Cmc08g0218921 (gene) Melon (Charmono) v1.1

Overview


Name	Cmc08g0218921
Type	gene
Organism	Cucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Description	WAT1-related protein
Location	CMiso1.1chr08: 7098340 .. 7098958 (-)
RNA-Seq Expression	Cmc08g0218921
Synteny	Cmc08g0218921

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CATTAACATGTGATGAGGCAGAAAAATTCCCCTTATTCTAGTGGGAAAAAAGAAAAATGCAAAACACACATTTTTGTTATATATATATATATATATATATATGTCATATGTATGTAGAAGGTAGTTTGATAAGAGAAAAAAGAATGGAACTAAAGAAGCCTCTGATTGGAGCCATTGTTGTTCAAATCACATACTCTGGAATGAGTATTTTGGCAAAAGCTGCTTTTACTTCTGGTATGAACAACTTCATTTTTATTTTTTATAGGCAAGCTTTTGGAACTCTCTTCCTTATCCCTCCTACCATCCTTTTCAAAAGGTAATTAATTTTCTAATTTTAAATTTATTAATCACTATTTTCATACAACATTTCTTTCGAATAACACTTGTTTTTTTTTGTATGGATGTGTGGTAGAAAAGAAGTAGCATGTTTATCGATAGGAGATATGTTCAAGATTTTCATGTTGGCTTTACTTGGGTAATTTTTACAACTTTTTTTTTTCTTTTTTCTTTTTAAGATCAGAAAAGAAATTTAGTAATTAATTGAGAAATGCATGCAGGAGGATGTTGGTGTTGATAGCTTATAGTTTAGGTGTTAAATATACATCTGCAGTTTCTGGTG

mRNA sequence

CATTAACATGTGATGAGGCAGAAAAATTCCCCTTATTCTAGTGGGAAAAAAGAAAAATGCAAAACACACATTTTTGTTATATATATATATATATATATATATGTCATATGTATGTAGAAGGTAGTTTGATAAGAGAAAAAAGAATGGAACTAAAGAAGCCTCTGATTGGAGCCATTGTTGTTCAAATCACATACTCTGGAATGAGTATTTTGGCAAAAGCTGCTTTTACTTCTGGTATGAACAACTTCATTTTTATTTTTTATAGGCAAGCTTTTGGAACTCTCTTCCTTATCCCTCCTACCATCCTTTTCAAAAGGTAATTAATTTTCTAATTTTAAATTTATTAATCACTATTTTCATACAACATTTCTTTCGAATAACACTTGTTTTTTTTTGTATGGATGTGTGGTAGAAAAGAAGTAGCATGTTTATCGATAGGAGATATGTTCAAGATTTTCATGTTGGCTTTACTTGGGAGGATGTTGGTGTTGATAGCTTATAGTTTAGGTGTTAAATATACATCTGCAGTTTCTGGTG

Coding sequence (CDS)

ATGCAAAACACACATTTTTGTTATATATATATATATATATATATATGTCATATGTATGTAGAAGGTAGTTTGATAAGAGAAAAAAGAATGGAACTAAAGAAGCCTCTGATTGGAGCCATTGTTGTTCAAATCACATACTCTGGAATGAGTATTTTGGCAAAAGCTGCTTTTACTTCTGGTATGAACAACTTCATTTTTATTTTTTATAGGCAAGCTTTTGGAACTCTCTTCCTTATCCCTCCTACCATCCTTTTCAAAAGGTAA

Protein sequence

MQNTHFCYIYIYIYICHMYVEGSLIREKRMELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLIPPTILFKR

Homology

[Close]

BLAST of Cmc08g0218921 vs. NCBI nr
Match: KAE8647468.1 (hypothetical protein Csa_003273 [Cucumis sativus])

HSP 1 Score: 136.7 bits (343), Expect = 9.4e-29
Identity = 70/82 (85.37%), Postives = 77/82 (93.90%), Query Frame = 0


Query: 6   FCYIYIYIYICHMYVEGSLIREKRMELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFI 65
           + + YIY+ +C +YVEG+ +REKRMELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFI
Sbjct: 26  YLFCYIYVSVC-VYVEGNFMREKRMELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFI 85

Query: 66  FIFYRQAFGTLFLIPPTILFKR 88
           FIFYRQAFGTLFLIPPTILFKR
Sbjct: 86  FIFYRQAFGTLFLIPPTILFKR 106

[Close]

BLAST of Cmc08g0218921 vs. NCBI nr
Match: XP_008441011.1 (PREDICTED: WAT1-related protein At5g64700-like [Cucumis melo])

HSP 1 Score: 136.7 bits (343), Expect = 9.4e-29
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = 0


Query: 18 MYVEGSLIREKRMELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLF 77
          MYVEGSLIREKRMELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLF
Sbjct: 1  MYVEGSLIREKRMELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLF 60

Query: 78 LIPPTILFKR 88
          LIPPTILFKR
Sbjct: 61 LIPPTILFKR 70

[Close]

BLAST of Cmc08g0218921 vs. NCBI nr
Match: XP_011657768.2 (WAT1-related protein At5g64700 [Cucumis sativus])

HSP 1 Score: 122.1 bits (305), Expect = 2.4e-24
Identity = 62/63 (98.41%), Postives = 63/63 (100.00%), Query Frame = 0


Query: 25 IREKRMELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLIPPTIL 84
          +REKRMELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLIPPTIL
Sbjct: 1  MREKRMELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLIPPTIL 60

Query: 85 FKR 88
          FKR
Sbjct: 61 FKR 63

[Close]

BLAST of Cmc08g0218921 vs. NCBI nr
Match: KAA0025549.1 (WAT1-related protein [Cucumis melo var. makuwa])

HSP 1 Score: 113.2 bits (282), Expect = 1.1e-21
Identity = 58/58 (100.00%), Postives = 58/58 (100.00%), Query Frame = 0


Query: 30 MELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLIPPTILFKR 88
          MELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLIPPTILFKR
Sbjct: 1  MELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLIPPTILFKR 58

[Close]

BLAST of Cmc08g0218921 vs. NCBI nr
Match: KAA0053277.1 (WAT1-related protein [Cucumis melo var. makuwa])

HSP 1 Score: 111.3 bits (277), Expect = 4.2e-21
Identity = 56/58 (96.55%), Postives = 57/58 (98.28%), Query Frame = 0


Query: 30 MELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLIPPTILFKR 88
          MELKKPLIG +VVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLIPPTILFKR
Sbjct: 1  MELKKPLIGVVVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLIPPTILFKR 58

[Close]

BLAST of Cmc08g0218921 vs. ExPASy Swiss-Prot
Match: Q9FGG3 (WAT1-related protein At5g64700 OS=Arabidopsis thaliana OX=3702 GN=At5g64700 PE=2 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 7.7e-10
Identity = 28/58 (48.28%), Postives = 40/58 (68.97%), Query Frame = 0


Query: 30 MELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLIPPTILFKR 88
          ME KKP +   ++Q+ Y+ M +++KA F  GMN F+F+FYRQAF T+FL P    F+R
Sbjct: 3  MESKKPYLMVTIIQVIYTIMFLISKAVFNGGMNTFVFVFYRQAFATIFLAPLAFFFER 60

[Close]

BLAST of Cmc08g0218921 vs. ExPASy Swiss-Prot
Match: Q6NMB7 (WAT1-related protein At1g43650 OS=Arabidopsis thaliana OX=3702 GN=At1g43650 PE=2 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 2.6e-05
Identity = 23/47 (48.94%), Postives = 31/47 (65.96%), Query Frame = 0


Query: 34 KPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLIP 81
          K  +  + VQI Y+GM +L+K A + G N F+F+FYRQAF  L L P
Sbjct: 6  KANMAMVFVQIVYAGMPLLSKVAISQGTNPFVFVFYRQAFAALALSP 52

[Close]

BLAST of Cmc08g0218921 vs. ExPASy Swiss-Prot
Match: Q9ZUS1 (WAT1-related protein At2g37460 OS=Arabidopsis thaliana OX=3702 GN=At2g37460 PE=2 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 7.5e-05
Identity = 21/58 (36.21%), Postives = 34/58 (58.62%), Query Frame = 0


Query: 30 MELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLIPPTILFKR 88
          ME  +P I  +V+Q+  +GM IL+KA    GM+N++ + YR A  T+ + P    F +
Sbjct: 10 MEKARPFISMVVLQVGLAGMDILSKAVLNKGMSNYVLVVYRHAVATIVMAPFAFYFDK 67

[Close]

BLAST of Cmc08g0218921 vs. ExPASy Swiss-Prot
Match: F4I5D5 (WAT1-related protein At1g70260 OS=Arabidopsis thaliana OX=3702 GN=At1g70260 PE=2 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 2.8e-04
Identity = 22/63 (34.92%), Postives = 39/63 (61.90%), Query Frame = 0


Query: 25 IREKRMELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLIPPTIL 84
          ++ +R EL  P +   +++     ++I+AK A T GM+ F+F+ Y  AFG++ L+P + L
Sbjct: 3  VKVRRDEL-VPFVAMAIMEACTIALTIMAKTALTGGMSPFVFVVYTNAFGSILLLPFSFL 62

Query: 85 FKR 88
          F R
Sbjct: 63 FHR 64

[Close]

BLAST of Cmc08g0218921 vs. ExPASy Swiss-Prot
Match: Q9SUD5 (WAT1-related protein At4g28040 OS=Arabidopsis thaliana OX=3702 GN=At4g28040 PE=2 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 2.8e-04
Identity = 19/47 (40.43%), Postives = 32/47 (68.09%), Query Frame = 0


Query: 34 KPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLIP 81
          K ++  +++Q T +G+++  KAAF  G+N  +F+ YRQA  TLF+ P
Sbjct: 7  KAVLALVMLQFTSAGVALFTKAAFMEGLNPTVFVVYRQAIATLFICP 53

[Close]

BLAST of Cmc08g0218921 vs. ExPASy TrEMBL
Match: A0A1S3B338 (WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103485253 PE=3 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 4.5e-29
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = 0


Query: 18 MYVEGSLIREKRMELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLF 77
          MYVEGSLIREKRMELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLF
Sbjct: 1  MYVEGSLIREKRMELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLF 60

Query: 78 LIPPTILFKR 88
          LIPPTILFKR
Sbjct: 61 LIPPTILFKR 70

[Close]

BLAST of Cmc08g0218921 vs. ExPASy TrEMBL
Match: A0A5A7SJQ3 (WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold253G00380 PE=3 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 5.4e-22
Identity = 58/58 (100.00%), Postives = 58/58 (100.00%), Query Frame = 0


Query: 30 MELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLIPPTILFKR 88
          MELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLIPPTILFKR
Sbjct: 1  MELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLIPPTILFKR 58

[Close]

BLAST of Cmc08g0218921 vs. ExPASy TrEMBL
Match: A0A0A0KFR8 (WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_6G484610 PE=3 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 5.4e-22
Identity = 58/58 (100.00%), Postives = 58/58 (100.00%), Query Frame = 0


Query: 30 MELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLIPPTILFKR 88
          MELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLIPPTILFKR
Sbjct: 1  MELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLIPPTILFKR 58

[Close]

BLAST of Cmc08g0218921 vs. ExPASy TrEMBL
Match: A0A5A7UFK5 (WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold102G00780 PE=3 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 2.0e-21
Identity = 56/58 (96.55%), Postives = 57/58 (98.28%), Query Frame = 0


Query: 30 MELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLIPPTILFKR 88
          MELKKPLIG +VVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLIPPTILFKR
Sbjct: 1  MELKKPLIGVVVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLIPPTILFKR 58

[Close]

BLAST of Cmc08g0218921 vs. ExPASy TrEMBL
Match: A0A5A7T859 (WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold767G00290 PE=4 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 7.7e-21
Identity = 57/58 (98.28%), Postives = 57/58 (98.28%), Query Frame = 0


Query: 30 MELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLIPPTILFKR 88
          MELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLI PTILFKR
Sbjct: 1  MELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLILPTILFKR 58

[Close]

BLAST of Cmc08g0218921 vs. TAIR 10
Match: AT5G64700.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 64.3 bits (155), Expect = 5.5e-11
Identity = 28/58 (48.28%), Postives = 40/58 (68.97%), Query Frame = 0


Query: 30 MELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLIPPTILFKR 88
          ME KKP +   ++Q+ Y+ M +++KA F  GMN F+F+FYRQAF T+FL P    F+R
Sbjct: 3  MESKKPYLMVTIIQVIYTIMFLISKAVFNGGMNTFVFVFYRQAFATIFLAPLAFFFER 60

[Close]

BLAST of Cmc08g0218921 vs. TAIR 10
Match: AT1G43650.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 49.3 bits (116), Expect = 1.8e-06
Identity = 23/47 (48.94%), Postives = 31/47 (65.96%), Query Frame = 0


Query: 34 KPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLIP 81
          K  +  + VQI Y+GM +L+K A + G N F+F+FYRQAF  L L P
Sbjct: 6  KANMAMVFVQIVYAGMPLLSKVAISQGTNPFVFVFYRQAFAALALSP 52

[Close]

BLAST of Cmc08g0218921 vs. TAIR 10
Match: AT1G43650.2 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 49.3 bits (116), Expect = 1.8e-06
Identity = 23/47 (48.94%), Postives = 31/47 (65.96%), Query Frame = 0


Query: 34 KPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLIP 81
          K  +  + VQI Y+GM +L+K A + G N F+F+FYRQAF  L L P
Sbjct: 6  KANMAMVFVQIVYAGMPLLSKVAISQGTNPFVFVFYRQAFAALALSP 52

[Close]

BLAST of Cmc08g0218921 vs. TAIR 10
Match: AT2G37460.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 47.8 bits (112), Expect = 5.3e-06
Identity = 21/58 (36.21%), Postives = 34/58 (58.62%), Query Frame = 0


Query: 30 MELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLIPPTILFKR 88
          ME  +P I  +V+Q+  +GM IL+KA    GM+N++ + YR A  T+ + P    F +
Sbjct: 10 MEKARPFISMVVLQVGLAGMDILSKAVLNKGMSNYVLVVYRHAVATIVMAPFAFYFDK 67

[Close]

BLAST of Cmc08g0218921 vs. TAIR 10
Match: AT1G70260.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 45.8 bits (107), Expect = 2.0e-05
Identity = 22/63 (34.92%), Postives = 39/63 (61.90%), Query Frame = 0


Query: 25 IREKRMELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLIPPTIL 84
          ++ +R EL  P +   +++     ++I+AK A T GM+ F+F+ Y  AFG++ L+P + L
Sbjct: 3  VKVRRDEL-VPFVAMAIMEACTIALTIMAKTALTGGMSPFVFVVYTNAFGSILLLPFSFL 62

Query: 85 FKR 88
          F R
Sbjct: 63 FHR 64

The following BLAST results are available for this feature:

BLAST of Cmc08g0218921 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8647468.1	9.4e-29	85.37	hypothetical protein Csa_003273 [Cucumis sativus]	[more]
XP_008441011.1	9.4e-29	100.00	PREDICTED: WAT1-related protein At5g64700-like [Cucumis melo]	[more]
XP_011657768.2	2.4e-24	98.41	WAT1-related protein At5g64700 [Cucumis sativus]	[more]
KAA0025549.1	1.1e-21	100.00	WAT1-related protein [Cucumis melo var. makuwa]	[more]
KAA0053277.1	4.2e-21	96.55	WAT1-related protein [Cucumis melo var. makuwa]	[more]

BLAST of Cmc08g0218921 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FGG3	7.7e-10	48.28	WAT1-related protein At5g64700 OS=Arabidopsis thaliana OX=3702 GN=At5g64700 PE=2...	[more]
Q6NMB7	2.6e-05	48.94	WAT1-related protein At1g43650 OS=Arabidopsis thaliana OX=3702 GN=At1g43650 PE=2...	[more]
Q9ZUS1	7.5e-05	36.21	WAT1-related protein At2g37460 OS=Arabidopsis thaliana OX=3702 GN=At2g37460 PE=2...	[more]
F4I5D5	2.8e-04	34.92	WAT1-related protein At1g70260 OS=Arabidopsis thaliana OX=3702 GN=At1g70260 PE=2...	[more]
Q9SUD5	2.8e-04	40.43	WAT1-related protein At4g28040 OS=Arabidopsis thaliana OX=3702 GN=At4g28040 PE=2...	[more]

BLAST of Cmc08g0218921 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3B338	4.5e-29	100.00	WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103485253 PE=3 SV=1	[more]
A0A5A7SJQ3	5.4e-22	100.00	WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold253...	[more]
A0A0A0KFR8	5.4e-22	100.00	WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_6G484610 PE=3 SV=1	[more]
A0A5A7UFK5	2.0e-21	96.55	WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold102...	[more]
A0A5A7T859	7.7e-21	98.28	WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold767...	[more]

BLAST of Cmc08g0218921 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G64700.1	5.5e-11	48.28	nodulin MtN21 /EamA-like transporter family protein	[more]
AT1G43650.1	1.8e-06	48.94	nodulin MtN21 /EamA-like transporter family protein	[more]
AT1G43650.2	1.8e-06	48.94	nodulin MtN21 /EamA-like transporter family protein	[more]
AT2G37460.1	5.3e-06	36.21	nodulin MtN21 /EamA-like transporter family protein	[more]
AT1G70260.1	2.0e-05	34.92	nodulin MtN21 /EamA-like transporter family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR030184	WAT1-related protein	PANTHER	PTHR31218	WAT1-RELATED PROTEIN	coord: 35..86
None	No IPR available	PANTHER	PTHR31218:SF166	WAT1-RELATED PROTEIN	coord: 35..86

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cmc08g0218921.1	Cmc08g0218921.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0022857	transmembrane transporter activity