Cmc10g0268181 (gene) Melon (Charmono) v1.1

Overview
NameCmc10g0268181
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionPyruvate dehydrogenase E1 component subunit alpha
LocationCMiso1.1chr10: 3300377 .. 3303724 (+)
RNA-Seq ExpressionCmc10g0268181
SyntenyCmc10g0268181
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGTATTTCCTTTTTCAAATAGAATAAAAATAAGAAAAGAAAAGAAGGCCAAAACTTAAGTTTCTAGAGAAAGACACATCGATTTAGCGACTCATCACTTCTTGAACAGCCAACTTTCTTTCTTTTTCCTTTTTCCTTTTTCCTTTTTCCAATTTTTACATTACTATTTATCCTTCACATATTTTTAATATTCCCGCCTCCTGCCGGTCACCGCCACGACCCTACGCTCACTCTACCACCGTCGGCATGTCTCATTCTCCTATTTCTTCTTCCTCCATTCTCAAACCTCTCTCTTCTTCTTTCCGTTTCCTTTCATCTGCCGCCCATGACTCCGCCCACCTCACAATCCAAACTTCCGTTCCCTTCACCGCTCACAAATGCGAGCCACCATCGCGTTCCGTGGACACCAGTCCCAACGAGTTACTTGCTTTCTTTCGGGAAATGGCACTTATGCGACGCATGGAAATCGCCGCCGACTCGCTTTATAAAGCCAAATTGATTCGAGGATTCTGCCATCTCTACGACGGTCAGGAAGCAGTGGCTGTTGGAATGGAGGCTGCAATCACCAAGAAGGACGCCATTATCACTGCTTATCGGGATCACTGCACCTTCCTCGGCCGTGGGGGAACGCTTCTGGAGGCATTCGCCGAACTCATGGGCCGTCAAGTTGGGTGCTCTAAAGGAAAAGGGGGTTCGATGCATTTCTATAAGAAGGACGCTGGGTTCTATGGCGGGCATGGAATAGTGGGGGCTCAAGTGCCCTTGGGATGTGGCTTGGCTTTTGCTCAGAAGTACTCGAAGGAGGGTACTGTGACCTTTGCTCTTTACGGCGACGGTGCGGCAAATCAGGGCCAGTTGTTCGAGGCGCTCAACATATCTGCTCTTTGGGACTTGCCGGTAATTTTGGTCTGTGAGAATAATCATTGTAAGTAATTGTTAGTTCTTGTCAGTATAATCAGTATTTGTGCTTCTATAATCTGCTATAACAATTGATACTGTATAAATGCTTCTTAGCATTGCAATATGCAATATGAAACATGAAATTGGGTTAGTTTATATCTATATAATGTGTTGTAATGTCTTGATTATCTAGCGTTTCAAACGATCCAGTACAAGCATATTTATGTTATTTTACGTTTTTGACCATAGGTTCTATTGAAGTGTGTTATGTATTACTCTGTTATTTTGATCTTATAGTAAACTGTTCTTACATATGGAAGTAGCTAACACATTAGTGAACCTCTTAAATCTCTATGTTAATTTTCTATTGCTTCATGTTTTCTGATTTCTTCCTTTGTTAATACCGTAACAGAATGAACAAACATATTGATCAGTGAGCTAATATTAATTGTCTTTTGTTGATGCAGATGGTATGGGAACTGCAGAGTGGAGAGCAGCCAAGAGTTCAGCCTATTATACGCGTGGTGACTATGTTCCTGGACTGAAGGTATCATTTTCATTTTAATTAGATGAGAATTGGGAGTTGTAGTTCCTAATGTTACAGATTTTCTACTATGTTTATGATCTTTCCTTTCTTTTCCCCTTATTAAATTATTCAACATCAATTGAATTAGTCGATTCGCTATTGTTCTTGAATCTGTTTTGTACTGTTGTTCAAACTTTAGCTAGTTTGATGTTTATATAGTTTCTTTAAACATGCAACTATTTCTTTTTTTTGTTATAGGTGGATGGCATGGATGCCCTTGCTGTCAAGCAAGCTTGTAAGTTTGCCAAGGAGCATGCGTTAAAGAATGGACCCATTGTAAGTTTATCTTCTTAGATTTAAGATTATTCTGTCTTAATGCATAACGAGCTTTGCTGAACAAAACATGTGTATTGCATATGTTTTAAGTGTGTTCCCATGTAGGAATGTGTTGTTTTTTTTTCCACATTGATAGGAGTACGTGTATGCTTCTGGGTATTTCTAATTATATCGAGTGTTATTCCTATTTTGGCATTTCTAATTTCTGGAGTTTTAGCCCTACGAATAAAACACGTCACTGCCTAATAACTCTCTGTACTCCTGAACTGGCCATACTTCTGCCCCGAGCCAATGTTTTCTGGCTTTGTTTCACTTTATTTTAGAAGCAGTTTCTCCCTGTATAAAAACCTCACTTTATTTTCGAATTAAGCACTATAGGATTTCTCTGGTGATGATGTATAAAGCTTAGTATGTTGGACAATGCCTGCCTTTGTTTTCCTTCCACTTGGAATTTAGTTTATCTCTCTTGAATTTCGATTTCAGATTCTTGAGATGGACACCTACAGGTACCATGGTCATTCTATGTCTGATCCTGGAAGTACCTACCGCACACGTGATGAGATTAGCGGTGTGAGACAGGTCTGATCGGCTTCCGTAATGAACTCATCCCAGCTTCATAACTTCTTTGCTCTGATTTCTAAATAAATTTTCTGATTGATCAATCAGGAACGTGATCCAATTGAAAGGATTAGAAAATTGTTATTATCTCATGAGATAGCCACCGAAAAGGATCTGAAGGTATCTTTAAACTTCTCAGTGATACGTTGATGTTTTAAATTTAGTTTCAAAGAGGAAAAAAAAAAGGATAGACTAAGGGAAAAAAATCGTTTTTGTGTTATTTGATCATCAGGATATTGAGAAGGAAATGAGAAAAAAAGTAGATGAAGCCATTGCTCAAGCCAAGGTAATACATGCAATTATTATCTAGCAAACTTAACAACTATATGGTTAACATCCTTAATCCATAGTAACCCTGCTTTCTTCATCGCTATCTCATTTGTGTTTCCAGGCAAGTCCAATGCCAGATGCGTCAGAACTTTTCACAAACGTGTACGTGAAAGGATTTGAAACTGAGGTAATGTGTTCCATCTCCCATCAACAGAGATAACGAGAAGGAAACCAATATCATTAGAATGTAATCTAAATACCCAGACGGTTTTTTCGTGTCTTTACAGGTGTTTGGAGCAGACCGAAAAGAAGTCAGGGGTGTTCTTCCATAAGTAAGACAAGAGTTAGCATTGTATAGACGACCTTTTCACACGAATGCAGTATACCAACGAAACCAAAACCTTATGGTGAAAAAATACAGAATAACAAACATGTTTTTCTTTTCCATGTTCATGGAAACCACTCTCTGGTTGTATATGATTTGGATCTTTTGAAATATAGGTTTGGTTCTTTTAAGGTTCCTGAATATCTAATAAAAGGAAGAAAAAATCCTGTATTAATACCTACCTTGTATCACTTATTCCTTAAGTTACATTACTTCACTTGTACATAATCAAAATAAAGTTTTTCATGGGGAGAATTGCCCAATCATTTTATTATGCATTACAATGGTTTTAATTTAATTTCTAAAGTTTCCTT

