Cmc08g0222891 (gene) Melon (Charmono) v1.1

Overview
NameCmc08g0222891
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionAuxin efflux carrier component
LocationCMiso1.1chr08: 12000046 .. 12003067 (+)
RNA-Seq ExpressionCmc08g0222891
SyntenyCmc08g0222891
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGAGAGACAGCATAATGTCCTAAAGAATCTCATGAAGCTCCTGAACTCTCACTTTCCCAGTGGTCTCTCAGAATCACTTTTTCTCCCCTTTCTCTCTCTCGATCTCTCTTTCTTCTTCAATTTTCTTTTCCATTCTGATTACACATAAAGTTCTTCAGAACCCCAAAAAAAAAAAATCAGCTTTTTCCTCTCCTTTTTCCCTTCCTTCTGTCCACCATTACAATGCCTCCATAAATACACCTCCCCACCTCCCCTTTTCCTCTTACTTCTTCTTCCACCTCCTCAATGGCTTAAACCCCATAAACAAAACACAGTGTCTCCCCACCCAAAATTCCCAACATGATCTCTGTTTCTGATCTTTATCATGTCCTCACCGCCGTCGTCCCTCTCTACGTCGCCATGATCCTTGCCTACGGCTCCGTCAAATGGTGGAAAATCTTCTCCCCTGACCAATGCTCCGGCATCAACCGTTTCGTCGCACTCTTTGCCGTCCCCCTCCTCTCCTTCCATTTCATTTCCTCCAACAACCCTTTCTCTATGAACTTACGTTTCATCGCCGCGGATACGCTTCAGAAACTTATTGTCCTCGCCGCCCTCGCTGTTTGGTCTCATCTCTCTTCCAAAGGCTCTTTAGAATGGTCGATTACTCTGTTTTCTCTGTCCACTCTGCCTAACACTCTGGTAATGGGAATTCCTCTGTTGAAGGGAATGTACGGCGACTCCACCGGTACTTTAATGGTTCAAATCGTTGTTCTTCAGTGTATCATTTGGTATACATTGATGTTGTTCTTGTTCGAGTACAGGGGGGCTAGATTATTGATTGCAGAGCAGTTTCCGGATACTGCAGGGGAGATTGTTTCCTTTAGAGTTGATTCGGATATTCTGTCTTTAGATGGGAAAGAGCCGTTACAGACGGAGGCGGAGATTGGGGAAGATGGGAAGTTGAGAGTGGTGGTGAGGAAGTCGACGAGTTCTCGATCGGAGGTTTTTTCGCGGCGGTCGCACGGTGGTGGAGGTGGGGTTTCATTAACGCCGCGGCCGTCGAATTTGACTAATGCAGAGATATATTCTTTGCAGTCATCGAGGAACCCAACGCCGAGAGGATCGAGTTTTAATCATTCGGATTTCTTTTTGGGGAATGCGAGTCCGAGGCATTCGAATTTTGGTAATTTTGGGAATTTTGATGAAGAAAATGGAGGACTGAAAAGCAGAGGAATTAGTGGTAATGTCAATGGGATTTTTCCTAGCTCTAATGGGTACCCGGCGCCGGCGAGTGGGGGACTTTTTTCGCCGGTGACCGGACCGGCAGCGACGAAGAAGAGGAATGGCGGTGATGGGGGAAAGGATTTGCATATGTTTGTTTGGAGTTCGAGTGCGTCGCCGGTTTCCGAGGGTGGAATCAACGTTTTCCGGAGTGGAGATTACGACGGACCCGGCGCCGGTGGGATGAACCAGAAAGGTAAAGAGTTGAAAGTTCTATAATTCTTTTTCCCTTTTTTGATTGTTTGGAAGATTTGATTTTATGGGTATTTTGTTGTTTGGTTGAATCTTACTTTTTTCAAGTGGGTCAATAAAGAACTTAAATTTCCTTGTTTTTTTCTCTAGTTTGTCCCCATTTTGTCCAAAAGAGTTTACTTTATTGATTTTTAGGTTATTATTCATATGTTTGAGGTGAATTTTGTTTTGTTAGATTTCAATGAGTATGGTGGGGATGAGTTCAGCTTTGGGAACAAGTCCGCCGTGAACGGCGGCGGACACGACGGAGGCCCTGTGCTATCCAAGCTTGGGTCAAGCTCCACTGCTGAACTCCACCCGAAAAATGGCGATGATACAGCAGAGTCTAAGCCGACCTCAATGCCCCCAGCGAGTGTGATGACAAGGTTGATTTTAATCATGGTTTGGAGGAAGCTGATTAGAAATCCTAATACATATTCAAGCCTGATCGGCCTTGCTTGGTCTTTGATCTCTTTTAGGTACCCTTAAAAGAACCCTAATTTTGGTATTTCGAACTCTAGTTTAGTGGTCAACTAATCATGGCTTTGTGTTTCTTGTAGGTGGAATATTGCAATGCCAGCAATTGTGGCTAGATCGATCGCTATTTTATCCGATGCTGGGCTCGGAATGGCGATGTTTAGTCTCGGTACTGACTTCTTTCTCGTTTGCAAGTCAATTTACTTAGGTTCAGGTTAAATAGTTACATGTTATGGGAATGTGTGTAGGGTTGTTCATGGCATTGCAACCTAAAATCATTGCGTGTGGAAATACCATTGCTTCATTTGCCATGGCGGTCCGGTTCATCACTGGACCGGCGGTTATGGCAGCTGCCTCCATTGCTGTTGGGCTCAGAGGAGTTCTCTTGCATATTGCTATAGTCCAGGTGATGAAAACTAAACACCAAGTTAAATTTGTTCCAAATGACTTTCATTTTTATTATTATGTAAATGAAATTAATGTTTGTTTCATTTGTAGGCTGCCCTACCTCAAGGGATTGTCCCTTTTGTGTTTGCTAAGGAATACAACGTTCATCCTGACATTTTGAGCACCGGGTTCGATCTCGATTTCGACCTCAATTCTAATAAATTCCTATAGTCAATTTATAGCACTTACATGGTTTCTCTTTTTTTTTTTTTATTTTTTTTTATGATGTTTTGCAGAGTTATATTTGGGATGCTGATAGCTCTACCAATAACTTTGGTTTATTACATTTTGTTGGGACTTTGAAGATGCAAAACAGTGGGGAGGGACGAAGATTGGTCTTTTTCTCTCTCTAGAAACACAATTTTTATCTAACTAAAGAAGAAAACGATTTGTTGATGGGGAAAAGAGAGGGAAGAAAAAGTGGAGTTAACTTTTCTTTTTTTGTTTGTTGTAATTTTTTGGCCTCTTTTTGTGCTCAATTTCTTTTGTTCTTTTGATGGGGAAGTAAGTAAATGAAAGCTTTTTAAGATTTGTCTCTCAACTTCAACTACCCTTTTCTCCCCCTAACAAACAAATAAATAAATAAATAAATAATATGTTGA

mRNA sequence

AAAGAGAGACAGCATAATGTCCTAAAGAATCTCATGAAGCTCCTGAACTCTCACTTTCCCAGTGGTCTCTCAGAATCACTTTTTCTCCCCTTTCTCTCTCTCGATCTCTCTTTCTTCTTCAATTTTCTTTTCCATTCTGATTACACATAAAGTTCTTCAGAACCCCAAAAAAAAAAAATCAGCTTTTTCCTCTCCTTTTTCCCTTCCTTCTGTCCACCATTACAATGCCTCCATAAATACACCTCCCCACCTCCCCTTTTCCTCTTACTTCTTCTTCCACCTCCTCAATGGCTTAAACCCCATAAACAAAACACAGTGTCTCCCCACCCAAAATTCCCAACATGATCTCTGTTTCTGATCTTTATCATGTCCTCACCGCCGTCGTCCCTCTCTACGTCGCCATGATCCTTGCCTACGGCTCCGTCAAATGGTGGAAAATCTTCTCCCCTGACCAATGCTCCGGCATCAACCGTTTCGTCGCACTCTTTGCCGTCCCCCTCCTCTCCTTCCATTTCATTTCCTCCAACAACCCTTTCTCTATGAACTTACGTTTCATCGCCGCGGATACGCTTCAGAAACTTATTGTCCTCGCCGCCCTCGCTGTTTGGTCTCATCTCTCTTCCAAAGGCTCTTTAGAATGGTCGATTACTCTGTTTTCTCTGTCCACTCTGCCTAACACTCTGGTAATGGGAATTCCTCTGTTGAAGGGAATGTACGGCGACTCCACCGGTACTTTAATGGTTCAAATCGTTGTTCTTCAGTGTATCATTTGGTATACATTGATGTTGTTCTTGTTCGAGTACAGGGGGGCTAGATTATTGATTGCAGAGCAGTTTCCGGATACTGCAGGGGAGATTGTTTCCTTTAGAGTTGATTCGGATATTCTGTCTTTAGATGGGAAAGAGCCGTTACAGACGGAGGCGGAGATTGGGGAAGATGGGAAGTTGAGAGTGGTGGTGAGGAAGTCGACGAGTTCTCGATCGGAGGTTTTTTCGCGGCGGTCGCACGGTGGTGGAGGTGGGGTTTCATTAACGCCGCGGCCGTCGAATTTGACTAATGCAGAGATATATTCTTTGCAGTCATCGAGGAACCCAACGCCGAGAGGATCGAGTTTTAATCATTCGGATTTCTTTTTGGGGAATGCGAGTCCGAGGCATTCGAATTTTGGTAATTTTGGGAATTTTGATGAAGAAAATGGAGGACTGAAAAGCAGAGGAATTAGTGGTAATGTCAATGGGATTTTTCCTAGCTCTAATGGGTACCCGGCGCCGGCGAGTGGGGGACTTTTTTCGCCGGTGACCGGACCGGCAGCGACGAAGAAGAGGAATGGCGGTGATGGGGGAAAGGATTTGCATATGTTTGTTTGGAGTTCGAGTGCGTCGCCGGTTTCCGAGGGTGGAATCAACGTTTTCCGGAGTGGAGATTACGACGGACCCGGCGCCGGTGGGATGAACCAGAAAGATTTCAATGAGTATGGTGGGGATGAGTTCAGCTTTGGGAACAAGTCCGCCGTGAACGGCGGCGGACACGACGGAGGCCCTGTGCTATCCAAGCTTGGGTCAAGCTCCACTGCTGAACTCCACCCGAAAAATGGCGATGATACAGCAGAGTCTAAGCCGACCTCAATGCCCCCAGCGAGTGTGATGACAAGGTTGATTTTAATCATGGTTTGGAGGAAGCTGATTAGAAATCCTAATACATATTCAAGCCTGATCGGCCTTGCTTGGTCTTTGATCTCTTTTAGGTGGAATATTGCAATGCCAGCAATTGTGGCTAGATCGATCGCTATTTTATCCGATGCTGGGCTCGGAATGGCGATGTTTAGTCTCGGGTTGTTCATGGCATTGCAACCTAAAATCATTGCGTGTGGAAATACCATTGCTTCATTTGCCATGGCGGTCCGGTTCATCACTGGACCGGCGGTTATGGCAGCTGCCTCCATTGCTGTTGGGCTCAGAGGAGTTCTCTTGCATATTGCTATAGTCCAGGCTGCCCTACCTCAAGGGATTGTCCCTTTTGTGTTTGCTAAGGAATACAACGTTCATCCTGACATTTTGAGCACCGGAGTTATATTTGGGATGCTGATAGCTCTACCAATAACTTTGGTTTATTACATTTTGTTGGGACTTTGAAGATGCAAAACAGTGGGGAGGGACGAAGATTGGTCTTTTTCTCTCTCTAGAAACACAATTTTTATCTAACTAAAGAAGAAAACGATTTGTTGATGGGGAAAAGAGAGGGAAGAAAAAGTGGAGTTAACTTTTCTTTTTTTGTTTGTTGTAATTTTTTGGCCTCTTTTTGTGCTCAATTTCTTTTGTTCTTTTGATGGGGAAGTAAGTAAATGAAAGCTTTTTAAGATTTGTCTCTCAACTTCAACTACCCTTTTCTCCCCCTAACAAACAAATAAATAAATAAATAAATAATATGTTGA

Coding sequence (CDS)

ATGATCTCTGTTTCTGATCTTTATCATGTCCTCACCGCCGTCGTCCCTCTCTACGTCGCCATGATCCTTGCCTACGGCTCCGTCAAATGGTGGAAAATCTTCTCCCCTGACCAATGCTCCGGCATCAACCGTTTCGTCGCACTCTTTGCCGTCCCCCTCCTCTCCTTCCATTTCATTTCCTCCAACAACCCTTTCTCTATGAACTTACGTTTCATCGCCGCGGATACGCTTCAGAAACTTATTGTCCTCGCCGCCCTCGCTGTTTGGTCTCATCTCTCTTCCAAAGGCTCTTTAGAATGGTCGATTACTCTGTTTTCTCTGTCCACTCTGCCTAACACTCTGGTAATGGGAATTCCTCTGTTGAAGGGAATGTACGGCGACTCCACCGGTACTTTAATGGTTCAAATCGTTGTTCTTCAGTGTATCATTTGGTATACATTGATGTTGTTCTTGTTCGAGTACAGGGGGGCTAGATTATTGATTGCAGAGCAGTTTCCGGATACTGCAGGGGAGATTGTTTCCTTTAGAGTTGATTCGGATATTCTGTCTTTAGATGGGAAAGAGCCGTTACAGACGGAGGCGGAGATTGGGGAAGATGGGAAGTTGAGAGTGGTGGTGAGGAAGTCGACGAGTTCTCGATCGGAGGTTTTTTCGCGGCGGTCGCACGGTGGTGGAGGTGGGGTTTCATTAACGCCGCGGCCGTCGAATTTGACTAATGCAGAGATATATTCTTTGCAGTCATCGAGGAACCCAACGCCGAGAGGATCGAGTTTTAATCATTCGGATTTCTTTTTGGGGAATGCGAGTCCGAGGCATTCGAATTTTGGTAATTTTGGGAATTTTGATGAAGAAAATGGAGGACTGAAAAGCAGAGGAATTAGTGGTAATGTCAATGGGATTTTTCCTAGCTCTAATGGGTACCCGGCGCCGGCGAGTGGGGGACTTTTTTCGCCGGTGACCGGACCGGCAGCGACGAAGAAGAGGAATGGCGGTGATGGGGGAAAGGATTTGCATATGTTTGTTTGGAGTTCGAGTGCGTCGCCGGTTTCCGAGGGTGGAATCAACGTTTTCCGGAGTGGAGATTACGACGGACCCGGCGCCGGTGGGATGAACCAGAAAGATTTCAATGAGTATGGTGGGGATGAGTTCAGCTTTGGGAACAAGTCCGCCGTGAACGGCGGCGGACACGACGGAGGCCCTGTGCTATCCAAGCTTGGGTCAAGCTCCACTGCTGAACTCCACCCGAAAAATGGCGATGATACAGCAGAGTCTAAGCCGACCTCAATGCCCCCAGCGAGTGTGATGACAAGGTTGATTTTAATCATGGTTTGGAGGAAGCTGATTAGAAATCCTAATACATATTCAAGCCTGATCGGCCTTGCTTGGTCTTTGATCTCTTTTAGGTGGAATATTGCAATGCCAGCAATTGTGGCTAGATCGATCGCTATTTTATCCGATGCTGGGCTCGGAATGGCGATGTTTAGTCTCGGGTTGTTCATGGCATTGCAACCTAAAATCATTGCGTGTGGAAATACCATTGCTTCATTTGCCATGGCGGTCCGGTTCATCACTGGACCGGCGGTTATGGCAGCTGCCTCCATTGCTGTTGGGCTCAGAGGAGTTCTCTTGCATATTGCTATAGTCCAGGCTGCCCTACCTCAAGGGATTGTCCCTTTTGTGTTTGCTAAGGAATACAACGTTCATCCTGACATTTTGAGCACCGGAGTTATATTTGGGATGCTGATAGCTCTACCAATAACTTTGGTTTATTACATTTTGTTGGGACTTTGA

Protein sequence

MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFISSNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPLLKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSDILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGLKSRGISGNVNGIFPSSNGYPAPASGGLFSPVTGPAATKKRNGGDGGKDLHMFVWSSSASPVSEGGINVFRSGDYDGPGAGGMNQKDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKNGDDTAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPAIVARSIAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIAVGLRGVLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL
Homology
BLAST of Cmc08g0222891 vs. NCBI nr
Match: XP_008455726.1 (PREDICTED: probable auxin efflux carrier component 1b [Cucumis melo])

HSP 1 Score: 1139.8 bits (2947), Expect = 0.0e+00
Identity = 596/596 (100.00%), Postives = 596/596 (100.00%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS
Sbjct: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  SNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120
           SNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL
Sbjct: 61  SNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD
Sbjct: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180

Query: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA 240
           ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA
Sbjct: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA 240

Query: 241 EIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGLKSRGISGNVNGI 300
           EIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGLKSRGISGNVNGI
Sbjct: 241 EIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGLKSRGISGNVNGI 300

Query: 301 FPSSNGYPAPASGGLFSPVTGPAATKKRNGGDGGKDLHMFVWSSSASPVSEGGINVFRSG 360
           FPSSNGYPAPASGGLFSPVTGPAATKKRNGGDGGKDLHMFVWSSSASPVSEGGINVFRSG
Sbjct: 301 FPSSNGYPAPASGGLFSPVTGPAATKKRNGGDGGKDLHMFVWSSSASPVSEGGINVFRSG 360

Query: 361 DYDGPGAGGMNQKDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKNGDD 420
           DYDGPGAGGMNQKDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKNGDD
Sbjct: 361 DYDGPGAGGMNQKDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKNGDD 420

Query: 421 TAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPAIVARS 480
           TAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPAIVARS
Sbjct: 421 TAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPAIVARS 480

Query: 481 IAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIAVGLRG 540
           IAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIAVGLRG
Sbjct: 481 IAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIAVGLRG 540

Query: 541 VLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL 597
           VLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL
Sbjct: 541 VLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL 596

BLAST of Cmc08g0222891 vs. NCBI nr
Match: XP_004144328.1 (probable auxin efflux carrier component 1b [Cucumis sativus] >KGN54700.1 hypothetical protein Csa_012183 [Cucumis sativus])

HSP 1 Score: 1128.2 bits (2917), Expect = 0.0e+00
Identity = 589/596 (98.83%), Postives = 592/596 (99.33%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS
Sbjct: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  SNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120
           SNNPFSMNLRFIAADTLQKLIVL ALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL
Sbjct: 61  SNNPFSMNLRFIAADTLQKLIVLVALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD
Sbjct: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180

Query: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA 240
           ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA
Sbjct: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA 240

Query: 241 EIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGLKSRGISGNVNGI 300
           EIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGG+K RGISGNVNGI
Sbjct: 241 EIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGMKGRGISGNVNGI 300

Query: 301 FPSSNGYPAPASGGLFSPVTGPAATKKRNGGDGGKDLHMFVWSSSASPVSEGGINVFRSG 360
           FPS+NGYPAPASGGLFSPVTGPAATKKRNGGDGGKDLHMFVWSSSASPVSEGGINVFRSG
Sbjct: 301 FPSANGYPAPASGGLFSPVTGPAATKKRNGGDGGKDLHMFVWSSSASPVSEGGINVFRSG 360

Query: 361 DYDGPGAGGMNQKDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKNGDD 420
           DYD PGAGGMN KDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPK+GDD
Sbjct: 361 DYDAPGAGGMNPKDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKHGDD 420

Query: 421 TAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPAIVARS 480
           TAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPAIVARS
Sbjct: 421 TAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPAIVARS 480

Query: 481 IAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIAVGLRG 540
           IAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIAVGLRG
Sbjct: 481 IAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIAVGLRG 540

Query: 541 VLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL 597
           VLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL
Sbjct: 541 VLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL 596

BLAST of Cmc08g0222891 vs. NCBI nr
Match: BAJ10464.1 (auxin efflux facilitator [Cucumis sativus])

HSP 1 Score: 1126.7 bits (2913), Expect = 0.0e+00
Identity = 588/596 (98.66%), Postives = 591/596 (99.16%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS
Sbjct: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  SNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120
           SNNPFSMNLRFIAADTLQKLIVL ALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL
Sbjct: 61  SNNPFSMNLRFIAADTLQKLIVLVALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD
Sbjct: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180

Query: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA 240
           ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA
Sbjct: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA 240

Query: 241 EIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGLKSRGISGNVNGI 300
           EIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGG+K RGISGNVNGI
Sbjct: 241 EIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGMKGRGISGNVNGI 300

Query: 301 FPSSNGYPAPASGGLFSPVTGPAATKKRNGGDGGKDLHMFVWSSSASPVSEGGINVFRSG 360
           FPS+NGYPAPASGGLFSP TGPAATKKRNGGDGGKDLHMFVWSSSASPVSEGGINVFRSG
Sbjct: 301 FPSANGYPAPASGGLFSPATGPAATKKRNGGDGGKDLHMFVWSSSASPVSEGGINVFRSG 360

Query: 361 DYDGPGAGGMNQKDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKNGDD 420
           DYD PGAGGMN KDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPK+GDD
Sbjct: 361 DYDAPGAGGMNPKDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKHGDD 420

Query: 421 TAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPAIVARS 480
           TAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPAIVARS
Sbjct: 421 TAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPAIVARS 480

Query: 481 IAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIAVGLRG 540
           IAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIAVGLRG
Sbjct: 481 IAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIAVGLRG 540

Query: 541 VLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL 597
           VLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL
Sbjct: 541 VLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL 596

BLAST of Cmc08g0222891 vs. NCBI nr
Match: KAA0025909.1 (putative auxin efflux carrier component 1b [Cucumis melo var. makuwa] >TYK27803.1 putative auxin efflux carrier component 1b [Cucumis melo var. makuwa])

HSP 1 Score: 1106.7 bits (2861), Expect = 0.0e+00
Identity = 575/575 (100.00%), Postives = 575/575 (100.00%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS
Sbjct: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  SNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120
           SNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL
Sbjct: 61  SNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD
Sbjct: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180

Query: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA 240
           ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA
Sbjct: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA 240

Query: 241 EIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGLKSRGISGNVNGI 300
           EIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGLKSRGISGNVNGI
Sbjct: 241 EIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGLKSRGISGNVNGI 300

Query: 301 FPSSNGYPAPASGGLFSPVTGPAATKKRNGGDGGKDLHMFVWSSSASPVSEGGINVFRSG 360
           FPSSNGYPAPASGGLFSPVTGPAATKKRNGGDGGKDLHMFVWSSSASPVSEGGINVFRSG
Sbjct: 301 FPSSNGYPAPASGGLFSPVTGPAATKKRNGGDGGKDLHMFVWSSSASPVSEGGINVFRSG 360

Query: 361 DYDGPGAGGMNQKDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKNGDD 420
           DYDGPGAGGMNQKDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKNGDD
Sbjct: 361 DYDGPGAGGMNQKDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKNGDD 420

Query: 421 TAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPAIVARS 480
           TAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPAIVARS
Sbjct: 421 TAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPAIVARS 480

Query: 481 IAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIAVGLRG 540
           IAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIAVGLRG
Sbjct: 481 IAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIAVGLRG 540

Query: 541 VLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTG 576
           VLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTG
Sbjct: 541 VLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTG 575

BLAST of Cmc08g0222891 vs. NCBI nr
Match: XP_038880940.1 (probable auxin efflux carrier component 1b isoform X2 [Benincasa hispida])

HSP 1 Score: 1096.3 bits (2834), Expect = 0.0e+00
Identity = 579/601 (96.34%), Postives = 587/601 (97.67%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS
Sbjct: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  SNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120
           +NNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL
Sbjct: 61  TNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD
Sbjct: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180

Query: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA 240
           ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA
Sbjct: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA 240

Query: 241 EIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGLKSRGISG--NVN 300
           EIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGG+K RGISG  NVN
Sbjct: 241 EIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGMKGRGISGNVNVN 300

Query: 301 GIFPSSNGYPAPASGGLFSPVTGPAATKKR-NGGDGGKDLHMFVWSSSASPVSEGGINVF 360
           G+FPSS GYPAPAS GLFSPVTGPAA KKR NGGDGGKDLHMFVWSSSASPVSEGGINVF
Sbjct: 301 GVFPSSTGYPAPASAGLFSPVTGPAAAKKRVNGGDGGKDLHMFVWSSSASPVSEGGINVF 360

Query: 361 RSGDYD--GPGAGGMNQKDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHP 420
           RSGDYD  G G GGMN+KDF+++G DEFSFGNKSAVNGGGHDGGPVLSKLGSSST ELHP
Sbjct: 361 RSGDYDGGGGGGGGMNRKDFDDFGRDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTTELHP 420

Query: 421 KNGDDTAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPA 480
           KNGDDTAESKPT+MPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNI MPA
Sbjct: 421 KNGDDTAESKPTTMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIVMPA 480

Query: 481 IVARSIAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIA 540
           IVARSIAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIA
Sbjct: 481 IVARSIAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIA 540

Query: 541 VGLRGVLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLG 597
           VGLRGVLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLG
Sbjct: 541 VGLRGVLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLG 600

BLAST of Cmc08g0222891 vs. ExPASy Swiss-Prot
Match: Q0IQA5 (Probable auxin efflux carrier component 1d OS=Oryza sativa subsp. japonica OX=39947 GN=PIN1D PE=2 SV=2)

HSP 1 Score: 717.2 bits (1850), Expect = 1.5e-205
Identity = 419/609 (68.80%), Postives = 465/609 (76.35%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MI+V DLYHVLTAVVPLYVAM LAY SV+WW+IFSPDQCSGINRFVALFAVPLLSFHFIS
Sbjct: 1   MITVVDLYHVLTAVVPLYVAMTLAYASVRWWRIFSPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  SNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120
           +NNPF+MNLRF+AADTLQKLIVLA LA+W  LS++GSL+W ITLFSLSTLPNTLVMGIPL
Sbjct: 61  TNNPFAMNLRFLAADTLQKLIVLALLALWCRLSARGSLDWLITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMY-------GDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIV 180
           LKGMY       G  +G+LMVQIVVLQCIIWYTLMLFLFEYRGARLL+ EQFPDTA  IV
Sbjct: 121 LKGMYAAAGAAAGADSGSLMVQIVVLQCIIWYTLMLFLFEYRGARLLVMEQFPDTAASIV 180

Query: 181 SFRVDSDILSL----DGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHG-GGGGV 240
           SFRVDSD++SL     G   LQ EAE+G+DGK+RV VRKSTSSRSE  +  SHG      
Sbjct: 181 SFRVDSDVVSLAGGGGGAAELQAEAEVGDDGKMRVTVRKSTSSRSE--AACSHGTQSHSQ 240

Query: 241 SLTPRPSNLTNAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGL 300
           S+ PR SNL+  EIYSLQSSRNPTPRGSSFNH++FF        +  GN  + DEE G  
Sbjct: 241 SMQPRVSNLSGVEIYSLQSSRNPTPRGSSFNHAEFF--------NIVGNGKHGDEEKGA- 300

Query: 301 KSRGISGNVNGIFPSSNGYPAPASGGLFSPVTGPAATKKRNGGDGGKDLHMFVWSSSASP 360
                                 A GG  SP   P   K+       KDLHMFVWSSSASP
Sbjct: 301 ----------------------AGGGGHSP--QPVVGKR-------KDLHMFVWSSSASP 360

Query: 361 VSEGGINVFRSGDYDGPGAGGMNQKDF-NEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGS 420
           VSE       +G     G GG +  D       DE+SFGNK+         GP LSKLGS
Sbjct: 361 VSERAAAAAAAGAVHVFGGGGADHGDAKGAQAYDEYSFGNKN------EKDGPTLSKLGS 420

Query: 421 SSTAELHPKNGDDTAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISF 480
           +STA+L PK   D  E +  +MPPASVMTRLILIMVWRKLIRNPNTYSSL+G+ WSL+S+
Sbjct: 421 NSTAQLRPK---DDGEGRAAAMPPASVMTRLILIMVWRKLIRNPNTYSSLLGVIWSLVSY 480

Query: 481 RWNIAMPAIVARSIAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPA 540
           RW I MPAI+ARSI+ILSDAGLGMAMFSLGLFMALQP+IIACGN++AS+AMAVRF+ GPA
Sbjct: 481 RWGIEMPAIIARSISILSDAGLGMAMFSLGLFMALQPRIIACGNSLASYAMAVRFLVGPA 540

Query: 541 VMAAASIAVGLRGVLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPIT 597
           VMAAASIAVGLRGVLLHIAIVQAALPQGIVPFVFAKEYNVHP+ILST VIFGMLIALPIT
Sbjct: 541 VMAAASIAVGLRGVLLHIAIVQAALPQGIVPFVFAKEYNVHPNILSTAVIFGMLIALPIT 558

BLAST of Cmc08g0222891 vs. ExPASy Swiss-Prot
Match: Q67UL3 (Probable auxin efflux carrier component 1c OS=Oryza sativa subsp. japonica OX=39947 GN=PIN1C PE=2 SV=1)

HSP 1 Score: 715.7 bits (1846), Expect = 4.4e-205
Identity = 410/609 (67.32%), Postives = 470/609 (77.18%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MI+ +D YHV+TA+VPLYVAMILAYGSVKWW+IF+PDQCSGINRFVALFAVPLLSFHFIS
Sbjct: 1   MITGADFYHVMTAMVPLYVAMILAYGSVKWWRIFTPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  SNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120
           +NNP++MNLRFIAADTLQKLIVLA L +WSHLS +GSLEW+ITLFSLSTLPNTLVMGIPL
Sbjct: 61  TNNPYTMNLRFIAADTLQKLIVLALLTLWSHLSRRGSLEWTITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           LKGMYG+ +G+LMVQIVVLQCIIWYTLMLF+FEYRGAR+LI EQFPDTAG I S  VD+D
Sbjct: 121 LKGMYGEFSGSLMVQIVVLQCIIWYTLMLFMFEYRGARILITEQFPDTAGAIASIVVDAD 180

Query: 181 ILSLDG-KEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTN 240
           ++SLDG ++ ++TEAE+ EDGK+ V VR+S +SRS+V+SRRS G     S TPRPSNLTN
Sbjct: 181 VVSLDGRRDMIETEAEVKEDGKIHVTVRRSNASRSDVYSRRSMGFS---STTPRPSNLTN 240

Query: 241 AEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGLKSRGISGNVNG 300
           AEIYSLQSSRNPTPRGSSFNH+DF+  +   R SNF     F    G    R  +   + 
Sbjct: 241 AEIYSLQSSRNPTPRGSSFNHTDFY--SMVGRSSNFAAGDAFGVRTGA-TPRPSNYEEDA 300

Query: 301 IFPSSNG-----YPAPASGGLFSPVTGPAATKKRNGGDGGKDLHMFVWSSSASPVSEGGI 360
             P+  G     YPAP       P    AA  +  G D GKDLHMFVWSSSASPVS+   
Sbjct: 301 AAPNKAGSKYGQYPAPNPAMAAPPKPKKAANGQAKGED-GKDLHMFVWSSSASPVSD--- 360

Query: 361 NVFRSG-DYDGPGA------GGMNQKDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGS 420
            VF +G +Y+   A         + +  +    D+FSFGN+         G         
Sbjct: 361 -VFGNGAEYNDAAAVKEVRMAVASPRKADGVERDDFSFGNRGVAERDAEAGDE------K 420

Query: 421 SSTAELHPKNGDDTAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISF 480
           S  A +  ++G       PT+MPP SVMTRLILIMVWRKLIRNPNTYSSLIGL WSL+ F
Sbjct: 421 SVAAAVSGEHGKPGLTPAPTAMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGLIWSLVCF 480

Query: 481 RWNIAMPAIVARSIAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPA 540
           RWN  MPAI+ +SI+ILSDAGLGMAMFSLGLFMALQP+IIACGN +A+FAMAVRF+TGPA
Sbjct: 481 RWNFEMPAIILKSISILSDAGLGMAMFSLGLFMALQPRIIACGNKVATFAMAVRFLTGPA 540

Query: 541 VMAAASIAVGLRGVLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPIT 597
           VMAAASIAVGLRG LLH+AIVQAALPQGIVPFVFAKEY+VHPDILST VIFGMLIALPIT
Sbjct: 541 VMAAASIAVGLRGTLLHVAIVQAALPQGIVPFVFAKEYSVHPDILSTAVIFGMLIALPIT 592

BLAST of Cmc08g0222891 vs. ExPASy Swiss-Prot
Match: Q9C6B8 (Auxin efflux carrier component 1 OS=Arabidopsis thaliana OX=3702 GN=PIN1 PE=1 SV=1)

HSP 1 Score: 714.5 bits (1843), Expect = 9.8e-205
Identity = 417/646 (64.55%), Postives = 486/646 (75.23%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MI+ +D YHV+TA+VPLYVAMILAYGSVKWWKIF+PDQCSGINRFVALFAVPLLSFHFI+
Sbjct: 1   MITAADFYHVMTAMVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFIA 60

Query: 61  SNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120
           +NNP++MNLRF+AAD+LQK+IVL+ L +W  LS  GSL+W+ITLFSLSTLPNTLVMGIPL
Sbjct: 61  ANNPYAMNLRFLAADSLQKVIVLSLLFLWCKLSRNGSLDWTITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           LKGMYG+ +G LMVQIVVLQCIIWYTLMLFLFEYRGA+LLI+EQFPDTAG IVS  VDSD
Sbjct: 121 LKGMYGNFSGDLMVQIVVLQCIIWYTLMLFLFEYRGAKLLISEQFPDTAGSIVSIHVDSD 180

Query: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA 240
           I+SLDG++PL+TEAEI EDGKL V VR+S +SRS+++SRRS     G+S TPRPSNLTNA
Sbjct: 181 IMSLDGRQPLETEAEIKEDGKLHVTVRRSNASRSDIYSRRSQ----GLSATPRPSNLTNA 240

Query: 241 EIYSLQSSRNPTPRGSSFNHSDFF--LGNASPRHSNFGN----FG---------NFDEEN 300
           EIYSLQSSRNPTPRGSSFNH+DF+  + +   R+SNFG     FG         N++E+ 
Sbjct: 241 EIYSLQSSRNPTPRGSSFNHTDFYSMMASGGGRNSNFGPGEAVFGSKGPTPRPSNYEEDG 300

Query: 301 GGLK--SRGISGNVNGIFPSSNG--------YPAPASGGLFSPVTG-----------PAA 360
           G  K  + G +         S G        YPAP + G+FSP TG           P  
Sbjct: 301 GPAKPTAAGTAAGAGRFHYQSGGSGGGGGAHYPAP-NPGMFSPNTGGGGGTAAKGNAPVV 360

Query: 361 TKKRNGGDGGKDLHMFVWSSSASPVSE--GGINVFRSGDYDGPGAGGMNQKDF------- 420
             KR  G+ G+DLHMFVWSSSASPVS+  GG       DY    A   +QKD        
Sbjct: 361 GGKRQDGN-GRDLHMFVWSSSASPVSDVFGGGGGNHHADYS--TATNDHQKDVKISVPQG 420

Query: 421 ----NEY-GGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKNGDDTAESKPTSMP 480
               N+Y   +EFSFGNK        D   VL+  G ++ +       + T ++K   MP
Sbjct: 421 NSNDNQYVEREEFSFGNKD-------DDSKVLATDGGNNIS-------NKTTQAK--VMP 480

Query: 481 PASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPAIVARSIAILSDAGLG 540
           P SVMTRLILIMVWRKLIRNPN+YSSL G+ WSLISF+WNI MPA++A+SI+ILSDAGLG
Sbjct: 481 PTSVMTRLILIMVWRKLIRNPNSYSSLFGITWSLISFKWNIEMPALIAKSISILSDAGLG 540

Query: 541 MAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIAVGLRGVLLHIAIVQA 597
           MAMFSLGLFMAL P+IIACGN  A+FA A+RF+ GPAVM  AS AVGLRGVLLH+AI+QA
Sbjct: 541 MAMFSLGLFMALNPRIIACGNRRAAFAAAMRFVVGPAVMLVASYAVGLRGVLLHVAIIQA 600

BLAST of Cmc08g0222891 vs. ExPASy Swiss-Prot
Match: P0C0X5 (Probable auxin efflux carrier component 1b OS=Oryza sativa subsp. japonica OX=39947 GN=PIN1B PE=2 SV=1)

HSP 1 Score: 713.4 bits (1840), Expect = 2.2e-204
Identity = 419/612 (68.46%), Postives = 465/612 (75.98%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MI+V DLYHVLTAVVPLYVAM LAY SV+WW+IFSPDQCSGINRFVALFAVPLLSFHFIS
Sbjct: 1   MITVVDLYHVLTAVVPLYVAMTLAYASVRWWRIFSPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  SNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120
           +NNPF+MNLRF+AADTLQKLIVLA LA+W  LS++GSL+W ITLFSLSTLPNTLVMGIPL
Sbjct: 61  TNNPFAMNLRFLAADTLQKLIVLALLALWCRLSARGSLDWLITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDS----TGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFR 180
           LKGMY  +    +G+LMVQIVVLQCIIWYTLMLFLFEYRGARLL+ EQFPDTA  IVSFR
Sbjct: 121 LKGMYAAAADVDSGSLMVQIVVLQCIIWYTLMLFLFEYRGARLLVMEQFPDTAASIVSFR 180

Query: 181 VDSDILSL----DGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHG-GGGGVSLT 240
           VDSD++SL     G   LQ EAE+G+DG++RV VRKSTSSRSE  +  SHG      S+ 
Sbjct: 181 VDSDVVSLAGGGGGAAELQAEAEVGDDGRMRVTVRKSTSSRSE--AACSHGTQSHSQSMQ 240

Query: 241 PRPSNLTNAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGLKSR 300
           PR SNL+  EIYSLQSSRNPTPRGSSFNH++FF        +  GN    DEE G     
Sbjct: 241 PRVSNLSGVEIYSLQSSRNPTPRGSSFNHAEFF--------NIVGNGKQGDEEKGA---- 300

Query: 301 GISGNVNGIFPSSNGYPAPASGGLFSPVTGPAATKKRNGGDGGKDLHMFVWSSSASPVSE 360
                              A GG  SP   P   K+       KDLHMFVWSSSASPVSE
Sbjct: 301 -------------------AGGGGHSP--QPVVGKR-------KDLHMFVWSSSASPVSE 360

Query: 361 -------GGINVFRSGDYDGPGAGGMNQKDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSK 420
                  G ++VF  G  D   A G           DE+SFGNK+         GP LSK
Sbjct: 361 RAAAAAAGAVHVFGGGGADHGDAKGAQ-------AYDEYSFGNKN------EKDGPTLSK 420

Query: 421 LGSSSTAELHPKNGDDTAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSL 480
           LGS+STA+L PK   D  E    +MPPASVMTRLILIMVWRKLIRNPNTYSSL+G+ WSL
Sbjct: 421 LGSNSTAQLRPK---DDGEGMAAAMPPASVMTRLILIMVWRKLIRNPNTYSSLLGVIWSL 480

Query: 481 ISFRWNIAMPAIVARSIAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFIT 540
           +S+RW I MPAI+ARSI+ILSDAGLGMAMFSLGLFMALQP+IIACGN++AS+AMAVRF+ 
Sbjct: 481 VSYRWGIEMPAIIARSISILSDAGLGMAMFSLGLFMALQPRIIACGNSLASYAMAVRFLV 540

Query: 541 GPAVMAAASIAVGLRGVLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIAL 597
           GPAVMAAASIAVGLRGVLLHIAIVQAALPQGIVPFVFAKEYNVHP+ILST VIFGMLIAL
Sbjct: 541 GPAVMAAASIAVGLRGVLLHIAIVQAALPQGIVPFVFAKEYNVHPNILSTAVIFGMLIAL 554

BLAST of Cmc08g0222891 vs. ExPASy Swiss-Prot
Match: Q5SMQ9 (Auxin efflux carrier component 1a OS=Oryza sativa subsp. japonica OX=39947 GN=PIN1A PE=2 SV=1)

HSP 1 Score: 704.5 bits (1817), Expect = 1.0e-201
Identity = 404/615 (65.69%), Postives = 466/615 (75.77%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MI+ +D YHV+TA+VPLYVAMILAYGSVKWW+IF+PDQCSGINRFVALFAVPLLSFHFIS
Sbjct: 1   MITAADFYHVMTAMVPLYVAMILAYGSVKWWRIFTPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  SNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120
           +NNP++MNLRFIAADTLQKL+VLA L  WSHLS +GSLEW+ITLFSLSTLPNTLVMGIPL
Sbjct: 61  TNNPYTMNLRFIAADTLQKLMVLAMLTAWSHLSRRGSLEWTITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           LKGMYG+ +G+LMVQIVVLQCIIWYTLMLF+FEYRGAR+LI EQFPDTA  I S  VD D
Sbjct: 121 LKGMYGEFSGSLMVQIVVLQCIIWYTLMLFMFEYRGARMLITEQFPDTAANIASIVVDPD 180

Query: 181 ILSLDG-KEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTN 240
           ++SLDG ++ ++TE E+ EDG++ V VR+S +SRS+++SRRS G     S TPRPSNLTN
Sbjct: 181 VVSLDGRRDAIETETEVKEDGRIHVTVRRSNASRSDIYSRRSMGFS---STTPRPSNLTN 240

Query: 241 AEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGL----KSRGISG 300
           AEIYSLQSSRNPTPRGSSFNH+DF+  +   R SNFG    F    G           + 
Sbjct: 241 AEIYSLQSSRNPTPRGSSFNHTDFY--SMVGRSSNFGAADAFGVRTGATPRPSNYEDDAS 300

Query: 301 NVNGIFPSSNGYPA----PASGGLFSPVTGPAATKKRNGGDGGKDLHMFVWSSSASPVSE 360
                 P+SN  P     PA     S     A     NG   G+DLHMFVWSSSASPVS+
Sbjct: 301 KPKYPLPASNAAPMAGHYPAPNPAVSSAPKGAKKAATNGQAKGEDLHMFVWSSSASPVSD 360

Query: 361 GGINVFRSG--DYDGPGA--------GGMNQKDFNEYGGDEFSFGNKSAVNGGGHDGGPV 420
               VF  G  DY+   A        G  +++D+ E   D+FSFGN+  ++     G   
Sbjct: 361 ----VFGGGAPDYNDAAAVKSPRKMDGAKDREDYVE--RDDFSFGNRGVMDRDAEAGD-- 420

Query: 421 LSKLGSSSTAELHPKNGDDTAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLA 480
             K  +++ A+         A + PT+MPP SVMTRLILIMVWRKLIRNPNTYSSLIGL 
Sbjct: 421 -EKAAAAAGAD------PSKAMAAPTAMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGLI 480

Query: 481 WSLISFRWNIAMPAIVARSIAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVR 540
           WSL+ FRWN  MPAIV +SI+ILSDAGLGMAMFSLGLFMALQP IIACGN +A++AMAVR
Sbjct: 481 WSLVCFRWNFEMPAIVLKSISILSDAGLGMAMFSLGLFMALQPHIIACGNKVATYAMAVR 540

Query: 541 FITGPAVMAAASIAVGLRGVLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGML 597
           F+ GPAVMAAAS AVGLRG LLH+AIVQAALPQGIVPFVFAKEY+VHP ILST VIFGML
Sbjct: 541 FLAGPAVMAAASFAVGLRGTLLHVAIVQAALPQGIVPFVFAKEYSVHPSILSTAVIFGML 595

BLAST of Cmc08g0222891 vs. ExPASy TrEMBL
Match: A0A1S3C1I4 (Auxin efflux carrier component OS=Cucumis melo OX=3656 GN=LOC103495829 PE=3 SV=1)

HSP 1 Score: 1139.8 bits (2947), Expect = 0.0e+00
Identity = 596/596 (100.00%), Postives = 596/596 (100.00%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS
Sbjct: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  SNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120
           SNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL
Sbjct: 61  SNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD
Sbjct: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180

Query: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA 240
           ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA
Sbjct: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA 240

Query: 241 EIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGLKSRGISGNVNGI 300
           EIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGLKSRGISGNVNGI
Sbjct: 241 EIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGLKSRGISGNVNGI 300

Query: 301 FPSSNGYPAPASGGLFSPVTGPAATKKRNGGDGGKDLHMFVWSSSASPVSEGGINVFRSG 360
           FPSSNGYPAPASGGLFSPVTGPAATKKRNGGDGGKDLHMFVWSSSASPVSEGGINVFRSG
Sbjct: 301 FPSSNGYPAPASGGLFSPVTGPAATKKRNGGDGGKDLHMFVWSSSASPVSEGGINVFRSG 360

Query: 361 DYDGPGAGGMNQKDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKNGDD 420
           DYDGPGAGGMNQKDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKNGDD
Sbjct: 361 DYDGPGAGGMNQKDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKNGDD 420

Query: 421 TAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPAIVARS 480
           TAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPAIVARS
Sbjct: 421 TAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPAIVARS 480

Query: 481 IAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIAVGLRG 540
           IAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIAVGLRG
Sbjct: 481 IAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIAVGLRG 540

Query: 541 VLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL 597
           VLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL
Sbjct: 541 VLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL 596

BLAST of Cmc08g0222891 vs. ExPASy TrEMBL
Match: A0A0A0KYF4 (Auxin efflux carrier component OS=Cucumis sativus OX=3659 GN=Csa_4G430820 PE=3 SV=1)

HSP 1 Score: 1128.2 bits (2917), Expect = 0.0e+00
Identity = 589/596 (98.83%), Postives = 592/596 (99.33%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS
Sbjct: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  SNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120
           SNNPFSMNLRFIAADTLQKLIVL ALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL
Sbjct: 61  SNNPFSMNLRFIAADTLQKLIVLVALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD
Sbjct: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180

Query: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA 240
           ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA
Sbjct: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA 240

Query: 241 EIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGLKSRGISGNVNGI 300
           EIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGG+K RGISGNVNGI
Sbjct: 241 EIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGMKGRGISGNVNGI 300

Query: 301 FPSSNGYPAPASGGLFSPVTGPAATKKRNGGDGGKDLHMFVWSSSASPVSEGGINVFRSG 360
           FPS+NGYPAPASGGLFSPVTGPAATKKRNGGDGGKDLHMFVWSSSASPVSEGGINVFRSG
Sbjct: 301 FPSANGYPAPASGGLFSPVTGPAATKKRNGGDGGKDLHMFVWSSSASPVSEGGINVFRSG 360

Query: 361 DYDGPGAGGMNQKDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKNGDD 420
           DYD PGAGGMN KDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPK+GDD
Sbjct: 361 DYDAPGAGGMNPKDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKHGDD 420

Query: 421 TAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPAIVARS 480
           TAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPAIVARS
Sbjct: 421 TAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPAIVARS 480

Query: 481 IAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIAVGLRG 540
           IAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIAVGLRG
Sbjct: 481 IAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIAVGLRG 540

Query: 541 VLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL 597
           VLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL
Sbjct: 541 VLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL 596

BLAST of Cmc08g0222891 vs. ExPASy TrEMBL
Match: D7URM4 (Auxin efflux carrier component OS=Cucumis sativus OX=3659 GN=CsPIN4 PE=2 SV=1)

HSP 1 Score: 1126.7 bits (2913), Expect = 0.0e+00
Identity = 588/596 (98.66%), Postives = 591/596 (99.16%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS
Sbjct: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  SNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120
           SNNPFSMNLRFIAADTLQKLIVL ALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL
Sbjct: 61  SNNPFSMNLRFIAADTLQKLIVLVALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD
Sbjct: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180

Query: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA 240
           ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA
Sbjct: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA 240

Query: 241 EIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGLKSRGISGNVNGI 300
           EIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGG+K RGISGNVNGI
Sbjct: 241 EIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGMKGRGISGNVNGI 300

Query: 301 FPSSNGYPAPASGGLFSPVTGPAATKKRNGGDGGKDLHMFVWSSSASPVSEGGINVFRSG 360
           FPS+NGYPAPASGGLFSP TGPAATKKRNGGDGGKDLHMFVWSSSASPVSEGGINVFRSG
Sbjct: 301 FPSANGYPAPASGGLFSPATGPAATKKRNGGDGGKDLHMFVWSSSASPVSEGGINVFRSG 360

Query: 361 DYDGPGAGGMNQKDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKNGDD 420
           DYD PGAGGMN KDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPK+GDD
Sbjct: 361 DYDAPGAGGMNPKDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKHGDD 420

Query: 421 TAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPAIVARS 480
           TAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPAIVARS
Sbjct: 421 TAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPAIVARS 480

Query: 481 IAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIAVGLRG 540
           IAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIAVGLRG
Sbjct: 481 IAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIAVGLRG 540

Query: 541 VLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL 597
           VLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL
Sbjct: 541 VLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL 596

BLAST of Cmc08g0222891 vs. ExPASy TrEMBL
Match: A0A5A7SKX8 (Auxin efflux carrier component OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold749G00260 PE=3 SV=1)

HSP 1 Score: 1106.7 bits (2861), Expect = 0.0e+00
Identity = 575/575 (100.00%), Postives = 575/575 (100.00%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS
Sbjct: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  SNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120
           SNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL
Sbjct: 61  SNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD
Sbjct: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180

Query: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA 240
           ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA
Sbjct: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA 240

Query: 241 EIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGLKSRGISGNVNGI 300
           EIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGLKSRGISGNVNGI
Sbjct: 241 EIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGLKSRGISGNVNGI 300

Query: 301 FPSSNGYPAPASGGLFSPVTGPAATKKRNGGDGGKDLHMFVWSSSASPVSEGGINVFRSG 360
           FPSSNGYPAPASGGLFSPVTGPAATKKRNGGDGGKDLHMFVWSSSASPVSEGGINVFRSG
Sbjct: 301 FPSSNGYPAPASGGLFSPVTGPAATKKRNGGDGGKDLHMFVWSSSASPVSEGGINVFRSG 360

Query: 361 DYDGPGAGGMNQKDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKNGDD 420
           DYDGPGAGGMNQKDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKNGDD
Sbjct: 361 DYDGPGAGGMNQKDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKNGDD 420

Query: 421 TAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPAIVARS 480
           TAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPAIVARS
Sbjct: 421 TAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPAIVARS 480

Query: 481 IAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIAVGLRG 540
           IAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIAVGLRG
Sbjct: 481 IAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIAVGLRG 540

Query: 541 VLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTG 576
           VLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTG
Sbjct: 541 VLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTG 575

BLAST of Cmc08g0222891 vs. ExPASy TrEMBL
Match: A0A6J1HQV8 (Auxin efflux carrier component OS=Cucurbita maxima OX=3661 GN=LOC111465879 PE=3 SV=1)

HSP 1 Score: 1066.2 bits (2756), Expect = 4.9e-308
Identity = 563/600 (93.83%), Postives = 577/600 (96.17%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS
Sbjct: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  SNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120
           SNNPFSMN RFIAADTLQKLIVLAALAVWSHLSSKGSLEW+ITLFSLSTLPNTLVMGIPL
Sbjct: 61  SNNPFSMNFRFIAADTLQKLIVLAALAVWSHLSSKGSLEWAITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD
Sbjct: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180

Query: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSH--GGGGGVSLTPRPSNLT 240
           ILSLDGKEPLQTEAEIGEDGKLRVVVRKS SSRSEVFSRRSH  GGGGG SLTPRPSNLT
Sbjct: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSASSRSEVFSRRSHGGGGGGGASLTPRPSNLT 240

Query: 241 NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGLKSRGISGNVN 300
           NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSN+GNFGNFDEE+GG K R I+GNV 
Sbjct: 241 NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNYGNFGNFDEESGGFKGRAINGNV- 300

Query: 301 GIFPSSNGYPAPASGGLFSPVTGPAATKKR--NGGDGGKDLHMFVWSSSASPVSEGGINV 360
                 NGYPAPASGGLFSP+TGPAA  K+  +GGDGGKDLHMFVWSSSASPVSEGG+NV
Sbjct: 301 ------NGYPAPASGGLFSPMTGPAAAAKKRVHGGDGGKDLHMFVWSSSASPVSEGGLNV 360

Query: 361 FRSGDYDGPGAGGMNQKDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPK 420
           FR GDYDG GAGGMNQKDF+E+G DEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPK
Sbjct: 361 FRGGDYDGAGAGGMNQKDFDEFGRDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPK 420

Query: 421 NGDDTAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPAI 480
           +GDDTAESKPT+MPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNI MPAI
Sbjct: 421 SGDDTAESKPTAMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIVMPAI 480

Query: 481 VARSIAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIAV 540
           VARSIAILSDAGLGMAMFSLGLFMALQPKIIACGNTIA+FAMAVRFITGPAVMAAASIAV
Sbjct: 481 VARSIAILSDAGLGMAMFSLGLFMALQPKIIACGNTIATFAMAVRFITGPAVMAAASIAV 540

Query: 541 GLRGVLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL 597
           GL+GVLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL
Sbjct: 541 GLKGVLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL 593

BLAST of Cmc08g0222891 vs. TAIR 10
Match: AT1G73590.1 (Auxin efflux carrier family protein )

HSP 1 Score: 714.5 bits (1843), Expect = 6.9e-206
Identity = 417/646 (64.55%), Postives = 486/646 (75.23%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MI+ +D YHV+TA+VPLYVAMILAYGSVKWWKIF+PDQCSGINRFVALFAVPLLSFHFI+
Sbjct: 1   MITAADFYHVMTAMVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFIA 60

Query: 61  SNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120
           +NNP++MNLRF+AAD+LQK+IVL+ L +W  LS  GSL+W+ITLFSLSTLPNTLVMGIPL
Sbjct: 61  ANNPYAMNLRFLAADSLQKVIVLSLLFLWCKLSRNGSLDWTITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           LKGMYG+ +G LMVQIVVLQCIIWYTLMLFLFEYRGA+LLI+EQFPDTAG IVS  VDSD
Sbjct: 121 LKGMYGNFSGDLMVQIVVLQCIIWYTLMLFLFEYRGAKLLISEQFPDTAGSIVSIHVDSD 180

Query: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA 240
           I+SLDG++PL+TEAEI EDGKL V VR+S +SRS+++SRRS     G+S TPRPSNLTNA
Sbjct: 181 IMSLDGRQPLETEAEIKEDGKLHVTVRRSNASRSDIYSRRSQ----GLSATPRPSNLTNA 240

Query: 241 EIYSLQSSRNPTPRGSSFNHSDFF--LGNASPRHSNFGN----FG---------NFDEEN 300
           EIYSLQSSRNPTPRGSSFNH+DF+  + +   R+SNFG     FG         N++E+ 
Sbjct: 241 EIYSLQSSRNPTPRGSSFNHTDFYSMMASGGGRNSNFGPGEAVFGSKGPTPRPSNYEEDG 300

Query: 301 GGLK--SRGISGNVNGIFPSSNG--------YPAPASGGLFSPVTG-----------PAA 360
           G  K  + G +         S G        YPAP + G+FSP TG           P  
Sbjct: 301 GPAKPTAAGTAAGAGRFHYQSGGSGGGGGAHYPAP-NPGMFSPNTGGGGGTAAKGNAPVV 360

Query: 361 TKKRNGGDGGKDLHMFVWSSSASPVSE--GGINVFRSGDYDGPGAGGMNQKDF------- 420
             KR  G+ G+DLHMFVWSSSASPVS+  GG       DY    A   +QKD        
Sbjct: 361 GGKRQDGN-GRDLHMFVWSSSASPVSDVFGGGGGNHHADYS--TATNDHQKDVKISVPQG 420

Query: 421 ----NEY-GGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKNGDDTAESKPTSMP 480
               N+Y   +EFSFGNK        D   VL+  G ++ +       + T ++K   MP
Sbjct: 421 NSNDNQYVEREEFSFGNKD-------DDSKVLATDGGNNIS-------NKTTQAK--VMP 480

Query: 481 PASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPAIVARSIAILSDAGLG 540
           P SVMTRLILIMVWRKLIRNPN+YSSL G+ WSLISF+WNI MPA++A+SI+ILSDAGLG
Sbjct: 481 PTSVMTRLILIMVWRKLIRNPNSYSSLFGITWSLISFKWNIEMPALIAKSISILSDAGLG 540

Query: 541 MAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIAVGLRGVLLHIAIVQA 597
           MAMFSLGLFMAL P+IIACGN  A+FA A+RF+ GPAVM  AS AVGLRGVLLH+AI+QA
Sbjct: 541 MAMFSLGLFMALNPRIIACGNRRAAFAAAMRFVVGPAVMLVASYAVGLRGVLLHVAIIQA 600

BLAST of Cmc08g0222891 vs. TAIR 10
Match: AT1G23080.3 (Auxin efflux carrier family protein )

HSP 1 Score: 681.0 bits (1756), Expect = 8.5e-196
Identity = 402/639 (62.91%), Postives = 468/639 (73.24%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MI+  DLY VLTAV+PLYVAMILAYGSV+WWKIFSPDQCSGINRFVA+FAVPLLSFHFIS
Sbjct: 1   MITWHDLYTVLTAVIPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  SNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120
           SNNP++MNLRFIAADTLQKLI+L  L +W++ +  GSLEWSIT+FSLSTLPNTLVMGIPL
Sbjct: 61  SNNPYAMNLRFIAADTLQKLIMLTLLIIWANFTRSGSLEWSITIFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           L  MYG+ +G+LMVQIVVLQCIIWYTL+LFLFEYRGA++LI EQFP+T   IVSF+V+SD
Sbjct: 121 LIAMYGEYSGSLMVQIVVLQCIIWYTLLLFLFEYRGAKILIMEQFPETGASIVSFKVESD 180

Query: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA 240
           ++SLDG + L+T+A+IG+DGKL V VRKS +      SRRS  GGGG ++TPRPSNLT A
Sbjct: 181 VVSLDGHDFLETDAQIGDDGKLHVTVRKSNA------SRRSFYGGGGTNMTPRPSNLTGA 240

Query: 241 EIYSLQSSRNPTPRGSSFNHSDFF--LGNASPRHSNFGNFGNFD--------------EE 300
           EIYSL    N TPRGS+FNHSDF+  +G    R SNFG    +               EE
Sbjct: 241 EIYSL----NTTPRGSNFNHSDFYSMMGFPGGRLSNFGPADMYSVQSSRGPTPRPSNFEE 300

Query: 301 NGGLKSRGISGNVNGIFPSSNGYPAPASGGLFSPVTGPAATKK------RNGGDGGKDLH 360
           +  + S    G   G  P S  YPAP         TG  A K+      ++  +  K+LH
Sbjct: 301 SCAMASSPRFGYYPGGAPGS--YPAPNPEFSTGNKTGSKAPKENHHHVGKSNSNDAKELH 360

Query: 361 MFVWSSSASPVSE-GGINVFRS-----GDYDGPG--------------AGGMNQKDFNEY 420
           MFVW S+ SPVS+  G+ V        G  D  G              AG MN     +Y
Sbjct: 361 MFVWGSNGSPVSDRAGLQVDNGANEQVGKSDQGGAKEIRMLISDHTQNAGPMN----GDY 420

Query: 421 GGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKNGDDTAESKPTS-MPPASVMTR 480
           GG+E S   K   NG        L KL  +STAEL+PK   +T E+ P   MPPASVMTR
Sbjct: 421 GGEEESERVKEVPNG--------LHKLRCNSTAELNPKEAIETGETVPVKHMPPASVMTR 480

Query: 481 LILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPAIVARSIAILSDAGLGMAMFSLG 540
           LILIMVWRKLIRNPNTYSSLIGL W+L++FRW++AMP I+ +SI+ILSDAGLGMAMFSLG
Sbjct: 481 LILIMVWRKLIRNPNTYSSLIGLIWALVAFRWDVAMPKIIQQSISILSDAGLGMAMFSLG 540

Query: 541 LFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIAVGLRGVLLHIAIVQAALPQGIV 597
           LFMALQPK+IACGN+ A+FAMAVRF TGPAVMA A++A+GLRG LL +AIVQAALPQGIV
Sbjct: 541 LFMALQPKLIACGNSTATFAMAVRFFTGPAVMAVAAMAIGLRGDLLRVAIVQAALPQGIV 600

BLAST of Cmc08g0222891 vs. TAIR 10
Match: AT1G23080.1 (Auxin efflux carrier family protein )

HSP 1 Score: 680.2 bits (1754), Expect = 1.4e-195
Identity = 402/643 (62.52%), Postives = 468/643 (72.78%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MI+  DLY VLTAV+PLYVAMILAYGSV+WWKIFSPDQCSGINRFVA+FAVPLLSFHFIS
Sbjct: 1   MITWHDLYTVLTAVIPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  SNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120
           SNNP++MNLRFIAADTLQKLI+L  L +W++ +  GSLEWSIT+FSLSTLPNTLVMGIPL
Sbjct: 61  SNNPYAMNLRFIAADTLQKLIMLTLLIIWANFTRSGSLEWSITIFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           L  MYG+ +G+LMVQIVVLQCIIWYTL+LFLFEYRGA++LI EQFP+T   IVSF+V+SD
Sbjct: 121 LIAMYGEYSGSLMVQIVVLQCIIWYTLLLFLFEYRGAKILIMEQFPETGASIVSFKVESD 180

Query: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA 240
           ++SLDG + L+T+A+IG+DGKL V VRKS +      SRRS  GGGG ++TPRPSNLT A
Sbjct: 181 VVSLDGHDFLETDAQIGDDGKLHVTVRKSNA------SRRSFYGGGGTNMTPRPSNLTGA 240

Query: 241 EIYSLQSSRNPTPRGSSFNHSDFF--LGNASPRHSNFGNFGNFD--------------EE 300
           EIYSL    N TPRGS+FNHSDF+  +G    R SNFG    +               EE
Sbjct: 241 EIYSL----NTTPRGSNFNHSDFYSMMGFPGGRLSNFGPADMYSVQSSRGPTPRPSNFEE 300

Query: 301 NGGLKSRGISGNVNGIFPSSNGYPAPASGGLFSPVTGPAATKK------RNGGDGGKDLH 360
           +  + S    G   G  P S  YPAP         TG  A K+      ++  +  K+LH
Sbjct: 301 SCAMASSPRFGYYPGGAPGS--YPAPNPEFSTGNKTGSKAPKENHHHVGKSNSNDAKELH 360

Query: 361 MFVWSSSASPVS-------------------EGGINVFRS--GDYDGPG---AGGMNQKD 420
           MFVW S+ SPVS                   +GG    R    D+   G   AG MN   
Sbjct: 361 MFVWGSNGSPVSDRAGLQVDNGANEQVGKSDQGGAKEIRMLISDHTQNGENKAGPMN--- 420

Query: 421 FNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKNGDDTAESKPTS-MPPAS 480
             +YGG+E S   K   NG        L KL  +STAEL+PK   +T E+ P   MPPAS
Sbjct: 421 -GDYGGEEESERVKEVPNG--------LHKLRCNSTAELNPKEAIETGETVPVKHMPPAS 480

Query: 481 VMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPAIVARSIAILSDAGLGMAM 540
           VMTRLILIMVWRKLIRNPNTYSSLIGL W+L++FRW++AMP I+ +SI+ILSDAGLGMAM
Sbjct: 481 VMTRLILIMVWRKLIRNPNTYSSLIGLIWALVAFRWDVAMPKIIQQSISILSDAGLGMAM 540

Query: 541 FSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIAVGLRGVLLHIAIVQAALP 597
           FSLGLFMALQPK+IACGN+ A+FAMAVRF TGPAVMA A++A+GLRG LL +AIVQAALP
Sbjct: 541 FSLGLFMALQPKLIACGNSTATFAMAVRFFTGPAVMAVAAMAIGLRGDLLRVAIVQAALP 600

BLAST of Cmc08g0222891 vs. TAIR 10
Match: AT2G01420.2 (Auxin efflux carrier family protein )

HSP 1 Score: 669.1 bits (1725), Expect = 3.3e-192
Identity = 398/647 (61.51%), Postives = 456/647 (70.48%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MI+  DLY VLTAVVPLYVAMILAYGSV+WWKIFSPDQCSGINRFVA+FAVPLLSFHFIS
Sbjct: 1   MITWHDLYTVLTAVVPLYVAMILAYGSVQWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  SNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120
           +N+P++MN RF+AADTLQK+I+L  LA+W++L+  GSLEW IT+FSLSTLPNTLVMGIPL
Sbjct: 61  TNDPYAMNFRFVAADTLQKIIMLVLLALWANLTKNGSLEWMITIFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           L  MYG   G+LMVQ+VVLQCIIWYTL+LFLFEYRGA+LLI EQFP+T   IVSF+V+SD
Sbjct: 121 LIAMYGTYAGSLMVQVVVLQCIIWYTLLLFLFEYRGAKLLIMEQFPETGASIVSFKVESD 180

Query: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA 240
           ++SLDG + L+T+AEIG DGKL V VRKS +SR  +             +TPRPSNLT A
Sbjct: 181 VVSLDGHDFLETDAEIGNDGKLHVTVRKSNASRRSLM------------MTPRPSNLTGA 240

Query: 241 EIYSLQSSRNPTPRGSSFNHSDFF--LGNASPRHSNFG---------------NFGNFDE 300
           EIYSL S    TPRGS+FNHSDF+  +G    R SNFG                  NF+E
Sbjct: 241 EIYSLSS----TPRGSNFNHSDFYSVMGFPGGRLSNFGPADLYSVQSSRGPTPRPSNFEE 300

Query: 301 ENGGLKSRGISGNVNGIFPSSNGYPAPASGGLFSPVTG----PAATKKRN---------- 360
            N      G   N N   P++  YPAP     FS  TG    P    K N          
Sbjct: 301 NNA--VKYGFYNNTNSSVPAAGSYPAP--NPEFSTGTGVSTKPNKIPKENQQQLQEKDSK 360

Query: 361 GGDGGKDLHMFVWSSSASPVSEGGINVFRSGDYDGPGAGGMNQKDFNEYGGDEFSF---- 420
                K+LHMFVWSSSASPVS          D  G GAG     + +E G  E       
Sbjct: 361 ASHDAKELHMFVWSSSASPVS----------DVFGGGAGDNVATEQSEQGAKEIRMVVSD 420

Query: 421 -GNKSAVNGGGHDGGPV---------------LSKLGSSSTAELHPKNGDDTAESKPTSM 480
              KS   GGG D G +               L+K+GS+STAEL    GD    +  T M
Sbjct: 421 QPRKSNARGGGDDIGGLDSGEGEREIEKATAGLNKMGSNSTAELEAAGGDG-GGNNGTHM 480

Query: 481 PPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPAIVARSIAILSDAGL 540
           PP SVMTRLILIMVWRKLIRNPNTYSSLIGL W+L+++RW++AMP I+ +SI+ILSDAGL
Sbjct: 481 PPTSVMTRLILIMVWRKLIRNPNTYSSLIGLIWALVAYRWHVAMPKILQQSISILSDAGL 540

Query: 541 GMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIAVGLRGVLLHIAIVQ 597
           GMAMFSLGLFMALQPKIIACGN++A+FAMAVRFITGPA+MA A IA+GL G LL IAIVQ
Sbjct: 541 GMAMFSLGLFMALQPKIIACGNSVATFAMAVRFITGPAIMAVAGIAIGLHGDLLRIAIVQ 600

BLAST of Cmc08g0222891 vs. TAIR 10
Match: AT1G70940.1 (Auxin efflux carrier family protein )

HSP 1 Score: 664.8 bits (1714), Expect = 6.3e-191
Identity = 395/653 (60.49%), Postives = 466/653 (71.36%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MIS  DLY VLTAV+PLYVAMILAYGSV+WWKIFSPDQCSGINRFVA+FAVPLLSFHFIS
Sbjct: 1   MISWHDLYTVLTAVIPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  SNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120
           +NNP++MNLRFIAADTLQK+I+L+ L +W++ +  GSLEWSIT+FSLSTLPNTLVMGIPL
Sbjct: 61  TNNPYAMNLRFIAADTLQKIIMLSLLVLWANFTRSGSLEWSITIFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           L  MYG+ +G+LMVQIVVLQCIIWYTL+LFLFE+RGA++LI EQFP+TA  IVSF+V+SD
Sbjct: 121 LIAMYGEYSGSLMVQIVVLQCIIWYTLLLFLFEFRGAKMLIMEQFPETAASIVSFKVESD 180

Query: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGGVSLTPRPSNLTNA 240
           ++SLDG + L+T+AEIG+DGKL V VRKS +SR             G ++TPRPSNLT A
Sbjct: 181 VVSLDGHDFLETDAEIGDDGKLHVTVRKSNASRRSFC---------GPNMTPRPSNLTGA 240

Query: 241 EIYSLQSSRNPTPRGSSFNHSDFF--LGNASPRHSNFGNFGNFD-EENGGLKSRGISGNV 300
           EIYSL +    TPRGS+FNHSDF+  +G    R SNFG    +  + + G   R  +   
Sbjct: 241 EIYSLST----TPRGSNFNHSDFYNMMGFPGGRLSNFGPADMYSVQSSRGPTPRPSNFEE 300

Query: 301 N---------GIFPSSNGYPAPASGGLFSPVTGPAATKKRN----------------GGD 360
           N         G +P       PA    FS  T   A K  N                GG 
Sbjct: 301 NCAMASSPRFGYYPGGGAGSYPAPNPEFSSTTTSTANKSVNKNPKDVNTNQQTTLPTGGK 360

Query: 361 G----GKDLHMFVWSSSASPVSE-GGINVFRSG-DYDGPGAGGMNQKDF----------- 420
                 K+LHMFVWSS+ SPVS+  G+NVF    D D  G      K+            
Sbjct: 361 SNSHDAKELHMFVWSSNGSPVSDRAGLNVFGGAPDNDQGGRSDQGAKEIRMLVPDQSHNG 420

Query: 421 ----------NEYGGD-EFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKNGDDTAE- 480
                      ++GG+ +FSF  K        D    L+KL  +STA L  K G   AE 
Sbjct: 421 ETKAVAHPASGDFGGEQQFSFAGKEEEAERPKDAENGLNKLAPNSTAALQSKTGLGGAEA 480

Query: 481 SKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMPAIVARSIAI 540
           S+  +MPPASVMTRLILIMVWRKLIRNPNTYSSLIGL W+L++FRW++AMP I+ +SI+I
Sbjct: 481 SQRKNMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLIWALVAFRWHVAMPKIIQQSISI 540

Query: 541 LSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASIAVGLRGVLL 597
           LSDAGLGMAMFSLGLFMALQPK+IACGN++A+FAMAVRF+TGPAVMA A+IA+GLRG LL
Sbjct: 541 LSDAGLGMAMFSLGLFMALQPKLIACGNSVATFAMAVRFLTGPAVMAVAAIAIGLRGDLL 600

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008455726.10.0e+00100.00PREDICTED: probable auxin efflux carrier component 1b [Cucumis melo][more]
XP_004144328.10.0e+0098.83probable auxin efflux carrier component 1b [Cucumis sativus] >KGN54700.1 hypothe... [more]
BAJ10464.10.0e+0098.66auxin efflux facilitator [Cucumis sativus][more]
KAA0025909.10.0e+00100.00putative auxin efflux carrier component 1b [Cucumis melo var. makuwa] >TYK27803.... [more]
XP_038880940.10.0e+0096.34probable auxin efflux carrier component 1b isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q0IQA51.5e-20568.80Probable auxin efflux carrier component 1d OS=Oryza sativa subsp. japonica OX=39... [more]
Q67UL34.4e-20567.32Probable auxin efflux carrier component 1c OS=Oryza sativa subsp. japonica OX=39... [more]
Q9C6B89.8e-20564.55Auxin efflux carrier component 1 OS=Arabidopsis thaliana OX=3702 GN=PIN1 PE=1 SV... [more]
P0C0X52.2e-20468.46Probable auxin efflux carrier component 1b OS=Oryza sativa subsp. japonica OX=39... [more]
Q5SMQ91.0e-20165.69Auxin efflux carrier component 1a OS=Oryza sativa subsp. japonica OX=39947 GN=PI... [more]
Match NameE-valueIdentityDescription
A0A1S3C1I40.0e+00100.00Auxin efflux carrier component OS=Cucumis melo OX=3656 GN=LOC103495829 PE=3 SV=1[more]
A0A0A0KYF40.0e+0098.83Auxin efflux carrier component OS=Cucumis sativus OX=3659 GN=Csa_4G430820 PE=3 S... [more]
D7URM40.0e+0098.66Auxin efflux carrier component OS=Cucumis sativus OX=3659 GN=CsPIN4 PE=2 SV=1[more]
A0A5A7SKX80.0e+00100.00Auxin efflux carrier component OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A6J1HQV84.9e-30893.83Auxin efflux carrier component OS=Cucurbita maxima OX=3661 GN=LOC111465879 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT1G73590.16.9e-20664.55Auxin efflux carrier family protein [more]
AT1G23080.38.5e-19662.91Auxin efflux carrier family protein [more]
AT1G23080.11.4e-19562.52Auxin efflux carrier family protein [more]
AT2G01420.23.3e-19261.51Auxin efflux carrier family protein [more]
AT1G70940.16.3e-19160.49Auxin efflux carrier family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004776Membrane transport proteinPFAMPF03547Mem_transcoord: 9..591
e-value: 3.3E-188
score: 624.9
IPR014024Auxin efflux carrier, plant typeTIGRFAMTIGR00946TIGR00946coord: 1..596
e-value: 4.0E-160
score: 530.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 396..430
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 215..235
NoneNo IPR availablePANTHERPTHR31752:SF43AUXIN EFFLUX CARRIER COMPONENTcoord: 1..596
NoneNo IPR availablePANTHERPTHR31752AUXIN EFFLUX CARRIER COMPONENT 1B-RELATEDcoord: 1..596

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc08g0222891.1Cmc08g0222891.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010315 auxin efflux
biological_process GO:0010252 auxin homeostasis
biological_process GO:0009926 auxin polar transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0010329 auxin efflux transmembrane transporter activity