Cmc06g0175491 (gene) Melon (Charmono) v1.1

Overview
NameCmc06g0175491
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionSubtilisin-like protease SBT5.3
LocationCMiso1.1chr06: 32366509 .. 32369434 (-)
RNA-Seq ExpressionCmc06g0175491
SyntenyCmc06g0175491
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTTTGTTGTCCTTAATGATCTTCAACTATCGACACCAGGGTGCAAGTCTTTCTGAAAAAGGATTGCCAATTCAGAAGTTCTATCCATTGATCAATTCTGTAGATGCAAAAGTCCAAAATTTCTCAAACAAATACGCGTGAGTATATCTTCGTTTAAATTATATAAAATGTTCGATCCCTTAAGTAGGTTTCAATCGTATTCTTAAACGTTGAAAACAATTATTAGTAAATGTTTGGGTGTGATTTTGAAATTATTAAATCATTTTTATCACGTACAAAATCAACTTAATATTAAATTTTACACTTCCAAATGCAATTTTAATTTGAACGTTGTTACTGCTAAGTATGCATGCCTTTCAAATTTTACAATCATTTTCATATTAACACAGGAAAAAAAAAACAAACCCTTAACCGGTACTTTTCATCGCAATTAAGATGAAACTTTTCAAAGTGATCTAAAAAGACGTTATATATAACCGAAACCTAAGATATAAAGTTCAAAGTCTTTTCATGGTAAAAAGAAGAAAAAGAACCAAAATGAGATTTAAGAGCAAATTTGAATGTGTTGTTATAATTATTGTAGGCAACTATGTGAAATAGAGTCTCTTGATCCTGAAAAGGTAAAAGGGAAGATTGTAGTTTGCCTTAGAGGAGACAATGCAAGAACGGAAAAAGGTTATGTGGTTGCTAAAGCAGGTGGTGTTGGAATGATTCTTGCTAATGCTGAGGAAAATGGGGATGACATTTCGGCTGATGCACATTTGCTTCCTGCTTCTCATATTACCTATTCTGATGGTCAATTAGTCTACCAATACATCAACTCTACCAAGTAAACTTTACTATCTTCTTCTTCTATCAACTCCTATCTTGGTTAAATTAATTATTATATGTCTAAGTTCGTGTCTATTTACTCACTCAAACTTTTAAAAACGTTATTCGACATTTATGTGTGTCTTATAAGTTTTTTAACATTTTATAATATAAACACAAGAATTGAAAGTTCAAGGGGGTCACTATTAGATAAATCAATTAGTTCAAAAAAAATTAAATATATCAAGAATCTTAATTGGACATAAAATTTAATGTTTCAAGAACTTACTGGACAATTTTCATTAATCATTTAGTTTTTTCGGTTTTGATTTTTGAAATTTAACCTCATAACACTTCTACCACATGTCTGTTCTTTTGTTTTGGTTATTAACTTTTAGGATTTTTTTTCAAATTAAAAACCAGAGACAAGTTTTGAAAACTGAAAAGACAGTACTTTCTGGGCCGTATTTGATAATCATTTGGTTTGTAGTTTTTAACTTTTGAAAATAATGTTTCTTTTTCCACATTTTCGTCATCATAGTTTTCATCTTTTCTAATTTAGTAAGCAAACAAATGCTCAACTAAGTTCTTAAAAAGACTACTTATTTTAGCTTTCAAAACTTAACATGAATTTTGAAAACAGTCCTAATTACATGTAAACAAAATAGAACCTTAGAGGTCCGTTAAACATCCTTTTTTTTCATCTCCAAAGATACATTAGACACAAACTTAAAATATCGAATACGACTAAAGCACTCGTAGAACTCATACGACACATTTTATACTTTGAACAAGAATTCCAATGGCTTACCTGACGCACCCAAGGACAGAGTCAGGAGTCAAACCAGCACCGATTATGGCTTCATTCTCGTCAAGGGGTCCCAACTCAATCGATCCATCAATACTCAAGGTTAGGTCAAAATGTTATGAATCACTTATCTAACACTCTAAATGATTAAAGCATCGTTATGCTCTATCTTATATCTAATATACTAAATCTTATGTGTTTGGTTAGTTTTCTTGTATGATTTTTTACTTATCGATCTTTTAGATAAATGTACAAAATTCAATAACAAGATCCAAAGAAATCATAAAATTTTTGTTTATAATATTTTCAGCCAGATATAACAGCACCAGGTGAGAATATATTAGCAGCTTATAGTGAAGATGGATCACCATCAGGGCTATATTTCGATAAACGTAGAGTGCCATTTAGTGTTGAATCTGGGACTTCTATGTCATGCCCTCATGTTTCTGGAATTGTTGGTCTTCTCAAAACACTTTATCCTAAATGGAGTCCTGCTGCTATTAGATCTGCTATCATGACCACAGGTACTCTCTTTTGTTTTTTAGTTGAGCTATATGTTCATTTTGGCTTACATCGATATTTTAAACGTTGGATGTTTACATAATTTTGCATTAAGAAACATTTGACTTAAATCTTGGCAGCTGGAACAAAAGCCAATGACTTGAATCCAATACTAAGCACAAAGCAAGAGAAAGCAAATGAATGGGCATATGGTGCAGGCCATGTTCGTCCAAACAAAGCAGCAGATCCAGGCCTTGTTTATGATCTTTCCACTGAAGACTATTTGAATTACTTATGTGCTCTAGGCTACAAGATAAAACAAATCAAACAATTCTCGAATGACACTTCATTCGTTTGTTCAAAATCATTCAAAATAATCGATCTCAACTACCCGTCGATCTCGATTCCAAATTTGGAGTCAGATGTTGCTTTGAAGATCAAAAGGAGACTGAAGAATGTGGGAAGTCCAGGCACATATGTTGTTCAAGTCAATGCGCCATTAGGGGTTTCGGTTTCGGTTGAGCCAACTAGTTTGAAGTTTACTGGAATTGATGAAGAGAAGAGTTTTCGAGTTGTGTTGAAAAGTAACGTGCCTAATGGTTTTCATCCAGAGTATGTGTTTGGGAAACTAGAATGGTCCGATGGGAATAACCATCGTGTTAGAAGTCCAATTGTAGTGAAATTAGGTAAATGATCATTTGGGTTGAGTTGGATCAAGTCATGATCGGACAAGTTTCGGTTGTGCTTAGCACATTCTTTTGGAAAATTCTTCTCTTGTTCACAGTGTGTTGTTAATTATGTACT

mRNA sequence

ATGTTTTTGTTGTCCTTAATGATCTTCAACTATCGACACCAGGGTGCAAGTCTTTCTGAAAAAGGATTGCCAATTCAGAAGTTCTATCCATTGATCAATTCTGTAGATGCAAAAGTCCAAAATTTCTCAAACAAATACGCGCAACTATGTGAAATAGAGTCTCTTGATCCTGAAAAGGTAAAAGGGAAGATTGTAGTTTGCCTTAGAGGAGACAATGCAAGAACGGAAAAAGGTTATGTGGTTGCTAAAGCAGGTGGTGTTGGAATGATTCTTGCTAATGCTGAGGAAAATGGGGATGACATTTCGGCTGATGCACATTTGCTTCCTGCTTCTCATATTACCTATTCTGATGGTCAATTAGTCTACCAATACATCAACTCTACCAAAATTCCAATGGCTTACCTGACGCACCCAAGGACAGAGTCAGGAGTCAAACCAGCACCGATTATGGCTTCATTCTCGTCAAGGGGTCCCAACTCAATCGATCCATCAATACTCAAGCCAGATATAACAGCACCAGGTGAGAATATATTAGCAGCTTATAGTGAAGATGGATCACCATCAGGGCTATATTTCGATAAACGTAGAGTGCCATTTAGTGTTGAATCTGGGACTTCTATGTCATGCCCTCATGTTTCTGGAATTGTTGGTCTTCTCAAAACACTTTATCCTAAATGGAGTCCTGCTGCTATTAGATCTGCTATCATGACCACAGCTGGAACAAAAGCCAATGACTTGAATCCAATACTAAGCACAAAGCAAGAGAAAGCAAATGAATGGGCATATGGTGCAGGCCATGTTCGTCCAAACAAAGCAGCAGATCCAGGCCTTGTTTATGATCTTTCCACTGAAGACTATTTGAATTACTTATGTGCTCTAGGCTACAAGATAAAACAAATCAAACAATTCTCGAATGACACTTCATTCGTTTGTTCAAAATCATTCAAAATAATCGATCTCAACTACCCGTCGATCTCGATTCCAAATTTGGAGTCAGATGTTGCTTTGAAGATCAAAAGGAGACTGAAGAATGTGGGAAGTCCAGGCACATATGTTGTTCAAGTCAATGCGCCATTAGGGGTTTCGGTTTCGGTTGAGCCAACTAGTTTGAAGTTTACTGGAATTGATGAAGAGAAGAGTTTTCGAGTTGTGTTGAAAAGTAACGTGCCTAATGGTTTTCATCCAGAGTATGTGTTTGGGAAACTAGAATGGTCCGATGGGAATAACCATCGTGTTAGAAGTCCAATTGTAGTGAAATTAGGTAAATGATCATTTGGGTTGAGTTGGATCAAGTCATGATCGGACAAGTTTCGGTTGTGCTTAGCACATTCTTTTGGAAAATTCTTCTCTTGTTCACAGTGTGTTGTTAATTATGTACT

Coding sequence (CDS)

ATGTTTTTGTTGTCCTTAATGATCTTCAACTATCGACACCAGGGTGCAAGTCTTTCTGAAAAAGGATTGCCAATTCAGAAGTTCTATCCATTGATCAATTCTGTAGATGCAAAAGTCCAAAATTTCTCAAACAAATACGCGCAACTATGTGAAATAGAGTCTCTTGATCCTGAAAAGGTAAAAGGGAAGATTGTAGTTTGCCTTAGAGGAGACAATGCAAGAACGGAAAAAGGTTATGTGGTTGCTAAAGCAGGTGGTGTTGGAATGATTCTTGCTAATGCTGAGGAAAATGGGGATGACATTTCGGCTGATGCACATTTGCTTCCTGCTTCTCATATTACCTATTCTGATGGTCAATTAGTCTACCAATACATCAACTCTACCAAAATTCCAATGGCTTACCTGACGCACCCAAGGACAGAGTCAGGAGTCAAACCAGCACCGATTATGGCTTCATTCTCGTCAAGGGGTCCCAACTCAATCGATCCATCAATACTCAAGCCAGATATAACAGCACCAGGTGAGAATATATTAGCAGCTTATAGTGAAGATGGATCACCATCAGGGCTATATTTCGATAAACGTAGAGTGCCATTTAGTGTTGAATCTGGGACTTCTATGTCATGCCCTCATGTTTCTGGAATTGTTGGTCTTCTCAAAACACTTTATCCTAAATGGAGTCCTGCTGCTATTAGATCTGCTATCATGACCACAGCTGGAACAAAAGCCAATGACTTGAATCCAATACTAAGCACAAAGCAAGAGAAAGCAAATGAATGGGCATATGGTGCAGGCCATGTTCGTCCAAACAAAGCAGCAGATCCAGGCCTTGTTTATGATCTTTCCACTGAAGACTATTTGAATTACTTATGTGCTCTAGGCTACAAGATAAAACAAATCAAACAATTCTCGAATGACACTTCATTCGTTTGTTCAAAATCATTCAAAATAATCGATCTCAACTACCCGTCGATCTCGATTCCAAATTTGGAGTCAGATGTTGCTTTGAAGATCAAAAGGAGACTGAAGAATGTGGGAAGTCCAGGCACATATGTTGTTCAAGTCAATGCGCCATTAGGGGTTTCGGTTTCGGTTGAGCCAACTAGTTTGAAGTTTACTGGAATTGATGAAGAGAAGAGTTTTCGAGTTGTGTTGAAAAGTAACGTGCCTAATGGTTTTCATCCAGAGTATGTGTTTGGGAAACTAGAATGGTCCGATGGGAATAACCATCGTGTTAGAAGTCCAATTGTAGTGAAATTAGGTAAATGA

Protein sequence

MFLLSLMIFNYRHQGASLSEKGLPIQKFYPLINSVDAKVQNFSNKYAQLCEIESLDPEKVKGKIVVCLRGDNARTEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYSDGQLVYQYINSTKIPMAYLTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGENILAAYSEDGSPSGLYFDKRRVPFSVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLNPILSTKQEKANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYKIKQIKQFSNDTSFVCSKSFKIIDLNYPSISIPNLESDVALKIKRRLKNVGSPGTYVVQVNAPLGVSVSVEPTSLKFTGIDEEKSFRVVLKSNVPNGFHPEYVFGKLEWSDGNNHRVRSPIVVKLGK
Homology
BLAST of Cmc06g0175491 vs. NCBI nr
Match: TYK19382.1 (subtilisin-like protease SBT5.3 [Cucumis melo var. makuwa])

HSP 1 Score: 820.5 bits (2118), Expect = 6.8e-234
Identity = 409/411 (99.51%), Postives = 410/411 (99.76%), Query Frame = 0

Query: 12  RH-QGASLSEKGLPIQKFYPLINSVDAKVQNFSNKYAQLCEIESLDPEKVKGKIVVCLRG 71
           RH +GASLSEKGLPIQKFYPLINSVDAKVQNFSNKYAQLCEIESLDPEKVKGKIVVCLRG
Sbjct: 400 RHFKGASLSEKGLPIQKFYPLINSVDAKVQNFSNKYAQLCEIESLDPEKVKGKIVVCLRG 459

Query: 72  DNARTEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYSDGQLVYQYINSTKI 131
           DNARTEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYSDGQLVYQYINSTKI
Sbjct: 460 DNARTEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYSDGQLVYQYINSTKI 519

Query: 132 PMAYLTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGENILAAYSEDGSPSGL 191
           PMAYLTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGENILAAYSEDGSPSGL
Sbjct: 520 PMAYLTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGENILAAYSEDGSPSGL 579

Query: 192 YFDKRRVPFSVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLNPIL 251
           YFDKRRVPFSVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLNPIL
Sbjct: 580 YFDKRRVPFSVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLNPIL 639

Query: 252 STKQEKANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYKIKQIKQFSNDTSFV 311
           STKQEKANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYKIKQIKQFSNDTSFV
Sbjct: 640 STKQEKANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYKIKQIKQFSNDTSFV 699

Query: 312 CSKSFKIIDLNYPSISIPNLESDVALKIKRRLKNVGSPGTYVVQVNAPLGVSVSVEPTSL 371
           CSKSFKIIDLNYPSISIPNLESDVALKIKRRLKNVGSPGTYVVQVNAPLGVSVSVEPTSL
Sbjct: 700 CSKSFKIIDLNYPSISIPNLESDVALKIKRRLKNVGSPGTYVVQVNAPLGVSVSVEPTSL 759

Query: 372 KFTGIDEEKSFRVVLKSNVPNGFHPEYVFGKLEWSDGNNHRVRSPIVVKLG 422
           KFTGIDEEKSFRVVLKSNVPNGFHPEYVFGKLEWSDGNNHRVRSPIVVKLG
Sbjct: 760 KFTGIDEEKSFRVVLKSNVPNGFHPEYVFGKLEWSDGNNHRVRSPIVVKLG 810

BLAST of Cmc06g0175491 vs. NCBI nr
Match: XP_016900594.1 (PREDICTED: subtilisin-like protease SBT5.3, partial [Cucumis melo])

HSP 1 Score: 818.9 bits (2114), Expect = 2.0e-233
Identity = 407/412 (98.79%), Postives = 410/412 (99.51%), Query Frame = 0

Query: 12   RH-QGASLSEKGLPIQKFYPLINSVDAKVQNFSNKYAQLCEIESLDPEKVKGKIVVCLRG 71
            RH +GASLSEKGLP+QKFYPLINSVDAKVQNFSNKYAQLCEIESLDPEKVKGKIVVCLRG
Sbjct: 1094 RHFKGASLSEKGLPVQKFYPLINSVDAKVQNFSNKYAQLCEIESLDPEKVKGKIVVCLRG 1153

Query: 72   DNARTEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYSDGQLVYQYINSTKI 131
            DNARTEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYSDGQLVYQYINSTKI
Sbjct: 1154 DNARTEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYSDGQLVYQYINSTKI 1213

Query: 132  PMAYLTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGENILAAYSEDGSPSGL 191
            PMAYLTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGENILAAYSEDGSPSGL
Sbjct: 1214 PMAYLTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGENILAAYSEDGSPSGL 1273

Query: 192  YFDKRRVPFSVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLNPIL 251
            YFDKRRVPF+VESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLNPIL
Sbjct: 1274 YFDKRRVPFNVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLNPIL 1333

Query: 252  STKQEKANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYKIKQIKQFSNDTSFV 311
            STKQEKANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYKIKQIKQFSNDTSFV
Sbjct: 1334 STKQEKANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYKIKQIKQFSNDTSFV 1393

Query: 312  CSKSFKIIDLNYPSISIPNLESDVALKIKRRLKNVGSPGTYVVQVNAPLGVSVSVEPTSL 371
            CSKSFKI DLNYPSISIPNLESDVALKIKRRLKNVGSPGTYVVQVNAPLGVSVSVEPTSL
Sbjct: 1394 CSKSFKITDLNYPSISIPNLESDVALKIKRRLKNVGSPGTYVVQVNAPLGVSVSVEPTSL 1453

Query: 372  KFTGIDEEKSFRVVLKSNVPNGFHPEYVFGKLEWSDGNNHRVRSPIVVKLGK 423
            KFTGIDEEKSFRVVLKSNVPNGFHPEYVFGKLEWSDGNNHRVRSPIVVKLGK
Sbjct: 1454 KFTGIDEEKSFRVVLKSNVPNGFHPEYVFGKLEWSDGNNHRVRSPIVVKLGK 1505

BLAST of Cmc06g0175491 vs. NCBI nr
Match: KAE8650095.1 (hypothetical protein Csa_011146 [Cucumis sativus])

HSP 1 Score: 688.0 bits (1774), Expect = 5.3e-194
Identity = 342/411 (83.21%), Postives = 371/411 (90.27%), Query Frame = 0

Query: 12   RH-QGASLSEKGLPIQKFYPLINSVDAKVQNFSNKYAQLCEIESLDPEKVKGKIVVCLRG 71
            RH +GAS+S+K LP+QKFYPLI+S+DAK +N ++  A LCE  SLDP+KV GKI++CLRG
Sbjct: 1793 RHFKGASVSDKKLPVQKFYPLISSLDAKAKNVTDNDALLCEEGSLDPKKVNGKIIICLRG 1852

Query: 72   DNARTEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYSDGQLVYQYINSTKI 131
            DNAR  KGYV AKAG VGMILANAEENGD+I ADAHLLPASHITYSDGQLVYQYINSTKI
Sbjct: 1853 DNARVAKGYVAAKAGAVGMILANAEENGDEILADAHLLPASHITYSDGQLVYQYINSTKI 1912

Query: 132  PMAYLTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGENILAAYSEDGSPSGL 191
            PMAY+TH RTE G+KPAP+MASFSSRGPN++DPSILKPDITAPGENILAAYS D SP+G 
Sbjct: 1913 PMAYMTHVRTEEGIKPAPVMASFSSRGPNTVDPSILKPDITAPGENILAAYSRDASPTGT 1972

Query: 192  YFDKRRVPFSVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLNPIL 251
             FDKRRVPF+VESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDL PIL
Sbjct: 1973 DFDKRRVPFNVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLTPIL 2032

Query: 252  STKQEKANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYKIKQIKQFSNDTSFV 311
            ST QEKAN +AYGAGHV PN+AADPGLVYDLST+DYLNYLCA GY   QIKQFSNDTSFV
Sbjct: 2033 STNQEKANAFAYGAGHVSPNRAADPGLVYDLSTKDYLNYLCAQGYNTAQIKQFSNDTSFV 2092

Query: 312  CSKSFKIIDLNYPSISIPNLESDVALKIKRRLKNVGSPGTYVVQVNAPLGVSVSVEPTSL 371
            CSKSFK+ DLNYPSISIP LE DVA+KIKR+LKNVGSPGTYVVQV  PLGVSVSVEPTSL
Sbjct: 2093 CSKSFKLTDLNYPSISIPILEYDVAVKIKRKLKNVGSPGTYVVQVKEPLGVSVSVEPTSL 2152

Query: 372  KFTGIDEEKSFRVVLKSNVPNGFHPEYVFGKLEWSDGNNHRVRSPIVVKLG 422
            KFTGIDEEKSFRVVLKS  PNG  P+Y+FGKLEWSDG  HRVRSPIVV+LG
Sbjct: 2153 KFTGIDEEKSFRVVLKSYEPNGSGPKYLFGKLEWSDG-KHRVRSPIVVRLG 2202

BLAST of Cmc06g0175491 vs. NCBI nr
Match: XP_011650462.2 (subtilisin-like protease SBT5.4 [Cucumis sativus])

HSP 1 Score: 688.0 bits (1774), Expect = 5.3e-194
Identity = 342/411 (83.21%), Postives = 371/411 (90.27%), Query Frame = 0

Query: 12  RH-QGASLSEKGLPIQKFYPLINSVDAKVQNFSNKYAQLCEIESLDPEKVKGKIVVCLRG 71
           RH +GAS+S+K LP+QKFYPLI+S+DAK +N ++  A LCE  SLDP+KV GKI++CLRG
Sbjct: 355 RHFKGASVSDKKLPVQKFYPLISSLDAKAKNVTDNDALLCEEGSLDPKKVNGKIIICLRG 414

Query: 72  DNARTEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYSDGQLVYQYINSTKI 131
           DNAR  KGYV AKAG VGMILANAEENGD+I ADAHLLPASHITYSDGQLVYQYINSTKI
Sbjct: 415 DNARVAKGYVAAKAGAVGMILANAEENGDEILADAHLLPASHITYSDGQLVYQYINSTKI 474

Query: 132 PMAYLTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGENILAAYSEDGSPSGL 191
           PMAY+TH RTE G+KPAP+MASFSSRGPN++DPSILKPDITAPGENILAAYS D SP+G 
Sbjct: 475 PMAYMTHVRTEEGIKPAPVMASFSSRGPNTVDPSILKPDITAPGENILAAYSRDASPTGT 534

Query: 192 YFDKRRVPFSVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLNPIL 251
            FDKRRVPF+VESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDL PIL
Sbjct: 535 DFDKRRVPFNVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLTPIL 594

Query: 252 STKQEKANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYKIKQIKQFSNDTSFV 311
           ST QEKAN +AYGAGHV PN+AADPGLVYDLST+DYLNYLCA GY   QIKQFSNDTSFV
Sbjct: 595 STNQEKANAFAYGAGHVSPNRAADPGLVYDLSTKDYLNYLCAQGYNTAQIKQFSNDTSFV 654

Query: 312 CSKSFKIIDLNYPSISIPNLESDVALKIKRRLKNVGSPGTYVVQVNAPLGVSVSVEPTSL 371
           CSKSFK+ DLNYPSISIP LE DVA+KIKR+LKNVGSPGTYVVQV  PLGVSVSVEPTSL
Sbjct: 655 CSKSFKLTDLNYPSISIPILEYDVAVKIKRKLKNVGSPGTYVVQVKEPLGVSVSVEPTSL 714

Query: 372 KFTGIDEEKSFRVVLKSNVPNGFHPEYVFGKLEWSDGNNHRVRSPIVVKLG 422
           KFTGIDEEKSFRVVLKS  PNG  P+Y+FGKLEWSDG  HRVRSPIVV+LG
Sbjct: 715 KFTGIDEEKSFRVVLKSYEPNGSGPKYLFGKLEWSDG-KHRVRSPIVVRLG 764

BLAST of Cmc06g0175491 vs. NCBI nr
Match: XP_038904154.1 (subtilisin-like protease SBT5.4 [Benincasa hispida])

HSP 1 Score: 682.9 bits (1761), Expect = 1.7e-192
Identity = 341/408 (83.58%), Postives = 371/408 (90.93%), Query Frame = 0

Query: 14  QGASLSEKGLPIQKFYPLINSVDAKVQNFSNKYAQLCEIESLDPEKVKGKIVVCLRGDNA 73
           +GASLS+K LP QKFYPLI+S+DAK  N SN +A +CE  SLDP+KVKGKIVVCLRG NA
Sbjct: 366 KGASLSDKILPEQKFYPLISSLDAKANNVSNYHALICEEGSLDPKKVKGKIVVCLRGINA 425

Query: 74  RTEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYSDGQLVYQYINSTKIPMA 133
           R +KG+V A+AG VGMILAN +ENGD++ ADAHLLPASHITYSDGQLVYQYINSTKIPMA
Sbjct: 426 RVDKGFVAAEAGAVGMILANDKENGDELLADAHLLPASHITYSDGQLVYQYINSTKIPMA 485

Query: 134 YLTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGENILAAYSEDGSPSGLYFD 193
           YLTH RTE GVKPAP+MASFSSRGPN+IDPSILKPDITAPG NILAAYSED SPSG  FD
Sbjct: 486 YLTHVRTELGVKPAPVMASFSSRGPNTIDPSILKPDITAPGVNILAAYSEDASPSGSSFD 545

Query: 194 KRRVPFSVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLNPILSTK 253
           KRRV F+VESGTSMSCPH+SGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLNPILSTK
Sbjct: 546 KRRVAFNVESGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLNPILSTK 605

Query: 254 QEKANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYKIKQIKQFSNDTSFVCSK 313
           QEKAN +AYGAGHVRPNKAA+PGLVYDLST+DYLNYLCALGY   QIKQFSNDTSFVCSK
Sbjct: 606 QEKANAFAYGAGHVRPNKAANPGLVYDLSTKDYLNYLCALGYNKTQIKQFSNDTSFVCSK 665

Query: 314 SFKIIDLNYPSISIPNLESDVALKIKRRLKNVGSPGTYVVQVNAPLGVSVSVEPTSLKFT 373
           SFK  DLNYPSISIP+L+S+  +KIKRRLKNVGSPGTYVVQVNAP GVSV VEPTSLKFT
Sbjct: 666 SFKTTDLNYPSISIPDLKSEAVVKIKRRLKNVGSPGTYVVQVNAPPGVSVFVEPTSLKFT 725

Query: 374 GIDEEKSFRVVLKSNVPNGFHPEYVFGKLEWSDGNNHRVRSPIVVKLG 422
           GIDEEKSFRVVLKS+VPN F   YVFG++EWSDG NHRV+SPIVV++G
Sbjct: 726 GIDEEKSFRVVLKSSVPNDFRQMYVFGRIEWSDG-NHRVKSPIVVRVG 772

BLAST of Cmc06g0175491 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 1.5e-122
Identity = 230/423 (54.37%), Postives = 296/423 (69.98%), Query Frame = 0

Query: 2   FLLSLMIFNYRH-QGASLSEKGLPIQKFYPLINSVDAKVQNFSNKYAQLCEIESLDPEKV 61
           F  +L++ N +H +G SLS   LP  KFYP++ SV+AK +N S   AQLC++ SLDP K 
Sbjct: 356 FASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKT 415

Query: 62  KGKIVVCLRGDNARTEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYSDGQL 121
           KGKI+VCLRG N R EKG  VA  GG+GM+L N    G+D+ AD H+LPA+ +T  D   
Sbjct: 416 KGKILVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFA 475

Query: 122 VYQYINSTKIPMAYLTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGENILAA 181
           V +YI+ TK P+A++T  RT+ G+KPAP+MASFSS+GP+ + P ILKPDITAPG +++AA
Sbjct: 476 VSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAA 535

Query: 182 YSEDGSPSGLYFDKRRVPFSVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAG 241
           Y+   SP+   FD RR+ F+  SGTSMSCPH+SGI GLLKT YP WSPAAIRSAIMTTA 
Sbjct: 536 YTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTAT 595

Query: 242 TKANDLNPILSTKQEKANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYKIKQI 301
              +   PI +    KA  +++GAGHV+PN A +PGLVYDL  +DYLN+LC+LGY   QI
Sbjct: 596 IMDDIPGPIQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQI 655

Query: 302 KQFSNDTSFVCSKSFKIIDLNYPSISIPNLESDVALKIKRRLKNVGSPGTYVVQVNAPLG 361
             FS +     S    +++LNYPSI++PNL S   + + R +KNVG P  Y V+VN P G
Sbjct: 656 SVFSGNNFTCSSPKISLVNLNYPSITVPNLTSS-KVTVSRTVKNVGRPSMYTVKVNNPQG 715

Query: 362 VSVSVEPTSLKFTGIDEEKSFRVVL---KSNVPNGFHPEYVFGKLEWSDGNNHRVRSPIV 421
           V V+V+PTSL FT + E+K+F+V+L   K NV  G    YVFG+L WSD   HRVRSPIV
Sbjct: 716 VYVAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKG----YVFGELVWSD-KKHRVRSPIV 772

BLAST of Cmc06g0175491 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 9.6e-122
Identity = 223/409 (54.52%), Postives = 289/409 (70.66%), Query Frame = 0

Query: 14  QGASLSEKGLPIQKFYPLINSVDAKVQNFSNKYAQLCEIESLDPEKVKGKIVVCLRGDNA 73
           +G SLS K LP +K Y LI++ DA V N +   A LC+  SLDP+KVKGKI+VCLRGDNA
Sbjct: 376 KGTSLS-KPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNA 435

Query: 74  RTEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYSDGQLVYQYINSTKIPMA 133
           R +KG   A AG  GM+L N + +G++I +DAH+LPAS I Y DG+ ++ Y++STK P  
Sbjct: 436 RVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKG 495

Query: 134 YLTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGENILAAYSEDGSPSGLYFD 193
           Y+  P      KPAP MASFSSRGPN+I P ILKPDITAPG NI+AA++E   P+ L  D
Sbjct: 496 YIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSD 555

Query: 194 KRRVPFSVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLNPILSTK 253
            RR PF+ ESGTSMSCPH+SG+VGLLKTL+P WSPAAIRSAIMTT+ T+ N   P++   
Sbjct: 556 NRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDES 615

Query: 254 QEKANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYKIKQIKQFSNDTSFVCSK 313
            +KAN ++YG+GHV+PNKAA PGLVYDL+T DYL++LCA+GY    ++ F+ D  + C +
Sbjct: 616 FKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQ 675

Query: 314 SFKIIDLNYPSISIPNLESDVALKIKRRLKNVGSPGTYVVQVNAPLGVSVSVEPTSLKFT 373
              ++D NYPSI++PNL   +   + R+LKNVG P TY  +   PLGV VSVEP  L F 
Sbjct: 676 GANLLDFNYPSITVPNLTGSIT--VTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFN 735

Query: 374 GIDEEKSFRVVLK--SNVPNGFHPEYVFGKLEWSDGNNHRVRSPIVVKL 421
              E K F++ L+     P+G    YVFG+L W+D ++H VRSPIVV+L
Sbjct: 736 KTGEVKIFQMTLRPLPVTPSG----YVFGELTWTD-SHHYVRSPIVVQL 776

BLAST of Cmc06g0175491 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 4.5e-95
Identity = 199/410 (48.54%), Postives = 266/410 (64.88%), Query Frame = 0

Query: 15  GASLSEKGLPIQKFYPLINSVDAKVQNFSNKYAQLCEIESLDPEKVKGKIVVCLRGDNAR 74
           G SL +      K  P I + +A   N +N    LC   +L PEKVKGKIV+C RG NAR
Sbjct: 355 GVSLFKGEALPDKLLPFIYAGNA--SNATN--GNLCMTGTLIPEKVKGKIVMCDRGINAR 414

Query: 75  TEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYSDGQLVYQYINSTKIPMAY 134
            +KG VV  AGGVGMILAN   NG+++ ADAHLLPA+ +    G ++  Y+ +   P A 
Sbjct: 415 VQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTAS 474

Query: 135 LTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGENILAAYSEDGSPSGLYFDK 194
           ++   T  GVKP+P++A+FSSRGPNSI P+ILKPD+ APG NILAA++    P+GL  D 
Sbjct: 475 ISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDS 534

Query: 195 RRVPFSVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLNPILSTKQ 254
           RRV F++ SGTSMSCPHVSG+  LLK+++P+WSPAAIRSA+MTTA     D  P+L    
Sbjct: 535 RRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIAT 594

Query: 255 EK-ANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYKIKQIKQFSNDTSFVC-- 314
            K +  + +GAGHV P  A +PGL+YDL+TEDYL +LCAL Y   QI+  S   ++ C  
Sbjct: 595 GKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSR-RNYTCDP 654

Query: 315 SKSFKIIDLNYPSISIPNLESDVALKIKRRLKNVGSPGTYVVQVNA-PLGVSVSVEPTSL 374
           SKS+ + DLNYPS ++ N++   A K  R + +VG  GTY V+V +   GV +SVEP  L
Sbjct: 655 SKSYSVADLNYPSFAV-NVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVL 714

Query: 375 KFTGIDEEKSFRV--VLKSNVPNGFHPEYVFGKLEWSDGNNHRVRSPIVV 419
            F   +E+KS+ V   + S+ P+G +    FG +EWSDG  H V SP+ +
Sbjct: 715 NFKEANEKKSYTVTFTVDSSKPSGSNS---FGSIEWSDG-KHVVGSPVAI 754

BLAST of Cmc06g0175491 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 341.3 bits (874), Expect = 1.6e-92
Identity = 206/431 (47.80%), Postives = 260/431 (60.32%), Query Frame = 0

Query: 2   FLLSLMIFNYRHQGASLSEKGLPIQKFYPLINSVDAKVQNFSNKYAQLCEIESLDPEKVK 61
           F  +L I N   +GASL    LP  + + LI S DAK+ N + + AQLC   +LD  KV 
Sbjct: 361 FSSNLTINNQLIEGASLF-VNLPPNQAFSLILSTDAKLANATFRDAQLCRRGTLDRTKVN 420

Query: 62  GKIVVCLR-GDNARTEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYSDGQL 121
           GKIV+C R G      +G     AG  GMIL N  +NG  +SA+ H+    +      + 
Sbjct: 421 GKIVLCTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKS 480

Query: 122 VYQYINSTKI----------PMAYLTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDI 181
               + +T I              ++  RT  G KPAP+MASFSSRGPN I PSILKPD+
Sbjct: 481 RPHGVKTTAIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPDV 540

Query: 182 TAPGENILAAYSEDGSPSGLYFDKRR-VPFSVESGTSMSCPHVSGIVGLLKTLYPKWSPA 241
           TAPG NILAAYSE  S S L  D RR   F+V  GTSMSCPH SGI GLLKT +P WSPA
Sbjct: 541 TAPGVNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPA 600

Query: 242 AIRSAIMTTAGTKANDLNPILST-KQEKANEWAYGAGHVRPNKAADPGLVYDLSTEDYLN 301
           AI+SAIMTTA T  N   PI     +  A+ +AYG+GHVRP+ A +PGLVYDLS  DYLN
Sbjct: 601 AIKSAIMTTATTLDNTNRPIQDAFDKTLADAFAYGSGHVRPDLAIEPGLVYDLSLTDYLN 660

Query: 302 YLCALGYKIKQIKQFSNDTSFVCSKSFKIIDLNYPSISIPNLESDVALKIKRRLKNVGSP 361
           +LCA GY  + I   + + +F+CS S  + DLNYPSI++PNL     + I R + NVG P
Sbjct: 661 FLCASGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRLK-PVTIARTVTNVGPP 720

Query: 362 GTYVVQVNAPLGVSVSVEPTSLKFTGIDEEKSFRVVLKSNVPNGFHPEYVFGKLEWSDGN 420
            TY V   +P G S++V P SL FT I E K+F+V+++++       +Y FG L W+DG 
Sbjct: 721 STYTVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQAS-SAATRRKYEFGDLRWTDG- 780

BLAST of Cmc06g0175491 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 1.8e-83
Identity = 169/373 (45.31%), Postives = 228/373 (61.13%), Query Frame = 0

Query: 47  AQLCEIESLDPEKVKGKIVVCLRGDNARTEKGYVVAKAGGVGMILANAEENGDDISADAH 106
           + LC   SLD   V+GKIVVC RG NAR EKG VV  AGG+GMI+AN   +G+++ AD+H
Sbjct: 377 SNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSH 436

Query: 107 LLPASHITYSDGQLVYQYINSTKIPMAYLTHPRTESGVKPAPIMASFSSRGPNSIDPSIL 166
           LLPA  +    G L+ +Y+ S   P A L    T   VKP+P++A+FSSRGPN++ P IL
Sbjct: 437 LLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEIL 496

Query: 167 KPDITAPGENILAAYSEDGSPSGLYFDKRRVPFSVESGTSMSCPHVSGIVGLLKTLYPKW 226
           KPD+  PG NILA +S+   P+GL  D RR  F++ SGTSMSCPH+SG+ GLLK  +P+W
Sbjct: 497 KPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEW 556

Query: 227 SPAAIRSAIMTTAGTKANDLNPILSTKQEK-ANEWAYGAGHVRPNKAADPGLVYDLSTED 286
           SP+AI+SA+MTTA    N   P+        +N +A+G+GHV P KA  PGLVYD+STE+
Sbjct: 557 SPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEE 616

Query: 287 YLNYLCALGYKIKQIKQFSNDTSFVCSKSFKII-DLNYPSISIPNLESDVALKIKRRLKN 346
           Y+ +LC+L Y +  I       S  CSK F     LNYPS S+        ++  R + N
Sbjct: 617 YIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSV-LFGGKRVVRYTREVTN 676

Query: 347 VGSPGT-YVVQVNAPLGVSVSVEPTSLKFTGIDEEKSFRVVLKSNVPNGFHPEYVFGKLE 406
           VG+  + Y V VN    V +SV+P+ L F  + E+K + V   S        +  FG + 
Sbjct: 677 VGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSIT 736

Query: 407 WSDGNNHRVRSPI 417
           WS+   H VRSP+
Sbjct: 737 WSN-PQHEVRSPV 747

BLAST of Cmc06g0175491 vs. ExPASy TrEMBL
Match: A0A5D3D773 (Subtilisin-like protease SBT5.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00130 PE=3 SV=1)

HSP 1 Score: 820.5 bits (2118), Expect = 3.3e-234
Identity = 409/411 (99.51%), Postives = 410/411 (99.76%), Query Frame = 0

Query: 12  RH-QGASLSEKGLPIQKFYPLINSVDAKVQNFSNKYAQLCEIESLDPEKVKGKIVVCLRG 71
           RH +GASLSEKGLPIQKFYPLINSVDAKVQNFSNKYAQLCEIESLDPEKVKGKIVVCLRG
Sbjct: 400 RHFKGASLSEKGLPIQKFYPLINSVDAKVQNFSNKYAQLCEIESLDPEKVKGKIVVCLRG 459

Query: 72  DNARTEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYSDGQLVYQYINSTKI 131
           DNARTEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYSDGQLVYQYINSTKI
Sbjct: 460 DNARTEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYSDGQLVYQYINSTKI 519

Query: 132 PMAYLTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGENILAAYSEDGSPSGL 191
           PMAYLTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGENILAAYSEDGSPSGL
Sbjct: 520 PMAYLTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGENILAAYSEDGSPSGL 579

Query: 192 YFDKRRVPFSVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLNPIL 251
           YFDKRRVPFSVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLNPIL
Sbjct: 580 YFDKRRVPFSVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLNPIL 639

Query: 252 STKQEKANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYKIKQIKQFSNDTSFV 311
           STKQEKANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYKIKQIKQFSNDTSFV
Sbjct: 640 STKQEKANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYKIKQIKQFSNDTSFV 699

Query: 312 CSKSFKIIDLNYPSISIPNLESDVALKIKRRLKNVGSPGTYVVQVNAPLGVSVSVEPTSL 371
           CSKSFKIIDLNYPSISIPNLESDVALKIKRRLKNVGSPGTYVVQVNAPLGVSVSVEPTSL
Sbjct: 700 CSKSFKIIDLNYPSISIPNLESDVALKIKRRLKNVGSPGTYVVQVNAPLGVSVSVEPTSL 759

Query: 372 KFTGIDEEKSFRVVLKSNVPNGFHPEYVFGKLEWSDGNNHRVRSPIVVKLG 422
           KFTGIDEEKSFRVVLKSNVPNGFHPEYVFGKLEWSDGNNHRVRSPIVVKLG
Sbjct: 760 KFTGIDEEKSFRVVLKSNVPNGFHPEYVFGKLEWSDGNNHRVRSPIVVKLG 810

BLAST of Cmc06g0175491 vs. ExPASy TrEMBL
Match: A0A1S4DX85 (subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491136 PE=3 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 9.6e-234
Identity = 407/412 (98.79%), Postives = 410/412 (99.51%), Query Frame = 0

Query: 12   RH-QGASLSEKGLPIQKFYPLINSVDAKVQNFSNKYAQLCEIESLDPEKVKGKIVVCLRG 71
            RH +GASLSEKGLP+QKFYPLINSVDAKVQNFSNKYAQLCEIESLDPEKVKGKIVVCLRG
Sbjct: 1094 RHFKGASLSEKGLPVQKFYPLINSVDAKVQNFSNKYAQLCEIESLDPEKVKGKIVVCLRG 1153

Query: 72   DNARTEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYSDGQLVYQYINSTKI 131
            DNARTEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYSDGQLVYQYINSTKI
Sbjct: 1154 DNARTEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYSDGQLVYQYINSTKI 1213

Query: 132  PMAYLTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGENILAAYSEDGSPSGL 191
            PMAYLTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGENILAAYSEDGSPSGL
Sbjct: 1214 PMAYLTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGENILAAYSEDGSPSGL 1273

Query: 192  YFDKRRVPFSVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLNPIL 251
            YFDKRRVPF+VESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLNPIL
Sbjct: 1274 YFDKRRVPFNVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLNPIL 1333

Query: 252  STKQEKANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYKIKQIKQFSNDTSFV 311
            STKQEKANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYKIKQIKQFSNDTSFV
Sbjct: 1334 STKQEKANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYKIKQIKQFSNDTSFV 1393

Query: 312  CSKSFKIIDLNYPSISIPNLESDVALKIKRRLKNVGSPGTYVVQVNAPLGVSVSVEPTSL 371
            CSKSFKI DLNYPSISIPNLESDVALKIKRRLKNVGSPGTYVVQVNAPLGVSVSVEPTSL
Sbjct: 1394 CSKSFKITDLNYPSISIPNLESDVALKIKRRLKNVGSPGTYVVQVNAPLGVSVSVEPTSL 1453

Query: 372  KFTGIDEEKSFRVVLKSNVPNGFHPEYVFGKLEWSDGNNHRVRSPIVVKLGK 423
            KFTGIDEEKSFRVVLKSNVPNGFHPEYVFGKLEWSDGNNHRVRSPIVVKLGK
Sbjct: 1454 KFTGIDEEKSFRVVLKSNVPNGFHPEYVFGKLEWSDGNNHRVRSPIVVKLGK 1505

BLAST of Cmc06g0175491 vs. ExPASy TrEMBL
Match: A0A0A0L2R9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045120 PE=3 SV=1)

HSP 1 Score: 688.0 bits (1774), Expect = 2.6e-194
Identity = 342/411 (83.21%), Postives = 371/411 (90.27%), Query Frame = 0

Query: 12  RH-QGASLSEKGLPIQKFYPLINSVDAKVQNFSNKYAQLCEIESLDPEKVKGKIVVCLRG 71
           RH +GAS+S+K LP+QKFYPLI+S+DAK +N ++  A LCE  SLDP+KV GKI++CLRG
Sbjct: 350 RHFKGASVSDKKLPVQKFYPLISSLDAKAKNVTDNDALLCEEGSLDPKKVNGKIIICLRG 409

Query: 72  DNARTEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYSDGQLVYQYINSTKI 131
           DNAR  KGYV AKAG VGMILANAEENGD+I ADAHLLPASHITYSDGQLVYQYINSTKI
Sbjct: 410 DNARVAKGYVAAKAGAVGMILANAEENGDEILADAHLLPASHITYSDGQLVYQYINSTKI 469

Query: 132 PMAYLTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGENILAAYSEDGSPSGL 191
           PMAY+TH RTE G+KPAP+MASFSSRGPN++DPSILKPDITAPGENILAAYS D SP+G 
Sbjct: 470 PMAYMTHVRTEEGIKPAPVMASFSSRGPNTVDPSILKPDITAPGENILAAYSRDASPTGT 529

Query: 192 YFDKRRVPFSVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLNPIL 251
            FDKRRVPF+VESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDL PIL
Sbjct: 530 DFDKRRVPFNVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLTPIL 589

Query: 252 STKQEKANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYKIKQIKQFSNDTSFV 311
           ST QEKAN +AYGAGHV PN+AADPGLVYDLST+DYLNYLCA GY   QIKQFSNDTSFV
Sbjct: 590 STNQEKANAFAYGAGHVSPNRAADPGLVYDLSTKDYLNYLCAQGYNTAQIKQFSNDTSFV 649

Query: 312 CSKSFKIIDLNYPSISIPNLESDVALKIKRRLKNVGSPGTYVVQVNAPLGVSVSVEPTSL 371
           CSKSFK+ DLNYPSISIP LE DVA+KIKR+LKNVGSPGTYVVQV  PLGVSVSVEPTSL
Sbjct: 650 CSKSFKLTDLNYPSISIPILEYDVAVKIKRKLKNVGSPGTYVVQVKEPLGVSVSVEPTSL 709

Query: 372 KFTGIDEEKSFRVVLKSNVPNGFHPEYVFGKLEWSDGNNHRVRSPIVVKLG 422
           KFTGIDEEKSFRVVLKS  PNG  P+Y+FGKLEWSDG  HRVRSPIVV+LG
Sbjct: 710 KFTGIDEEKSFRVVLKSYEPNGSGPKYLFGKLEWSDG-KHRVRSPIVVRLG 759

BLAST of Cmc06g0175491 vs. ExPASy TrEMBL
Match: A0A6J1CQB4 (subtilisin-like protease SBT5.4 OS=Momordica charantia OX=3673 GN=LOC111013335 PE=3 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 1.2e-162
Identity = 296/413 (71.67%), Postives = 343/413 (83.05%), Query Frame = 0

Query: 10  NYRH-QGASLSEKGLPIQKFYPLINSVDAKVQNFSNKYAQLCEIESLDPEKVKGKIVVCL 69
           N +H +GASLS+K LP QKFYPLI++ DAK  N S +YAQLCE  SLDP+KV+GKIVVCL
Sbjct: 360 NRKHIKGASLSDKILPAQKFYPLISTADAKASNVSLEYAQLCEEGSLDPKKVEGKIVVCL 419

Query: 70  RGDNARTEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYSDGQLVYQYINST 129
           RGDNAR +KGYV A+AG VGMILANA++NGD++ ADAHLLPASH++Y+DG+L++QYI ST
Sbjct: 420 RGDNARVDKGYVAAQAGAVGMILANAQDNGDELLADAHLLPASHVSYADGELIFQYIKST 479

Query: 130 KIPMAYLTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGENILAAYSEDGSPS 189
           KIPMAY+TH +TE GVKPAP MASFSSRGPN+I+ SILKPDI APG +I+AAYSE+ SPS
Sbjct: 480 KIPMAYMTHVKTELGVKPAPFMASFSSRGPNTIEESILKPDIIAPGVSIIAAYSEEASPS 539

Query: 190 GLYFDKRRVPFSVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLNP 249
           G  FDKRR PF+ ESGTSMSCPHVSGIVGLLKT YPKWSPAAI+SA+MTTA TKANDL+P
Sbjct: 540 GSSFDKRRSPFNAESGTSMSCPHVSGIVGLLKTRYPKWSPAAIKSALMTTAATKANDLHP 599

Query: 250 ILSTKQEKANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYKIKQIKQFSNDTS 309
           IL+T Q KAN  +YGAGHVRPNKA +PGLVYDL+ +DYLN+LCA GY   QIK+FS+   
Sbjct: 600 ILNTTQLKANPLSYGAGHVRPNKALNPGLVYDLTLKDYLNFLCARGYNQTQIKKFSS-AP 659

Query: 310 FVCSKSFKIIDLNYPSISIPNLESDVALKIKRRLKNVGSPGTYVVQVNAPLGVSVSVEPT 369
           F CS SFK+ D NYPSISIPNL+    +K KRR+KNVGSPGTYV QV AP GV+VSVEP 
Sbjct: 660 FACSHSFKLTDFNYPSISIPNLKFG-PVKAKRRVKNVGSPGTYVAQVKAPPGVAVSVEPN 719

Query: 370 SLKFTGIDEEKSFRVVLKSNVPNGFHPEYVFGKLEWSDGNNHRVRSPIVVKLG 422
            LKFTGI EE+SFRVV++  V N     YVFG L WSDG NHRVRSPI V LG
Sbjct: 720 MLKFTGIGEEQSFRVVVR-RVENEERRGYVFGWLAWSDG-NHRVRSPIAVNLG 768

BLAST of Cmc06g0175491 vs. ExPASy TrEMBL
Match: A0A0A0L613 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045110 PE=3 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 1.3e-161
Identity = 288/413 (69.73%), Postives = 342/413 (82.81%), Query Frame = 0

Query: 10  NYRH-QGASLSEKGLPIQKFYPLINSVDAKVQNFSNKYAQLCEIESLDPEKVKGKIVVCL 69
           N +H +GASLS+K LP QKFYPLI++VDAK    S+  AQLC + SLDPEKVKGKI++CL
Sbjct: 224 NKKHIKGASLSDKILPEQKFYPLIDAVDAKANKVSSDIAQLCLVGSLDPEKVKGKIIICL 283

Query: 70  RGDNARTEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYSDGQLVYQYINST 129
           RG+NAR +KGY   KAG VGMILANAEENGD+I ADAHLLP SH++Y+DGQ +YQYINST
Sbjct: 284 RGENARADKGYAAVKAGAVGMILANAEENGDEIIADAHLLPVSHVSYTDGQSIYQYINST 343

Query: 130 KIPMAYLTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGENILAAYSEDGSPS 189
           K PMAY+TH RTE G+KPAP+MASFSSRGPN+++ +ILKPDITAPG NILAAYSED SPS
Sbjct: 344 KTPMAYMTHVRTELGIKPAPVMASFSSRGPNTVEETILKPDITAPGVNILAAYSEDASPS 403

Query: 190 GLYFDKRRVPFSVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLNP 249
           G +FDKRR+PF++ SGTSMSCPH+SGIVGLLKTLYP WSPAAI+SAIMTTA ++AND++P
Sbjct: 404 GSFFDKRRIPFNIVSGTSMSCPHISGIVGLLKTLYPNWSPAAIKSAIMTTAESRANDMHP 463

Query: 250 ILSTKQEKANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYKIKQIKQFSNDTS 309
           I +    KAN +AYGAGHV+PN+A +PGLVYDL+T+DY+N+LCA GY   QI +FS   S
Sbjct: 464 IQNGGNLKANSFAYGAGHVQPNRAMNPGLVYDLTTKDYMNFLCAQGYNKSQISKFS-AMS 523

Query: 310 FVCSKSFKIIDLNYPSISIPNLESDVALKIKRRLKNVGSPGTYVVQVNAPLGVSVSVEPT 369
           FVCSKSFK+ D NYPSISIP+++S V + IKRR+KNVG P TYV +V  P GVSVSVEP 
Sbjct: 524 FVCSKSFKLTDFNYPSISIPDMKSGV-VTIKRRVKNVGKPSTYVARVKVPHGVSVSVEPR 583

Query: 370 SLKFTGIDEEKSFRVVLKSNVPNGFHPEYVFGKLEWSDGNNHRVRSPIVVKLG 422
           +LKFT IDEEKSF+VV+ S V N  H  YVFG L W DG +H VRSPIVV LG
Sbjct: 584 TLKFTRIDEEKSFKVVIGS-VANNKHKGYVFGSLIWEDGKHH-VRSPIVVNLG 632

BLAST of Cmc06g0175491 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 441.0 bits (1133), Expect = 1.0e-123
Identity = 230/423 (54.37%), Postives = 296/423 (69.98%), Query Frame = 0

Query: 2   FLLSLMIFNYRH-QGASLSEKGLPIQKFYPLINSVDAKVQNFSNKYAQLCEIESLDPEKV 61
           F  +L++ N +H +G SLS   LP  KFYP++ SV+AK +N S   AQLC++ SLDP K 
Sbjct: 356 FASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKT 415

Query: 62  KGKIVVCLRGDNARTEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYSDGQL 121
           KGKI+VCLRG N R EKG  VA  GG+GM+L N    G+D+ AD H+LPA+ +T  D   
Sbjct: 416 KGKILVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFA 475

Query: 122 VYQYINSTKIPMAYLTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGENILAA 181
           V +YI+ TK P+A++T  RT+ G+KPAP+MASFSS+GP+ + P ILKPDITAPG +++AA
Sbjct: 476 VSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAA 535

Query: 182 YSEDGSPSGLYFDKRRVPFSVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAG 241
           Y+   SP+   FD RR+ F+  SGTSMSCPH+SGI GLLKT YP WSPAAIRSAIMTTA 
Sbjct: 536 YTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTAT 595

Query: 242 TKANDLNPILSTKQEKANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYKIKQI 301
              +   PI +    KA  +++GAGHV+PN A +PGLVYDL  +DYLN+LC+LGY   QI
Sbjct: 596 IMDDIPGPIQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQI 655

Query: 302 KQFSNDTSFVCSKSFKIIDLNYPSISIPNLESDVALKIKRRLKNVGSPGTYVVQVNAPLG 361
             FS +     S    +++LNYPSI++PNL S   + + R +KNVG P  Y V+VN P G
Sbjct: 656 SVFSGNNFTCSSPKISLVNLNYPSITVPNLTSS-KVTVSRTVKNVGRPSMYTVKVNNPQG 715

Query: 362 VSVSVEPTSLKFTGIDEEKSFRVVL---KSNVPNGFHPEYVFGKLEWSDGNNHRVRSPIV 421
           V V+V+PTSL FT + E+K+F+V+L   K NV  G    YVFG+L WSD   HRVRSPIV
Sbjct: 716 VYVAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKG----YVFGELVWSD-KKHRVRSPIV 772

BLAST of Cmc06g0175491 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 438.3 bits (1126), Expect = 6.8e-123
Identity = 223/409 (54.52%), Postives = 289/409 (70.66%), Query Frame = 0

Query: 14  QGASLSEKGLPIQKFYPLINSVDAKVQNFSNKYAQLCEIESLDPEKVKGKIVVCLRGDNA 73
           +G SLS K LP +K Y LI++ DA V N +   A LC+  SLDP+KVKGKI+VCLRGDNA
Sbjct: 376 KGTSLS-KPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNA 435

Query: 74  RTEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYSDGQLVYQYINSTKIPMA 133
           R +KG   A AG  GM+L N + +G++I +DAH+LPAS I Y DG+ ++ Y++STK P  
Sbjct: 436 RVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKG 495

Query: 134 YLTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGENILAAYSEDGSPSGLYFD 193
           Y+  P      KPAP MASFSSRGPN+I P ILKPDITAPG NI+AA++E   P+ L  D
Sbjct: 496 YIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSD 555

Query: 194 KRRVPFSVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLNPILSTK 253
            RR PF+ ESGTSMSCPH+SG+VGLLKTL+P WSPAAIRSAIMTT+ T+ N   P++   
Sbjct: 556 NRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDES 615

Query: 254 QEKANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYKIKQIKQFSNDTSFVCSK 313
            +KAN ++YG+GHV+PNKAA PGLVYDL+T DYL++LCA+GY    ++ F+ D  + C +
Sbjct: 616 FKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQ 675

Query: 314 SFKIIDLNYPSISIPNLESDVALKIKRRLKNVGSPGTYVVQVNAPLGVSVSVEPTSLKFT 373
              ++D NYPSI++PNL   +   + R+LKNVG P TY  +   PLGV VSVEP  L F 
Sbjct: 676 GANLLDFNYPSITVPNLTGSIT--VTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFN 735

Query: 374 GIDEEKSFRVVLK--SNVPNGFHPEYVFGKLEWSDGNNHRVRSPIVVKL 421
              E K F++ L+     P+G    YVFG+L W+D ++H VRSPIVV+L
Sbjct: 736 KTGEVKIFQMTLRPLPVTPSG----YVFGELTWTD-SHHYVRSPIVVQL 776

BLAST of Cmc06g0175491 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 349.7 bits (896), Expect = 3.2e-96
Identity = 199/410 (48.54%), Postives = 266/410 (64.88%), Query Frame = 0

Query: 15  GASLSEKGLPIQKFYPLINSVDAKVQNFSNKYAQLCEIESLDPEKVKGKIVVCLRGDNAR 74
           G SL +      K  P I + +A   N +N    LC   +L PEKVKGKIV+C RG NAR
Sbjct: 355 GVSLFKGEALPDKLLPFIYAGNA--SNATN--GNLCMTGTLIPEKVKGKIVMCDRGINAR 414

Query: 75  TEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYSDGQLVYQYINSTKIPMAY 134
            +KG VV  AGGVGMILAN   NG+++ ADAHLLPA+ +    G ++  Y+ +   P A 
Sbjct: 415 VQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTAS 474

Query: 135 LTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGENILAAYSEDGSPSGLYFDK 194
           ++   T  GVKP+P++A+FSSRGPNSI P+ILKPD+ APG NILAA++    P+GL  D 
Sbjct: 475 ISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDS 534

Query: 195 RRVPFSVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLNPILSTKQ 254
           RRV F++ SGTSMSCPHVSG+  LLK+++P+WSPAAIRSA+MTTA     D  P+L    
Sbjct: 535 RRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIAT 594

Query: 255 EK-ANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYKIKQIKQFSNDTSFVC-- 314
            K +  + +GAGHV P  A +PGL+YDL+TEDYL +LCAL Y   QI+  S   ++ C  
Sbjct: 595 GKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSR-RNYTCDP 654

Query: 315 SKSFKIIDLNYPSISIPNLESDVALKIKRRLKNVGSPGTYVVQVNA-PLGVSVSVEPTSL 374
           SKS+ + DLNYPS ++ N++   A K  R + +VG  GTY V+V +   GV +SVEP  L
Sbjct: 655 SKSYSVADLNYPSFAV-NVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVL 714

Query: 375 KFTGIDEEKSFRV--VLKSNVPNGFHPEYVFGKLEWSDGNNHRVRSPIVV 419
            F   +E+KS+ V   + S+ P+G +    FG +EWSDG  H V SP+ +
Sbjct: 715 NFKEANEKKSYTVTFTVDSSKPSGSNS---FGSIEWSDG-KHVVGSPVAI 754

BLAST of Cmc06g0175491 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 311.2 bits (796), Expect = 1.3e-84
Identity = 169/373 (45.31%), Postives = 228/373 (61.13%), Query Frame = 0

Query: 47  AQLCEIESLDPEKVKGKIVVCLRGDNARTEKGYVVAKAGGVGMILANAEENGDDISADAH 106
           + LC   SLD   V+GKIVVC RG NAR EKG VV  AGG+GMI+AN   +G+++ AD+H
Sbjct: 377 SNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSH 436

Query: 107 LLPASHITYSDGQLVYQYINSTKIPMAYLTHPRTESGVKPAPIMASFSSRGPNSIDPSIL 166
           LLPA  +    G L+ +Y+ S   P A L    T   VKP+P++A+FSSRGPN++ P IL
Sbjct: 437 LLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEIL 496

Query: 167 KPDITAPGENILAAYSEDGSPSGLYFDKRRVPFSVESGTSMSCPHVSGIVGLLKTLYPKW 226
           KPD+  PG NILA +S+   P+GL  D RR  F++ SGTSMSCPH+SG+ GLLK  +P+W
Sbjct: 497 KPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEW 556

Query: 227 SPAAIRSAIMTTAGTKANDLNPILSTKQEK-ANEWAYGAGHVRPNKAADPGLVYDLSTED 286
           SP+AI+SA+MTTA    N   P+        +N +A+G+GHV P KA  PGLVYD+STE+
Sbjct: 557 SPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEE 616

Query: 287 YLNYLCALGYKIKQIKQFSNDTSFVCSKSFKII-DLNYPSISIPNLESDVALKIKRRLKN 346
           Y+ +LC+L Y +  I       S  CSK F     LNYPS S+        ++  R + N
Sbjct: 617 YIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSV-LFGGKRVVRYTREVTN 676

Query: 347 VGSPGT-YVVQVNAPLGVSVSVEPTSLKFTGIDEEKSFRVVLKSNVPNGFHPEYVFGKLE 406
           VG+  + Y V VN    V +SV+P+ L F  + E+K + V   S        +  FG + 
Sbjct: 677 VGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSIT 736

Query: 407 WSDGNNHRVRSPI 417
           WS+   H VRSP+
Sbjct: 737 WSN-PQHEVRSPV 747

BLAST of Cmc06g0175491 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 308.5 bits (789), Expect = 8.1e-84
Identity = 177/418 (42.34%), Postives = 251/418 (60.05%), Query Frame = 0

Query: 11  YRHQGASLSEKGLPIQ-KFYPLINSVDAKVQNFSNKYAQLCEIESLDPEKVKGKIVVCLR 70
           +R +G SL   G+P+  + +P++    + + +     A LC   +LDP++V+GKIV+C R
Sbjct: 348 HRLRGVSL-YAGVPLNGRMFPVVYPGKSGMSS-----ASLCMENTLDPKQVRGKIVICDR 407

Query: 71  GDNARTEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYSDGQLVYQYINSTK 130
           G + R  KG VV KAGGVGMILAN   NG+ +  DAHL+PA  +  ++G  +  Y +S  
Sbjct: 408 GSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHP 467

Query: 131 IPMAYLTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGENILAAYSEDGSPSG 190
            P+A +    T  G+KPAP++ASFS RGPN + P ILKPD+ APG NILAA+++   P+G
Sbjct: 468 NPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTG 527

Query: 191 LYFDKRRVPFSVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLNPI 250
           L  D R+  F++ SGTSM+CPHVSG   LLK+ +P WSPA IRSA+MTT     N    +
Sbjct: 528 LPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSL 587

Query: 251 LSTKQEK-ANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYKIKQIKQFSNDTS 310
           +     K A  + YG+GH+   +A +PGLVYD++ +DY+ +LC++GY  K I Q    T 
Sbjct: 588 IDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTI-QVITRTP 647

Query: 311 FVCSKSFKII--DLNYPSISI---PNLESDVALKIKRRLKNVG-SPGTYVVQVNAPLGVS 370
             C  + K    +LNYPSI+     N    V+  + R   NVG +   Y  ++ +P GV+
Sbjct: 648 VRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVT 707

Query: 371 VSVEPTSLKFTGIDEEKSFRVVLKSNVPNGFHPE--YVFGKLEWSDGNNHRVRSPIVV 419
           V+V+P  L FT   + +S+ V +  N  N    E   VFG + W DG  H VRSPIVV
Sbjct: 708 VTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVV 758

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TYK19382.16.8e-23499.51subtilisin-like protease SBT5.3 [Cucumis melo var. makuwa][more]
XP_016900594.12.0e-23398.79PREDICTED: subtilisin-like protease SBT5.3, partial [Cucumis melo][more]
KAE8650095.15.3e-19483.21hypothetical protein Csa_011146 [Cucumis sativus][more]
XP_011650462.25.3e-19483.21subtilisin-like protease SBT5.4 [Cucumis sativus][more]
XP_038904154.11.7e-19283.58subtilisin-like protease SBT5.4 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9ZSP51.5e-12254.37Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=... [more]
F4JXC59.6e-12254.52Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S... [more]
O653514.5e-9548.54Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... [more]
I1N4621.6e-9247.80Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P... [more]
Q9ZUF61.8e-8345.31Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A5D3D7733.3e-23499.51Subtilisin-like protease SBT5.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S4DX859.6e-23498.79subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491136 PE=3 SV=... [more]
A0A0A0L2R92.6e-19483.21Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045120 PE=3 SV=1[more]
A0A6J1CQB41.2e-16271.67subtilisin-like protease SBT5.4 OS=Momordica charantia OX=3673 GN=LOC111013335 P... [more]
A0A0A0L6131.3e-16169.73Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045110 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G04160.11.0e-12354.37Subtilisin-like serine endopeptidase family protein [more]
AT5G59810.16.8e-12354.52Subtilase family protein [more]
AT5G67360.13.2e-9648.54Subtilase family protein [more]
AT2G05920.11.3e-8445.31Subtilase family protein [more]
AT4G34980.18.1e-8442.34subtilisin-like serine protease 2 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003137PA domainPFAMPF02225PAcoord: 50..119
e-value: 7.1E-12
score: 45.2
NoneNo IPR availableGENE3D2.60.40.2310coord: 290..420
e-value: 5.7E-35
score: 121.9
NoneNo IPR availableGENE3D3.50.30.30coord: 14..142
e-value: 8.0E-85
score: 287.3
NoneNo IPR availablePANTHERPTHR10795:SF662SUBTILISIN-LIKE PROTEASE SBT5.4coord: 10..421
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 1..273
score: 10.955145
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 15..135
e-value: 1.07067E-37
score: 131.382
NoneNo IPR availableSUPERFAMILY52025PA domaincoord: 53..131
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 319..418
e-value: 8.0E-26
score: 90.1
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 143..286
e-value: 8.0E-85
score: 287.3
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 151..329
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 38..251
e-value: 2.8E-22
score: 79.8
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 10..421
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 204..214

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc06g0175491.1Cmc06g0175491.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0009610 response to symbiotic fungus
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity