Cmc08g0217751 (gene) Melon (Charmono) v1.1

Overview
NameCmc08g0217751
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionAmmonium transporter
LocationCMiso1.1chr08: 6351010 .. 6352618 (-)
RNA-Seq ExpressionCmc08g0217751
SyntenyCmc08g0217751
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTTCCTGTAAAATTCAAGACTTCAGCTTGCTGCCGCCGGGGTTCGTTGATCCAGAACAAACATCTCCCATTTGGAACAACAAGGCTGACAATGCATGGCAGCTCACAGCAGCCACCATGGTCGGTCTACAAACCGTTCCCGGGCTGGTCATTCTCTATGGCAGCATGGTGAAGAAAAAATGGGCTGTCAACTCTGCCTTCATGGCCCTCTATGGCTTTGCAGCAGCGCTTGTCTGTTGGGTTTTGTGGGCACATCGTATGGCATTCGGCAAACGTTTGTTCGGGATCGTTGGTAAGCCCGGCCTAGCTCTGTCTGAGACCTACCTTCTTTCACAGTCTACACAAGGCTGTTTTCCGATGGCTGACTACGTGTTTTTCCAGTTTGCTTTCGCTGCCATTACTGTAGTGTTGCTTGCCGGGTCCTTGCTGGGAAGGATGAATTTTGGAGCATGGATGCTTTTTGTTCCAATGTGGCTTACTCTTTGTTATACTGTGGGGGCATTTTGTATATGGGGAGATGGGTTTATTCAGAAGTACCTAATTGACTATGCCGGCGGCTACGTCATTCATCTTTCTTCTGGGGTTGCTGGATTCACTGCTGCTTACTGGGTAAGTAACCTGCAAAACAAGTTTTTACTTTCTCCATATCTTTGATAGTAACGACAGCGACTGATTTGGTGACCATGCCCGCAGGTCGGGCCACGACACTCTCATGACAGGCAGAACTTCCCACCAAACAACATACTGCACATGGTTGGAGGTGCAGGGTTTCTTTGGCTGGGTTGGACTGGATTCAATGGAGGAGCTCCATTTGCAGCGAGTCGTATTACATCACTGGCCATTTTGAACACACATGTTTGCACCGCAACAAGTCTCTTGATCTGGGTTTCAATGGACATCATAGTATACAAGAAAAGCTCTGTGATTGGTGCAGTTCAAGGGATCATCACTGGCCTTGTTTGCATCACTCCGGGGGCAGGTTAGTCCTTTCAAAAAGAAAGAAACAAAAACATAACTGCTAATTTTACCTTACCCGAAGAATCCAAATGAATCCATGGTCTATGGTTTTACATGAATAATAGGGCTTGTGGACACTATCGCAGCCGTGGTGATGGGTGTTCTTGCTGGTTTGGTTCCATGGTACACCATGATGGTGCTGCATAGGAGGTTGGCATTCCTTCAGTGTGTTGACGATACTCTGGGCGTCTTCCACACACATGCTGTAGCTGGTTCCATGGGGGGACTCCTCACAGGCTTCTTTGCCAAACCCAGCCTTCTGAAAATGTTGTATCCAGATTCCCTATACGGACCAGGCTTCCTGTATGCCATCTCTGATAAAAAGCTCAAAGACGGCCTCAAACAAATCGGCATCCAGTTCGCAGGAGCAGTGTTTATTACCCTCTGGAATGCCATATTTACCAGCATTATATGTTACCTGATTAGCCATATTGTTAACTTAAGGATGAAAGAAGAGGACCTGGAGATTGGTGATGATGCAGCTCATGGAGAAGAACCCTACGCGTTGTGGGGTGATGGTGAAAAGATGCCATCTTCATTTCGTCTTCCACACACACCTAGGTTTCCTTCATTATGCAGGTGGGATTAG

mRNA sequence

ATGAGTTCCTGTAAAATTCAAGACTTCAGCTTGCTGCCGCCGGGGTTCGTTGATCCAGAACAAACATCTCCCATTTGGAACAACAAGGCTGACAATGCATGGCAGCTCACAGCAGCCACCATGGTCGGTCTACAAACCGTTCCCGGGCTGGTCATTCTCTATGGCAGCATGGTGAAGAAAAAATGGGCTGTCAACTCTGCCTTCATGGCCCTCTATGGCTTTGCAGCAGCGCTTGTCTGTTGGGTTTTGTGGGCACATCGTATGGCATTCGGCAAACGTTTGTTCGGGATCGTTGGTAAGCCCGGCCTAGCTCTGTCTGAGACCTACCTTCTTTCACAGTCTACACAAGGCTGTTTTCCGATGGCTGACTACGTGTTTTTCCAGTTTGCTTTCGCTGCCATTACTGTAGTGTTGCTTGCCGGGTCCTTGCTGGGAAGGATGAATTTTGGAGCATGGATGCTTTTTGTTCCAATGTGGCTTACTCTTTGTTATACTGTGGGGGCATTTTGTATATGGGGAGATGGGTTTATTCAGAAGTACCTAATTGACTATGCCGGCGGCTACGTCATTCATCTTTCTTCTGGGGTTGCTGGATTCACTGCTGCTTACTGGGTCGGGCCACGACACTCTCATGACAGGCAGAACTTCCCACCAAACAACATACTGCACATGGTTGGAGGTGCAGGGTTTCTTTGGCTGGGTTGGACTGGATTCAATGGAGGAGCTCCATTTGCAGCGAGTCGTATTACATCACTGGCCATTTTGAACACACATGTTTGCACCGCAACAAGTCTCTTGATCTGGGTTTCAATGGACATCATAGTATACAAGAAAAGCTCTGTGATTGGTGCAGTTCAAGGGATCATCACTGGCCTTGTTTGCATCACTCCGGGGGCAGGGCTTGTGGACACTATCGCAGCCGTGGTGATGGGTGTTCTTGCTGGTTTGGTTCCATGGTACACCATGATGGTGCTGCATAGGAGGTTGGCATTCCTTCAGTGTGTTGACGATACTCTGGGCGTCTTCCACACACATGCTGTAGCTGGTTCCATGGGGGGACTCCTCACAGGCTTCTTTGCCAAACCCAGCCTTCTGAAAATGTTGTATCCAGATTCCCTATACGGACCAGGCTTCCTGTATGCCATCTCTGATAAAAAGCTCAAAGACGGCCTCAAACAAATCGGCATCCAGTTCGCAGGAGCAGTGTTTATTACCCTCTGGAATGCCATATTTACCAGCATTATATGTTACCTGATTAGCCATATTGTTAACTTAAGGATGAAAGAAGAGGACCTGGAGATTGGTGATGATGCAGCTCATGGAGAAGAACCCTACGCGTTGTGGGGTGATGGTGAAAAGATGCCATCTTCATTTCGTCTTCCACACACACCTAGGTTTCCTTCATTATGCAGGTGGGATTAG

Coding sequence (CDS)

ATGAGTTCCTGTAAAATTCAAGACTTCAGCTTGCTGCCGCCGGGGTTCGTTGATCCAGAACAAACATCTCCCATTTGGAACAACAAGGCTGACAATGCATGGCAGCTCACAGCAGCCACCATGGTCGGTCTACAAACCGTTCCCGGGCTGGTCATTCTCTATGGCAGCATGGTGAAGAAAAAATGGGCTGTCAACTCTGCCTTCATGGCCCTCTATGGCTTTGCAGCAGCGCTTGTCTGTTGGGTTTTGTGGGCACATCGTATGGCATTCGGCAAACGTTTGTTCGGGATCGTTGGTAAGCCCGGCCTAGCTCTGTCTGAGACCTACCTTCTTTCACAGTCTACACAAGGCTGTTTTCCGATGGCTGACTACGTGTTTTTCCAGTTTGCTTTCGCTGCCATTACTGTAGTGTTGCTTGCCGGGTCCTTGCTGGGAAGGATGAATTTTGGAGCATGGATGCTTTTTGTTCCAATGTGGCTTACTCTTTGTTATACTGTGGGGGCATTTTGTATATGGGGAGATGGGTTTATTCAGAAGTACCTAATTGACTATGCCGGCGGCTACGTCATTCATCTTTCTTCTGGGGTTGCTGGATTCACTGCTGCTTACTGGGTCGGGCCACGACACTCTCATGACAGGCAGAACTTCCCACCAAACAACATACTGCACATGGTTGGAGGTGCAGGGTTTCTTTGGCTGGGTTGGACTGGATTCAATGGAGGAGCTCCATTTGCAGCGAGTCGTATTACATCACTGGCCATTTTGAACACACATGTTTGCACCGCAACAAGTCTCTTGATCTGGGTTTCAATGGACATCATAGTATACAAGAAAAGCTCTGTGATTGGTGCAGTTCAAGGGATCATCACTGGCCTTGTTTGCATCACTCCGGGGGCAGGGCTTGTGGACACTATCGCAGCCGTGGTGATGGGTGTTCTTGCTGGTTTGGTTCCATGGTACACCATGATGGTGCTGCATAGGAGGTTGGCATTCCTTCAGTGTGTTGACGATACTCTGGGCGTCTTCCACACACATGCTGTAGCTGGTTCCATGGGGGGACTCCTCACAGGCTTCTTTGCCAAACCCAGCCTTCTGAAAATGTTGTATCCAGATTCCCTATACGGACCAGGCTTCCTGTATGCCATCTCTGATAAAAAGCTCAAAGACGGCCTCAAACAAATCGGCATCCAGTTCGCAGGAGCAGTGTTTATTACCCTCTGGAATGCCATATTTACCAGCATTATATGTTACCTGATTAGCCATATTGTTAACTTAAGGATGAAAGAAGAGGACCTGGAGATTGGTGATGATGCAGCTCATGGAGAAGAACCCTACGCGTTGTGGGGTGATGGTGAAAAGATGCCATCTTCATTTCGTCTTCCACACACACCTAGGTTTCCTTCATTATGCAGGTGGGATTAG

Protein sequence

MSSCKIQDFSLLPPGFVDPEQTSPIWNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKKKWAVNSAFMALYGFAAALVCWVLWAHRMAFGKRLFGIVGKPGLALSETYLLSQSTQGCFPMADYVFFQFAFAAITVVLLAGSLLGRMNFGAWMLFVPMWLTLCYTVGAFCIWGDGFIQKYLIDYAGGYVIHLSSGVAGFTAAYWVGPRHSHDRQNFPPNNILHMVGGAGFLWLGWTGFNGGAPFAASRITSLAILNTHVCTATSLLIWVSMDIIVYKKSSVIGAVQGIITGLVCITPGAGLVDTIAAVVMGVLAGLVPWYTMMVLHRRLAFLQCVDDTLGVFHTHAVAGSMGGLLTGFFAKPSLLKMLYPDSLYGPGFLYAISDKKLKDGLKQIGIQFAGAVFITLWNAIFTSIICYLISHIVNLRMKEEDLEIGDDAAHGEEPYALWGDGEKMPSSFRLPHTPRFPSLCRWD
Homology
BLAST of Cmc08g0217751 vs. NCBI nr
Match: XP_008440880.1 (PREDICTED: LOW QUALITY PROTEIN: ammonium transporter 3 member 2-like [Cucumis melo])

HSP 1 Score: 952.6 bits (2461), Expect = 1.3e-273
Identity = 473/501 (94.41%), Postives = 473/501 (94.41%), Query Frame = 0

Query: 1   MSSCKIQDFSLLPPGFVDPEQTSPIWNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKK 60
           MSSCKIQDFSLLPPGFVDPEQTSPIWNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKK
Sbjct: 1   MSSCKIQDFSLLPPGFVDPEQTSPIWNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKK 60

Query: 61  KWAVNSAFMALYGFAAALVCWVLWAHRMAFGKRLFGIVGKPGLALSETYLLSQSTQGCFP 120
           KWAVNSAFMALYGFAAALVCWVLWAHRMAFGKRLFGIVGKPGLALSETYLLSQSTQGCFP
Sbjct: 61  KWAVNSAFMALYGFAAALVCWVLWAHRMAFGKRLFGIVGKPGLALSETYLLSQSTQGCFP 120

Query: 121 MADYVFFQFAFAAITVVLLAGSLLGRMNFGAWMLFVPMWLTLCYTVGAFCIWGDGFIQKY 180
           MADYVFFQFAFAAITVVLLAGSLLGRMNFGAWMLFVPMWLTLCYTVGAFCIWGDGFIQKY
Sbjct: 121 MADYVFFQFAFAAITVVLLAGSLLGRMNFGAWMLFVPMWLTLCYTVGAFCIWGDGFIQKY 180

Query: 181 LIDYAGGYVIHLSSGVAGFTAAYW----------------------------VGPRHSHD 240
           LIDYAGGYVIHLSSGVAGFTAAYW                            VGPRHSHD
Sbjct: 181 LIDYAGGYVIHLSSGVAGFTAAYWVSNLQNKFLLSPYLXXXRQRLIWXPCPQVGPRHSHD 240

Query: 241 RQNFPPNNILHMVGGAGFLWLGWTGFNGGAPFAASRITSLAILNTHVCTATSLLIWVSMD 300
           RQNFPPNNILHMVGGAGFLWLGWTGFNGGAPFAASRITSLAILNTHVCTATSLLIWVSMD
Sbjct: 241 RQNFPPNNILHMVGGAGFLWLGWTGFNGGAPFAASRITSLAILNTHVCTATSLLIWVSMD 300

Query: 301 IIVYKKSSVIGAVQGIITGLVCITPGAGLVDTIAAVVMGVLAGLVPWYTMMVLHRRLAFL 360
           IIVYKKSSVIGAVQGIITGLVCITPGAGLVDTIAAVVMGVLAGLVPWYTMMVLHRRLAFL
Sbjct: 301 IIVYKKSSVIGAVQGIITGLVCITPGAGLVDTIAAVVMGVLAGLVPWYTMMVLHRRLAFL 360

Query: 361 QCVDDTLGVFHTHAVAGSMGGLLTGFFAKPSLLKMLYPDSLYGPGFLYAISDKKLKDGLK 420
           QCVDDTLGVFHTHAVAGSMGGLLTGFFAKPSLLKMLYPDSLYGPGFLYAISDKKLKDGLK
Sbjct: 361 QCVDDTLGVFHTHAVAGSMGGLLTGFFAKPSLLKMLYPDSLYGPGFLYAISDKKLKDGLK 420

Query: 421 QIGIQFAGAVFITLWNAIFTSIICYLISHIVNLRMKEEDLEIGDDAAHGEEPYALWGDGE 474
           QIGIQFAGAVFITLWNAIFTSIICYLISHIVNLRMKEEDLEIGDDAAHGEEPYALWGDGE
Sbjct: 421 QIGIQFAGAVFITLWNAIFTSIICYLISHIVNLRMKEEDLEIGDDAAHGEEPYALWGDGE 480

BLAST of Cmc08g0217751 vs. NCBI nr
Match: XP_004135012.1 (ammonium transporter 3 member 3 [Cucumis sativus] >KAE8647622.1 hypothetical protein Csa_002936 [Cucumis sativus])

HSP 1 Score: 950.7 bits (2456), Expect = 4.9e-273
Identity = 463/473 (97.89%), Postives = 467/473 (98.73%), Query Frame = 0

Query: 1   MSSCKIQDFSLLPPGFVDPEQTSPIWNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKK 60
           MSSC IQDFSLL PGFVDPEQTSP WNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKK
Sbjct: 1   MSSCPIQDFSLLQPGFVDPEQTSPFWNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKK 60

Query: 61  KWAVNSAFMALYGFAAALVCWVLWAHRMAFGKRLFGIVGKPGLALSETYLLSQSTQGCFP 120
           KWAVNSAFMALYGFAA LVCWVLWAHRMAFGKRLFGIVGKPGLALSE+YLLS+STQGCFP
Sbjct: 61  KWAVNSAFMALYGFAAVLVCWVLWAHRMAFGKRLFGIVGKPGLALSESYLLSKSTQGCFP 120

Query: 121 MADYVFFQFAFAAITVVLLAGSLLGRMNFGAWMLFVPMWLTLCYTVGAFCIWGDGFIQKY 180
           MADYVFFQFAFAAITVVLLAGSLLGRMNFGAWMLFVPMWLTLCYTVGAFCIWGDGFIQKY
Sbjct: 121 MADYVFFQFAFAAITVVLLAGSLLGRMNFGAWMLFVPMWLTLCYTVGAFCIWGDGFIQKY 180

Query: 181 LIDYAGGYVIHLSSGVAGFTAAYWVGPRHSHDRQNFPPNNILHMVGGAGFLWLGWTGFNG 240
           LIDYAGGYVIHLSSGVAGFTAAYWVGPRHSHDRQNFPPNNILHMVGGAGFLWLGWTGFNG
Sbjct: 181 LIDYAGGYVIHLSSGVAGFTAAYWVGPRHSHDRQNFPPNNILHMVGGAGFLWLGWTGFNG 240

Query: 241 GAPFAASRITSLAILNTHVCTATSLLIWVSMDIIVYKKSSVIGAVQGIITGLVCITPGAG 300
           GAPFAA RITSLAILNTHVCTATSLLIWVSMDIIVYKKSSVIGAVQGIITGLVCITPGAG
Sbjct: 241 GAPFAAGRITSLAILNTHVCTATSLLIWVSMDIIVYKKSSVIGAVQGIITGLVCITPGAG 300

Query: 301 LVDTIAAVVMGVLAGLVPWYTMMVLHRRLAFLQCVDDTLGVFHTHAVAGSMGGLLTGFFA 360
           LVDTIAAVVMGVLAGLVPWYTMMVLHRRLAFLQCVDDTLGVFHTHAVAGSMGGLLTGFFA
Sbjct: 301 LVDTIAAVVMGVLAGLVPWYTMMVLHRRLAFLQCVDDTLGVFHTHAVAGSMGGLLTGFFA 360

Query: 361 KPSLLKMLYPDSLYGPGFLYAISDKKLKDGLKQIGIQFAGAVFITLWNAIFTSIICYLIS 420
           KPSLL+M+YPDSLYGPGFLYAISDKKLKDGLKQIGIQFAGAVFITLWNAIFTSIICYLIS
Sbjct: 361 KPSLLRMMYPDSLYGPGFLYAISDKKLKDGLKQIGIQFAGAVFITLWNAIFTSIICYLIS 420

Query: 421 HIVNLRMKEEDLEIGDDAAHGEEPYALWGDGEKMPSSFRLPHTPRFPSLCRWD 474
           HIVNLRM EEDLEIGDDAAHGEEPYALWGDGEKMPSSFRLPHTPRFPSLCRWD
Sbjct: 421 HIVNLRMNEEDLEIGDDAAHGEEPYALWGDGEKMPSSFRLPHTPRFPSLCRWD 473

BLAST of Cmc08g0217751 vs. NCBI nr
Match: KAA0025632.1 (ammonium transporter 3 member 3-like [Cucumis melo var. makuwa] >TYK12505.1 ammonium transporter 3 member 3-like [Cucumis melo var. makuwa])

HSP 1 Score: 944.9 bits (2441), Expect = 2.7e-271
Identity = 464/464 (100.00%), Postives = 464/464 (100.00%), Query Frame = 0

Query: 1   MSSCKIQDFSLLPPGFVDPEQTSPIWNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKK 60
           MSSCKIQDFSLLPPGFVDPEQTSPIWNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKK
Sbjct: 1   MSSCKIQDFSLLPPGFVDPEQTSPIWNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKK 60

Query: 61  KWAVNSAFMALYGFAAALVCWVLWAHRMAFGKRLFGIVGKPGLALSETYLLSQSTQGCFP 120
           KWAVNSAFMALYGFAAALVCWVLWAHRMAFGKRLFGIVGKPGLALSETYLLSQSTQGCFP
Sbjct: 61  KWAVNSAFMALYGFAAALVCWVLWAHRMAFGKRLFGIVGKPGLALSETYLLSQSTQGCFP 120

Query: 121 MADYVFFQFAFAAITVVLLAGSLLGRMNFGAWMLFVPMWLTLCYTVGAFCIWGDGFIQKY 180
           MADYVFFQFAFAAITVVLLAGSLLGRMNFGAWMLFVPMWLTLCYTVGAFCIWGDGFIQKY
Sbjct: 121 MADYVFFQFAFAAITVVLLAGSLLGRMNFGAWMLFVPMWLTLCYTVGAFCIWGDGFIQKY 180

Query: 181 LIDYAGGYVIHLSSGVAGFTAAYWVGPRHSHDRQNFPPNNILHMVGGAGFLWLGWTGFNG 240
           LIDYAGGYVIHLSSGVAGFTAAYWVGPRHSHDRQNFPPNNILHMVGGAGFLWLGWTGFNG
Sbjct: 181 LIDYAGGYVIHLSSGVAGFTAAYWVGPRHSHDRQNFPPNNILHMVGGAGFLWLGWTGFNG 240

Query: 241 GAPFAASRITSLAILNTHVCTATSLLIWVSMDIIVYKKSSVIGAVQGIITGLVCITPGAG 300
           GAPFAASRITSLAILNTHVCTATSLLIWVSMDIIVYKKSSVIGAVQGIITGLVCITPGAG
Sbjct: 241 GAPFAASRITSLAILNTHVCTATSLLIWVSMDIIVYKKSSVIGAVQGIITGLVCITPGAG 300

Query: 301 LVDTIAAVVMGVLAGLVPWYTMMVLHRRLAFLQCVDDTLGVFHTHAVAGSMGGLLTGFFA 360
           LVDTIAAVVMGVLAGLVPWYTMMVLHRRLAFLQCVDDTLGVFHTHAVAGSMGGLLTGFFA
Sbjct: 301 LVDTIAAVVMGVLAGLVPWYTMMVLHRRLAFLQCVDDTLGVFHTHAVAGSMGGLLTGFFA 360

Query: 361 KPSLLKMLYPDSLYGPGFLYAISDKKLKDGLKQIGIQFAGAVFITLWNAIFTSIICYLIS 420
           KPSLLKMLYPDSLYGPGFLYAISDKKLKDGLKQIGIQFAGAVFITLWNAIFTSIICYLIS
Sbjct: 361 KPSLLKMLYPDSLYGPGFLYAISDKKLKDGLKQIGIQFAGAVFITLWNAIFTSIICYLIS 420

Query: 421 HIVNLRMKEEDLEIGDDAAHGEEPYALWGDGEKMPSSFRLPHTP 465
           HIVNLRMKEEDLEIGDDAAHGEEPYALWGDGEKMPSSFRLPHTP
Sbjct: 421 HIVNLRMKEEDLEIGDDAAHGEEPYALWGDGEKMPSSFRLPHTP 464

BLAST of Cmc08g0217751 vs. NCBI nr
Match: XP_038881912.1 (ammonium transporter 2 member 3-like [Benincasa hispida])

HSP 1 Score: 916.0 bits (2366), Expect = 1.3e-262
Identity = 447/473 (94.50%), Postives = 457/473 (96.62%), Query Frame = 0

Query: 1   MSSCKIQDFSLLPPGFVDPEQTSPIWNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKK 60
           MSSC +Q F LL  GFVDPEQ +P WNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKK
Sbjct: 1   MSSCTLQGFGLL-LGFVDPEQATPDWNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKK 60

Query: 61  KWAVNSAFMALYGFAAALVCWVLWAHRMAFGKRLFGIVGKPGLALSETYLLSQSTQGCFP 120
           KWAVNSAFMALYGFAA LVCWVLWAHRMAFG RLF +VGKPG+ALSE+YLL QSTQGCFP
Sbjct: 61  KWAVNSAFMALYGFAAVLVCWVLWAHRMAFGDRLFWLVGKPGIALSESYLLKQSTQGCFP 120

Query: 121 MADYVFFQFAFAAITVVLLAGSLLGRMNFGAWMLFVPMWLTLCYTVGAFCIWGDGFIQKY 180
           MADYVFFQFAFAAITVVLLAGSLLGRMNFGAWMLFVPMWLT CYTVGAFCIWG+GFIQKY
Sbjct: 121 MADYVFFQFAFAAITVVLLAGSLLGRMNFGAWMLFVPMWLTFCYTVGAFCIWGNGFIQKY 180

Query: 181 LIDYAGGYVIHLSSGVAGFTAAYWVGPRHSHDRQNFPPNNILHMVGGAGFLWLGWTGFNG 240
           LIDYAGGYVIHLSSGVAGFTAAYWVGPRHS DRQNFPPNNILHMVGGAGFLW+GWTGFNG
Sbjct: 181 LIDYAGGYVIHLSSGVAGFTAAYWVGPRHSRDRQNFPPNNILHMVGGAGFLWMGWTGFNG 240

Query: 241 GAPFAASRITSLAILNTHVCTATSLLIWVSMDIIVYKKSSVIGAVQGIITGLVCITPGAG 300
           GAPFAA RITSLAILNTHVCTATSLLIWVSMDIIVYKKSSVIGAVQGIITGLVCITPGAG
Sbjct: 241 GAPFAAGRITSLAILNTHVCTATSLLIWVSMDIIVYKKSSVIGAVQGIITGLVCITPGAG 300

Query: 301 LVDTIAAVVMGVLAGLVPWYTMMVLHRRLAFLQCVDDTLGVFHTHAVAGSMGGLLTGFFA 360
           LVDT+AAVVMGVLAGLVPWYTMMVLHRRLAFLQCVDDTLGVFHTHAVAGSMGGLLTGFFA
Sbjct: 301 LVDTVAAVVMGVLAGLVPWYTMMVLHRRLAFLQCVDDTLGVFHTHAVAGSMGGLLTGFFA 360

Query: 361 KPSLLKMLYPDSLYGPGFLYAISDKKLKDGLKQIGIQFAGAVFITLWNAIFTSIICYLIS 420
           KPSLL+MLYPDSLY PGFLYAISD+KLKDGLKQIGIQ AGAVFITLWNAIFTSIICYLIS
Sbjct: 361 KPSLLRMLYPDSLYSPGFLYAISDRKLKDGLKQIGIQLAGAVFITLWNAIFTSIICYLIS 420

Query: 421 HIVNLRMKEEDLEIGDDAAHGEEPYALWGDGEKMPSSFRLPHTPRFPSLCRWD 474
           HIVNLRMKEEDLEIGDDAAHGEEPYALWGDGEKMPSSFRLPHTPRFPSLCRWD
Sbjct: 421 HIVNLRMKEEDLEIGDDAAHGEEPYALWGDGEKMPSSFRLPHTPRFPSLCRWD 472

BLAST of Cmc08g0217751 vs. NCBI nr
Match: KAG6603880.1 (Ammonium transporter 3 member 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034058.1 Ammonium transporter 3 member 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 894.0 bits (2309), Expect = 5.4e-256
Identity = 436/473 (92.18%), Postives = 452/473 (95.56%), Query Frame = 0

Query: 1   MSSCKIQDFSLLPPGFVDPEQTSPIWNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKK 60
           MSSC +QD   L PG    +Q+ P WNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKK
Sbjct: 1   MSSCMLQDRG-LRPGLAAADQSIPYWNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKK 60

Query: 61  KWAVNSAFMALYGFAAALVCWVLWAHRMAFGKRLFGIVGKPGLALSETYLLSQSTQGCFP 120
           KWAVNSAFMALYGFA+ LVCWVLWAHRMAFG RLF ++G PG ALSE YLLS STQGCFP
Sbjct: 61  KWAVNSAFMALYGFASVLVCWVLWAHRMAFGDRLFWLIGVPGAALSEGYLLSHSTQGCFP 120

Query: 121 MADYVFFQFAFAAITVVLLAGSLLGRMNFGAWMLFVPMWLTLCYTVGAFCIWGDGFIQKY 180
           MADYVFFQFAFAAITVVLLAGSLLGRMNFGAWMLFVPMWLT+CYTVGAFCIWG+GFIQKY
Sbjct: 121 MADYVFFQFAFAAITVVLLAGSLLGRMNFGAWMLFVPMWLTVCYTVGAFCIWGNGFIQKY 180

Query: 181 LIDYAGGYVIHLSSGVAGFTAAYWVGPRHSHDRQNFPPNNILHMVGGAGFLWLGWTGFNG 240
           LIDYAGGYVIHLSSGVAGFTAAYWVGPRHS DRQ+FPPNNILHMV GAGFLWLGWTGFNG
Sbjct: 181 LIDYAGGYVIHLSSGVAGFTAAYWVGPRHSRDRQSFPPNNILHMVFGAGFLWLGWTGFNG 240

Query: 241 GAPFAASRITSLAILNTHVCTATSLLIWVSMDIIVYKKSSVIGAVQGIITGLVCITPGAG 300
           GAPFAA RITSLAILNTH+CTATSLLIWVSMDIIVYKKSSVIGAVQGIITGLVCITPGAG
Sbjct: 241 GAPFAAGRITSLAILNTHICTATSLLIWVSMDIIVYKKSSVIGAVQGIITGLVCITPGAG 300

Query: 301 LVDTIAAVVMGVLAGLVPWYTMMVLHRRLAFLQCVDDTLGVFHTHAVAGSMGGLLTGFFA 360
           LVD++AAVVMGVLAGLVPWYTMMVLHR+LAFLQCVDDTLGVFHTHAVAGSMGGLLTGFFA
Sbjct: 301 LVDSVAAVVMGVLAGLVPWYTMMVLHRKLAFLQCVDDTLGVFHTHAVAGSMGGLLTGFFA 360

Query: 361 KPSLLKMLYPDSLYGPGFLYAISDKKLKDGLKQIGIQFAGAVFITLWNAIFTSIICYLIS 420
           KPSLLKM+YPDSLYGPGFLYAIS++KLKDGLKQIGIQFAGAVFITLWNAIFTSIICYLIS
Sbjct: 361 KPSLLKMMYPDSLYGPGFLYAISNRKLKDGLKQIGIQFAGAVFITLWNAIFTSIICYLIS 420

Query: 421 HIVNLRMKEEDLEIGDDAAHGEEPYALWGDGEKMPSSFRLPHTPRFPSLCRWD 474
           HIVNLRMKEEDLEIGDDAAHGEEPYALWGDGEKMPSSFRLPHTPRFPSLCRWD
Sbjct: 421 HIVNLRMKEEDLEIGDDAAHGEEPYALWGDGEKMPSSFRLPHTPRFPSLCRWD 472

BLAST of Cmc08g0217751 vs. ExPASy Swiss-Prot
Match: G7LAA8 (Ammonium transporter 2 member 3 OS=Medicago truncatula OX=3880 GN=AMT2-3 PE=2 SV=2)

HSP 1 Score: 667.2 bits (1720), Expect = 1.4e-190
Identity = 322/450 (71.56%), Postives = 374/450 (83.11%), Query Frame = 0

Query: 19  PEQTSPIWNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKKKWAVNSAFMALYGFAAAL 78
           P   SP WNNKADNAWQLTAAT+VGLQTVPGLVILYGSMVKKKWAVNSAFMALY FAA L
Sbjct: 18  PNDASPEWNNKADNAWQLTAATLVGLQTVPGLVILYGSMVKKKWAVNSAFMALYAFAAVL 77

Query: 79  VCWVLWAHRMAFGKRLFGIVGKPGLALSETYLLSQ-STQGCFPMADYVFFQFAFAAITVV 138
           VCWVLWAH MAFG +L   VGKP  ALS+ +LLS+ ST    PMAD+VF+QFAFAAIT+V
Sbjct: 78  VCWVLWAHHMAFGTKLLPFVGKPNFALSQKFLLSKASTNYYLPMADFVFYQFAFAAITLV 137

Query: 139 LLAGSLLGRMNFGAWMLFVPMWLTLCYTVGAFCIWGDGFIQKYLIDYAGGYVIHLSSGVA 198
           LL GSLLGRMNF AWMLFVP+WLTL YTVGAF IWG+GF++  +IDYAGG+VIHLSSGVA
Sbjct: 138 LLGGSLLGRMNFYAWMLFVPLWLTLSYTVGAFTIWGNGFLEGKIIDYAGGFVIHLSSGVA 197

Query: 199 GFTAAYWVGPRHSHDRQNFPPNNILHMVGGAGFLWLGWTGFNGGAPFAASRITSLAILNT 258
           GFTAAYWVGPR S+DRQNFPPNNI+HM+GGAGFLW+GWTGFNGGAPF    ITSLAI NT
Sbjct: 198 GFTAAYWVGPRTSNDRQNFPPNNIIHMLGGAGFLWMGWTGFNGGAPFQVGEITSLAIFNT 257

Query: 259 HVCTATSLLIWVSMDIIVYKKSSVIGAVQGIITGLVCITPGAGLVDTIAAVVMGVLAGLV 318
           H+CTATS+L+W+S+D+ VYKK S+IG+VQG++TGLVCITPGAGLVD  AA++MG L+G +
Sbjct: 258 HLCTATSILVWISLDMAVYKKGSLIGSVQGMMTGLVCITPGAGLVDPWAAILMGALSGSI 317

Query: 319 PWYTMMVLHRRLAFLQCVDDTLGVFHTHAVAGSMGGLLTGFFAKPSLLKMLYPDSLYGPG 378
           PWYTMMVLH++  F Q VDDTLGVFHTHAVAG +GG+L+G FAKP LL++LY    YG G
Sbjct: 318 PWYTMMVLHKKSPFFQSVDDTLGVFHTHAVAGILGGILSGVFAKPKLLRILY--GPYGSG 377

Query: 379 FLYAISDKKLKDGLKQIGIQFAGAVFITLWNAIFTSIICYLISHIVNLRMKEEDLEIGDD 438
            LY+  D  +  G+KQ+  Q  GAVFIT+WN + TS+IC L++  VNLRM+EEDLE+GDD
Sbjct: 378 LLYSYFDDNIGQGIKQMWYQLLGAVFITIWNVVITSLICILLNRFVNLRMQEEDLEVGDD 437

Query: 439 AAHGEEPYALWGDGEKMPSSFRLPHTPRFP 468
           AAHGEE Y LWGDGE+M    R   +P  P
Sbjct: 438 AAHGEEAYVLWGDGERMRLPLRRDISPIIP 465

BLAST of Cmc08g0217751 vs. ExPASy Swiss-Prot
Match: A0A072VHJ1 (Ammonium transporter 2 member 5 OS=Medicago truncatula OX=3880 GN=AMT2-5 PE=2 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 9.6e-155
Identity = 268/446 (60.09%), Postives = 330/446 (73.99%), Query Frame = 0

Query: 14  PGFVDPEQTSPIWNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKKKWAVNSAFMALYG 73
           P  + P  +SP W +KADNAWQL AAT+VG+Q+VPGL+ILYG  VKKKWAVNSAFM+LY 
Sbjct: 7   PSNLLPSPSSPEWLSKADNAWQLMAATLVGMQSVPGLIILYGGAVKKKWAVNSAFMSLYA 66

Query: 74  FAAALVCWVLWAHRMAFGKRLFGIVGKPGLALSETYLLSQSTQGCFPMADYVFFQFAFAA 133
           FA    CWV WA+RM+FG  LF   GKP LAL E YL  Q+  G FP A  ++FQ  FAA
Sbjct: 67  FACVFFCWVFWAYRMSFGDTLFPFWGKPALALDEKYLFKQAFLGAFPNATMIYFQCVFAA 126

Query: 134 ITVVLLAGSLLGRMNFGAWMLFVPMWLTLCYTVGAFCIWG-DGFIQKY-LIDYAGGYVIH 193
           IT++L+AG++LGRMNF AWM+FVP+WLT  YT  AF IW  +GF+ K  +IDY+GGYVIH
Sbjct: 127 ITLILIAGAVLGRMNFYAWMMFVPLWLTFSYTFTAFSIWSTNGFLAKMGIIDYSGGYVIH 186

Query: 194 LSSGVAGFTAAYWVGPRHSHDRQNFPPNNILHMVGGAGFLWLGWTGFNGGAPFAASRITS 253
           LSSGVAGFTAAYWVGPR + DR+ FPPNN+L M+ GAG LW+GWTGFNGG P++     S
Sbjct: 187 LSSGVAGFTAAYWVGPRLNKDRERFPPNNLLLMLAGAGLLWMGWTGFNGGDPYSVGLDAS 246

Query: 254 LAILNTHVCTATSLLIWVSMDIIVYKKSSVIGAVQGIITGLVCITPGAGLVDTIAAVVMG 313
           LA+LNTH CTATSLL WV +D+I ++K SVIGAVQG+ITGLVCITP AG+V   AA++MG
Sbjct: 247 LAVLNTHACTATSLLTWVFLDVIFFRKPSVIGAVQGMITGLVCITPAAGVVQGWAALIMG 306

Query: 314 VLAGLVPWYTMMVLHRRLAFLQCVDDTLGVFHTHAVAGSMGGLLTGFFAKPSLLKMLYPD 373
           + +G +PW+TMMV+H+R   LQ VDDT+ V HTHA+AGS+GG+LTG FA+P L  + Y  
Sbjct: 307 LFSGSIPWFTMMVIHKRSKLLQKVDDTMAVLHTHAIAGSLGGILTGLFAEPKLNSLFYGV 366

Query: 374 SLYGPGFLYAISDKKLKDGLKQIGIQFAGAVFITLWNAIFTSIICYLISHIVNLRMKEED 433
                G  Y I       G +QIGIQ  G +F+   N + TS+IC  I   V LRM EED
Sbjct: 367 YNKYVGLFYGIQMNMASTGRRQIGIQLLGILFVIFVNVVSTSLICLFIRLFVPLRMSEED 426

Query: 434 LEIGDDAAHGEEPYALWGDGEKMPSS 458
           +EIGD+AAHGEE YA+WG G+K  +S
Sbjct: 427 MEIGDEAAHGEEAYAIWGQGDKHENS 452

BLAST of Cmc08g0217751 vs. ExPASy Swiss-Prot
Match: Q84KJ6 (Ammonium transporter 3 member 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT3-1 PE=2 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 3.5e-149
Identity = 270/469 (57.57%), Postives = 333/469 (71.00%), Query Frame = 0

Query: 13  PPGFVDPEQTSPIWNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKKKWAVNSAFMALY 72
           P G   PE     W NK DNAWQ+ +AT+VG+Q+VPGLVILYGS+VKKKWAVNSAFMALY
Sbjct: 14  PSGMAVPE-----WLNKGDNAWQMISATLVGMQSVPGLVILYGSIVKKKWAVNSAFMALY 73

Query: 73  GFAAALVCWVLWAHRMAFGKRLFGIVGKPGLALSETYLLSQS------------------ 132
            FAA  +CWV W + M+FG +L    GK   AL +++LL+Q+                  
Sbjct: 74  AFAAVWLCWVTWGYNMSFGHKLLPFWGKARPALGQSFLLAQAVLPQTTQFYKGGGGADAV 133

Query: 133 -----TQGCFPMADYVFFQFAFAAITVVLLAGSLLGRMNFGAWMLFVPMWLTLCYTVGAF 192
                    +PMA  V+FQ  FAAIT++LLAGSLLGRMN  AWMLFVP+WLT  YTVGAF
Sbjct: 134 VETPWVNPLYPMATMVYFQCVFAAITLILLAGSLLGRMNIKAWMLFVPLWLTFSYTVGAF 193

Query: 193 CIWGDGFIQKY-LIDYAGGYVIHLSSGVAGFTAAYWVGPRHSHDRQNFPPNNILHMVGGA 252
            +WG GF+  + ++DY+GGYVIHLSSGVAGFTAAYWVGPR + DR+ FPPNN+L M+ GA
Sbjct: 194 SLWGGGFLFHWGVMDYSGGYVIHLSSGVAGFTAAYWVGPRSTKDRERFPPNNVLLMLTGA 253

Query: 253 GFLWLGWTGFNGGAPFAASRITSLAILNTHVCTATSLLIWVSMDIIVYKKSSVIGAVQGI 312
           G LW+GW GFNGG P++A+  +SLA+LNT++C ATSLL+W  +D+I +KK SVIGAVQG+
Sbjct: 254 GILWMGWAGFNGGDPYSANIDSSLAVLNTNICAATSLLVWTCLDVIFFKKPSVIGAVQGM 313

Query: 313 ITGLVCITPGAGLVDTIAAVVMGVLAGLVPWYTMMVLHRRLAFLQCVDDTLGVFHTHAVA 372
           ITGLVCITPGAGLV   AA+VMG+L+G +PW+TMMV+H+R   LQ VDDTLGVFHTHAVA
Sbjct: 314 ITGLVCITPGAGLVQGWAAIVMGILSGSIPWFTMMVVHKRSRLLQQVDDTLGVFHTHAVA 373

Query: 373 GSMGGLLTGFFAKPSLLKMLYPDSLYGPGFLYAISDKKLKDGLKQIGIQFAGAVFITLWN 432
           G +GG  TG FA+P L  +  P +     F         + G  Q   Q AGA+FI  WN
Sbjct: 374 GFLGGATTGLFAEPVLCSLFLPVTNSRGAFYPG------RGGGLQFVRQVAGALFIICWN 433

Query: 433 AIFTSIICYLISHIVNLRMKEEDLEIGDDAAHGEEPYALWGDGEKMPSS 458
            + TS++C  +  +V LRM EE+L IGDDA HGEE YALWGDGEK  S+
Sbjct: 434 VVVTSLVCLAVRAVVPLRMPEEELAIGDDAVHGEEAYALWGDGEKYDST 471

BLAST of Cmc08g0217751 vs. ExPASy Swiss-Prot
Match: Q69T29 (Ammonium transporter 3 member 3 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT3-3 PE=2 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 1.8e-148
Identity = 263/452 (58.19%), Postives = 337/452 (74.56%), Query Frame = 0

Query: 22  TSPIWNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKKKWAVNSAFMALYGFAAALVCW 81
           +SP W NK DNAWQ+T+AT+VGLQ++PGLVILYGS+VKKKWA+NSAFMALY FAA  +CW
Sbjct: 15  SSPDWLNKGDNAWQMTSATLVGLQSMPGLVILYGSIVKKKWAINSAFMALYAFAAVWICW 74

Query: 82  VLWAHRMAFGKRLFGIVGKPGLALSETYLLSQS------------------TQGCFPMAD 141
           V+WA+ M+FG RL    GK   AL +++L++QS                   +  +P+A 
Sbjct: 75  VVWAYNMSFGDRLLPFWGKARPALGQSFLVAQSELTATAIRYHNGSAEAPMLKPLYPVAT 134

Query: 142 YVFFQFAFAAITVVLLAGSLLGRMNFGAWMLFVPMWLTLCYTVGAFCIWGDGFIQKY-LI 201
            V+FQ  FA+IT+++LAGSLLGRMN  AWM FVP+W+T  YTV AF +WG GF+ ++ +I
Sbjct: 135 MVYFQCMFASITIIILAGSLLGRMNIKAWMAFVPLWITFSYTVCAFSLWGGGFLFQWGVI 194

Query: 202 DYAGGYVIHLSSGVAGFTAAYWVGPRHSHDRQNFPPNNILHMVGGAGFLWLGWTGFNGGA 261
           DY+GGYVIHLSSG+AG TAAYWVGPR + DR+ FPPNNIL ++ GAG LWLGWTGFNGG 
Sbjct: 195 DYSGGYVIHLSSGIAGLTAAYWVGPRSASDRERFPPNNILLVLAGAGLLWLGWTGFNGGD 254

Query: 262 PFAASRITSLAILNTHVCTATSLLIWVSMDIIVYKKSSVIGAVQGIITGLVCITPGAGLV 321
           P++A+  +S+A+LNTH+C +TSLL+W  +D+  + K SVIGAVQG+ITGLVCITPGAGLV
Sbjct: 255 PYSANIDSSMAVLNTHICASTSLLVWTILDVFFFGKPSVIGAVQGMITGLVCITPGAGLV 314

Query: 322 DTIAAVVMGVLAGLVPWYTMMVLHRRLAFLQCVDDTLGVFHTHAVAGSMGGLLTGFFAKP 381
              AA+VMG+L+G +PWYTMMVLH++ +F+Q +DDTLGVFHTHAVAG +GG  TG FA+P
Sbjct: 315 QGWAAIVMGILSGSIPWYTMMVLHKKWSFMQRIDDTLGVFHTHAVAGFLGGATTGLFAEP 374

Query: 382 SL--LKMLYPDSLYGPGFLYAISDKKLKDGLKQIGIQFAGAVFITLWNAIFTSIICYLIS 441
            L  L +  PDS    G  Y         G  Q G Q AGA+F+T WN + TSIIC +IS
Sbjct: 375 ILCSLFLSIPDS---KGAFYG------GPGGSQFGKQIAGALFVTAWNIVITSIICVIIS 434

Query: 442 HIVNLRMKEEDLEIGDDAAHGEEPYALWGDGE 453
            I+ LR+ +++L IGDDA HGEE YA+W +GE
Sbjct: 435 LILPLRIADQELLIGDDAVHGEEAYAIWAEGE 457

BLAST of Cmc08g0217751 vs. ExPASy Swiss-Prot
Match: Q851M9 (Ammonium transporter 3 member 2 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT3-2 PE=2 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 8.7e-148
Identity = 261/446 (58.52%), Postives = 326/446 (73.09%), Query Frame = 0

Query: 26  WNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKKKWAVNSAFMALYGFAAALVCWVLWA 85
           W NK DNAWQL AAT+VGLQ+VPGLV+LYG +VKKKWAVNSAFMALY FAA  +CWV WA
Sbjct: 23  WLNKGDNAWQLVAATVVGLQSVPGLVVLYGGVVKKKWAVNSAFMALYAFAAVWICWVTWA 82

Query: 86  HRMAFGKRLFGIVGKPGLALSETYLLSQ------------------STQGCFPMADYVFF 145
           + M+FG++L  I GK   AL +  L+ +                  + +  +PMA  V+F
Sbjct: 83  YNMSFGEKLLPIWGKARPALDQGLLVGRAALPATVHYRADGSVETAAVEPLYPMATVVYF 142

Query: 146 QFAFAAITVVLLAGSLLGRMNFGAWMLFVPMWLTLCYTVGAFCIWGDGFIQKY-LIDYAG 205
           Q  FAAIT++L+AGSLLGRM+F AWM+FVP+WLT  YTVGAF +WG GF+  + +IDY G
Sbjct: 143 QCVFAAITLILVAGSLLGRMSFLAWMIFVPLWLTFSYTVGAFSLWGGGFLFHWGVIDYCG 202

Query: 206 GYVIHLSSGVAGFTAAYWVGPRHSHDRQNFPPNNILHMVGGAGFLWLGWTGFNGGAPFAA 265
           GYVIH+S+G+AGFTAAYWVGPR   DR+ FPPNNIL  + GAG LW+GW GFNGG P+AA
Sbjct: 203 GYVIHVSAGIAGFTAAYWVGPRAQKDRERFPPNNILFTLTGAGLLWMGWAGFNGGGPYAA 262

Query: 266 SRITSLAILNTHVCTATSLLIWVSMDIIVYKKSSVIGAVQGIITGLVCITPGAGLVDTIA 325
           + + S+A+LNT++CTA SL++W  +D+I +KK SV+GAVQG+ITGLVCITP AG+V   A
Sbjct: 263 NSVASMAVLNTNICTAMSLIVWTCLDVIFFKKPSVVGAVQGMITGLVCITPAAGVVQGWA 322

Query: 326 AVVMGVLAGLVPWYTMMVLHRRLAFLQCVDDTLGVFHTHAVAGSMGGLLTGFFAKPSLLK 385
           A+VMGVLAG +PWYTMM+LH+R   LQ VDDTLGVFHTH VAG +GGLLTG FA+P+L  
Sbjct: 323 ALVMGVLAGSIPWYTMMILHKRSKILQRVDDTLGVFHTHGVAGLLGGLLTGLFAEPTLCN 382

Query: 386 MLYPDSLYGPGFLYAISDKKLKDGLKQIGIQFAGAVFITLWNAIFTSIICYLISHIVNLR 445
           +  P +    G  Y  +      G  Q G Q AG +F+  WN   TS+IC  I+ +V LR
Sbjct: 383 LFLPVA-DSRGAFYGGA------GGAQFGKQIAGGLFVVAWNVAVTSLICLAINLLVPLR 442

Query: 446 MKEEDLEIGDDAAHGEEPYALWGDGE 453
           M ++ LE+GDDA HGEE YALWGDGE
Sbjct: 443 MPDDKLEVGDDAVHGEEAYALWGDGE 461

BLAST of Cmc08g0217751 vs. ExPASy TrEMBL
Match: A0A1S3B266 (LOW QUALITY PROTEIN: ammonium transporter 3 member 2-like OS=Cucumis melo OX=3656 GN=LOC103485169 PE=4 SV=1)

HSP 1 Score: 952.6 bits (2461), Expect = 6.2e-274
Identity = 473/501 (94.41%), Postives = 473/501 (94.41%), Query Frame = 0

Query: 1   MSSCKIQDFSLLPPGFVDPEQTSPIWNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKK 60
           MSSCKIQDFSLLPPGFVDPEQTSPIWNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKK
Sbjct: 1   MSSCKIQDFSLLPPGFVDPEQTSPIWNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKK 60

Query: 61  KWAVNSAFMALYGFAAALVCWVLWAHRMAFGKRLFGIVGKPGLALSETYLLSQSTQGCFP 120
           KWAVNSAFMALYGFAAALVCWVLWAHRMAFGKRLFGIVGKPGLALSETYLLSQSTQGCFP
Sbjct: 61  KWAVNSAFMALYGFAAALVCWVLWAHRMAFGKRLFGIVGKPGLALSETYLLSQSTQGCFP 120

Query: 121 MADYVFFQFAFAAITVVLLAGSLLGRMNFGAWMLFVPMWLTLCYTVGAFCIWGDGFIQKY 180
           MADYVFFQFAFAAITVVLLAGSLLGRMNFGAWMLFVPMWLTLCYTVGAFCIWGDGFIQKY
Sbjct: 121 MADYVFFQFAFAAITVVLLAGSLLGRMNFGAWMLFVPMWLTLCYTVGAFCIWGDGFIQKY 180

Query: 181 LIDYAGGYVIHLSSGVAGFTAAYW----------------------------VGPRHSHD 240
           LIDYAGGYVIHLSSGVAGFTAAYW                            VGPRHSHD
Sbjct: 181 LIDYAGGYVIHLSSGVAGFTAAYWVSNLQNKFLLSPYLXXXRQRLIWXPCPQVGPRHSHD 240

Query: 241 RQNFPPNNILHMVGGAGFLWLGWTGFNGGAPFAASRITSLAILNTHVCTATSLLIWVSMD 300
           RQNFPPNNILHMVGGAGFLWLGWTGFNGGAPFAASRITSLAILNTHVCTATSLLIWVSMD
Sbjct: 241 RQNFPPNNILHMVGGAGFLWLGWTGFNGGAPFAASRITSLAILNTHVCTATSLLIWVSMD 300

Query: 301 IIVYKKSSVIGAVQGIITGLVCITPGAGLVDTIAAVVMGVLAGLVPWYTMMVLHRRLAFL 360
           IIVYKKSSVIGAVQGIITGLVCITPGAGLVDTIAAVVMGVLAGLVPWYTMMVLHRRLAFL
Sbjct: 301 IIVYKKSSVIGAVQGIITGLVCITPGAGLVDTIAAVVMGVLAGLVPWYTMMVLHRRLAFL 360

Query: 361 QCVDDTLGVFHTHAVAGSMGGLLTGFFAKPSLLKMLYPDSLYGPGFLYAISDKKLKDGLK 420
           QCVDDTLGVFHTHAVAGSMGGLLTGFFAKPSLLKMLYPDSLYGPGFLYAISDKKLKDGLK
Sbjct: 361 QCVDDTLGVFHTHAVAGSMGGLLTGFFAKPSLLKMLYPDSLYGPGFLYAISDKKLKDGLK 420

Query: 421 QIGIQFAGAVFITLWNAIFTSIICYLISHIVNLRMKEEDLEIGDDAAHGEEPYALWGDGE 474
           QIGIQFAGAVFITLWNAIFTSIICYLISHIVNLRMKEEDLEIGDDAAHGEEPYALWGDGE
Sbjct: 421 QIGIQFAGAVFITLWNAIFTSIICYLISHIVNLRMKEEDLEIGDDAAHGEEPYALWGDGE 480

BLAST of Cmc08g0217751 vs. ExPASy TrEMBL
Match: A0A5D3CLR1 (Ammonium transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00850 PE=3 SV=1)

HSP 1 Score: 944.9 bits (2441), Expect = 1.3e-271
Identity = 464/464 (100.00%), Postives = 464/464 (100.00%), Query Frame = 0

Query: 1   MSSCKIQDFSLLPPGFVDPEQTSPIWNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKK 60
           MSSCKIQDFSLLPPGFVDPEQTSPIWNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKK
Sbjct: 1   MSSCKIQDFSLLPPGFVDPEQTSPIWNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKK 60

Query: 61  KWAVNSAFMALYGFAAALVCWVLWAHRMAFGKRLFGIVGKPGLALSETYLLSQSTQGCFP 120
           KWAVNSAFMALYGFAAALVCWVLWAHRMAFGKRLFGIVGKPGLALSETYLLSQSTQGCFP
Sbjct: 61  KWAVNSAFMALYGFAAALVCWVLWAHRMAFGKRLFGIVGKPGLALSETYLLSQSTQGCFP 120

Query: 121 MADYVFFQFAFAAITVVLLAGSLLGRMNFGAWMLFVPMWLTLCYTVGAFCIWGDGFIQKY 180
           MADYVFFQFAFAAITVVLLAGSLLGRMNFGAWMLFVPMWLTLCYTVGAFCIWGDGFIQKY
Sbjct: 121 MADYVFFQFAFAAITVVLLAGSLLGRMNFGAWMLFVPMWLTLCYTVGAFCIWGDGFIQKY 180

Query: 181 LIDYAGGYVIHLSSGVAGFTAAYWVGPRHSHDRQNFPPNNILHMVGGAGFLWLGWTGFNG 240
           LIDYAGGYVIHLSSGVAGFTAAYWVGPRHSHDRQNFPPNNILHMVGGAGFLWLGWTGFNG
Sbjct: 181 LIDYAGGYVIHLSSGVAGFTAAYWVGPRHSHDRQNFPPNNILHMVGGAGFLWLGWTGFNG 240

Query: 241 GAPFAASRITSLAILNTHVCTATSLLIWVSMDIIVYKKSSVIGAVQGIITGLVCITPGAG 300
           GAPFAASRITSLAILNTHVCTATSLLIWVSMDIIVYKKSSVIGAVQGIITGLVCITPGAG
Sbjct: 241 GAPFAASRITSLAILNTHVCTATSLLIWVSMDIIVYKKSSVIGAVQGIITGLVCITPGAG 300