mRNA sequence

TGTATTTCCTTTTTCAAATAGAATAAAAATAAGAAAAGAAAAGAAGGCCAAAACTTAAGTTTCTAGAGAAAGACACATCGATTTAGCGACTCATCACTTCTTGAACAGCCAACTTTCTTTCTTTTTCCTTTTTCCTTTTTCCTTTTTCCAATTTTTACATTACTATTTATCCTTCACATATTTTTAATATTCCCGCCTCCTGCCGGTCACCGCCACGACCCTACGCTCACTCTACCACCGTCGGCATGTCTCATTCTCCTATTTCTTCTTCCTCCATTCTCAAACCTCTCTCTTCTTCTTTCCGTTTCCTTTCATCTGCCGCCCATGACTCCGCCCACCTCACAATCCAAACTTCCGTTCCCTTCACCGCTCACAAATGCGAGCCACCATCGCGTTCCGTGGACACCAGTCCCAACGAGTTACTTGCTTTCTTTCGGGAAATGGCACTTATGCGACGCATGGAAATCGCCGCCGACTCGCTTTATAAAGCCAAATTGATTCGAGGATTCTGCCATCTCTACGACGGTCAGGAAGCAGTGGCTGTTGGAATGGAGGCTGCAATCACCAAGAAGGACGCCATTATCACTGCTTATCGGGATCACTGCACCTTCCTCGGCCGTGGGGGAACGCTTCTGGAGGCATTCGCCGAACTCATGGGCCGTCAAGTTGGGTGCTCTAAAGGAAAAGGGGGTTCGATGCATTTCTATAAGAAGGACGCTGGGTTCTATGGCGGGCATGGAATAGTGGGGGCTCAAGTGCCCTTGGGATGTGGCTTGGCTTTTGCTCAGAAGTACTCGAAGGAGGGTACTGTGACCTTTGCTCTTTACGGCGACGGTGCGGCAAATCAGGGCCAGTTGTTCGAGGCGCTCAACATATCTGCTCTTTGGGACTTGCCGGTAATTTTGGTCTGTGAGAATAATCATTATGGTATGGGAACTGCAGAGTGGAGAGCAGCCAAGAGTTCAGCCTATTATACGCGTGGTGACTATGTTCCTGGACTGAAGGTGGATGGCATGGATGCCCTTGCTGTCAAGCAAGCTTGTAAGTTTGCCAAGGAGCATGCGTTAAAGAATGGACCCATTATTCTTGAGATGGACACCTACAGGTACCATGGTCATTCTATGTCTGATCCTGGAAGTACCTACCGCACACGTGATGAGATTAGCGGTGTGAGACAGGAACGTGATCCAATTGAAAGGATTAGAAAATTGTTATTATCTCATGAGATAGCCACCGAAAAGGATCTGAAGGATATTGAGAAGGAAATGAGAAAAAAAGTAGATGAAGCCATTGCTCAAGCCAAGGCAAGTCCAATGCCAGATGCGTCAGAACTTTTCACAAACGTGTACGTGAAAGGATTTGAAACTGAGGTGTTTGGAGCAGACCGAAAAGAAGTCAGGGGTGTTCTTCCATAAGTAAGACAAGAGTTAGCATTGTATAGACGACCTTTTCACACGAATGCAGTATACCAACGAAACCAAAACCTTATGGTGAAAAAATACAGAATAACAAACATGTTTTTCTTTTCCATGTTCATGGAAACCACTCTCTGGTTGTATATGATTTGGATCTTTTGAAATATAGGTTTGGTTCTTTTAAGGTTCCTGAATATCTAATAAAAGGAAGAAAAAATCCTGTATTAATACCTACCTTGTATCACTTATTCCTTAAGTTACATTACTTCACTTGTACATAATCAAAATAAAGTTTTTCATGGGGAGAATTGCCCAATCATTTTATTATGCATTACAATGGTTTTAATTTAATTTCTAAAGTTTCCTT

Coding sequence (CDS)

ATGTCTCATTCTCCTATTTCTTCTTCCTCCATTCTCAAACCTCTCTCTTCTTCTTTCCGTTTCCTTTCATCTGCCGCCCATGACTCCGCCCACCTCACAATCCAAACTTCCGTTCCCTTCACCGCTCACAAATGCGAGCCACCATCGCGTTCCGTGGACACCAGTCCCAACGAGTTACTTGCTTTCTTTCGGGAAATGGCACTTATGCGACGCATGGAAATCGCCGCCGACTCGCTTTATAAAGCCAAATTGATTCGAGGATTCTGCCATCTCTACGACGGTCAGGAAGCAGTGGCTGTTGGAATGGAGGCTGCAATCACCAAGAAGGACGCCATTATCACTGCTTATCGGGATCACTGCACCTTCCTCGGCCGTGGGGGAACGCTTCTGGAGGCATTCGCCGAACTCATGGGCCGTCAAGTTGGGTGCTCTAAAGGAAAAGGGGGTTCGATGCATTTCTATAAGAAGGACGCTGGGTTCTATGGCGGGCATGGAATAGTGGGGGCTCAAGTGCCCTTGGGATGTGGCTTGGCTTTTGCTCAGAAGTACTCGAAGGAGGGTACTGTGACCTTTGCTCTTTACGGCGACGGTGCGGCAAATCAGGGCCAGTTGTTCGAGGCGCTCAACATATCTGCTCTTTGGGACTTGCCGGTAATTTTGGTCTGTGAGAATAATCATTATGGTATGGGAACTGCAGAGTGGAGAGCAGCCAAGAGTTCAGCCTATTATACGCGTGGTGACTATGTTCCTGGACTGAAGGTGGATGGCATGGATGCCCTTGCTGTCAAGCAAGCTTGTAAGTTTGCCAAGGAGCATGCGTTAAAGAATGGACCCATTATTCTTGAGATGGACACCTACAGGTACCATGGTCATTCTATGTCTGATCCTGGAAGTACCTACCGCACACGTGATGAGATTAGCGGTGTGAGACAGGAACGTGATCCAATTGAAAGGATTAGAAAATTGTTATTATCTCATGAGATAGCCACCGAAAAGGATCTGAAGGATATTGAGAAGGAAATGAGAAAAAAAGTAGATGAAGCCATTGCTCAAGCCAAGGCAAGTCCAATGCCAGATGCGTCAGAACTTTTCACAAACGTGTACGTGAAAGGATTTGAAACTGAGGTGTTTGGAGCAGACCGAAAAGAAGTCAGGGGTGTTCTTCCATAA

Protein sequence

MSHSPISSSSILKPLSSSFRFLSSAAHDSAHLTIQTSVPFTAHKCEPPSRSVDTSPNELLAFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHCTFLGRGGTLLEAFAELMGRQVGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFAQKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSSAYYTRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTYRTRDEISGVRQERDPIERIRKLLLSHEIATEKDLKDIEKEMRKKVDEAIAQAKASPMPDASELFTNVYVKGFETEVFGADRKEVRGVLP
Homology
BLAST of Cmc10g0268181 vs. NCBI nr
Match: XP_008446275.1 (PREDICTED: pyruvate dehydrogenase E1 component subunit alpha, mitochondrial [Cucumis melo] >KAA0034351.1 pyruvate dehydrogenase E1 component subunit alpha [Cucumis melo var. makuwa] >TYK15568.1 pyruvate dehydrogenase E1 component subunit alpha [Cucumis melo var. makuwa])

HSP 1 Score: 776.2 bits (2003), Expect = 1.4e-220
Identity = 388/389 (99.74%), Postives = 388/389 (99.74%), Query Frame = 0

Query: 1   MSHSPISSSSILKPLSSSFRFLSSAAHDSAHLTIQTSVPFTAHKCEPPSRSVDTSPNELL 60
           MSHSPISSSSILKPLSSSFRFLSSAAHDSAHLTIQTSVPFTAHKCEPPSRSVDTSPNELL
Sbjct: 1   MSHSPISSSSILKPLSSSFRFLSSAAHDSAHLTIQTSVPFTAHKCEPPSRSVDTSPNELL 60

Query: 61  AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC 120
           AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC
Sbjct: 61  AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC 120

Query: 121 TFLGRGGTLLEAFAELMGRQVGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA 180
           TFLGRGGTLLEAFAELMGRQVGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA
Sbjct: 121 TFLGRGGTLLEAFAELMGRQVGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA 180

Query: 181 QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSS 240
           QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSS
Sbjct: 181 QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSS 240

Query: 241 AYYTRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY 300
           AYY RGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY
Sbjct: 241 AYYKRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY 300

Query: 301 RTRDEISGVRQERDPIERIRKLLLSHEIATEKDLKDIEKEMRKKVDEAIAQAKASPMPDA 360
           RTRDEISGVRQERDPIERIRKLLLSHEIATEKDLKDIEKEMRKKVDEAIAQAKASPMPDA
Sbjct: 301 RTRDEISGVRQERDPIERIRKLLLSHEIATEKDLKDIEKEMRKKVDEAIAQAKASPMPDA 360

Query: 361 SELFTNVYVKGFETEVFGADRKEVRGVLP 390
           SELFTNVYVKGFETEVFGADRKEVRGVLP
Sbjct: 361 SELFTNVYVKGFETEVFGADRKEVRGVLP 389

BLAST of Cmc10g0268181 vs. NCBI nr
Match: XP_004135218.1 (pyruvate dehydrogenase E1 component subunit alpha, mitochondrial [Cucumis sativus] >KGN51850.1 hypothetical protein Csa_009017 [Cucumis sativus])

HSP 1 Score: 741.5 bits (1913), Expect = 3.7e-210
Identity = 371/389 (95.37%), Postives = 379/389 (97.43%), Query Frame = 0

Query: 1   MSHSPISSSSILKPLSSSFRFLSSAAHDSAHLTIQTSVPFTAHKCEPPSRSVDTSPNELL 60
           MSHS ISSSSILKPLSSSFRFLSSA    +HLTIQTSVPFTAHKCEPPSRSVDTSPNELL
Sbjct: 1   MSHSRISSSSILKPLSSSFRFLSSA----SHLTIQTSVPFTAHKCEPPSRSVDTSPNELL 60

Query: 61  AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC 120
           AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC
Sbjct: 61  AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC 120

Query: 121 TFLGRGGTLLEAFAELMGRQVGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA 180
            FLGRGGTLL++FAELMGRQ GCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA
Sbjct: 121 IFLGRGGTLLQSFAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA 180

Query: 181 QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSS 240
           QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKS+
Sbjct: 181 QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSA 240

Query: 241 AYYTRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY 300
           AYY RGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY
Sbjct: 241 AYYKRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY 300

Query: 301 RTRDEISGVRQERDPIERIRKLLLSHEIATEKDLKDIEKEMRKKVDEAIAQAKASPMPDA 360
           RTRDEISGVRQERDPIERIRKLLLSH++ATEKDLKDIEKEMRK+VDEAIAQAKASPMPD 
Sbjct: 301 RTRDEISGVRQERDPIERIRKLLLSHDLATEKDLKDIEKEMRKEVDEAIAQAKASPMPDL 360

Query: 361 SELFTNVYVKGFETEVFGADRKEVRGVLP 390
           SELF+NVYVKGFE EVFGADRKEVRGVLP
Sbjct: 361 SELFSNVYVKGFEAEVFGADRKEVRGVLP 385

BLAST of Cmc10g0268181 vs. NCBI nr
Match: XP_038891336.1 (pyruvate dehydrogenase E1 component subunit alpha, mitochondrial [Benincasa hispida])

HSP 1 Score: 727.2 bits (1876), Expect = 7.2e-206
Identity = 365/389 (93.83%), Postives = 370/389 (95.12%), Query Frame = 0

Query: 1   MSHSPISSSSILKPLSSSFRFLSSAAHDSAHLTIQTSVPFTAHKCEPPSRSVDTSPNELL 60
           MS S I SSS LKP SSSFR LSSAA  SA LTIQTSVPFTAHKCEPPSRSVDTSP ELL
Sbjct: 1   MSLSRIPSSSFLKPFSSSFRLLSSAADVSADLTIQTSVPFTAHKCEPPSRSVDTSPKELL 60

Query: 61  AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC 120
            FFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC
Sbjct: 61  TFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC 120

Query: 121 TFLGRGGTLLEAFAELMGRQVGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA 180
           TFLGRGGTLLEAFAELMGRQ GCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA
Sbjct: 121 TFLGRGGTLLEAFAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA 180

Query: 181 QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSS 240
           QKYSKEGTVTFALYGDGAANQGQLFEALN+SALWDLPVILVCENNHYGMGTAEWRAAKS 
Sbjct: 181 QKYSKEGTVTFALYGDGAANQGQLFEALNMSALWDLPVILVCENNHYGMGTAEWRAAKSP 240

Query: 241 AYYTRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY 300
           AYY RGDYVPGLKVDGMDALAVKQACKFAK+HALKNGPIILEMDTYRYHGHSMSDPGSTY
Sbjct: 241 AYYKRGDYVPGLKVDGMDALAVKQACKFAKDHALKNGPIILEMDTYRYHGHSMSDPGSTY 300

Query: 301 RTRDEISGVRQERDPIERIRKLLLSHEIATEKDLKDIEKEMRKKVDEAIAQAKASPMPDA 360
           RTRDEISGVRQERDPIERIRKLLLSHEIATEKDLKDIEKEMRK+VDEAIAQAK SPMPD 
Sbjct: 301 RTRDEISGVRQERDPIERIRKLLLSHEIATEKDLKDIEKEMRKEVDEAIAQAKGSPMPDP 360

Query: 361 SELFTNVYVKGFETEVFGADRKEVRGVLP 390
           SELF+NVYVKGF  E FGADRKE+RGVLP
Sbjct: 361 SELFSNVYVKGFGAESFGADRKEIRGVLP 389

BLAST of Cmc10g0268181 vs. NCBI nr
Match: XP_022943269.1 (pyruvate dehydrogenase E1 component subunit alpha, mitochondrial-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 695.7 bits (1794), Expect = 2.3e-196
Identity = 345/382 (90.31%), Postives = 357/382 (93.46%), Query Frame = 0

Query: 8   SSSILKPLSSSFRFLSSAAHDSAHLTIQTSVPFTAHKCEPPSRSVDTSPNELLAFFREMA 67
           SSSILKP SSS+   SSAA   + LTI+TSVPFTAHKCE PSRSV TSPNELLAFFR+MA
Sbjct: 8   SSSILKPFSSSYPLFSSAAGHYSDLTIETSVPFTAHKCETPSRSVHTSPNELLAFFRQMA 67

Query: 68  LMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHCTFLGRGG 127
           LMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHCTFL RGG
Sbjct: 68  LMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHCTFLRRGG 127

Query: 128 TLLEAFAELMGRQVGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFAQKYSKEG 187
           TLLEA AELMGRQ GCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFAQKYSK+G
Sbjct: 128 TLLEALAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFAQKYSKDG 187

Query: 188 TVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSSAYYTRGD 247
           TVTF LYGDGAANQGQLFEALNISALWDLPVI VCENNHYGMGTAEWRAAKS AYY RGD
Sbjct: 188 TVTFVLYGDGAANQGQLFEALNISALWDLPVIFVCENNHYGMGTAEWRAAKSPAYYKRGD 247

Query: 248 YVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTYRTRDEIS 307
           YVPG+KVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTYRTRDEI+
Sbjct: 248 YVPGIKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTYRTRDEIT 307

Query: 308 GVRQERDPIERIRKLLLSHEIATEKDLKDIEKEMRKKVDEAIAQAKASPMPDASELFTNV 367
           G+RQERDPI+RIRKLLLSHEIATEKDLKDIEKEMRK+VDEAIAQAK SPMPD +ELF+NV
Sbjct: 308 GIRQERDPIDRIRKLLLSHEIATEKDLKDIEKEMRKEVDEAIAQAKESPMPDPTELFSNV 367

Query: 368 YVKGFETEVFGADRKEVRGVLP 390
           YV GF  E FGADRKEV  VLP
Sbjct: 368 YVNGFGAEAFGADRKEVMAVLP 389

BLAST of Cmc10g0268181 vs. NCBI nr
Match: XP_023548204.1 (pyruvate dehydrogenase E1 component subunit alpha, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 695.7 bits (1794), Expect = 2.3e-196
Identity = 345/382 (90.31%), Postives = 357/382 (93.46%), Query Frame = 0

Query: 8   SSSILKPLSSSFRFLSSAAHDSAHLTIQTSVPFTAHKCEPPSRSVDTSPNELLAFFREMA 67
           SSSILKP SSS+   SSAA   + LTI+TSVPFTAHKCE PSRSV TSPNELLAFFR+MA
Sbjct: 8   SSSILKPFSSSYPLFSSAAAHYSDLTIETSVPFTAHKCETPSRSVHTSPNELLAFFRQMA 67

Query: 68  LMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHCTFLGRGG 127
           LMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHCTFL RGG
Sbjct: 68  LMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHCTFLRRGG 127

Query: 128 TLLEAFAELMGRQVGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFAQKYSKEG 187
           TLLEA AELMGRQ GCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFAQKYSK+G
Sbjct: 128 TLLEALAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFAQKYSKDG 187

Query: 188 TVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSSAYYTRGD 247
           TVTF LYGDGAANQGQLFEALNISALWDLPVI VCENNHYGMGTAEWRAAKS AYY RGD
Sbjct: 188 TVTFVLYGDGAANQGQLFEALNISALWDLPVIFVCENNHYGMGTAEWRAAKSPAYYKRGD 247

Query: 248 YVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTYRTRDEIS 307
           YVPG+KVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTYRTRDEI+
Sbjct: 248 YVPGIKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTYRTRDEIT 307

Query: 308 GVRQERDPIERIRKLLLSHEIATEKDLKDIEKEMRKKVDEAIAQAKASPMPDASELFTNV 367
           G+RQERDPI+RIRKLLLSHEIATEKDLKDIEKEMRK+VDEAIAQAK SPMPD +ELF+NV
Sbjct: 308 GIRQERDPIDRIRKLLLSHEIATEKDLKDIEKEMRKEVDEAIAQAKESPMPDPTELFSNV 367

Query: 368 YVKGFETEVFGADRKEVRGVLP 390
           YV GF  E FGADRKEV  VLP
Sbjct: 368 YVNGFGAEAFGADRKEVMAVLP 389

BLAST of Cmc10g0268181 vs. ExPASy Swiss-Prot
Match: P52902 (Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 647.5 bits (1669), Expect = 9.6e-185
Identity = 319/389 (82.01%), Postives = 347/389 (89.20%), Query Frame = 0

Query: 2   SHSPISSSSILKPLSSSFRFLSSAAHD-SAHLTIQTSVPFTAHKCEPPSRSVDTSPNELL 61
           S S  + S++  P S++F      + D +A LTI+TS+PFTAH C+PPSRSV TSP+ELL
Sbjct: 9   SSSSSNGSNLFNPFSAAFTLNRPISSDTTATLTIETSLPFTAHNCDPPSRSVTTSPSELL 68

Query: 62  AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC 121
           +FFR MALMRRMEIAADSLYKA LIRGFCHLYDGQEAVAVGMEA  TKKD IITAYRDHC
Sbjct: 69  SFFRTMALMRRMEIAADSLYKANLIRGFCHLYDGQEAVAVGMEAGTTKKDCIITAYRDHC 128

Query: 122 TFLGRGGTLLEAFAELMGRQVGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA 181
           TFLGRGGTLL  +AELMGR+ GCSKGKGGSMHFYKKD+GFYGGHGIVGAQVPLGCGLAF 
Sbjct: 129 TFLGRGGTLLRVYAELMGRRDGCSKGKGGSMHFYKKDSGFYGGHGIVGAQVPLGCGLAFG 188

Query: 182 QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSS 241
           QKY K+ +VTFALYGDGAANQGQLFEALNISALWDLP ILVCENNHYGMGTA WR+AKS 
Sbjct: 189 QKYLKDESVTFALYGDGAANQGQLFEALNISALWDLPAILVCENNHYGMGTATWRSAKSP 248

Query: 242 AYYTRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY 301
           AY+ RGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY
Sbjct: 249 AYFKRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY 308

Query: 302 RTRDEISGVRQERDPIERIRKLLLSHEIATEKDLKDIEKEMRKKVDEAIAQAKASPMPDA 361
           RTRDEISGVRQERDPIER+RKLLLSH+IATEK+LKD EKE+RK+VDEAIA+AK SPMPD 
Sbjct: 309 RTRDEISGVRQERDPIERVRKLLLSHDIATEKELKDTEKEVRKEVDEAIAKAKDSPMPDP 368

Query: 362 SELFTNVYVKGFETEVFGADRKEVRGVLP 390
           S+LF+NVYVKG+  E FG DRKEVR  LP
Sbjct: 369 SDLFSNVYVKGYGVEAFGVDRKEVRVTLP 397

BLAST of Cmc10g0268181 vs. ExPASy Swiss-Prot
Match: P52901 (Pyruvate dehydrogenase E1 component subunit alpha-1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=E1 ALPHA PE=1 SV=2)

HSP 1 Score: 639.8 bits (1649), Expect = 2.0e-182
Identity = 317/391 (81.07%), Postives = 351/391 (89.77%), Query Frame = 0

Query: 1   MSHSPISSSS--ILKPLSSSFRFLSSAAHDSAHLTIQTSVPFTAHKCEPPSRSVDTSPNE 60
           M+ S +SS S  I +P S++F  L S   D+  +TI+TS+PFTAH C+PPSRSV++S  E
Sbjct: 1   MALSRLSSRSNIITRPFSAAFSRLIST--DTTPITIETSLPFTAHLCDPPSRSVESSSQE 60

Query: 61  LLAFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRD 120
           LL FFR MALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVA+GMEAAITKKDAIITAYRD
Sbjct: 61  LLDFFRTMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAIGMEAAITKKDAIITAYRD 120

Query: 121 HCTFLGRGGTLLEAFAELMGRQVGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLA 180
           HC FLGRGG+L E F+ELMGRQ GCSKGKGGSMHFYKK++ FYGGHGIVGAQVPLGCG+A
Sbjct: 121 HCIFLGRGGSLHEVFSELMGRQAGCSKGKGGSMHFYKKESSFYGGHGIVGAQVPLGCGIA 180

Query: 181 FAQKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAK 240
           FAQKY+KE  VTFALYGDGAANQGQLFEALNISALWDLP ILVCENNHYGMGTAEWRAAK
Sbjct: 181 FAQKYNKEEAVTFALYGDGAANQGQLFEALNISALWDLPAILVCENNHYGMGTAEWRAAK 240

Query: 241 SSAYYTRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGS 300
           S +YY RGDYVPGLKVDGMDA AVKQACKFAK+HAL+ GPIILEMDTYRYHGHSMSDPGS
Sbjct: 241 SPSYYKRGDYVPGLKVDGMDAFAVKQACKFAKQHALEKGPIILEMDTYRYHGHSMSDPGS 300

Query: 301 TYRTRDEISGVRQERDPIERIRKLLLSHEIATEKDLKDIEKEMRKKVDEAIAQAKASPMP 360
           TYRTRDEISGVRQERDPIERI+KL+LSH++ATEK+LKD+EKE+RK+VD+AIA+AK  PMP
Sbjct: 301 TYRTRDEISGVRQERDPIERIKKLVLSHDLATEKELKDMEKEIRKEVDDAIAKAKDCPMP 360

Query: 361 DASELFTNVYVKGFETEVFGADRKEVRGVLP 390
           + SELFTNVYVKGF TE FG DRKEV+  LP
Sbjct: 361 EPSELFTNVYVKGFGTESFGPDRKEVKASLP 389

BLAST of Cmc10g0268181 vs. ExPASy Swiss-Prot
Match: Q8H1Y0 (Pyruvate dehydrogenase E1 component subunit alpha-2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IAR4 PE=1 SV=2)

HSP 1 Score: 634.4 bits (1635), Expect = 8.4e-181
Identity = 312/386 (80.83%), Postives = 348/386 (90.16%), Query Frame = 0

Query: 8   SSSILKP----LSSSFRFLSSAAHDSAHLTIQTSVPFTAHKCEPPSRSVDTSPNELLAFF 67
           S++ LKP    L SS R     + DS+ +TI+T+VPFT+H CE PSRSV+TS  E+LAFF
Sbjct: 10  SNTFLKPAITALPSSIR--RHVSTDSSPITIETAVPFTSHLCESPSRSVETSSEEILAFF 69

Query: 68  REMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHCTFL 127
           R+MA MRRMEIAADSLYKAKLIRGFCHLYDGQEA+AVGMEAAITKKDAIIT+YRDHCTF+
Sbjct: 70  RDMARMRRMEIAADSLYKAKLIRGFCHLYDGQEALAVGMEAAITKKDAIITSYRDHCTFI 129

Query: 128 GRGGTLLEAFAELMGRQVGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFAQKY 187
           GRGG L++AF+ELMGR+ GCS GKGGSMHFYKKDA FYGGHGIVGAQ+PLGCGLAFAQKY
Sbjct: 130 GRGGKLVDAFSELMGRKTGCSHGKGGSMHFYKKDASFYGGHGIVGAQIPLGCGLAFAQKY 189

Query: 188 SKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSSAYY 247
           +K+  VTFALYGDGAANQGQLFEALNISALWDLP ILVCENNHYGMGTA WR+AKS AY+
Sbjct: 190 NKDEAVTFALYGDGAANQGQLFEALNISALWDLPAILVCENNHYGMGTATWRSAKSPAYF 249

Query: 248 TRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTYRTR 307
            RGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTYRTR
Sbjct: 250 KRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTYRTR 309

Query: 308 DEISGVRQERDPIERIRKLLLSHEIATEKDLKDIEKEMRKKVDEAIAQAKASPMPDASEL 367
           DEISGVRQ RDPIER+RKLLL+H+IATEK+LKD+EKE+RK+VD+A+AQAK SP+PDASEL
Sbjct: 310 DEISGVRQVRDPIERVRKLLLTHDIATEKELKDMEKEIRKEVDDAVAQAKESPIPDASEL 369

Query: 368 FTNVYVKGFETEVFGADRKEVRGVLP 390
           FTN+YVK    E FGADRKE++  LP
Sbjct: 370 FTNMYVKDCGVESFGADRKELKVTLP 393

BLAST of Cmc10g0268181 vs. ExPASy Swiss-Prot
Match: P52903 (Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial OS=Solanum tuberosum OX=4113 PE=1 SV=1)

HSP 1 Score: 632.1 bits (1629), Expect = 4.2e-180
Identity = 308/380 (81.05%), Postives = 339/380 (89.21%), Query Frame = 0

Query: 11  ILKPLSSSFRFLSSAAHDS-AHLTIQTSVPFTAHKCEPPSRSVDTSPNELLAFFREMALM 70
           I+KPLS++       + DS A +T++TS+PFT+H  +PPSRSV+TSP EL+ FF++M  M
Sbjct: 12  IMKPLSAAVCATRRLSSDSTATITVETSLPFTSHNIDPPSRSVETSPKELMTFFKDMTEM 71

Query: 71  RRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHCTFLGRGGTL 130
           RRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKD IITAYRDHC FLGRGGTL
Sbjct: 72  RRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDCIITAYRDHCIFLGRGGTL 131

Query: 131 LEAFAELMGRQVGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFAQKYSKEGTV 190
           +EAFAELMGR+ GCS+GKGGSMHFYKK++GFYGGHGIVGAQVPLG GLAFAQKY KE  V
Sbjct: 132 VEAFAELMGRRDGCSRGKGGSMHFYKKESGFYGGHGIVGAQVPLGIGLAFAQKYKKEDYV 191

Query: 191 TFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSSAYYTRGDYV 250
           TFA+YGDGAANQGQLFEALN++ALWDLP ILVCENNHYGMGTAEWRAAKS AYY RGDYV
Sbjct: 192 TFAMYGDGAANQGQLFEALNMAALWDLPAILVCENNHYGMGTAEWRAAKSPAYYKRGDYV 251

Query: 251 PGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTYRTRDEISGV 310
           PGL+VDGMD  AVKQAC FAK+HALKNGPIILEMDTYRYHGHSMSDPGSTYRTRDEISGV
Sbjct: 252 PGLRVDGMDVFAVKQACTFAKQHALKNGPIILEMDTYRYHGHSMSDPGSTYRTRDEISGV 311

Query: 311 RQERDPIERIRKLLLSHEIATEKDLKDIEKEMRKKVDEAIAQAKASPMPDASELFTNVYV 370
           RQERDP+ERIR L+L+H IATE +LKDIEKE RK VDEAIA+AK SPMPD SELFTNVYV
Sbjct: 312 RQERDPVERIRSLILAHNIATEAELKDIEKENRKVVDEAIAKAKESPMPDPSELFTNVYV 371

Query: 371 KGFETEVFGADRKEVRGVLP 390
           KGF  E +GADRKE+R  LP
Sbjct: 372 KGFGVEAYGADRKELRATLP 391

BLAST of Cmc10g0268181 vs. ExPASy Swiss-Prot
Match: Q6Z5N4 (Pyruvate dehydrogenase E1 component subunit alpha-1, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0739600 PE=2 SV=1)

HSP 1 Score: 631.7 bits (1628), Expect = 5.4e-180
Identity = 304/366 (83.06%), Postives = 332/366 (90.71%), Query Frame = 0

Query: 24  SAAHDSAHLTIQTSVPFTAHKCEPPSRSVDTSPNELLAFFREMALMRRMEIAADSLYKAK 83
           S +  +A LTI+TSVPFT+H  +PPSR V T+P ELL FFR+M++MRRMEIAADSLYKAK
Sbjct: 25  SISDSTAPLTIETSVPFTSHIVDPPSRDVTTTPAELLTFFRDMSVMRRMEIAADSLYKAK 84

Query: 84  LIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHCTFLGRGGTLLEAFAELMGRQVGC 143
           LIRGFCHLYDGQEAVAVGMEAAIT+ D+IITAYRDHCT+L RGG L+ AFAELMGRQ GC
Sbjct: 85  LIRGFCHLYDGQEAVAVGMEAAITRSDSIITAYRDHCTYLARGGDLVSAFAELMGRQAGC 144

Query: 144 SKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFAQKYSKEGTVTFALYGDGAANQGQ 203
           S+GKGGSMHFYKKDA FYGGHGIVGAQVPLGCGLAFAQKY KE T TFALYGDGAANQGQ
Sbjct: 145 SRGKGGSMHFYKKDANFYGGHGIVGAQVPLGCGLAFAQKYRKEETATFALYGDGAANQGQ 204

Query: 204 LFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSSAYYTRGDYVPGLKVDGMDALAVK 263
           LFEALNISALW LP ILVCENNHYGMGTAEWRAAKS AYY RGDYVPGLKVDGMD LAVK
Sbjct: 205 LFEALNISALWKLPAILVCENNHYGMGTAEWRAAKSPAYYKRGDYVPGLKVDGMDVLAVK 264

Query: 264 QACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTYRTRDEISGVRQERDPIERIRKLL 323
           QACKFAKEHA+ NGPI+LEMDTYRYHGHSMSDPGSTYRTRDEISGVRQERDPIER+RKL+
Sbjct: 265 QACKFAKEHAIANGPIVLEMDTYRYHGHSMSDPGSTYRTRDEISGVRQERDPIERVRKLI 324

Query: 324 LSHEIATEKDLKDIEKEMRKKVDEAIAQAKASPMPDASELFTNVYVKGFETEVFGADRKE 383
           L+H++AT  +LKD+EKE+RK+VD+AIA+AK SPMPD SELFTNVYVKGF  E FGADRKE
Sbjct: 325 LAHDLATAAELKDMEKEIRKEVDDAIAKAKESPMPDTSELFTNVYVKGFGVESFGADRKE 384

Query: 384 VRGVLP 390
           +R  LP
Sbjct: 385 LRATLP 390

BLAST of Cmc10g0268181 vs. ExPASy TrEMBL
Match: A0A5D3CWE3 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00620 PE=4 SV=1)

HSP 1 Score: 776.2 bits (2003), Expect = 6.6e-221
Identity = 388/389 (99.74%), Postives = 388/389 (99.74%), Query Frame = 0

Query: 1   MSHSPISSSSILKPLSSSFRFLSSAAHDSAHLTIQTSVPFTAHKCEPPSRSVDTSPNELL 60
           MSHSPISSSSILKPLSSSFRFLSSAAHDSAHLTIQTSVPFTAHKCEPPSRSVDTSPNELL
Sbjct: 1   MSHSPISSSSILKPLSSSFRFLSSAAHDSAHLTIQTSVPFTAHKCEPPSRSVDTSPNELL 60

Query: 61  AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC 120
           AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC
Sbjct: 61  AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC 120

Query: 121 TFLGRGGTLLEAFAELMGRQVGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA 180
           TFLGRGGTLLEAFAELMGRQVGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA
Sbjct: 121 TFLGRGGTLLEAFAELMGRQVGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA 180

Query: 181 QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSS 240
           QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSS
Sbjct: 181 QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSS 240

Query: 241 AYYTRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY 300
           AYY RGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY
Sbjct: 241 AYYKRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY 300

Query: 301 RTRDEISGVRQERDPIERIRKLLLSHEIATEKDLKDIEKEMRKKVDEAIAQAKASPMPDA 360
           RTRDEISGVRQERDPIERIRKLLLSHEIATEKDLKDIEKEMRKKVDEAIAQAKASPMPDA
Sbjct: 301 RTRDEISGVRQERDPIERIRKLLLSHEIATEKDLKDIEKEMRKKVDEAIAQAKASPMPDA 360

Query: 361 SELFTNVYVKGFETEVFGADRKEVRGVLP 390
           SELFTNVYVKGFETEVFGADRKEVRGVLP
Sbjct: 361 SELFTNVYVKGFETEVFGADRKEVRGVLP 389

BLAST of Cmc10g0268181 vs. ExPASy TrEMBL
Match: A0A1S3BE63 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis melo OX=3656 GN=LOC103489051 PE=4 SV=1)

HSP 1 Score: 776.2 bits (2003), Expect = 6.6e-221
Identity = 388/389 (99.74%), Postives = 388/389 (99.74%), Query Frame = 0

Query: 1   MSHSPISSSSILKPLSSSFRFLSSAAHDSAHLTIQTSVPFTAHKCEPPSRSVDTSPNELL 60
           MSHSPISSSSILKPLSSSFRFLSSAAHDSAHLTIQTSVPFTAHKCEPPSRSVDTSPNELL
Sbjct: 1   MSHSPISSSSILKPLSSSFRFLSSAAHDSAHLTIQTSVPFTAHKCEPPSRSVDTSPNELL 60

Query: 61  AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC 120
           AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC
Sbjct: 61  AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC 120

Query: 121 TFLGRGGTLLEAFAELMGRQVGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA 180
           TFLGRGGTLLEAFAELMGRQVGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA
Sbjct: 121 TFLGRGGTLLEAFAELMGRQVGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA 180

Query: 181 QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSS 240
           QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSS
Sbjct: 181 QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSS 240

Query: 241 AYYTRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY 300
           AYY RGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY
Sbjct: 241 AYYKRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY 300

Query: 301 RTRDEISGVRQERDPIERIRKLLLSHEIATEKDLKDIEKEMRKKVDEAIAQAKASPMPDA 360
           RTRDEISGVRQERDPIERIRKLLLSHEIATEKDLKDIEKEMRKKVDEAIAQAKASPMPDA
Sbjct: 301 RTRDEISGVRQERDPIERIRKLLLSHEIATEKDLKDIEKEMRKKVDEAIAQAKASPMPDA 360

Query: 361 SELFTNVYVKGFETEVFGADRKEVRGVLP 390
           SELFTNVYVKGFETEVFGADRKEVRGVLP
Sbjct: 361 SELFTNVYVKGFETEVFGADRKEVRGVLP 389

BLAST of Cmc10g0268181 vs. ExPASy TrEMBL
Match: A0A0A0KQD6 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis sativus OX=3659 GN=Csa_5G603950 PE=4 SV=1)

HSP 1 Score: 741.5 bits (1913), Expect = 1.8e-210
Identity = 371/389 (95.37%), Postives = 379/389 (97.43%), Query Frame = 0

Query: 1   MSHSPISSSSILKPLSSSFRFLSSAAHDSAHLTIQTSVPFTAHKCEPPSRSVDTSPNELL 60
           MSHS ISSSSILKPLSSSFRFLSSA    +HLTIQTSVPFTAHKCEPPSRSVDTSPNELL
Sbjct: 1   MSHSRISSSSILKPLSSSFRFLSSA----SHLTIQTSVPFTAHKCEPPSRSVDTSPNELL 60

Query: 61  AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC 120
           AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC
Sbjct: 61  AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC 120

Query: 121 TFLGRGGTLLEAFAELMGRQVGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA 180
            FLGRGGTLL++FAELMGRQ GCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA
Sbjct: 121 IFLGRGGTLLQSFAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA 180

Query: 181 QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSS 240
           QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKS+
Sbjct: 181 QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSA 240

Query: 241 AYYTRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY 300
           AYY RGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY
Sbjct: 241 AYYKRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY 300

Query: 301 RTRDEISGVRQERDPIERIRKLLLSHEIATEKDLKDIEKEMRKKVDEAIAQAKASPMPDA 360
           RTRDEISGVRQERDPIERIRKLLLSH++ATEKDLKDIEKEMRK+VDEAIAQAKASPMPD 
Sbjct: 301 RTRDEISGVRQERDPIERIRKLLLSHDLATEKDLKDIEKEMRKEVDEAIAQAKASPMPDL 360

Query: 361 SELFTNVYVKGFETEVFGADRKEVRGVLP 390
           SELF+NVYVKGFE EVFGADRKEVRGVLP
Sbjct: 361 SELFSNVYVKGFEAEVFGADRKEVRGVLP 385

BLAST of Cmc10g0268181 vs. ExPASy TrEMBL
Match: A0A6J1FTT3 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucurbita moschata OX=3662 GN=LOC111448051 PE=4 SV=1)

HSP 1 Score: 695.7 bits (1794), Expect = 1.1e-196
Identity = 345/382 (90.31%), Postives = 357/382 (93.46%), Query Frame = 0

Query: 8   SSSILKPLSSSFRFLSSAAHDSAHLTIQTSVPFTAHKCEPPSRSVDTSPNELLAFFREMA 67
           SSSILKP SSS+   SSAA   + LTI+TSVPFTAHKCE PSRSV TSPNELLAFFR+MA
Sbjct: 8   SSSILKPFSSSYPLFSSAAGHYSDLTIETSVPFTAHKCETPSRSVHTSPNELLAFFRQMA 67

Query: 68  LMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHCTFLGRGG 127
           LMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHCTFL RGG
Sbjct: 68  LMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHCTFLRRGG 127

Query: 128 TLLEAFAELMGRQVGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFAQKYSKEG 187
           TLLEA AELMGRQ GCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFAQKYSK+G
Sbjct: 128 TLLEALAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFAQKYSKDG 187

Query: 188 TVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSSAYYTRGD 247
           TVTF LYGDGAANQGQLFEALNISALWDLPVI VCENNHYGMGTAEWRAAKS AYY RGD
Sbjct: 188 TVTFVLYGDGAANQGQLFEALNISALWDLPVIFVCENNHYGMGTAEWRAAKSPAYYKRGD 247

Query: 248 YVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTYRTRDEIS 307
           YVPG+KVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTYRTRDEI+
Sbjct: 248 YVPGIKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTYRTRDEIT 307

Query: 308 GVRQERDPIERIRKLLLSHEIATEKDLKDIEKEMRKKVDEAIAQAKASPMPDASELFTNV 367
           G+RQERDPI+RIRKLLLSHEIATEKDLKDIEKEMRK+VDEAIAQAK SPMPD +ELF+NV
Sbjct: 308 GIRQERDPIDRIRKLLLSHEIATEKDLKDIEKEMRKEVDEAIAQAKESPMPDPTELFSNV 367

Query: 368 YVKGFETEVFGADRKEVRGVLP 390
           YV GF  E FGADRKEV  VLP
Sbjct: 368 YVNGFGAEAFGADRKEVMAVLP 389

BLAST of Cmc10g0268181 vs. ExPASy TrEMBL
Match: A0A5D3BMT4 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold360G00770 PE=4 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 9.9e-193
Identity = 339/396 (85.61%), Postives = 365/396 (92.17%), Query Frame = 0

Query: 1   MSHSPISSSSILKPLSSSFRF-------LSSAAHDSAHLTIQTSVPFTAHKCEPPSRSVD 60
           +SH+  S S++LKPL+S F F        SSA+ DSA LT++TSVPFTAH C+ PSRSV 
Sbjct: 3   LSHA-ASRSNLLKPLASGFSFSSSFRRSFSSASDDSATLTVETSVPFTAHNCDEPSRSVQ 62

Query: 61  TSPNELLAFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAII 120
           TSP ELL FFR+MALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAII
Sbjct: 63  TSPKELLGFFRDMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAII 122

Query: 121 TAYRDHCTFLGRGGTLLEAFAELMGRQVGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPL 180
           TAYRDHCTFLGRGGTLLE FAELMGRQ GCS+GKGGSMHFYKKDA FYGGHGIVGAQVPL
Sbjct: 123 TAYRDHCTFLGRGGTLLEVFAELMGRQAGCSRGKGGSMHFYKKDAAFYGGHGIVGAQVPL 182

Query: 181 GCGLAFAQKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAE 240
           GCG+AFAQKYSK+ TVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAE
Sbjct: 183 GCGVAFAQKYSKDETVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAE 242

Query: 241 WRAAKSSAYYTRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSM 300
           WRAAKS AYY RGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSM
Sbjct: 243 WRAAKSPAYYKRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSM 302

