Cmc08g0228281 (gene) Melon (Charmono) v1.1

Overview


Name	Cmc08g0228281
Type	gene
Organism	Cucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Description	Reverse transcriptase
Location	CMiso1.1chr08: 22036818 .. 22037474 (+)
RNA-Seq Expression	Cmc08g0228281
Synteny	Cmc08g0228281

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGGTTGTGCATCGATTATCGACAATTGAACAAGGTAACAATTAAGAATAAGTATACTTTATCCCGAATAGATGACCTCTTTGACCAGTTGAAAGGAGCGTCAGTATTCTCCAAGATAGACTTGTGTTCAGGCTATTGCCAATTAAGAATTAAGAAGTTAAACGTGCCTAAGATAACCTGCAGAACACATTATGGGCATTACAAGTTCTTGGTAATGCCTTTTGGGTTGACCAATGCCCCAACAACCTTTATGGACCTAATGAACCGAGTGTTTCATCACTACTTAGATCAATTTGCCATTGTTTTCAATGATGATATTTTAGTCTACTTCTTAGACGTGAAAAGACATGCGGAACACTTAAGAATTATATTACAAACCGTGCGAGAAGAAAAGCTATTCGCTAAGTTCAGCAAGTGTGAGTTCTGGCTTAACCAAGTAGTGTTTCTTGGGCACATTATATCTTCAGAAGGGTTGAAAGTGGACTCACAAAAGGTAGTAGCAATTGAAACTTGGAATGGCACGAAAACGATTTTTGAAGTTCGGAGCTTTCTCGGCTTGACGGGTTACTATATGCAATTTGTGGAAGGATTTTTCAAATTAGCACTTCCTTTGACAACTCTTACAAGGAAAGAAACAAAATTTGAATGGTCGTAA

mRNA sequence

ATGAGGTTGTGCATCGATTATCGACAATTGAACAAGGTAACAATTAAGAATAAGTATACTTTATCCCGAATAGATGACCTCTTTGACCAGTTGAAAGGAGCGTCAGTATTCTCCAAGATAGACTTGTGTTCAGGCTATTGCCAATTAAGAATTAAGAAGTTAAACGTGCCTAAGATAACCTGCAGAACACATTATGGGCATTACAAGTTCTTGGTAATGCCTTTTGGGTTGACCAATGCCCCAACAACCTTTATGGACCTAATGAACCGAGTGTTTCATCACTACTTAGATCAATTTGCCATTGTTTTCAATGATGATATTTTAGTCTACTTCTTAGACGTGAAAAGACATGCGGAACACTTAAGAATTATATTACAAACCGTGCGAGAAGAAAAGCTATTCGCTAAGTTCAGCAAGTGTGAGTTCTGGCTTAACCAAGTAGTGTTTCTTGGGCACATTATATCTTCAGAAGGGTTGAAAGTGGACTCACAAAAGGTAGTAGCAATTGAAACTTGGAATGGCACGAAAACGATTTTTGAAGTTCGGAGCTTTCTCGGCTTGACGGGTTACTATATGCAATTTGTGGAAGGATTTTTCAAATTAGCACTTCCTTTGACAACTCTTACAAGGAAAGAAACAAAATTTGAATGGTCGTAA

Coding sequence (CDS)

ATGAGGTTGTGCATCGATTATCGACAATTGAACAAGGTAACAATTAAGAATAAGTATACTTTATCCCGAATAGATGACCTCTTTGACCAGTTGAAAGGAGCGTCAGTATTCTCCAAGATAGACTTGTGTTCAGGCTATTGCCAATTAAGAATTAAGAAGTTAAACGTGCCTAAGATAACCTGCAGAACACATTATGGGCATTACAAGTTCTTGGTAATGCCTTTTGGGTTGACCAATGCCCCAACAACCTTTATGGACCTAATGAACCGAGTGTTTCATCACTACTTAGATCAATTTGCCATTGTTTTCAATGATGATATTTTAGTCTACTTCTTAGACGTGAAAAGACATGCGGAACACTTAAGAATTATATTACAAACCGTGCGAGAAGAAAAGCTATTCGCTAAGTTCAGCAAGTGTGAGTTCTGGCTTAACCAAGTAGTGTTTCTTGGGCACATTATATCTTCAGAAGGGTTGAAAGTGGACTCACAAAAGGTAGTAGCAATTGAAACTTGGAATGGCACGAAAACGATTTTTGAAGTTCGGAGCTTTCTCGGCTTGACGGGTTACTATATGCAATTTGTGGAAGGATTTTTCAAATTAGCACTTCCTTTGACAACTCTTACAAGGAAAGAAACAAAATTTGAATGGTCGTAA

Protein sequence

MRLCIDYRQLNKVTIKNKYTLSRIDDLFDQLKGASVFSKIDLCSGYCQLRIKKLNVPKITCRTHYGHYKFLVMPFGLTNAPTTFMDLMNRVFHHYLDQFAIVFNDDILVYFLDVKRHAEHLRIILQTVREEKLFAKFSKCEFWLNQVVFLGHIISSEGLKVDSQKVVAIETWNGTKTIFEVRSFLGLTGYYMQFVEGFFKLALPLTTLTRKETKFEWS

Homology

[Close]

BLAST of Cmc08g0228281 vs. NCBI nr
Match: KAA0037581.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 317.8 bits (813), Expect = 7.4e-83
Identity = 150/217 (69.12%), Postives = 176/217 (81.11%), Query Frame = 0


Query: 1    MRLCIDYRQLNKVTIKNKYTLSRIDDLFDQLKGASVFSKIDLCSGYCQLRIKKLNVPKIT 60
            +RLCIDYRQLNKVTI+NKY L RIDDLFDQL+GA VFSKIDL SGY QL+++K N+PK  
Sbjct: 881  LRLCIDYRQLNKVTIRNKYPLPRIDDLFDQLRGAIVFSKIDLRSGYHQLKVRKSNIPKTA 940

Query: 61   CRTHYGHYKFLVMPFGLTNAPTTFMDLMNRVFHHYLDQFAIVFNDDILVYFLDVKRHAEH 120
             RT YGHY+FLVMPFGLTNAP  FMDL+NR+FH YLDQF IVF DDILVY +D K H EH
Sbjct: 941  FRTRYGHYEFLVMPFGLTNAPAVFMDLINRIFHQYLDQFVIVFIDDILVYSMDKKAHEEH 1000

Query: 121  LRIILQTVREEKLFAKFSKCEFWLNQVVFLGHIISSEGLKVDSQKVVAIETWNGTKTIFE 180
            LRI+LQ +R+++L+ KFSKCEFWLNQVVFLGH++S+ G+ VD QKV  +  W    +  E
Sbjct: 1001 LRIVLQILRDKQLYVKFSKCEFWLNQVVFLGHVVSAGGVSVDPQKVEVVVNWERPASATE 1060

Query: 181  VRSFLGLTGYYMQFVEGFFKLALPLTTLTRKETKFEW 218
            VRSFLGL GYY +FVE F +LALPLT LTRK  +FEW
Sbjct: 1061 VRSFLGLAGYYRRFVEDFSQLALPLTALTRKNAEFEW 1097

[Close]

BLAST of Cmc08g0228281 vs. NCBI nr
Match: TYK03091.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 317.8 bits (813), Expect = 7.4e-83
Identity = 150/217 (69.12%), Postives = 176/217 (81.11%), Query Frame = 0


Query: 1    MRLCIDYRQLNKVTIKNKYTLSRIDDLFDQLKGASVFSKIDLCSGYCQLRIKKLNVPKIT 60
            +RLCIDYRQLNKVTI+NKY L RIDDLFDQL+GA VFSKIDL SGY QL+++K N+PK  
Sbjct: 881  LRLCIDYRQLNKVTIRNKYPLPRIDDLFDQLRGAIVFSKIDLRSGYHQLKVRKSNIPKTA 940

Query: 61   CRTHYGHYKFLVMPFGLTNAPTTFMDLMNRVFHHYLDQFAIVFNDDILVYFLDVKRHAEH 120
             RT YGHY+FLVMPFGLTNAP  FMDL+NR+FH YLDQF IVF DDILVY +D K H EH
Sbjct: 941  FRTRYGHYEFLVMPFGLTNAPAVFMDLINRIFHQYLDQFVIVFIDDILVYSMDKKAHEEH 1000

Query: 121  LRIILQTVREEKLFAKFSKCEFWLNQVVFLGHIISSEGLKVDSQKVVAIETWNGTKTIFE 180
            LRI+LQ +R+++L+ KFSKCEFWLNQVVFLGH++S+ G+ VD QKV  +  W    +  E
Sbjct: 1001 LRIVLQILRDKQLYVKFSKCEFWLNQVVFLGHVVSAGGVSVDPQKVEVVVNWERPASATE 1060

Query: 181  VRSFLGLTGYYMQFVEGFFKLALPLTTLTRKETKFEW 218
            VRSFLGL GYY +FVE F +LALPLT LTRK  +FEW
Sbjct: 1061 VRSFLGLAGYYRRFVEDFSQLALPLTALTRKNAEFEW 1097

[Close]

BLAST of Cmc08g0228281 vs. NCBI nr
Match: TYK05031.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 317.0 bits (811), Expect = 1.3e-82
Identity = 149/218 (68.35%), Postives = 178/218 (81.65%), Query Frame = 0


Query: 1   MRLCIDYRQLNKVTIKNKYTLSRIDDLFDQLKGASVFSKIDLCSGYCQLRIKKLNVPKIT 60
           +RLCIDYRQLNKVTI+NKY L RIDDLFDQLKGA+VFSKIDL  GY QL++++ ++PK  
Sbjct: 182 LRLCIDYRQLNKVTIRNKYPLPRIDDLFDQLKGAAVFSKIDLRLGYYQLKVRESDIPKTA 241

Query: 61  CRTHYGHYKFLVMPFGLTNAPTTFMDLMNRVFHHYLDQFAIVFNDDILVYFLDVKRHAEH 120
            RT YGHY+FLVMPFGLTNAP  FM+LMNR+FH YLDQF IVF DDILVY +D K H EH
Sbjct: 242 FRTRYGHYEFLVMPFGLTNAPENFMNLMNRIFHQYLDQFVIVFIDDILVYSMDKKAHEEH 301

Query: 121 LRIILQTVREEKLFAKFSKCEFWLNQVVFLGHIISSEGLKVDSQKVVAIETWNGTKTIFE 180
           LRI+LQT+R+++L+AKFSKCE WLNQVVFLGH++S++G+ VD QKV  +  W    +  E
Sbjct: 302 LRIVLQTLRDKQLYAKFSKCELWLNQVVFLGHVVSADGVSVDPQKVEGVANWERPVSATE 361

Query: 181 VRSFLGLTGYYMQFVEGFFKLALPLTTLTRKETKFEWS 219
           V SFLGL GYY +F+E F +LALPLT LTRK  KFEWS
Sbjct: 362 VCSFLGLAGYYRRFIEDFSRLALPLTALTRKNAKFEWS 399

[Close]

BLAST of Cmc08g0228281 vs. NCBI nr
Match: KAA0045363.1 (DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 316.2 bits (809), Expect = 2.2e-82
Identity = 149/218 (68.35%), Postives = 180/218 (82.57%), Query Frame = 0


Query: 1   MRLCIDYRQLNKVTIKNKYTLSRIDDLFDQLKGASVFSKIDLCSGYCQLRIKKLNVPKIT 60
           +RLCIDYRQLNKVTI+NKY L RIDDLFDQL+GA++FSKIDL SGY QL++++ ++ K +
Sbjct: 669 LRLCIDYRQLNKVTIRNKYPLPRIDDLFDQLRGAALFSKIDLRSGYHQLKVRESDIAKTS 728

Query: 61  CRTHYGHYKFLVMPFGLTNAPTTFMDLMNRVFHHYLDQFAIVFNDDILVYFLDVKRHAEH 120
            RT YGHY+F VMPFGLTNAP  FMDLMNR+FH YLDQF IVF DDILVY +D + H EH
Sbjct: 729 FRTRYGHYEFRVMPFGLTNAPAVFMDLMNRIFHRYLDQFVIVFIDDILVYSVDRESHEEH 788

Query: 121 LRIILQTVREEKLFAKFSKCEFWLNQVVFLGHIISSEGLKVDSQKVVAIETWNGTKTIFE 180
           LRI+LQT+RE++L+AKFSKCEFWL QVVFLGH++S++G+ VD QKV A+  W    +  E
Sbjct: 789 LRIVLQTLREKQLYAKFSKCEFWLEQVVFLGHVVSAKGVSVDPQKVEAVVNWERPISATE 848

Query: 181 VRSFLGLTGYYMQFVEGFFKLALPLTTLTRKETKFEWS 219
           VRSFLGL GYY +F+E F +LALPLT LTRK  KFEWS
Sbjct: 849 VRSFLGLAGYYRRFIEDFSRLALPLTALTRKNVKFEWS 886

[Close]

BLAST of Cmc08g0228281 vs. NCBI nr
Match: KAA0060440.1 (retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 316.2 bits (809), Expect = 2.2e-82
Identity = 150/218 (68.81%), Postives = 179/218 (82.11%), Query Frame = 0


Query: 1   MRLCIDYRQLNKVTIKNKYTLSRIDDLFDQLKGASVFSKIDLCSGYCQLRIKKLNVPKIT 60
           +RLCIDYRQLNKVTI+NKY L RIDDLFDQL+GA++FSKIDL SGY QL++++ ++ K  
Sbjct: 579 LRLCIDYRQLNKVTIRNKYPLPRIDDLFDQLRGAALFSKIDLRSGYHQLKVRESDIAKTA 638

Query: 61  CRTHYGHYKFLVMPFGLTNAPTTFMDLMNRVFHHYLDQFAIVFNDDILVYFLDVKRHAEH 120
            RT YGHY+F VMPFGLTNAP  FMDLMNR+FH YLDQF IVF DDILVY +D + H EH
Sbjct: 639 FRTRYGHYEFRVMPFGLTNAPAVFMDLMNRIFHRYLDQFVIVFIDDILVYSVDRESHEEH 698

Query: 121 LRIILQTVREEKLFAKFSKCEFWLNQVVFLGHIISSEGLKVDSQKVVAIETWNGTKTIFE 180
           LRI+LQT+RE++L+AKFSKCEFWL QVVFLGH++S++G+ VD QKV AI  W    +  E
Sbjct: 699 LRIVLQTLREKQLYAKFSKCEFWLEQVVFLGHVVSAKGVSVDPQKVEAIVNWGRPISATE 758

Query: 181 VRSFLGLTGYYMQFVEGFFKLALPLTTLTRKETKFEWS 219
           VRSFLGL GYY +F+E F +LALPLT LTRK  KFEWS
Sbjct: 759 VRSFLGLAGYYRRFIEDFSRLALPLTALTRKNVKFEWS 796

[Close]

BLAST of Cmc08g0228281 vs. ExPASy Swiss-Prot
Match: P20825 (Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 1.5e-38
Identity = 75/215 (34.88%), Postives = 129/215 (60.00%), Query Frame = 0


Query: 2   RLCIDYRQLNKVTIKNKYTLSRIDDLFDQLKGASVFSKIDLCSGYCQLRIKKLNVPKITC 61
           R+ IDYR+LN++TI ++Y +  +D++  +L     F+ IDL  G+ Q+ + + ++ K   
Sbjct: 262 RVVIDYRKLNEITIPDRYPIPNMDEILGKLGKCQYFTTIDLAKGFHQIEMDEESISKTAF 321

Query: 62  RTHYGHYKFLVMPFGLTNAPTTFMDLMNRVFHHYLDQFAIVFNDDILVYFLDVKRHAEHL 121
            T  GHY++L MPFGL NAP TF   MN +    L++  +V+ DDI+++   +  H   +
Sbjct: 322 STKSGHYEYLRMPFGLRNAPATFQRCMNNILRPLLNKHCLVYLDDIIIFSTSLTEHLNSI 381

Query: 122 RIILQTVREEKLFAKFSKCEFWLNQVVFLGHIISSEGLKVDSQKVVAIETWNGTKTIFEV 181
           +++   + +  L  +  KCEF   +  FLGHI++ +G+K +  KV AI ++       E+
Sbjct: 382 QLVFTKLADANLKLQLDKCEFLKKEANFLGHIVTPDGIKPNPIKVKAIVSYPIPTKDKEI 441

Query: 182 RSFLGLTGYYMQFVEGFFKLALPLTTLTRKETKFE 217
           R+FLGLTGYY +F+  +  +A P+T+  +K TK +
Sbjct: 442 RAFLGLTGYYRKFIPNYADIAKPMTSCLKKRTKID 476

[Close]

BLAST of Cmc08g0228281 vs. ExPASy Swiss-Prot
Match: P04323 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 2.0e-38
Identity = 73/215 (33.95%), Postives = 130/215 (60.47%), Query Frame = 0


Query: 2   RLCIDYRQLNKVTIKNKYTLSRIDDLFDQLKGASVFSKIDLCSGYCQLRIKKLNVPKITC 61
           R+ IDYR+LN++T+ +++ +  +D++  +L   + F+ IDL  G+ Q+ +   +V K   
Sbjct: 263 RIVIDYRKLNEITVGDRHPIPNMDEILGKLGRCNYFTTIDLAKGFHQIEMDPESVSKTAF 322

Query: 62  RTHYGHYKFLVMPFGLTNAPTTFMDLMNRVFHHYLDQFAIVFNDDILVYFLDVKRHAEHL 121
            T +GHY++L MPFGL NAP TF   MN +    L++  +V+ DDI+V+   +  H + L
Sbjct: 323 STKHGHYEYLRMPFGLKNAPATFQRCMNDILRPLLNKHCLVYLDDIIVFSTSLDEHLQSL 382

Query: 122 RIILQTVREEKLFAKFSKCEFWLNQVVFLGHIISSEGLKVDSQKVVAIETWNGTKTIFEV 181
            ++ + + +  L  +  KCEF   +  FLGH+++ +G+K + +K+ AI+ +       E+
Sbjct: 383 GLVFEKLAKANLKLQLDKCEFLKQETTFLGHVLTPDGIKPNPEKIEAIQKYPIPTKPKEI 442

Query: 182 RSFLGLTGYYMQFVEGFFKLALPLTTLTRKETKFE 217
           ++FLGLTGYY +F+  F  +A P+T   +K  K +
Sbjct: 443 KAFLGLTGYYRKFIPNFADIAKPMTKCLKKNMKID 477

[Close]

BLAST of Cmc08g0228281 vs. ExPASy Swiss-Prot
Match: Q8I7P9 (Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 4.1e-36
Identity = 75/209 (35.89%), Postives = 127/209 (60.77%), Query Frame = 0


Query: 2   RLCIDYRQLNKVTIKNKYTLSRIDDLFDQLKGASVFSKIDLCSGYCQLRIKKLNVPKITC 61
           R+ +D+++LN VTI + Y +  I+     L  A  F+ +DL SG+ Q+ +K+ ++PK   
Sbjct: 179 RMVVDFKRLNTVTIPDTYPIPDINATLASLGNAKYFTTLDLTSGFHQIHMKESDIPKTAF 238

Query: 62  RTHYGHYKFLVMPFGLTNAPTTFMDLMNRVFHHYLDQFAIVFNDDILVYFLDVKRHAEHL 121
            T  G Y+FL +PFGL NAP  F  +++ +   ++ +   V+ DDI+V+  D   H ++L
Sbjct: 239 STLNGKYEFLRLPFGLKNAPAIFQRMIDDILREHIGKVCYVYIDDIIVFSEDYDTHWKNL 298

Query: 122 RIILQTVREEKLFAKFSKCEFWLNQVVFLGHIISSEGLKVDSQKVVAIETWNGTKTIFEV 181
           R++L ++ +  L     K  F   QV FLG+I++++G+K D +KV AI       ++ E+
Sbjct: 299 RLVLASLSKANLQVNLEKSHFLDTQVEFLGYIVTADGIKADPKKVRAISEMPPPTSVKEL 358

Query: 182 RSFLGLTGYYMQFVEGFFKLALPLTTLTR 211
           + FLG+T YY +F++ + K+A PLT LTR
Sbjct: 359 KRFLGMTSYYRKFIQDYAKVAKPLTNLTR 387

[Close]

BLAST of Cmc08g0228281 vs. ExPASy Swiss-Prot
Match: P31843 (RNA-directed DNA polymerase homolog OS=Oenothera berteroana OX=3950 PE=4 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 1.7e-34
Identity = 70/130 (53.85%), Postives = 95/130 (73.08%), Query Frame = 0


Query: 1   MRLCIDYRQLNKVTIKNKYTLSRIDDLFDQLKGASVFSKIDLCSGYCQLRIKKLNVPKIT 60
           +R+CIDYR L KVTIKNKY + R+DDLFD+L  A+ F+K+DL SGY Q+RI K + PK T
Sbjct: 6   LRMCIDYRALTKVTIKNKYPIPRVDDLFDRLAQATWFTKLDLRSGYWQVRIAKGDEPKTT 65

Query: 61  CRTHYGHYKFLVMPFGLTNAPTTFMDLMNRVFHHYLDQFAIVFNDDILVYFL---DVKRH 120
           C T YG ++F VMPFGLTNA  TF +LMN V + YLD F +V+ DD++VY +    +  H
Sbjct: 66  CVTRYGSFEFRVMPFGLTNALATFCNLMNNVLYEYLDHFVVVYLDDLVVYTIYSNSLHEH 125

Query: 121 AEHLRIILQT 128
            +HLR++ ++
Sbjct: 126 IKHLRVVRES 135

[Close]

BLAST of Cmc08g0228281 vs. ExPASy Swiss-Prot
Match: P0CT41 (Transposon Tf2-12 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-12 PE=3 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 2.8e-32
Identity = 67/218 (30.73%), Postives = 122/218 (55.96%), Query Frame = 0


Query: 1   MRLCIDYRQLNKVTIKNKYTLSRIDDLFDQLKGASVFSKIDLCSGYCQLRIKKLNVPKIT 60
           +R+ +DY+ LNK    N Y L  I+ L  +++G+++F+K+DL S Y  +R++K +  K+ 
Sbjct: 462 LRMVVDYKPLNKYVKPNIYPLPLIEQLLAKIQGSTIFTKLDLKSAYHLIRVRKGDEHKLA 521

Query: 61  CRTHYGHYKFLVMPFGLTNAPTTFMDLMNRVFHHYLDQFAIVFNDDILVYFLDVKRHAEH 120
            R   G +++LVMP+G++ AP  F   +N +     +   + + DDIL++      H +H
Sbjct: 522 FRCPRGVFEYLVMPYGISTAPAHFQYFINTILGEAKESHVVCYMDDILIHSKSESEHVKH 581

Query: 121 LRIILQTVREEKLFAKFSKCEFWLNQVVFLGHIISSEGLKVDSQKVVAIETWNGTKTIFE 180
           ++ +LQ ++   L    +KCEF  +QV F+G+ IS +G     + +  +  W   K   E
Sbjct: 582 VKDVLQKLKNANLIINQAKCEFHQSQVKFIGYHISEKGFTPCQENIDKVLQWKQPKNRKE 641

Query: 181 VRSFLGLTGYYMQFVEGFFKLALPLTTLTRKETKFEWS 219
           +R FLG   Y  +F+    +L  PL  L +K+ +++W+
Sbjct: 642 LRQFLGSVNYLRKFIPKTSQLTHPLNNLLKKDVRWKWT 679

[Close]

BLAST of Cmc08g0228281 vs. ExPASy TrEMBL
Match: A0A5D3BTP3 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold374G00020 PE=4 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 3.6e-83
Identity = 150/217 (69.12%), Postives = 176/217 (81.11%), Query Frame = 0


Query: 1    MRLCIDYRQLNKVTIKNKYTLSRIDDLFDQLKGASVFSKIDLCSGYCQLRIKKLNVPKIT 60
            +RLCIDYRQLNKVTI+NKY L RIDDLFDQL+GA VFSKIDL SGY QL+++K N+PK  
Sbjct: 881  LRLCIDYRQLNKVTIRNKYPLPRIDDLFDQLRGAIVFSKIDLRSGYHQLKVRKSNIPKTA 940

Query: 61   CRTHYGHYKFLVMPFGLTNAPTTFMDLMNRVFHHYLDQFAIVFNDDILVYFLDVKRHAEH 120
             RT YGHY+FLVMPFGLTNAP  FMDL+NR+FH YLDQF IVF DDILVY +D K H EH
Sbjct: 941  FRTRYGHYEFLVMPFGLTNAPAVFMDLINRIFHQYLDQFVIVFIDDILVYSMDKKAHEEH 1000

Query: 121  LRIILQTVREEKLFAKFSKCEFWLNQVVFLGHIISSEGLKVDSQKVVAIETWNGTKTIFE 180
            LRI+LQ +R+++L+ KFSKCEFWLNQVVFLGH++S+ G+ VD QKV  +  W    +  E
Sbjct: 1001 LRIVLQILRDKQLYVKFSKCEFWLNQVVFLGHVVSAGGVSVDPQKVEVVVNWERPASATE 1060

Query: 181  VRSFLGLTGYYMQFVEGFFKLALPLTTLTRKETKFEW 218
            VRSFLGL GYY +FVE F +LALPLT LTRK  +FEW
Sbjct: 1061 VRSFLGLAGYYRRFVEDFSQLALPLTALTRKNAEFEW 1097

[Close]

BLAST of Cmc08g0228281 vs. ExPASy TrEMBL
Match: A0A5A7T7M6 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold277G002250 PE=4 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 3.6e-83
Identity = 150/217 (69.12%), Postives = 176/217 (81.11%), Query Frame = 0


Query: 1    MRLCIDYRQLNKVTIKNKYTLSRIDDLFDQLKGASVFSKIDLCSGYCQLRIKKLNVPKIT 60
            +RLCIDYRQLNKVTI+NKY L RIDDLFDQL+GA VFSKIDL SGY QL+++K N+PK  
Sbjct: 881  LRLCIDYRQLNKVTIRNKYPLPRIDDLFDQLRGAIVFSKIDLRSGYHQLKVRKSNIPKTA 940

Query: 61   CRTHYGHYKFLVMPFGLTNAPTTFMDLMNRVFHHYLDQFAIVFNDDILVYFLDVKRHAEH 120
             RT YGHY+FLVMPFGLTNAP  FMDL+NR+FH YLDQF IVF DDILVY +D K H EH
Sbjct: 941  FRTRYGHYEFLVMPFGLTNAPAVFMDLINRIFHQYLDQFVIVFIDDILVYSMDKKAHEEH 1000

Query: 121  LRIILQTVREEKLFAKFSKCEFWLNQVVFLGHIISSEGLKVDSQKVVAIETWNGTKTIFE 180
            LRI+LQ +R+++L+ KFSKCEFWLNQVVFLGH++S+ G+ VD QKV  +  W    +  E
Sbjct: 1001 LRIVLQILRDKQLYVKFSKCEFWLNQVVFLGHVVSAGGVSVDPQKVEVVVNWERPASATE 1060

Query: 181  VRSFLGLTGYYMQFVEGFFKLALPLTTLTRKETKFEW 218
            VRSFLGL GYY +FVE F +LALPLT LTRK  +FEW
Sbjct: 1061 VRSFLGLAGYYRRFVEDFSQLALPLTALTRKNAEFEW 1097

[Close]

BLAST of Cmc08g0228281 vs. ExPASy TrEMBL
Match: A0A5D3BZC5 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G002860 PE=4 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 6.1e-83
Identity = 149/218 (68.35%), Postives = 178/218 (81.65%), Query Frame = 0


Query: 1   MRLCIDYRQLNKVTIKNKYTLSRIDDLFDQLKGASVFSKIDLCSGYCQLRIKKLNVPKIT 60
           +RLCIDYRQLNKVTI+NKY L RIDDLFDQLKGA+VFSKIDL  GY QL++++ ++PK  
Sbjct: 182 LRLCIDYRQLNKVTIRNKYPLPRIDDLFDQLKGAAVFSKIDLRLGYYQLKVRESDIPKTA 241

Query: 61  CRTHYGHYKFLVMPFGLTNAPTTFMDLMNRVFHHYLDQFAIVFNDDILVYFLDVKRHAEH 120
            RT YGHY+FLVMPFGLTNAP  FM+LMNR+FH YLDQF IVF DDILVY +D K H EH
Sbjct: 242 FRTRYGHYEFLVMPFGLTNAPENFMNLMNRIFHQYLDQFVIVFIDDILVYSMDKKAHEEH 301

Query: 121 LRIILQTVREEKLFAKFSKCEFWLNQVVFLGHIISSEGLKVDSQKVVAIETWNGTKTIFE 180
           LRI+LQT+R+++L+AKFSKCE WLNQVVFLGH++S++G+ VD QKV  +  W    +  E
Sbjct: 302 LRIVLQTLRDKQLYAKFSKCELWLNQVVFLGHVVSADGVSVDPQKVEGVANWERPVSATE 361

Query: 181 VRSFLGLTGYYMQFVEGFFKLALPLTTLTRKETKFEWS 219
           V SFLGL GYY +F+E F +LALPLT LTRK  KFEWS
Sbjct: 362 VCSFLGLAGYYRRFIEDFSRLALPLTALTRKNAKFEWS 399

[Close]

BLAST of Cmc08g0228281 vs. ExPASy TrEMBL
Match: A0A5A7TP97 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold316G001420 PE=4 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 1.0e-82
Identity = 149/218 (68.35%), Postives = 180/218 (82.57%), Query Frame = 0


Query: 1   MRLCIDYRQLNKVTIKNKYTLSRIDDLFDQLKGASVFSKIDLCSGYCQLRIKKLNVPKIT 60
           +RLCIDYRQLNKVTI+NKY L RIDDLFDQL+GA++FSKIDL SGY QL++++ ++ K +
Sbjct: 669 LRLCIDYRQLNKVTIRNKYPLPRIDDLFDQLRGAALFSKIDLRSGYHQLKVRESDIAKTS 728

Query: 61  CRTHYGHYKFLVMPFGLTNAPTTFMDLMNRVFHHYLDQFAIVFNDDILVYFLDVKRHAEH 120
            RT YGHY+F VMPFGLTNAP  FMDLMNR+FH YLDQF IVF DDILVY +D + H EH
Sbjct: 729 FRTRYGHYEFRVMPFGLTNAPAVFMDLMNRIFHRYLDQFVIVFIDDILVYSVDRESHEEH 788

Query: 121 LRIILQTVREEKLFAKFSKCEFWLNQVVFLGHIISSEGLKVDSQKVVAIETWNGTKTIFE 180
           LRI+LQT+RE++L+AKFSKCEFWL QVVFLGH++S++G+ VD QKV A+  W    +  E
Sbjct: 789 LRIVLQTLREKQLYAKFSKCEFWLEQVVFLGHVVSAKGVSVDPQKVEAVVNWERPISATE 848

Query: 181 VRSFLGLTGYYMQFVEGFFKLALPLTTLTRKETKFEWS 219
           VRSFLGL GYY +F+E F +LALPLT LTRK  KFEWS
Sbjct: 849 VRSFLGLAGYYRRFIEDFSRLALPLTALTRKNVKFEWS 886

[Close]

BLAST of Cmc08g0228281 vs. ExPASy TrEMBL
Match: A0A5A7UZA9 (Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold22G002660 PE=4 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 1.0e-82
Identity = 150/218 (68.81%), Postives = 179/218 (82.11%), Query Frame = 0


Query: 1   MRLCIDYRQLNKVTIKNKYTLSRIDDLFDQLKGASVFSKIDLCSGYCQLRIKKLNVPKIT 60
           +RLCIDYRQLNKVTI+NKY L RIDDLFDQL+GA++FSKIDL SGY QL++++ ++ K  
Sbjct: 579 LRLCIDYRQLNKVTIRNKYPLPRIDDLFDQLRGAALFSKIDLRSGYHQLKVRESDIAKTA 638

Query: 61  CRTHYGHYKFLVMPFGLTNAPTTFMDLMNRVFHHYLDQFAIVFNDDILVYFLDVKRHAEH 120
            RT YGHY+F VMPFGLTNAP  FMDLMNR+FH YLDQF IVF DDILVY +D + H EH
Sbjct: 639 FRTRYGHYEFRVMPFGLTNAPAVFMDLMNRIFHRYLDQFVIVFIDDILVYSVDRESHEEH 698

Query: 121 LRIILQTVREEKLFAKFSKCEFWLNQVVFLGHIISSEGLKVDSQKVVAIETWNGTKTIFE 180
           LRI+LQT+RE++L+AKFSKCEFWL QVVFLGH++S++G+ VD QKV AI  W    +  E
Sbjct: 699 LRIVLQTLREKQLYAKFSKCEFWLEQVVFLGHVVSAKGVSVDPQKVEAIVNWGRPISATE 758

Query: 181 VRSFLGLTGYYMQFVEGFFKLALPLTTLTRKETKFEWS 219
           VRSFLGL GYY +F+E F +LALPLT LTRK  KFEWS
Sbjct: 759 VRSFLGLAGYYRRFIEDFSRLALPLTALTRKNVKFEWS 796

[Close]

BLAST of Cmc08g0228281 vs. TAIR 10
Match: ATMG00860.1 (DNA/RNA polymerases superfamily protein )

HSP 1 Score: 79.7 bits (195), Expect = 3.2e-15
Identity = 41/101 (40.59%), Postives = 60/101 (59.41%), Query Frame = 0


Query: 120 HLRIILQTVREEKLFAKFSKCEFWLNQVVFLG--HIISSEGLKVDSQKVVAIETWNGTKT 179
           HL ++LQ   + + +A   KC F   Q+ +LG  HIIS EG+  D  K+ A+  W   K 
Sbjct: 3   HLGMVLQIWEQHQFYANRKKCAFGQPQIAYLGHRHIISGEGVSADPAKLEAMVGWPEPKN 62

Query: 180 IFEVRSFLGLTGYYMQFVEGFFKLALPLTTLTRKETKFEWS 219
             E+R FLGLTGYY +FV+ + K+  PLT L +K +  +W+
Sbjct: 63  TTELRGFLGLTGYYRRFVKNYGKIVRPLTELLKKNS-LKWT 102

The following BLAST results are available for this feature:

BLAST of Cmc08g0228281 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0037581.1	7.4e-83	69.12	reverse transcriptase [Cucumis melo var. makuwa]	[more]
TYK03091.1	7.4e-83	69.12	reverse transcriptase [Cucumis melo var. makuwa]	[more]
TYK05031.1	1.3e-82	68.35	reverse transcriptase [Cucumis melo var. makuwa]	[more]
KAA0045363.1	2.2e-82	68.35	DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa]	[more]
KAA0060440.1	2.2e-82	68.81	retrotransposon protein [Cucumis melo var. makuwa]	[more]

BLAST of Cmc08g0228281 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P20825	1.5e-38	34.88	Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaste...	[more]
P04323	2.0e-38	33.95	Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogast...	[more]
Q8I7P9	4.1e-36	35.89	Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogast...	[more]
P31843	1.7e-34	53.85	RNA-directed DNA polymerase homolog OS=Oenothera berteroana OX=3950 PE=4 SV=1	[more]
P0CT41	2.8e-32	30.73	Transposon Tf2-12 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24...	[more]

BLAST of Cmc08g0228281 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BTP3	3.6e-83	69.12	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold37...	[more]
A0A5A7T7M6	3.6e-83	69.12	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold27...	[more]
A0A5D3BZC5	6.1e-83	68.35	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold14...	[more]
A0A5A7TP97	1.0e-82	68.35	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold31...	[more]
A0A5A7UZA9	1.0e-82	68.81	Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]

BLAST of Cmc08g0228281 vs. TAIR 10


Match Name	E-value	Identity	Description
ATMG00860.1	3.2e-15	40.59	DNA/RNA polymerases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000477	Reverse transcriptase domain	PFAM	PF00078	RVT_1	coord: 3..154 e-value: 1.7E-18 score: 67.0
IPR000477	Reverse transcriptase domain	PROSITE	PS50878	RT_POL	coord: 1..154 score: 8.937115
IPR043128	Reverse transcriptase/Diguanylate cyclase domain	GENE3D	3.30.70.270		coord: 163..218 e-value: 1.5E-13 score: 52.5
IPR043128	Reverse transcriptase/Diguanylate cyclase domain	GENE3D	3.30.70.270		coord: 19..154 e-value: 7.7E-56 score: 190.7
None	No IPR available	GENE3D	3.10.10.10	HIV Type 1 Reverse Transcriptase, subunit A, domain 1	coord: 1..79 e-value: 7.7E-56 score: 190.7
None	No IPR available	PANTHER	PTHR24559:SF324	TRANSPOSON TY3-I GAG-POL POLYPROTEIN-LIKE PROTEIN	coord: 2..214
None	No IPR available	PANTHER	PTHR24559	TRANSPOSON TY3-I GAG-POL POLYPROTEIN	coord: 2..214
None	No IPR available	CDD	cd01647	RT_LTR	coord: 2..154 e-value: 3.77171E-65 score: 197.433
IPR043502	DNA/RNA polymerase superfamily	SUPERFAMILY	56672	DNA/RNA polymerases	coord: 1..218

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cmc08g0228281.1	Cmc08g0228281.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
biological_process	GO:0006508	proteolysis
biological_process	GO:0006278	RNA-dependent DNA biosynthetic process
molecular_function	GO:0004190	aspartic-type endopeptidase activity
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0003964	RNA-directed DNA polymerase activity
molecular_function	GO:0008270	zinc ion binding