Cmc04g0117531 (gene) Melon (Charmono) v1.1

Overview
NameCmc04g0117531
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionRibonuclease T(2)
LocationCMiso1.1chr04: 34650350 .. 34651422 (-)
RNA-Seq ExpressionCmc04g0117531
SyntenyCmc04g0117531
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCATCTTCTATTTTATTGTTTTCTTCTCTCTTCGATAATGGCTCAACGTATTTTAGTTATTTACTTTTTAAGTTTGGTTTTGGTTGTTAATGCCGGTGTTCCGTTCGACTTTTTCCAATTCGTGCAACAGTGGGGGCTGAATGTATGTAATACTGGCGCTCATGTCTCCCCTTGTCATACTCCCCCTCGACCCATGTTCACTATTCACGGCTTGTGGCCAAGTAATCATAGTAATGCTCATCTTGTTTGTTGGTCAAAAACGCAATATAGCAAGGTTCCCATCTTCTTTAATATTTGTGTATGTGTTTTTTTTTCCCTCTATCTTCAACATTTTAACTAATATAATGTTGTAACATGCTTTAATTTTAGGTTATCTCACTTGAAGCACAACTGAAAATATATTGGCCAGATTTAATATCTGGGAACAATGATAAATTTTGGGGTCATGAATGGGACAATCATGGGAAATGTTCAGATCCTCCTTTTGGCTTATTTCAATATTTCCAAATTTCTTTGAATCTTCGGAAACAATTTGATCTGTTGGCAATCTTAACCGCCGCGGGGCTCAACCCTCAAAGCTCCAATGACCTTGTAATCCAAGACATTATAACCCCTATTCAAAAGATAACAAAGACGGAACCCGGCATCCGCTGCAACAAAAATGGTAGAACTGGCAAGTTGCAATTGTATGAAATTGTACTGTGTTTGGAAAAGGATGGCGTTACCCTCATCGACTGCCCTTCATTTGTTAGTGGCACTTGTCCAACTAAATTTGTGTGGCTCCTACCACAAGAGAACTCGTTTGGTGCTTCAGATTATCCATGAGTCATATGGGAAAAGTTATGTTATATTGCTTATTATTATGCTTAGCAGATGACTATATTTGCAGAACTTTAAGTTTCTTCTTTATATTAAATCGGTATGTTATGCTTTCACTTTGAATCATTTGCTTTTGGACGTCCAAACAAGCTAAAGCCTCTCCTCCGTGTTTGTAATATTAAATTTTAGTATAATTGTAATATTAAGTAACCTTTAATTAGTTTTGACTATATATATTAATTTTTGAAACAGAA

mRNA sequence

TCATCTTCTATTTTATTGTTTTCTTCTCTCTTCGATAATGGCTCAACGTATTTTAGTTATTTACTTTTTAAGTTTGGTTTTGGTTGTTAATGCCGGTGTTCCGTTCGACTTTTTCCAATTCGTGCAACAGTGGGGGCTGAATGTATGTAATACTGGCGCTCATGTCTCCCCTTGTCATACTCCCCCTCGACCCATGTTCACTATTCACGGCTTGTGGCCAAGTAATCATAGTAATGCTCATCTTGTTTGTTGGTCAAAAACGCAATATAGCAAGGTTATCTCACTTGAAGCACAACTGAAAATATATTGGCCAGATTTAATATCTGGGAACAATGATAAATTTTGGGGTCATGAATGGGACAATCATGGGAAATGTTCAGATCCTCCTTTTGGCTTATTTCAATATTTCCAAATTTCTTTGAATCTTCGGAAACAATTTGATCTGTTGGCAATCTTAACCGCCGCGGGGCTCAACCCTCAAAGCTCCAATGACCTTGTAATCCAAGACATTATAACCCCTATTCAAAAGATAACAAAGACGGAACCCGGCATCCGCTGCAACAAAAATGGTAGAACTGGCAAGTTGCAATTGTATGAAATTGTACTGTGTTTGGAAAAGGATGGCGTTACCCTCATCGACTGCCCTTCATTTGTTAGTGGCACTTGTCCAACTAAATTTGTGTGGCTCCTACCACAAGAGAACTCGTTTGGTGCTTCAGATTATCCATGAGTCATATGGGAAAAGTTATGTTATATTGCTTATTATTATGCTTAGCAGATGACTATATTTGCAGAACTTTAAGTTTCTTCTTTATATTAAATCGGTATGTTATGCTTTCACTTTGAATCATTTGCTTTTGGACGTCCAAACAAGCTAAAGCCTCTCCTCCGTGTTTGTAATATTAAATTTTAGTATAATTGTAATATTAAGTAACCTTTAATTAGTTTTGACTATATATATTAATTTTTGAAACAGAA

Coding sequence (CDS)