Query: 301 SDPGSTYRTRDEISGVRQERDPIERIRKLLLSHEIATEKDLKDIEKEMRKKVDEAIAQAK 360
           SDPGSTYRTRDEISGVRQERDPIERIRKL+LS+++ATEK+LKD+EKE+R++VDEAIAQAK
Sbjct: 303 SDPGSTYRTRDEISGVRQERDPIERIRKLVLSYDLATEKELKDVEKEVRREVDEAIAQAK 362

Query: 361 ASPMPDASELFTNVYVKGFETEVFGADRKEVRGVLP 390
            +P+PD +ELFTNVYVKGF TE +GADRKEVR VLP
Sbjct: 363 ENPLPDPAELFTNVYVKGFGTESYGADRKEVRAVLP 397

BLAST of Cmc10g0268181 vs. TAIR 10
Match: AT1G59900.1 (pyruvate dehydrogenase complex E1 alpha subunit )

HSP 1 Score: 639.8 bits (1649), Expect = 1.4e-183
Identity = 317/391 (81.07%), Postives = 351/391 (89.77%), Query Frame = 0

Query: 1   MSHSPISSSS--ILKPLSSSFRFLSSAAHDSAHLTIQTSVPFTAHKCEPPSRSVDTSPNE 60
           M+ S +SS S  I +P S++F  L S   D+  +TI+TS+PFTAH C+PPSRSV++S  E
Sbjct: 1   MALSRLSSRSNIITRPFSAAFSRLIST--DTTPITIETSLPFTAHLCDPPSRSVESSSQE 60

Query: 61  LLAFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRD 120
           LL FFR MALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVA+GMEAAITKKDAIITAYRD
Sbjct: 61  LLDFFRTMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAIGMEAAITKKDAIITAYRD 120

Query: 121 HCTFLGRGGTLLEAFAELMGRQVGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLA 180
           HC FLGRGG+L E F+ELMGRQ GCSKGKGGSMHFYKK++ FYGGHGIVGAQVPLGCG+A
Sbjct: 121 HCIFLGRGGSLHEVFSELMGRQAGCSKGKGGSMHFYKKESSFYGGHGIVGAQVPLGCGIA 180

Query: 181 FAQKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAK 240
           FAQKY+KE  VTFALYGDGAANQGQLFEALNISALWDLP ILVCENNHYGMGTAEWRAAK
Sbjct: 181 FAQKYNKEEAVTFALYGDGAANQGQLFEALNISALWDLPAILVCENNHYGMGTAEWRAAK 240

Query: 241 SSAYYTRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGS 300
           S +YY RGDYVPGLKVDGMDA AVKQACKFAK+HAL+ GPIILEMDTYRYHGHSMSDPGS
Sbjct: 241 SPSYYKRGDYVPGLKVDGMDAFAVKQACKFAKQHALEKGPIILEMDTYRYHGHSMSDPGS 300

Query: 301 TYRTRDEISGVRQERDPIERIRKLLLSHEIATEKDLKDIEKEMRKKVDEAIAQAKASPMP 360
           TYRTRDEISGVRQERDPIERI+KL+LSH++ATEK+LKD+EKE+RK+VD+AIA+AK  PMP
Sbjct: 301 TYRTRDEISGVRQERDPIERIKKLVLSHDLATEKELKDMEKEIRKEVDDAIAKAKDCPMP 360

Query: 361 DASELFTNVYVKGFETEVFGADRKEVRGVLP 390
           + SELFTNVYVKGF TE FG DRKEV+  LP
Sbjct: 361 EPSELFTNVYVKGFGTESFGPDRKEVKASLP 389

BLAST of Cmc10g0268181 vs. TAIR 10
Match: AT1G24180.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 634.4 bits (1635), Expect = 5.9e-182
Identity = 312/386 (80.83%), Postives = 348/386 (90.16%), Query Frame = 0

Query: 8   SSSILKP----LSSSFRFLSSAAHDSAHLTIQTSVPFTAHKCEPPSRSVDTSPNELLAFF 67
           S++ LKP    L SS R     + DS+ +TI+T+VPFT+H CE PSRSV+TS  E+LAFF
Sbjct: 10  SNTFLKPAITALPSSIR--RHVSTDSSPITIETAVPFTSHLCESPSRSVETSSEEILAFF 69

Query: 68  REMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHCTFL 127
           R+MA MRRMEIAADSLYKAKLIRGFCHLYDGQEA+AVGMEAAITKKDAIIT+YRDHCTF+
Sbjct: 70  RDMARMRRMEIAADSLYKAKLIRGFCHLYDGQEALAVGMEAAITKKDAIITSYRDHCTFI 129

Query: 128 GRGGTLLEAFAELMGRQVGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFAQKY 187
           GRGG L++AF+ELMGR+ GCS GKGGSMHFYKKDA FYGGHGIVGAQ+PLGCGLAFAQKY
Sbjct: 130 GRGGKLVDAFSELMGRKTGCSHGKGGSMHFYKKDASFYGGHGIVGAQIPLGCGLAFAQKY 189

Query: 188 SKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSSAYY 247
           +K+  VTFALYGDGAANQGQLFEALNISALWDLP ILVCENNHYGMGTA WR+AKS AY+
Sbjct: 190 NKDEAVTFALYGDGAANQGQLFEALNISALWDLPAILVCENNHYGMGTATWRSAKSPAYF 249

Query: 248 TRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTYRTR 307
            RGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTYRTR
Sbjct: 250 KRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTYRTR 309

Query: 308 DEISGVRQERDPIERIRKLLLSHEIATEKDLKDIEKEMRKKVDEAIAQAKASPMPDASEL 367
           DEISGVRQ RDPIER+RKLLL+H+IATEK+LKD+EKE+RK+VD+A+AQAK SP+PDASEL
Sbjct: 310 DEISGVRQVRDPIERVRKLLLTHDIATEKELKDMEKEIRKEVDDAVAQAKESPIPDASEL 369

Query: 368 FTNVYVKGFETEVFGADRKEVRGVLP 390
           FTN+YVK    E FGADRKE++  LP
Sbjct: 370 FTNMYVKDCGVESFGADRKELKVTLP 393

BLAST of Cmc10g0268181 vs. TAIR 10
Match: AT1G01090.1 (pyruvate dehydrogenase E1 alpha )

HSP 1 Score: 235.3 bits (599), Expect = 8.0e-62
Identity = 137/363 (37.74%), Postives = 205/363 (56.47%), Query Frame = 0

Query: 23  SSAAHDSAHLTIQTSVPFTAHKCEPPSRSVDTSPNELLAFFREMALMRRME-IAADSLYK 82
           S+A   S  +++Q  V     K    + S+  +  E L  + +M L R  E + A   Y+
Sbjct: 51  SNATRRSPVVSVQEVV---KEKQSTNNTSLLITKEEGLELYEDMILGRSFEDMCAQMYYR 110

Query: 83  AKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHCTFLGRGGTLLEAFAELMGRQV 142
            K+  GF HLY+GQEAV+ G    +TK D++++ YRDH   L +G +     +EL G+  
Sbjct: 111 GKMF-GFVHLYNGQEAVSTGFIKLLTKSDSVVSTYRDHVHALSKGVSARAVMSELFGKVT 170

Query: 143 GCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFAQKYSKE------GTVTFALYG 202
           GC +G+GGSMH + K+    GG   +G  +P+  G AF+ KY +E        VT A +G
Sbjct: 171 GCCRGQGGSMHMFSKEHNMLGGFAFIGEGIPVATGAAFSSKYRREVLKQDCDDVTVAFFG 230

Query: 203 DGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSSAYYTRGDY--VPGLK 262
           DG  N GQ FE LN++AL+ LP+I V ENN + +G +  RA      + +G    +PG+ 
Sbjct: 231 DGTCNNGQFFECLNMAALYKLPIIFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVH 290

Query: 263 VDGMDALAVKQACKFAKEHALK-NGPIILEMDTYRYHGHSMSDPGSTYRTRDEISGVR-Q 322
           VDGMD L V++  K A   A +  GP ++E +TYR+ GHS++DP      RD     +  
Sbjct: 291 VDGMDVLKVREVAKEAVTRARRGEGPTLVECETYRFRGHSLADPD---ELRDAAEKAKYA 350

Query: 323 ERDPIERIRKLLLSHEIATEKDLKDIEKEMRKKVDEAIAQAKASPMPDASELFTNVYV-- 373
            RDPI  ++K L+ +++A E +LK IEK++ + V+EA+  A ASP P  S+L  NV+   
Sbjct: 351 ARDPIAALKKYLIENKLAKEAELKSIEKKIDELVEEAVEFADASPQPGRSQLLENVFADP 406

BLAST of Cmc10g0268181 vs. TAIR 10
Match: AT1G21400.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 147.9 bits (372), Expect = 1.7e-35
Identity = 92/328 (28.05%), Postives = 158/328 (48.17%), Query Frame = 0

Query: 47  PPSRSVDTSPNELLAFFREMALMRRMEIAADSLYKAKLIRGFCHLY---DGQEAVAVGME 106
           P S  +  S    +  + +MA ++ M    D ++     +G    Y    G+EA+ +   
Sbjct: 118 PDSDFIPVSEKLAVRMYEQMATLQVM----DHIFYEAQRQGRISFYLTSVGEEAINIASA 177

Query: 107 AAITKKDAIITAYRDHCTFLGRGGTLLEAFAELMGRQVGCSKGKGGSMHFYKKDAGFYGG 166
           AA++  D ++  YR+    L RG TL E   +  G +    KG+   +H+      ++  
Sbjct: 178 AALSPDDVVLPQYREPGVLLWRGFTLEEFANQCFGNKADYGKGRQMPIHYGSNRLNYFTI 237

Query: 167 HGIVGAQVPLGCGLAFAQKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCE 226
              +  Q+P   G+ ++ K  K+   T    GDG  ++G     LN +A+ + PV+ +C 
Sbjct: 238 SSPIATQLPQAAGVGYSLKMDKKNACTVTFIGDGGTSEGDFHAGLNFAAVMEAPVVFICR 297

Query: 227 NNHYGMGTAEWRAAKSSAYYTRGDY--VPGLKVDGMDALAVKQACKFAKEHAL-KNGPII 286
           NN + + T      +S     +G    +  ++VDG DALAV  A + A+E A+ +  P++
Sbjct: 298 NNGWAISTHISEQFRSDGIVVKGQAYGIRSIRVDGNDALAVYSAVRSAREMAVTEQRPVL 357

Query: 287 LEMDTYRYHGHSMSDPGSTYRTRDEISGVRQERDPIERIRKLLLSHEIATEKDLKDIEKE 346
           +EM TYR   HS SD  + YR  DEI   +  R+P+ R RK +  +   +E+D   +   
Sbjct: 358 IEMMTYRVGHHSTSDDSTKYRAADEIQYWKMSRNPVNRFRKWVEDNGWWSEEDESKLRSN 417

Query: 347 MRKKVDEAIAQAKASPMPDASELFTNVY 369
            RK++ +AI  A+       +ELF +VY
Sbjct: 418 ARKQLLQAIQAAEKWEKQPLTELFNDVY 441

BLAST of Cmc10g0268181 vs. TAIR 10
Match: AT5G09300.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 146.7 bits (369), Expect = 3.8e-35
Identity = 91/326 (27.91%), Postives = 161/326 (49.39%), Query Frame = 0

Query: 49  SRSVDTSPNELLAFFREMALMRRMEIAADSLYKAKLIRGFCHLYD---GQEAVAVGMEAA 108
           S+ V  S    +  + +M  ++ M    D+++     +G    Y    G+EA+ +   AA
Sbjct: 120 SQFVQVSEEVAVKIYSDMVTLQIM----DNIFYEAQRQGRLSFYATAIGEEAINIASAAA 179

Query: 109 ITKKDAIITAYRDHCTFLGRGGTLLEAFAELMGRQVGCSKGKGGSMHFYKKDAGFYGGHG 168
           +T +D I   YR+    L RG TL E   +  G +    KG+   +H+      ++    
Sbjct: 180 LTPQDVIFPQYREPGVLLWRGFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSA 239

Query: 169 IVGAQVPLGCGLAFAQKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENN 228
            +  Q+P   G A++ K  K+       +GDG  ++G    ALNI+A+ + PV+ +C NN
Sbjct: 240 TIATQLPNAVGAAYSLKMDKKDACAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNN 299

Query: 229 HYGMGTAEWRAAKSSAYYTRGDY--VPGLKVDGMDALAVKQACKFAKEHALK-NGPIILE 288
            + + T      +S     +G    +  ++VDG DALA+  A   A+E A++   PI++E
Sbjct: 300 GWAISTPTSDQFRSDGVVVKGRAYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIE 359

Query: 289 MDTYRYHGHSMSDPGSTYRTRDEISGVRQERDPIERIRKLLLSHEIATEKDLKDIEKEMR 348
             TYR   HS SD  + YR+  EI    + R+P+ R R  + S+   ++K   D+   ++
Sbjct: 360 ALTYRVGHHSTSDDSTRYRSAGEIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIK 419

Query: 349 KKVDEAIAQAKASPMPDASELFTNVY 369
           K++ EA+  A+ +  P+   +F++VY
Sbjct: 420 KEMLEALRVAEKTEKPNLQNMFSDVY 441

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008446275.11.4e-22099.74PREDICTED: pyruvate dehydrogenase E1 component subunit alpha, mitochondrial [Cuc... [more]
XP_004135218.13.7e-21095.37pyruvate dehydrogenase E1 component subunit alpha, mitochondrial [Cucumis sativu... [more]
XP_038891336.17.2e-20693.83pyruvate dehydrogenase E1 component subunit alpha, mitochondrial [Benincasa hisp... [more]
XP_022943269.12.3e-19690.31pyruvate dehydrogenase E1 component subunit alpha, mitochondrial-like isoform X2... [more]
XP_023548204.12.3e-19690.31pyruvate dehydrogenase E1 component subunit alpha, mitochondrial-like [Cucurbita... [more]
Match NameE-valueIdentityDescription
P529029.6e-18582.01Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial OS=Pisum sativu... [more]
P529012.0e-18281.07Pyruvate dehydrogenase E1 component subunit alpha-1, mitochondrial OS=Arabidopsi... [more]
Q8H1Y08.4e-18180.83Pyruvate dehydrogenase E1 component subunit alpha-2, mitochondrial OS=Arabidopsi... [more]
P529034.2e-18081.05Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial OS=Solanum tube... [more]
Q6Z5N45.4e-18083.06Pyruvate dehydrogenase E1 component subunit alpha-1, mitochondrial OS=Oryza sati... [more]
Match NameE-valueIdentityDescription
A0A5D3CWE36.6e-22199.74Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis melo var. makuwa OX... [more]
A0A1S3BE636.6e-22199.74Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A0A0KQD61.8e-21095.37Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis sativus OX=3659 GN=... [more]
A0A6J1FTT31.1e-19690.31Pyruvate dehydrogenase E1 component subunit alpha OS=Cucurbita moschata OX=3662 ... [more]
A0A5D3BMT49.9e-19385.61Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis melo var. makuwa OX... [more]
Match NameE-valueIdentityDescription
AT1G59900.11.4e-18381.07pyruvate dehydrogenase complex E1 alpha subunit [more]
AT1G24180.15.9e-18280.83Thiamin diphosphate-binding fold (THDP-binding) superfamily protein [more]
AT1G01090.18.0e-6237.74pyruvate dehydrogenase E1 alpha [more]
AT1G21400.11.7e-3528.05Thiamin diphosphate-binding fold (THDP-binding) superfamily protein [more]
AT5G09300.13.8e-3527.91Thiamin diphosphate-binding fold (THDP-binding) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.970coord: 3..384
e-value: 5.1E-132
score: 442.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 292..311
NoneNo IPR availablePANTHERPTHR11516:SF65PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT ALPHA, MITOCHONDRIALcoord: 10..389
NoneNo IPR availablePANTHERPTHR11516PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT BACTERIAL AND ORGANELLARcoord: 10..389
NoneNo IPR availableCDDcd02000TPP_E1_PDC_ADC_BCADCcoord: 62..352
e-value: 4.22516E-141
score: 401.489
IPR001017Dehydrogenase, E1 componentPFAMPF00676E1_dhcoord: 65..360
e-value: 1.1E-117
score: 392.3
IPR017597Pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit, subgroup yTIGRFAMTIGR03182TIGR03182coord: 57..369
e-value: 8.5E-127
score: 420.3
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 48..371

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc10g0268181.1Cmc10g0268181.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006086 acetyl-CoA biosynthetic process from pyruvate
biological_process GO:0006096 glycolytic process
biological_process GO:0046686 response to cadmium ion
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0004739 pyruvate dehydrogenase (acetyl-transferring) activity
molecular_function GO:0016624 oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor