Cmc08g0221631 (gene) Melon (Charmono) v1.1

Overview
NameCmc08g0221631
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionL-galactono-1,4-lactone dehydrogenase
LocationCMiso1.1chr08: 9524080 .. 9529244 (+)
RNA-Seq ExpressionCmc08g0221631
SyntenyCmc08g0221631
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAACACTAATAATCTCAAGTTTTTGGGCAGTCCGTTCAATTAAGTATTTTAACTCGTCATCTCCCAACTTCGGATTTTGAAGTTCAAACAGCTCAATCTAATTCCGTTGTTGGGTTGGGGAATCCATAAACCCAAGAATCCATCTCAAACCAATTATCAAATGCTCAACTTTCTCTCTCTTCGGCGATCCCTCCATTACTTCCGCCACCGCCGTCTCCAAATCTCACCCACCGCCGCCATTTCCAAACCCCCTTTGAATTCCCCTCGCCCATTTTGCACTGTTTCTCCTTCACCTTCCTCCTCCTTCGATTCCGAGCTTCGTAAGTACCTCGGCTATGGTGCTCTCGTCATTTTCTGCGGCGCCGCCACTTACTACTCCTTCCCTTTTCCCGAAAACGCGAAGCACAAGAAGGCCCAGATCTTCCGATACGCTCCTCTGCCTGAGGATCTTCACACTGTGTCCAATTGGAGTGGAACTCATGAGGTTTTGACCAGAGTGTTTCACCAGCCGGAGGATTTGGAGCAGCTTGAGCAAGTGGTGAAACAGGCTAATGAGAAGAAGGCTAGGGTTCGGCCTGTTGGCTCCGGGCTTTCGCCTAATGGGATTGGATTATCGAGGTTGGGTATGGTGAATATGGCGTTGATGGATAAAGTTTTGGAAGTAGATAAGGAGAAGAAGAGGGTTAGGGTTCAAGCGGGAATAAGGGTTCAGCAGCTTGTGGATGGAATCAAGGAGTATGGGCTTACTTTGCAGAATTTTGCTTCTATCAGGGAGCAGCAGATTGGTGGCATCATTCAGGTGGTTATATGAGCATTTTTGTTTTTTTCATGTTGTTCAATTGCTAGTAGAATTTTGATCTGTTTGAAAACTTGAGCATCAATGTCATGGCTGCGTGTTGATAAATATGAACAATCACCTGTCCAGGGTGTACATTAGTGTTTTGAGTGGAGAAAAAGAATACCATTGATTTCTTATCTTAAATAAATTGAAAGTGATTAGATTTGATATGCAAACTTCCGTTCGATCTTGATTGGCTTGTATTTGGTATCTCTTCTAGTGCCCAATTGTCAAGGATATGATTTTTGAAGGCAGATCGAGGTGTAATAGTGTTAGGAGGTTACATTCGAAACAAAATTTATCTCGTTTGAAGGAGAAATGATCACATTGTTACTCCTAATGATATGAAATGAACATTAATTGCGTAGTTTGGCTATTTCTTTCAGATTTTACTTTGTTTGATTGGAGTCCCTTGTTCTTTTAGTTTGGCTTTTTTGGTTGGTTGGGACTTTATTTTGTATCTCCCTCATACTCTTTCGTTTGTTCTTTATAAACAAAAAAGTAACTACTGTTTTAGGTGGCATAACTATTATATATGGAATACGATGGAAGTTTTCAATTTCAATTTCTATCTTTTCTCTTGGCTTTTTTGTTTATGGACACTTTTCACAATTATGCATCTAGGTTGGTGCACATGGTACCGGGGCAAAATTGCCTCCTATTGATGAGCAGGTTATTGCTATGAAATTGGTCACGCCTGCCAAGGGTACTATAGAGATTTCCAACGATAAAGATCCAGAGCTTTTTTTTTTAGCTCGCTGTGGTCTTGGGGGGCTTGGAGTGGTTGCCGAAGTAACCCTCCAATGTGTTGAGAGACAGGAACTCGTGGAGCACACATATATTTCGAACATGAAAGACATCAAGAAGAACCACAAGTAATTGCATACTTTTTGATAGTTAATTTTATGCAGTTCTGTAGTATGTTAGAGACTTCAGTTTACATCTCATCTTATTTATTCTTGTTATTATTTGACAATGTTTAGAGGTGAATTGAAATATTTAGGTCCCAGTATTCTGATAATCTAAAAATTAAATACTAAATTTGAGTTATTTTGTCGCTAAAACATCTATACTTAGTTGTAGATCCCTATAGAGGTGGATGCTCCCATCTTTCATGCTTTTGTGAAACAAGCATCCCATATCTTAGTGAGAGAATTACCAATTTGTGAAAGTTGAATTTTGCCATTTCTTAAACAAAAATATATTTTGAGCAGAATTAATGGATATAAATGTAATTTTCTAATCTCTAAAAGGAAACTTTCTATAGTTTGAATTTTGGGCTCTTCAAGAATGTATCATGTTGGGAATTCTAATGGATAGAAAGTGAGATTCTGATTCAGGTGTTTTCCACTTTTTTAACCTTTCGTACCTTAATGTAGAGCCTTTCTTTTTTTCCTCCTCCCTGAAAAACCAGGACCACAAAAACCCCTCTAGAAAAAAGGGTTTTCCATCTAAGTAAGATTAGAGAATAATTATAAAAAAGTCTTTGAGACCGAAGCCCAACGTTCGAAGCCCTCGACTTGCAAGCCATACTATTTATTGTGTTATAGTATACAAAGGTTGTATATAGATTGAAATCCAAACTATTGATTCTTGGGAGTTGTTGCTTAATGTAGACTTATATTATTAATTTCAAATTGTCTAGTTTTGAACAAGGCTTCTCTTTTGGATCTAAACTAAATTTAATCCTGTATCTCTACCATCCACGTCACTCACAAATAGGATGAAACCTTTTTTATTTCATTTCATTTTTTTGTCTTCATCCTCTAAAGTCAAGTCACCCAAGTAGTGTTGAAGAATTACACATATAAGGTCTGATATTCTCTCTATGCAGGAAGTTATTGGCTGACAATAAGCATGTGAAATACCTGTATATTCCTTACACAGATGCTATTGTTGTTGTAACTTGCAATCCTATCTCAAAATGGAGAGGCCCTCCGAAATTCAAACCGAAATATACAAGTGAGGAAGCCATACAGCATGTTCGTGATCTCTACGTGGAGTCTCTTAAAAAATACAGGTTACTTCCAGCTTCTGTTTGAGTATATGATTTTAGGACGCTGGAAATGGGTCGAAACGTAGTTGACATTTTTATCCCCTCAAATCACTTTTCATTCCTTTTGATTTCTTTCCCCCTTCTCTTTTGGTTGACATTCATTTTTCATCAAAGAGTCATTTGAGGCCTTTTTCAAATTTTTTTGCTATTTATACTTTTCCTCCCCATTGTTTTCCCAGTGCTTTTTATTTCATCTAATTGCTTGCTTTATGATTGCAGTGCTAGTGAGGAAAGAGACATAAATGAATTTTCATTTACTGAGTTGCGCGATAAACTACTTGCCCTTGATCCTCTTAACAAAGAGCATGTCATGAAAGTGAATCAAGCAGAGGCTGAGTTCTGGAGGAAGTCAGAAGGATACAGAGTCGGATGGAGTGATGAAATCTTGGGTTTTGATTGTGGTGGCCAACAATGGGTCTCTGAGACTTGCTTTCCAGCCGGTACTCTTGCAAAACCTAACATGAAAGACATTGAATACATAGAGGAATTGAAGCAACTTATTGAAAAAGAAAACATACCTGCACCTGCTCCTCTTGAGCAGCGGTGGACTGGTCGTAGCAAGAGTCCCATGAGTCCAGCTTCAAGCACAGCTGAGGATGATATATTCTCTTGGGTAAACTCTAAATTTCAGACTTTATACTTGCTCTCTTTCAACTTTTAAATCTATACCTGTGCAGCCCCGAGGGTTGCCCTGGTGATAAAGACAGGGACGTTTAGAGGTATCCTTCAAGATTTTGGAACTTAATTACTTAAAACAATCCTTGTTCTTTTGGAGTTGGTATTGGGGCAGGCGAGCTTCATAACCACTATAATGGAAAGAGTGTCCCCACATCTTGGTTAAAAATTGACTTTCTACGCTTGTGCTATTGTGCATAAAGGGAGAAGCTAAGTTATATTTTAGAGAAATAGTGAAACTAAATATCAAGTTTCTTTTCTGCTAAATGGGTCTTATATTCTGAAGCGTGTTGTGATCTTATATGATGATTAGTTGCTGACGGGAGTCCTCAAATTAATTTTCTTTCAGTAGCTTCCGGTCTTTGCCATCTCAAAAGCACATCGTTTTTTAATTTAGGACACAAACACAACAAGAACATATGTACTAAAGGTTAAAACATACTTCTTTAAAATATTTAAAGTTTTATACTAGTACATCAAAGTCAATAAGTTAGGCATCTATATGCTTAAAATTCAGTAGTTTGCTCTCAATTTTTTGTTTTCTTTTTGTCATTTATGTGTCTGTTTAGTTTTAGTATATGAAACAAATATTTGATGCATGCCTAACAAATGTCAGTACTTAATTGGACTTTGTATAACTAATGTCTAACACATGTATTGCACTAACAAGTGTTAGATACGTGTGCAAGTGTTTGACACATGTTGGATACAGACACATTGGCCAAACTAAAGTGTTACGCCATGTTCTTGTTGCATCAATGTGGTGTTGTGTCACATCCGTATCTGTGCTTCTCAACAGAAATCTTTACCCAATTGTCCACAGATTTTATTTTGATGTCTCACCAATTAGAACTTAGTAGACCCAACTGTGGTTTCTTCTTTGTTAATCTTGTTAATTTGGTTGATTGTCTTTTGCTGTGATTAGGTCGGAATAATCATGTACCTACCTACGTCAGATGCACGCCAGAGAAAGGAAATCACAGAGGAGTTCTTTCACTATAGGCACCTCACCCAAACACTGCTATGGGATAAATTTTCTGCCTTTGAACATTGGGCTAAGATTGAGGTACCCAATTTCAAAATCTTTTCCTGATTAAGCCCTACAAAAATGGGGGCATAAAGTTTTGACCACTTTGTTGTTGTATGTAAAATCCAGGTTCCCAAGGACAAGGATGAGCTTGCAGCCCTGCAAGCAAGACTTAGGAAACGGTTTCCTGTTGACGAATACAACAAGGCACGACGGGCACTGGACCCGAACAAAATTCTTTCAAATAACAAGTTGGAAAAACTGTTCTCGTCATTGGATATGGTTTGAGATGCACACATGTTTTTAGTGATCAATAATAAGTCCTGTTGTCCAGTTGGATAAGTGTTTGTTTTCTGGCTGGTACTGTACATGTTCACAGGAATGGTGTTATTTGTCAATTCCTTTGGGTATATTATTTCTTTGAATTTTTAGGCCATAAAAAGTATTCTTGAGGAAGGAGATGTTTTTCGATGGCTTGTTGATCGCATTTGAAAATGATAATACCAAATTTTGGATCTTTTCGGGATTTGTACATCTTTCTATAGAAATGTTTAGGAAATATTCATAAA

mRNA sequence

TAACACTAATAATCTCAAGTTTTTGGGCAGTCCGTTCAATTAAGTATTTTAACTCGTCATCTCCCAACTTCGGATTTTGAAGTTCAAACAGCTCAATCTAATTCCGTTGTTGGGTTGGGGAATCCATAAACCCAAGAATCCATCTCAAACCAATTATCAAATGCTCAACTTTCTCTCTCTTCGGCGATCCCTCCATTACTTCCGCCACCGCCGTCTCCAAATCTCACCCACCGCCGCCATTTCCAAACCCCCTTTGAATTCCCCTCGCCCATTTTGCACTGTTTCTCCTTCACCTTCCTCCTCCTTCGATTCCGAGCTTCGTAAGTACCTCGGCTATGGTGCTCTCGTCATTTTCTGCGGCGCCGCCACTTACTACTCCTTCCCTTTTCCCGAAAACGCGAAGCACAAGAAGGCCCAGATCTTCCGATACGCTCCTCTGCCTGAGGATCTTCACACTGTGTCCAATTGGAGTGGAACTCATGAGGTTTTGACCAGAGTGTTTCACCAGCCGGAGGATTTGGAGCAGCTTGAGCAAGTGGTGAAACAGGCTAATGAGAAGAAGGCTAGGGTTCGGCCTGTTGGCTCCGGGCTTTCGCCTAATGGGATTGGATTATCGAGGTTGGGTATGGTGAATATGGCGTTGATGGATAAAGTTTTGGAAGTAGATAAGGAGAAGAAGAGGGTTAGGGTTCAAGCGGGAATAAGGGTTCAGCAGCTTGTGGATGGAATCAAGGAGTATGGGCTTACTTTGCAGAATTTTGCTTCTATCAGGGAGCAGCAGATTGGTGGCATCATTCAGGTGGTTGGTGCACATGGTACCGGGGCAAAATTGCCTCCTATTGATGAGCAGGTTATTGCTATGAAATTGGTCACGCCTGCCAAGGGTACTATAGAGATTTCCAACGATAAAGATCCAGAGCTTTTTTTTTTAGCTCGCTGTGGTCTTGGGGGGCTTGGAGTGGTTGCCGAAGTAACCCTCCAATGTGTTGAGAGACAGGAACTCGTGGAGCACACATATATTTCGAACATGAAAGACATCAAGAAGAACCACAAGAAGTTATTGGCTGACAATAAGCATGTGAAATACCTGTATATTCCTTACACAGATGCTATTGTTGTTGTAACTTGCAATCCTATCTCAAAATGGAGAGGCCCTCCGAAATTCAAACCGAAATATACAAGTGAGGAAGCCATACAGCATGTTCGTGATCTCTACGTGGAGTCTCTTAAAAAATACAGTGCTAGTGAGGAAAGAGACATAAATGAATTTTCATTTACTGAGTTGCGCGATAAACTACTTGCCCTTGATCCTCTTAACAAAGAGCATGTCATGAAAGTGAATCAAGCAGAGGCTGAGTTCTGGAGGAAGTCAGAAGGATACAGAGTCGGATGGAGTGATGAAATCTTGGGTTTTGATTGTGGTGGCCAACAATGGGTCTCTGAGACTTGCTTTCCAGCCGGTACTCTTGCAAAACCTAACATGAAAGACATTGAATACATAGAGGAATTGAAGCAACTTATTGAAAAAGAAAACATACCTGCACCTGCTCCTCTTGAGCAGCGGTGGACTGGTCGTAGCAAGAGTCCCATGAGTCCAGCTTCAAGCACAGCTGAGGATGATATATTCTCTTGGGTCGGAATAATCATGTACCTACCTACGTCAGATGCACGCCAGAGAAAGGAAATCACAGAGGAGTTCTTTCACTATAGGCACCTCACCCAAACACTGCTATGGGATAAATTTTCTGCCTTTGAACATTGGGCTAAGATTGAGGTTCCCAAGGACAAGGATGAGCTTGCAGCCCTGCAAGCAAGACTTAGGAAACGGTTTCCTGTTGACGAATACAACAAGGCACGACGGGCACTGGACCCGAACAAAATTCTTTCAAATAACAAGTTGGAAAAACTGTTCTCGTCATTGGATATGGTTTGAGATGCACACATGTTTTTAGTGATCAATAATAAGTCCTGTTGTCCAGTTGGATAAGTGTTTGTTTTCTGGCTGGTACTGTACATGTTCACAGGAATGGTGTTATTTGTCAATTCCTTTGGGTATATTATTTCTTTGAATTTTTAGGCCATAAAAAGTATTCTTGAGGAAGGAGATGTTTTTCGATGGCTTGTTGATCGCATTTGAAAATGATAATACCAAATTTTGGATCTTTTCGGGATTTGTACATCTTTCTATAGAAATGTTTAGGAAATATTCATAAA

Coding sequence (CDS)

ATGCTCAACTTTCTCTCTCTTCGGCGATCCCTCCATTACTTCCGCCACCGCCGTCTCCAAATCTCACCCACCGCCGCCATTTCCAAACCCCCTTTGAATTCCCCTCGCCCATTTTGCACTGTTTCTCCTTCACCTTCCTCCTCCTTCGATTCCGAGCTTCGTAAGTACCTCGGCTATGGTGCTCTCGTCATTTTCTGCGGCGCCGCCACTTACTACTCCTTCCCTTTTCCCGAAAACGCGAAGCACAAGAAGGCCCAGATCTTCCGATACGCTCCTCTGCCTGAGGATCTTCACACTGTGTCCAATTGGAGTGGAACTCATGAGGTTTTGACCAGAGTGTTTCACCAGCCGGAGGATTTGGAGCAGCTTGAGCAAGTGGTGAAACAGGCTAATGAGAAGAAGGCTAGGGTTCGGCCTGTTGGCTCCGGGCTTTCGCCTAATGGGATTGGATTATCGAGGTTGGGTATGGTGAATATGGCGTTGATGGATAAAGTTTTGGAAGTAGATAAGGAGAAGAAGAGGGTTAGGGTTCAAGCGGGAATAAGGGTTCAGCAGCTTGTGGATGGAATCAAGGAGTATGGGCTTACTTTGCAGAATTTTGCTTCTATCAGGGAGCAGCAGATTGGTGGCATCATTCAGGTGGTTGGTGCACATGGTACCGGGGCAAAATTGCCTCCTATTGATGAGCAGGTTATTGCTATGAAATTGGTCACGCCTGCCAAGGGTACTATAGAGATTTCCAACGATAAAGATCCAGAGCTTTTTTTTTTAGCTCGCTGTGGTCTTGGGGGGCTTGGAGTGGTTGCCGAAGTAACCCTCCAATGTGTTGAGAGACAGGAACTCGTGGAGCACACATATATTTCGAACATGAAAGACATCAAGAAGAACCACAAGAAGTTATTGGCTGACAATAAGCATGTGAAATACCTGTATATTCCTTACACAGATGCTATTGTTGTTGTAACTTGCAATCCTATCTCAAAATGGAGAGGCCCTCCGAAATTCAAACCGAAATATACAAGTGAGGAAGCCATACAGCATGTTCGTGATCTCTACGTGGAGTCTCTTAAAAAATACAGTGCTAGTGAGGAAAGAGACATAAATGAATTTTCATTTACTGAGTTGCGCGATAAACTACTTGCCCTTGATCCTCTTAACAAAGAGCATGTCATGAAAGTGAATCAAGCAGAGGCTGAGTTCTGGAGGAAGTCAGAAGGATACAGAGTCGGATGGAGTGATGAAATCTTGGGTTTTGATTGTGGTGGCCAACAATGGGTCTCTGAGACTTGCTTTCCAGCCGGTACTCTTGCAAAACCTAACATGAAAGACATTGAATACATAGAGGAATTGAAGCAACTTATTGAAAAAGAAAACATACCTGCACCTGCTCCTCTTGAGCAGCGGTGGACTGGTCGTAGCAAGAGTCCCATGAGTCCAGCTTCAAGCACAGCTGAGGATGATATATTCTCTTGGGTCGGAATAATCATGTACCTACCTACGTCAGATGCACGCCAGAGAAAGGAAATCACAGAGGAGTTCTTTCACTATAGGCACCTCACCCAAACACTGCTATGGGATAAATTTTCTGCCTTTGAACATTGGGCTAAGATTGAGGTTCCCAAGGACAAGGATGAGCTTGCAGCCCTGCAAGCAAGACTTAGGAAACGGTTTCCTGTTGACGAATACAACAAGGCACGACGGGCACTGGACCCGAACAAAATTCTTTCAAATAACAAGTTGGAAAAACTGTTCTCGTCATTGGATATGGTTTGA

Protein sequence

MLNFLSLRRSLHYFRHRRLQISPTAAISKPPLNSPRPFCTVSPSPSSSFDSELRKYLGYGALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPEDLEQLEQVVKQANEKKARVRPVGSGLSPNGIGLSRLGMVNMALMDKVLEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVVGAHGTGAKLPPIDEQVIAMKLVTPAKGTIEISNDKDPELFFLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYSASEERDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTGRSKSPMSPASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDKFSAFEHWAKIEVPKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKLFSSLDMV
Homology
BLAST of Cmc08g0221631 vs. NCBI nr
Match: NP_001284400.1 (L-galactono-1,4-lactone dehydrogenase, mitochondrial [Cucumis melo] >AAF64319.2 L-galactono-1,4-lactone dehydrogenase [Cucumis melo])

HSP 1 Score: 1176.4 bits (3042), Expect = 0.0e+00
Identity = 587/590 (99.49%), Postives = 588/590 (99.66%), Query Frame = 0

Query: 1   MLNFLSLRRSLHYFRHRRLQISPTAAISKPPLNSPRPFCTVSPSPSSSFDSELRKYLGYG 60
           MLNFLSLRRSLHYFRHRRLQISPTAAISKPPLNSPRPFCTVSPSPSSSFDSELRKYLGYG
Sbjct: 1   MLNFLSLRRSLHYFRHRRLQISPTAAISKPPLNSPRPFCTVSPSPSSSFDSELRKYLGYG 60

Query: 61  ALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPEDL 120
           ALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPEDL
Sbjct: 61  ALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPEDL 120

Query: 121 EQLEQVVKQANEKKARVRPVGSGLSPNGIGLSRLGMVNMALMDKVLEVDKEKKRVRVQAG 180
           EQLEQVVKQANEKKARVRPVGSGLSPNGIGLSRLGMVNMALMDKVLEVDKEKKRVRVQAG
Sbjct: 121 EQLEQVVKQANEKKARVRPVGSGLSPNGIGLSRLGMVNMALMDKVLEVDKEKKRVRVQAG 180

Query: 181 IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVVGAHGTGAKLPPIDEQVIAMKLVTPA 240
           IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQ VGAHGTGAKLPPIDEQVIAMKLVTPA
Sbjct: 181 IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQ-VGAHGTGAKLPPIDEQVIAMKLVTPA 240

Query: 241 KGTIEISNDKDPELFFLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL 300
           KGTIEISNDKDPELFFLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL
Sbjct: 241 KGTIEISNDKDPELFFLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL 300

Query: 301 LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYS 360
           LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYS
Sbjct: 301 LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYS 360

Query: 361 ASEERDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDC 420
           ASEERDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGY+VGWSDEILGFDC
Sbjct: 361 ASEERDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYKVGWSDEILGFDC 420

Query: 421 GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTGRSKSPMSPA 480
           GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTGRSKSPMSPA
Sbjct: 421 GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTGRSKSPMSPA 480

Query: 481 SSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDKFSAFEHWAKIEVP 540
           SSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDKFSAFEHWAKIEVP
Sbjct: 481 SSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDKFSAFEHWAKIEVP 540

Query: 541 KDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKLFSSLDMV 591
           KDKDELAALQARLRKRFPVDE NKARRALDPNKILSNNKLEKLFSSLDMV
Sbjct: 541 KDKDELAALQARLRKRFPVDESNKARRALDPNKILSNNKLEKLFSSLDMV 589

BLAST of Cmc08g0221631 vs. NCBI nr
Match: NP_001292704.1 (L-galactono-1,4-lactone dehydrogenase, mitochondrial [Cucumis sativus] >ADT80722.1 L-galactono-1,4-lactone dehydrogenase [Cucumis sativus])

HSP 1 Score: 1141.3 bits (2951), Expect = 0.0e+00
Identity = 569/591 (96.28%), Postives = 579/591 (97.97%), Query Frame = 0

Query: 1   MLNFLSLRRSLHYFRHRRLQISPTAAISKPPLNSPRPFCTVSPSP-SSSFDSELRKYLGY 60
           MLNFLSLRRS+HYFRHRRLQISPTAAISKPP N PRPF TVSPSP SSSFDSELRKYLGY
Sbjct: 1   MLNFLSLRRSIHYFRHRRLQISPTAAISKPPFNPPRPFSTVSPSPSSSSFDSELRKYLGY 60

Query: 61  GALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPED 120
           GALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEV TRVFHQPE+
Sbjct: 61  GALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVWTRVFHQPEN 120

Query: 121 LEQLEQVVKQANEKKARVRPVGSGLSPNGIGLSRLGMVNMALMDKVLEVDKEKKRVRVQA 180
           LEQLEQVVKQANEKKAR+RPVGSGLSPNGIGLSR+GMVN+ALMDKVLEVDKEKKRVRVQA
Sbjct: 121 LEQLEQVVKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQA 180

Query: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVVGAHGTGAKLPPIDEQVIAMKLVTP 240
           GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQ VGAHGTGAKLPPIDEQVIAMKLVTP
Sbjct: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQ-VGAHGTGAKLPPIDEQVIAMKLVTP 240

Query: 241 AKGTIEISNDKDPELFFLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKK 300
           AKGTIEIS DKDPELF LARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKK
Sbjct: 241 AKGTIEISKDKDPELFLLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKK 300

Query: 301 LLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKY 360
           LLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKY
Sbjct: 301 LLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKY 360

Query: 361 SASEERDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFD 420
           SASEERD+NE SFTELRDKLLALDPLNKEHV+KVNQAEAEFWRKSEGYRVGWSDEILGFD
Sbjct: 361 SASEERDMNEISFTELRDKLLALDPLNKEHVIKVNQAEAEFWRKSEGYRVGWSDEILGFD 420

Query: 421 CGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTGRSKSPMSP 480
           CGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEK+NIPAPAPLEQRWT RSKSPMSP
Sbjct: 421 CGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKKNIPAPAPLEQRWTARSKSPMSP 480

Query: 481 ASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDKFSAFEHWAKIEV 540
           ASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWD++SAFEHWAKIEV
Sbjct: 481 ASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEV 540

Query: 541 PKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKLFSSLDMV 591
           PKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKLFSS D V
Sbjct: 541 PKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKLFSSTDTV 590

BLAST of Cmc08g0221631 vs. NCBI nr
Match: KAE8637410.1 (hypothetical protein CSA_023387 [Cucumis sativus])

HSP 1 Score: 1131.7 bits (2926), Expect = 0.0e+00
Identity = 563/584 (96.40%), Postives = 573/584 (98.12%), Query Frame = 0

Query: 8   RRSLHYFRHRRLQISPTAAISKPPLNSPRPFCTVSPSP-SSSFDSELRKYLGYGALVIFC 67
           RRS+HYFRHRRLQISPTAAISKPP N PRPF TVSPSP SSSFDSELRKYLGYGALVIFC
Sbjct: 240 RRSIHYFRHRRLQISPTAAISKPPFNPPRPFSTVSPSPSSSSFDSELRKYLGYGALVIFC 299

Query: 68  GAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPEDLEQLEQV 127
           GAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEV TRVFHQPE+LEQLEQV
Sbjct: 300 GAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVWTRVFHQPENLEQLEQV 359

Query: 128 VKQANEKKARVRPVGSGLSPNGIGLSRLGMVNMALMDKVLEVDKEKKRVRVQAGIRVQQL 187
           VKQANEKKAR+RPVGSGLSPNGIGLSR+GMVN+ALMDKVLEVDKEKKRVRVQAGIRVQQL
Sbjct: 360 VKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQAGIRVQQL 419

Query: 188 VDGIKEYGLTLQNFASIREQQIGGIIQVVGAHGTGAKLPPIDEQVIAMKLVTPAKGTIEI 247
           VDGIKEYGLTLQNFASIREQQIGGIIQ VGAHGTGAKLPPIDEQVIAMKLVTPAKGTIEI
Sbjct: 420 VDGIKEYGLTLQNFASIREQQIGGIIQ-VGAHGTGAKLPPIDEQVIAMKLVTPAKGTIEI 479

Query: 248 SNDKDPELFFLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLLADNKH 307
           S DKDPELF LARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLLADNKH
Sbjct: 480 SKDKDPELFLLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLLADNKH 539

Query: 308 VKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYSASEERD 367
           VKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYSASEERD
Sbjct: 540 VKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYSASEERD 599

Query: 368 INEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWV 427
           +NEFSFTELRDKLLALDPLNKEHV+KVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWV
Sbjct: 600 MNEFSFTELRDKLLALDPLNKEHVIKVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWV 659

Query: 428 SETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTGRSKSPMSPASSTAED 487
           SETCFPAGTLAKPNMKDIEYIEELKQLIEK+NIPAPAPLEQRWT RSKSPMSPASSTAED
Sbjct: 660 SETCFPAGTLAKPNMKDIEYIEELKQLIEKKNIPAPAPLEQRWTARSKSPMSPASSTAED 719

Query: 488 DIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDKFSAFEHWAKIEVPKDKDEL 547
           DIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWD++SAFEHWAKIEVPKDKDEL
Sbjct: 720 DIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPKDKDEL 779

Query: 548 AALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKLFSSLDMV 591
           AALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKLFSS D V
Sbjct: 780 AALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKLFSSTDTV 822

BLAST of Cmc08g0221631 vs. NCBI nr
Match: XP_038882838.1 (L-galactono-1,4-lactone dehydrogenase, mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 1092.8 bits (2825), Expect = 0.0e+00
Identity = 547/591 (92.55%), Postives = 564/591 (95.43%), Query Frame = 0

Query: 1   MLNFLSLRRSLHYFRHRRLQISPTAAISKPPLNSPRPFCTVSPSP-SSSFDSELRKYLGY 60
           ML  L+LRRS H+ R R LQI PT AISKPPLN PRPF TVSP P SSSFDSELRKYLGY
Sbjct: 1   MLKLLALRRS-HFCRLRGLQIPPTTAISKPPLNPPRPFSTVSPPPQSSSFDSELRKYLGY 60

Query: 61  GALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPED 120
           G LVI CGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEV TRVFHQPE+
Sbjct: 61  GTLVIICGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVQTRVFHQPEN 120

Query: 121 LEQLEQVVKQANEKKARVRPVGSGLSPNGIGLSRLGMVNMALMDKVLEVDKEKKRVRVQA 180
           LEQLEQVVK+ANEKKAR+RPVGSGLSPNGIGLSR+GMVN+ALMD VLEVDK++KRVRVQA
Sbjct: 121 LEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDNVLEVDKDRKRVRVQA 180

Query: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVVGAHGTGAKLPPIDEQVIAMKLVTP 240
           GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQ VGAHGTGAKLPPIDEQVIAMKLVTP
Sbjct: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQ-VGAHGTGAKLPPIDEQVIAMKLVTP 240

Query: 241 AKGTIEISNDKDPELFFLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKK 300
           AKGTIEIS DKDPELFFLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKK
Sbjct: 241 AKGTIEISKDKDPELFFLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKK 300

Query: 301 LLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKY 360
           LLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPK KPKYTSEEAIQHVRDLYVESLKK+
Sbjct: 301 LLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKLKPKYTSEEAIQHVRDLYVESLKKH 360

Query: 361 SASEERDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFD 420
           S S+E DINEFSFTELRDKLLALDPLNKEHV+KVNQAEAEFW+KSEGYRVGWSDEILGFD
Sbjct: 361 SVSDETDINEFSFTELRDKLLALDPLNKEHVIKVNQAEAEFWKKSEGYRVGWSDEILGFD 420

Query: 421 CGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTGRSKSPMSP 480
           CGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAP+EQRWT  SKSPMSP
Sbjct: 421 CGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSP 480

Query: 481 ASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDKFSAFEHWAKIEV 540
           ASS+AEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHL QTLLWD++SAFEHWAKIEV
Sbjct: 481 ASSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLAQTLLWDQYSAFEHWAKIEV 540

Query: 541 PKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKLFSSLDMV 591
           PKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKL SS D V
Sbjct: 541 PKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKLLSSSDTV 589

BLAST of Cmc08g0221631 vs. NCBI nr
Match: XP_023517263.1 (L-galactono-1,4-lactone dehydrogenase, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1088.2 bits (2813), Expect = 0.0e+00
Identity = 545/591 (92.22%), Postives = 565/591 (95.60%), Query Frame = 0

Query: 1   MLNFLSLRRSLHYFRHRRLQISPTAAISKPPLNSPRPFCTVS-PSPSSSFDSELRKYLGY 60
           ML  LSLRRSLH   HRR Q  PT AISKPPLN  R F TVS P PSSS +SELRKYLGY
Sbjct: 1   MLKLLSLRRSLH-LHHRRPQFPPTVAISKPPLNPTRQFSTVSPPPPSSSSESELRKYLGY 60

Query: 61  GALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPED 120
           GALVI CGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPE+
Sbjct: 61  GALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPEN 120

Query: 121 LEQLEQVVKQANEKKARVRPVGSGLSPNGIGLSRLGMVNMALMDKVLEVDKEKKRVRVQA 180
           LEQLE+VVK++N KKAR+RPVGSGLSPNGIGLSR+GMVN+ALMD+VLEVDKEKKRVRVQA
Sbjct: 121 LEQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDRVLEVDKEKKRVRVQA 180

Query: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVVGAHGTGAKLPPIDEQVIAMKLVTP 240
           GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQ VGAHGTGAKLPPIDEQVIAMKLVTP
Sbjct: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQ-VGAHGTGAKLPPIDEQVIAMKLVTP 240

Query: 241 AKGTIEISNDKDPELFFLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKK 300
           AKGTIEIS DKDPELF+LARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKK
Sbjct: 241 AKGTIEISKDKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKK 300

Query: 301 LLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKY 360
           LLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKF+PKYTS+EAIQHVRDLYVESLKKY
Sbjct: 301 LLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLKKY 360

Query: 361 SASEERDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFD 420
           SAS+ERDINEFSFTELRDKLLALDPLNKEHV+KVNQAEAEFWRKSEGYRVGWSDEILGFD
Sbjct: 361 SASDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWRKSEGYRVGWSDEILGFD 420

Query: 421 CGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTGRSKSPMSP 480
           CGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAP+EQRWT  SKSPMSP
Sbjct: 421 CGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSP 480

Query: 481 ASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDKFSAFEHWAKIEV 540
           ASS+AEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWD++SAFEHWAKIEV
Sbjct: 481 ASSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEV 540

Query: 541 PKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKLFSSLDMV 591
           PKDKDELAALQARLRKRFPVDEYNKAR  LDPNKILS+NKLEKLF S D+V
Sbjct: 541 PKDKDELAALQARLRKRFPVDEYNKARGELDPNKILSSNKLEKLFVSSDVV 589

BLAST of Cmc08g0221631 vs. ExPASy Swiss-Prot
Match: Q9SU56 (L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=GLDH PE=1 SV=1)

HSP 1 Score: 918.3 bits (2372), Expect = 4.4e-266
Identity = 459/608 (75.49%), Postives = 513/608 (84.38%), Query Frame = 0

Query: 1   MLNFLSLRRSLHY---------------FRHRRLQISPTAAISKPPLNSPRPFCTVSPSP 60
           ML  L LRRS+ +               F   R   +    ++ PP   PRP     P P
Sbjct: 1   MLRSLLLRRSVGHSLGTLSPSSSTIRSSFSPHRTLCTTGQTLTPPPPPPPRP---PPPPP 60

Query: 61  SSSFDSELRKYLGYGALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSG 120
           +++ +++ RKY GY AL IF G ATY+SFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSG
Sbjct: 61  ATASEAQFRKYAGYAALAIFSGVATYFSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSG 120

Query: 121 THEVLTRVFHQPEDLEQLEQVVKQANEKKARVRPVGSGLSPNGIGLSRLGMVNMALMDKV 180
           THEV TR F+QPE+L  LE +VK+++EKK R+RPVGSGLSPNGIGLSR GMVN+ALMDKV
Sbjct: 121 THEVQTRNFNQPENLADLEALVKESHEKKLRIRPVGSGLSPNGIGLSRSGMVNLALMDKV 180

Query: 181 LEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVVGAHGTGAKLP 240
           LEVDKEKKRV VQAGIRVQQLVD IK+YGLTLQNFASIREQQIGGIIQ VGAHGTGA+LP
Sbjct: 181 LEVDKEKKRVTVQAGIRVQQLVDAIKDYGLTLQNFASIREQQIGGIIQ-VGAHGTGARLP 240

Query: 241 PIDEQVIAMKLVTPAKGTIEISNDKDPELFFLARCGLGGLGVVAEVTLQCVERQELVEHT 300
           PIDEQVI+MKLVTPAKGTIE+S +KDPELF LARCGLGGLGVVAEVTLQCV R ELVEHT
Sbjct: 241 PIDEQVISMKLVTPAKGTIELSREKDPELFHLARCGLGGLGVVAEVTLQCVARHELVEHT 300

Query: 301 YISNMKDIKKNHKKLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAI 360
           Y+SN+++IKKNHKKLL+ NKHVKYLYIPYTD +VVVTCNP+SKW GPPK KPKYT++EA+
Sbjct: 301 YVSNLQEIKKNHKKLLSANKHVKYLYIPYTDTVVVVTCNPVSKWSGPPKDKPKYTTDEAV 360

Query: 361 QHVRDLYVESLKKYSA---------SEERDINEFSFTELRDKLLALDPLNKEHVMKVNQA 420
           QHVRDLY ES+ KY           S E DI E SFTELRDKLLALDPLN  HV KVNQA
Sbjct: 361 QHVRDLYRESIVKYRVQDSGKKSPDSSEPDIQELSFTELRDKLLALDPLNDVHVAKVNQA 420

Query: 421 EAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEK 480
           EAEFW+KSEGYRVGWSDEILGFDCGGQQWVSE+CFPAGTLA P+MKD+EYIEELK+LIEK
Sbjct: 421 EAEFWKKSEGYRVGWSDEILGFDCGGQQWVSESCFPAGTLANPSMKDLEYIEELKKLIEK 480

Query: 481 ENIPAPAPLEQRWTGRSKSPMSPASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHY 540
           E IPAPAP+EQRWT RSKSP+SPA ST+EDDIFSWVGIIMYLPT+D RQRK+IT+EFFHY
Sbjct: 481 EAIPAPAPIEQRWTARSKSPISPAFSTSEDDIFSWVGIIMYLPTADPRQRKDITDEFFHY 540

Query: 541 RHLTQTLLWDKFSAFEHWAKIEVPKDKDELAALQARLRKRFPVDEYNKARRALDPNKILS 585
           RHLTQ  LWD+FSA+EHWAKIE+PKDK+EL ALQAR+RKRFPVD YNKARR LDPN+ILS
Sbjct: 541 RHLTQKQLWDQFSAYEHWAKIEIPKDKEELEALQARIRKRFPVDAYNKARRELDPNRILS 600

BLAST of Cmc08g0221631 vs. ExPASy Swiss-Prot
Match: O47881 (L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Brassica oleracea OX=3712 PE=1 SV=1)

HSP 1 Score: 917.5 bits (2370), Expect = 7.5e-266
Identity = 462/608 (75.99%), Postives = 511/608 (84.05%), Query Frame = 0

Query: 1   MLNFLSLRRSLHYFRHRRLQISPTAAISKPPLNSPRPFC----TVSPSP----------- 60
           ML  L LRRS              A   +PP    R  C    T++P+P           
Sbjct: 1   MLRSLLLRRS-------------NARSLRPPFPPLRTLCTSGQTLTPAPPPPPPPPPPIS 60

Query: 61  SSSFDSELRKYLGYGALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSG 120
           SS+ + E RKY GY AL +F GAATY+SFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSG
Sbjct: 61  SSASEKEFRKYAGYAALALFSGAATYFSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSG 120

Query: 121 THEVLTRVFHQPEDLEQLEQVVKQANEKKARVRPVGSGLSPNGIGLSRLGMVNMALMDKV 180
           THEV TR F+QPE L  LE +VK+A+EKK R+RPVGSGLSPNGIGLSR GMVN+ALMDKV
Sbjct: 121 THEVQTRNFNQPETLADLEALVKEAHEKKNRIRPVGSGLSPNGIGLSRSGMVNLALMDKV 180

Query: 181 LEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVVGAHGTGAKLP 240
           LEVDKEKKRVRVQAGIRVQQLVD I+EYGLTLQNFASIREQQIGGIIQ VGAHGTGA+LP
Sbjct: 181 LEVDKEKKRVRVQAGIRVQQLVDAIQEYGLTLQNFASIREQQIGGIIQ-VGAHGTGARLP 240

Query: 241 PIDEQVIAMKLVTPAKGTIEISNDKDPELFFLARCGLGGLGVVAEVTLQCVERQELVEHT 300
           PIDEQVI MKLVTPAKGTIE+S D DPELF LARCGLGGLGVVAEVTLQCVERQEL+EHT
Sbjct: 241 PIDEQVIGMKLVTPAKGTIELSKDNDPELFHLARCGLGGLGVVAEVTLQCVERQELLEHT 300

Query: 301 YISNMKDIKKNHKKLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAI 360
           Y+S +++IKKNHKKLL+ NKHVKYLYIPYTD +VVVTCNP+SKW G PK KPKYT+EEA+
Sbjct: 301 YVSTLEEIKKNHKKLLSTNKHVKYLYIPYTDTVVVVTCNPVSKWSGAPKDKPKYTTEEAL 360

Query: 361 QHVRDLYVESLKKYSA---------SEERDINEFSFTELRDKLLALDPLNKEHVMKVNQA 420
           +HVRDLY ES+ KY           S E DINE SFTELRDKL+ALDPLN  HV KVNQA
Sbjct: 361 KHVRDLYRESIVKYRVQDSSKKTPDSREPDINELSFTELRDKLIALDPLNDVHVGKVNQA 420

Query: 421 EAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEK 480
           EAEFW+KSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKP+MKD+EYIE+LK+LI+K
Sbjct: 421 EAEFWKKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPSMKDLEYIEQLKELIQK 480

Query: 481 ENIPAPAPLEQRWTGRSKSPMSPASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHY 540
           E IPAP+P+EQRWTGRSKSPMSPA STAE+DIFSWVGIIMYLPT+D RQRK+IT+EFFHY
Sbjct: 481 EAIPAPSPIEQRWTGRSKSPMSPAFSTAEEDIFSWVGIIMYLPTADPRQRKDITDEFFHY 540

Query: 541 RHLTQTLLWDKFSAFEHWAKIEVPKDKDELAALQARLRKRFPVDEYNKARRALDPNKILS 585
           RHLTQ  LWD++SA+EHWAKIE+PKDK+EL ALQ RLRKRFPVD YNKARR LDPN+ILS
Sbjct: 541 RHLTQAKLWDQYSAYEHWAKIEIPKDKEELEALQERLRKRFPVDAYNKARRELDPNRILS 594

BLAST of Cmc08g0221631 vs. ExPASy Swiss-Prot
Match: Q2RAP0 (L-galactono-1,4-lactone dehydrogenase 1, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=GLDH1 PE=2 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 3.2e-240
Identity = 412/571 (72.15%), Postives = 482/571 (84.41%), Query Frame = 0

Query: 17  RRLQISPTAAISKPPLNSPRPFCTVSPSPSSSFDSELRKYLGYGALVIFCGAATYYSFPF 76
           RR   SP+   S   L+  R     SP P+S  D++LRKY GY  L++ CGAATYYSFP 
Sbjct: 11  RRASSSPS---SHHHLHLVRALSASSPLPAS--DADLRKYAGYALLLLGCGAATYYSFPL 70

Query: 77  PENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPEDLEQLEQVVKQANEKKAR 136
           P +A HKKA  F+YAPLP+DLH VSNWS THEV TRV  QP+ L  L   +  A+ +  +
Sbjct: 71  PPDALHKKAVPFKYAPLPDDLHAVSNWSATHEVHTRVLLQPDSLPALHDALAAAHGECRK 130

Query: 137 VRPVGSGLSPNGIGLSRLGMVNMALMDKVLEVDKEKKRVRVQAGIRVQQLVDGIKEYGLT 196
           +RP+GSGLSPNG+ LSR GMVN+ALMDKVL VD +KK V VQAGIRV +LVD ++E+GLT
Sbjct: 131 LRPLGSGLSPNGLALSRAGMVNLALMDKVLGVDAKKKTVTVQAGIRVAELVDALREHGLT 190

Query: 197 LQNFASIREQQIGGIIQVVGAHGTGAKLPPIDEQVIAMKLVTPAKGTIEISNDKDPELFF 256
           LQNFASIREQQ+GGIIQ VGAHGTGA+LPPIDEQVI+MKLVTPAKGTIE+S +KDP+LF+
Sbjct: 191 LQNFASIREQQVGGIIQ-VGAHGTGARLPPIDEQVISMKLVTPAKGTIELSREKDPDLFY 250

Query: 257 LARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLLADNKHVKYLYIPYTD 316
           LARCGLGGLGVVAEVTLQCVER +L+EHT++SN  ++KKNHKK L++NKH+KYL+IPYTD
Sbjct: 251 LARCGLGGLGVVAEVTLQCVERHQLIEHTFVSNADEVKKNHKKWLSENKHIKYLWIPYTD 310

Query: 317 AIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKY---SASEERDINEFSFT 376
            +VVV CNP S+WR  PKF  KY  +EAIQHVRDLY ESLKKY   + S + ++++ SFT
Sbjct: 311 TVVVVQCNPPSRWR-TPKFTSKYGKDEAIQHVRDLYHESLKKYRTKAESNDPEVDQLSFT 370

Query: 377 ELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPA 436
           ELRD+LL LDPL+K+HV+++N+AEAE+W+KSEGYR+GWSDEILGFDCGGQQWVSETCFPA
Sbjct: 371 ELRDRLLTLDPLDKDHVIRINKAEAEYWKKSEGYRMGWSDEILGFDCGGQQWVSETCFPA 430

Query: 437 GTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTGRSKSPMSPASSTAEDDIFSWVG 496
           GTLAKPNMKD++YIEEL QLIEKE+IPAPAP+EQRWT  S+SPMSPASS+ EDDIFSWVG
Sbjct: 431 GTLAKPNMKDLDYIEELLQLIEKEDIPAPAPIEQRWTACSRSPMSPASSSQEDDIFSWVG 490

Query: 497 IIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDKFSAFEHWAKIEVPKDKDELAALQARL 556
           IIMYLPTSDARQRKEITEEFF+YR  TQT LWD +SA+EHWAKIEVPKDKDELA LQARL
Sbjct: 491 IIMYLPTSDARQRKEITEEFFNYRSKTQTNLWDGYSAYEHWAKIEVPKDKDELAELQARL 550

Query: 557 RKRFPVDEYNKARRALDPNKILSNNKLEKLF 585
           RKRFPVD YNKAR  LDPNK+LSN KLEKLF
Sbjct: 551 RKRFPVDAYNKARMELDPNKVLSNAKLEKLF 574

BLAST of Cmc08g0221631 vs. ExPASy Swiss-Prot
Match: Q2QXY1 (L-galactono-1,4-lactone dehydrogenase 2, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=GLDH2 PE=2 SV=1)

HSP 1 Score: 830.1 bits (2143), Expect = 1.6e-239
Identity = 410/571 (71.80%), Postives = 483/571 (84.59%), Query Frame = 0

Query: 17  RRLQISPTAAISKPPLNSPRPFCTVSPSPSSSFDSELRKYLGYGALVIFCGAATYYSFPF 76
           RR   SP+   S   L+  R     SP P+S  D++LRKY GY  L++ CGAATYYSFP 
Sbjct: 11  RRASSSPS---SHHHLHLVRALSASSPLPAS--DADLRKYAGYALLLLGCGAATYYSFPL 70

Query: 77  PENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPEDLEQLEQVVKQANEKKAR 136
           P +A HKKA  F+YAPLP+DLH VSNWS THEV TRV  QP+ L  L   +  A+ ++ +
Sbjct: 71  PPDALHKKAVPFKYAPLPDDLHAVSNWSATHEVHTRVLLQPDSLPVLHDALAAAHGERRK 130

Query: 137 VRPVGSGLSPNGIGLSRLGMVNMALMDKVLEVDKEKKRVRVQAGIRVQQLVDGIKEYGLT 196
           +RP+GSGLSPNG+ LSR GMVN+ALMDKVL+VD +KK V VQAGIRV +LVD ++E+GLT
Sbjct: 131 LRPLGSGLSPNGLALSRAGMVNLALMDKVLDVDAKKKTVTVQAGIRVAELVDTLREHGLT 190

Query: 197 LQNFASIREQQIGGIIQVVGAHGTGAKLPPIDEQVIAMKLVTPAKGTIEISNDKDPELFF 256
           LQNFASIREQQ+GGIIQ VGAHGTGA+LPPIDEQVI+MKLVTPAKGTIE+S +KDP+LF+
Sbjct: 191 LQNFASIREQQVGGIIQ-VGAHGTGARLPPIDEQVISMKLVTPAKGTIELSREKDPDLFY 250

Query: 257 LARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLLADNKHVKYLYIPYTD 316
           LARCGLGGLGVVAEVTLQCVER +L+EHT++S+  ++KKNHKK L++NKH+KYL+IPYTD
Sbjct: 251 LARCGLGGLGVVAEVTLQCVERHQLIEHTFVSSADEVKKNHKKWLSENKHIKYLWIPYTD 310

Query: 317 AIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKY---SASEERDINEFSFT 376
            +VVV CNP S+WR  PKF  KY  +EAIQHVRDLY ESLKKY   + S + ++++ SFT
Sbjct: 311 TVVVVQCNPPSRWR-TPKFTSKYGKDEAIQHVRDLYRESLKKYRTKAESNDPEVDQLSFT 370

Query: 377 ELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPA 436
           ELRD+LLALDPL+K+HV+++N+AEAE+W+KSEGYR+GWSDEILGFDCGGQQWVSETCFPA
Sbjct: 371 ELRDRLLALDPLDKDHVIRINKAEAEYWKKSEGYRMGWSDEILGFDCGGQQWVSETCFPA 430

Query: 437 GTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTGRSKSPMSPASSTAEDDIFSWVG 496
           GTLAKPNMKD++YIEEL QLIEKE+IPAPAP+EQRWT  S+SPMSPASS+ EDDIFSWVG
Sbjct: 431 GTLAKPNMKDLDYIEELLQLIEKEDIPAPAPIEQRWTACSRSPMSPASSSQEDDIFSWVG 490

Query: 497 IIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDKFSAFEHWAKIEVPKDKDELAALQARL 556
           IIMYLPTSDARQRKEITEEFF+YR  TQT LWD +SA+EHWAKIEVPKDKDEL  L ARL
Sbjct: 491 IIMYLPTSDARQRKEITEEFFNYRSKTQTNLWDGYSAYEHWAKIEVPKDKDELTELLARL 550

Query: 557 RKRFPVDEYNKARRALDPNKILSNNKLEKLF 585
           RKRFPVD YNKAR  LDPNK+LSN KLEKLF
Sbjct: 551 RKRFPVDAYNKARMELDPNKVLSNAKLEKLF 574

BLAST of Cmc08g0221631 vs. ExPASy Swiss-Prot
Match: P10867 (L-gulonolactone oxidase OS=Rattus norvegicus OX=10116 GN=Gulo PE=1 SV=3)

HSP 1 Score: 156.0 bits (393), Expect = 1.3e-36
Identity = 132/485 (27.22%), Postives = 221/485 (45.57%), Query Frame = 0

Query: 102 NWSGTHEVLTRVFHQPEDLEQLEQVVKQANEKKARVRPVGSGLSPNGIGLSRLGMVNMAL 161
           NW+ T+     V++QP  +E++ +V+  A E+K +V+ VG G SP+ I  +   M++M  
Sbjct: 12  NWAKTYGCSPEVYYQPTSVEEVREVLALAREQKKKVKVVGGGHSPSDIACTDGFMIHMGK 71

Query: 162 MDKVLEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVVGAHGTG 221
           M++VL+VDKEKK++ V+AGI +  L   + E+GL + N  ++ +  + G+I   G H TG
Sbjct: 72  MNRVLQVDKEKKQITVEAGILLADLHPQLDEHGLAMSNLGAVSDVTVAGVIG-SGTHNTG 131

Query: 222 AKLPPIDEQVIAMKLVTPAKGTIEISNDKDPELFFLARCGLGGLGVVAEVTLQCVERQEL 281
            K   +  QV+A+ L+T     +E S  ++ ++F  AR  LG LG++  VTLQCV +  L
Sbjct: 132 IKHGILATQVVALTLMTADGEVLECSESRNADVFQAARVHLGCLGIILTVTLQCVPQFHL 191

Query: 282 VEHTYISNMKDIKKNHKKLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTS 341
            E ++ S +K++  N    L  +++ ++L+ P+T+ + ++  +  +K    P     +  
Sbjct: 192 QETSFPSTLKEVLDNLDSHLKRSEYFRFLWFPHTENVSIIYQDHTNK---APSSASNWFW 251

Query: 342 EEAIQHVRDLYVESLKKYSASEERDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFW 401
           + AI      ++     Y       IN F F  L                        F 
Sbjct: 252 DYAIGFYLLEFLLWTSTYLPCLVGWINRFFFWML------------------------FN 311

Query: 402 RKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKE-NIP 461
            K E   +  S +I  ++C  +Q V +        A P  K  E + ELK ++E    + 
Sbjct: 312 CKKESSNL--SHKIFTYECRFKQHVQD-------WAIPREKTKEALLELKAMLEAHPKVV 371

Query: 462 APAPLEQRWTGRSKSPMSPASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLT 521
           A  P+E R+T      +SP           ++ IIMY P      R +          L 
Sbjct: 372 AHYPVEVRFTRGDDILLSPCFQRDS----CYMNIIMYRPYGKDVPRLDY--------WLA 431

Query: 522 QTLLWDKFSAFEHWAKIEVPKDKDELAALQARLRKRFPV-DEYNKARRALDPNKILSNNK 581
              +  KF    HWAK      KD          + +P   ++   R  LDP  +  N+ 
Sbjct: 432 YETIMKKFGGRPHWAKAHNCTQKD--------FEEMYPTFHKFCDIREKLDPTGMFLNSY 439

Query: 582 LEKLF 585
           LEK+F
Sbjct: 492 LEKVF 439

BLAST of Cmc08g0221631 vs. ExPASy TrEMBL
Match: Q9M4U9 (L-galactono-1,4-lactone dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103497260 PE=2 SV=2)

HSP 1 Score: 1176.4 bits (3042), Expect = 0.0e+00
Identity = 587/590 (99.49%), Postives = 588/590 (99.66%), Query Frame = 0

Query: 1   MLNFLSLRRSLHYFRHRRLQISPTAAISKPPLNSPRPFCTVSPSPSSSFDSELRKYLGYG 60
           MLNFLSLRRSLHYFRHRRLQISPTAAISKPPLNSPRPFCTVSPSPSSSFDSELRKYLGYG
Sbjct: 1   MLNFLSLRRSLHYFRHRRLQISPTAAISKPPLNSPRPFCTVSPSPSSSFDSELRKYLGYG 60

Query: 61  ALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPEDL 120
           ALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPEDL
Sbjct: 61  ALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPEDL 120

Query: 121 EQLEQVVKQANEKKARVRPVGSGLSPNGIGLSRLGMVNMALMDKVLEVDKEKKRVRVQAG 180
           EQLEQVVKQANEKKARVRPVGSGLSPNGIGLSRLGMVNMALMDKVLEVDKEKKRVRVQAG
Sbjct: 121 EQLEQVVKQANEKKARVRPVGSGLSPNGIGLSRLGMVNMALMDKVLEVDKEKKRVRVQAG 180

Query: 181 IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVVGAHGTGAKLPPIDEQVIAMKLVTPA 240
           IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQ VGAHGTGAKLPPIDEQVIAMKLVTPA
Sbjct: 181 IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQ-VGAHGTGAKLPPIDEQVIAMKLVTPA 240

Query: 241 KGTIEISNDKDPELFFLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL 300
           KGTIEISNDKDPELFFLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL
Sbjct: 241 KGTIEISNDKDPELFFLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL 300

Query: 301 LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYS 360
           LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYS
Sbjct: 301 LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYS 360

Query: 361 ASEERDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDC 420
           ASEERDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGY+VGWSDEILGFDC
Sbjct: 361 ASEERDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYKVGWSDEILGFDC 420

Query: 421 GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTGRSKSPMSPA 480
           GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTGRSKSPMSPA
Sbjct: 421 GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTGRSKSPMSPA 480

Query: 481 SSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDKFSAFEHWAKIEVP 540
           SSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDKFSAFEHWAKIEVP
Sbjct: 481 SSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDKFSAFEHWAKIEVP 540

Query: 541 KDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKLFSSLDMV 591
           KDKDELAALQARLRKRFPVDE NKARRALDPNKILSNNKLEKLFSSLDMV
Sbjct: 541 KDKDELAALQARLRKRFPVDESNKARRALDPNKILSNNKLEKLFSSLDMV 589

BLAST of Cmc08g0221631 vs. ExPASy TrEMBL
Match: E7E016 (L-galactono-1,4-lactone dehydrogenase OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 1141.3 bits (2951), Expect = 0.0e+00
Identity = 569/591 (96.28%), Postives = 579/591 (97.97%), Query Frame = 0

Query: 1   MLNFLSLRRSLHYFRHRRLQISPTAAISKPPLNSPRPFCTVSPSP-SSSFDSELRKYLGY 60
           MLNFLSLRRS+HYFRHRRLQISPTAAISKPP N PRPF TVSPSP SSSFDSELRKYLGY
Sbjct: 1   MLNFLSLRRSIHYFRHRRLQISPTAAISKPPFNPPRPFSTVSPSPSSSSFDSELRKYLGY 60

Query: 61  GALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPED 120
           GALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEV TRVFHQPE+
Sbjct: 61  GALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVWTRVFHQPEN 120

Query: 121 LEQLEQVVKQANEKKARVRPVGSGLSPNGIGLSRLGMVNMALMDKVLEVDKEKKRVRVQA 180
           LEQLEQVVKQANEKKAR+RPVGSGLSPNGIGLSR+GMVN+ALMDKVLEVDKEKKRVRVQA
Sbjct: 121 LEQLEQVVKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQA 180

Query: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVVGAHGTGAKLPPIDEQVIAMKLVTP 240
           GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQ VGAHGTGAKLPPIDEQVIAMKLVTP
Sbjct: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQ-VGAHGTGAKLPPIDEQVIAMKLVTP 240

Query: 241 AKGTIEISNDKDPELFFLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKK 300
           AKGTIEIS DKDPELF LARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKK
Sbjct: 241 AKGTIEISKDKDPELFLLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKK 300

Query: 301 LLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKY 360
           LLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKY
Sbjct: 301 LLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKY 360

Query: 361 SASEERDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFD 420
           SASEERD+NE SFTELRDKLLALDPLNKEHV+KVNQAEAEFWRKSEGYRVGWSDEILGFD
Sbjct: 361 SASEERDMNEISFTELRDKLLALDPLNKEHVIKVNQAEAEFWRKSEGYRVGWSDEILGFD 420

Query: 421 CGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTGRSKSPMSP 480
           CGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEK+NIPAPAPLEQRWT RSKSPMSP
Sbjct: 421 CGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKKNIPAPAPLEQRWTARSKSPMSP 480

Query: 481 ASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDKFSAFEHWAKIEV 540
           ASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWD++SAFEHWAKIEV
Sbjct: 481 ASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEV 540

Query: 541 PKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKLFSSLDMV 591
           PKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKLFSS D V
Sbjct: 541 PKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKLFSSTDTV 590

BLAST of Cmc08g0221631 vs. ExPASy TrEMBL
Match: A0A6J1EEF4 (L-galactono-1,4-lactone dehydrogenase, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111432481 PE=4 SV=1)

HSP 1 Score: 1084.3 bits (2803), Expect = 0.0e+00
Identity = 541/591 (91.54%), Postives = 565/591 (95.60%), Query Frame = 0

Query: 1   MLNFLSLRRSLHYFRHRRLQISPTAAISKPPLNSPRPFCTVS-PSPSSSFDSELRKYLGY 60
           ML  LSLRRSLH   HRR    PT AIS+PPLN  R F TVS P PSSS +SELRKY+GY
Sbjct: 1   MLRLLSLRRSLH-LHHRRPHFPPTVAISRPPLNPTRQFSTVSPPPPSSSSESELRKYIGY 60

Query: 61  GALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPED 120
           GALVI CGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPE+
Sbjct: 61  GALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPEN 120

Query: 121 LEQLEQVVKQANEKKARVRPVGSGLSPNGIGLSRLGMVNMALMDKVLEVDKEKKRVRVQA 180
           LEQLE+VVK++N KKAR+RPVGSGLSPNGIGLSR+GMVN+ALMD+VLEVDKEKKRVRVQA
Sbjct: 121 LEQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDEVLEVDKEKKRVRVQA 180

Query: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVVGAHGTGAKLPPIDEQVIAMKLVTP 240
           GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQ VGAHGTGAKLPPIDEQVIAMKLVTP
Sbjct: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQ-VGAHGTGAKLPPIDEQVIAMKLVTP 240

Query: 241 AKGTIEISNDKDPELFFLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKK 300
           AKGTIEIS +KDPELF+LARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKK
Sbjct: 241 AKGTIEISREKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKK 300

Query: 301 LLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKY 360
           LLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKF+PKYTS+EAIQHVRDLYVESLKKY
Sbjct: 301 LLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLKKY 360

Query: 361 SASEERDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFD 420
           SAS+ERDINEFSFTELRDKLLALDPLNKEHV+KVNQAEAEFWRKSEGYRVGWSDEILGFD
Sbjct: 361 SASDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWRKSEGYRVGWSDEILGFD 420

Query: 421 CGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTGRSKSPMSP 480
           CGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAP+EQRWT  SKSPMSP
Sbjct: 421 CGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSP 480

Query: 481 ASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDKFSAFEHWAKIEV 540
           ASS+AEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWD++SAFEHWAKIEV
Sbjct: 481 ASSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEV 540

Query: 541 PKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKLFSSLDMV 591
           PKDKDELAALQARLRKRFPVDEYNKARR LDPNKILS+NKLEK+F S D+V
Sbjct: 541 PKDKDELAALQARLRKRFPVDEYNKARRELDPNKILSSNKLEKMFVSSDVV 589

BLAST of Cmc08g0221631 vs. ExPASy TrEMBL
Match: A0A6J1HRG8 (L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111465966 PE=4 SV=1)

HSP 1 Score: 1081.6 bits (2796), Expect = 0.0e+00
Identity = 541/591 (91.54%), Postives = 564/591 (95.43%), Query Frame = 0

Query: 1   MLNFLSLRRSLHYFRHRRLQISPTAAISKPPLNSPRPFCTVS-PSPSSSFDSELRKYLGY 60
           ML  LSLRRSLH   HRR Q  PT AISKPPLN  R F TVS P PSSS +SELRKYLGY
Sbjct: 1   MLKLLSLRRSLH-LHHRRPQFPPTVAISKPPLNPTRQFSTVSPPPPSSSSESELRKYLGY 60

Query: 61  GALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPED 120
           GALVIFCGA TYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEV TRVFHQP++
Sbjct: 61  GALVIFCGATTYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVHTRVFHQPDN 120

Query: 121 LEQLEQVVKQANEKKARVRPVGSGLSPNGIGLSRLGMVNMALMDKVLEVDKEKKRVRVQA 180
           LEQL++VVK+AN KKAR+RPVGSGLSPNGIGLSR+GMVN+ALMDKVLEVDKEKKRVRVQA
Sbjct: 121 LEQLQEVVKEANVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQA 180

Query: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVVGAHGTGAKLPPIDEQVIAMKLVTP 240
           GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQ VGAHGTGAKLPPIDEQVIAMKLVTP
Sbjct: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQ-VGAHGTGAKLPPIDEQVIAMKLVTP 240

Query: 241 AKGTIEISNDKDPELFFLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKK 300
           AKGTIEIS +KDPELF+LARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKK
Sbjct: 241 AKGTIEISKEKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKK 300

Query: 301 LLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKY 360
           LLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKF+PKYTS+EAIQHVRDLYVESLKKY
Sbjct: 301 LLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLKKY 360

Query: 361 SASEERDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFD 420
           SAS+ERDINEFSFTELRDKLLALDPLNKEHV+KVNQAEAEFW KSEGYRVGWSDEILGFD
Sbjct: 361 SASDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWWKSEGYRVGWSDEILGFD 420

Query: 421 CGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTGRSKSPMSP 480
           CGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAP+EQRWT  SKSPMSP
Sbjct: 421 CGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSP 480

Query: 481 ASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDKFSAFEHWAKIEV 540
           ASS++EDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWD++SAFEHWAKIEV
Sbjct: 481 ASSSSEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEV 540

Query: 541 PKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKLFSSLDMV 591
           PKDKDELAALQARLRKRFPVDEYNKAR+ LDPNKILS+NKLEKLF S D+V
Sbjct: 541 PKDKDELAALQARLRKRFPVDEYNKARQELDPNKILSSNKLEKLFVSSDVV 589

BLAST of Cmc08g0221631 vs. ExPASy TrEMBL
Match: A0A6J1DK81 (L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111021307 PE=4 SV=1)

HSP 1 Score: 1044.3 bits (2699), Expect = 2.0e-301
Identity = 523/594 (88.05%), Postives = 552/594 (92.93%), Query Frame = 0

Query: 1   MLNFLSLRRSLHYFRHRRLQISPTAAISKPPLN---SPRPFCTVS-PSPSSSFDSELRKY 60
           ML  L LRRS     HRRL   P AAIS+ PLN    P  F TVS P PSSSFDSELRKY
Sbjct: 1   MLRILPLRRS-----HRRLLSPPAAAISRTPLNHHHHPPSFSTVSPPPPSSSFDSELRKY 60

Query: 61  LGYGALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQ 120
           LGYGALV+FCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEV TRVF Q
Sbjct: 61  LGYGALVVFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVQTRVFQQ 120

Query: 121 PEDLEQLEQVVKQANEKKARVRPVGSGLSPNGIGLSRLGMVNMALMDKVLEVDKEKKRVR 180
           PE+LEQLEQVVK+ANEKKAR+RPVGSGLSPNGIGLSR+GMVN+ALMDKVLEVDKEKKRVR
Sbjct: 121 PENLEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVR 180

Query: 181 VQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVVGAHGTGAKLPPIDEQVIAMKL 240
           VQAGIRVQQLVDGIKE+GLTLQNFASIREQQIGGIIQ VGAHGTG KLPPIDEQVIAMKL
Sbjct: 181 VQAGIRVQQLVDGIKEHGLTLQNFASIREQQIGGIIQ-VGAHGTGPKLPPIDEQVIAMKL 240

Query: 241 VTPAKGTIEISNDKDPELFFLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKN 300
           VTPAKGTIEIS +KDPELF+LARCGLGGLGVVAEVTLQCVERQELVEHTYISNM DIKKN
Sbjct: 241 VTPAKGTIEISKEKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMNDIKKN 300

Query: 301 HKKLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESL 360
           HKKLL DNKHVKYLYIPYTDA+VVVTCNPISKWRGPPK KPKYTS+EAIQHVRDLY ESL
Sbjct: 301 HKKLLTDNKHVKYLYIPYTDAVVVVTCNPISKWRGPPKLKPKYTSDEAIQHVRDLYAESL 360

Query: 361 KKYSASEERDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEIL 420
           KKYS S+E DINE+S+TELRDKLLALDPLNKEHV KVNQAEAEFWRKSEGY+VGWSDEIL
Sbjct: 361 KKYSTSDETDINEYSYTELRDKLLALDPLNKEHVTKVNQAEAEFWRKSEGYKVGWSDEIL 420

Query: 421 GFDCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTGRSKSP 480
           GFDCGGQQWVSETCFPAGTLAKP+MKDI+YIE+LKQLIEKENIPAPAP+EQRWT  SKSP
Sbjct: 421 GFDCGGQQWVSETCFPAGTLAKPSMKDIKYIEDLKQLIEKENIPAPAPIEQRWTACSKSP 480

Query: 481 MSPASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDKFSAFEHWAK 540
           MSPASS A+DD+FSWVGIIMYLPT DARQRKEITEEFFHYRHLTQ+ LWD++SAFEHWAK
Sbjct: 481 MSPASSLAQDDLFSWVGIIMYLPTLDARQRKEITEEFFHYRHLTQSQLWDQYSAFEHWAK 540

Query: 541 IEVPKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKLFSSLDMV 591
           IEVP+DK+ELAALQARLRKRFPVDE+NKA+RALDPNKILSNNKLEKLF S D V
Sbjct: 541 IEVPRDKEELAALQARLRKRFPVDEFNKAQRALDPNKILSNNKLEKLFPSNDAV 588

BLAST of Cmc08g0221631 vs. TAIR 10
Match: AT3G47930.1 (L-galactono-1,4-lactone dehydrogenase )

HSP 1 Score: 918.3 bits (2372), Expect = 3.1e-267
Identity = 459/608 (75.49%), Postives = 513/608 (84.38%), Query Frame = 0

Query: 1   MLNFLSLRRSLHY---------------FRHRRLQISPTAAISKPPLNSPRPFCTVSPSP 60
           ML  L LRRS+ +               F   R   +    ++ PP   PRP     P P
Sbjct: 1   MLRSLLLRRSVGHSLGTLSPSSSTIRSSFSPHRTLCTTGQTLTPPPPPPPRP---PPPPP 60

Query: 61  SSSFDSELRKYLGYGALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSG 120
           +++ +++ RKY GY AL IF G ATY+SFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSG
Sbjct: 61  ATASEAQFRKYAGYAALAIFSGVATYFSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSG 120

Query: 121 THEVLTRVFHQPEDLEQLEQVVKQANEKKARVRPVGSGLSPNGIGLSRLGMVNMALMDKV 180
           THEV TR F+QPE+L  LE +VK+++EKK R+RPVGSGLSPNGIGLSR GMVN+ALMDKV
Sbjct: 121 THEVQTRNFNQPENLADLEALVKESHEKKLRIRPVGSGLSPNGIGLSRSGMVNLALMDKV 180

Query: 181 LEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVVGAHGTGAKLP 240
           LEVDKEKKRV VQAGIRVQQLVD IK+YGLTLQNFASIREQQIGGIIQ VGAHGTGA+LP
Sbjct: 181 LEVDKEKKRVTVQAGIRVQQLVDAIKDYGLTLQNFASIREQQIGGIIQ-VGAHGTGARLP 240

Query: 241 PIDEQVIAMKLVTPAKGTIEISNDKDPELFFLARCGLGGLGVVAEVTLQCVERQELVEHT 300
           PIDEQVI+MKLVTPAKGTIE+S +KDPELF LARCGLGGLGVVAEVTLQCV R ELVEHT
Sbjct: 241 PIDEQVISMKLVTPAKGTIELSREKDPELFHLARCGLGGLGVVAEVTLQCVARHELVEHT 300

Query: 301 YISNMKDIKKNHKKLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAI 360
           Y+SN+++IKKNHKKLL+ NKHVKYLYIPYTD +VVVTCNP+SKW GPPK KPKYT++EA+
Sbjct: 301 YVSNLQEIKKNHKKLLSANKHVKYLYIPYTDTVVVVTCNPVSKWSGPPKDKPKYTTDEAV 360

Query: 361 QHVRDLYVESLKKYSA---------SEERDINEFSFTELRDKLLALDPLNKEHVMKVNQA 420
           QHVRDLY ES+ KY           S E DI E SFTELRDKLLALDPLN  HV KVNQA
Sbjct: 361 QHVRDLYRESIVKYRVQDSGKKSPDSSEPDIQELSFTELRDKLLALDPLNDVHVAKVNQA 420

Query: 421 EAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEK 480
           EAEFW+KSEGYRVGWSDEILGFDCGGQQWVSE+CFPAGTLA P+MKD+EYIEELK+LIEK
Sbjct: 421 EAEFWKKSEGYRVGWSDEILGFDCGGQQWVSESCFPAGTLANPSMKDLEYIEELKKLIEK 480

Query: 481 ENIPAPAPLEQRWTGRSKSPMSPASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHY 540
           E IPAPAP+EQRWT RSKSP+SPA ST+EDDIFSWVGIIMYLPT+D RQRK+IT+EFFHY
Sbjct: 481 EAIPAPAPIEQRWTARSKSPISPAFSTSEDDIFSWVGIIMYLPTADPRQRKDITDEFFHY 540

Query: 541 RHLTQTLLWDKFSAFEHWAKIEVPKDKDELAALQARLRKRFPVDEYNKARRALDPNKILS 585
           RHLTQ  LWD+FSA+EHWAKIE+PKDK+EL ALQAR+RKRFPVD YNKARR LDPN+ILS
Sbjct: 541 RHLTQKQLWDQFSAYEHWAKIEIPKDKEELEALQARIRKRFPVDAYNKARRELDPNRILS 600

BLAST of Cmc08g0221631 vs. TAIR 10
Match: AT3G47930.2 (L-galactono-1,4-lactone dehydrogenase )

HSP 1 Score: 727.2 bits (1876), Expect = 1.0e-209
Identity = 368/497 (74.04%), Postives = 410/497 (82.49%), Query Frame = 0

Query: 1   MLNFLSLRRSLHY---------------FRHRRLQISPTAAISKPPLNSPRPFCTVSPSP 60
           ML  L LRRS+ +               F   R   +    ++ PP   PRP     P P
Sbjct: 1   MLRSLLLRRSVGHSLGTLSPSSSTIRSSFSPHRTLCTTGQTLTPPPPPPPRP---PPPPP 60

Query: 61  SSSFDSELRKYLGYGALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSG 120
           +++ +++ RKY GY AL IF G ATY+SFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSG
Sbjct: 61  ATASEAQFRKYAGYAALAIFSGVATYFSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSG 120

Query: 121 THEVLTRVFHQPEDLEQLEQVVKQANEKKARVRPVGSGLSPNGIGLSRLGMVNMALMDKV 180
           THEV TR F+QPE+L  LE +VK+++EKK R+RPVGSGLSPNGIGLSR GMVN+ALMDKV
Sbjct: 121 THEVQTRNFNQPENLADLEALVKESHEKKLRIRPVGSGLSPNGIGLSRSGMVNLALMDKV 180

Query: 181 LEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVVGAHGTGAKLP 240
           LEVDKEKKRV VQAGIRVQQLVD IK+YGLTLQNFASIREQQIGGIIQ VGAHGTGA+LP
Sbjct: 181 LEVDKEKKRVTVQAGIRVQQLVDAIKDYGLTLQNFASIREQQIGGIIQ-VGAHGTGARLP 240

Query: 241 PIDEQVIAMKLVTPAKGTIEISNDKDPELFFLARCGLGGLGVVAEVTLQCVERQELVEHT 300
           PIDEQVI+MKLVTPAKGTIE+S +KDPELF LARCGLGGLGVVAEVTLQCV R ELVEHT
Sbjct: 241 PIDEQVISMKLVTPAKGTIELSREKDPELFHLARCGLGGLGVVAEVTLQCVARHELVEHT 300

Query: 301 YISNMKDIKKNHKKLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAI 360
           Y+SN+++IKKNHKKLL+ NKHVKYLYIPYTD +VVVTCNP+SKW GPPK KPKYT++EA+
Sbjct: 301 YVSNLQEIKKNHKKLLSANKHVKYLYIPYTDTVVVVTCNPVSKWSGPPKDKPKYTTDEAV 360

Query: 361 QHVRDLYVESLKKYSA---------SEERDINEFSFTELRDKLLALDPLNKEHVMKVNQA 420
           QHVRDLY ES+ KY           S E DI E SFTELRDKLLALDPLN  HV KVNQA
Sbjct: 361 QHVRDLYRESIVKYRVQDSGKKSPDSSEPDIQELSFTELRDKLLALDPLNDVHVAKVNQA 420

Query: 421 EAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEK 474
           EAEFW+KSEGYRVGWSDEILGFDCGGQQWVSE+CFPAGTLA P+MKD+EYIEELK+LIEK
Sbjct: 421 EAEFWKKSEGYRVGWSDEILGFDCGGQQWVSESCFPAGTLANPSMKDLEYIEELKKLIEK 480

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
NP_001284400.10.0e+0099.49L-galactono-1,4-lactone dehydrogenase, mitochondrial [Cucumis melo] >AAF64319.2 ... [more]
NP_001292704.10.0e+0096.28L-galactono-1,4-lactone dehydrogenase, mitochondrial [Cucumis sativus] >ADT80722... [more]
KAE8637410.10.0e+0096.40hypothetical protein CSA_023387 [Cucumis sativus][more]
XP_038882838.10.0e+0092.55L-galactono-1,4-lactone dehydrogenase, mitochondrial isoform X1 [Benincasa hispi... [more]
XP_023517263.10.0e+0092.22L-galactono-1,4-lactone dehydrogenase, mitochondrial [Cucurbita pepo subsp. pepo... [more]
Match NameE-valueIdentityDescription
Q9SU564.4e-26675.49L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Arabidopsis thaliana OX=... [more]
O478817.5e-26675.99L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Brassica oleracea OX=371... [more]
Q2RAP03.2e-24072.15L-galactono-1,4-lactone dehydrogenase 1, mitochondrial OS=Oryza sativa subsp. ja... [more]
Q2QXY11.6e-23971.80L-galactono-1,4-lactone dehydrogenase 2, mitochondrial OS=Oryza sativa subsp. ja... [more]
P108671.3e-3627.22L-gulonolactone oxidase OS=Rattus norvegicus OX=10116 GN=Gulo PE=1 SV=3[more]
Match NameE-valueIdentityDescription
Q9M4U90.0e+0099.49L-galactono-1,4-lactone dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103497260 PE... [more]
E7E0160.0e+0096.28L-galactono-1,4-lactone dehydrogenase OS=Cucumis sativus OX=3659 PE=2 SV=1[more]
A0A6J1EEF40.0e+0091.54L-galactono-1,4-lactone dehydrogenase, mitochondrial-like OS=Cucurbita moschata ... [more]
A0A6J1HRG80.0e+0091.54L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Cucurbita maxima OX=3661... [more]
A0A6J1DK812.0e-30188.05L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Momordica charantia OX=3... [more]
Match NameE-valueIdentityDescription
AT3G47930.13.1e-26775.49L-galactono-1,4-lactone dehydrogenase [more]
AT3G47930.21.0e-20974.04L-galactono-1,4-lactone dehydrogenase [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010031L-gulonolactone/D-arabinono-1,4-lactone oxidasePIRSFPIRSF000136LGO_GLOcoord: 95..589
e-value: 7.7E-58
score: 194.2
IPR010031L-gulonolactone/D-arabinono-1,4-lactone oxidasePANTHERPTHR43762L-GULONOLACTONE OXIDASEcoord: 93..584
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 113..244
e-value: 9.5E-24
score: 83.7
IPR010029Galactonolactone dehydrogenaseTIGRFAMTIGR01676TIGR01676coord: 50..585
e-value: 0.0
score: 1221.9
IPR016167FAD-binding, type PCMH, subdomain 1GENE3D3.30.43.10coord: 95..163
e-value: 4.3E-12
score: 47.2
IPR007173D-arabinono-1,4-lactone oxidasePFAMPF04030ALOcoord: 271..581
e-value: 9.7E-16
score: 58.3
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 165..293
e-value: 1.2E-26
score: 94.6
NoneNo IPR availablePANTHERPTHR43762:SF1L-GULONOLACTONE OXIDASEcoord: 93..584
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 106..278
score: 17.412628
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 94..276

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc08g0221631.1Cmc08g0221631.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019853 L-ascorbic acid biosynthetic process
cellular_component GO:0016020 membrane
molecular_function GO:0003885 D-arabinono-1,4-lactone oxidase activity
molecular_function GO:0071949 FAD binding
molecular_function GO:0016633 galactonolactone dehydrogenase activity
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0016899 oxidoreductase activity, acting on the CH-OH group of donors, oxygen as acceptor