Cmc09g0257011 (gene) Melon (Charmono) v1.1

Overview
NameCmc09g0257011
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Descriptionsubtilisin-like protease SBT1.7
LocationCMiso1.1chr09: 21646595 .. 21648859 (-)
RNA-Seq ExpressionCmc09g0257011
SyntenyCmc09g0257011
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTTTTCTTCCTTTCATTTGTATATTTTTCTTCCTAAACTTTCATGGCTATGTTGCCATGAAAACTGAGCTTTCAAGTACTTCCAACACCAATTTACAAACCTACATTGTCCATGTAAAGCAGCCAGTGCTTGAGATTTTGGGTGACACCATTGATCTCCAAAATTGGTACACATCATTCTTACCAGAAACCATCGAAGCGTCATCCGACGAACAACCACGGTTACTTTATTCATATCGACACGTGATGAGTGGTTTCTCTGCAAGACTTACAAAAGAGCAAGTTAAAGCAATGGAAGAGAAGGATGGTTTCATCTCGGCCATGCCTGAAACCACATTGAATTTGCACACAACTCATACACCTGAATATTTGGGGTTGAACACACATTTTGGGTTATGGAAGAATTCAAACTTTGGTAAAGGAGTGATCATAGGAGTATTGGATACTGGAATTCATCCAAATCATCCTTCGTTTAACGATGAAGGAATGTCGTCACCACCGGCGAAATGGAAAGGGAGATGCGAATTTGGTGCTTCTATTTGTAATAACAAGTTGATTGGTGCAAGAACTTTCAATCTTGCTAATAATTTCGTGATTGGGAAATCACCAAATGATGAAAATGGACATGGCACACACACAGCAAGCACAGCTGCAGGCACTTTCGTTAAAGGTGCTGAAGCTTTGGGAAATGCAAGAGGCAAAGCTGTTGGAATGGCACCTTTAGCTCATATTGCAATGTATAAAGTTTGTTCTCCAAATGGTTGTTCGAGTAGTGATATACTCGCTGCGTTAGATGCTGCAATCGACGACGGTGTTGACGTGCTCTCGCTCTCCCTCGGTGCCCCATCGACTCCATTTTTCAGAGACACTATTGCCATAGGGGCATTTGCAGCCATTAAAAAGGGAATTTTTGTGAGTTGTTCAGCTGGGAATTCAGGCCCTTCCAAAAACACATTAGCCAACGAAGCACCATGGATTCTAACTGTTGGAGCAAGCACTATTGATCGAAAAATCGTGGCATTGGCAAAGCTTGGAAGTGGCGAAGTCTACACCGGTGAATCTCTGTTCCAGCCCAGAAATTTTCCATCAAAATTCTTACCACTTGTATACGCTGGCAAAAGCGGAATCGAAGGGTCTGAATATTGTATTCAAGGTTCCCTTGAAAAGCTGAATGTGACAGGAAAAGTCGTGGTATGTGAACGAGGAGGAGGAATAAGCAGAGTAGCAAAAGGGTTGGTTGTGAAAAATGGTGGTGGAGCTGCCATGATTCTTATAAACCAAAAACCAGAAGGGTTTAGTACTTTAGCTGAAGCTCATGTTCTTCCAACGACTCATTTAAGCTATGAAGATGGACTTAAAATCAAAGCATATATAAATTCATCACATAATCCAAAAGCTTCAATTTCATTTAAAGGAACTCTGCTTGGAAACAGAGCCACCACTTTCTCCCCTGCCATGGCTTCTTTTTCTTCACGAGGGCCATGTCAAGCAAGCCCTGGGATCTTGAAACCTGACATAACGGGCCCTGGTGTCAACATTCTCGCAGCTTGGCCATTCCCATTAAACAACAATACGAACACGAACACAAAATCGACGTTTAATGTTATATCAGGAACATCAATGTCTTGTCCTCATTTAAGTGGAATTGCAGCTTTGATCAAGAGTTATCATCCTAATTGGTCCCCTGCTGCCATTAAATCTGCTATTATGACCTCTGCAGATGTAAGAAATCCTCAAGGGAAACCAATCATGGATCAAGATTTGAAACCAGCAAACTTTTTCGCTATGGGGTCCGGACATGTTAATCCATCAAAAGCAGCTAACCCAGGATTGGTCTATGACATTCAGCCTGATGATTATATTCCTTATCTTTGCCATTTGTACACAGATGCTCAAGTGTCGATTATTGTTCGTAAACAAGTAACGTGTTCGACGGTGCCAAGAATACGAGAAGGGGATTTGAATTATCCATCGTTTGCTGTGAGTTTAGAAGCTGGTTCACAGACGTTTAATAGAACAGTGACGAATGTGGGTGATGCTAATTCAGTTTATTACCCCATTGTTGAGGCACCAGCTGGAGTTTCAGTGAAAGTTACACCAAGCAATTTGAAGTTCTCAAAGTTGAATGAAAAAGTGACATATTCGGTAACCTTCAGCCGGATTGACTTTGTTCGAACAACGAGTGAATTCAGTGAAGGGTATCTCATATGGGTTTCTAAAAAACTCATGGTGAGGAGTCCAATCTCTGTGAAGCTCACCTAA

mRNA sequence

ATGGTTTTTCTTCCTTTCATTTGTATATTTTTCTTCCTAAACTTTCATGGCTATGTTGCCATGAAAACTGAGCTTTCAAGTACTTCCAACACCAATTTACAAACCTACATTGTCCATGTAAAGCAGCCAGTGCTTGAGATTTTGGGTGACACCATTGATCTCCAAAATTGGTACACATCATTCTTACCAGAAACCATCGAAGCGTCATCCGACGAACAACCACGGTTACTTTATTCATATCGACACGTGATGAGTGGTTTCTCTGCAAGACTTACAAAAGAGCAAGTTAAAGCAATGGAAGAGAAGGATGGTTTCATCTCGGCCATGCCTGAAACCACATTGAATTTGCACACAACTCATACACCTGAATATTTGGGGTTGAACACACATTTTGGGTTATGGAAGAATTCAAACTTTGGTAAAGGAGTGATCATAGGAGTATTGGATACTGGAATTCATCCAAATCATCCTTCGTTTAACGATGAAGGAATGTCGTCACCACCGGCGAAATGGAAAGGGAGATGCGAATTTGGTGCTTCTATTTGTAATAACAAGTTGATTGGTGCAAGAACTTTCAATCTTGCTAATAATTTCGTGATTGGGAAATCACCAAATGATGAAAATGGACATGGCACACACACAGCAAGCACAGCTGCAGGCACTTTCGTTAAAGGTGCTGAAGCTTTGGGAAATGCAAGAGGCAAAGCTGTTGGAATGGCACCTTTAGCTCATATTGCAATGTATAAAGTTTGTTCTCCAAATGGTTGTTCGAGTAGTGATATACTCGCTGCGTTAGATGCTGCAATCGACGACGGTGTTGACGTGCTCTCGCTCTCCCTCGGTGCCCCATCGACTCCATTTTTCAGAGACACTATTGCCATAGGGGCATTTGCAGCCATTAAAAAGGGAATTTTTGTGAGTTGTTCAGCTGGGAATTCAGGCCCTTCCAAAAACACATTAGCCAACGAAGCACCATGGATTCTAACTGTTGGAGCAAGCACTATTGATCGAAAAATCGTGGCATTGGCAAAGCTTGGAAGTGGCGAAGTCTACACCGGTGAATCTCTGTTCCAGCCCAGAAATTTTCCATCAAAATTCTTACCACTTGTATACGCTGGCAAAAGCGGAATCGAAGGGTCTGAATATTGTATTCAAGGTTCCCTTGAAAAGCTGAATGTGACAGGAAAAGTCGTGGTATGTGAACGAGGAGGAGGAATAAGCAGAGTAGCAAAAGGGTTGGTTGTGAAAAATGGTGGTGGAGCTGCCATGATTCTTATAAACCAAAAACCAGAAGGGTTTAGTACTTTAGCTGAAGCTCATGTTCTTCCAACGACTCATTTAAGCTATGAAGATGGACTTAAAATCAAAGCATATATAAATTCATCACATAATCCAAAAGCTTCAATTTCATTTAAAGGAACTCTGCTTGGAAACAGAGCCACCACTTTCTCCCCTGCCATGGCTTCTTTTTCTTCACGAGGGCCATGTCAAGCAAGCCCTGGGATCTTGAAACCTGACATAACGGGCCCTGGTGTCAACATTCTCGCAGCTTGGCCATTCCCATTAAACAACAATACGAACACGAACACAAAATCGACGTTTAATGTTATATCAGGAACATCAATGTCTTGTCCTCATTTAAGTGGAATTGCAGCTTTGATCAAGAGTTATCATCCTAATTGGTCCCCTGCTGCCATTAAATCTGCTATTATGACCTCTGCAGATGTAAGAAATCCTCAAGGGAAACCAATCATGGATCAAGATTTGAAACCAGCAAACTTTTTCGCTATGGGGTCCGGACATGTTAATCCATCAAAAGCAGCTAACCCAGGATTGGTCTATGACATTCAGCCTGATGATTATATTCCTTATCTTTGCCATTTGTACACAGATGCTCAAGTGTCGATTATTGTTCGTAAACAAGTAACGTGTTCGACGGTGCCAAGAATACGAGAAGGGGATTTGAATTATCCATCGTTTGCTGTGAGTTTAGAAGCTGGTTCACAGACGTTTAATAGAACAGTGACGAATGTGGGTGATGCTAATTCAGTTTATTACCCCATTGTTGAGGCACCAGCTGGAGTTTCAGTGAAAGTTACACCAAGCAATTTGAAGTTCTCAAAGTTGAATGAAAAAGTGACATATTCGGTAACCTTCAGCCGGATTGACTTTGTTCGAACAACGAGTGAATTCAGTGAAGGGTATCTCATATGGGTTTCTAAAAAACTCATGGTGAGGAGTCCAATCTCTGTGAAGCTCACCTAA

Coding sequence (CDS)

ATGGTTTTTCTTCCTTTCATTTGTATATTTTTCTTCCTAAACTTTCATGGCTATGTTGCCATGAAAACTGAGCTTTCAAGTACTTCCAACACCAATTTACAAACCTACATTGTCCATGTAAAGCAGCCAGTGCTTGAGATTTTGGGTGACACCATTGATCTCCAAAATTGGTACACATCATTCTTACCAGAAACCATCGAAGCGTCATCCGACGAACAACCACGGTTACTTTATTCATATCGACACGTGATGAGTGGTTTCTCTGCAAGACTTACAAAAGAGCAAGTTAAAGCAATGGAAGAGAAGGATGGTTTCATCTCGGCCATGCCTGAAACCACATTGAATTTGCACACAACTCATACACCTGAATATTTGGGGTTGAACACACATTTTGGGTTATGGAAGAATTCAAACTTTGGTAAAGGAGTGATCATAGGAGTATTGGATACTGGAATTCATCCAAATCATCCTTCGTTTAACGATGAAGGAATGTCGTCACCACCGGCGAAATGGAAAGGGAGATGCGAATTTGGTGCTTCTATTTGTAATAACAAGTTGATTGGTGCAAGAACTTTCAATCTTGCTAATAATTTCGTGATTGGGAAATCACCAAATGATGAAAATGGACATGGCACACACACAGCAAGCACAGCTGCAGGCACTTTCGTTAAAGGTGCTGAAGCTTTGGGAAATGCAAGAGGCAAAGCTGTTGGAATGGCACCTTTAGCTCATATTGCAATGTATAAAGTTTGTTCTCCAAATGGTTGTTCGAGTAGTGATATACTCGCTGCGTTAGATGCTGCAATCGACGACGGTGTTGACGTGCTCTCGCTCTCCCTCGGTGCCCCATCGACTCCATTTTTCAGAGACACTATTGCCATAGGGGCATTTGCAGCCATTAAAAAGGGAATTTTTGTGAGTTGTTCAGCTGGGAATTCAGGCCCTTCCAAAAACACATTAGCCAACGAAGCACCATGGATTCTAACTGTTGGAGCAAGCACTATTGATCGAAAAATCGTGGCATTGGCAAAGCTTGGAAGTGGCGAAGTCTACACCGGTGAATCTCTGTTCCAGCCCAGAAATTTTCCATCAAAATTCTTACCACTTGTATACGCTGGCAAAAGCGGAATCGAAGGGTCTGAATATTGTATTCAAGGTTCCCTTGAAAAGCTGAATGTGACAGGAAAAGTCGTGGTATGTGAACGAGGAGGAGGAATAAGCAGAGTAGCAAAAGGGTTGGTTGTGAAAAATGGTGGTGGAGCTGCCATGATTCTTATAAACCAAAAACCAGAAGGGTTTAGTACTTTAGCTGAAGCTCATGTTCTTCCAACGACTCATTTAAGCTATGAAGATGGACTTAAAATCAAAGCATATATAAATTCATCACATAATCCAAAAGCTTCAATTTCATTTAAAGGAACTCTGCTTGGAAACAGAGCCACCACTTTCTCCCCTGCCATGGCTTCTTTTTCTTCACGAGGGCCATGTCAAGCAAGCCCTGGGATCTTGAAACCTGACATAACGGGCCCTGGTGTCAACATTCTCGCAGCTTGGCCATTCCCATTAAACAACAATACGAACACGAACACAAAATCGACGTTTAATGTTATATCAGGAACATCAATGTCTTGTCCTCATTTAAGTGGAATTGCAGCTTTGATCAAGAGTTATCATCCTAATTGGTCCCCTGCTGCCATTAAATCTGCTATTATGACCTCTGCAGATGTAAGAAATCCTCAAGGGAAACCAATCATGGATCAAGATTTGAAACCAGCAAACTTTTTCGCTATGGGGTCCGGACATGTTAATCCATCAAAAGCAGCTAACCCAGGATTGGTCTATGACATTCAGCCTGATGATTATATTCCTTATCTTTGCCATTTGTACACAGATGCTCAAGTGTCGATTATTGTTCGTAAACAAGTAACGTGTTCGACGGTGCCAAGAATACGAGAAGGGGATTTGAATTATCCATCGTTTGCTGTGAGTTTAGAAGCTGGTTCACAGACGTTTAATAGAACAGTGACGAATGTGGGTGATGCTAATTCAGTTTATTACCCCATTGTTGAGGCACCAGCTGGAGTTTCAGTGAAAGTTACACCAAGCAATTTGAAGTTCTCAAAGTTGAATGAAAAAGTGACATATTCGGTAACCTTCAGCCGGATTGACTTTGTTCGAACAACGAGTGAATTCAGTGAAGGGTATCTCATATGGGTTTCTAAAAAACTCATGGTGAGGAGTCCAATCTCTGTGAAGCTCACCTAA

Protein sequence

MVFLPFICIFFFLNFHGYVAMKTELSSTSNTNLQTYIVHVKQPVLEILGDTIDLQNWYTSFLPETIEASSDEQPRLLYSYRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNTHFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNFVIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAIGAFAAIKKGIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEGSEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYHPNWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLEAGSQTFNRTVTNVGDANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIWVSKKLMVRSPISVKLT
Homology
BLAST of Cmc09g0257011 vs. NCBI nr
Match: XP_008437177.1 (PREDICTED: subtilisin-like protease SBT1.7 [Cucumis melo])

HSP 1 Score: 1499.2 bits (3880), Expect = 0.0e+00
Identity = 751/754 (99.60%), Postives = 752/754 (99.73%), Query Frame = 0

Query: 1   MVFLPFICIFFFLNFHGYVAMKTELSSTSNTNLQTYIVHVKQPVLEILGDTIDLQNWYTS 60
           MVFLPFICIFFFLNFHGYVAMKTELSSTSNTNLQTYIVHVKQPVLEILGDTIDLQNWYTS
Sbjct: 1   MVFLPFICIFFFLNFHGYVAMKTELSSTSNTNLQTYIVHVKQPVLEILGDTIDLQNWYTS 60

Query: 61  FLPETIEASSDEQPRLLYSYRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTH 120
           FLPETIEASSDEQPRLLYSYRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTH
Sbjct: 61  FLPETIEASSDEQPRLLYSYRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTH 120

Query: 121 TPEYLGLNTHFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGAS 180
           TPEYLGLN HFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGAS
Sbjct: 121 TPEYLGLNKHFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGAS 180

Query: 181 ICNNKLIGARTFNLANNFVIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMA 240
           ICNNKLIGARTFNLANNFVIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMA
Sbjct: 181 ICNNKLIGARTFNLANNFVIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMA 240

Query: 241 PLAHIAMYKVCSPNGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAIGAFAAI 300
           PLAHIAMYKVCSPNGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIA+GAFAAI
Sbjct: 241 PLAHIAMYKVCSPNGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAMGAFAAI 300

Query: 301 KKGIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPR 360
           KKGIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPR
Sbjct: 301 KKGIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPR 360

Query: 361 NFPSKFLPLVYAGKSGIEGSEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGG 420
           NFPSKFLPLVYAGKSGIEGSEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGG
Sbjct: 361 NFPSKFLPLVYAGKSGIEGSEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGG 420

Query: 421 AAMILINQKPEGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRA 480
           AAMILINQKPEGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRA
Sbjct: 421 AAMILINQKPEGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRA 480

Query: 481 TTFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGT 540
           TTFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGT
Sbjct: 481 TTFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGT 540

Query: 541 SMSCPHLSGIAALIKSYHPNWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSG 600
           SMSCPHLSGIAALIKSYHPNWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSG
Sbjct: 541 SMSCPHLSGIAALIKSYHPNWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSG 600

Query: 601 HVNPSKAANPGLVYDIQPDDYIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSF 660
           HVNPSKAANPGLVYDIQPDDYIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSF
Sbjct: 601 HVNPSKAANPGLVYDIQPDDYIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSF 660

Query: 661 AVSLEAGSQTFNRTVTNVGDANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFS 720
           AVSL AGSQTFNRTVTNVGDANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFS
Sbjct: 661 AVSLGAGSQTFNRTVTNVGDANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFS 720

Query: 721 RIDFVRTTSEFSEGYLIWVSKKLMVRSPISVKLT 755
           RIDFVRTTSEFSEGYLIWVSKKLMVRSPISVKLT
Sbjct: 721 RIDFVRTTSEFSEGYLIWVSKKLMVRSPISVKLT 754

BLAST of Cmc09g0257011 vs. NCBI nr
Match: KAA0042822.1 (subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa])

HSP 1 Score: 1466.8 bits (3796), Expect = 0.0e+00
Identity = 734/734 (100.00%), Postives = 734/734 (100.00%), Query Frame = 0

Query: 21  MKTELSSTSNTNLQTYIVHVKQPVLEILGDTIDLQNWYTSFLPETIEASSDEQPRLLYSY 80
           MKTELSSTSNTNLQTYIVHVKQPVLEILGDTIDLQNWYTSFLPETIEASSDEQPRLLYSY
Sbjct: 1   MKTELSSTSNTNLQTYIVHVKQPVLEILGDTIDLQNWYTSFLPETIEASSDEQPRLLYSY 60

Query: 81  RHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNTHFGLWKNSNFG 140
           RHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNTHFGLWKNSNFG
Sbjct: 61  RHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNTHFGLWKNSNFG 120

Query: 141 KGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNFVI 200
           KGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNFVI
Sbjct: 121 KGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNFVI 180

Query: 201 GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSSD 260
           GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSSD
Sbjct: 181 GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSSD 240

Query: 261 ILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAIGAFAAIKKGIFVSCSAGNSGPSKNTL 320
           ILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAIGAFAAIKKGIFVSCSAGNSGPSKNTL
Sbjct: 241 ILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAIGAFAAIKKGIFVSCSAGNSGPSKNTL 300

Query: 321 ANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEGS 380
           ANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEGS
Sbjct: 301 ANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEGS 360

Query: 381 EYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAEAH 440
           EYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAEAH
Sbjct: 361 EYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAEAH 420

Query: 441 VLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQASP 500
           VLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQASP
Sbjct: 421 VLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQASP 480

Query: 501 GILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYHPN 560
           GILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYHPN
Sbjct: 481 GILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYHPN 540

Query: 561 WSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD 620
           WSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD
Sbjct: 541 WSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD 600

Query: 621 YIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLEAGSQTFNRTVTNVGD 680
           YIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLEAGSQTFNRTVTNVGD
Sbjct: 601 YIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLEAGSQTFNRTVTNVGD 660

Query: 681 ANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIWVS 740
           ANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIWVS
Sbjct: 661 ANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIWVS 720

Query: 741 KKLMVRSPISVKLT 755
           KKLMVRSPISVKLT
Sbjct: 721 KKLMVRSPISVKLT 734

BLAST of Cmc09g0257011 vs. NCBI nr
Match: XP_004147599.2 (subtilisin-like protease SBT1.7 [Cucumis sativus] >KAE8647878.1 hypothetical protein Csa_000520 [Cucumis sativus])

HSP 1 Score: 1420.2 bits (3675), Expect = 0.0e+00
Identity = 707/754 (93.77%), Postives = 730/754 (96.82%), Query Frame = 0

Query: 1   MVFLPFICIFFFLNFHGYVAMKTELSSTSNTNLQTYIVHVKQPVLEILGDTIDLQNWYTS 60
           MVFLPFICIFFFLNFHGYVAMKTEL S SNTNLQTYIVHVKQP +EILGDTIDLQNWYTS
Sbjct: 1   MVFLPFICIFFFLNFHGYVAMKTELWSASNTNLQTYIVHVKQPEVEILGDTIDLQNWYTS 60

Query: 61  FLPETIEASSDEQPRLLYSYRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTH 120
           FLPETIEASS+EQ RLLYSYRHV+SGFSARLTKEQVK MEEKDGFISAMPETTLNLHTTH
Sbjct: 61  FLPETIEASSNEQSRLLYSYRHVISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTH 120

Query: 121 TPEYLGLNTHFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGAS 180
           TPEYLGLN HFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGM SPPAKWKGRCEFGAS
Sbjct: 121 TPEYLGLNQHFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFGAS 180

Query: 181 ICNNKLIGARTFNLANNFVIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMA 240
           ICNNKLIGARTFNLANN  IGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMA
Sbjct: 181 ICNNKLIGARTFNLANNVSIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMA 240

Query: 241 PLAHIAMYKVCSPNGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAIGAFAAI 300
           PLAHIA+YKVCSP GCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFF+DTIA+GAFAAI
Sbjct: 241 PLAHIAVYKVCSPKGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAI 300

Query: 301 KKGIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPR 360
           KKGIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKL SG+V+TGESLFQPR
Sbjct: 301 KKGIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPR 360

Query: 361 NFPSKFLPLVYAGKSGIEGSEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGG 420
           +F SKFLPLVYAGKSGIEGSEYC++GSLEKLNVTGK+VVCERGGGI R+AKGLVVKNGGG
Sbjct: 361 DFSSKFLPLVYAGKSGIEGSEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGG 420

Query: 421 AAMILINQKPEGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRA 480
           AAMIL+NQKP+GFSTLAEAHVLPTTHLSYEDGLKIK YINSSHNPKASISF+GTLLGNRA
Sbjct: 421 AAMILVNQKPDGFSTLAEAHVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRA 480

Query: 481 TTFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGT 540
           TTFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGT
Sbjct: 481 TTFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGT 540

Query: 541 SMSCPHLSGIAALIKSYHPNWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSG 600
           SMSCPHLSGIAALIKS HPNWSPAAIKSAIMTSADVRNPQGKPI+DQDLKPANFFAMGSG
Sbjct: 541 SMSCPHLSGIAALIKSNHPNWSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSG 600

Query: 601 HVNPSKAANPGLVYDIQPDDYIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSF 660
           HVNPSKAANPGLVYDIQPDDY+PYLCHLYTDAQVSIIVR+QVTCSTV RIREGDLNYPSF
Sbjct: 601 HVNPSKAANPGLVYDIQPDDYVPYLCHLYTDAQVSIIVRRQVTCSTVSRIREGDLNYPSF 660

Query: 661 AVSLEAGSQTFNRTVTNVGDANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFS 720
           AVSL A SQ FNRTVTNVGDANSVYY IV+APAGVSV+VTP NLKFSKLNEK+TYSVTFS
Sbjct: 661 AVSLGADSQAFNRTVTNVGDANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFS 720

Query: 721 RIDFVRTTSEFSEGYLIWVSKKLMVRSPISVKLT 755
           RIDFVRT SEFSEGYLIWVS K +VRSPISVKLT
Sbjct: 721 RIDFVRTRSEFSEGYLIWVSNKHIVRSPISVKLT 754

BLAST of Cmc09g0257011 vs. NCBI nr
Match: XP_038906452.1 (subtilisin-like protease 4 [Benincasa hispida])

HSP 1 Score: 1211.8 bits (3134), Expect = 0.0e+00
Identity = 614/734 (83.65%), Postives = 669/734 (91.14%), Query Frame = 0

Query: 21  MKTELSSTSN-TNLQTYIVHVKQPVLEILGDTIDLQNWYTSFLPETIEASSDEQPRLLYS 80
           MK+ELS TSN   LQTYIVHVK+  L ILGD IDL++WYTSFLPETI+ +S+EQ RLLYS
Sbjct: 1   MKSELSGTSNQIKLQTYIVHVKESELRILGDAIDLESWYTSFLPETIK-TSNEQSRLLYS 60

Query: 81  YRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNTHFGLWKNSNF 140
           +R+VMSGFSARLT E+VKAMEEK+GFISA PET LNLHTTHTPEYLGLN  FGLWK+SNF
Sbjct: 61  FRNVMSGFSARLT-EEVKAMEEKEGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNF 120

Query: 141 GKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNFV 200
           GKGVIIGVLDTGI P+HPSFND+GM  PPAKWKGRCEFGASICNNKLIGARTFN AN+ +
Sbjct: 121 GKGVIIGVLDTGISPDHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNRANSAL 180

Query: 201 IGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSS 260
           +G+SP DENGHGTHTASTAAGTFV+GAEALGNARGKAVGMAPLAH+AMYKVCSP GCSSS
Sbjct: 181 MGESPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGMAPLAHLAMYKVCSPKGCSSS 240

Query: 261 DILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAIGAFAAIKKGIFVSCSAGNSGPSKNT 320
           DILAALDAAI DGVDVLSLSLGAPS PF+ D IAIGAFAAIK GIFVSCSAGNSGPSK+T
Sbjct: 241 DILAALDAAIHDGVDVLSLSLGAPSRPFYIDNIAIGAFAAIKMGIFVSCSAGNSGPSKST 300

Query: 321 LANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEG 380
           LANEAPWILTVGASTIDR I+ALA+LG+GEV+ GESLFQPR+FPS FLPLVYAG SG++G
Sbjct: 301 LANEAPWILTVGASTIDRTIMALARLGNGEVHVGESLFQPRDFPSSFLPLVYAGSSGVKG 360

Query: 381 SEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAEA 440
           SE+C+QGSL  LNV GK+VVCERGGGI R+AKG+VVKN GGAAMI+INQK + FSTLAEA
Sbjct: 361 SEFCVQGSLTNLNVKGKMVVCERGGGIGRIAKGMVVKNAGGAAMIIINQKADEFSTLAEA 420

Query: 441 HVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQAS 500
           H+LP TH+SYE GLKIK+YI SS NP ASISF+GTL+G+RATTFSPAMASFSSRGPC  S
Sbjct: 421 HILPATHVSYEAGLKIKSYIRSSQNPMASISFEGTLIGSRATTFSPAMASFSSRGPCLPS 480

Query: 501 PGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYHP 560
           PGILKPDITGPGVNILAAWPF L+N  NTN KSTFNVISGTSMSCPHLSGIAALIKS HP
Sbjct: 481 PGILKPDITGPGVNILAAWPFSLDN--NTNKKSTFNVISGTSMSCPHLSGIAALIKSVHP 540

Query: 561 NWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPD 620
           NWSPAAIKSAIMTSADVRN QG+PI+DQDLKPANFFAMGSGHVNPSKAA+PGLVYDIQPD
Sbjct: 541 NWSPAAIKSAIMTSADVRNRQGRPIVDQDLKPANFFAMGSGHVNPSKAADPGLVYDIQPD 600

Query: 621 DYIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLEAGSQTFNRTVTNVG 680
           DYIPYLC LY+DAQVSIIVR++VTCST+ RIREGDLNYPSFAV+L   SQ FNRTVTNVG
Sbjct: 601 DYIPYLCGLYSDAQVSIIVRRKVTCSTISRIREGDLNYPSFAVTL-GPSQMFNRTVTNVG 660

Query: 681 DANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIWV 740
           DANS+Y  IVEAP GVSVKVTP+NLKFS++ EKVTYSVTFSR D VRTTSEFSEGYLIWV
Sbjct: 661 DANSIYSAIVEAPIGVSVKVTPNNLKFSRVIEKVTYSVTFSRDDLVRTTSEFSEGYLIWV 720

Query: 741 SKKLMVRSPISVKL 754
           S K MVRSPISVKL
Sbjct: 721 SNKHMVRSPISVKL 729

BLAST of Cmc09g0257011 vs. NCBI nr
Match: KAA0042821.1 (subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa])

HSP 1 Score: 1085.1 bits (2805), Expect = 0.0e+00
Identity = 543/757 (71.73%), Postives = 642/757 (84.81%), Query Frame = 0

Query: 1   MVFLPFICIFFFLNFHGYVAMKTELSSTS-NTNLQTYIVHVKQPVLEILGDTID--LQNW 60
           MV L F+ IFF LN   +VA K EL +T   + LQTYIVHV+QP + ++G++ +  +++W
Sbjct: 1   MVVLRFLSIFFLLNIESHVAAKVELPTTPLQSKLQTYIVHVEQPKIRLVGESSNDHIESW 60

Query: 61  YTSFLPETIEASSDEQPRLLYSYRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLH 120
           Y SF+P++ E ++ EQP+LLYSYR+VMSGFSARLT EQVKAME+KDGFISAMPET ++LH
Sbjct: 61  YMSFIPKSTE-TTVEQPQLLYSYRNVMSGFSARLTIEQVKAMEKKDGFISAMPETIMSLH 120

Query: 121 TTHTPEYLGLNTHFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEF 180
           TTHTPEYLGLN  FG+WKNSNFGKGVI+GVLDTGIHPNHPSFNDEGMS PPAKWKG+CEF
Sbjct: 121 TTHTPEYLGLNQQFGIWKNSNFGKGVIVGVLDTGIHPNHPSFNDEGMSPPPAKWKGKCEF 180

Query: 181 GASICNNKLIGARTFNLANNFVIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAV 240
            +S+CNNKLIGARTFNL NNF++ +SPNDE GHGTHTASTAAG FV+ AEALGNA+GKA 
Sbjct: 181 NSSLCNNKLIGARTFNLGNNFLMEESPNDEKGHGTHTASTAAGAFVEDAEALGNAKGKAA 240

Query: 241 GMAPLAHIAMYKVCSPNGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAIGAF 300
           G+APLAH+A+YKVCS   C SSD+ A +DAAIDDGVDVLS+SLG+ S PFF+D IA+  F
Sbjct: 241 GIAPLAHLAIYKVCSGKRCPSSDVFAGIDAAIDDGVDVLSISLGSRSVPFFKDNIAVATF 300

Query: 301 AAIKKGIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLF 360
            AI+KGIFVS SAGNSGP  +TL+N+APWILTVGASTI+R+IVA+AKLG+GE Y GESL+
Sbjct: 301 GAIQKGIFVSSSAGNSGPLNSTLSNDAPWILTVGASTINRRIVAVAKLGNGEQYEGESLY 360

Query: 361 QPRNFPSKFLPLVYAGKSGIEGSEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKN 420
           QP +FPSKFLPLVYAG    +   +C +GSLE ++V GKVVVCE  GG+ RVAKGLVVKN
Sbjct: 361 QPNDFPSKFLPLVYAGNRENKTYAFCGEGSLENMDVKGKVVVCEGKGGVGRVAKGLVVKN 420

Query: 421 GGGAAMILINQKPEGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLG 480
            GGAAMILINQ+ +GFSTL+EAHVLP TH+SY+ G+ IK+YINSS NP ASISFKGT++G
Sbjct: 421 AGGAAMILINQEEDGFSTLSEAHVLPATHVSYKAGVLIKSYINSSQNPTASISFKGTVIG 480

Query: 481 NRATTFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVI 540
           +     +P+MASFSSRGPC  SPGILKPDITGPGVNILAAWPFPL+N  +TNTKSTFNVI
Sbjct: 481 DGDDFSAPSMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFPLDN--DTNTKSTFNVI 540

Query: 541 SGTSMSCPHLSGIAALIKSYHPNWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAM 600
           SGTSMSCPHLSGIAALIKS HPNWSPAAIKSAIMT+A+++ PQG+PI+DQDL+PANFFAM
Sbjct: 541 SGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTTANIKTPQGEPIVDQDLQPANFFAM 600

Query: 601 GSGHVNPSKAANPGLVYDIQPDDYIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNY 660
           G+GHVNPSKAA+PGLVYDIQPDDYIPYLC LY D +VSIIV + V C  VP IREGDLNY
Sbjct: 601 GAGHVNPSKAADPGLVYDIQPDDYIPYLCGLYKDEEVSIIVHRTVICGLVPSIREGDLNY 660

Query: 661 PSFAVSLEAGSQTFNRTVTNVGDANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSV 720
           PSF+V+L  G QTF RTVTNVG+ANSVY  IVEAP GVS+ V P  L FS++N+ +T++V
Sbjct: 661 PSFSVAL-GGLQTFKRTVTNVGEANSVYTAIVEAPLGVSMTVMPRKLFFSRVNQTMTFTV 720

Query: 721 TFSRIDFVRTTSEFSEGYLIWV--SKKLMVRSPISVK 753
           TF+RI +V+   EF EGYL WV  SKK +VRSP+SVK
Sbjct: 721 TFNRIRWVKIVGEFGEGYLKWVSKSKKYVVRSPVSVK 753

BLAST of Cmc09g0257011 vs. ExPASy Swiss-Prot
Match: A9QY38 (Subtilisin-like protease 4 OS=Lotus japonicus OX=34305 GN=SBTM4 PE=2 SV=1)

HSP 1 Score: 888.6 bits (2295), Expect = 4.8e-257
Identity = 463/762 (60.76%), Postives = 576/762 (75.59%), Query Frame = 0

Query: 1   MVFLPFICIFFFLNFHGYVAMKTELSSTSNTNLQT-----YIVHVKQPVLEILGDTIDLQ 60
           M +  FI + F L FH + A  +EL +T+  + +T     YI+HV  P  ++L ++ DL+
Sbjct: 1   MDYFLFIALTFLLTFHVHNAQGSELPTTTTESTETSSSKIYIIHVTGPEGKMLTESEDLE 60

Query: 61  NWYTSFLPETIEASSDEQPRLLYSYRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLN 120
           +WY SFLP T+  SS+EQPR++YSY++V+ GF+A LT+E++ A+E+K+GFISA P+  L+
Sbjct: 61  SWYHSFLPPTL-MSSEEQPRVIYSYKNVLRGFAASLTQEELSAVEKKNGFISAHPQRVLH 120

Query: 121 LHTTHTPEYLGLNTHFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRC 180
             TTHTP++LGL    G+WK SNFGKGVIIGVLD+GI P HPSF+D G+  PP KWKGRC
Sbjct: 121 RQTTHTPKFLGLQQDTGVWKESNFGKGVIIGVLDSGITPGHPSFSDVGIPPPPPKWKGRC 180

Query: 181 EFGASICNNKLIGARTFNLANNFVIGK---SPNDENGHGTHTASTAAGTFVKGAEALGNA 240
           +   + CNNKLIGAR FNLA   + GK   +P DE+GHGTHTASTAAG FV  AE LGNA
Sbjct: 181 DLNVTACNNKLIGARAFNLAAEAMNGKKAEAPIDEDGHGTHTASTAAGAFVNYAEVLGNA 240

Query: 241 RGKAVGMAPLAHIAMYKVCSPNGCSSSDILAALDAAIDDGVDVLSLSLG-APSTPFFRDT 300
           +G A GMAP AH+A+YKVC    C  SDILAALDAA++DGVDV+S+SLG +   PFF D+
Sbjct: 241 KGTAAGMAPHAHLAIYKVCFGEDCPESDILAALDAAVEDGVDVISISLGLSEPPPFFNDS 300

Query: 301 IAIGAFAAIKKGIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLGSGEVY 360
            AIGAFAA++KGIFVSC+AGNSGP  +++ N APWILTVGASTIDR+IVA AKLG+G+ +
Sbjct: 301 TAIGAFAAMQKGIFVSCAAGNSGPFNSSIVNAAPWILTVGASTIDRRIVATAKLGNGQEF 360

Query: 361 TGESLFQPRNFPSKFLPLVYAGKSGIEGSEYCIQGSLEKLNVTGKVVVCERGGGISRVAK 420
            GES+FQP +F    LPL YAGK+G E S +C  GSL+     GKVV+CERGGGI+R+AK
Sbjct: 361 DGESVFQPSSFTPTLLPLAYAGKNGKEESAFCANGSLDDSAFRGKVVLCERGGGIARIAK 420

Query: 421 GLVVKNGGGAAMILINQKPEGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISF 480
           G  VK  GGAAMIL+N +   FS  A+ H LP TH+SY  G++IKAYINS+  P A+I F
Sbjct: 421 GEEVKRAGGAAMILMNDETNAFSLSADVHALPATHVSYAAGIEIKAYINSTATPTATILF 480

Query: 481 KGTLLGNRATTFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTK 540
           KGT++GN   + +PA+ASFSSRGP   SPGILKPDI GPGVNILAAWPFPL+N  +T++K
Sbjct: 481 KGTVIGN---SLAPAVASFSSRGPNLPSPGILKPDIIGPGVNILAAWPFPLSN--STDSK 540

Query: 541 STFNVISGTSMSCPHLSGIAALIKSYHPNWSPAAIKSAIMTSADVRNPQGKPIMDQDLKP 600
            TFN+ SGTSMSCPHLSGIAAL+KS HP+WSPAAIKSAIMTSAD  N   K I+D+ L+P
Sbjct: 541 LTFNIESGTSMSCPHLSGIAALLKSSHPHWSPAAIKSAIMTSADTINLGNKLIVDETLQP 600

Query: 601 ANFFAMGSGHVNPSKAANPGLVYDIQPDDYIPYLCHL-YTDAQVSIIVRKQVTCSTVPRI 660
            + FA GSGHVNPS+A +PGLVYDIQPDDYIPYLC L Y++ +V II  +++ CS    I
Sbjct: 601 TDLFATGSGHVNPSRANDPGLVYDIQPDDYIPYLCGLGYSETEVGIIAHRKIKCSA--SI 660

Query: 661 REGDLNYPSFAVSLEAGSQTFNRTVTNVGDANSVYYPIVEAPAGVSVKVTPSNLKFSKLN 720
            EG+LNYPSF+V L   S+TF RTVTNVG+A+S Y  IV AP GV VKV P  L FS++N
Sbjct: 661 PEGELNYPSFSVEL-GSSKTFTRTVTNVGEAHSSYDLIVAAPQGVDVKVQPYKLNFSEVN 720

Query: 721 EKVTYSVTFSRIDFVRTTSEFSEGYLIWVSKKLMVRSPISVK 753
           +K TYSVTFSR      T E+++G+L WVS K  VRSPISVK
Sbjct: 721 QKETYSVTFSRTGLGNKTQEYAQGFLKWVSTKHTVRSPISVK 753

BLAST of Cmc09g0257011 vs. ExPASy Swiss-Prot
Match: A0A0M3R8G2 (Subtilisin-like protease OS=Petunia hybrida OX=4102 GN=SBT PE=2 SV=1)

HSP 1 Score: 835.9 bits (2158), Expect = 3.7e-241
Identity = 443/753 (58.83%), Postives = 551/753 (73.17%), Query Frame = 0

Query: 7   ICIF---FFLNFHGYVAMKTELSSTSNTNLQTYIVHVKQPVLEILGDTIDLQNWYTSFLP 66
           ICIF   F L+FH   A +       +T LQTYIVHV +P  ++L ++ DL+++Y SFLP
Sbjct: 4   ICIFSIAFLLSFHLTTAQR-------STGLQTYIVHVDKPDAQVLANSADLESYYNSFLP 63

Query: 67  ETIEASSDEQPRLLYSYRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTPE 126
            T+ + S+   R+++SY HV +GF+A+L+ E+VK ME+K GF+SA  E  L LHTTHTP 
Sbjct: 64  ATV-SGSEVPSRIIHSYHHVATGFAAKLSGEEVKEMEKKAGFVSAKLEKVLTLHTTHTPN 123

Query: 127 YLGLNTHFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEF-GASIC 186
           +LGL  + G W+ SN+GKGVIIG+LDTGI P HPSF+D  M SPPAKWKG+CEF G + C
Sbjct: 124 FLGLYQNMGFWQESNYGKGVIIGLLDTGITPGHPSFSDVNMPSPPAKWKGKCEFTGNATC 183

Query: 187 NNKLIGARTFNLANNFVIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPL 246
           N K+IGAR F   +    G  P DE GHGTHTASTAAG FV  A   GNA G AVGMAPL
Sbjct: 184 NKKIIGARNFISGS----GVPPTDEEGHGTHTASTAAGNFVNDANVFGNANGTAVGMAPL 243

Query: 247 AHIAMYKVCSPNGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAIGAFAAIKK 306
           AHIAMYKVCS +GCS +DILAALDAAIDDGVDVLSLSLG  S PF+ D IAIGAFAAI+K
Sbjct: 244 AHIAMYKVCSEDGCSDADILAALDAAIDDGVDVLSLSLGGYSDPFYYDNIAIGAFAAIRK 303

Query: 307 GIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNF 366
           GIFVS SAGN GP  +TL+NEAPWILTVGAST DRKIVA A LG+G+ Y GES FQP +F
Sbjct: 304 GIFVSASAGNDGPLNSTLSNEAPWILTVGASTHDRKIVATAVLGNGQQYDGESAFQPADF 363

Query: 367 PSKFLPLVYAGKSGIEGSEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAA 426
           P   LPLVY G S  E + +C  GSL+K +V GKVVVC+RGG ++R+ K   VK+ GGAA
Sbjct: 364 PHTLLPLVYPGTSD-EEAAFCSSGSLDKFDVKGKVVVCDRGGDVARLEKSQTVKDAGGAA 423

Query: 427 MILINQKPEGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRATT 486
           MIL N + +G  T A+AHVLP TH+ Y  G  IK+YINS+  P A I FKGT++G ++  
Sbjct: 424 MILANLEIDGEGTFADAHVLPATHVGYAAGEMIKSYINSTSTPTAGILFKGTIIGFKS-- 483

Query: 487 FSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSM 546
            SP+++SFSSRGP  ASPGI+KPDI GPGVNILAAWP  + N T T+   TFN+ISGTSM
Sbjct: 484 -SPSVSSFSSRGPNLASPGIVKPDIIGPGVNILAAWPVSVENKTGTDL--TFNIISGTSM 543

Query: 547 SCPHLSGIAALIKSYHPNWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSGHV 606
           SCPHLSGI AL+KS HP+WSPAAIKSAIMTSAD  N +G+PI+D+   PA+ FA G+GHV
Sbjct: 544 SCPHLSGIVALLKSAHPDWSPAAIKSAIMTSADQSNLEGQPILDERNLPADIFATGAGHV 603

Query: 607 NPSKAANPGLVYDIQPDDYIPYLCHL-YTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFA 666
           NPSKA++PGL+YDIQ +DYI YLC L Y +  + +IV++ V C +   I E +LNYPSF+
Sbjct: 604 NPSKASDPGLIYDIQLEDYIQYLCGLGYREKDIGLIVQETVKCES--SISEAELNYPSFS 663

Query: 667 VSLEAGSQTFNRTVTNVGDANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFSR 726
           + L   +Q + RTVTNVGDA+S Y   +    GV + V P+ L F+K+ ++ TY+V+F++
Sbjct: 664 IILGPKTQNYTRTVTNVGDASSTYTVNIAKTQGVDIVVEPATLVFTKMYQQATYTVSFTQ 723

Query: 727 I-DFVRTTSEFSEGYLIWVSKKLMVRSPISVKL 754
             DF   T  F +G + W S + +VRSPISVKL
Sbjct: 724 SGDF---TDRFVQGAISWSSNEYVVRSPISVKL 733

BLAST of Cmc09g0257011 vs. ExPASy Swiss-Prot
Match: A9QY39 (Subtilisin-like protease 3 OS=Lotus japonicus OX=34305 GN=SBTM3 PE=2 SV=1)

HSP 1 Score: 816.6 bits (2108), Expect = 2.3e-235
Identity = 425/731 (58.14%), Postives = 532/731 (72.78%), Query Frame = 0

Query: 23  TELSSTSNTNLQTYIVHVKQPVLEILGDTIDLQNWYTSFLPETIEASSDEQPRLLYSYRH 82
           TEL     +NL TYIVHV++P + I  D  DL  +Y S LPE+ + ++    R++++YR+
Sbjct: 32  TELEDEDQSNLSTYIVHVRKPQV-IQSD--DLHTFYYSLLPESTKTTNQ---RIVFTYRN 91

Query: 83  VMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNTHFGLWKNSNFGKG 142
           V++GF+ +LT E+ KA+++ +  +SA PE  L+LHTTHTP +LGL    GLWK SN GKG
Sbjct: 92  VVNGFAVKLTPEEAKALQQNEEVVSARPEKILSLHTTHTPSFLGLQQGLGLWKGSNSGKG 151

Query: 143 VIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEF-GASICNNKLIGARTFNLANNFVIG 202
           VIIG+LDTGI P HPSF+DEGM SPPAKW G CEF G   CNNK+IGAR F    N  + 
Sbjct: 152 VIIGILDTGISPFHPSFSDEGMPSPPAKWNGICEFTGKRTCNNKIIGARNFVKTKNLTL- 211

Query: 203 KSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSSDI 262
             P D+ GHGTHTASTAAG  V+GA   GNA G AVGMAP AHIAMYKVC   GCS S I
Sbjct: 212 --PFDDVGHGTHTASTAAGRPVQGANVYGNANGTAVGMAPDAHIAMYKVCGLVGCSESAI 271

Query: 263 LAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAIGAFAAIKKGIFVSCSAGNSGPSKNTLA 322
           LA +D A+DDGVDVLSLSLG PS PFF D IA+GAF AI+KGIFVSCSA NSGP+ ++L+
Sbjct: 272 LAGMDTAVDDGVDVLSLSLGGPSGPFFEDPIALGAFGAIQKGIFVSCSAANSGPAYSSLS 331

Query: 323 NEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEG-S 382
           NEAPWILTVGAS+IDR I+A AKLG+G+ Y G+S+FQP++F    LPLVYAG +G    S
Sbjct: 332 NEAPWILTVGASSIDRTIMATAKLGNGKEYVGQSVFQPKDFAPSLLPLVYAGANGNNNFS 391

Query: 383 EYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAEAH 442
            +C   SL + +V GKVV+CE GG + RV KG  VK+ GGAAMIL+N   E F+ +A+ H
Sbjct: 392 VFCAPESLNRSDVEGKVVLCEDGGFVPRVFKGKAVKDAGGAAMILMNSVLEDFNPIADVH 451

Query: 443 VLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQASP 502
           VLP  H+SYE GL +K YINS+  P A+I F+GT++GN     +P + SFSSRGP +ASP
Sbjct: 452 VLPAVHISYEAGLALKEYINSTSTPTATILFEGTVIGN---LLAPQVTSFSSRGPSKASP 511

Query: 503 GILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYHPN 562
           GILKPDI GPG+NILAAWP  L+N+T       FN+ISGTSMSCPHLSGIAAL+K+ HP+
Sbjct: 512 GILKPDIIGPGLNILAAWPVSLDNSTT----PPFNIISGTSMSCPHLSGIAALLKNSHPD 571

Query: 563 WSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD 622
           WSPAAIKSAIMT+A   N  G PI+DQ L PA+ FA G+GHVNP KA +PGLVYDI+P+D
Sbjct: 572 WSPAAIKSAIMTTASQVNLGGTPILDQRLVPADVFATGAGHVNPVKANDPGLVYDIEPND 631

Query: 623 YIPYLCHL-YTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLEAGSQTFNRTVTNVG 682
           YIPYLC L YTD +V +I++++V CS V  I E +LNYPSF++ L   +Q + RTV NVG
Sbjct: 632 YIPYLCGLNYTDREVGVILQQRVRCSEVNHIAEAELNYPSFSILLGNTTQLYTRTVANVG 691

Query: 683 DANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIWV 742
            ANS Y   +  P GV + ++P+ L F+++ +K+TYSV+F      R    F++G L WV
Sbjct: 692 PANSTYTAEIGVPVGVGMSLSPAQLTFTEVGQKLTYSVSFIPFSEDRDNHTFAQGSLKWV 746

Query: 743 SKKLMVRSPIS 751
           S K  VRSPIS
Sbjct: 752 SGKYSVRSPIS 746

BLAST of Cmc09g0257011 vs. ExPASy Swiss-Prot
Match: A9QY40 (Subtilisin-like protease 1 OS=Lotus japonicus OX=34305 GN=SBTM1 PE=2 SV=1)

HSP 1 Score: 803.5 bits (2074), Expect = 2.0e-231
Identity = 425/727 (58.46%), Postives = 532/727 (73.18%), Query Frame = 0

Query: 32  NLQTYIVHVKQPVLE-ILGDTIDLQNWYTSFLPETIEASSDEQPRLLYSYRHVMSGFSAR 91
           NL TYIVHVK+  +E  L  T +L  W+ SFLPET       + R+++SYR+V SGF+ R
Sbjct: 37  NLTTYIVHVKKLEIEGPLQSTEELHTWHHSFLPET-----SNKDRMVFSYRNVASGFAVR 96

Query: 92  LTKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNTHFGLWKNSNFGKGVIIGVLDT 151
           LT E+  A++EK+  +S  PE TL+LHTTHTP +LGL    GLW +SN GKGVIIGV+DT
Sbjct: 97  LTPEEANALQEKEEVMSIRPERTLSLHTTHTPSFLGLRQGQGLWNDSNLGKGVIIGVIDT 156

Query: 152 GIHPNHPSFNDEGMSSPPAKWKGRCEF-GASICNNKLIGARTFNLANNFVIGKSPNDENG 211
           GI+P H SFNDEGM  PPAKWKG CEF G S+CNNKLIGAR  NL  +  I + P ++  
Sbjct: 157 GIYPFHLSFNDEGMPPPPAKWKGHCEFTGGSVCNNKLIGAR--NLVKS-AIQEPPYEDFF 216

Query: 212 HGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCS---PNGCSSSDILAALD 271
           HGTHTA+ AAG FV+GA   GNARG A GMAP AH+A+YKVCS    + C  S ILAA+D
Sbjct: 217 HGTHTAAEAAGRFVEGASVFGNARGTAAGMAPDAHLAIYKVCSSKVKDECPESAILAAMD 276

Query: 272 AAIDDGVDVLSLSLGAPSTPFFRDTIAIGAFAAIKKGIFVSCSAGNSGPSKNTLANEAPW 331
            AI+DGVDVLSLSLG  S PFF D IAIGAFAA +KGIFVSCSA NSGP  ++L+NEAPW
Sbjct: 277 IAIEDGVDVLSLSLGLGSLPFFEDPIAIGAFAATQKGIFVSCSAANSGPHYSSLSNEAPW 336

Query: 332 ILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEGSEYCIQG 391
           ILTVGASTIDRKI A AKLG+G  Y GE+LFQP++F S+ LPLVYA       S  C  G
Sbjct: 337 ILTVGASTIDRKISASAKLGNGAEYEGETLFQPKDFSSQLLPLVYAAAEKNNSSALCAPG 396

Query: 392 SLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAEAHVLPTTH 451
           SL  +NV GKVVVC+ GGGI  +AKG  V + GG+AMIL N +  GF+TLA AHVLP  H
Sbjct: 397 SLRNINVKGKVVVCDLGGGIPFIAKGQEVLDAGGSAMILANIENFGFTTLANAHVLPAVH 456

Query: 452 LSYEDGLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQASPGILKPD 511
           +SY   L IKAYINS++ P A++ F+GT++G+   + +P++A+FSSRGP Q SPGILKPD
Sbjct: 457 VSYAASLAIKAYINSTYTPTATVLFQGTIIGD---SLAPSVAAFSSRGPSQQSPGILKPD 516

Query: 512 ITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYHPNWSPAAI 571
           I GPGVNILAAW   ++N         F++ISGTSMSCPHLSGIAAL+KS HP+WSPAAI
Sbjct: 517 IIGPGVNILAAWAVSVDNKI-----PAFDIISGTSMSCPHLSGIAALLKSAHPDWSPAAI 576

Query: 572 KSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYIPYLC 631
           KSAIMT+A+  N +G PI+DQ L+PA+ FA G+GHVNP +A +PGLVYDIQP+DY+PYLC
Sbjct: 577 KSAIMTTANTLNLRGLPILDQRLQPADIFATGAGHVNPVRANDPGLVYDIQPEDYVPYLC 636

Query: 632 HL-YTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLEAGSQTFNRTVTNVGDANSVY 691
            L Y+D +V+IIV++ V C  V  I + +LNYPSF++ L + SQ + RT+TNVG ANS Y
Sbjct: 637 GLGYSDREVTIIVQRSVRCFNVKSIAQAELNYPSFSILLGSDSQFYTRTLTNVGPANSTY 696

Query: 692 YPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTF-SRIDFVRTTSEFSEGYLIWVSKKLM 751
              ++ P  + + V+PS + F+++N+KV Y V F  +I   R    F++G + WVS K +
Sbjct: 697 TVKIDVPLAMGISVSPSQITFTQVNQKVAYFVDFIPQIKENRGNHTFAQGAITWVSDKHV 747

BLAST of Cmc09g0257011 vs. ExPASy Swiss-Prot
Match: G7KEU7 (Subtilisin-like protease OS=Medicago truncatula OX=3880 GN=SBT PE=2 SV=1)

HSP 1 Score: 783.5 bits (2022), Expect = 2.2e-225
Identity = 414/728 (56.87%), Postives = 519/728 (71.29%), Query Frame = 0

Query: 32  NLQTYIVHVKQPVLEILGDTIDLQNWYTSFLPETIEASSDEQPRLLYSYRHVMSGFSARL 91
           NL TYIVHVK+        + DL +WY SFLP+T       + R+++SYR V SGF+ +L
Sbjct: 34  NLMTYIVHVKKSENVASHQSEDLHSWYHSFLPQTF----PHKERMVFSYRKVASGFAVKL 93

Query: 92  TKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNTHFGLWKNSNFGKGVIIGVLDTG 151
           T E+ K+++EK   +SA PE TL LHTTHTP +LGL    GLW + N GKGVIIG++DTG
Sbjct: 94  TPEEAKSLQEKGEIVSARPERTLELHTTHTPTFLGLKQGQGLWSDDNLGKGVIIGIIDTG 153

Query: 152 IHPNHPSFNDEGMSSPPAKWKGRCEF-GASICNNKLIGARTFNLANNFVIGKSPNDENGH 211
           I P HPSFNDEGM  PPAKWKG CEF G  +CNNKLIGAR  NL  +  I + P +   H
Sbjct: 154 IFPLHPSFNDEGMPPPPAKWKGHCEFTGGQVCNNKLIGAR--NLVKS-AIQEPPFENFFH 213

Query: 212 GTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPN-GCSSSDILAALDAAI 271
           GTHTA+ AAG F++ A   GNA+G A GMAP AH+A+YKVC+   GC+ S ILAA+D AI
Sbjct: 214 GTHTAAEAAGRFIEDASVFGNAKGVAAGMAPNAHLAIYKVCNDKIGCTESAILAAMDIAI 273

Query: 272 DDGVDVLSLSLGAPSTPFFRDTIAIGAFAAIKKGIFVSCSAGNSGPSKNTLANEAPWILT 331
           +DGVDVLSLSLG  S PFF D IAIGAFAA + G+FVSCSA NSGP  +TL+NEAPWILT
Sbjct: 274 EDGVDVLSLSLGLGSLPFFEDPIAIGAFAATQNGVFVSCSAANSGPGYSTLSNEAPWILT 333

Query: 332 VGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSG----IEGSEYCIQ 391
           VGASTIDRKIVA AKLG+GE Y GE+LFQP++F  + LPLVY G  G     +    C+ 
Sbjct: 334 VGASTIDRKIVASAKLGNGEEYEGETLFQPKDFSQQLLPLVYPGSFGYGNQTQNQSLCLP 393

Query: 392 GSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAEAHVLPTT 451
           GSL+ ++++GKVV+C+  G +S + KG  V N GG AMIL N +  GFST A AHVLP  
Sbjct: 394 GSLKNIDLSGKVVLCD-VGNVSSIVKGQEVLNSGGIAMILANSEALGFSTFAIAHVLPAV 453

Query: 452 HLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQASPGILKP 511
            +SY  GL IK+YI S++NP A++ FKGT++G+   + +P++  FSSRGP Q SPGILKP
Sbjct: 454 EVSYAAGLTIKSYIKSTYNPTATLIFKGTIIGD---SLAPSVVYFSSRGPSQESPGILKP 513

Query: 512 DITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYHPNWSPAA 571
           DI GPGVNILAAW   ++N         F+++SGTSMSCPHLSGIAALIKS HP+WSPAA
Sbjct: 514 DIIGPGVNILAAWAVSVDNKI-----PAFDIVSGTSMSCPHLSGIAALIKSSHPDWSPAA 573

Query: 572 IKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYIPYL 631
           IKSAIMT+A+  N  G PI+DQ L PA+ FA G+GHVNP KA +PGLVYDI+P+DY+PYL
Sbjct: 574 IKSAIMTTANTLNLGGIPILDQRLFPADIFATGAGHVNPVKANDPGLVYDIEPEDYVPYL 633

Query: 632 CHL-YTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLEAGSQTFNRTVTNVGDANSV 691
           C L Y+D ++ +IV+ +V CS V  I E  LNYPSF++ L + SQ + RT+TNVG ANS 
Sbjct: 634 CGLGYSDKEIEVIVQWKVKCSNVKSIPEAQLNYPSFSILLGSDSQYYTRTLTNVGFANST 693

Query: 692 YYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTF-SRIDFVRTTSEFSEGYLIWVSKKL 751
           Y   +E P  + + V PS + F+++NEKV++SV F  +I   R    F +G L WVS + 
Sbjct: 694 YKVELEVPLALGMSVNPSEITFTEVNEKVSFSVEFIPQIKENRRNHTFGQGSLTWVSDRH 745

BLAST of Cmc09g0257011 vs. ExPASy TrEMBL
Match: A0A1S3AT16 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103482683 PE=3 SV=1)

HSP 1 Score: 1499.2 bits (3880), Expect = 0.0e+00
Identity = 751/754 (99.60%), Postives = 752/754 (99.73%), Query Frame = 0

Query: 1   MVFLPFICIFFFLNFHGYVAMKTELSSTSNTNLQTYIVHVKQPVLEILGDTIDLQNWYTS 60
           MVFLPFICIFFFLNFHGYVAMKTELSSTSNTNLQTYIVHVKQPVLEILGDTIDLQNWYTS
Sbjct: 1   MVFLPFICIFFFLNFHGYVAMKTELSSTSNTNLQTYIVHVKQPVLEILGDTIDLQNWYTS 60

Query: 61  FLPETIEASSDEQPRLLYSYRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTH 120
           FLPETIEASSDEQPRLLYSYRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTH
Sbjct: 61  FLPETIEASSDEQPRLLYSYRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTH 120

Query: 121 TPEYLGLNTHFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGAS 180
           TPEYLGLN HFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGAS
Sbjct: 121 TPEYLGLNKHFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGAS 180

Query: 181 ICNNKLIGARTFNLANNFVIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMA 240
           ICNNKLIGARTFNLANNFVIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMA
Sbjct: 181 ICNNKLIGARTFNLANNFVIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMA 240

Query: 241 PLAHIAMYKVCSPNGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAIGAFAAI 300
           PLAHIAMYKVCSPNGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIA+GAFAAI
Sbjct: 241 PLAHIAMYKVCSPNGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAMGAFAAI 300

Query: 301 KKGIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPR 360
           KKGIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPR
Sbjct: 301 KKGIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPR 360

Query: 361 NFPSKFLPLVYAGKSGIEGSEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGG 420
           NFPSKFLPLVYAGKSGIEGSEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGG
Sbjct: 361 NFPSKFLPLVYAGKSGIEGSEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGG 420

Query: 421 AAMILINQKPEGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRA 480
           AAMILINQKPEGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRA
Sbjct: 421 AAMILINQKPEGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRA 480

Query: 481 TTFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGT 540
           TTFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGT
Sbjct: 481 TTFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGT 540

Query: 541 SMSCPHLSGIAALIKSYHPNWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSG 600
           SMSCPHLSGIAALIKSYHPNWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSG
Sbjct: 541 SMSCPHLSGIAALIKSYHPNWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSG 600

Query: 601 HVNPSKAANPGLVYDIQPDDYIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSF 660
           HVNPSKAANPGLVYDIQPDDYIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSF
Sbjct: 601 HVNPSKAANPGLVYDIQPDDYIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSF 660

Query: 661 AVSLEAGSQTFNRTVTNVGDANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFS 720
           AVSL AGSQTFNRTVTNVGDANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFS
Sbjct: 661 AVSLGAGSQTFNRTVTNVGDANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFS 720

Query: 721 RIDFVRTTSEFSEGYLIWVSKKLMVRSPISVKLT 755
           RIDFVRTTSEFSEGYLIWVSKKLMVRSPISVKLT
Sbjct: 721 RIDFVRTTSEFSEGYLIWVSKKLMVRSPISVKLT 754

BLAST of Cmc09g0257011 vs. ExPASy TrEMBL
Match: A0A5A7THE4 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003290 PE=3 SV=1)

HSP 1 Score: 1466.8 bits (3796), Expect = 0.0e+00
Identity = 734/734 (100.00%), Postives = 734/734 (100.00%), Query Frame = 0

Query: 21  MKTELSSTSNTNLQTYIVHVKQPVLEILGDTIDLQNWYTSFLPETIEASSDEQPRLLYSY 80
           MKTELSSTSNTNLQTYIVHVKQPVLEILGDTIDLQNWYTSFLPETIEASSDEQPRLLYSY
Sbjct: 1   MKTELSSTSNTNLQTYIVHVKQPVLEILGDTIDLQNWYTSFLPETIEASSDEQPRLLYSY 60

Query: 81  RHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNTHFGLWKNSNFG 140
           RHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNTHFGLWKNSNFG
Sbjct: 61  RHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNTHFGLWKNSNFG 120

Query: 141 KGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNFVI 200
           KGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNFVI
Sbjct: 121 KGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNFVI 180

Query: 201 GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSSD 260
           GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSSD
Sbjct: 181 GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSSD 240

Query: 261 ILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAIGAFAAIKKGIFVSCSAGNSGPSKNTL 320
           ILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAIGAFAAIKKGIFVSCSAGNSGPSKNTL
Sbjct: 241 ILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAIGAFAAIKKGIFVSCSAGNSGPSKNTL 300

Query: 321 ANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEGS 380
           ANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEGS
Sbjct: 301 ANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEGS 360

Query: 381 EYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAEAH 440
           EYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAEAH
Sbjct: 361 EYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAEAH 420

Query: 441 VLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQASP 500
           VLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQASP
Sbjct: 421 VLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQASP 480

Query: 501 GILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYHPN 560
           GILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYHPN
Sbjct: 481 GILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYHPN 540

Query: 561 WSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD 620
           WSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD
Sbjct: 541 WSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD 600

Query: 621 YIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLEAGSQTFNRTVTNVGD 680
           YIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLEAGSQTFNRTVTNVGD
Sbjct: 601 YIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLEAGSQTFNRTVTNVGD 660

Query: 681 ANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIWVS 740
           ANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIWVS
Sbjct: 661 ANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIWVS 720

Query: 741 KKLMVRSPISVKLT 755
           KKLMVRSPISVKLT
Sbjct: 721 KKLMVRSPISVKLT 734

BLAST of Cmc09g0257011 vs. ExPASy TrEMBL
Match: A0A0A0KMQ7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157280 PE=3 SV=1)

HSP 1 Score: 1381.7 bits (3575), Expect = 0.0e+00
Identity = 687/734 (93.60%), Postives = 710/734 (96.73%), Query Frame = 0

Query: 21  MKTELSSTSNTNLQTYIVHVKQPVLEILGDTIDLQNWYTSFLPETIEASSDEQPRLLYSY 80
           MKTEL S SNTNLQTYIVHVKQP +EILGDTIDLQNWYTSFLPETIEASS+EQ RLLYSY
Sbjct: 1   MKTELWSASNTNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSY 60

Query: 81  RHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNTHFGLWKNSNFG 140
           RHV+SGFSARLTKEQVK MEEKDGFISAMPETTLNLHTTHTPEYLGLN HFGLWKNSNFG
Sbjct: 61  RHVISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFG 120

Query: 141 KGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNFVI 200
           KGVIIGVLDTGIHPNHPSFNDEGM SPPAKWKGRCEFGASICNNKLIGARTFNLANN  I
Sbjct: 121 KGVIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSI 180

Query: 201 GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSSD 260
           GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIA+YKVCSP GCSSSD
Sbjct: 181 GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSSD 240

Query: 261 ILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAIGAFAAIKKGIFVSCSAGNSGPSKNTL 320
           ILAALDAAIDDGVDVLSLSLGAPSTPFF+DTIA+GAFAAIKKGIFVSCSAGNSGPSKNTL
Sbjct: 241 ILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTL 300

Query: 321 ANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEGS 380
           ANEAPWILTVGASTIDRKIVALAKL SG+V+TGESLFQPR+F SKFLPLVYAGKSGIEGS
Sbjct: 301 ANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGS 360

Query: 381 EYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAEAH 440
           EYC++GSLEKLNVTGK+VVCERGGGI R+AKGLVVKNGGGAAMIL+NQKP+GFSTLAEAH
Sbjct: 361 EYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAH 420

Query: 441 VLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQASP 500
           VLPTTHLSYEDGLKIK YINSSHNPKASISF+GTLLGNRATTFSPAMASFSSRGPCQASP
Sbjct: 421 VLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASP 480

Query: 501 GILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYHPN 560
           GILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKS HPN
Sbjct: 481 GILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPN 540

Query: 561 WSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD 620
           WSPAAIKSAIMTSADVRNPQGKPI+DQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD
Sbjct: 541 WSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD 600

Query: 621 YIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLEAGSQTFNRTVTNVGD 680
           Y+PYLCHLYTDAQVSIIVR+QVTCSTV RIREGDLNYPSFAVSL A SQ FNRTVTNVGD
Sbjct: 601 YVPYLCHLYTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGD 660

Query: 681 ANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIWVS 740
           ANSVYY IV+APAGVSV+VTP NLKFSKLNEK+TYSVTFSRIDFVRT SEFSEGYLIWVS
Sbjct: 661 ANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVS 720

Query: 741 KKLMVRSPISVKLT 755
            K +VRSPISVKLT
Sbjct: 721 NKHIVRSPISVKLT 734

BLAST of Cmc09g0257011 vs. ExPASy TrEMBL
Match: A0A5A7TMM4 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003280 PE=3 SV=1)

HSP 1 Score: 1085.1 bits (2805), Expect = 0.0e+00
Identity = 543/757 (71.73%), Postives = 642/757 (84.81%), Query Frame = 0

Query: 1   MVFLPFICIFFFLNFHGYVAMKTELSSTS-NTNLQTYIVHVKQPVLEILGDTID--LQNW 60
           MV L F+ IFF LN   +VA K EL +T   + LQTYIVHV+QP + ++G++ +  +++W
Sbjct: 1   MVVLRFLSIFFLLNIESHVAAKVELPTTPLQSKLQTYIVHVEQPKIRLVGESSNDHIESW 60

Query: 61  YTSFLPETIEASSDEQPRLLYSYRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLH 120
           Y SF+P++ E ++ EQP+LLYSYR+VMSGFSARLT EQVKAME+KDGFISAMPET ++LH
Sbjct: 61  YMSFIPKSTE-TTVEQPQLLYSYRNVMSGFSARLTIEQVKAMEKKDGFISAMPETIMSLH 120

Query: 121 TTHTPEYLGLNTHFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEF 180
           TTHTPEYLGLN  FG+WKNSNFGKGVI+GVLDTGIHPNHPSFNDEGMS PPAKWKG+CEF
Sbjct: 121 TTHTPEYLGLNQQFGIWKNSNFGKGVIVGVLDTGIHPNHPSFNDEGMSPPPAKWKGKCEF 180

Query: 181 GASICNNKLIGARTFNLANNFVIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAV 240
            +S+CNNKLIGARTFNL NNF++ +SPNDE GHGTHTASTAAG FV+ AEALGNA+GKA 
Sbjct: 181 NSSLCNNKLIGARTFNLGNNFLMEESPNDEKGHGTHTASTAAGAFVEDAEALGNAKGKAA 240

Query: 241 GMAPLAHIAMYKVCSPNGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAIGAF 300
           G+APLAH+A+YKVCS   C SSD+ A +DAAIDDGVDVLS+SLG+ S PFF+D IA+  F
Sbjct: 241 GIAPLAHLAIYKVCSGKRCPSSDVFAGIDAAIDDGVDVLSISLGSRSVPFFKDNIAVATF 300

Query: 301 AAIKKGIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLF 360
            AI+KGIFVS SAGNSGP  +TL+N+APWILTVGASTI+R+IVA+AKLG+GE Y GESL+
Sbjct: 301 GAIQKGIFVSSSAGNSGPLNSTLSNDAPWILTVGASTINRRIVAVAKLGNGEQYEGESLY 360

Query: 361 QPRNFPSKFLPLVYAGKSGIEGSEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKN 420
           QP +FPSKFLPLVYAG    +   +C +GSLE ++V GKVVVCE  GG+ RVAKGLVVKN
Sbjct: 361 QPNDFPSKFLPLVYAGNRENKTYAFCGEGSLENMDVKGKVVVCEGKGGVGRVAKGLVVKN 420

Query: 421 GGGAAMILINQKPEGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLG 480
            GGAAMILINQ+ +GFSTL+EAHVLP TH+SY+ G+ IK+YINSS NP ASISFKGT++G
Sbjct: 421 AGGAAMILINQEEDGFSTLSEAHVLPATHVSYKAGVLIKSYINSSQNPTASISFKGTVIG 480

Query: 481 NRATTFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVI 540
           +     +P+MASFSSRGPC  SPGILKPDITGPGVNILAAWPFPL+N  +TNTKSTFNVI
Sbjct: 481 DGDDFSAPSMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFPLDN--DTNTKSTFNVI 540

Query: 541 SGTSMSCPHLSGIAALIKSYHPNWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAM 600
           SGTSMSCPHLSGIAALIKS HPNWSPAAIKSAIMT+A+++ PQG+PI+DQDL+PANFFAM
Sbjct: 541 SGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTTANIKTPQGEPIVDQDLQPANFFAM 600

Query: 601 GSGHVNPSKAANPGLVYDIQPDDYIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNY 660
           G+GHVNPSKAA+PGLVYDIQPDDYIPYLC LY D +VSIIV + V C  VP IREGDLNY
Sbjct: 601 GAGHVNPSKAADPGLVYDIQPDDYIPYLCGLYKDEEVSIIVHRTVICGLVPSIREGDLNY 660

Query: 661 PSFAVSLEAGSQTFNRTVTNVGDANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSV 720
           PSF+V+L  G QTF RTVTNVG+ANSVY  IVEAP GVS+ V P  L FS++N+ +T++V
Sbjct: 661 PSFSVAL-GGLQTFKRTVTNVGEANSVYTAIVEAPLGVSMTVMPRKLFFSRVNQTMTFTV 720

Query: 721 TFSRIDFVRTTSEFSEGYLIWV--SKKLMVRSPISVK 753
           TF+RI +V+   EF EGYL WV  SKK +VRSP+SVK
Sbjct: 721 TFNRIRWVKIVGEFGEGYLKWVSKSKKYVVRSPVSVK 753

BLAST of Cmc09g0257011 vs. ExPASy TrEMBL
Match: A0A1S3AUP1 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103483066 PE=3 SV=1)

HSP 1 Score: 1073.9 bits (2776), Expect = 2.8e-310
Identity = 538/752 (71.54%), Postives = 635/752 (84.44%), Query Frame = 0

Query: 6   FICIFFFLNFHGYVAMKTELSSTS-NTNLQTYIVHVKQPVLEILGDTID--LQNWYTSFL 65
           F   FF LN   +VA K EL +T   + LQTYIVHV+QP + ++G++ +  +++WY SF+
Sbjct: 15  FPLYFFLLNIESHVAAKVELPTTPLQSKLQTYIVHVEQPKIRLVGESSNDHIESWYMSFI 74

Query: 66  PETIEASSDEQPRLLYSYRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTP 125
           P++ E ++ EQP+LLYSYR+VMSGFSARLT EQVKAME+KDGFISAMPET ++LHTTHTP
Sbjct: 75  PKSTE-TTVEQPQLLYSYRNVMSGFSARLTIEQVKAMEKKDGFISAMPETIMSLHTTHTP 134

Query: 126 EYLGLNTHFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASIC 185
           EYLGLN  FG+WKNSNFGKGVI+GVLDTGIHPNHPSFNDEGMS PPAKWKG+CEF +S+C
Sbjct: 135 EYLGLNQQFGIWKNSNFGKGVIVGVLDTGIHPNHPSFNDEGMSPPPAKWKGKCEFNSSLC 194

Query: 186 NNKLIGARTFNLANNFVIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPL 245
           NNKLIGARTFNL NNF++ +SPNDE GHGTHTASTAAG FV+ AEALGNA+GKA G+APL
Sbjct: 195 NNKLIGARTFNLGNNFLMEESPNDEKGHGTHTASTAAGAFVEDAEALGNAKGKAAGIAPL 254

Query: 246 AHIAMYKVCSPNGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAIGAFAAIKK 305
           AH+A+YKVCS   C SSD+ A +DAAIDDGVDVLS+SLG+ S PFF+D IA+  F AI+K
Sbjct: 255 AHLAIYKVCSGKRCPSSDVFAGIDAAIDDGVDVLSISLGSRSVPFFKDNIAVATFGAIQK 314

Query: 306 GIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNF 365
           GIFVS SAGNSGP  +TL+N+APWILTVGASTI+R+IVA+AKLG+GE Y GESL+QP +F
Sbjct: 315 GIFVSSSAGNSGPLNSTLSNDAPWILTVGASTINRRIVAVAKLGNGEQYEGESLYQPNDF 374

Query: 366 PSKFLPLVYAGKSGIEGSEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAA 425
           PSKFLPLVYAG    +   +C +GSLE ++V GKVVVCE  GG+ RVAKGLVVKN GGAA
Sbjct: 375 PSKFLPLVYAGNRENKTYAFCGEGSLENMDVKGKVVVCEGKGGVGRVAKGLVVKNAGGAA 434

Query: 426 MILINQKPEGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRATT 485
           MILINQ+ +GFSTL+EAHVLP TH+SY+ G+ IK+YINSS NP ASISFKGT++G+    
Sbjct: 435 MILINQEEDGFSTLSEAHVLPATHVSYKAGVLIKSYINSSQNPTASISFKGTVIGDGDDF 494

Query: 486 FSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSM 545
            +P+MASFSSRGPC  SPGILKPDITGPGVNILAAWPFPL+N  +TNTKSTFNVISGTSM
Sbjct: 495 SAPSMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFPLDN--DTNTKSTFNVISGTSM 554

Query: 546 SCPHLSGIAALIKSYHPNWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSGHV 605
           SCPHLSGIAALIKS HPNWSPAAIKSAIMT+A+++ PQG+PI DQDL+PANFFAMG+GHV
Sbjct: 555 SCPHLSGIAALIKSVHPNWSPAAIKSAIMTTANIKTPQGEPITDQDLQPANFFAMGAGHV 614

Query: 606 NPSKAANPGLVYDIQPDDYIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFAV 665
           NPSKAA+PGLVYDIQPDDYIPYLC LY D +VSIIV + V C  V  IREGDLNYPSF+V
Sbjct: 615 NPSKAADPGLVYDIQPDDYIPYLCGLYKDEEVSIIVHRTVICGLVLSIREGDLNYPSFSV 674

Query: 666 SLEAGSQTFNRTVTNVGDANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFSRI 725
           +L  G QTF RTVTNVG+ANSVY  IVEAP GVS+ V P  L FS++N+ +T++VTF+RI
Sbjct: 675 AL-GGLQTFKRTVTNVGEANSVYTAIVEAPLGVSMTVMPRKLFFSRVNQTMTFTVTFNRI 734

Query: 726 DFVRTTSEFSEGYLIWV--SKKLMVRSPISVK 753
            +V+   EF EGYL WV  SKK +VRSP+SVK
Sbjct: 735 RWVKIVGEFGEGYLKWVSKSKKYVVRSPVSVK 762

BLAST of Cmc09g0257011 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 611.7 bits (1576), Expect = 8.0e-175
Identity = 345/748 (46.12%), Postives = 470/748 (62.83%), Query Frame = 0

Query: 27  STSNTNLQTYIVHVKQPVLEILGDTIDL-QNWYTSFLPETIEASSDEQPRLLYSYRHVMS 86
           S+S+++  TYIVH+ +  +     + DL  NWY S L      S  +   LLY+Y + + 
Sbjct: 23  SSSSSDQGTYIVHMAKSQMP---SSFDLHSNWYDSSL-----RSISDSAELLYTYENAIH 82

Query: 87  GFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNTHFG-LWKNSNFGKGVI 146
           GFS RLT+E+  ++  + G IS +PE    LHTT TP +LGL+ H   L+  +     V+
Sbjct: 83  GFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSDVV 142

Query: 147 IGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCE----FGASICNNKLIGARTFNLANNFVI 206
           +GVLDTG+ P   S++DEG    P+ WKG CE    F AS+CN KLIGAR F       +
Sbjct: 143 VGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTM 202

Query: 207 G--------KSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCS 266
           G        +SP D++GHGTHT+STAAG+ V+GA  LG A G A GMAP A +A+YKVC 
Sbjct: 203 GPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCW 262

Query: 267 PNGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAIGAFAAIKKGIFVSCSAGN 326
             GC SSDILAA+D AI D V+VLS+SLG   + ++RD +AIGAFAA+++GI VSCSAGN
Sbjct: 263 LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGN 322

Query: 327 SGPSKNTLANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYA 386
           +GPS ++L+N APWI TVGA T+DR   ALA LG+G+ +TG SLF+    P K LP +YA
Sbjct: 323 AGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYA 382

Query: 387 GK-SGIEGSEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPE 446
           G  S       C+ G+L    V GK+V+C+RG   +RV KG VVK  GG  MIL N    
Sbjct: 383 GNASNATNGNLCMTGTLIPEKVKGKIVMCDRGIN-ARVQKGDVVKAAGGVGMILANTAAN 442

Query: 447 GFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFS 506
           G   +A+AH+LP T +  + G  I+ Y+ +  NP ASIS  GT++G +    SP +A+FS
Sbjct: 443 GEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKP---SPVVAAFS 502

Query: 507 SRGPCQASPGILKPDITGPGVNILAAWPFPLNNN--TNTNTKSTFNVISGTSMSCPHLSG 566
           SRGP   +P ILKPD+  PGVNILAAW          + + +  FN+ISGTSMSCPH+SG
Sbjct: 503 SRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSG 562

Query: 567 IAALIKSYHPNWSPAAIKSAIMTSADVRNPQGKPIMD-QDLKPANFFAMGSGHVNPSKAA 626
           +AAL+KS HP WSPAAI+SA+MT+A      GKP++D    KP+  F  G+GHV+P+ A 
Sbjct: 563 LAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTAT 622

Query: 627 NPGLVYDIQPDDYIPYLCHL-YTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLE-A 686
           NPGL+YD+  +DY+ +LC L YT  Q+  + R+  TC         DLNYPSFAV+++  
Sbjct: 623 NPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGV 682

Query: 687 GSQTFNRTVTNVGDANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVR 746
           G+  + RTVT+VG A +    +     GV + V P+ L F + NEK +Y+VTF+ +D  +
Sbjct: 683 GAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFT-VDSSK 742

Query: 747 TTSEFSEGYLIWVSKKLMVRSPISVKLT 755
            +   S G + W   K +V SP+++  T
Sbjct: 743 PSGSNSFGSIEWSDGKHVVGSPVAISWT 757

BLAST of Cmc09g0257011 vs. TAIR 10
Match: AT1G04110.1 (Subtilase family protein )

HSP 1 Score: 600.5 bits (1547), Expect = 1.8e-171
Identity = 356/785 (45.35%), Postives = 480/785 (61.15%), Query Frame = 0

Query: 6   FICIFFFLNFHGYVAMKTELSSTSNTNLQTYIVHVKQPVLEILGDTIDLQNWYTSFLPET 65
           F+CI F L            SS+     QTYIV +  P  E         +W+ SFL E 
Sbjct: 7   FLCIIFLLFCS---------SSSEILQKQTYIVQL-HPNSETAKTFASKFDWHLSFLQEA 66

Query: 66  I----EASSDEQPRLLYSYRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHT 125
           +    E   +   RLLYSY   + GF+A+LT+ + + +      ++  P+  L + TT++
Sbjct: 67  VLGVEEEEEEPSSRLLYSYGSAIEGFAAQLTESEAEILRYSPEVVAVRPDHVLQVQTTYS 126

Query: 126 PEYLGLN--THFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCE--- 185
            ++LGL+   + G+W  S FG+G IIGVLDTG+ P  PSF+D GM S P KWKG C+   
Sbjct: 127 YKFLGLDGFGNSGVWSKSRFGQGTIIGVLDTGVWPESPSFDDTGMPSIPRKWKGICQEGE 186

Query: 186 -FGASICNNKLIGARTF--------------NLANNFVIGKSPNDENGHGTHTASTAAGT 245
            F +S CN KLIGAR F              N+   ++   S  D  GHGTHTAST  G+
Sbjct: 187 SFSSSSCNRKLIGARFFIRGHRVANSPEESPNMPREYI---SARDSTGHGTHTASTVGGS 246

Query: 246 FVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSSDILAALDAAIDDGVDVLSLSLG 305
            V  A  LGN  G A GMAP AHIA+YKVC  NGC SSDILAA+D AI D VDVLSLSLG
Sbjct: 247 SVSMANVLGNGAGVARGMAPGAHIAVYKVCWFNGCYSSDILAAIDVAIQDKVDVLSLSLG 306

Query: 306 APSTPFFRDTIAIGAFAAIKKGIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVA 365
               P + DTIAIG F A+++GI V C+AGN+GP ++++AN APW+ T+GA T+DR+  A
Sbjct: 307 GFPIPLYDDTIAIGTFRAMERGISVICAAGNNGPIESSVANTAPWVSTIGAGTLDRRFPA 366

Query: 366 LAKLGSGEVYTGESLFQPRNFPS--KFLPLVYAGKSGIEGSEYCIQGSLEKLNVTGKVVV 425
           + +L +G++  GESL+  +   +  + + ++Y    G +GSE+C++GSL +  + GK+V+
Sbjct: 367 VVRLANGKLLYGESLYPGKGIKNAGREVEVIYV-TGGDKGSEFCLRGSLPREEIRGKMVI 426

Query: 426 CERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAEAHVLPTTHLSYEDGLKIKAYI 485
           C+RG    R  KG  VK  GG AMIL N +        + H+LP T + Y + + +KAY+
Sbjct: 427 CDRGVN-GRSEKGEAVKEAGGVAMILANTEINQEEDSIDVHLLPATLIGYTESVLLKAYV 486

Query: 486 NSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWP 545
           N++  PKA I F GT++G    + +P +A FS+RGP  A+P ILKPD+  PGVNI+AAWP
Sbjct: 487 NATVKPKARIIFGGTVIGR---SRAPEVAQFSARGPSLANPSILKPDMIAPGVNIIAAWP 546

Query: 546 FPLNNN--TNTNTKSTFNVISGTSMSCPHLSGIAALIKSYHPNWSPAAIKSAIMTSADVR 605
             L        + +  F V+SGTSMSCPH+SGI ALI+S +PNWSPAAIKSA+MT+AD+ 
Sbjct: 547 QNLGPTGLPYDSRRVNFTVMSGTSMSCPHVSGITALIRSAYPNWSPAAIKSALMTTADLY 606

Query: 606 NPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYIPYLCHL-YTDAQVSI 665
           + QGK I D + KPA  FA+G+GHVNP KA NPGLVY+IQP DYI YLC L +T + +  
Sbjct: 607 DRQGKAIKDGN-KPAGVFAIGAGHVNPQKAINPGLVYNIQPVDYITYLCTLGFTRSDILA 666

Query: 666 IVRKQVTCSTVPRIREG-DLNYPSFAVSLEAGSQT--FNRTVTNVGDANSVYYPIVEAPA 725
           I  K V+C+ + R   G  LNYPS AV  + G  T    R VTNVG  NS+Y   V+AP 
Sbjct: 667 ITHKNVSCNGILRKNPGFSLNYPSIAVIFKRGKTTEMITRRVTNVGSPNSIYSVNVKAPE 726

Query: 726 GVSVKVTPSNLKFSKLNEKVTYSVTF--SRIDFVRTTSEFSEGYLIWV-SKKLM--VRSP 754
           G+ V V P  L F  +++ ++Y V F   + +     + F++G L WV S  LM  VRSP
Sbjct: 727 GIKVIVNPKRLVFKHVDQTLSYRVWFVLKKKNRGGKVASFAQGQLTWVNSHNLMQRVRSP 772

BLAST of Cmc09g0257011 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 589.0 bits (1517), Expect = 5.6e-168
Identity = 349/760 (45.92%), Postives = 461/760 (60.66%), Query Frame = 0

Query: 26  SSTSNTNLQTYIVHVKQPVLEILGDTIDLQNWYTSFLPETIEASSDEQPRLLYSYRHVMS 85
           SS+S+  L++YIVHV++     L  + +  NW+ S L      SS +   LLYSY   + 
Sbjct: 23  SSSSSDGLESYIVHVQRSHKPSLFSSHN--NWHVSLLRSL--PSSPQPATLLYSYSRAVH 82

Query: 86  GFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNTHFGLWKNSNFGKGVII 145
           GFSARL+  Q  A+      IS +P+    +HTTHTP +LG + + GLW NSN+G+ VI+
Sbjct: 83  GFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNSNYGEDVIV 142

Query: 146 GVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFG----ASICNNKLIGARTF--------- 205
           GVLDTGI P HPSF+D G+   P+ WKG CE G    AS CN KLIGAR F         
Sbjct: 143 GVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFYRGYLTQRN 202

Query: 206 -NLANNFVIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVC 265
               +     +SP D  GHGTHTASTAAG+ V  A     ARG A GMA  A IA YK+C
Sbjct: 203 GTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAAYKIC 262

Query: 266 SPNGCSSSDILAALDAAIDDGVDVLSLSLGAPST--PFFRDTIAIGAFAAIKKGIFVSCS 325
              GC  SDILAA+D A+ DGV V+SLS+GA  +   +  D+IAIGAF A + GI VSCS
Sbjct: 263 WTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGIVVSCS 322

Query: 326 AGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPL 385
           AGNSGP+  T  N APWILTVGAST+DR+  A A  G G+V+TG SL+   + P   L L
Sbjct: 323 AGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPDSQLSL 382

Query: 386 VYAGKSGIEGSEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQK 445
           VY+G     GS  C  G L    V GK+V+C+RGG  +RV KG  VK  GGA MIL N  
Sbjct: 383 VYSGDC---GSRLCYPGKLNSSLVEGKIVLCDRGGN-ARVEKGSAVKLAGGAGMILANTA 442

Query: 446 PEGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMAS 505
             G    A++H++P T +  + G +I+ YI +S +P A ISF GTL+G   +  SP +A+
Sbjct: 443 ESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIG--PSPPSPRVAA 502

Query: 506 FSSRGPCQASPGILKPDITGPGVNILAAW-----PFPLNNNTNTNTKSTFNVISGTSMSC 565
           FSSRGP   +P ILKPD+  PGVNILA W     P  L+ +     +  FN+ISGTSMSC
Sbjct: 503 FSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPR---RVQFNIISGTSMSC 562

Query: 566 PHLSGIAALIKSYHPNWSPAAIKSAIMTSA-DVRNPQGKPIMDQDL-KPANFFAMGSGHV 625
           PH+SG+AAL++  HP+WSPAAIKSA++T+A DV N  G+PI D    K +N F  G+GHV
Sbjct: 563 PHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVEN-SGEPIEDLATGKSSNSFIHGAGHV 622

Query: 626 NPSKAANPGLVYDIQPDDYIPYLCHLYTDAQVSIIVRKQVT----CSTVPRIREGDLNYP 685
           +P+KA NPGLVYDI+  +Y+ +LC +  +    ++  +  T    C T      GDLNYP
Sbjct: 623 DPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYP 682

Query: 686 SFAVSLEAGSQT--FNRTVTNVG-DANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTY 745
           SF+V   +  +   + R V NVG + ++VY   V++PA V + V+PS L FSK    + Y
Sbjct: 683 SFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVLEY 742

Query: 746 SVTFSRIDF---VRTTSEFSEGYLIWVSKKLMVRSPISVK 753
            VTF  +     V +      G + W   + +V+SP++V+
Sbjct: 743 EVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQ 768

BLAST of Cmc09g0257011 vs. TAIR 10
Match: AT1G01900.1 (subtilase family protein )

HSP 1 Score: 586.6 bits (1511), Expect = 2.8e-167
Identity = 342/776 (44.07%), Postives = 466/776 (60.05%), Query Frame = 0

Query: 1   MVFLPFICIFFFLNFHGYVAMKTELSSTSNTNLQTYIVHVKQPVLEILGDTIDLQNWYTS 60
           M+F     +FFFL F          +S  ++  QTY++H      + +     + + + S
Sbjct: 18  MMFFRSFIVFFFLIF---------FASNVSSRKQTYVIHTVTTSTKHI-----VTSLFNS 77

Query: 61  FLPETIEASSDEQPRLLYSYRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTH 120
              E I       P + Y Y + MSGFSA LT +Q+  ++   GFISA P+  L+LHTT+
Sbjct: 78  LQTENINDDDFSLPEIHYIYENAMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTY 137

Query: 121 TPEYLGLNTHFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCE---- 180
           + E+LGL    GLW  ++    VIIG++DTGI P H SF D  M+  P++W+G C+    
Sbjct: 138 SHEFLGLEFGIGLWNETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTN 197

Query: 181 FGASICNNKLIGARTFNLANNFVIGK--------SPNDENGHGTHTASTAAGTFVKGAEA 240
           F +S CN K+IGA  F      ++GK        S  D  GHGTHTASTAAG  V  A  
Sbjct: 198 FSSSECNKKIIGASAFYKGYESIVGKINETTDFRSTRDAQGHGTHTASTAAGDIVPKANY 257

Query: 241 LGNARGKAVGMAPLAHIAMYKVCSPNGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFF 300
            G A+G A GM   + IA YK C   GC+S+D++AA+D AI DGVDV+SLSLG  S PF+
Sbjct: 258 FGQAKGLASGMRFTSRIAAYKACWALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPFY 317

Query: 301 RDTIAIGAFAAIKKGIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLGSG 360
            D IAI  F A++K IFVSCSAGNSGP+ +T++N APW++TV AS  DR   A+ ++G+ 
Sbjct: 318 VDPIAIAGFGAMQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNR 377

Query: 361 EVYTGESLFQPRNFPSKFLPLVYAGKSGIE-GSEYCIQGSLEKLNVTGKVVVCERGGGIS 420
           +   G SL++ ++   K LPL +   +G E G+ +CI+ SL++  V GK+V+C RG    
Sbjct: 378 KSLVGSSLYKGKSL--KNLPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGAS-G 437

Query: 421 RVAKGLVVKNGGGAAMILINQKPEGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKA 480
           R AKG  VK  GGAAM+L++ + EG   LA+ HVLP   L + DG  +  Y+  + N  A
Sbjct: 438 RTAKGEEVKRSGGAAMLLVSTEAEGEELLADPHVLPAVSLGFSDGKTLLNYLAGAANATA 497

Query: 481 SISFKGTLLGNRATTFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAW-PFPLNNNT 540
           S+ F+GT  G  A    P +A+FSSRGP  A P I KPDI  PG+NILA W PF   +  
Sbjct: 498 SVRFRGTAYGATA----PMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSLL 557

Query: 541 NTNTKST-FNVISGTSMSCPHLSGIAALIKSYHPNWSPAAIKSAIMTSADVRNPQGKPIM 600
            ++ +   FN+ISGTSM+CPH+SGIAALIKS H +WSPA IKSAIMT+A + + + +PI 
Sbjct: 558 RSDPRRVQFNIISGTSMACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIG 617

Query: 601 DQDL----KPANFFAMGSGHVNPSKAANPGLVYDIQPDDYIPYLCHL-YTDAQVSIIVRK 660
           D+        A  FA G+G+V+P++A +PGLVYD    DY+ YLC L YT  ++ +    
Sbjct: 618 DRGAAGAESAATAFAFGAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGT 677

Query: 661 QVTC-STVPRIREGDLNYPSFAVSLEAGSQ----TFNRTVTNVGDANSVYYPIVEAPAGV 720
             TC S    +  GDLNYPSFAV+L  G+      + RTVTNVG     Y   VE P GV
Sbjct: 678 NYTCASNAVVLSPGDLNYPSFAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKGV 737

Query: 721 SVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIWVSKKLMVRSPISV 752
            V+V P  LKF K  E+++Y+VT+   +  R +S  S G L+W+  K  VRSPI+V
Sbjct: 738 KVRVEPKVLKFQKARERLSYTVTYD-AEASRNSSSSSFGVLVWICDKYNVRSPIAV 771

BLAST of Cmc09g0257011 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 583.2 bits (1502), Expect = 3.0e-166
Identity = 340/779 (43.65%), Postives = 472/779 (60.59%), Query Frame = 0

Query: 11  FFLNFHGYVAMKTELSSTSNTNLQTYIVHVKQPVLEILGDTIDLQNWYTSFLPETIEASS 70
           FF  F   + + +  SS S++N  TYIVHV       +  T    +WYTS    ++ + +
Sbjct: 3   FFFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPT--HFHWYTS----SLASLT 62

Query: 71  DEQPRLLYSYRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNT- 130
              P ++++Y  V  GFSARLT +    + +    IS +PE   +LHTT +PE+LGL + 
Sbjct: 63  SSPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRST 122

Query: 131 -HFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRC----EFGASICNN 190
              GL + S+FG  ++IGV+DTG+ P  PSF+D G+   P KWKG+C    +F  S CN 
Sbjct: 123 DKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNR 182

Query: 191 KLIGARTF--------NLANNFVIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKA 250
           KL+GAR F           N     +SP D +GHGTHTAS +AG +V  A  LG A G A
Sbjct: 183 KLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVA 242

Query: 251 VGMAPLAHIAMYKVCSPNGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAIGA 310
            GMAP A +A YKVC  +GC  SDILAA D A+ DGVDV+SLS+G    P++ D IAIGA
Sbjct: 243 AGMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGA 302

Query: 311 FAAIKKGIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESL 370
           F AI +GIFVS SAGN GP   T+ N APW+ TVGA TIDR   A  KLG+G++ +G S+
Sbjct: 303 FGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSV 362

Query: 371 F-QPRNFPSKFLPLVYAGK-SGIEG--SEYCIQGSLEKLNVTGKVVVCERGGGISRVAKG 430
           +  P   P +  PLVY G   G +G  S  C++GSL+   V GK+V+C+RG   SR  KG
Sbjct: 363 YGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGIN-SRATKG 422

Query: 431 LVVKNGGGAAMILINQKPEGFSTLAEAHVLPTTHLSYEDGLKIKAYIN------SSHNPK 490
            +V+  GG  MI+ N   +G   +A+ HVLP T +    G +I+ YI+      SS +P 
Sbjct: 423 EIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPT 482

Query: 491 ASISFKGTLLGNRATTFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNN- 550
           A+I FKGT LG R    +P +ASFS+RGP   +P ILKPD+  PG+NILAAWP  +  + 
Sbjct: 483 ATIVFKGTRLGIRP---APVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSG 542

Query: 551 -TNTNTKSTFNVISGTSMSCPHLSGIAALIKSYHPNWSPAAIKSAIMTSADVRNPQGKPI 610
            T+ N ++ FN++SGTSM+CPH+SG+AAL+K+ HP+WSPAAI+SA++T+A   +  G+P+
Sbjct: 543 VTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPM 602

Query: 611 MDQDL-KPANFFAMGSGHVNPSKAANPGLVYDIQPDDYIPYLCHL-YTDAQVSIIVRKQV 670
           MD+     ++    GSGHV+P+KA +PGLVYDI   DYI +LC+  YT   +  I R+Q 
Sbjct: 603 MDESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQA 662

Query: 671 TCSTVPRI-REGDLNYPSFAVSLEAGSQT-----FNRTVTNVGDANSVYYPIVEAPAGVS 730
            C    R    G+LNYPSF+V  +   ++     F RTVTNVGD++SVY   +  P G +
Sbjct: 663 DCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTT 722

Query: 731 VKVTPSNLKFSKLNEKVTY--SVTFSRIDFVRTTSEFSEGYLIWVSKKLMVRSPISVKL 754
           V V P  L F ++ +K+++   V  + +      +    G+++W   K  V SP+ V L
Sbjct: 723 VTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTL 771

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008437177.10.0e+0099.60PREDICTED: subtilisin-like protease SBT1.7 [Cucumis melo][more]
KAA0042822.10.0e+00100.00subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa][more]
XP_004147599.20.0e+0093.77subtilisin-like protease SBT1.7 [Cucumis sativus] >KAE8647878.1 hypothetical pro... [more]
XP_038906452.10.0e+0083.65subtilisin-like protease 4 [Benincasa hispida][more]
KAA0042821.10.0e+0071.73subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
A9QY384.8e-25760.76Subtilisin-like protease 4 OS=Lotus japonicus OX=34305 GN=SBTM4 PE=2 SV=1[more]
A0A0M3R8G23.7e-24158.83Subtilisin-like protease OS=Petunia hybrida OX=4102 GN=SBT PE=2 SV=1[more]
A9QY392.3e-23558.14Subtilisin-like protease 3 OS=Lotus japonicus OX=34305 GN=SBTM3 PE=2 SV=1[more]
A9QY402.0e-23158.46Subtilisin-like protease 1 OS=Lotus japonicus OX=34305 GN=SBTM1 PE=2 SV=1[more]
G7KEU72.2e-22556.87Subtilisin-like protease OS=Medicago truncatula OX=3880 GN=SBT PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3AT160.0e+0099.60subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103482683 PE=3 SV=... [more]
A0A5A7THE40.0e+00100.00Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A0A0KMQ70.0e+0093.60Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157280 PE=3 SV=1[more]
A0A5A7TMM40.0e+0071.73Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A1S3AUP12.8e-31071.54subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103483066 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT5G67360.18.0e-17546.12Subtilase family protein [more]
AT1G04110.11.8e-17145.35Subtilase family protein [more]
AT3G14067.15.6e-16845.92Subtilase family protein [more]
AT1G01900.12.8e-16744.07subtilase family protein [more]
AT3G14240.13.0e-16643.65Subtilase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 140..159
score: 44.89
coord: 538..554
score: 56.17
coord: 206..219
score: 54.19
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 35..117
e-value: 5.6E-12
score: 46.2
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 18..117
e-value: 4.4E-24
score: 86.7
NoneNo IPR availableGENE3D3.50.30.30coord: 336..476
e-value: 2.6E-178
score: 595.5
NoneNo IPR availableGENE3D2.60.40.2310coord: 625..753
e-value: 3.4E-37
score: 129.1
NoneNo IPR availablePANTHERPTHR10795:SF697SUBTILISIN-LIKE PROTEASE SBT1.7coord: 30..751
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 124..608
score: 34.637886
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 344..469
e-value: 3.38353E-38
score: 136.389
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 654..751
e-value: 4.7E-28
score: 97.3
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 142..621
e-value: 2.6E-178
score: 595.5
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 116..612
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 140..577
e-value: 4.9E-53
score: 180.6
IPR003137PA domainPFAMPF02225PAcoord: 368..455
e-value: 1.5E-10
score: 41.0
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 30..751
IPR023827Peptidase S8, subtilisin, Asp-active sitePROSITEPS00136SUBTILASE_ASPcoord: 145..156
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 122..575
e-value: 4.81137E-125
score: 373.857

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc09g0257011.1Cmc09g0257011.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0009610 response to symbiotic fungus
cellular_component GO:0016020 membrane
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity