Cmc08g0221561 (gene) Melon (Charmono) v1.1

Overview


Name	Cmc08g0221561
Type	gene
Organism	Cucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Description	Reverse transcriptase
Location	CMiso1.1chr08: 9371436 .. 9372239 (-)
RNA-Seq Expression	Cmc08g0221561
Synteny	Cmc08g0221561

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCCCAGCAGAGTTGAAAGAGCTGAAGGTGCAGTTACAAGAGTTACTTGATAAAGGCTTCATTCGACCGAGTGTGTCACCTTCGGGTGTACCAGTCTTATTTGTTAAGAAGAAGGATGGATCGATGCGCCTATGTATTGACTACAGGGAGTTGAATAAGGTCACCGTTCAGAACAAATATCCCTTGCCCAGGATCGACGATCTATTTGACCAGTTACAAGGAGCTACAGTGTTCTCTAAGATCGACCTTCGATCGGGATATCATCAGCTGAGGATTAAGGATAGCGATGTACCGAAGATAACCTTTCGTTCCAGATATGGACACTATGAGTTTATTATAATGTCTTTTGGTTTGACGAATGCTCCGATAGTGTTTATGGATTTGATGAACAGAGTGTTTAGGGAGCTCCTAGACACTTTTGTGATCGTGTTTATTGATGATATTTTGATATATTCCAAGACAGAGGCCGAGCATAAGGAGCATTTACGTATGGTTTTAGAAACCCTTCGAGCTAATGAACTGTTTGCAAAGTTCTCAAAATGTGAGTTTTGGTTGAAGCAGGTATCCTTTCTAGGCCATGTGGTTTCTAAAGCTGGTATTTCGATGGATCCAGCTAAGATAGAGGCAGTCACCAGTTGGCCTCGACCTTCCACAGTCAGTGAGGTTCATAGTTTTCTTAGTTTAGCGGGTTATTATCGACGGTTTATTGAGAACTTTTCCCGTATAGCTACTCCTCTTACTCAGTTGACCAGGAAGAGAGCTTCTTTAGTGTGGAGCAAGGCCTGTAAGGACAATTTTTAG

mRNA sequence

ATGGCCCCAGCAGAGTTGAAAGAGCTGAAGGTGCAGTTACAAGAGTTACTTGATAAAGGCTTCATTCGACCGAGTGTGTCACCTTCGGGTGTACCAGTCTTATTTGTTAAGAAGAAGGATGGATCGATGCGCCTATGTATTGACTACAGGGAGTTGAATAAGGTCACCGTTCAGAACAAATATCCCTTGCCCAGGATCGACGATCTATTTGACCAGTTACAAGGAGCTACAGTGTTCTCTAAGATCGACCTTCGATCGGGATATCATCAGCTGAGGATTAAGGATAGCGATGTACCGAAGATAACCTTTCGTTCCAGATATGGACACTATGAGTTTATTATAATGTCTTTTGGTTTGACGAATGCTCCGATAGTGTTTATGGATTTGATGAACAGAGTGTTTAGGGAGCTCCTAGACACTTTTGTGATCGTGTTTATTGATGATATTTTGATATATTCCAAGACAGAGGCCGAGCATAAGGAGCATTTACGTATGGTTTTAGAAACCCTTCGAGCTAATGAACTGTTTGCAAAGTTCTCAAAATGTGAGTTTTGGTTGAAGCAGGTATCCTTTCTAGGCCATGTGGTTTCTAAAGCTGGTATTTCGATGGATCCAGCTAAGATAGAGGCAGTCACCAGTTGGCCTCGACCTTCCACAGTCAGTGAGGTTCATAGTTTTCTTAGTTTAGCGGGTTATTATCGACGGTTTATTGAGAACTTTTCCCGTATAGCTACTCCTCTTACTCAGTTGACCAGGAAGAGAGCTTCTTTAGTGTGGAGCAAGGCCTGTAAGGACAATTTTTAG

Coding sequence (CDS)

ATGGCCCCAGCAGAGTTGAAAGAGCTGAAGGTGCAGTTACAAGAGTTACTTGATAAAGGCTTCATTCGACCGAGTGTGTCACCTTCGGGTGTACCAGTCTTATTTGTTAAGAAGAAGGATGGATCGATGCGCCTATGTATTGACTACAGGGAGTTGAATAAGGTCACCGTTCAGAACAAATATCCCTTGCCCAGGATCGACGATCTATTTGACCAGTTACAAGGAGCTACAGTGTTCTCTAAGATCGACCTTCGATCGGGATATCATCAGCTGAGGATTAAGGATAGCGATGTACCGAAGATAACCTTTCGTTCCAGATATGGACACTATGAGTTTATTATAATGTCTTTTGGTTTGACGAATGCTCCGATAGTGTTTATGGATTTGATGAACAGAGTGTTTAGGGAGCTCCTAGACACTTTTGTGATCGTGTTTATTGATGATATTTTGATATATTCCAAGACAGAGGCCGAGCATAAGGAGCATTTACGTATGGTTTTAGAAACCCTTCGAGCTAATGAACTGTTTGCAAAGTTCTCAAAATGTGAGTTTTGGTTGAAGCAGGTATCCTTTCTAGGCCATGTGGTTTCTAAAGCTGGTATTTCGATGGATCCAGCTAAGATAGAGGCAGTCACCAGTTGGCCTCGACCTTCCACAGTCAGTGAGGTTCATAGTTTTCTTAGTTTAGCGGGTTATTATCGACGGTTTATTGAGAACTTTTCCCGTATAGCTACTCCTCTTACTCAGTTGACCAGGAAGAGAGCTTCTTTAGTGTGGAGCAAGGCCTGTAAGGACAATTTTTAG

Protein sequence

MAPAELKELKVQLQELLDKGFIRPSVSPSGVPVLFVKKKDGSMRLCIDYRELNKVTVQNKYPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKDSDVPKITFRSRYGHYEFIIMSFGLTNAPIVFMDLMNRVFRELLDTFVIVFIDDILIYSKTEAEHKEHLRMVLETLRANELFAKFSKCEFWLKQVSFLGHVVSKAGISMDPAKIEAVTSWPRPSTVSEVHSFLSLAGYYRRFIENFSRIATPLTQLTRKRASLVWSKACKDNF

Homology

[Close]

BLAST of Cmc08g0221561 vs. NCBI nr
Match: KAA0052348.1 (putative gag-pol polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 490.3 bits (1261), Expect = 1.0e-134
Identity = 239/267 (89.51%), Postives = 254/267 (95.13%), Query Frame = 0


Query: 1   MAPAELKELKVQLQELLDKGFIRPSVSPSGVPVLFVKKKDGSMRLCIDYRELNKVTVQNK 60
           MAPAELKELKVQLQELLDKGFIR SVSP G PVLFVKKKDGSMRLCIDYRELNKVTV+N+
Sbjct: 456 MAPAELKELKVQLQELLDKGFIRSSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKNR 515

Query: 61  YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKDSDVPKITFRSRYGHYEFIIMSFGLT 120
           YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKD DVPKI FRSRYGHYEFI+MSFGLT
Sbjct: 516 YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKDGDVPKIAFRSRYGHYEFIVMSFGLT 575

Query: 121 NAPIVFMDLMNRVFRELLDTFVIVFIDDILIYSKTEAEHKEHLRMVLETLRANELFAKFS 180
           NAP VFMDLMNRVFRE LDTFVIVFIDDILIYSKTEAEH+EHLRMVL+TLR N+L+AKFS
Sbjct: 576 NAPTVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLQTLRDNKLYAKFS 635

Query: 181 KCEFWLKQVSFLGHVVSKAGISMDPAKIEAVTSWPRPSTVSEVHSFLSLAGYYRRFIENF 240
           KCEFWLKQVSFLGHVVSKAG+ +DPAKIEA+TSWPRP TVS+VHSFL LAGYYR+F+ENF
Sbjct: 636 KCEFWLKQVSFLGHVVSKAGVYVDPAKIEAITSWPRPFTVSKVHSFLGLAGYYRQFVENF 695

Query: 241 SRIATPLTQLTRKRASLVWSKACKDNF 268
           SRIATPLTQLTRKRA  VWSKAC+D+F
Sbjct: 696 SRIATPLTQLTRKRAPFVWSKACEDSF 722

[Close]

BLAST of Cmc08g0221561 vs. NCBI nr
Match: TYK01903.1 (putative gag-pol polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 490.3 bits (1261), Expect = 1.0e-134
Identity = 239/267 (89.51%), Postives = 254/267 (95.13%), Query Frame = 0


Query: 1   MAPAELKELKVQLQELLDKGFIRPSVSPSGVPVLFVKKKDGSMRLCIDYRELNKVTVQNK 60
           MAPAELKELKVQLQELLDKGFIR SVSP G PVLFVKKKDGSMRLCIDYRELNKVTV+N+
Sbjct: 456 MAPAELKELKVQLQELLDKGFIRSSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKNR 515

Query: 61  YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKDSDVPKITFRSRYGHYEFIIMSFGLT 120
           YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKD DVPKI FRSRYGHYEFI+MSFGLT
Sbjct: 516 YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKDGDVPKIAFRSRYGHYEFIVMSFGLT 575

Query: 121 NAPIVFMDLMNRVFRELLDTFVIVFIDDILIYSKTEAEHKEHLRMVLETLRANELFAKFS 180
           NAP VFMDLMNRVFRE LDTFVIVFIDDILIYSKTEAEH+EHLRMVL+TLR N+L+AKFS
Sbjct: 576 NAPTVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLQTLRDNKLYAKFS 635

Query: 181 KCEFWLKQVSFLGHVVSKAGISMDPAKIEAVTSWPRPSTVSEVHSFLSLAGYYRRFIENF 240
           KCEFWLKQVSFLGHVVSKAG+ +DPAKIEA+TSWPRP TVS+VHSFL LAGYYR+F+ENF
Sbjct: 636 KCEFWLKQVSFLGHVVSKAGVYVDPAKIEAITSWPRPFTVSKVHSFLGLAGYYRQFVENF 695

Query: 241 SRIATPLTQLTRKRASLVWSKACKDNF 268
           SRIATPLTQLTRKRA  VWSKAC+D+F
Sbjct: 696 SRIATPLTQLTRKRAPFVWSKACEDSF 722

[Close]

BLAST of Cmc08g0221561 vs. NCBI nr
Match: KAA0038231.1 (gag protease polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 489.6 bits (1259), Expect = 1.7e-134
Identity = 240/267 (89.89%), Postives = 252/267 (94.38%), Query Frame = 0


Query: 1   MAPAELKELKVQLQELLDKGFIRPSVSPSGVPVLFVKKKDGSMRLCIDYRELNKVTVQNK 60
           MAPAELKELKVQLQELLDKGFIRPSVSP G PVLFVKKKDGSMRLCIDYRELNKVTV+N+
Sbjct: 554 MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKNR 613

Query: 61  YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKDSDVPKITFRSRYGHYEFIIMSFGLT 120
           YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKD DVPK  FRSRYGHYEFI+MSFGLT
Sbjct: 614 YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKDEDVPKTAFRSRYGHYEFIVMSFGLT 673

Query: 121 NAPIVFMDLMNRVFRELLDTFVIVFIDDILIYSKTEAEHKEHLRMVLETLRANELFAKFS 180
           NAP VFMDLMNRVFRE LDTFVIVFIDDILIYSKTEAEHKEHLRMVL+TLR N+L++KFS
Sbjct: 674 NAPAVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHKEHLRMVLQTLRDNKLYSKFS 733

Query: 181 KCEFWLKQVSFLGHVVSKAGISMDPAKIEAVTSWPRPSTVSEVHSFLSLAGYYRRFIENF 240
           KCEFWLKQVSFLGHVVSKAG+S+DPAKIEAVT W RPSTVSEV SFL LAGYYRRF+ENF
Sbjct: 734 KCEFWLKQVSFLGHVVSKAGVSVDPAKIEAVTGWTRPSTVSEVRSFLGLAGYYRRFVENF 793

Query: 241 SRIATPLTQLTRKRASLVWSKACKDNF 268
           SRIATPLTQLTRK A  VWSKAC+D+F
Sbjct: 794 SRIATPLTQLTRKGAPFVWSKACEDSF 820

[Close]

BLAST of Cmc08g0221561 vs. NCBI nr
Match: KAA0058399.1 (pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 489.2 bits (1258), Expect = 2.3e-134
Identity = 240/267 (89.89%), Postives = 252/267 (94.38%), Query Frame = 0


Query: 1    MAPAELKELKVQLQELLDKGFIRPSVSPSGVPVLFVKKKDGSMRLCIDYRELNKVTVQNK 60
            MAPAELKELKVQLQELLDKGFIRPSVSP G PVLFVKKKDGSMRLCIDYRELNKVTV+N+
Sbjct: 811  MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKNR 870

Query: 61   YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKDSDVPKITFRSRYGHYEFIIMSFGLT 120
            YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKD DVPK  FRSRYGHYEFI+MSFGLT
Sbjct: 871  YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKDEDVPKTAFRSRYGHYEFIVMSFGLT 930

Query: 121  NAPIVFMDLMNRVFRELLDTFVIVFIDDILIYSKTEAEHKEHLRMVLETLRANELFAKFS 180
            NAP VFMDLMNRVFRE LDTFVIVFIDDILIYSKTEAEH+EHLRMVL+TLR N+L+AKFS
Sbjct: 931  NAPAVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLQTLRNNKLYAKFS 990

Query: 181  KCEFWLKQVSFLGHVVSKAGISMDPAKIEAVTSWPRPSTVSEVHSFLSLAGYYRRFIENF 240
            KCEFWLKQVSFLGHVVSKAG+S+DPAKIEAVT W RPSTVSEV SFL LAGYYRRF+ENF
Sbjct: 991  KCEFWLKQVSFLGHVVSKAGVSVDPAKIEAVTGWTRPSTVSEVRSFLGLAGYYRRFVENF 1050

Query: 241  SRIATPLTQLTRKRASLVWSKACKDNF 268
            SRIATPLTQLTRK A  VWSKAC+D+F
Sbjct: 1051 SRIATPLTQLTRKGAPFVWSKACEDSF 1077

[Close]

BLAST of Cmc08g0221561 vs. NCBI nr
Match: KAA0048687.1 (pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 489.2 bits (1258), Expect = 2.3e-134
Identity = 240/267 (89.89%), Postives = 252/267 (94.38%), Query Frame = 0


Query: 1   MAPAELKELKVQLQELLDKGFIRPSVSPSGVPVLFVKKKDGSMRLCIDYRELNKVTVQNK 60
           MAPAELKELKVQLQELLDKGFIRPSVSP G PVLFVKKKDGSMRLCIDYRELNKVTV+N+
Sbjct: 580 MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKNR 639

Query: 61  YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKDSDVPKITFRSRYGHYEFIIMSFGLT 120
           YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKD DVPK  FRSRYGHYEFI+MSFGLT
Sbjct: 640 YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKDEDVPKTAFRSRYGHYEFIVMSFGLT 699

Query: 121 NAPIVFMDLMNRVFRELLDTFVIVFIDDILIYSKTEAEHKEHLRMVLETLRANELFAKFS 180
           NAP VFMDLMNRVFRE LDTFVIVFIDDILIYSKTEAEH+EHLRMVL+TLR N+L+AKFS
Sbjct: 700 NAPAVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLQTLRDNKLYAKFS 759

Query: 181 KCEFWLKQVSFLGHVVSKAGISMDPAKIEAVTSWPRPSTVSEVHSFLSLAGYYRRFIENF 240
           KCEFWLKQVSFLGHVVSKAG+S+DPAKIEAVT W RPSTVSEV SFL LAGYYRRF+ENF
Sbjct: 760 KCEFWLKQVSFLGHVVSKAGVSVDPAKIEAVTGWTRPSTVSEVRSFLGLAGYYRRFVENF 819

Query: 241 SRIATPLTQLTRKRASLVWSKACKDNF 268
           SRIATPLTQLTRK A  VWSKAC+D+F
Sbjct: 820 SRIATPLTQLTRKGAPFVWSKACEDSF 846

[Close]

BLAST of Cmc08g0221561 vs. ExPASy Swiss-Prot
Match: P20825 (Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 5.4e-54
Identity = 100/255 (39.22%), Postives = 158/255 (61.96%), Query Frame = 0


Query: 8   ELKVQLQELLDKGFIRPSVSPSGVPVLFVKKKD-----GSMRLCIDYRELNKVTVQNKYP 67
           E++ Q+QE+L++G IR S SP   P   V KK         R+ IDYR+LN++T+ ++YP
Sbjct: 221 EVENQVQEMLNQGLIRESNSPYNSPTWVVPKKPDASGANKYRVVIDYRKLNEITIPDRYP 280

Query: 68  LPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKDSDVPKITFRSRYGHYEFIIMSFGLTNA 127
           +P +D++  +L     F+ IDL  G+HQ+ + +  + K  F ++ GHYE++ M FGL NA
Sbjct: 281 IPNMDEILGKLGKCQYFTTIDLAKGFHQIEMDEESISKTAFSTKSGHYEYLRMPFGLRNA 340

Query: 128 PIVFMDLMNRVFRELLDTFVIVFIDDILIYSKTEAEHKEHLRMVLETLRANELFAKFSKC 187
           P  F   MN + R LL+   +V++DDI+I+S +  EH   +++V   L    L  +  KC
Sbjct: 341 PATFQRCMNNILRPLLNKHCLVYLDDIIIFSTSLTEHLNSIQLVFTKLADANLKLQLDKC 400

Query: 188 EFWLKQVSFLGHVVSKAGISMDPAKIEAVTSWPRPSTVSEVHSFLSLAGYYRRFIENFSR 247
           EF  K+ +FLGH+V+  GI  +P K++A+ S+P P+   E+ +FL L GYYR+FI N++ 
Sbjct: 401 EFLKKEANFLGHIVTPDGIKPNPIKVKAIVSYPIPTKDKEIRAFLGLTGYYRKFIPNYAD 460

Query: 248 IATPLTQLTRKRASL 258
           IA P+T   +KR  +
Sbjct: 461 IAKPMTSCLKKRTKI 475

[Close]

BLAST of Cmc08g0221561 vs. ExPASy Swiss-Prot
Match: P04323 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 5.4e-54
Identity = 103/256 (40.23%), Postives = 163/256 (63.67%), Query Frame = 0


Query: 7   KELKVQLQELLDKGFIRPSVSPSGVPVLFV-KKKDGS----MRLCIDYRELNKVTVQNKY 66
           +E++ Q+Q++L++G IR S SP   P+  V KK+D S     R+ IDYR+LN++TV +++
Sbjct: 221 QEVESQIQDMLNQGIIRTSNSPYNSPIWVVPKKQDASGKQKFRIVIDYRKLNEITVGDRH 280

Query: 67  PLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKDSDVPKITFRSRYGHYEFIIMSFGLTN 126
           P+P +D++  +L     F+ IDL  G+HQ+ +    V K  F +++GHYE++ M FGL N
Sbjct: 281 PIPNMDEILGKLGRCNYFTTIDLAKGFHQIEMDPESVSKTAFSTKHGHYEYLRMPFGLKN 340

Query: 127 APIVFMDLMNRVFRELLDTFVIVFIDDILIYSKTEAEHKEHLRMVLETLRANELFAKFSK 186
           AP  F   MN + R LL+   +V++DDI+++S +  EH + L +V E L    L  +  K
Sbjct: 341 APATFQRCMNDILRPLLNKHCLVYLDDIIVFSTSLDEHLQSLGLVFEKLAKANLKLQLDK 400

Query: 187 CEFWLKQVSFLGHVVSKAGISMDPAKIEAVTSWPRPSTVSEVHSFLSLAGYYRRFIENFS 246
           CEF  ++ +FLGHV++  GI  +P KIEA+  +P P+   E+ +FL L GYYR+FI NF+
Sbjct: 401 CEFLKQETTFLGHVLTPDGIKPNPEKIEAIQKYPIPTKPKEIKAFLGLTGYYRKFIPNFA 460

Query: 247 RIATPLTQLTRKRASL 258
            IA P+T+  +K   +
Sbjct: 461 DIAKPMTKCLKKNMKI 476

[Close]

BLAST of Cmc08g0221561 vs. ExPASy Swiss-Prot
Match: Q99315 (Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-G PE=1 SV=3)

HSP 1 Score: 209.9 bits (533), Expect = 3.5e-53
Identity = 107/241 (44.40%), Postives = 149/241 (61.83%), Query Frame = 0


Query: 7   KELKVQLQELLDKGFIRPSVSPSGVPVLFVKKKDGSMRLCIDYRELNKVTVQNKYPLPRI 66
           +E+   +Q+LLD  FI PS SP   PV+ V KKDG+ RLC+DYR LNK T+ + +PLPRI
Sbjct: 610 QEINKIVQKLLDNKFIVPSKSPCSSPVVLVPKKDGTFRLCVDYRTLNKATISDPFPLPRI 669

Query: 67  DDLFDQLQGATVFSKIDLRSGYHQLRIKDSDVPKITFRSRYGHYEFIIMSFGLTNAPIVF 126
           D+L  ++  A +F+ +DL SGYHQ+ ++  D  K  F +  G YE+ +M FGL NAP  F
Sbjct: 670 DNLLSRIGNAQIFTTLDLHSGYHQIPMEPKDRYKTAFVTPSGKYEYTVMPFGLVNAPSTF 729

Query: 127 MDLMNRVFRELLDTFVIVFIDDILIYSKTEAEHKEHLRMVLETLRANELFAKFSKCEFWL 186
              M   FR+L   FV V++DDILI+S++  EH +HL  VLE L+   L  K  KC+F  
Sbjct: 730 ARYMADTFRDL--RFVNVYLDDILIFSESPEEHWKHLDTVLERLKNENLIVKKKKCKFAS 789

Query: 187 KQVSFLGHVVSKAGISMDPAKIEAVTSWPRPSTVSEVHSFLSLAGYYRRFIENFSRIATP 246
           ++  FLG+ +    I+    K  A+  +P P TV +   FL +  YYRRFI N S+IA P
Sbjct: 790 EETEFLGYSIGIQKIAPLQHKCAAIRDFPTPKTVKQAQRFLGMINYYRRFIPNCSKIAQP 848

Query: 247 L 248
           +
Sbjct: 850 I 848

[Close]

BLAST of Cmc08g0221561 vs. ExPASy Swiss-Prot
Match: Q7LHG5 (Transposon Ty3-I Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-I PE=1 SV=2)

HSP 1 Score: 209.9 bits (533), Expect = 3.5e-53
Identity = 107/241 (44.40%), Postives = 149/241 (61.83%), Query Frame = 0


Query: 7   KELKVQLQELLDKGFIRPSVSPSGVPVLFVKKKDGSMRLCIDYRELNKVTVQNKYPLPRI 66
           +E+   +Q+LLD  FI PS SP   PV+ V KKDG+ RLC+DYR LNK T+ + +PLPRI
Sbjct: 636 QEINKIVQKLLDNKFIVPSKSPCSSPVVLVPKKDGTFRLCVDYRTLNKATISDPFPLPRI 695

Query: 67  DDLFDQLQGATVFSKIDLRSGYHQLRIKDSDVPKITFRSRYGHYEFIIMSFGLTNAPIVF 126
           D+L  ++  A +F+ +DL SGYHQ+ ++  D  K  F +  G YE+ +M FGL NAP  F
Sbjct: 696 DNLLSRIGNAQIFTTLDLHSGYHQIPMEPKDRYKTAFVTPSGKYEYTVMPFGLVNAPSTF 755

Query: 127 MDLMNRVFRELLDTFVIVFIDDILIYSKTEAEHKEHLRMVLETLRANELFAKFSKCEFWL 186
              M   FR+L   FV V++DDILI+S++  EH +HL  VLE L+   L  K  KC+F  
Sbjct: 756 ARYMADTFRDL--RFVNVYLDDILIFSESPEEHWKHLDTVLERLKNENLIVKKKKCKFAS 815

Query: 187 KQVSFLGHVVSKAGISMDPAKIEAVTSWPRPSTVSEVHSFLSLAGYYRRFIENFSRIATP 246
           ++  FLG+ +    I+    K  A+  +P P TV +   FL +  YYRRFI N S+IA P
Sbjct: 816 EETEFLGYSIGIQKIAPLQHKCAAIRDFPTPKTVKQAQRFLGMINYYRRFIPNCSKIAQP 874

Query: 247 L 248
           +
Sbjct: 876 I 874

[Close]

BLAST of Cmc08g0221561 vs. ExPASy Swiss-Prot
Match: Q8I7P9 (Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 2.5e-51
Identity = 99/250 (39.60%), Postives = 154/250 (61.60%), Query Frame = 0


Query: 8   ELKVQLQELLDKGFIRPSVSPSGVPVLFVKKK-----DGSMRLCIDYRELNKVTVQNKYP 67
           E++ Q+ ELL  G IRPS SP   P+  V KK     +   R+ +D++ LN VT+ + YP
Sbjct: 138 EVERQIDELLQDGIIRPSNSPYNSPIWIVPKKPKPNGEKQYRMVVDFKRLNTVTIPDTYP 197

Query: 68  LPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKDSDVPKITFRSRYGHYEFIIMSFGLTNA 127
           +P I+     L  A  F+ +DL SG+HQ+ +K+SD+PK  F +  G YEF+ + FGL NA
Sbjct: 198 IPDINATLASLGNAKYFTTLDLTSGFHQIHMKESDIPKTAFSTLNGKYEFLRLPFGLKNA 257

Query: 128 PIVFMDLMNRVFRELLDTFVIVFIDDILIYSKTEAEHKEHLRMVLETLRANELFAKFSKC 187
           P +F  +++ + RE +     V+IDDI+++S+    H ++LR+VL +L    L     K 
Sbjct: 258 PAIFQRMIDDILREHIGKVCYVYIDDIIVFSEDYDTHWKNLRLVLASLSKANLQVNLEKS 317

Query: 188 EFWLKQVSFLGHVVSKAGISMDPAKIEAVTSWPRPSTVSEVHSFLSLAGYYRRFIENFSR 247
            F   QV FLG++V+  GI  DP K+ A++  P P++V E+  FL +  YYR+FI+++++
Sbjct: 318 HFLDTQVEFLGYIVTADGIKADPKKVRAISEMPPPTSVKELKRFLGMTSYYRKFIQDYAK 377

Query: 248 IATPLTQLTR 253
           +A PLT LTR
Sbjct: 378 VAKPLTNLTR 387

[Close]

BLAST of Cmc08g0221561 vs. ExPASy TrEMBL
Match: A0A5A7UAV9 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold207G001870 PE=4 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 5.0e-135
Identity = 239/267 (89.51%), Postives = 254/267 (95.13%), Query Frame = 0


Query: 1   MAPAELKELKVQLQELLDKGFIRPSVSPSGVPVLFVKKKDGSMRLCIDYRELNKVTVQNK 60
           MAPAELKELKVQLQELLDKGFIR SVSP G PVLFVKKKDGSMRLCIDYRELNKVTV+N+
Sbjct: 456 MAPAELKELKVQLQELLDKGFIRSSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKNR 515

Query: 61  YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKDSDVPKITFRSRYGHYEFIIMSFGLT 120
           YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKD DVPKI FRSRYGHYEFI+MSFGLT
Sbjct: 516 YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKDGDVPKIAFRSRYGHYEFIVMSFGLT 575

Query: 121 NAPIVFMDLMNRVFRELLDTFVIVFIDDILIYSKTEAEHKEHLRMVLETLRANELFAKFS 180
           NAP VFMDLMNRVFRE LDTFVIVFIDDILIYSKTEAEH+EHLRMVL+TLR N+L+AKFS
Sbjct: 576 NAPTVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLQTLRDNKLYAKFS 635

Query: 181 KCEFWLKQVSFLGHVVSKAGISMDPAKIEAVTSWPRPSTVSEVHSFLSLAGYYRRFIENF 240
           KCEFWLKQVSFLGHVVSKAG+ +DPAKIEA+TSWPRP TVS+VHSFL LAGYYR+F+ENF
Sbjct: 636 KCEFWLKQVSFLGHVVSKAGVYVDPAKIEAITSWPRPFTVSKVHSFLGLAGYYRQFVENF 695

Query: 241 SRIATPLTQLTRKRASLVWSKACKDNF 268
           SRIATPLTQLTRKRA  VWSKAC+D+F
Sbjct: 696 SRIATPLTQLTRKRAPFVWSKACEDSF 722

[Close]

BLAST of Cmc08g0221561 vs. ExPASy TrEMBL
Match: A0A5D3BQD5 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold113G001680 PE=4 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 5.0e-135
Identity = 239/267 (89.51%), Postives = 254/267 (95.13%), Query Frame = 0


Query: 1   MAPAELKELKVQLQELLDKGFIRPSVSPSGVPVLFVKKKDGSMRLCIDYRELNKVTVQNK 60
           MAPAELKELKVQLQELLDKGFIR SVSP G PVLFVKKKDGSMRLCIDYRELNKVTV+N+
Sbjct: 456 MAPAELKELKVQLQELLDKGFIRSSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKNR 515

Query: 61  YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKDSDVPKITFRSRYGHYEFIIMSFGLT 120
           YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKD DVPKI FRSRYGHYEFI+MSFGLT
Sbjct: 516 YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKDGDVPKIAFRSRYGHYEFIVMSFGLT 575

Query: 121 NAPIVFMDLMNRVFRELLDTFVIVFIDDILIYSKTEAEHKEHLRMVLETLRANELFAKFS 180
           NAP VFMDLMNRVFRE LDTFVIVFIDDILIYSKTEAEH+EHLRMVL+TLR N+L+AKFS
Sbjct: 576 NAPTVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLQTLRDNKLYAKFS 635

Query: 181 KCEFWLKQVSFLGHVVSKAGISMDPAKIEAVTSWPRPSTVSEVHSFLSLAGYYRRFIENF 240
           KCEFWLKQVSFLGHVVSKAG+ +DPAKIEA+TSWPRP TVS+VHSFL LAGYYR+F+ENF
Sbjct: 636 KCEFWLKQVSFLGHVVSKAGVYVDPAKIEAITSWPRPFTVSKVHSFLGLAGYYRQFVENF 695

Query: 241 SRIATPLTQLTRKRASLVWSKACKDNF 268
           SRIATPLTQLTRKRA  VWSKAC+D+F
Sbjct: 696 SRIATPLTQLTRKRAPFVWSKACEDSF 722

[Close]

BLAST of Cmc08g0221561 vs. ExPASy TrEMBL
Match: A0A5A7T9B7 (Gag protease polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold270G00730 PE=4 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 8.4e-135
Identity = 240/267 (89.89%), Postives = 252/267 (94.38%), Query Frame = 0


Query: 1   MAPAELKELKVQLQELLDKGFIRPSVSPSGVPVLFVKKKDGSMRLCIDYRELNKVTVQNK 60
           MAPAELKELKVQLQELLDKGFIRPSVSP G PVLFVKKKDGSMRLCIDYRELNKVTV+N+
Sbjct: 554 MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKNR 613

Query: 61  YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKDSDVPKITFRSRYGHYEFIIMSFGLT 120
           YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKD DVPK  FRSRYGHYEFI+MSFGLT
Sbjct: 614 YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKDEDVPKTAFRSRYGHYEFIVMSFGLT 673

Query: 121 NAPIVFMDLMNRVFRELLDTFVIVFIDDILIYSKTEAEHKEHLRMVLETLRANELFAKFS 180
           NAP VFMDLMNRVFRE LDTFVIVFIDDILIYSKTEAEHKEHLRMVL+TLR N+L++KFS
Sbjct: 674 NAPAVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHKEHLRMVLQTLRDNKLYSKFS 733

Query: 181 KCEFWLKQVSFLGHVVSKAGISMDPAKIEAVTSWPRPSTVSEVHSFLSLAGYYRRFIENF 240
           KCEFWLKQVSFLGHVVSKAG+S+DPAKIEAVT W RPSTVSEV SFL LAGYYRRF+ENF
Sbjct: 734 KCEFWLKQVSFLGHVVSKAGVSVDPAKIEAVTGWTRPSTVSEVRSFLGLAGYYRRFVENF 793

Query: 241 SRIATPLTQLTRKRASLVWSKACKDNF 268
           SRIATPLTQLTRK A  VWSKAC+D+F
Sbjct: 794 SRIATPLTQLTRKGAPFVWSKACEDSF 820

[Close]

BLAST of Cmc08g0221561 vs. ExPASy TrEMBL
Match: A0A5A7TXE4 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold157G00060 PE=4 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 1.1e-134
Identity = 240/267 (89.89%), Postives = 253/267 (94.76%), Query Frame = 0


Query: 1   MAPAELKELKVQLQELLDKGFIRPSVSPSGVPVLFVKKKDGSMRLCIDYRELNKVTVQNK 60
           MAPAELKELKVQLQELLDKGFIRPSVSP G PVLFVKKKDGSMRLCIDYRELNKVTV+N+
Sbjct: 377 MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKNR 436

Query: 61  YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKDSDVPKITFRSRYGHYEFIIMSFGLT 120
           YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKD DVPK  FRSRYGHYEFI+MSFGLT
Sbjct: 437 YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKDEDVPKTAFRSRYGHYEFIVMSFGLT 496

Query: 121 NAPIVFMDLMNRVFRELLDTFVIVFIDDILIYSKTEAEHKEHLRMVLETLRANELFAKFS 180
           NAP VFMDLMNRVFRE LDTFVIVFIDDILIYSKTEAEH+EHLRMVL+TLR N+L+AKFS
Sbjct: 497 NAPAVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLQTLRDNKLYAKFS 556

Query: 181 KCEFWLKQVSFLGHVVSKAGISMDPAKIEAVTSWPRPSTVSEVHSFLSLAGYYRRFIENF 240
           KCEFWLKQVSFLGHVVSKAG+S+DPAKIEAVT W RPSTVSEV SFLSLAGYYRRF+ENF
Sbjct: 557 KCEFWLKQVSFLGHVVSKAGVSVDPAKIEAVTGWTRPSTVSEVRSFLSLAGYYRRFVENF 616

Query: 241 SRIATPLTQLTRKRASLVWSKACKDNF 268
           SRIATPLTQLTRK A  VWSKAC+++F
Sbjct: 617 SRIATPLTQLTRKGAPFVWSKACEESF 643

[Close]

BLAST of Cmc08g0221561 vs. ExPASy TrEMBL
Match: A0A5D3BPI1 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold451G002120 PE=4 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 1.1e-134
Identity = 240/267 (89.89%), Postives = 252/267 (94.38%), Query Frame = 0


Query: 1    MAPAELKELKVQLQELLDKGFIRPSVSPSGVPVLFVKKKDGSMRLCIDYRELNKVTVQNK 60
            MAPAELKELKVQLQELLDKGFIRPSVSP G PVLFVKKKDGSMRLCIDYRELNKVTV+N+
Sbjct: 834  MAPAELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRELNKVTVKNR 893

Query: 61   YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKDSDVPKITFRSRYGHYEFIIMSFGLT 120
            YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKD DVPK  FRSRYGHYEFI+MSFGLT
Sbjct: 894  YPLPRIDDLFDQLQGATVFSKIDLRSGYHQLRIKDEDVPKTAFRSRYGHYEFIVMSFGLT 953

Query: 121  NAPIVFMDLMNRVFRELLDTFVIVFIDDILIYSKTEAEHKEHLRMVLETLRANELFAKFS 180
            NAP VFMDLMNRVFRE LDTFVIVFIDDILIYSKTEAEH+EHLRMVL+TLR N+L+AKFS
Sbjct: 954  NAPAVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLQTLRDNKLYAKFS 1013

Query: 181  KCEFWLKQVSFLGHVVSKAGISMDPAKIEAVTSWPRPSTVSEVHSFLSLAGYYRRFIENF 240
            KCEFWLKQVSFLGHVVSKAG+S+DPAKIEAVT W RPSTVSEV SFL LAGYYRRF+ENF
Sbjct: 1014 KCEFWLKQVSFLGHVVSKAGVSVDPAKIEAVTGWTRPSTVSEVRSFLGLAGYYRRFVENF 1073

Query: 241  SRIATPLTQLTRKRASLVWSKACKDNF 268
            SRIATPLTQLTRK A  VWSKAC+D+F
Sbjct: 1074 SRIATPLTQLTRKGAPFVWSKACEDSF 1100

[Close]

BLAST of Cmc08g0221561 vs. TAIR 10
Match: ATMG00860.1 (DNA/RNA polymerases superfamily protein )

HSP 1 Score: 99.8 bits (247), Expect = 3.6e-21
Identity = 45/102 (44.12%), Postives = 67/102 (65.69%), Query Frame = 0


Query: 162 HLRMVLETLRANELFAKFSKCEFWLKQVSFLG--HVVSKAGISMDPAKIEAVTSWPRPST 221
           HL MVL+    ++ +A   KC F   Q+++LG  H++S  G+S DPAK+EA+  WP P  
Sbjct: 3   HLGMVLQIWEQHQFYANRKKCAFGQPQIAYLGHRHIISGEGVSADPAKLEAMVGWPEPKN 62

Query: 222 VSEVHSFLSLAGYYRRFIENFSRIATPLTQLTRKRASLVWSK 262
            +E+  FL L GYYRRF++N+ +I  PLT+L +K  SL W++
Sbjct: 63  TTELRGFLGLTGYYRRFVKNYGKIVRPLTELLKKN-SLKWTE 103

The following BLAST results are available for this feature:

BLAST of Cmc08g0221561 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0052348.1	1.0e-134	89.51	putative gag-pol polyprotein [Cucumis melo var. makuwa]	[more]
TYK01903.1	1.0e-134	89.51	putative gag-pol polyprotein [Cucumis melo var. makuwa]	[more]
KAA0038231.1	1.7e-134	89.89	gag protease polyprotein [Cucumis melo var. makuwa]	[more]
KAA0058399.1	2.3e-134	89.89	pol protein [Cucumis melo var. makuwa]	[more]
KAA0048687.1	2.3e-134	89.89	pol protein [Cucumis melo var. makuwa]	[more]

BLAST of Cmc08g0221561 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P20825	5.4e-54	39.22	Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaste...	[more]
P04323	5.4e-54	40.23	Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogast...	[more]
Q99315	3.5e-53	44.40	Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 20...	[more]
Q7LHG5	3.5e-53	44.40	Transposon Ty3-I Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 20...	[more]
Q8I7P9	2.5e-51	39.60	Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogast...	[more]

BLAST of Cmc08g0221561 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UAV9	5.0e-135	89.51	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold20...	[more]
A0A5D3BQD5	5.0e-135	89.51	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold11...	[more]
A0A5A7T9B7	8.4e-135	89.89	Gag protease polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol...	[more]
A0A5A7TXE4	1.1e-134	89.89	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold15...	[more]
A0A5D3BPI1	1.1e-134	89.89	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold45...	[more]

BLAST of Cmc08g0221561 vs. TAIR 10


Match Name	E-value	Identity	Description
ATMG00860.1	3.6e-21	44.12	DNA/RNA polymerases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000477	Reverse transcriptase domain	PFAM	PF00078	RVT_1	coord: 36..195 e-value: 1.3E-27 score: 96.7
IPR000477	Reverse transcriptase domain	PROSITE	PS50878	RT_POL	coord: 17..196 score: 10.085058
IPR043128	Reverse transcriptase/Diguanylate cyclase domain	GENE3D	3.30.70.270		coord: 206..267 e-value: 5.2E-21 score: 76.5
IPR043128	Reverse transcriptase/Diguanylate cyclase domain	GENE3D	3.30.70.270		coord: 61..196 e-value: 2.1E-83 score: 280.8
None	No IPR available	GENE3D	3.10.10.10	HIV Type 1 Reverse Transcriptase, subunit A, domain 1	coord: 1..121 e-value: 2.1E-83 score: 280.8
None	No IPR available	PANTHER	PTHR24559	TRANSPOSON TY3-I GAG-POL POLYPROTEIN	coord: 2..254
None	No IPR available	PANTHER	PTHR24559:SF324	TRANSPOSON TY3-I GAG-POL POLYPROTEIN-LIKE PROTEIN	coord: 2..254
None	No IPR available	CDD	cd01647	RT_LTR	coord: 20..196 e-value: 1.3883E-90 score: 264.072
IPR043502	DNA/RNA polymerase superfamily	SUPERFAMILY	56672	DNA/RNA polymerases	coord: 2..267

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cmc08g0221561.1	Cmc08g0221561.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
biological_process	GO:0006508	proteolysis
biological_process	GO:0006355	regulation of transcription, DNA-templated
biological_process	GO:0006278	RNA-dependent DNA biosynthetic process
cellular_component	GO:0016020	membrane
molecular_function	GO:0004190	aspartic-type endopeptidase activity
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0003964	RNA-directed DNA polymerase activity
molecular_function	GO:0043565	sequence-specific DNA binding
molecular_function	GO:0008270	zinc ion binding