Query: 301 LVDTIAAVVMGVLAGLVPWYTMMVLHRRLAFLQCVDDTLGVFHTHAVAGSMGGLLTGFFA 360
           LVDTIAAVVMGVLAGLVPWYTMMVLHRRLAFLQCVDDTLGVFHTHAVAGSMGGLLTGFFA
Sbjct: 301 LVDTIAAVVMGVLAGLVPWYTMMVLHRRLAFLQCVDDTLGVFHTHAVAGSMGGLLTGFFA 360

Query: 361 KPSLLKMLYPDSLYGPGFLYAISDKKLKDGLKQIGIQFAGAVFITLWNAIFTSIICYLIS 420
           KPSLLKMLYPDSLYGPGFLYAISDKKLKDGLKQIGIQFAGAVFITLWNAIFTSIICYLIS
Sbjct: 361 KPSLLKMLYPDSLYGPGFLYAISDKKLKDGLKQIGIQFAGAVFITLWNAIFTSIICYLIS 420

Query: 421 HIVNLRMKEEDLEIGDDAAHGEEPYALWGDGEKMPSSFRLPHTP 465
           HIVNLRMKEEDLEIGDDAAHGEEPYALWGDGEKMPSSFRLPHTP
Sbjct: 421 HIVNLRMKEEDLEIGDDAAHGEEPYALWGDGEKMPSSFRLPHTP 464

BLAST of Cmc08g0217751 vs. ExPASy TrEMBL
Match: A0A0A0KHE1 (Ammonium transporter OS=Cucumis sativus OX=3659 GN=Csa_6G507360 PE=3 SV=1)

HSP 1 Score: 901.4 bits (2328), Expect = 1.6e-258
Identity = 442/452 (97.79%), Postives = 446/452 (98.67%), Query Frame = 0

Query: 1   MSSCKIQDFSLLPPGFVDPEQTSPIWNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKK 60
           MSSC IQDFSLL PGFVDPEQTSP WNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKK
Sbjct: 1   MSSCPIQDFSLLQPGFVDPEQTSPFWNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKK 60

Query: 61  KWAVNSAFMALYGFAAALVCWVLWAHRMAFGKRLFGIVGKPGLALSETYLLSQSTQGCFP 120
           KWAVNSAFMALYGFAA LVCWVLWAHRMAFGKRLFGIVGKPGLALSE+YLLS+STQGCFP
Sbjct: 61  KWAVNSAFMALYGFAAVLVCWVLWAHRMAFGKRLFGIVGKPGLALSESYLLSKSTQGCFP 120

Query: 121 MADYVFFQFAFAAITVVLLAGSLLGRMNFGAWMLFVPMWLTLCYTVGAFCIWGDGFIQKY 180
           MADYVFFQFAFAAITVVLLAGSLLGRMNFGAWMLFVPMWLTLCYTVGAFCIWGDGFIQKY
Sbjct: 121 MADYVFFQFAFAAITVVLLAGSLLGRMNFGAWMLFVPMWLTLCYTVGAFCIWGDGFIQKY 180

Query: 181 LIDYAGGYVIHLSSGVAGFTAAYWVGPRHSHDRQNFPPNNILHMVGGAGFLWLGWTGFNG 240
           LIDYAGGYVIHLSSGVAGFTAAYWVGPRHSHDRQNFPPNNILHMVGGAGFLWLGWTGFNG
Sbjct: 181 LIDYAGGYVIHLSSGVAGFTAAYWVGPRHSHDRQNFPPNNILHMVGGAGFLWLGWTGFNG 240

Query: 241 GAPFAASRITSLAILNTHVCTATSLLIWVSMDIIVYKKSSVIGAVQGIITGLVCITPGAG 300
           GAPFAA RITSLAILNTHVCTATSLLIWVSMDIIVYKKSSVIGAVQGIITGLVCITPGAG
Sbjct: 241 GAPFAAGRITSLAILNTHVCTATSLLIWVSMDIIVYKKSSVIGAVQGIITGLVCITPGAG 300

Query: 301 LVDTIAAVVMGVLAGLVPWYTMMVLHRRLAFLQCVDDTLGVFHTHAVAGSMGGLLTGFFA 360
           LVDTIAAVVMGVLAGLVPWYTMMVLHRRLAFLQCVDDTLGVFHTHAVAGSMGGLLTGFFA
Sbjct: 301 LVDTIAAVVMGVLAGLVPWYTMMVLHRRLAFLQCVDDTLGVFHTHAVAGSMGGLLTGFFA 360

Query: 361 KPSLLKMLYPDSLYGPGFLYAISDKKLKDGLKQIGIQFAGAVFITLWNAIFTSIICYLIS 420
           KPSLL+M+YPDSLYGPGFLYAISDKKLKDGLKQIGIQFAGAVFITLWNAIFTSIICYLIS
Sbjct: 361 KPSLLRMMYPDSLYGPGFLYAISDKKLKDGLKQIGIQFAGAVFITLWNAIFTSIICYLIS 420

Query: 421 HIVNLRMKEEDLEIGDDAAHGEEPYALWGDGE 453
           HIVNLRM EEDLEIGDDAAHGEEPYALWGDGE
Sbjct: 421 HIVNLRMNEEDLEIGDDAAHGEEPYALWGDGE 452

BLAST of Cmc08g0217751 vs. ExPASy TrEMBL
Match: A0A6J1BTX0 (Ammonium transporter OS=Momordica charantia OX=3673 GN=LOC111005728 PE=3 SV=1)

HSP 1 Score: 867.8 bits (2241), Expect = 2.0e-248
Identity = 421/454 (92.73%), Postives = 435/454 (95.81%), Query Frame = 0

Query: 20  EQTSPIWNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKKKWAVNSAFMALYGFAAALV 79
           +Q+ P WNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKKKWAVNSAFMALYGFAA LV
Sbjct: 16  DQSIPYWNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKKKWAVNSAFMALYGFAAVLV 75

Query: 80  CWVLWAHRMAFGKRLFGIVGKPGLALSETYLLSQSTQGCFPMADYVFFQFAFAAITVVLL 139
           CWVLWAHRMAFG RLF +VGKPG ALSE YLLSQS QGCFPMADYVFFQFAFAAITVVLL
Sbjct: 76  CWVLWAHRMAFGDRLFWLVGKPGAALSERYLLSQSPQGCFPMADYVFFQFAFAAITVVLL 135

Query: 140 AGSLLGRMNFGAWMLFVPMWLTLCYTVGAFCIWGDGFIQKYLIDYAGGYVIHLSSGVAGF 199
           AGSLLGRMNF AWMLFVPMWLTLCYTVGAFCIWG+GFIQKYLIDYAGGYVIHLSSGVAGF
Sbjct: 136 AGSLLGRMNFYAWMLFVPMWLTLCYTVGAFCIWGNGFIQKYLIDYAGGYVIHLSSGVAGF 195

Query: 200 TAAYWVGPRHSHDRQNFPPNNILHMVGGAGFLWLGWTGFNGGAPFAASRITSLAILNTHV 259
           TAAYWVGPRHS DRQNFPPNNILHMVGGAGFLWLGWTGFNGGAPFAASRITSLAILNTH+
Sbjct: 196 TAAYWVGPRHSQDRQNFPPNNILHMVGGAGFLWLGWTGFNGGAPFAASRITSLAILNTHI 255

Query: 260 CTATSLLIWVSMDIIVYKKSSVIGAVQGIITGLVCITPGAGLVDTIAAVVMGVLAGLVPW 319
           CTATSLLIWVSMDIIVYKKSSVIGAVQGIITGLVCITPGAGLVD +AAVVMGVLAGLVPW
Sbjct: 256 CTATSLLIWVSMDIIVYKKSSVIGAVQGIITGLVCITPGAGLVDPVAAVVMGVLAGLVPW 315

Query: 320 YTMMVLHRRLAFLQCVDDTLGVFHTHAVAGSMGGLLTGFFAKPSLLKMLYPDSLYGPGFL 379
           YTMMVLHRRLAFLQCVDDTLGVFHTHAVAG MGGLLTG FAKP+LL+M+Y D++YGPG L
Sbjct: 316 YTMMVLHRRLAFLQCVDDTLGVFHTHAVAGCMGGLLTGLFAKPNLLRMMYTDTIYGPGLL 375

Query: 380 YAISDKKLKDGLKQIGIQFAGAVFITLWNAIFTSIICYLISHIVNLRMKEEDLEIGDDAA 439
           YAISD+ LKDGLKQIGIQFAGAVFITLWNA+FTSIICYL+S IVNLRMKEEDLEIGDDAA
Sbjct: 376 YAISDRNLKDGLKQIGIQFAGAVFITLWNAVFTSIICYLVSLIVNLRMKEEDLEIGDDAA 435

Query: 440 HGEEPYALWGDGEKMPSSFRLPHTPRFPSLCRWD 474
           HGEEPYALWGDGEKMPSSFRLPHTPR PS CRWD
Sbjct: 436 HGEEPYALWGDGEKMPSSFRLPHTPRLPSFCRWD 469

BLAST of Cmc08g0217751 vs. ExPASy TrEMBL
Match: A0A6P6FTN3 (Ammonium transporter OS=Ziziphus jujuba OX=326968 GN=LOC107406851 PE=3 SV=1)

HSP 1 Score: 709.1 bits (1829), Expect = 1.2e-200
Identity = 336/460 (73.04%), Postives = 395/460 (85.87%), Query Frame = 0

Query: 12  LPPGFVDPEQTSPIWNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKKKWAVNSAFMAL 71
           LP G + P+  SP W +KADNAWQLTA TMVG+Q+VPGL+ILYGSMVKKKWAVNSAFMAL
Sbjct: 12  LPEGLL-PDPASPEWLSKADNAWQLTATTMVGVQSVPGLIILYGSMVKKKWAVNSAFMAL 71

Query: 72  YGFAAALVCWVLWAHRMAFGKRLFGIVGKPGLALSETYLLSQSTQGCFPMADYVFFQFAF 131
           Y FA  LVCWVLWAH M+FG +L  IVGKPGLA+++ +LL Q   G  PMAD+V+++F F
Sbjct: 72  YAFAVVLVCWVLWAHHMSFGDKLCSIVGKPGLAVTKKFLLRQFKYGYIPMADFVYYEFVF 131

Query: 132 AAITVVLLAGSLLGRMNFGAWMLFVPMWLTLCYTVGAFCIWGDGFIQKYLIDYAGGYVIH 191
           AAITV+LLAGSLLGRMNF AWM FVP+WLT CYTVGAF +WGDGF++KY+IDYAGGYVIH
Sbjct: 132 AAITVILLAGSLLGRMNFYAWMWFVPLWLTFCYTVGAFSVWGDGFLEKYIIDYAGGYVIH 191

Query: 192 LSSGVAGFTAAYWVGPRHSHDRQNFPPNNILHMVGGAGFLWLGWTGFNGGAPFAASRITS 251
           LSSGVAGF AAYWVGPR +HDRQ+FPPNNI+HM+ GAGFLWLGWTGFNGG+PFAA+ I S
Sbjct: 192 LSSGVAGFVAAYWVGPRQTHDRQHFPPNNIIHMLAGAGFLWLGWTGFNGGSPFAANEIAS 251

Query: 252 LAILNTHVCTATSLLIWVSMDIIVYKKSSVIGAVQGIITGLVCITPGAGLVDTIAAVVMG 311
           LAILNTH+CTATSLL+WVS+D++VY+KSSVIGAVQG+ITGLVCITPGAG+V+  AA++MG
Sbjct: 252 LAILNTHICTATSLLVWVSLDMVVYRKSSVIGAVQGMITGLVCITPGAGIVEPWAAIIMG 311

Query: 312 VLAGLVPWYTMMVLHRRLAFLQCVDDTLGVFHTHAVAGSMGGLLTGFFAKPSLLKMLY-P 371
           VL+G +PWYTMMVLHRR AF Q VDDTLGVFHTHAVAG +GG+L+G FAKP++L+M+Y  
Sbjct: 312 VLSGWIPWYTMMVLHRRSAFFQSVDDTLGVFHTHAVAGFLGGILSGIFAKPNVLEMMYGT 371

Query: 372 DSLYGPGFLYAISDKKLKDGLKQIGIQFAGAVFITLWNAIFTSIICYLISHIVNLRMKEE 431
            + +GPG  Y+I D KLKDGLKQ+G Q  GA FIT+WNA+ TS+IC+LIS IV+LRM EE
Sbjct: 372 ANPHGPGLFYSILDGKLKDGLKQMGYQLVGAAFITIWNAVVTSLICFLISFIVDLRMSEE 431

Query: 432 DLEIGDDAAHGEEPYALWGDGEKMPSSFRLPHTPRFPSLC 471
           DLEIGDDA HGEE YALWGDGE+MP S R     R PS+C
Sbjct: 432 DLEIGDDAVHGEEVYALWGDGERMPGSLRWNMPQRIPSIC 470

BLAST of Cmc08g0217751 vs. TAIR 10
Match: AT2G38290.1 (ammonium transporter 2 )

HSP 1 Score: 516.2 bits (1328), Expect = 2.9e-146
Identity = 262/457 (57.33%), Postives = 331/457 (72.43%), Query Frame = 0

Query: 15  GFVDPEQTS-PIWNNKADNAWQLTAATMVGLQTVPGLVILYGSMVKKKWAVNSAFMALYG 74
           G  DP     P W NK DNAWQLTAAT+VGLQ++PGLVILY S+VKKKWAVNSAFMALY 
Sbjct: 3   GAYDPSLPEVPEWLNKGDNAWQLTAATLVGLQSMPGLVILYASIVKKKWAVNSAFMALYA 62

Query: 75  FAAALVCWVLWAHRMAFGKRLFGIVGKPGLALSETYLLSQS-------TQGCFPMADYVF 134
           FAA L+CWVL  ++MAFG+ L    GK G A  + YL  Q+           FPMA  V+
Sbjct: 63  FAAVLLCWVLLCYKMAFGEELLPFWGKGGPAFDQGYLKGQAKIPNSNVAAPYFPMATLVY 122

Query: 135 FQFAFAAITVVLLAGSLLGRMNFGAWMLFVPMWLTLCYTVGAFCIWGDGFIQKY-LIDYA 194
           FQF FAAIT +L+AGS+LGRMN  AWM FVP+WL   YTVGA+ IWG GF+ ++ +IDY+
Sbjct: 123 FQFTFAAITTILVAGSVLGRMNIKAWMAFVPLWLIFSYTVGAYSIWGGGFLYQWGVIDYS 182

Query: 195 GGYVIHLSSGVAGFTAAYWVGPRHSHDRQNFPPNNILHMVGGAGFLWLGWTGFNGGAPFA 254
           GGYVIHLSSGVAGF AAYWVGPR   DR+ FPPNN+L M+ GAG LW+GW+GFNGGAP+A
Sbjct: 183 GGYVIHLSSGVAGFVAAYWVGPRPKADRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPYA 242

Query: 255 ASRITSLAILNTHVCTATSLLIWVSMDIIVYKKSSVIGAVQGIITGLVCITPGAGLVDTI 314
           A+  +S+A+LNT++  ATSLL+W ++D+I + K SVIGA+QG++TGL  +TPGAGL+ T 
Sbjct: 243 ANLTSSIAVLNTNLSAATSLLVWTTLDVIFFGKPSVIGAIQGMVTGLAGVTPGAGLIQTW 302

Query: 315 AAVVMGVLAGLVPWYTMMVLHRRLAFLQCVDDTLGVFHTHAVAGSMGGLLTGFFAKPSLL 374
           AA+++GV++G  PW +MM++H++ A LQ VDDTL VF+THAVAG +GG++TG FA P L 
Sbjct: 303 AAIIIGVVSGTAPWASMMIIHKKSALLQKVDDTLAVFYTHAVAGLLGGIMTGLFAHPDLC 362

Query: 375 KMLYP-----DSLYGPGFLYAISDKKLKDGLKQIGIQFAGAVFITLWNAIFTSIICYLIS 434
            ++ P      + YG             +G KQ+  Q AGA FI +WN + T+II   I 
Sbjct: 363 VLVLPLPATRGAFYG------------GNGGKQLLKQLAGAAFIAVWNVVSTTIILLAIR 422

Query: 435 HIVNLRMKEEDLEIGDDAAHGEEPYALWGDGEKMPSS 458
             + LRM EE+L IGDDAAHGEE YALWGDGEK  ++
Sbjct: 423 VFIPLRMAEEELGIGDDAAHGEEAYALWGDGEKFDAT 447

BLAST of Cmc08g0217751 vs. TAIR 10
Match: AT2G38290.2 (ammonium transporter 2 )

HSP 1 Score: 396.7 bits (1018), Expect = 2.5e-110
Identity = 196/343 (57.14%), Postives = 255/343 (74.34%), Query Frame = 0

Query: 121 MADYVFFQFAFAAITVVLLAGSLLGRMNFGAWMLFVPMWLTLCYTVGAFCIWGDGFIQKY 180
           MA  V+FQF FAAIT +L+AGS+LGRMN  AWM FVP+WL   YTVGA+ IWG GF+ ++
Sbjct: 1   MATLVYFQFTFAAITTILVAGSVLGRMNIKAWMAFVPLWLIFSYTVGAYSIWGGGFLYQW 60

Query: 181 -LIDYAGGYVIHLSSGVAGFTAAYWVGPRHSHDRQNFPPNNILHMVGGAGFLWLGWTGFN 240
            +IDY+GGYVIHLSSGVAGF AAYWVGPR   DR+ FPPNN+L M+ GAG LW+GW+GFN
Sbjct: 61  GVIDYSGGYVIHLSSGVAGFVAAYWVGPRPKADRERFPPNNVLLMLAGAGLLWMGWSGFN 120

Query: 241 GGAPFAASRITSLAILNTHVCTATSLLIWVSMDIIVYKKSSVIGAVQGIITGLVCITPGA 300
           GGAP+AA+  +S+A+LNT++  ATSLL+W ++D+I + K SVIGA+QG++TGL  +TPGA
Sbjct: 121 GGAPYAANLTSSIAVLNTNLSAATSLLVWTTLDVIFFGKPSVIGAIQGMVTGLAGVTPGA 180

Query: 301 GLVDTIAAVVMGVLAGLVPWYTMMVLHRRLAFLQCVDDTLGVFHTHAVAGSMGGLLTGFF 360
           GL+ T AA+++GV++G  PW +MM++H++ A LQ VDDTL VF+THAVAG +GG++TG F
Sbjct: 181 GLIQTWAAIIIGVVSGTAPWASMMIIHKKSALLQKVDDTLAVFYTHAVAGLLGGIMTGLF 240

Query: 361 AKPSLLKMLYP-----DSLYGPGFLYAISDKKLKDGLKQIGIQFAGAVFITLWNAIFTSI 420
           A P L  ++ P      + YG             +G KQ+  Q AGA FI +WN + T+I
Sbjct: 241 AHPDLCVLVLPLPATRGAFYG------------GNGGKQLLKQLAGAAFIAVWNVVSTTI 300

Query: 421 ICYLISHIVNLRMKEEDLEIGDDAAHGEEPYALWGDGEKMPSS 458
           I   I   + LRM EE+L IGDDAAHGEE YALWGDGEK  ++
Sbjct: 301 ILLAIRVFIPLRMAEEELGIGDDAAHGEEAYALWGDGEKFDAT 331

BLAST of Cmc08g0217751 vs. TAIR 10
Match: AT3G24300.1 (ammonium transporter 1;3 )

HSP 1 Score: 98.6 bits (244), Expect = 1.4e-20
Identity = 115/448 (25.67%), Postives = 188/448 (41.96%), Query Frame = 0

Query: 31  DNAWQLTAATMVGLQTVPGLVILYGSMVKKKWAVNSAFMALYGFAAALVCWVLWAHRMAF 90
           DN + L +A +V    + G  +L    V+ K  +N     +   AA  + + L+ +  AF
Sbjct: 46  DNTYLLFSAYLVFAMQL-GFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAF 105

Query: 91  GKRLFGIVGKPGLALSETYLLSQSTQGCFPMADYVFF--QFAFAAITVVLLAGSLLGRMN 150
           G    G +G+   AL +            P ADY FF  Q+AFA     + +GS+  R  
Sbjct: 106 GGSSEGFIGRHNFALRDFPT---------PTADYSFFLYQWAFAIAAAGITSGSIAERTQ 165

Query: 151 FGAWMLFVPMWLTLCYTVGAFCIWG-DGFIQKY-----------LIDYAGGYVIHLSSGV 210
           F A++++        Y V +   W  DG+   +            ID+AG  V+H+  G+
Sbjct: 166 FVAYLIYSSFLTGFVYPVVSHWFWSPDGWASPFRSADDRLFSTGAIDFAGSGVVHMVGGI 225

Query: 211 AGFTAAYWVGPRHSHDRQN-----FPPNNILHMVGGAGFLWLGWTGFNGGA------PFA 270
           AG   A   GPR     +         ++   +V G   LW GW GFN G+      P+ 
Sbjct: 226 AGLWGALIEGPRRGRFEKGGRAIALRGHSASLVVLGTFLLWFGWYGFNPGSFTKILVPYN 285

Query: 271 A-------SRITSLAILNTHVCTATSLLIWVSMDIIVYKKSSVIGAVQGIITGLVCITPG 330
           +       S I   A+ NT +   T+ L  +    ++    +V     G++ G   IT G
Sbjct: 286 SGSNYGQWSGIGRTAV-NTTLSGCTAALTTLFGKRLLSGHWNVTDVCNGLLGGFAAITAG 345

Query: 331 AGLVDTIAAVVMGVLAGLVPWYTMMVLHRRLAFLQCVDDTLGVFHTHAVAGSMGGLLTGF 390
             +V+  AA+V G +A +V     ++   +LA L   DD L     H   G+ G +  G 
Sbjct: 346 CSVVEPWAAIVCGFMASVV-----LIGCNKLAELVQYDDPLEAAQLHGGCGAWGLIFVGL 405

Query: 391 FAKPSLLKMLYPDSLYGPGFLYAISDKKLKDGLKQIGIQFAGAVFITLWNAIFTSIICYL 446
           FAK   L  +Y  +   PG  Y +    +  G K +G Q    + I  W +     + ++
Sbjct: 406 FAKEKYLNEVYGAT---PGRPYGLF---MGGGGKLLGAQLVQILVIVGWVSATMGTLFFI 465

BLAST of Cmc08g0217751 vs. TAIR 10
Match: AT3G24290.1 (ammonium transporter 1;5 )

HSP 1 Score: 96.7 bits (239), Expect = 5.4e-20
Identity = 118/451 (26.16%), Postives = 187/451 (41.46%), Query Frame = 0

Query: 31  DNAWQLTAATMVGLQTVPGLVILYGSMVKKKWAVNSAFMALYGFAAALVCWVLWAHRMAF 90
           DN + L +A +V    + G  +L    V+ K  +N     +   AA  + + L+ +  AF
Sbjct: 46  DNTYLLFSAYLVFAMQL-GFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAF 105

Query: 91  GKRLFGIVGKPGLALSETYLLSQSTQGCFPMADYVFF--QFAFAAITVVLLAGSLLGRMN 150
           G+   G +G+    L     L+         +DY FF  Q+AFA     + +GS+  R  
Sbjct: 106 GESSDGFIGRHNFGLQNFPTLT---------SDYSFFLYQWAFAIAAAGITSGSIAERTK 165

Query: 151 FGAWMLFVPMWLTLCYTVGAFCIWG-DGFIQKY----------LIDYAGGYVIHLSSGVA 210
           F A++++        Y V +   W  DG+   +           ID+AG  V+H+  G+A
Sbjct: 166 FVAYLIYSSFLTGFVYPVVSHWFWSPDGWASPFRSEDRLFGTGAIDFAGSGVVHMVGGIA 225

Query: 211 GFTAAYWVGPRHSHDRQNFPP---------NNILHMVGGAGFLWLGWTGFNGGA------ 270
           G   A   GPR       FP          ++   +V G   LW GW GFN G+      
Sbjct: 226 GLWGALIEGPRIG----RFPDGGHAIALRGHSASLVVLGTFLLWFGWYGFNPGSFTKILI 285

Query: 271 PFAA-------SRITSLAILNTHVCTATSLLIWVSMDIIVYKKSSVIGAVQGIITGLVCI 330
           P+ +       S I   A+  T +   T+ L  +    ++    +V     G++ G   I
Sbjct: 286 PYNSGSNYGQWSGIGRTAVTTT-LSGCTAALTTLFGKRLLSGHWNVTDVCNGLLGGFAAI 345

Query: 331 TPGAGLVDTIAAVVMGVLAGLVPWYTMMVLHRRLAFLQCVDDTLGVFHTHAVAGSMGGLL 390
           T G  +VD  AA+V G +A LV     ++   +LA L   DD L     H   G+ G + 
Sbjct: 346 TAGCSVVDPWAAIVCGFVASLV-----LIGCNKLAELLKYDDPLEAAQLHGGCGAWGLIF 405

Query: 391 TGFFAKPSLLKMLYPDSLYGPGFLYAISDKKLKDGLKQIGIQFAGAVFITLWNAIFTSII 446
            G FAK   +  +Y  S   PG  Y +    +  G K +G Q    + I  W +     +
Sbjct: 406 VGLFAKEKYINEVYGAS---PGRHYGLF---MGGGGKLLGAQLVQIIVIVGWVSATMGTL 465

BLAST of Cmc08g0217751 vs. TAIR 10
Match: AT4G13510.1 (ammonium transporter 1;1 )

HSP 1 Score: 94.0 bits (232), Expect = 3.5e-19
Identity = 116/471 (24.63%), Postives = 184/471 (39.07%), Query Frame = 0

Query: 31  DNAWQLTAATMVGLQTVPGLVILYGSMVKKKWAVNSAFMALYGFAAALVCWVLWAHRMAF 90
           DN + L +A +V    + G  +L    V+ K  +N     +   AA  + + L+ +  AF
Sbjct: 42  DNTYLLFSAYLVFSMQL-GFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAF 101

Query: 91  GKRLFGIVGKPGLALSETYLLSQSTQGCFPMADY--VFFQFAFAAITVVLLAGSLLGRMN 150
           G    G +GK    L +    S         ADY    +Q+AFA     + +GS+  R  
Sbjct: 102 GSPSNGFIGKHYFGLKDIPTAS---------ADYSNFLYQWAFAIAAAGITSGSIAERTQ 161

Query: 151 FGAWMLFVPMWLTLCYTVGAFCIW------------GDGFIQKYLIDYAGGYVIHLSSGV 210
           F A++++        Y V +   W            GD       ID+AG  V+H+  G+
Sbjct: 162 FVAYLIYSSFLTGFVYPVVSHWFWSVDGWASPFRTDGDLLFSTGAIDFAGSGVVHMVGGI 221

Query: 211 AGFTAAYWVGPRHSHDRQN-----FPPNNILHMVGGAGFLWLGWTGFNGGAPFAASRIT- 270
           AG   A   GPR               ++   +V G   LW GW GFN G+ F    +T 
Sbjct: 222 AGLWGALIEGPRLGRFDNGGRAIALRGHSASLVVLGTFLLWFGWYGFNPGS-FNKILVTY 281

Query: 271 ------------SLAILNTHVCTATSLLIWVSMDIIVYKKSSVIGAVQGIITGLVCITPG 330
                           + T +   T+ L  +    ++    +V     G++ G   IT G
Sbjct: 282 ETGTYNGQWSAVGRTAVTTTLAGCTAALTTLFGKRLLSGHWNVTDVCNGLLGGFAAITGG 341

Query: 331 AGLVDTIAAVVMGVLAGLVPWYTMMVLHRRLAFLQCVDDTLGVFHTHAVAGSMGGLLTGF 390
             +V+  AA++ G +A LV     ++   +LA     DD L     H   G+ G + T  
Sbjct: 342 CSVVEPWAAIICGFVAALV-----LLGCNKLAEKLKYDDPLEAAQLHGGCGAWGLIFTAL 401

Query: 391 FAKPSLLKMLYPDSLYGPGFLYAISDKKLKDGLKQIGIQFAGAVFITLWNAIFTSIICYL 450
           FA+   L  +Y +    P  L+      +  G K +G Q    + IT W +     + ++
Sbjct: 402 FAQEKYLNQIYGNKPGRPHGLF------MGGGGKLLGAQLIQIIVITGWVSATMGTLFFI 461

Query: 451 ISHIVNLRMKEEDLEIG-DDAAHGEEPYALWGDGEKMPSSFRLPHTPRFPS 469
           +  +  LR+  ED   G D   HG   Y  + D E   +       PR PS
Sbjct: 462 LKKMKLLRISSEDEMAGMDMTRHGGFAYMYFDDDESHKAIQLRRVEPRSPS 490

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008440880.11.3e-27394.41PREDICTED: LOW QUALITY PROTEIN: ammonium transporter 3 member 2-like [Cucumis me... [more]
XP_004135012.14.9e-27397.89ammonium transporter 3 member 3 [Cucumis sativus] >KAE8647622.1 hypothetical pro... [more]
KAA0025632.12.7e-271100.00ammonium transporter 3 member 3-like [Cucumis melo var. makuwa] >TYK12505.1 ammo... [more]
XP_038881912.11.3e-26294.50ammonium transporter 2 member 3-like [Benincasa hispida][more]
KAG6603880.15.4e-25692.18Ammonium transporter 3 member 1, partial [Cucurbita argyrosperma subsp. sororia]... [more]
Match NameE-valueIdentityDescription
G7LAA81.4e-19071.56Ammonium transporter 2 member 3 OS=Medicago truncatula OX=3880 GN=AMT2-3 PE=2 SV... [more]
A0A072VHJ19.6e-15560.09Ammonium transporter 2 member 5 OS=Medicago truncatula OX=3880 GN=AMT2-5 PE=2 SV... [more]
Q84KJ63.5e-14957.57Ammonium transporter 3 member 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT3... [more]
Q69T291.8e-14858.19Ammonium transporter 3 member 3 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT3... [more]
Q851M98.7e-14858.52Ammonium transporter 3 member 2 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT3... [more]
Match NameE-valueIdentityDescription
A0A1S3B2666.2e-27494.41LOW QUALITY PROTEIN: ammonium transporter 3 member 2-like OS=Cucumis melo OX=365... [more]
A0A5D3CLR11.3e-271100.00Ammonium transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255... [more]
A0A0A0KHE11.6e-25897.79Ammonium transporter OS=Cucumis sativus OX=3659 GN=Csa_6G507360 PE=3 SV=1[more]
A0A6J1BTX02.0e-24892.73Ammonium transporter OS=Momordica charantia OX=3673 GN=LOC111005728 PE=3 SV=1[more]
A0A6P6FTN31.2e-20073.04Ammonium transporter OS=Ziziphus jujuba OX=326968 GN=LOC107406851 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G38290.12.9e-14657.33ammonium transporter 2 [more]
AT2G38290.22.5e-11057.14ammonium transporter 2 [more]
AT3G24300.11.4e-2025.67ammonium transporter 1;3 [more]
AT3G24290.15.4e-2026.16ammonium transporter 1;5 [more]
AT4G13510.13.5e-1924.63ammonium transporter 1;1 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002229Blood group Rhesus C/E/D polypeptidePRINTSPR00342RHESUSRHDcoord: 308..326
score: 27.78
coord: 253..275
score: 26.09
coord: 222..240
score: 42.69
coord: 128..144
score: 24.18
IPR029020Ammonium/urea transporterGENE3D1.10.3430.10Ammonium transporter AmtB like domainscoord: 17..448
e-value: 1.7E-111
score: 375.0
IPR001905Ammonium transporterTIGRFAMTIGR00836TIGR00836coord: 31..445
e-value: 1.6E-107
score: 357.9
IPR024041Ammonium transporter AmtB-like domainPFAMPF00909Ammonium_transpcoord: 33..445
e-value: 3.8E-77
score: 259.6
NoneNo IPR availablePANTHERPTHR43029:SF24AMMONIUM TRANSPORTERcoord: 11..471
NoneNo IPR availablePANTHERPTHR43029AMMONIUM TRANSPORTER MEP2coord: 11..471
NoneNo IPR availableSUPERFAMILY111352Ammonium transportercoord: 26..427
IPR018047Ammonium transporter, conserved sitePROSITEPS01219AMMONIUM_TRANSPcoord: 183..208

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc08g0217751.1Cmc08g0217751.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0072488 ammonium transmembrane transport
biological_process GO:0015696 ammonium transport
cellular_component GO:0005886 plasma membrane
cellular_component GO:0005887 integral component of plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0008519 ammonium transmembrane transporter activity