ATGGCTCAACGTATTTTAGTTATTTACTTTTTAAGTTTGGTTTTGGTTGTTAATGCCGGTGTTCCGTTCGACTTTTTCCAATTCGTGCAACAGTGGGGGCTGAATGTATGTAATACTGGCGCTCATGTCTCCCCTTGTCATACTCCCCCTCGACCCATGTTCACTATTCACGGCTTGTGGCCAAGTAATCATAGTAATGCTCATCTTGTTTGTTGGTCAAAAACGCAATATAGCAAGGTTATCTCACTTGAAGCACAACTGAAAATATATTGGCCAGATTTAATATCTGGGAACAATGATAAATTTTGGGGTCATGAATGGGACAATCATGGGAAATGTTCAGATCCTCCTTTTGGCTTATTTCAATATTTCCAAATTTCTTTGAATCTTCGGAAACAATTTGATCTGTTGGCAATCTTAACCGCCGCGGGGCTCAACCCTCAAAGCTCCAATGACCTTGTAATCCAAGACATTATAACCCCTATTCAAAAGATAACAAAGACGGAACCCGGCATCCGCTGCAACAAAAATGGTAGAACTGGCAAGTTGCAATTGTATGAAATTGTACTGTGTTTGGAAAAGGATGGCGTTACCCTCATCGACTGCCCTTCATTTGTTAGTGGCACTTGTCCAACTAAATTTGTGTGGCTCCTACCACAAGAGAACTCGTTTGGTGCTTCAGATTATCCATGA

Protein sequence

MAQRILVIYFLSLVLVVNAGVPFDFFQFVQQWGLNVCNTGAHVSPCHTPPRPMFTIHGLWPSNHSNAHLVCWSKTQYSKVISLEAQLKIYWPDLISGNNDKFWGHEWDNHGKCSDPPFGLFQYFQISLNLRKQFDLLAILTAAGLNPQSSNDLVIQDIITPIQKITKTEPGIRCNKNGRTGKLQLYEIVLCLEKDGVTLIDCPSFVSGTCPTKFVWLLPQENSFGASDYP
Homology
BLAST of Cmc04g0117531 vs. NCBI nr
Match: KAE8651058.1 (hypothetical protein Csa_002465 [Cucumis sativus])

HSP 1 Score: 362.8 bits (930), Expect = 2.1e-96
Identity = 169/229 (73.80%), Postives = 185/229 (80.79%), Query Frame = 0

Query: 1   MAQRILVIYFLSLVLVVNAGVPFDFFQFVQQWGLNVCNTGAHVSPCHTPPRPMFTIHGLW 60
           M Q IL+IYFLSLV+ VN GVPF+FFQFV+QWG NVCNTGA VSPCHTPP+  FTIHGLW
Sbjct: 1   MTQSILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLW 60

Query: 61  PSNHSNAHLVCWSKTQYSKVISLEAQLKIYWPDLISGNNDKFWGHEWDNHGKCSDPPFGL 120
           PSNHSNAHL+C SKT YSKVISLE QL I WPDL  GNNDKFWGHEWD HGKCSDPPF L
Sbjct: 61  PSNHSNAHLLCQSKTIYSKVISLEGQLNISWPDLKYGNNDKFWGHEWDTHGKCSDPPFSL 120

Query: 121 FQYFQISLNLRKQFDLLAILTAAGLNPQSSNDLVIQDIITPIQKITKTEPGIRCNKNGRT 180
           FQYFQISLNL ++FDLL IL AAGLNPQ+S +L IQ+I+ PIQ+ TK  PGIRCNKN +T
Sbjct: 121 FQYFQISLNLLRKFDLLTILKAAGLNPQTSQNLAIQNIMAPIQRTTKKYPGIRCNKNVKT 180

Query: 181 GKLQLYEIVLCLEKDGVTLIDCPSFVSGTCPTKFVWLLPQENSFGASDY 230
           GK QL EIVLCLEKDG TLIDCP+FVS TC   FVW       FG   +
Sbjct: 181 GKSQLNEIVLCLEKDGATLIDCPTFVSNTCAKSFVWRDVGGQEFGGISF 229

BLAST of Cmc04g0117531 vs. NCBI nr
Match: XP_031738690.1 (ribonuclease MC-like [Cucumis sativus])

HSP 1 Score: 361.3 bits (926), Expect = 6.2e-96
Identity = 167/216 (77.31%), Postives = 182/216 (84.26%), Query Frame = 0

Query: 1   MAQRILVIYFLSLVLVVNAGVPFDFFQFVQQWGLNVCNTGAHVSPCHTPPRPMFTIHGLW 60
           M Q IL+IYFLSLV+ VN GVPF+FFQFV+QWG NVCNTGA VSPCHTPP+  FTIHGLW
Sbjct: 1   MTQSILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLW 60

Query: 61  PSNHSNAHLVCWSKTQYSKVISLEAQLKIYWPDLISGNNDKFWGHEWDNHGKCSDPPFGL 120
           PSNHSNAHL+C SKT YSKVISLE QL I WPDL  GNNDKFWGHEWD HGKCSDPPF L
Sbjct: 61  PSNHSNAHLLCQSKTIYSKVISLEGQLNISWPDLKYGNNDKFWGHEWDTHGKCSDPPFSL 120

Query: 121 FQYFQISLNLRKQFDLLAILTAAGLNPQSSNDLVIQDIITPIQKITKTEPGIRCNKNGRT 180
           FQYFQISLNL ++FDLL IL AAGLNPQ+S +L IQ+I+ PIQ+ TK  PGIRCNKN +T
Sbjct: 121 FQYFQISLNLLRKFDLLTILKAAGLNPQTSQNLAIQNIMAPIQRTTKKYPGIRCNKNVKT 180

Query: 181 GKLQLYEIVLCLEKDGVTLIDCPSFVSGTCPTKFVW 217
           GK QL EIVLCLEKDG TLIDCP+FVS TC   FVW
Sbjct: 181 GKSQLNEIVLCLEKDGATLIDCPTFVSNTCAKSFVW 216

BLAST of Cmc04g0117531 vs. NCBI nr
Match: XP_031738547.1 (ribonuclease MC-like [Cucumis sativus] >XP_031739193.1 ribonuclease MC-like [Cucumis sativus] >KAE8651059.1 hypothetical protein Csa_001394 [Cucumis sativus] >KAE8651065.1 hypothetical protein Csa_001488 [Cucumis sativus])

HSP 1 Score: 336.7 bits (862), Expect = 1.6e-88
Identity = 155/216 (71.76%), Postives = 179/216 (82.87%), Query Frame = 0

Query: 1   MAQRILVIYFLSLVLVVNAGVPFDFFQFVQQWGLNVCNTGAHVSPCHTPPRPMFTIHGLW 60
           M Q IL+IYFLSLV+ VN GVPF+FFQFV+QWG NVCNTGA VSPCHTPP+  FTIHGLW
Sbjct: 1   MTQSILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLW 60

Query: 61  PSNHSNAHLVCWSKTQYSKVISLEAQLKIYWPDLISGNNDKFWGHEWDNHGKCSDPPFGL 120
           PSNHSNAHL+C S T+Y+KV+ LE QL I WPDL  G N++FW HEW+ HGKCSDPPF L
Sbjct: 61  PSNHSNAHLLCQSITKYTKVLLLERQLNISWPDLKGGMNNEFWTHEWNFHGKCSDPPFSL 120

Query: 121 FQYFQISLNLRKQFDLLAILTAAGLNPQSSNDLVIQDIITPIQKITKTEPGIRCNKNGRT 180
           FQYFQ+SLNL ++FD+LAIL AAGLNPQ+S ++ IQ+I+TPIQ+ TK  PGIRC KN +T
Sbjct: 121 FQYFQMSLNLLQKFDVLAILKAAGLNPQTSQNIGIQNIMTPIQRTTKKYPGIRCYKNVKT 180

Query: 181 GKLQLYEIVLCLEKDGVTLIDCPSFVSGTCPTKFVW 217
           GK QL EIVLCLEKDG TLIDCP+FVS TC    VW
Sbjct: 181 GKSQLNEIVLCLEKDGATLIDCPTFVSSTCAKSIVW 216

BLAST of Cmc04g0117531 vs. NCBI nr
Match: XP_011651927.1 (ribonuclease MC [Cucumis sativus] >KGN58920.1 hypothetical protein Csa_001884 [Cucumis sativus])

HSP 1 Score: 266.5 bits (680), Expect = 2.1e-67
Identity = 133/237 (56.12%), Postives = 158/237 (66.67%), Query Frame = 0

Query: 1   MAQRILVIYFLSLVLVVNAGVPFDFFQFVQQWGLNVCNTGAHVSPCHTPPRPMFTIHGLW 60
           MAQ I ++  LS +L VN G+PFDFFQ VQQWG N+C+     S C+  P+PMFTIHGLW
Sbjct: 1   MAQHIFILCLLSSLLFVN-GIPFDFFQLVQQWGPNICSPAG--SKCYAQPQPMFTIHGLW 60

Query: 61  PSNHSNAHLVCWSKTQ---YSKVISLEAQLKIYWPDLISGNNDKFWGHEWDNHGKCSDPP 120
           PSN S   ++C    +     KV SLE  L  YWPD+ISG N  FW HEW  H  C DPP
Sbjct: 61  PSNFSTVPILCPRTKKLFPQGKVTSLEQHLNKYWPDVISGKNSNFWEHEWRKHADCIDPP 120

Query: 121 FGLFQYFQISL--NLRKQFDLLAILTAAGLNPQSSNDLVIQDIITPIQKITKTEPGIRCN 180
           F +F YF ISL   + K +DLL IL  AGL+ Q+  DL+ Q+++ PI+  T  EPGIRCN
Sbjct: 121 FTIFWYFDISLRHRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRCN 180

Query: 181 KNGRTGKLQLYEIVLCLEKDGVTLIDCPSFVSGTCPT--KFVWLLPQENSFGASDYP 231
            NG T KLQL EIVLC   DG TLIDCPSFVS TC +  KFVWLLPQ++S G  DYP
Sbjct: 181 INGITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKFVWLLPQQSSVGVPDYP 234

BLAST of Cmc04g0117531 vs. NCBI nr
Match: TYK25090.1 (S-RNase [Cucumis melo var. makuwa])

HSP 1 Score: 249.6 bits (636), Expect = 2.6e-62
Identity = 124/234 (52.99%), Postives = 153/234 (65.38%), Query Frame = 0

Query: 1   MAQRILVIYFLSLVLVVNAGVPFDFFQFVQQWGLNVCNTGAHVSPCHTPPRPMFTIHGLW 60
           MAQ   ++  LSL+L VN G PFDFFQ V+QWG N CN G   + CH  P+P FTIHGLW
Sbjct: 1   MAQHTFILCLLSLLLFVN-GYPFDFFQLVEQWGPNTCNDG---TQCHAQPQPEFTIHGLW 60

Query: 61  PSNHSNAHLVCWSKTQY--SKVISLEAQLKIYWPDLISGNNDKFWGHEWDNHGKCSDPPF 120
           PSN SNA L C ++  +   ++  LE  L  YWPD+I+GNN  FW +EW  HG C DPPF
Sbjct: 61  PSNFSNARLFCATRKLFDNGQISLLEGDLDRYWPDVINGNNRGFWSNEWKKHGACIDPPF 120

Query: 121 GLFQYFQISLNLRKQFDLLAILTAAGLNPQS--SNDLVIQDIITPIQKITKTEPGIRCNK 180
            + QYFQ+SL+  + +DLL IL       +   +N L  +DI+ PI   T    GIRCNK
Sbjct: 121 NITQYFQLSLDHHRTYDLLKILNGVWHRNRQMPNNGLAPKDILNPIFNATNKTAGIRCNK 180

Query: 181 NGRTGKLQLYEIVLCLEKDGVTLIDCPSFVSGTC-PTKFVWLLPQENSFGASDY 230
           NG T KLQL+EIVLC + +G TLIDCP FVS +C   KFVWLLPQ++S G  DY
Sbjct: 181 NGITKKLQLHEIVLCFDNNGTTLIDCPKFVSNSCNQPKFVWLLPQQSSDGVPDY 230

BLAST of Cmc04g0117531 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 4.7e-30
Identity = 74/211 (35.07%), Postives = 113/211 (53.55%), Query Frame = 0

Query: 6   LVIYFLSLVLVVNAGVPFDFFQFVQQWGLNVCNTGAHVS-PCHTPPRPMFTIHGLWPSNH 65
           L++   S+    ++   FDFF FVQQW  + C+T      P    P   F IHGLWP+  
Sbjct: 13  LLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGLWPNYK 72

Query: 66  SNAH-LVCWSKTQY--SKVISLEAQLKIYWPDLI--SGNNDKFWGHEWDNHGKCSDPPFG 125
              +   C +   +  S +  L   +K  WP L   SG+ + FW HEW+ HG CS+    
Sbjct: 73  DGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVID 132

Query: 126 LFQYFQISLNLRKQFDLLAILTAAGLNPQSSNDLVIQDIITPIQKITKTEPGIRCNKNGR 185
             +YFQ +LNL+++ +LL  LT AG+NP   +   ++ I   I++     P + CN++G 
Sbjct: 133 QHEYFQTALNLKQKTNLLGALTKAGINPDGKS-YSLESIRDSIKESIGFTPWVECNRDG- 192

Query: 186 TGKLQLYEIVLCLEKDGVTLIDCPSFVSGTC 211
           +G  QLY++ LC+++ G  LI+CP F  G C
Sbjct: 193 SGNSQLYQVYLCVDRSGSGLIECPVFPHGKC 221

BLAST of Cmc04g0117531 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 129.8 bits (325), Expect = 4.0e-29
Identity = 77/216 (35.65%), Postives = 118/216 (54.63%), Query Frame = 0

Query: 5   ILVIYFLSLVLVVNAGVPFDFFQFVQQWGLNVCNTGAHVS-PCHTPPRPMFTIHGLWPSN 64
           ++++     + V+NA   FDFF FVQQW  + C+T      P    P   F IHGLWP+N
Sbjct: 11  LILLIITQCLSVLNAAKDFDFFYFVQQWPGSYCDTKQSCCYPTTGKPAADFGIHGLWPNN 70

Query: 65  HSNAH-LVCWSKTQY--SKVISLEAQLKIYWPDLI--SGNNDKFWGHEWDNHGKCSDPPF 124
           +   +   C   + Y  S++  L + ++  WP L   SG+   FW HEW+ HG C++   
Sbjct: 71  NDGTYPSNCDPNSPYDQSQISDLISSMQQNWPTLACPSGSGSTFWSHEWEKHGTCAESVL 130

Query: 125 -GLFQYFQISLNLRKQFDLLAILTAAGLNPQ-SSNDLVIQDIITPIQKITKTEPGIRCNK 184
                YF+ +L+L+ Q DLL+IL  A ++P   S DLV  +I   I+      P I+CN 
Sbjct: 131 TNQHAYFKKALDLKNQIDLLSILQGADIHPDGESYDLV--NIRNAIKSAIGYTPWIQCNV 190

Query: 185 NGRTGKLQLYEIVLCLEKDGVTLIDCPSFVSGTCPT 213
           + ++G  QLY++ +C++  G +LI+CP F  G C T
Sbjct: 191 D-QSGNSQLYQVYICVDGSGSSLIECPIFPGGKCGT 223

BLAST of Cmc04g0117531 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 2.0e-28
Identity = 70/198 (35.35%), Postives = 103/198 (52.02%), Query Frame = 0

Query: 23  FDFFQFVQQWGLNVCNTGAHVSPCHTPPRPMFTIHGLWPSNHSNAHLVC--WSKTQYSKV 82
           FD F FVQQW   VC+     S C       FTIHGLWP     +   C   S    +K+
Sbjct: 1   FDSFWFVQQWPPAVCSFQKSGS-CPGSGLRTFTIHGLWPQGSGTSLTNCPQGSPFDITKI 60

Query: 83  ISLEAQLKIYWPDLISGNNDKFWGHEWDNHGKCSDPPFGLFQYFQISLNLRKQFDLLAIL 142
             L++QL   WP+++  NN +FW HEW  HG CS+  F    YF++++++R  +D++  L
Sbjct: 61  SHLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGAL 120

Query: 143 T--AAGLNPQSSNDLVIQDIITPIQKITKTEPGIRCNKNGRTGKLQLYEIVLCLEKDGVT 202
              AAG N ++ +   I+  +    K  K  PG+RC  + +T    L ++V C  +DG T
Sbjct: 121 RPHAAGPNGRTKSRQAIKGFLK--AKFGKF-PGLRCRTDPQTKVSYLVQVVACFAQDGST 180

Query: 203 LIDCPSFVSGTCPTKFVW 217
           LIDC      TC   F++
Sbjct: 181 LIDC---TRDTCGANFIF 191

BLAST of Cmc04g0117531 vs. ExPASy Swiss-Prot
Match: O80323 (Ribonuclease S-3 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.7e-24
Identity = 70/209 (33.49%), Postives = 109/209 (52.15%), Query Frame = 0

Query: 1   MAQRILVIYFLSLVLVVNAGVPFDFFQFVQQWGLNVCNTGAHVSPCHTPPRPMFTIHGLW 60
           M   +++++ L ++++ ++ V +D+FQF QQ+ L VCN+   +  C  PP  +FT+HGLW
Sbjct: 1   MVHVVMMVFLLIVLILCSSTVGYDYFQFTQQYQLAVCNSNRTL--CKDPPDKLFTVHGLW 60

Query: 61  PSNHSNAH-LVCWSKTQYSKVISLEAQLKIYWPDLIS-GNNDKFWGHEWDNHGKCSDPPF 120
           PSN        C  K    +   LE QL+I WP++     N+ FW  EW  HG C  P  
Sbjct: 61  PSNMVGPDPSKCPIKNIRKREKLLEHQLEIIWPNVFDRTKNNLFWDKEWMKHGSCGYPTI 120

Query: 121 -GLFQYFQ--ISLNLRKQFDLLAILTAAGLNPQSSNDLVIQDIITPIQK-ITKTEPGIRC 180
                YF+  I + + K+ ++  IL+ A + P      ++ DI   I+      +P ++C
Sbjct: 121 DNENHYFETVIKMYISKKQNVSRILSKAKIEPDGKKRALL-DIENAIRNGADNKKPKLKC 180

Query: 181 NKNGRTGKLQLYEIVLCLEKDGVTLIDCP 204
            K G T   +L EI LC +K G   IDCP
Sbjct: 181 QKKGTT--TELVEITLCSDKSGEHFIDCP 204

BLAST of Cmc04g0117531 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 2.9e-24
Identity = 71/218 (32.57%), Postives = 106/218 (48.62%), Query Frame = 0

Query: 6   LVIYFLSLVLVVNAGVPFDFFQFVQQWGLNVCNT-GAHVSPCHTPPRPMFTIHGLWPSNH 65
           + I  L  + V +    FDFF FV QW    C++  +   P    P   F IHGLWP+  
Sbjct: 5   IFILALQQLYVQSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPNYK 64

Query: 66  SNAHLVCW-------SKTQYSKVISLEAQLKIYWPDLISGNND--KFWGHEWDNHGKCSD 125
           +      W       S+    +V  L + L+  WP L   +ND  KFW HEW+ HG C++
Sbjct: 65  TGG----WPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAE 124

Query: 126 PPFGLFQYFQISLNLRKQFDLLAILTAAGLNPQSSNDLVIQDIITPIQKITKTEPGIRCN 185
                  YF+  L L+++ +LL  LT AG+ P       ++DI   I+++    PGI CN
Sbjct: 125 SELDQHDYFEAGLKLKQKANLLHALTNAGIKP-DDKFYEMKDIENTIKQVVGFAPGIECN 184

Query: 186 KNGRTGKLQLYEIVLCLEKDGVTLIDCPSFVSGTCPTK 214
           K+  +   QLY+I LC++      I+CP    G C ++
Sbjct: 185 KDS-SHNSQLYQIYLCVDTSASKFINCPVMPHGRCDSR 216

BLAST of Cmc04g0117531 vs. ExPASy TrEMBL
Match: A0A0A0LAN6 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G745530 PE=3 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 3.0e-96
Identity = 167/216 (77.31%), Postives = 182/216 (84.26%), Query Frame = 0

Query: 1   MAQRILVIYFLSLVLVVNAGVPFDFFQFVQQWGLNVCNTGAHVSPCHTPPRPMFTIHGLW 60
           M Q IL+IYFLSLV+ VN GVPF+FFQFV+QWG NVCNTGA VSPCHTPP+  FTIHGLW
Sbjct: 1   MTQSILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLW 60

Query: 61  PSNHSNAHLVCWSKTQYSKVISLEAQLKIYWPDLISGNNDKFWGHEWDNHGKCSDPPFGL 120
           PSNHSNAHL+C SKT YSKVISLE QL I WPDL  GNNDKFWGHEWD HGKCSDPPF L
Sbjct: 61  PSNHSNAHLLCQSKTIYSKVISLEGQLNISWPDLKYGNNDKFWGHEWDTHGKCSDPPFSL 120

Query: 121 FQYFQISLNLRKQFDLLAILTAAGLNPQSSNDLVIQDIITPIQKITKTEPGIRCNKNGRT 180
           FQYFQISLNL ++FDLL IL AAGLNPQ+S +L IQ+I+ PIQ+ TK  PGIRCNKN +T
Sbjct: 121 FQYFQISLNLLRKFDLLTILKAAGLNPQTSQNLAIQNIMAPIQRTTKKYPGIRCNKNVKT 180

Query: 181 GKLQLYEIVLCLEKDGVTLIDCPSFVSGTCPTKFVW 217
           GK QL EIVLCLEKDG TLIDCP+FVS TC   FVW
Sbjct: 181 GKSQLNEIVLCLEKDGATLIDCPTFVSNTCAKSFVW 216

BLAST of Cmc04g0117531 vs. ExPASy TrEMBL
Match: A0A0A0LAB0 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G736680 PE=3 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 1.0e-67
Identity = 133/237 (56.12%), Postives = 158/237 (66.67%), Query Frame = 0

Query: 1   MAQRILVIYFLSLVLVVNAGVPFDFFQFVQQWGLNVCNTGAHVSPCHTPPRPMFTIHGLW 60
           MAQ I ++  LS +L VN G+PFDFFQ VQQWG N+C+     S C+  P+PMFTIHGLW
Sbjct: 1   MAQHIFILCLLSSLLFVN-GIPFDFFQLVQQWGPNICSPAG--SKCYAQPQPMFTIHGLW 60

Query: 61  PSNHSNAHLVCWSKTQ---YSKVISLEAQLKIYWPDLISGNNDKFWGHEWDNHGKCSDPP 120
           PSN S   ++C    +     KV SLE  L  YWPD+ISG N  FW HEW  H  C DPP
Sbjct: 61  PSNFSTVPILCPRTKKLFPQGKVTSLEQHLNKYWPDVISGKNSNFWEHEWRKHADCIDPP 120

Query: 121 FGLFQYFQISL--NLRKQFDLLAILTAAGLNPQSSNDLVIQDIITPIQKITKTEPGIRCN 180
           F +F YF ISL   + K +DLL IL  AGL+ Q+  DL+ Q+++ PI+  T  EPGIRCN
Sbjct: 121 FTIFWYFDISLRHRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRCN 180

Query: 181 KNGRTGKLQLYEIVLCLEKDGVTLIDCPSFVSGTCPT--KFVWLLPQENSFGASDYP 231
            NG T KLQL EIVLC   DG TLIDCPSFVS TC +  KFVWLLPQ++S G  DYP
Sbjct: 181 INGITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKFVWLLPQQSSVGVPDYP 234

BLAST of Cmc04g0117531 vs. ExPASy TrEMBL
Match: A0A5D3DP91 (Ribonuclease T(2) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002140 PE=3 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 1.3e-62
Identity = 124/234 (52.99%), Postives = 153/234 (65.38%), Query Frame = 0

Query: 1   MAQRILVIYFLSLVLVVNAGVPFDFFQFVQQWGLNVCNTGAHVSPCHTPPRPMFTIHGLW 60
           MAQ   ++  LSL+L VN G PFDFFQ V+QWG N CN G   + CH  P+P FTIHGLW
Sbjct: 1   MAQHTFILCLLSLLLFVN-GYPFDFFQLVEQWGPNTCNDG---TQCHAQPQPEFTIHGLW 60

Query: 61  PSNHSNAHLVCWSKTQY--SKVISLEAQLKIYWPDLISGNNDKFWGHEWDNHGKCSDPPF 120
           PSN SNA L C ++  +   ++  LE  L  YWPD+I+GNN  FW +EW  HG C DPPF
Sbjct: 61  PSNFSNARLFCATRKLFDNGQISLLEGDLDRYWPDVINGNNRGFWSNEWKKHGACIDPPF 120

Query: 121 GLFQYFQISLNLRKQFDLLAILTAAGLNPQS--SNDLVIQDIITPIQKITKTEPGIRCNK 180
            + QYFQ+SL+  + +DLL IL       +   +N L  +DI+ PI   T    GIRCNK
Sbjct: 121 NITQYFQLSLDHHRTYDLLKILNGVWHRNRQMPNNGLAPKDILNPIFNATNKTAGIRCNK 180

Query: 181 NGRTGKLQLYEIVLCLEKDGVTLIDCPSFVSGTC-PTKFVWLLPQENSFGASDY 230
           NG T KLQL+EIVLC + +G TLIDCP FVS +C   KFVWLLPQ++S G  DY
Sbjct: 181 NGITKKLQLHEIVLCFDNNGTTLIDCPKFVSNSCNQPKFVWLLPQQSSDGVPDY 230

BLAST of Cmc04g0117531 vs. ExPASy TrEMBL
Match: A0A6J1EXD5 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111439251 PE=3 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 1.3e-54
Identity = 111/221 (50.23%), Postives = 142/221 (64.25%), Query Frame = 0

Query: 1   MAQRILVIYFLSLVLVVN-AGVPFDFFQFVQQWGLNVCNTGAHVSPCHTPPRPMFTIHGL 60
           MAQ   ++     VL+ +     FD+FQ VQQW    C +G     C+  P  MFTIHGL
Sbjct: 1   MAQAPFIVSIFKFVLLTSCVKAQFDYFQMVQQWPPATC-SGVR---CYANPPAMFTIHGL 60

Query: 61  WPSNHSNAHLVCWSKT-QYSKVISLEAQLKIYWPDLISGNNDKFWGHEWDNHGKCSDPPF 120
           WPSN+SN  LVC  +    +++ +L  QL  YWPD+ISGNN +FW HEWD+HG CSDPPF
Sbjct: 61  WPSNYSNVPLVCAGQPFNPAQIATLVPQLNTYWPDVISGNNQRFWKHEWDSHGTCSDPPF 120

Query: 121 GLFQYFQISLNLR--KQFDLLAILTAAGLNPQSSNDLVIQDIITPIQKITKTEPGIRCNK 180
              QYFQ +LN+R    +DLLAIL  AGL P ++N      I   IQ  T  +PG+RCNK
Sbjct: 121 NQLQYFQTTLNIRTNHNYDLLAILNTAGLGPTATNTRQYGAIEGAIQAATGKKPGLRCNK 180

Query: 181 NGRTGKLQLYEIVLCLEKDGVTLIDCPSFVSGTCPTKFVWL 218
           N ++ K QL+EI+LC +K+GVTLIDC  F   TCP++FVWL
Sbjct: 181 NAQSKKKQLFEIILCFDKNGVTLIDCTQFAGITCPSQFVWL 217

BLAST of Cmc04g0117531 vs. ExPASy TrEMBL
Match: A0A6J1IAR7 (Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111472844 PE=3 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 1.4e-53
Identity = 106/215 (49.30%), Postives = 139/215 (64.65%), Query Frame = 0

Query: 6   LVIYFLSLVLVVNAGVPFDFFQFVQQWGLNVCNTGAHVSPCHTPPRPMFTIHGLWPSNHS 65
           +V  F  ++L +     FD+FQ VQQW    C +G     C+  P  MFTIHGLWPS +S
Sbjct: 8   IVSIFNLVLLTIGVKAQFDYFQMVQQWPPATC-SGVR---CYANPPAMFTIHGLWPSTYS 67

Query: 66  NAHLVCWSKT-QYSKVISLEAQLKIYWPDLISGNNDKFWGHEWDNHGKCSDPPFGLFQYF 125
           N   VC  +    +++ +LE QL  YWPD+I+GNN +FW HEWDNHG CSDPPF   QYF
Sbjct: 68  NVPPVCAGQPFDRAQIATLEPQLNTYWPDVINGNNQRFWKHEWDNHGTCSDPPFNQLQYF 127

Query: 126 QISLNLR--KQFDLLAILTAAGLNPQSSNDLVIQDIITPIQKITKTEPGIRCNKNGRTGK 185
           Q +LN+R    +DLLAIL  AGL P ++     Q I   IQ  T  +PG+RCNKN ++ +
Sbjct: 128 QTTLNIRTNHNYDLLAILNTAGLGPTATTTRQYQAIEGAIQAATGKKPGLRCNKNAQSKR 187

Query: 186 LQLYEIVLCLEKDGVTLIDCPSFVSGTCPTKFVWL 218
            QL+EI+LC +K+GVTLI+C  F   TCP++FVWL
Sbjct: 188 KQLFEIILCFDKNGVTLINCTQFAGITCPSQFVWL 218

BLAST of Cmc04g0117531 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 132.9 bits (333), Expect = 3.3e-31
Identity = 74/211 (35.07%), Postives = 113/211 (53.55%), Query Frame = 0

Query: 6   LVIYFLSLVLVVNAGVPFDFFQFVQQWGLNVCNTGAHVS-PCHTPPRPMFTIHGLWPSNH 65
           L++   S+    ++   FDFF FVQQW  + C+T      P    P   F IHGLWP+  
Sbjct: 13  LLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGLWPNYK 72

Query: 66  SNAH-LVCWSKTQY--SKVISLEAQLKIYWPDLI--SGNNDKFWGHEWDNHGKCSDPPFG 125
              +   C +   +  S +  L   +K  WP L   SG+ + FW HEW+ HG CS+    
Sbjct: 73  DGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVID 132

Query: 126 LFQYFQISLNLRKQFDLLAILTAAGLNPQSSNDLVIQDIITPIQKITKTEPGIRCNKNGR 185
             +YFQ +LNL+++ +LL  LT AG+NP   +   ++ I   I++     P + CN++G 
Sbjct: 133 QHEYFQTALNLKQKTNLLGALTKAGINPDGKS-YSLESIRDSIKESIGFTPWVECNRDG- 192

Query: 186 TGKLQLYEIVLCLEKDGVTLIDCPSFVSGTC 211
           +G  QLY++ LC+++ G  LI+CP F  G C
Sbjct: 193 SGNSQLYQVYLCVDRSGSGLIECPVFPHGKC 221

BLAST of Cmc04g0117531 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 121.3 bits (303), Expect = 1.0e-27
Identity = 70/197 (35.53%), Postives = 103/197 (52.28%), Query Frame = 0

Query: 23  FDFFQFVQQWGLNVCNTG-AHVSPCHTPPRPMFTIHGLWPS-NHSNAHLVCWSKTQY--S 82
           FDFF FV QW    C+T  A   P    P   F IHGLWP+ N  +    C   +Q+  S
Sbjct: 28  FDFFYFVLQWPGAYCDTSRACCYPTSGKPAADFGIHGLWPNYNGGSWPSNCDPDSQFDRS 87

Query: 83  KVISLEAQLKIYWPDLISGNND--KFWGHEWDNHGKCSDPPFGLFQYFQISLNLRKQFDL 142
           ++  L + LK  WP L   +N+   FW HEW+ HG CS+      +YF+ +L L+++ +L
Sbjct: 88  QISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEKHGTCSESVMDQHEYFENALKLKQKANL 147

Query: 143 LAILTAAGLNPQSSNDLVIQDIITPIQKITKTEPGIRCNKNGRTGKLQLYEIVLCLEKDG 202
           L IL  +G+NP       +  I   I+      PGI CNK+      QL++I +C++  G
Sbjct: 148 LQILKNSGINP-DDGFYNLDKITNAIKDGIGFTPGIECNKDPER-NAQLHQIYICVDTSG 207

Query: 203 VTLIDCPSFVSGTCPTK 214
              I+CP    G+CP++
Sbjct: 208 TEFIECPVLPRGSCPSQ 222

BLAST of Cmc04g0117531 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 113.6 bits (283), Expect = 2.1e-25
Identity = 71/218 (32.57%), Postives = 106/218 (48.62%), Query Frame = 0

Query: 6   LVIYFLSLVLVVNAGVPFDFFQFVQQWGLNVCNT-GAHVSPCHTPPRPMFTIHGLWPSNH 65
           + I  L  + V +    FDFF FV QW    C++  +   P    P   F IHGLWP+  
Sbjct: 5   IFILALQQLYVQSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPNYK 64

Query: 66  SNAHLVCW-------SKTQYSKVISLEAQLKIYWPDLISGNND--KFWGHEWDNHGKCSD 125
           +      W       S+    +V  L + L+  WP L   +ND  KFW HEW+ HG C++
Sbjct: 65  TGG----WPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAE 124

Query: 126 PPFGLFQYFQISLNLRKQFDLLAILTAAGLNPQSSNDLVIQDIITPIQKITKTEPGIRCN 185
                  YF+  L L+++ +LL  LT AG+ P       ++DI   I+++    PGI CN
Sbjct: 125 SELDQHDYFEAGLKLKQKANLLHALTNAGIKP-DDKFYEMKDIENTIKQVVGFAPGIECN 184

Query: 186 KNGRTGKLQLYEIVLCLEKDGVTLIDCPSFVSGTCPTK 214
           K+  +   QLY+I LC++      I+CP    G C ++
Sbjct: 185 KDS-SHNSQLYQIYLCVDTSASKFINCPVMPHGRCDSR 216

BLAST of Cmc04g0117531 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 92.8 bits (229), Expect = 3.8e-19
Identity = 57/188 (30.32%), Postives = 84/188 (44.68%), Query Frame = 0

Query: 23  FDFFQFVQQWGLNVCNTGAHV----SPCHTPPRP-MFTIHGLWPSNHSNAHLVCWSKTQY 82
           FD+F    QW    C    H     + C     P  FTIHGLWP  +  +   C  ++ +
Sbjct: 32  FDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQFTIHGLWPDYNDGSWPSCCYRSDF 91

Query: 83  --SKVISLEAQLKIYWPDL-------ISGNNDKFWGHEWDNHGKCSDPPF-GLFQYFQIS 142
              ++ +L   L+ YWP L        +G    FWGHEW+ HG CS P F   + YF  +
Sbjct: 92  KEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHEWEKHGTCSSPVFHDEYNYFLTT 151

Query: 143 LNLRKQFDLLAILTAAGLNPQSSNDLVIQDIITPIQKITKTEPGIRCNKNGRTGKLQLYE 196
           LNL  + ++  +L  AG    +S    +  I+T IQ      P + C ++       + E
Sbjct: 152 LNLYLKHNVTDVLYQAGYVASNSEKYPLGGIVTAIQNAFHITPEVVCKRDA------IDE 211

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAE8651058.12.1e-9673.80hypothetical protein Csa_002465 [Cucumis sativus][more]
XP_031738690.16.2e-9677.31ribonuclease MC-like [Cucumis sativus][more]
XP_031738547.11.6e-8871.76ribonuclease MC-like [Cucumis sativus] >XP_031739193.1 ribonuclease MC-like [Cuc... [more]
XP_011651927.12.1e-6756.12ribonuclease MC [Cucumis sativus] >KGN58920.1 hypothetical protein Csa_001884 [C... [more]
TYK25090.12.6e-6252.99S-RNase [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
P428134.7e-3035.07Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1[more]
P800224.0e-2935.65Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2[more]
P235402.0e-2835.35Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1[more]
O803231.7e-2433.49Ribonuclease S-3 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1[more]
P428152.9e-2432.57Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LAN63.0e-9677.31Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G745530 PE=3 SV=1[more]
A0A0A0LAB01.0e-6756.12Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G736680 PE=3 SV=1[more]
A0A5D3DP911.3e-6252.99Ribonuclease T(2) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00... [more]
A0A6J1EXD51.3e-5450.23Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111439251 PE=3 SV=1[more]
A0A6J1IAR71.4e-5349.30Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111472844 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G02990.13.3e-3135.07ribonuclease 1 [more]
AT1G14220.11.0e-2735.53Ribonuclease T2 family protein [more]
AT1G26820.12.1e-2532.57ribonuclease 3 [more]
AT2G39780.13.8e-1930.32ribonuclease 2 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036430Ribonuclease T2-like superfamilyGENE3D3.90.730.10coord: 18..226
e-value: 3.0E-49
score: 169.8
IPR036430Ribonuclease T2-like superfamilySUPERFAMILY55895Ribonuclease Rh-likecoord: 18..216
IPR001568Ribonuclease T2-likePFAMPF00445Ribonuclease_T2coord: 23..202
e-value: 4.9E-40
score: 137.6
IPR001568Ribonuclease T2-likePANTHERPTHR11240RIBONUCLEASE T2coord: 11..212
NoneNo IPR availablePANTHERPTHR11240:SF49RIBONUCLEASE 3coord: 11..212
IPR018188Ribonuclease T2, His active site 1PROSITEPS00530RNASE_T2_1coord: 54..61
IPR033697Ribonuclease T2, eukaryoticCDDcd01061RNase_T2_eukcoord: 23..216
e-value: 8.26821E-55
score: 172.132

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc04g0117531.1Cmc04g0117531.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016070 RNA metabolic process
cellular_component GO:0110165 cellular anatomical entity
molecular_function GO:0033897 ribonuclease T2 activity
molecular_function GO:0003723 RNA binding