Cmc08g0211861 (gene) Melon (Charmono) v1.1

Overview
NameCmc08g0211861
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Description(1->3)-beta-glucan endohydrolase
LocationCMiso1.1chr08: 2981690 .. 2984087 (+)
RNA-Seq ExpressionCmc08g0211861
SyntenyCmc08g0211861
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTAAAAATAAAATCAAATCCCTTTTCTGTCCTGAAGAATCGGCGGCCTGAAGAAGACCGAATGACCGGGGTGTCGATCGTCCCCGCCGTGTGGGCGGCCGTAATGACAGTGGCAGCGGTGTTTTCGGTGGTTGAAGGGGCCGGAATCGGGGCGAATTGGGGAATAATGTCATCGCACCCACTGCGTCCGAACATTGTGGTGAAATTGCTGAAAGATAATGGGATTAAAAAAGTGAAGCTGTTCGATTCGGATTCATGGACTGTGAGTGCGTTGAGTGGTTCAAAAATTGAAACGGTCATTGGAATCCCTAACGATCAGCTTAAGAAATTTGCGGAAAGTTACGATGACGCTAAAGATTGGGTTAAAGAAAACGTTACCACTCACATGTTTGAAGGCGGTGTTGATCTCAGGTTTAACTTCCATCTCTTTTTAGTTAAATATTTAACACAAAACAATGTTTTCTAATATTCTAATTTTTTAAATTTTATTATCTAAATAGTTAGAACGAAGAATACAAAATTAAAATTTATGAGATTTTAAAAAGAAAAGGGACGTCATTATTAGTTCAATTGTATATCAACCATATTAAACTTTTTTTTTTATTTTATATACTAACGACATTTTGTACTCTTTTATAGGAGTGTCTCATGAAAAATAATAAAATTTTTTATAATAATAAAATTTCTATCACTATATTAAAATAACAAACTTAAAAGCAGATAATTCTTTGATAAAAATATATTGATTCATAGTACTATGTAAATGTGAAATATCAACGTATATTAAAATTAATACTATAAATAAAAAAGGGACAAAATGTTACCTTAAACCAATTTTTAGGATTGAATGTGATGTTTGGTATTATGATCTAAATCACATTCATACATGAAATTATGTTTATATATAATTTACACTTCCTTAACTTTGGACAAAAGAAAGTTCACATTAAATATTAATCCAAATGATATGATATATAAAAAAGGCATTTCGAATGATATGTTATTTAATGAAAAAAATCTAAGAATTTGTCGTACAATAACAATTTTTCAGGTACGTGTCTGTGGGAAACGAAGCTTTCTTGTCGGCTTACAATGGAACCTACGTGAAAGTCACTTATCCGGCAATGCAAAACATACAGAAAGCTCTCGACGCCGCCGGCCATGGCAAAAAAGTCAAGGTCACGACTGCTCTCAACGCCGACGTCTATGAATCCAGCACCAACCTACCTTCCGACGGCCAATTCCGACCAGACATTTACAAGACAATGAAGGATATAGTCCACTTCCTCGACCGTAACAAAGCCCCTTTCATGGTCAACATTTACCCTTTCCTCAGTCTTTACCAGAACCCTAATTTCCCCCTCGATTACGCCTTCTTCGGCGGCGGTGCTAAGGCCACCAACGACAAGGGCAAATCCTACACCAACGTCTTCGACGCCAATTACGACACACTCATTTGGTCCTTGAAGAAAATCGGCGTCACCGACATGAAAATTATCGTCGGAGAAGTCGGCTGGCCGACGGACGGCAACAAATTCGCGAATGTAGAACTTGCGAAACGATTCTACGACGGACTGTTCAAGAAATTGGCCTCCGGCAATGGAACTCCGATGAGGCCGAAGGAGAAATTCGAGGTTTATTTGTTCGGACTTTTAGATGAGGACATGAAAAGCGTTCTGCCAGGGTTTTTCGAGCGACATTGGGGGATTTTCCAATTCGACGGAAAACCTAAGTTTCCGATGGATATGTCGGGGAAAGGGAACGACAAAATGCTGGTGGCGGCGAAAGGAGTGCAGTATTTGGAGAAAAAATGGTGCGTTTTGAAGGAGAATGTAAAAAATTTCGATAAAATTTCGAAGGAGGTGGATTACGCTTGTGGTCTTTCGGATTGTACGAGTTTGGGATATGGATCGTCGTGCAACAATTTGGATAAGCGAGGGAATATTTCTTATGCGTTCAATATGTATTACCAGATGCAGGATCAGAGTGTGGAGGCCTGCGATTTCAGCGGATCCGCCCATATTGTCAAGAAAAATGCTTCGGTTGGAAGCTGCTTGTTTCCGATCCAGATTGTGAGCACCGGAGAGAGGTTGAAGGCGGCGGCGGCGGCGGCCGTGGTTGGGCTTGTGTTGGGCTTGTTTGCTTTAGGTCTTTGAAGGAGGAACTTTTTTCAGTTTCTTTTCGAGGGATTTCTTTAGGGTAATTTTGAGGGATTTCTTTTCATTTGTTATTTATTTATGGAATTGTATTTTGTAGGTTTGAAGGATAACAAATAACACTTGTTGTCCCAAAAATGAATACTTATACCACTTTTGAGAAAGGAAAAAACTAATATTTGTTTTTTAAATTTAGCTTTAATTTATATTAAAACATTAATTAAACTCACACAACAATAATG

mRNA sequence

TTTAAAAATAAAATCAAATCCCTTTTCTGTCCTGAAGAATCGGCGGCCTGAAGAAGACCGAATGACCGGGGTGTCGATCGTCCCCGCCGTGTGGGCGGCCGTAATGACAGTGGCAGCGGTGTTTTCGGTGGTTGAAGGGGCCGGAATCGGGGCGAATTGGGGAATAATGTCATCGCACCCACTGCGTCCGAACATTGTGGTGAAATTGCTGAAAGATAATGGGATTAAAAAAGTGAAGCTGTTCGATTCGGATTCATGGACTGTGAGTGCGTTGAGTGGTTCAAAAATTGAAACGGTCATTGGAATCCCTAACGATCAGCTTAAGAAATTTGCGGAAAGTTACGATGACGCTAAAGATTGGGTTAAAGAAAACGTTACCACTCACATGTTTGAAGGCGGTGTTGATCTCAGGTACGTGTCTGTGGGAAACGAAGCTTTCTTGTCGGCTTACAATGGAACCTACGTGAAAGTCACTTATCCGGCAATGCAAAACATACAGAAAGCTCTCGACGCCGCCGGCCATGGCAAAAAAGTCAAGGTCACGACTGCTCTCAACGCCGACGTCTATGAATCCAGCACCAACCTACCTTCCGACGGCCAATTCCGACCAGACATTTACAAGACAATGAAGGATATAGTCCACTTCCTCGACCGTAACAAAGCCCCTTTCATGGTCAACATTTACCCTTTCCTCAGTCTTTACCAGAACCCTAATTTCCCCCTCGATTACGCCTTCTTCGGCGGCGGTGCTAAGGCCACCAACGACAAGGGCAAATCCTACACCAACGTCTTCGACGCCAATTACGACACACTCATTTGGTCCTTGAAGAAAATCGGCGTCACCGACATGAAAATTATCGTCGGAGAAGTCGGCTGGCCGACGGACGGCAACAAATTCGCGAATGTAGAACTTGCGAAACGATTCTACGACGGACTGTTCAAGAAATTGGCCTCCGGCAATGGAACTCCGATGAGGCCGAAGGAGAAATTCGAGGTTTATTTGTTCGGACTTTTAGATGAGGACATGAAAAGCGTTCTGCCAGGGTTTTTCGAGCGACATTGGGGGATTTTCCAATTCGACGGAAAACCTAAGTTTCCGATGGATATGTCGGGGAAAGGGAACGACAAAATGCTGGTGGCGGCGAAAGGAGTGCAGTATTTGGAGAAAAAATGGTGCGTTTTGAAGGAGAATGTAAAAAATTTCGATAAAATTTCGAAGGAGGTGGATTACGCTTGTGGTCTTTCGGATTGTACGAGTTTGGGATATGGATCGTCGTGCAACAATTTGGATAAGCGAGGGAATATTTCTTATGCGTTCAATATGTATTACCAGATGCAGGATCAGAGTGTGGAGGCCTGCGATTTCAGCGGATCCGCCCATATTGTCAAGAAAAATGCTTCGGTTGGAAGCTGCTTGTTTCCGATCCAGATTGTGAGCACCGGAGAGAGGTTGAAGGCGGCGGCGGCGGCGGCCGTGGTTGGGCTTGTGTTGGGCTTGTTTGCTTTAGGTCTTTGAAGGAGGAACTTTTTTCAGTTTCTTTTCGAGGGATTTCTTTAGGGTAATTTTGAGGGATTTCTTTTCATTTGTTATTTATTTATGGAATTGTATTTTGTAGGTTTGAAGGATAACAAATAACACTTGTTGTCCCAAAAATGAATACTTATACCACTTTTGAGAAAGGAAAAAACTAATATTTGTTTTTTAAATTTAGCTTTAATTTATATTAAAACATTAATTAAACTCACACAACAATAATG

Coding sequence (CDS)

ATGACCGGGGTGTCGATCGTCCCCGCCGTGTGGGCGGCCGTAATGACAGTGGCAGCGGTGTTTTCGGTGGTTGAAGGGGCCGGAATCGGGGCGAATTGGGGAATAATGTCATCGCACCCACTGCGTCCGAACATTGTGGTGAAATTGCTGAAAGATAATGGGATTAAAAAAGTGAAGCTGTTCGATTCGGATTCATGGACTGTGAGTGCGTTGAGTGGTTCAAAAATTGAAACGGTCATTGGAATCCCTAACGATCAGCTTAAGAAATTTGCGGAAAGTTACGATGACGCTAAAGATTGGGTTAAAGAAAACGTTACCACTCACATGTTTGAAGGCGGTGTTGATCTCAGGTACGTGTCTGTGGGAAACGAAGCTTTCTTGTCGGCTTACAATGGAACCTACGTGAAAGTCACTTATCCGGCAATGCAAAACATACAGAAAGCTCTCGACGCCGCCGGCCATGGCAAAAAAGTCAAGGTCACGACTGCTCTCAACGCCGACGTCTATGAATCCAGCACCAACCTACCTTCCGACGGCCAATTCCGACCAGACATTTACAAGACAATGAAGGATATAGTCCACTTCCTCGACCGTAACAAAGCCCCTTTCATGGTCAACATTTACCCTTTCCTCAGTCTTTACCAGAACCCTAATTTCCCCCTCGATTACGCCTTCTTCGGCGGCGGTGCTAAGGCCACCAACGACAAGGGCAAATCCTACACCAACGTCTTCGACGCCAATTACGACACACTCATTTGGTCCTTGAAGAAAATCGGCGTCACCGACATGAAAATTATCGTCGGAGAAGTCGGCTGGCCGACGGACGGCAACAAATTCGCGAATGTAGAACTTGCGAAACGATTCTACGACGGACTGTTCAAGAAATTGGCCTCCGGCAATGGAACTCCGATGAGGCCGAAGGAGAAATTCGAGGTTTATTTGTTCGGACTTTTAGATGAGGACATGAAAAGCGTTCTGCCAGGGTTTTTCGAGCGACATTGGGGGATTTTCCAATTCGACGGAAAACCTAAGTTTCCGATGGATATGTCGGGGAAAGGGAACGACAAAATGCTGGTGGCGGCGAAAGGAGTGCAGTATTTGGAGAAAAAATGGTGCGTTTTGAAGGAGAATGTAAAAAATTTCGATAAAATTTCGAAGGAGGTGGATTACGCTTGTGGTCTTTCGGATTGTACGAGTTTGGGATATGGATCGTCGTGCAACAATTTGGATAAGCGAGGGAATATTTCTTATGCGTTCAATATGTATTACCAGATGCAGGATCAGAGTGTGGAGGCCTGCGATTTCAGCGGATCCGCCCATATTGTCAAGAAAAATGCTTCGGTTGGAAGCTGCTTGTTTCCGATCCAGATTGTGAGCACCGGAGAGAGGTTGAAGGCGGCGGCGGCGGCGGCCGTGGTTGGGCTTGTGTTGGGCTTGTTTGCTTTAGGTCTTTGA

Protein sequence

MTGVSIVPAVWAAVMTVAAVFSVVEGAGIGANWGIMSSHPLRPNIVVKLLKDNGIKKVKLFDSDSWTVSALSGSKIETVIGIPNDQLKKFAESYDDAKDWVKENVTTHMFEGGVDLRYVSVGNEAFLSAYNGTYVKVTYPAMQNIQKALDAAGHGKKVKVTTALNADVYESSTNLPSDGQFRPDIYKTMKDIVHFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFFGGGAKATNDKGKSYTNVFDANYDTLIWSLKKIGVTDMKIIVGEVGWPTDGNKFANVELAKRFYDGLFKKLASGNGTPMRPKEKFEVYLFGLLDEDMKSVLPGFFERHWGIFQFDGKPKFPMDMSGKGNDKMLVAAKGVQYLEKKWCVLKENVKNFDKISKEVDYACGLSDCTSLGYGSSCNNLDKRGNISYAFNMYYQMQDQSVEACDFSGSAHIVKKNASVGSCLFPIQIVSTGERLKAAAAAAVVGLVLGLFALGL
Homology
BLAST of Cmc08g0211861 vs. NCBI nr
Match: XP_008440124.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 8 [Cucumis melo])

HSP 1 Score: 979.9 bits (2532), Expect = 7.7e-282
Identity = 484/484 (100.00%), Postives = 484/484 (100.00%), Query Frame = 0

Query: 1   MTGVSIVPAVWAAVMTVAAVFSVVEGAGIGANWGIMSSHPLRPNIVVKLLKDNGIKKVKL 60
           MTGVSIVPAVWAAVMTVAAVFSVVEGAGIGANWGIMSSHPLRPNIVVKLLKDNGIKKVKL
Sbjct: 1   MTGVSIVPAVWAAVMTVAAVFSVVEGAGIGANWGIMSSHPLRPNIVVKLLKDNGIKKVKL 60

Query: 61  FDSDSWTVSALSGSKIETVIGIPNDQLKKFAESYDDAKDWVKENVTTHMFEGGVDLRYVS 120
           FDSDSWTVSALSGSKIETVIGIPNDQLKKFAESYDDAKDWVKENVTTHMFEGGVDLRYVS
Sbjct: 61  FDSDSWTVSALSGSKIETVIGIPNDQLKKFAESYDDAKDWVKENVTTHMFEGGVDLRYVS 120

Query: 121 VGNEAFLSAYNGTYVKVTYPAMQNIQKALDAAGHGKKVKVTTALNADVYESSTNLPSDGQ 180
           VGNEAFLSAYNGTYVKVTYPAMQNIQKALDAAGHGKKVKVTTALNADVYESSTNLPSDGQ
Sbjct: 121 VGNEAFLSAYNGTYVKVTYPAMQNIQKALDAAGHGKKVKVTTALNADVYESSTNLPSDGQ 180

Query: 181 FRPDIYKTMKDIVHFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFFGGGAKATNDKGKSY 240
           FRPDIYKTMKDIVHFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFFGGGAKATNDKGKSY
Sbjct: 181 FRPDIYKTMKDIVHFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFFGGGAKATNDKGKSY 240

Query: 241 TNVFDANYDTLIWSLKKIGVTDMKIIVGEVGWPTDGNKFANVELAKRFYDGLFKKLASGN 300
           TNVFDANYDTLIWSLKKIGVTDMKIIVGEVGWPTDGNKFANVELAKRFYDGLFKKLASGN
Sbjct: 241 TNVFDANYDTLIWSLKKIGVTDMKIIVGEVGWPTDGNKFANVELAKRFYDGLFKKLASGN 300

Query: 301 GTPMRPKEKFEVYLFGLLDEDMKSVLPGFFERHWGIFQFDGKPKFPMDMSGKGNDKMLVA 360
           GTPMRPKEKFEVYLFGLLDEDMKSVLPGFFERHWGIFQFDGKPKFPMDMSGKGNDKMLVA
Sbjct: 301 GTPMRPKEKFEVYLFGLLDEDMKSVLPGFFERHWGIFQFDGKPKFPMDMSGKGNDKMLVA 360

Query: 361 AKGVQYLEKKWCVLKENVKNFDKISKEVDYACGLSDCTSLGYGSSCNNLDKRGNISYAFN 420
           AKGVQYLEKKWCVLKENVKNFDKISKEVDYACGLSDCTSLGYGSSCNNLDKRGNISYAFN
Sbjct: 361 AKGVQYLEKKWCVLKENVKNFDKISKEVDYACGLSDCTSLGYGSSCNNLDKRGNISYAFN 420

Query: 421 MYYQMQDQSVEACDFSGSAHIVKKNASVGSCLFPIQIVSTGERLKAAAAAAVVGLVLGLF 480
           MYYQMQDQSVEACDFSGSAHIVKKNASVGSCLFPIQIVSTGERLKAAAAAAVVGLVLGLF
Sbjct: 421 MYYQMQDQSVEACDFSGSAHIVKKNASVGSCLFPIQIVSTGERLKAAAAAAVVGLVLGLF 480

Query: 481 ALGL 485
           ALGL
Sbjct: 481 ALGL 484

BLAST of Cmc08g0211861 vs. NCBI nr
Match: TYK12975.1 (tRNA(His) guanylyltransferase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 979.9 bits (2532), Expect = 7.7e-282
Identity = 484/484 (100.00%), Postives = 484/484 (100.00%), Query Frame = 0

Query: 1   MTGVSIVPAVWAAVMTVAAVFSVVEGAGIGANWGIMSSHPLRPNIVVKLLKDNGIKKVKL 60
           MTGVSIVPAVWAAVMTVAAVFSVVEGAGIGANWGIMSSHPLRPNIVVKLLKDNGIKKVKL
Sbjct: 1   MTGVSIVPAVWAAVMTVAAVFSVVEGAGIGANWGIMSSHPLRPNIVVKLLKDNGIKKVKL 60

Query: 61  FDSDSWTVSALSGSKIETVIGIPNDQLKKFAESYDDAKDWVKENVTTHMFEGGVDLRYVS 120
           FDSDSWTVSALSGSKIETVIGIPNDQLKKFAESYDDAKDWVKENVTTHMFEGGVDLRYVS
Sbjct: 61  FDSDSWTVSALSGSKIETVIGIPNDQLKKFAESYDDAKDWVKENVTTHMFEGGVDLRYVS 120

Query: 121 VGNEAFLSAYNGTYVKVTYPAMQNIQKALDAAGHGKKVKVTTALNADVYESSTNLPSDGQ 180
           VGNEAFLSAYNGTYVKVTYPAMQNIQKALDAAGHGKKVKVTTALNADVYESSTNLPSDGQ
Sbjct: 121 VGNEAFLSAYNGTYVKVTYPAMQNIQKALDAAGHGKKVKVTTALNADVYESSTNLPSDGQ 180

Query: 181 FRPDIYKTMKDIVHFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFFGGGAKATNDKGKSY 240
           FRPDIYKTMKDIVHFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFFGGGAKATNDKGKSY
Sbjct: 181 FRPDIYKTMKDIVHFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFFGGGAKATNDKGKSY 240

Query: 241 TNVFDANYDTLIWSLKKIGVTDMKIIVGEVGWPTDGNKFANVELAKRFYDGLFKKLASGN 300
           TNVFDANYDTLIWSLKKIGVTDMKIIVGEVGWPTDGNKFANVELAKRFYDGLFKKLASGN
Sbjct: 241 TNVFDANYDTLIWSLKKIGVTDMKIIVGEVGWPTDGNKFANVELAKRFYDGLFKKLASGN 300

Query: 301 GTPMRPKEKFEVYLFGLLDEDMKSVLPGFFERHWGIFQFDGKPKFPMDMSGKGNDKMLVA 360
           GTPMRPKEKFEVYLFGLLDEDMKSVLPGFFERHWGIFQFDGKPKFPMDMSGKGNDKMLVA
Sbjct: 301 GTPMRPKEKFEVYLFGLLDEDMKSVLPGFFERHWGIFQFDGKPKFPMDMSGKGNDKMLVA 360

Query: 361 AKGVQYLEKKWCVLKENVKNFDKISKEVDYACGLSDCTSLGYGSSCNNLDKRGNISYAFN 420
           AKGVQYLEKKWCVLKENVKNFDKISKEVDYACGLSDCTSLGYGSSCNNLDKRGNISYAFN
Sbjct: 361 AKGVQYLEKKWCVLKENVKNFDKISKEVDYACGLSDCTSLGYGSSCNNLDKRGNISYAFN 420

Query: 421 MYYQMQDQSVEACDFSGSAHIVKKNASVGSCLFPIQIVSTGERLKAAAAAAVVGLVLGLF 480
           MYYQMQDQSVEACDFSGSAHIVKKNASVGSCLFPIQIVSTGERLKAAAAAAVVGLVLGLF
Sbjct: 421 MYYQMQDQSVEACDFSGSAHIVKKNASVGSCLFPIQIVSTGERLKAAAAAAVVGLVLGLF 480

Query: 481 ALGL 485
           ALGL
Sbjct: 481 ALGL 484

BLAST of Cmc08g0211861 vs. NCBI nr
Match: XP_004142041.1 (glucan endo-1,3-beta-glucosidase 8 [Cucumis sativus] >KGN48417.1 hypothetical protein Csa_004042 [Cucumis sativus])

HSP 1 Score: 902.5 bits (2331), Expect = 1.6e-258
Identity = 441/484 (91.12%), Postives = 464/484 (95.87%), Query Frame = 0

Query: 1   MTGVSIVPAVWAAVMTVAAVFSVVEGAGIGANWGIMSSHPLRPNIVVKLLKDNGIKKVKL 60
           M GVS+VPAVWAAVMT+ AVFSVVEGAGIG NWGI+SSHPLRPNIVVKLLKDNGIKKVKL
Sbjct: 1   MAGVSMVPAVWAAVMTLGAVFSVVEGAGIGVNWGILSSHPLRPNIVVKLLKDNGIKKVKL 60

Query: 61  FDSDSWTVSALSGSKIETVIGIPNDQLKKFAESYDDAKDWVKENVTTHMFEGGVDLRYVS 120
           FDSD+WTVS+LSGSKIET+IGIPNDQLKKFA+SYDDAKDWVKENVT+HMFEGGVDLRYVS
Sbjct: 61  FDSDAWTVSSLSGSKIETIIGIPNDQLKKFAKSYDDAKDWVKENVTSHMFEGGVDLRYVS 120

Query: 121 VGNEAFLSAYNGTYVKVTYPAMQNIQKALDAAGHGKKVKVTTALNADVYESSTNLPSDGQ 180
           VGNEAFL+AYNG++VKVT+PAMQNIQKA+DAAGHGKK+KVTTALNADVYESSTNLPSDG+
Sbjct: 121 VGNEAFLTAYNGSFVKVTFPAMQNIQKAIDAAGHGKKIKVTTALNADVYESSTNLPSDGE 180

Query: 181 FRPDIYKTMKDIVHFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFFGGGAKATNDKGKSY 240
           FRPDIY TMKDIVHFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFF GG KATNDK KSY
Sbjct: 181 FRPDIYSTMKDIVHFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFFDGGGKATNDKDKSY 240

Query: 241 TNVFDANYDTLIWSLKKIGVTDMKIIVGEVGWPTDGNKFANVELAKRFYDGLFKKLASGN 300
           TNVFDANYDTLIWSLKK+GV+DMKIIVGEVGWPTDGNKFANV LAKRFYDGLFKKLASG+
Sbjct: 241 TNVFDANYDTLIWSLKKLGVSDMKIIVGEVGWPTDGNKFANVNLAKRFYDGLFKKLASGD 300

Query: 301 GTPMRPKEKFEVYLFGLLDEDMKSVLPGFFERHWGIFQFDGKPKFPMDMSGKGNDKMLVA 360
           GTPMRPKEKFEVYLFGLLDEDMKSVLPGFFERHWGIFQFDGKPKFPMD SGKGNDKMLVA
Sbjct: 301 GTPMRPKEKFEVYLFGLLDEDMKSVLPGFFERHWGIFQFDGKPKFPMDSSGKGNDKMLVA 360

Query: 361 AKGVQYLEKKWCVLKENVKNFDKISKEVDYACGLSDCTSLGYGSSCNNLDKRGNISYAFN 420
           AKGVQYLEKKWCVLK+NVK FD IS +VDYAC LSDCTSLGYGSSCNNLD+RGNISYAFN
Sbjct: 361 AKGVQYLEKKWCVLKKNVKVFDNISPQVDYACSLSDCTSLGYGSSCNNLDRRGNISYAFN 420

Query: 421 MYYQMQDQSVEACDFSGSAHIVKKNASVGSCLFPIQIVSTGERLKAAAAAAVVGLVLGLF 480
           MYYQMQDQSVEAC F+GSA IVK NASVGSCLFPIQIVS GERLK AAAAA+VGLVL LF
Sbjct: 421 MYYQMQDQSVEACVFNGSAEIVKNNASVGSCLFPIQIVSAGERLK-AAAAAMVGLVLSLF 480

Query: 481 ALGL 485
           AL L
Sbjct: 481 ALRL 483

BLAST of Cmc08g0211861 vs. NCBI nr
Match: XP_038883272.1 (glucan endo-1,3-beta-glucosidase 8 [Benincasa hispida])

HSP 1 Score: 828.9 bits (2140), Expect = 2.2e-236
Identity = 404/484 (83.47%), Postives = 438/484 (90.50%), Query Frame = 0

Query: 1   MTGVSIVPAVWAAVMTVAAVFSVVEGAGIGANWGIMSSHPLRPNIVVKLLKDNGIKKVKL 60
           M GVSIV A W AVM   A+ +   GAGIG NWGIM+SHPL PNIVVKLLKDNGIKKVKL
Sbjct: 1   MAGVSIVTAAWGAVMAAVALLAGAAGAGIGVNWGIMASHPLHPNIVVKLLKDNGIKKVKL 60

Query: 61  FDSDSWTVSALSGSKIETVIGIPNDQLKKFAESYDDAKDWVKENVTTHMFEGGVDLRYVS 120
           FDSDSWTVS+LSGSKIETVIGIPNDQLKKFA SYDDAKDWVKENVT HMFEGGVD+RYVS
Sbjct: 61  FDSDSWTVSSLSGSKIETVIGIPNDQLKKFAGSYDDAKDWVKENVTVHMFEGGVDIRYVS 120

Query: 121 VGNEAFLSAYNGTYVKVTYPAMQNIQKALDAAGHGKKVKVTTALNADVYESSTNLPSDGQ 180
           VGNEAFL+AYNG+++  T+PAM NIQKALDAAGHG+KVKVTTALNADVYES +NLPSDGQ
Sbjct: 121 VGNEAFLTAYNGSFLNFTFPAMVNIQKALDAAGHGQKVKVTTALNADVYESRSNLPSDGQ 180

Query: 181 FRPDIYKTMKDIVHFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFFGGGAKATNDKGKSY 240
           FR DI+  MKDIVHFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFFG G K T DKG++Y
Sbjct: 181 FRSDIFSAMKDIVHFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFFGDG-KPTIDKGRTY 240

Query: 241 TNVFDANYDTLIWSLKKIGVTDMKIIVGEVGWPTDGNKFANVELAKRFYDGLFKKLASGN 300
           TNVFDANYDTLIWSLKK+G++D+KIIVGEVGWPTD NKFAN+ LAKRFYDGLFKKLAS  
Sbjct: 241 TNVFDANYDTLIWSLKKVGISDLKIIVGEVGWPTDANKFANLTLAKRFYDGLFKKLASDE 300

Query: 301 GTPMRPKEKFEVYLFGLLDEDMKSVLPGFFERHWGIFQFDGKPKFPMDMSGKGNDKMLVA 360
           GTPMRP EKFEVYLFGLLDEDMKS+LPGFFERHWGIF+FDGKPKFPMD SGKGNDKMLV 
Sbjct: 301 GTPMRPNEKFEVYLFGLLDEDMKSILPGFFERHWGIFRFDGKPKFPMDFSGKGNDKMLVG 360

Query: 361 AKGVQYLEKKWCVLKENVKNFDKISKEVDYACGLSDCTSLGYGSSCNNLDKRGNISYAFN 420
           AKGVQYLE+KWC++K ++KN D+IS EVDYAC  +DCTSL YGSSCN+LD+RGNISYAFN
Sbjct: 361 AKGVQYLERKWCIVKPSLKNLDEISSEVDYACAHADCTSLVYGSSCNHLDRRGNISYAFN 420

Query: 421 MYYQMQDQSVEACDFSGSAHIVKKNASVGSCLFPIQIVSTGERLKAAAAAAVVGLVLGLF 480
           MYYQMQDQSVEAC FSGSA IV KNASVG+CLFPIQIVS GERLK A AAA+VG+VL LF
Sbjct: 421 MYYQMQDQSVEACVFSGSAEIVTKNASVGTCLFPIQIVSAGERLK-AVAAAMVGIVLSLF 480

Query: 481 ALGL 485
           ALGL
Sbjct: 481 ALGL 482

BLAST of Cmc08g0211861 vs. NCBI nr
Match: KAG6595099.1 (Glucan endo-1,3-beta-glucosidase 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 797.0 bits (2057), Expect = 9.3e-227
Identity = 376/484 (77.69%), Postives = 432/484 (89.26%), Query Frame = 0

Query: 1   MTGVSIVPAVWAAVMTVAAVFSVVEGAGIGANWGIMSSHPLRPNIVVKLLKDNGIKKVKL 60
           M G +IVP  WAAV+TVAA+ + V   GIG NWG ++SHPL+P+IVV LLKDNGIKKVKL
Sbjct: 1   MGGATIVPTAWAAVITVAAMAAGVAEGGIGVNWGTLASHPLKPSIVVNLLKDNGIKKVKL 60

Query: 61  FDSDSWTVSALSGSKIETVIGIPNDQLKKFAESYDDAKDWVKENVTTHMFEGGVDLRYVS 120
           FDSD+WTVS+L+GSKIET+IGIPND+LKKFAESY+D K+WVK+NVT HM++GGVD+RYVS
Sbjct: 61  FDSDTWTVSSLAGSKIETIIGIPNDRLKKFAESYNDVKNWVKDNVTVHMYDGGVDIRYVS 120

Query: 121 VGNEAFLSAYNGTYVKVTYPAMQNIQKALDAAGHGKKVKVTTALNADVYESSTNLPSDGQ 180
           VGNEAFL+AYNG++V VT+PAMQN+QKALD AGH KK+KVTTALNADVYE+ +NLPSDGQ
Sbjct: 121 VGNEAFLTAYNGSFVNVTFPAMQNVQKALDEAGHSKKIKVTTALNADVYEAKSNLPSDGQ 180

Query: 181 FRPDIYKTMKDIVHFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFFGGGAKATNDKGKSY 240
           FRPDIY  MK+IV FLDR+ APFMVNIYPFLSLYQNPNFP+DYAFFGG  ++ ND GKSY
Sbjct: 181 FRPDIYSIMKEIVRFLDRHDAPFMVNIYPFLSLYQNPNFPIDYAFFGGSGQSNNDNGKSY 240

Query: 241 TNVFDANYDTLIWSLKKIGVTDMKIIVGEVGWPTDGNKFANVELAKRFYDGLFKKLASGN 300
           TNVFDANYDTLIWSLKK+G  ++KI++GEVGWPTD N FANV LAKRFYDGLF+KLASG 
Sbjct: 241 TNVFDANYDTLIWSLKKVGFPNLKIVIGEVGWPTDANVFANVTLAKRFYDGLFEKLASGE 300

Query: 301 GTPMRPKEKFEVYLFGLLDEDMKSVLPGFFERHWGIFQFDGKPKFPMDMSGKGNDKMLVA 360
           GTPMRPKE FEVYLFGLLDE+ KSVLPGFFERHWGIFQFDGKPKFPMD+SGKGNDKMLVA
Sbjct: 301 GTPMRPKESFEVYLFGLLDENAKSVLPGFFERHWGIFQFDGKPKFPMDLSGKGNDKMLVA 360

Query: 361 AKGVQYLEKKWCVLKENVKNFDKISKEVDYACGLSDCTSLGYGSSCNNLDKRGNISYAFN 420
           AKGVQY+E+KWCVL++ +KN +  +KEVDYACG SDCT+L  G SC  LD+RG ISYAFN
Sbjct: 361 AKGVQYMERKWCVLRQTLKNLEPTAKEVDYACGYSDCTALTAGGSCGQLDRRGRISYAFN 420

Query: 421 MYYQMQDQSVEACDFSGSAHIVKKNASVGSCLFPIQIVSTGERLKAAAAAAVVGLVLGLF 480
           +Y+QMQDQ+VEAC F G+A IV KNA+VG+CLFPIQIVS GERLKAAAAAA+VGLVL LF
Sbjct: 421 IYFQMQDQNVEACVFDGTAEIVTKNATVGNCLFPIQIVSGGERLKAAAAAAMVGLVLSLF 480

Query: 481 ALGL 485
            LGL
Sbjct: 481 VLGL 484

BLAST of Cmc08g0211861 vs. ExPASy Swiss-Prot
Match: Q6NKW9 (Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 PE=2 SV=2)

HSP 1 Score: 528.1 bits (1359), Expect = 1.1e-148
Identity = 256/459 (55.77%), Postives = 329/459 (71.68%), Query Frame = 0

Query: 28  GIGANWGIMSSHPLRPNIVVKLLKDNGIKKVKLFDSDSWTVSALSGSKIETVIGIPNDQL 87
           G+G NWG M++H L P  VV++LKDN I KVKLFD+D  T+ AL+GS +E ++ IPNDQL
Sbjct: 24  GLGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGSGLEVMVAIPNDQL 83

Query: 88  KKFAESYDDAKDWVKENVTTHMFEGGVDLRYVSVGNEAFLSAYNGTYVKVTYPAMQNIQK 147
           K    SYD AKDWV++NVT + F+GGV++ +V+VGNE FL +YNG+++ +T+PA+ NIQ 
Sbjct: 84  KVMT-SYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFINLTFPALANIQN 143

Query: 148 ALDAAGHGKKVKVTTALNADVYES--STNLPSDGQFRPDIYKTMKDIVHFLDRNKAPFMV 207
           AL+ AG G  VK T  LNADVY+S  S  +PS G+FRPDI   M  IV FL +N AP  +
Sbjct: 144 ALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIVDFLGKNNAPITI 203

Query: 208 NIYPFLSLYQNPNFPLDYAFFGGGAKATNDKGKSYTNVFDANYDTLIWSLKKIGVTDMKI 267
           NIYPFLSLY N +FPL+YAFF  GA+  ND G  YTNVFDAN+DTL+ SLK +G  DM I
Sbjct: 204 NIYPFLSLYGNDDFPLNYAFF-DGAEPINDNGIDYTNVFDANFDTLVSSLKAVGHGDMPI 263

Query: 268 IVGEVGWPTDGNKFANVELAKRFYDGLFKKLASGNGTPMRPKEKFEVYLFGLLDEDMKSV 327
           IVGEVGWPT+G+K AN   A RFY+GL  +L +  GTP+RP    EVYLFGLLDED KS+
Sbjct: 264 IVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRP-TYIEVYLFGLLDEDAKSI 323

Query: 328 LPGFFERHWGIFQFDGKPKFPMDMSGKGNDKMLVAAKGVQYLEKKWCVLKENVKNFDKIS 387
            PG FERHWGIF+FDG+PKFP+D+SG+G  K L+ A+ V YL  KWC      K+  K++
Sbjct: 324 APGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCTFNPEAKDLTKLA 383

Query: 388 KEVDYACGLSDCTSLGYGSSCNNLDKRGNISYAFNMYYQMQDQSVEACDFSGSAHIVKKN 447
             +DYAC  SDCT+LGYGSSCN LD  GN SYAFNM++Q+++Q   AC F G A I  +N
Sbjct: 384 ANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESACYFQGLATITTQN 443

Query: 448 ASVGSCLFPIQIVSTGERLKAAAAAAVVGLVLGLFALGL 485
            S G C FPIQIV++     + ++ ++V L++     G+
Sbjct: 444 ISQGQCNFPIQIVASSASSFSCSSYSLVVLIVWFLLSGM 479

BLAST of Cmc08g0211861 vs. ExPASy Swiss-Prot
Match: Q93Z08 (Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 PE=2 SV=2)

HSP 1 Score: 492.7 bits (1267), Expect = 4.9e-138
Identity = 242/449 (53.90%), Postives = 316/449 (70.38%), Query Frame = 0

Query: 11  WAAVMTVAAVFSVVEGA-GIGANWGIMSSHPLRPNIVVKLLKDNGIKKVKLFDSDSWTVS 70
           W +V+ + AV  + + A  IGANWG  +SHPL P+IVV++L++NGI+KVKLFD++  T+ 
Sbjct: 3   WGSVLLLLAVALLCQRASSIGANWGTQASHPLPPDIVVRMLRENGIQKVKLFDAEYDTLR 62

Query: 71  ALSGSKIETVIGIPNDQLKKFAESYDDAKDWVKENVTTHMFEGGVDLRYVSVGNEAFLSA 130
           AL  S IE ++GIPN+ L   A S   A+ WV +NV+TH+    V++RYV+VGNE FLS 
Sbjct: 63  ALGKSGIEVMVGIPNEMLATLASSLKAAEKWVAKNVSTHISTDNVNIRYVAVGNEPFLST 122

Query: 131 YNGTYVKVTYPAMQNIQKALDAAGHGKKVKVTTALNADVYESSTNLPSDGQFRPDIYKTM 190
           YNG+Y+  T+PA++NIQ A+  AG   +VKVT  LNADVY+SST  PS G FR +I   M
Sbjct: 123 YNGSYLSTTFPALRNIQIAIIKAGLQNQVKVTCPLNADVYDSSTTFPSGGDFRANIRDLM 182

Query: 191 KDIVHFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFFGGGAKATNDKGKSYTNVFDANYD 250
             IV FL  N  PF VNIYP++SLY NP+FP+DYAFF G A+  ND G  Y N+FDANYD
Sbjct: 183 ITIVKFLSENGGPFTVNIYPYISLYTNPDFPVDYAFFDGNAQPLNDGGTFYYNMFDANYD 242

Query: 251 TLIWSLKKIGVTDMKIIVGEVGWPTDGNKFANVELAKRFYDGLFKKLASGNGTPMRPKEK 310
           TL+ +L+K G  +M II+GE+GWPTDG+  AN++ AK+F  G    ++ G GTP RP   
Sbjct: 243 TLVHALEKNGFGNMPIIIGEIGWPTDGDSNANLDYAKKFNQGFMAHISGGKGTPRRP-GP 302

Query: 311 FEVYLFGLLDEDMKSVLPGFFERHWGIFQFDGKPKFPMDMSGKGNDKMLVAAKGVQYLEK 370
            + YLF L+DED KSV PG+FERHWGIF FDG PK+ +++ G  N   L+ AKGV+YLE+
Sbjct: 303 IDAYLFSLIDEDAKSVQPGYFERHWGIFTFDGLPKYALNL-GTTNTGALIQAKGVRYLER 362

Query: 371 KWCVLKENVKNFD-KISKEVDYACGLSDCTSLGYGSSCNNLDKRGNISYAFNMYYQMQDQ 430
           KWCV+K NV+  D +++  V YAC L DCTSLG G+SC NLD + NISYAFN YYQ+QDQ
Sbjct: 363 KWCVMKPNVRLDDPQVAPAVSYACSLGDCTSLGVGTSCANLDGKQNISYAFNSYYQIQDQ 422

Query: 431 SVEACDFSGSAHIVKKNASVGSCLFPIQI 458
              AC F   + + K + S G+C FPI I
Sbjct: 423 LDTACKFPNISEVTKTDPSTGTCRFPIMI 449

BLAST of Cmc08g0211861 vs. ExPASy Swiss-Prot
Match: Q9M088 (Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 PE=2 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 1.3e-135
Identity = 239/447 (53.47%), Postives = 309/447 (69.13%), Query Frame = 0

Query: 14  VMTVAAVFSVVEGAGIGANWGIMSSHPLRPNIVVKLLKDNGIKKVKLFDSDSWTVSALSG 73
           V+T+     ++E  GIG NWG  + HPL P  VV+LL++NGI+KVKLF++DS  + ALS 
Sbjct: 12  VITLLYASLLIEVEGIGVNWGSQARHPLPPATVVRLLRENGIQKVKLFEADSAILKALSR 71

Query: 74  SKIETVIGIPNDQLKKFAESYDDAKDWVKENVTTHMFEGGVDLRYVSVGNEAFLSAYNGT 133
           + I+ ++GIPND L   A S   A+ WV +NV+ H+   GVD+RYV+VGNE FL A+NGT
Sbjct: 72  TGIQVMVGIPNDLLAPLAGSVAAAERWVSQNVSAHVSSNGVDIRYVAVGNEPFLKAFNGT 131

Query: 134 YVKVTYPAMQNIQKALDAAGHGKKVKVTTALNADVYESSTNLPSDGQFRPDIYKTMKDIV 193
           +  +T PA+QNIQ A+  AG   +VKVT  LNADVY+S++NLPSDG FRP+I   M +IV
Sbjct: 132 FEGITLPALQNIQSAIIKAGLATQVKVTVPLNADVYQSASNLPSDGDFRPEIRDLMLNIV 191

Query: 194 HFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFFGGGAKATNDKGKSYTNVFDANYDTLIW 253
            FL  N APF +NIYPF+SLY +PNFP+++AFF G     ND G+ Y NV DANYDTL+W
Sbjct: 192 KFLSDNGAPFTINIYPFISLYNDPNFPVEFAFFDGTGTPINDNGRIYDNVLDANYDTLVW 251

Query: 254 SLKKIGVTDMKIIVGEVGWPTDGNKFANVELAKRFYDGLFKKLASGNGTPMRPKEKFEVY 313
           SL+K G  ++ IIVGEVGWPTDG+K AN+  A+R+  G   +  +  GTPMRP    + Y
Sbjct: 252 SLQKNGFGNLTIIVGEVGWPTDGDKNANLMYARRYNQGFMNRQKANKGTPMRP-GAMDAY 311

Query: 314 LFGLLDEDMKSVLPGFFERHWGIFQFDGKPKFPMDMSGKGNDKMLVAAKGVQYLEKKWCV 373
           LFGL+DED KS+ PG FERHWGIF  DG+PK+ + + G GN   L+ AK V YL KKWC+
Sbjct: 312 LFGLIDEDAKSIQPGNFERHWGIFYIDGQPKYQLSL-GSGNG--LIPAKDVHYLAKKWCI 371

Query: 374 LKENVKNFD-KISKEVDYACGLSDCTSLGYGSSCNNLDKRGNISYAFNMYYQMQDQSVEA 433
           L  N    D ++   V YAC  +DCTSLGYGSSC NL+   N+SYAFN YYQ+ +Q   A
Sbjct: 372 LAPNANLQDPQLGPSVSYACDHADCTSLGYGSSCGNLNLAQNVSYAFNSYYQVSNQLDSA 431

Query: 434 CDFSGSAHIVKKNASVGSCLFPIQIVS 460
           C F G + +  ++ SVGSC F I I S
Sbjct: 432 CKFPGLSIVSTRDPSVGSCKFKIMIKS 454

BLAST of Cmc08g0211861 vs. ExPASy Swiss-Prot
Match: Q9FGH4 (Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 PE=2 SV=1)

HSP 1 Score: 347.4 bits (890), Expect = 2.5e-94
Identity = 189/431 (43.85%), Postives = 250/431 (58.00%), Query Frame = 0

Query: 29  IGANWGIMSSHPLRPNIVVKLLKDNGIKKVKLFDSDSWTVSALSGSKIETVIGIPNDQLK 88
           +G NWG  +SHPL P+ VV+LLK NGI KVKLFD+D   + ALSGS I   IGI N  LK
Sbjct: 26  VGINWGTEASHPLPPSKVVELLKSNGIVKVKLFDADPKVLRALSGSNIGVTIGIQNSMLK 85

Query: 89  KFAESYDDAKDWVKENVTTHMFEGG--VDLRYVSVGNEAFLSAYNGTYVKVTYPAMQNIQ 148
               S   A+ WV +NVT + F GG  V + YV+VG E FL +Y   Y      A  NIQ
Sbjct: 86  SLNASVKVAESWVHDNVTRY-FNGGNRVRIEYVAVGEEPFLQSYGNQYKPFVIGAAMNIQ 145

Query: 149 KALDAAGHGKKVKVTTALNADVYESSTNLPSDGQFRPDIYKTMKDIVHFLDRNKAPFMVN 208
            AL  A    +VKV    + D + S +  PS G FR D+ KTM +++ FL ++ +PF V 
Sbjct: 146 NALVKANLANEVKVVVPSSFDSFLSESGRPSSGHFRADLNKTMIELLSFLTKHHSPFFVT 205

Query: 209 IYPFLSLYQNPNFPLDYAFFGGGAKATNDKGKSYTNVFDANYDTLIWSLKKIGVTDMKII 268
           I PFLS +QN N  LD++ F   AKA  D  K+Y N FD +YDTL+ +L  IG +++ I+
Sbjct: 206 ISPFLSFHQNKNISLDFSLFKETAKAHKDGRKTYRNSFDLSYDTLVSALFTIGFSEVDIV 265

Query: 269 VGEVGWPTDGNKFANVELAKRFYDGLFKKLASGNGTPMRPKEKFEVYLFGLLDEDMKSVL 328
           V ++GWPTDG + A    A+ F+ GL   L     T   P+   E Y+  LLDED +++ 
Sbjct: 266 VSKIGWPTDGAENATSLTAEAFFKGLIVHLE--KKTASLPRPPVETYIESLLDEDQRNLS 325

Query: 329 PGFFERHWGIFQFDGKPKFPMDMSGKGNDKMLVAAKGVQYLEKKWCVLKENVKNFDKISK 388
            G FERHWG+F FDG+ K+        N K  V A+ VQYL  KWCV+  N K+    S 
Sbjct: 326 AGNFERHWGVFTFDGQAKYNFSF----NHKNQVNAQNVQYLPPKWCVVNNN-KDLSNASA 385

Query: 389 EVDYACGLSDCTSLGYGSSCNNLDKRGNISYAFNMYYQMQDQSVEACDFSGSAHIVKKNA 448
               AC ++DCTS+  G SC+ +   GN+SYAFN  YQ  D S E+C+F G   I   + 
Sbjct: 386 RALEACAVADCTSILPGGSCSGIRWPGNVSYAFNSLYQQNDHSAESCNFGGLGLITTVDP 445

Query: 449 SVGSCLFPIQI 458
           S  +C F IQ+
Sbjct: 446 SEDNCRFSIQL 448

BLAST of Cmc08g0211861 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 1.6e-56
Identity = 141/435 (32.41%), Postives = 224/435 (51.49%), Query Frame = 0

Query: 29  IGANWGIMSSHPLRPNIVVKLLKDNGIKKVKLFDSDSWTVSALSGSKIETVIGIPNDQLK 88
           +G N+G +S +       V LLK   I+KV+LF ++   + A + + +E VIG  N  + 
Sbjct: 30  LGVNYGQLSDNLPSLQATVNLLKSTTIQKVRLFGAEPAVIKAFANTGVEIVIGFDNGDIP 89

Query: 89  KFAESYDDAKDWVKENVTTHMFEGGVDLRYVSVGNEAFLSAYNGTYVKVTYPAMQNIQKA 148
             A + + A  +VK NV +  F    ++  ++VGNE  L++ +   +    PAMQN+Q A
Sbjct: 90  TLASNPNVASQFVKSNVMS--FYPASNIIAITVGNEV-LTSGDQKLISQLLPAMQNVQNA 149

Query: 149 LDAAGHGKKVKVTTALNADVYESSTNLPSDGQFRPDIYKTMKDIVHFLDRNKAPFMVNIY 208
           L+AA  G KVKV+T ++A    S +  PS G F P +  TMK ++ F   N APFM++ Y
Sbjct: 150 LNAASLGGKVKVST-VHAMAVLSQSYPPSSGVFNPGLGDTMKALLQFQSANDAPFMISPY 209

Query: 209 PFLSLYQNPNFPLDYAFF---GGGAKATNDKGKSYTNVFDANYDTLIWSLKKIGVTDMKI 268
           P+ + Y+N   P   AF        +  +  G  YTN+FDA  D +  +L  +G  D++I
Sbjct: 210 PYFA-YKNQPTPDTLAFCLFQPNAGQVDSGNGHKYTNMFDAQVDAVHSALNAMGFKDIEI 269

Query: 269 IVGEVGWPTDGNK---FANVELAKRFYDGLFKKLASGNGTPMRPKEKFEVYLFGLLDEDM 328
           +V E GWP  G+      +++ AK +   L   L S  GTP+ P +  + YLF L DED 
Sbjct: 270 VVAETGWPHGGDSNEVGPSLDNAKAYVGNLINHLKSKVGTPLMPGKSIDTYLFSLYDEDK 329

Query: 329 KSVLPGFFERHWGIFQFDGKPKFPMD-MSGKGNDKMLVAAKGVQYLEKKWCVLKENVKNF 388
           K+      E+++G+F+ DG   + +  +    N                WCV K  V + 
Sbjct: 330 KTGASS--EKYFGLFKPDGSTTYDVGLLKNTQNPTTPATPTPTPKAAGSWCVPKPGVSD- 389

Query: 389 DKISKEVDYACGLS-DCTSLGYGSSCNNLDK-RGNISYAFNMYYQMQDQSVEACDFSGSA 448
           D+++  ++YACG   DC  +  G +C   +  + + +Y  N+YYQ   ++   CDFS +A
Sbjct: 390 DQLTGNINYACGQGIDCGPIQPGGACFEPNTVKAHAAYVMNLYYQSAGRNSWNCDFSQTA 449

Query: 449 HIVKKNASVGSCLFP 455
            +   N S G+C FP
Sbjct: 450 TLTNTNPSYGACNFP 456

BLAST of Cmc08g0211861 vs. ExPASy TrEMBL
Match: A0A5D3CN37 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G005720 PE=3 SV=1)

HSP 1 Score: 979.9 bits (2532), Expect = 3.7e-282
Identity = 484/484 (100.00%), Postives = 484/484 (100.00%), Query Frame = 0

Query: 1   MTGVSIVPAVWAAVMTVAAVFSVVEGAGIGANWGIMSSHPLRPNIVVKLLKDNGIKKVKL 60
           MTGVSIVPAVWAAVMTVAAVFSVVEGAGIGANWGIMSSHPLRPNIVVKLLKDNGIKKVKL
Sbjct: 1   MTGVSIVPAVWAAVMTVAAVFSVVEGAGIGANWGIMSSHPLRPNIVVKLLKDNGIKKVKL 60

Query: 61  FDSDSWTVSALSGSKIETVIGIPNDQLKKFAESYDDAKDWVKENVTTHMFEGGVDLRYVS 120
           FDSDSWTVSALSGSKIETVIGIPNDQLKKFAESYDDAKDWVKENVTTHMFEGGVDLRYVS
Sbjct: 61  FDSDSWTVSALSGSKIETVIGIPNDQLKKFAESYDDAKDWVKENVTTHMFEGGVDLRYVS 120

Query: 121 VGNEAFLSAYNGTYVKVTYPAMQNIQKALDAAGHGKKVKVTTALNADVYESSTNLPSDGQ 180
           VGNEAFLSAYNGTYVKVTYPAMQNIQKALDAAGHGKKVKVTTALNADVYESSTNLPSDGQ
Sbjct: 121 VGNEAFLSAYNGTYVKVTYPAMQNIQKALDAAGHGKKVKVTTALNADVYESSTNLPSDGQ 180

Query: 181 FRPDIYKTMKDIVHFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFFGGGAKATNDKGKSY 240
           FRPDIYKTMKDIVHFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFFGGGAKATNDKGKSY
Sbjct: 181 FRPDIYKTMKDIVHFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFFGGGAKATNDKGKSY 240

Query: 241 TNVFDANYDTLIWSLKKIGVTDMKIIVGEVGWPTDGNKFANVELAKRFYDGLFKKLASGN 300
           TNVFDANYDTLIWSLKKIGVTDMKIIVGEVGWPTDGNKFANVELAKRFYDGLFKKLASGN
Sbjct: 241 TNVFDANYDTLIWSLKKIGVTDMKIIVGEVGWPTDGNKFANVELAKRFYDGLFKKLASGN 300

Query: 301 GTPMRPKEKFEVYLFGLLDEDMKSVLPGFFERHWGIFQFDGKPKFPMDMSGKGNDKMLVA 360
           GTPMRPKEKFEVYLFGLLDEDMKSVLPGFFERHWGIFQFDGKPKFPMDMSGKGNDKMLVA
Sbjct: 301 GTPMRPKEKFEVYLFGLLDEDMKSVLPGFFERHWGIFQFDGKPKFPMDMSGKGNDKMLVA 360

Query: 361 AKGVQYLEKKWCVLKENVKNFDKISKEVDYACGLSDCTSLGYGSSCNNLDKRGNISYAFN 420
           AKGVQYLEKKWCVLKENVKNFDKISKEVDYACGLSDCTSLGYGSSCNNLDKRGNISYAFN
Sbjct: 361 AKGVQYLEKKWCVLKENVKNFDKISKEVDYACGLSDCTSLGYGSSCNNLDKRGNISYAFN 420

Query: 421 MYYQMQDQSVEACDFSGSAHIVKKNASVGSCLFPIQIVSTGERLKAAAAAAVVGLVLGLF 480
           MYYQMQDQSVEACDFSGSAHIVKKNASVGSCLFPIQIVSTGERLKAAAAAAVVGLVLGLF
Sbjct: 421 MYYQMQDQSVEACDFSGSAHIVKKNASVGSCLFPIQIVSTGERLKAAAAAAVVGLVLGLF 480

Query: 481 ALGL 485
           ALGL
Sbjct: 481 ALGL 484

BLAST of Cmc08g0211861 vs. ExPASy TrEMBL
Match: A0A1S3B101 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103484682 PE=3 SV=1)

HSP 1 Score: 979.9 bits (2532), Expect = 3.7e-282
Identity = 484/484 (100.00%), Postives = 484/484 (100.00%), Query Frame = 0

Query: 1   MTGVSIVPAVWAAVMTVAAVFSVVEGAGIGANWGIMSSHPLRPNIVVKLLKDNGIKKVKL 60
           MTGVSIVPAVWAAVMTVAAVFSVVEGAGIGANWGIMSSHPLRPNIVVKLLKDNGIKKVKL
Sbjct: 1   MTGVSIVPAVWAAVMTVAAVFSVVEGAGIGANWGIMSSHPLRPNIVVKLLKDNGIKKVKL 60

Query: 61  FDSDSWTVSALSGSKIETVIGIPNDQLKKFAESYDDAKDWVKENVTTHMFEGGVDLRYVS 120
           FDSDSWTVSALSGSKIETVIGIPNDQLKKFAESYDDAKDWVKENVTTHMFEGGVDLRYVS
Sbjct: 61  FDSDSWTVSALSGSKIETVIGIPNDQLKKFAESYDDAKDWVKENVTTHMFEGGVDLRYVS 120

Query: 121 VGNEAFLSAYNGTYVKVTYPAMQNIQKALDAAGHGKKVKVTTALNADVYESSTNLPSDGQ 180
           VGNEAFLSAYNGTYVKVTYPAMQNIQKALDAAGHGKKVKVTTALNADVYESSTNLPSDGQ
Sbjct: 121 VGNEAFLSAYNGTYVKVTYPAMQNIQKALDAAGHGKKVKVTTALNADVYESSTNLPSDGQ 180

Query: 181 FRPDIYKTMKDIVHFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFFGGGAKATNDKGKSY 240
           FRPDIYKTMKDIVHFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFFGGGAKATNDKGKSY
Sbjct: 181 FRPDIYKTMKDIVHFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFFGGGAKATNDKGKSY 240

Query: 241 TNVFDANYDTLIWSLKKIGVTDMKIIVGEVGWPTDGNKFANVELAKRFYDGLFKKLASGN 300
           TNVFDANYDTLIWSLKKIGVTDMKIIVGEVGWPTDGNKFANVELAKRFYDGLFKKLASGN
Sbjct: 241 TNVFDANYDTLIWSLKKIGVTDMKIIVGEVGWPTDGNKFANVELAKRFYDGLFKKLASGN 300

Query: 301 GTPMRPKEKFEVYLFGLLDEDMKSVLPGFFERHWGIFQFDGKPKFPMDMSGKGNDKMLVA 360
           GTPMRPKEKFEVYLFGLLDEDMKSVLPGFFERHWGIFQFDGKPKFPMDMSGKGNDKMLVA
Sbjct: 301 GTPMRPKEKFEVYLFGLLDEDMKSVLPGFFERHWGIFQFDGKPKFPMDMSGKGNDKMLVA 360

Query: 361 AKGVQYLEKKWCVLKENVKNFDKISKEVDYACGLSDCTSLGYGSSCNNLDKRGNISYAFN 420
           AKGVQYLEKKWCVLKENVKNFDKISKEVDYACGLSDCTSLGYGSSCNNLDKRGNISYAFN
Sbjct: 361 AKGVQYLEKKWCVLKENVKNFDKISKEVDYACGLSDCTSLGYGSSCNNLDKRGNISYAFN 420

Query: 421 MYYQMQDQSVEACDFSGSAHIVKKNASVGSCLFPIQIVSTGERLKAAAAAAVVGLVLGLF 480
           MYYQMQDQSVEACDFSGSAHIVKKNASVGSCLFPIQIVSTGERLKAAAAAAVVGLVLGLF
Sbjct: 421 MYYQMQDQSVEACDFSGSAHIVKKNASVGSCLFPIQIVSTGERLKAAAAAAVVGLVLGLF 480

Query: 481 ALGL 485
           ALGL
Sbjct: 481 ALGL 484

BLAST of Cmc08g0211861 vs. ExPASy TrEMBL
Match: A0A0A0KJ60 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_6G486900 PE=3 SV=1)

HSP 1 Score: 902.5 bits (2331), Expect = 7.6e-259
Identity = 441/484 (91.12%), Postives = 464/484 (95.87%), Query Frame = 0

Query: 1   MTGVSIVPAVWAAVMTVAAVFSVVEGAGIGANWGIMSSHPLRPNIVVKLLKDNGIKKVKL 60
           M GVS+VPAVWAAVMT+ AVFSVVEGAGIG NWGI+SSHPLRPNIVVKLLKDNGIKKVKL
Sbjct: 1   MAGVSMVPAVWAAVMTLGAVFSVVEGAGIGVNWGILSSHPLRPNIVVKLLKDNGIKKVKL 60

Query: 61  FDSDSWTVSALSGSKIETVIGIPNDQLKKFAESYDDAKDWVKENVTTHMFEGGVDLRYVS 120
           FDSD+WTVS+LSGSKIET+IGIPNDQLKKFA+SYDDAKDWVKENVT+HMFEGGVDLRYVS
Sbjct: 61  FDSDAWTVSSLSGSKIETIIGIPNDQLKKFAKSYDDAKDWVKENVTSHMFEGGVDLRYVS 120

Query: 121 VGNEAFLSAYNGTYVKVTYPAMQNIQKALDAAGHGKKVKVTTALNADVYESSTNLPSDGQ 180
           VGNEAFL+AYNG++VKVT+PAMQNIQKA+DAAGHGKK+KVTTALNADVYESSTNLPSDG+
Sbjct: 121 VGNEAFLTAYNGSFVKVTFPAMQNIQKAIDAAGHGKKIKVTTALNADVYESSTNLPSDGE 180

Query: 181 FRPDIYKTMKDIVHFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFFGGGAKATNDKGKSY 240
           FRPDIY TMKDIVHFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFF GG KATNDK KSY
Sbjct: 181 FRPDIYSTMKDIVHFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFFDGGGKATNDKDKSY 240

Query: 241 TNVFDANYDTLIWSLKKIGVTDMKIIVGEVGWPTDGNKFANVELAKRFYDGLFKKLASGN 300
           TNVFDANYDTLIWSLKK+GV+DMKIIVGEVGWPTDGNKFANV LAKRFYDGLFKKLASG+
Sbjct: 241 TNVFDANYDTLIWSLKKLGVSDMKIIVGEVGWPTDGNKFANVNLAKRFYDGLFKKLASGD 300

Query: 301 GTPMRPKEKFEVYLFGLLDEDMKSVLPGFFERHWGIFQFDGKPKFPMDMSGKGNDKMLVA 360
           GTPMRPKEKFEVYLFGLLDEDMKSVLPGFFERHWGIFQFDGKPKFPMD SGKGNDKMLVA
Sbjct: 301 GTPMRPKEKFEVYLFGLLDEDMKSVLPGFFERHWGIFQFDGKPKFPMDSSGKGNDKMLVA 360

Query: 361 AKGVQYLEKKWCVLKENVKNFDKISKEVDYACGLSDCTSLGYGSSCNNLDKRGNISYAFN 420
           AKGVQYLEKKWCVLK+NVK FD IS +VDYAC LSDCTSLGYGSSCNNLD+RGNISYAFN
Sbjct: 361 AKGVQYLEKKWCVLKKNVKVFDNISPQVDYACSLSDCTSLGYGSSCNNLDRRGNISYAFN 420

Query: 421 MYYQMQDQSVEACDFSGSAHIVKKNASVGSCLFPIQIVSTGERLKAAAAAAVVGLVLGLF 480
           MYYQMQDQSVEAC F+GSA IVK NASVGSCLFPIQIVS GERLK AAAAA+VGLVL LF
Sbjct: 421 MYYQMQDQSVEACVFNGSAEIVKNNASVGSCLFPIQIVSAGERLK-AAAAAMVGLVLSLF 480

Query: 481 ALGL 485
           AL L
Sbjct: 481 ALRL 483

BLAST of Cmc08g0211861 vs. ExPASy TrEMBL
Match: A0A6J1HE69 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111463370 PE=3 SV=1)

HSP 1 Score: 791.2 bits (2042), Expect = 2.5e-225
Identity = 375/484 (77.48%), Postives = 432/484 (89.26%), Query Frame = 0

Query: 1   MTGVSIVPAVWAAVMTVAAVFSVVEGAGIGANWGIMSSHPLRPNIVVKLLKDNGIKKVKL 60
           M G +IVP  WAAV+TVAA+ + V   GIG NWG ++SHPL+P+IVV LLKDNGIKKVKL
Sbjct: 1   MGGATIVPTAWAAVITVAAMAAGVAEGGIGVNWGTLASHPLKPSIVVNLLKDNGIKKVKL 60

Query: 61  FDSDSWTVSALSGSKIETVIGIPNDQLKKFAESYDDAKDWVKENVTTHMFEGGVDLRYVS 120
           FDSD+WTVS+L+GSKIET+IGIPND+LKKFAESY++AK+WVK+NVT HM++GGVD+RYVS
Sbjct: 61  FDSDTWTVSSLAGSKIETIIGIPNDRLKKFAESYNEAKNWVKDNVTVHMYDGGVDIRYVS 120

Query: 121 VGNEAFLSAYNGTYVKVTYPAMQNIQKALDAAGHGKKVKVTTALNADVYESSTNLPSDGQ 180
           VGNEAFL+AYNG++V VT+PAMQN+QKALD AGH KK+KVTTALNADVYE+ +NLPSDGQ
Sbjct: 121 VGNEAFLTAYNGSFVNVTFPAMQNVQKALDEAGHSKKIKVTTALNADVYEAKSNLPSDGQ 180

Query: 181 FRPDIYKTMKDIVHFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFFGGGAKATNDKGKSY 240
           FRPDIY  MK+IV FLDR+ APFMVNIYPFLSLYQNPNFP+DYAFFGG  ++ ND GKSY
Sbjct: 181 FRPDIYSIMKEIVRFLDRHDAPFMVNIYPFLSLYQNPNFPIDYAFFGGSGQSNNDNGKSY 240

Query: 241 TNVFDANYDTLIWSLKKIGVTDMKIIVGEVGWPTDGNKFANVELAKRFYDGLFKKLASGN 300
           TNVFDANYDTLIWSLKK+G  ++KI++GEVGWPTD N FANV LAKRFYDGLF+KLASG 
Sbjct: 241 TNVFDANYDTLIWSLKKVGFPNLKIVIGEVGWPTDANVFANVTLAKRFYDGLFEKLASGE 300

Query: 301 GTPMRPKEKFEVYLFGLLDEDMKSVLPGFFERHWGIFQFDGKPKFPMDMSGKGNDKMLVA 360
           GTPMRPKE FEVYLFGLLDE+ KSVLPGFFERHWGIFQFDGKPKFPMD+SGKGNDKMLVA
Sbjct: 301 GTPMRPKESFEVYLFGLLDENAKSVLPGFFERHWGIFQFDGKPKFPMDLSGKGNDKMLVA 360

Query: 361 AKGVQYLEKKWCVLKENVKNFDKISKEVDYACGLSDCTSLGYGSSCNNLDKRGNISYAFN 420
           AKGVQY+E+KWCVL++ +KN +  +KEVDYACG SDCT+L  G SC  LD+RG ISYAFN
Sbjct: 361 AKGVQYMERKWCVLRQTLKNLEPTAKEVDYACGYSDCTALTAGGSCGQLDRRGRISYAFN 420

Query: 421 MYYQMQDQSVEACDFSGSAHIVKKNASVGSCLFPIQIVSTGERLKAAAAAAVVGLVLGLF 480
           +Y+QMQDQ+VEAC F G+A IV KNA+VG+CLFPIQIVS GERLK AAAAA+VGLVL LF
Sbjct: 421 IYFQMQDQNVEACVFDGTAEIVTKNATVGNCLFPIQIVSGGERLK-AAAAAMVGLVLSLF 480

Query: 481 ALGL 485
            LGL
Sbjct: 481 VLGL 483

BLAST of Cmc08g0211861 vs. ExPASy TrEMBL
Match: A0A6J1KUX5 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111497444 PE=3 SV=1)

HSP 1 Score: 788.9 bits (2036), Expect = 1.2e-224
Identity = 372/484 (76.86%), Postives = 433/484 (89.46%), Query Frame = 0

Query: 1   MTGVSIVPAVWAAVMTVAAVFSVVEGAGIGANWGIMSSHPLRPNIVVKLLKDNGIKKVKL 60
           M G +IVP VWAAV+TVAA+ + V   G+G NWG ++SHPL+P+IVV LLKDNGIKKVKL
Sbjct: 1   MGGAAIVPTVWAAVITVAAMAAGVAEGGVGVNWGTLASHPLKPSIVVNLLKDNGIKKVKL 60

Query: 61  FDSDSWTVSALSGSKIETVIGIPNDQLKKFAESYDDAKDWVKENVTTHMFEGGVDLRYVS 120
           FDSD+WTVS+L+GSKIETVIGIPND+LKKFAESY++AK+WVK+NVT HM++GG+D+RYVS
Sbjct: 61  FDSDTWTVSSLAGSKIETVIGIPNDRLKKFAESYNEAKNWVKDNVTVHMYDGGIDIRYVS 120

Query: 121 VGNEAFLSAYNGTYVKVTYPAMQNIQKALDAAGHGKKVKVTTALNADVYESSTNLPSDGQ 180
           VGNEAFL+AYNG++V VT+PAMQN+QKALD AGHGKK+KVTTALNADVYE+ +NLPSDGQ
Sbjct: 121 VGNEAFLTAYNGSFVNVTFPAMQNVQKALDEAGHGKKIKVTTALNADVYEAKSNLPSDGQ 180

Query: 181 FRPDIYKTMKDIVHFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFFGGGAKATNDKGKSY 240
           FRPDIY  +K+IV FLDR+ APFMVNIYPFLSLYQNPNFP+DYAFFGG  ++ +D GKSY
Sbjct: 181 FRPDIYSIIKEIVRFLDRHDAPFMVNIYPFLSLYQNPNFPIDYAFFGGSGQSNDDNGKSY 240

Query: 241 TNVFDANYDTLIWSLKKIGVTDMKIIVGEVGWPTDGNKFANVELAKRFYDGLFKKLASGN 300
           TNVFDANYDTLIWSLKKIG  ++KI++GEVGWPTD N FANV LAKRFYDGLF+KLASG 
Sbjct: 241 TNVFDANYDTLIWSLKKIGFPNLKIVIGEVGWPTDANVFANVTLAKRFYDGLFEKLASGE 300

Query: 301 GTPMRPKEKFEVYLFGLLDEDMKSVLPGFFERHWGIFQFDGKPKFPMDMSGKGNDKMLVA 360
           GTPMRPKE FEVYLFGLLDE+ KSVLPGFFERHWGIFQFDGKPKFPMD+SGKGNDKMLVA
Sbjct: 301 GTPMRPKESFEVYLFGLLDENAKSVLPGFFERHWGIFQFDGKPKFPMDLSGKGNDKMLVA 360

Query: 361 AKGVQYLEKKWCVLKENVKNFDKISKEVDYACGLSDCTSLGYGSSCNNLDKRGNISYAFN 420
           AKGVQY+E+KWCVL++ +KN D  ++EVDYAC  SDCT+L  G SC+ LD+RG ISYAFN
Sbjct: 361 AKGVQYMERKWCVLRQTLKNLDPTAREVDYACSYSDCTALTAGGSCSQLDRRGRISYAFN 420

Query: 421 MYYQMQDQSVEACDFSGSAHIVKKNASVGSCLFPIQIVSTGERLKAAAAAAVVGLVLGLF 480
           +Y+QMQDQ+VEAC F G+A IV KNA++G+CLFPIQIVS GERLK AAA A+VGLVL LF
Sbjct: 421 IYFQMQDQNVEACVFGGTAEIVTKNATIGNCLFPIQIVSAGERLK-AAAVAMVGLVLSLF 480

Query: 481 ALGL 485
            LGL
Sbjct: 481 VLGL 483

BLAST of Cmc08g0211861 vs. TAIR 10
Match: AT5G64790.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 577.0 bits (1486), Expect = 1.4e-164
Identity = 277/475 (58.32%), Postives = 361/475 (76.00%), Query Frame = 0

Query: 10  VWAAVMTVAAVFSVVEGAGIGANWGIMSSHPLRPNIVVKLLKDNGIKKVKLFDSDSWTVS 69
           V   +MTV  + S+V   G G NWG ++SHPL PNIVV++LKDN I KVKLFD+DSWT++
Sbjct: 11  VGTVIMTVLTLASLV--GGFGVNWGNIASHPLNPNIVVQMLKDNKINKVKLFDADSWTMN 70

Query: 70  ALSGSKIETVIGIPNDQLKKFAESYDDAKDWVKENVTTHMFEGGVDLRYVSVGNEAFLSA 129
           AL+G+ +E ++GIPN+ L+  A+ YD+AKDWVKENVT ++ +GGVD++YV+VGNE FLSA
Sbjct: 71  ALAGTGMEVMVGIPNNLLESLADDYDNAKDWVKENVTQYIRKGGVDIKYVAVGNEPFLSA 130

Query: 130 YNGTYVKVTYPAMQNIQKALDAAGHGKKVKVTTALNADVYESSTNLPSDGQFRPDIYKTM 189
           YNG+++K T+PA++NI KAL  AGH   +K T   NA+VY+S+ + PS+G FR D+ +TM
Sbjct: 131 YNGSFLKTTFPALKNIHKALKEAGHTNIMKATIPQNAEVYQSANDKPSEGDFRKDVKQTM 190

Query: 190 KDIVHFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFFGGGAKATNDKGKSYTNVFDANYD 249
            DIV+F   N  PF VNIYPFLSLY N +FP+++AF  G  +   DKGK+Y NVFDANYD
Sbjct: 191 LDIVNFFHENDLPFTVNIYPFLSLYLNEHFPVEFAFLDGDGQTMTDKGKNYDNVFDANYD 250

Query: 250 TLIWSLKKIGVTDMKIIVGEVGWPTDGNKFANVELAKRFYDGLFKKLASGNGTPMRPKEK 309
           TL+++LKK G+ DMKIIVGEVGWPTDG+K+A+ +LA++FY GL K+LA   GTP RP E+
Sbjct: 251 TLVYALKKAGIHDMKIIVGEVGWPTDGHKYASPKLAEKFYAGLMKRLAKDGGTPTRP-ER 310

Query: 310 FEVYLFGLLDEDMKSVLPGFFERHWGIFQFDGKPKFPMDMSGKGNDKMLVAAKGVQYLEK 369
            EVYLFG LDEDMKS+LPG FERHWGIF++DG PKF +D +G+G   + VAAKGVQYLEK
Sbjct: 311 LEVYLFGFLDEDMKSILPGPFERHWGIFRYDGTPKFMLDFTGQGRQMVPVAAKGVQYLEK 370

Query: 370 KWCVLKENVKNFDKISKEVDYACGLSDCTSLGYGSSCNNLDKRGNISYAFNMYYQMQDQS 429
           +WCV+ ++  N D++  ++DYAC   DCT++  GS+C+ L K  NISYAFNMY+Q+QDQ 
Sbjct: 371 QWCVVNKDTVNLDEVGPDLDYACYHGDCTAMEAGSTCSKLTKVQNISYAFNMYFQIQDQD 430

Query: 430 VEACDFSGSAHIVKKNASVGSCLFPIQIVSTGERLKAAAAAAVVGLVLGLFALGL 485
           V ACDF G+A I K NASVGSCLFP+QIVS  +  +         +V GL  LGL
Sbjct: 431 VRACDFKGAAMITKVNASVGSCLFPVQIVSGSDDFRINFVFGRF-VVFGLVLLGL 481

BLAST of Cmc08g0211861 vs. TAIR 10
Match: AT2G19440.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 531.9 bits (1369), Expect = 5.2e-151
Identity = 262/465 (56.34%), Postives = 337/465 (72.47%), Query Frame = 0

Query: 20  VFSVVEGAGIGANWGIMSSHPLRPNIVVKLLKDNGIKKVKLFDSDSWTVSALSGSKIETV 79
           +  +V   G+G NWG M++H L P  VV++LKDN I KVKLFD+D  T+SALSGS +E +
Sbjct: 12  IMGIVMVDGLGVNWGTMATHKLPPKKVVQMLKDNNINKVKLFDADETTMSALSGSGLEVM 71

Query: 80  IGIPNDQLKKFAESYDDAKDWVKENVTTHMFEGGVDLRYVSVGNEAFLSAYNGTYVKVTY 139
           + IPNDQLK    SYD AKDWV +NVT + F GGV++ +V+VGNE FL +YNG+++ +T+
Sbjct: 72  VAIPNDQLKVMG-SYDRAKDWVHKNVTRYNFNGGVNITFVAVGNEPFLKSYNGSFINLTF 131

Query: 140 PAMQNIQKALDAAGHGKKVKVTTALNADVYE--SSTNLPSDGQFRPDIYKTMKDIVHFLD 199
           PA+QNIQ AL+ AG G  VK T  LNADVY+  SS  +PS G+FRPDI   M  IV FL 
Sbjct: 132 PALQNIQNALNEAGLGSSVKATVPLNADVYDSPSSNPVPSAGRFRPDIIGQMTQIVDFLG 191

Query: 200 RNKAPFMVNIYPFLSLYQNPNFPLDYAFFGGGAKATNDKGKSYTNVFDANYDTLIWSLKK 259
            N AP  +NIYPFLSLY N +FPL+YAFF  GAK  +D G +YTNVFDAN+DTL+ +LK 
Sbjct: 192 NNSAPITINIYPFLSLYGNDDFPLNYAFF-DGAKPVDDNGIAYTNVFDANFDTLVSALKA 251

Query: 260 IGVTDMKIIVGEVGWPTDGNKFANVELAKRFYDGLFKKLASGNGTPMRPKEKFEVYLFGL 319
           +G  DM IIVGEVGWPT+G+K AN   A RFY+GL  +L    GTP+RP    EVYLFGL
Sbjct: 252 VGHGDMPIIVGEVGWPTEGDKHANSGSAYRFYNGLLPRLGENRGTPLRP-TYIEVYLFGL 311

Query: 320 LDEDMKSVLPGFFERHWGIFQFDGKPKFPMDMSGKGNDKMLVAAKGVQYLEKKWCVLKEN 379
           LDED KS+ PG FERHWGIF+FDG+PKFP+D+SG+G +K+L+ A+ V Y  KKWC+    
Sbjct: 312 LDEDAKSIAPGEFERHWGIFKFDGQPKFPIDLSGQGQNKLLIGAENVTYQPKKWCMFNTE 371

Query: 380 VKNFDKISKEVDYACGLSDCTSLGYGSSCNNLDKRGNISYAFNMYYQMQDQSVEACDFSG 439
            K+  K++  +DYAC  SDCT+LGYGSSCN LD  GN SYAFNMY+Q+++Q  +AC F G
Sbjct: 372 AKDLTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMYFQVKNQDEDACIFQG 431

Query: 440 SAHIVKKNASVGSCLFPIQIVSTGERLKAAAAAAVVGLVLGLFAL 483
            A I  KN S G C FPIQIV++     ++ ++++V L+ G++ L
Sbjct: 432 LATITTKNISQGQCNFPIQIVAS---TASSFSSSLVLLIAGVWFL 470

BLAST of Cmc08g0211861 vs. TAIR 10
Match: AT1G64760.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 528.1 bits (1359), Expect = 7.5e-150
Identity = 256/459 (55.77%), Postives = 329/459 (71.68%), Query Frame = 0

Query: 28  GIGANWGIMSSHPLRPNIVVKLLKDNGIKKVKLFDSDSWTVSALSGSKIETVIGIPNDQL 87
           G+G NWG M++H L P  VV++LKDN I KVKLFD+D  T+ AL+GS +E ++ IPNDQL
Sbjct: 24  GLGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGSGLEVMVAIPNDQL 83

Query: 88  KKFAESYDDAKDWVKENVTTHMFEGGVDLRYVSVGNEAFLSAYNGTYVKVTYPAMQNIQK 147
           K    SYD AKDWV++NVT + F+GGV++ +V+VGNE FL +YNG+++ +T+PA+ NIQ 
Sbjct: 84  KVMT-SYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFINLTFPALANIQN 143

Query: 148 ALDAAGHGKKVKVTTALNADVYES--STNLPSDGQFRPDIYKTMKDIVHFLDRNKAPFMV 207
           AL+ AG G  VK T  LNADVY+S  S  +PS G+FRPDI   M  IV FL +N AP  +
Sbjct: 144 ALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIVDFLGKNNAPITI 203

Query: 208 NIYPFLSLYQNPNFPLDYAFFGGGAKATNDKGKSYTNVFDANYDTLIWSLKKIGVTDMKI 267
           NIYPFLSLY N +FPL+YAFF  GA+  ND G  YTNVFDAN+DTL+ SLK +G  DM I
Sbjct: 204 NIYPFLSLYGNDDFPLNYAFF-DGAEPINDNGIDYTNVFDANFDTLVSSLKAVGHGDMPI 263

Query: 268 IVGEVGWPTDGNKFANVELAKRFYDGLFKKLASGNGTPMRPKEKFEVYLFGLLDEDMKSV 327
           IVGEVGWPT+G+K AN   A RFY+GL  +L +  GTP+RP    EVYLFGLLDED KS+
Sbjct: 264 IVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRP-TYIEVYLFGLLDEDAKSI 323

Query: 328 LPGFFERHWGIFQFDGKPKFPMDMSGKGNDKMLVAAKGVQYLEKKWCVLKENVKNFDKIS 387
            PG FERHWGIF+FDG+PKFP+D+SG+G  K L+ A+ V YL  KWC      K+  K++
Sbjct: 324 APGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCTFNPEAKDLTKLA 383

Query: 388 KEVDYACGLSDCTSLGYGSSCNNLDKRGNISYAFNMYYQMQDQSVEACDFSGSAHIVKKN 447
             +DYAC  SDCT+LGYGSSCN LD  GN SYAFNM++Q+++Q   AC F G A I  +N
Sbjct: 384 ANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESACYFQGLATITTQN 443

Query: 448 ASVGSCLFPIQIVSTGERLKAAAAAAVVGLVLGLFALGL 485
            S G C FPIQIV++     + ++ ++V L++     G+
Sbjct: 444 ISQGQCNFPIQIVASSASSFSCSSYSLVVLIVWFLLSGM 479

BLAST of Cmc08g0211861 vs. TAIR 10
Match: AT1G64760.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 528.1 bits (1359), Expect = 7.5e-150
Identity = 256/459 (55.77%), Postives = 329/459 (71.68%), Query Frame = 0

Query: 28  GIGANWGIMSSHPLRPNIVVKLLKDNGIKKVKLFDSDSWTVSALSGSKIETVIGIPNDQL 87
           G+G NWG M++H L P  VV++LKDN I KVKLFD+D  T+ AL+GS +E ++ IPNDQL
Sbjct: 24  GLGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGSGLEVMVAIPNDQL 83

Query: 88  KKFAESYDDAKDWVKENVTTHMFEGGVDLRYVSVGNEAFLSAYNGTYVKVTYPAMQNIQK 147
           K    SYD AKDWV++NVT + F+GGV++ +V+VGNE FL +YNG+++ +T+PA+ NIQ 
Sbjct: 84  KVMT-SYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFINLTFPALANIQN 143

Query: 148 ALDAAGHGKKVKVTTALNADVYES--STNLPSDGQFRPDIYKTMKDIVHFLDRNKAPFMV 207
           AL+ AG G  VK T  LNADVY+S  S  +PS G+FRPDI   M  IV FL +N AP  +
Sbjct: 144 ALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIVDFLGKNNAPITI 203

Query: 208 NIYPFLSLYQNPNFPLDYAFFGGGAKATNDKGKSYTNVFDANYDTLIWSLKKIGVTDMKI 267
           NIYPFLSLY N +FPL+YAFF  GA+  ND G  YTNVFDAN+DTL+ SLK +G  DM I
Sbjct: 204 NIYPFLSLYGNDDFPLNYAFF-DGAEPINDNGIDYTNVFDANFDTLVSSLKAVGHGDMPI 263

Query: 268 IVGEVGWPTDGNKFANVELAKRFYDGLFKKLASGNGTPMRPKEKFEVYLFGLLDEDMKSV 327
           IVGEVGWPT+G+K AN   A RFY+GL  +L +  GTP+RP    EVYLFGLLDED KS+
Sbjct: 264 IVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRP-TYIEVYLFGLLDEDAKSI 323

Query: 328 LPGFFERHWGIFQFDGKPKFPMDMSGKGNDKMLVAAKGVQYLEKKWCVLKENVKNFDKIS 387
            PG FERHWGIF+FDG+PKFP+D+SG+G  K L+ A+ V YL  KWC      K+  K++
Sbjct: 324 APGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCTFNPEAKDLTKLA 383

Query: 388 KEVDYACGLSDCTSLGYGSSCNNLDKRGNISYAFNMYYQMQDQSVEACDFSGSAHIVKKN 447
             +DYAC  SDCT+LGYGSSCN LD  GN SYAFNM++Q+++Q   AC F G A I  +N
Sbjct: 384 ANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESACYFQGLATITTQN 443

Query: 448 ASVGSCLFPIQIVSTGERLKAAAAAAVVGLVLGLFALGL 485
            S G C FPIQIV++     + ++ ++V L++     G+
Sbjct: 444 ISQGQCNFPIQIVASSASSFSCSSYSLVVLIVWFLLSGM 479

BLAST of Cmc08g0211861 vs. TAIR 10
Match: AT5G18220.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 513.1 bits (1320), Expect = 2.5e-145
Identity = 257/454 (56.61%), Postives = 322/454 (70.93%), Query Frame = 0

Query: 12  AAVMTVAAVFSVVEG--AGIGANWGIMSSHPLRPNIVVKLLKDNGIKKVKLFDSDSWTVS 71
           A +     + S++ G  + +G NWG MS+H L P  VV++LKDN +KKVKLFD+D+ T+ 
Sbjct: 11  AGIFLCITIVSLLSGDVSALGVNWGTMSTHQLPPKTVVQMLKDNNVKKVKLFDADTNTMV 70

Query: 72  ALSGSKIETVIGIPNDQLKKFAESYDDAKDWVKENVTTHMFEGGVDLRYVSVGNEAFLSA 131
           AL+GS IE ++ IPNDQLK    SY+ AKDWV+ N+T   F   V ++YV+VGNE FL+A
Sbjct: 71  ALAGSGIEVMVAIPNDQLKAMG-SYNRAKDWVRRNIT--RFNDDVKIKYVAVGNEPFLTA 130

Query: 132 YNGTYVKVTYPAMQNIQKALDAAGHGKKVKVTTALNADVYES--STNLPSDGQFRPDIYK 191
           YNG+++ +TYPA+ NIQKAL+ AG G  +K T  LNADVY S     +PS G FR DI++
Sbjct: 131 YNGSFINLTYPALFNIQKALNEAGVGDFIKATVPLNADVYNSPLENPVPSAGSFRQDIFE 190

Query: 192 TMKDIVHFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFFGGGAKATNDKGKSYTNVFDAN 251
            MK IV+FL  NKAPF VNIYPFLSLY + +FP DYAFF  G    +D G  YTNVFDAN
Sbjct: 191 EMKLIVNFLAHNKAPFTVNIYPFLSLYLSSDFPFDYAFF-NGQNTVSDNGVIYTNVFDAN 250

Query: 252 YDTLIWSLKKIGVTDMKIIVGEVGWPTDGNKFANVELAKRFYDGLFKKLASGNGTPMRPK 311
           +DTL+ SLK +G  DM +IVGEVGWPTDG+K AN+  A+RFY GL  KLA+  GTPMRP 
Sbjct: 251 FDTLLASLKALGHGDMTVIVGEVGWPTDGDKNANIPNAERFYTGLLPKLAANRGTPMRP- 310

Query: 312 EKFEVYLFGLLDEDMKSVLPGFFERHWGIFQFDGKPKFPMDMSGKGNDKMLVAAKGVQYL 371
              EVYLFG +DED KS+ PG FERHWGIF++DG+PKFP D+SG G  K+L  A+ VQYL
Sbjct: 311 GYIEVYLFGFIDEDAKSIAPGNFERHWGIFKYDGQPKFPADLSGAGQKKILTGAQNVQYL 370

Query: 372 EKKWCVLKENV----KNFDKISKEVDYACGLSDCTSLGYGSSCNNLDKRGNISYAFNMYY 431
             +WC+   N      N +++   V+YAC  SDCT+LGYGSSC NLD+ GN SYAFNMY+
Sbjct: 371 RNQWCMFNPNALTFSNNTNQLGDNVNYACTFSDCTALGYGSSCGNLDEVGNASYAFNMYF 430

Query: 432 QMQDQSVEACDFSGSAHIVKKNASVGSCLFPIQI 458
           Q+Q+Q  EACDF G A I  +N S   C FPIQI
Sbjct: 431 QVQNQKAEACDFEGLAIITTRNISREQCNFPIQI 459

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008440124.17.7e-282100.00PREDICTED: glucan endo-1,3-beta-glucosidase 8 [Cucumis melo][more]
TYK12975.17.7e-282100.00tRNA(His) guanylyltransferase 2-like [Cucumis melo var. makuwa][more]
XP_004142041.11.6e-25891.12glucan endo-1,3-beta-glucosidase 8 [Cucumis sativus] >KGN48417.1 hypothetical pr... [more]
XP_038883272.12.2e-23683.47glucan endo-1,3-beta-glucosidase 8 [Benincasa hispida][more]
KAG6595099.19.3e-22777.69Glucan endo-1,3-beta-glucosidase 8, partial [Cucurbita argyrosperma subsp. soror... [more]
Match NameE-valueIdentityDescription
Q6NKW91.1e-14855.77Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 ... [more]
Q93Z084.9e-13853.90Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 ... [more]
Q9M0881.3e-13553.47Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 ... [more]
Q9FGH42.5e-9443.85Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 ... [more]
Q94G861.6e-5632.41Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3CN373.7e-282100.00(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3B1013.7e-282100.00(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103484682 PE=3 SV... [more]
A0A0A0KJ607.6e-25991.12(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_6G486900 PE=3... [more]
A0A6J1HE692.5e-22577.48(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111463370 P... [more]
A0A6J1KUX51.2e-22476.86(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111497444 PE=... [more]
Match NameE-valueIdentityDescription
AT5G64790.11.4e-16458.32O-Glycosyl hydrolases family 17 protein [more]
AT2G19440.15.2e-15156.34O-Glycosyl hydrolases family 17 protein [more]
AT1G64760.17.5e-15055.77O-Glycosyl hydrolases family 17 protein [more]
AT1G64760.27.5e-15055.77O-Glycosyl hydrolases family 17 protein [more]
AT5G18220.12.5e-14556.61O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 370..453
e-value: 1.0E-37
score: 141.3
IPR012946X8 domainPFAMPF07983X8coord: 370..439
e-value: 3.6E-18
score: 66.0
NoneNo IPR availableGENE3D1.20.58.1040coord: 366..461
e-value: 3.3E-9
score: 39.0
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 28..356
e-value: 2.2E-110
score: 370.6
NoneNo IPR availablePANTHERPTHR32227:SF290BETA-1,3-GLUCANASEcoord: 14..467
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 29..348
e-value: 1.4E-68
score: 231.8
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 263..276
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 14..467
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 29..347

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc08g0211861.1Cmc08g0211861.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0099116 tRNA 5'-end processing
biological_process GO:0006400 tRNA modification
cellular_component GO:0016020 membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0005525 GTP binding
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0008193 tRNA guanylyltransferase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds