATCC ...	[more]
B6YUU9	1.2e-31	30.56	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus onnurineus (strain N...	[more]
Q9V1P3	1.1e-30	27.37	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus abyssi (strain GE5 / O...	[more]
Q97ZH0	3.1e-30	29.17	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Saccharolobus solfataricus (strai...	[more]
C5A6N2	9.9e-29	29.82	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus gammatolerans (strai...	[more]

BLAST of Cmc10g0277441 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3C509	7.9e-255	99.77	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103496...	[more]
A0A5D3DFE8	7.9e-255	99.77	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A0A0KP99	5.3e-243	95.23	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G...	[more]
A0A1S3C4F5	3.9e-230	99.75	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103496...	[more]
A0A6J1FWD4	3.1e-227	88.89	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC...	[more]

BLAST of Cmc10g0277441 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G56330.1	1.2e-157	62.79	N2,N2-dimethylguanosine tRNA methyltransferase	[more]
AT3G02320.1	2.3e-17	23.94	N2,N2-dimethylguanosine tRNA methyltransferase	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 33..367 e-value: 2.9E-74 score: 251.7
None	No IPR available	PANTHER	PTHR10631:SF9	TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE	coord: 26..431
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 91..197 e-value: 7.66957E-5 score: 39.7207
IPR002905	tRNA methyltransferase, Trm1	PFAM	PF02005	TRM	coord: 64..426 e-value: 4.3E-65 score: 220.3
IPR002905	tRNA methyltransferase, Trm1	PANTHER	PTHR10631	N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE	coord: 26..431
IPR002905	tRNA methyltransferase, Trm1	PROSITE	PS51626	SAM_MT_TRM1	coord: 49..427 score: 92.280968
IPR042296	tRNA methyltransferase, Trm1, C-terminal	GENE3D	3.30.56.70		coord: 372..428 e-value: 2.0E-15 score: 58.2
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 52..427

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cmc10g0277441.1	Cmc10g0277441.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008033	tRNA processing
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0004809	tRNA (guanine-N2-)-methyltransferase activity