Cmc02g0048101 (gene) Melon (Charmono) v1.1

Overview
NameCmc02g0048101
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionHydroxymethylbilane hydrolyase [cyclizing]
LocationCMiso1.1chr02: 13652794 .. 13653973 (+)
RNA-Seq ExpressionCmc02g0048101
SyntenyCmc02g0048101
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGTGTGAACTCCAAAGAAATCACAAATGAGCACCGGCGCCGCGCATCCCAACGGCCCATTTCACATCAGTCCACTCACTTCATCTCCTTCTCCCGCCATCCCCACCCACTCCTCTCCGCGTACGGTGGCCTTCACGACGCCTCAAAACTATGCCGGCAGCCTCTCTCACCTCCTCTCGCTCAAAGGCTTCGAACCCCTCTGGTGTCCCACTCTTACCGTCCAGCCCACTCCTCTCGCCATCAAATCTCATCTCCTTCCGCCCATTCTGCATTCCTTCTCCGCCGTCGCCTTCACTTCCCGCTCCGGCATTACAGCCCTCCTCGACGCTGCTACTGAAATTGGCGAGCCCTTGCTACCGTCGCACGGCGATACTTTCTTAATCGCAGCCCTAGGTAAGGACTCCGAGCTTCTCGACCATGAATTTCTTACCACAATTTGCCCTAACACGAGTCGAATTAGAGTTGTGGTACCTGAAATTGCAACTCCAACTGGTTTAGTTGAAGCTCTTGGAGTTGGAAACCACCGTCGGGTTTTATGTCCGGTTCCTCGCGTCGTGGGACTCAACGAACCCCCGGTGGTTCCAAACTTCCTCCGTGACCTGGAGGCGAAAGGTTGGGTTCCGGTCCGCGTCGATGCTTACGAGACCCGATGGGCCGGACCCGATTGCGCGAGAAAGCTGGTGGAGAGAGGGAAGGATGAGAAATTGGATGCCATTGTGTTTACAAGTACTGGGGAAGTGGAGGGGCTGCTTAAAAGCTTGGAGCATTTGGGTTTGGAGTGGGATATGATGAAAAAGAGATGGCCGGAAATGGTGGTGGCTGCGCATGGGCCCGTGACAGCGGCAGGAGCTGAGAGACTCGGCGTTAAGGTTGATTTGGTGAGTCCTAAGTTTGATAGCTTCAATGGTGTGGTTGATTCTCTTCATTGGAGATGGCAGAGCTTAGATTAGAACACTTTCTAAATTCTTAAACATTTCAAGTTCTTTACGGTAACTTTAATCGCCATTTTATATATGCACTTGAAGCTGAATAATCTTCTTGTATACAATAATTTAGAAGGAATCGTTTCATCTTTATTCATTCAAACCAGCGCTTTGCTTTTGAATTGCTTTGGTTATCTTCACCATTTGTATCATTCTTTTAAGAATATCATTCTTTTAATACAAAAATTTATTAGTTT

mRNA sequence

GAGTGTGAACTCCAAAGAAATCACAAATGAGCACCGGCGCCGCGCATCCCAACGGCCCATTTCACATCAGTCCACTCACTTCATCTCCTTCTCCCGCCATCCCCACCCACTCCTCTCCGCGTACGGTGGCCTTCACGACGCCTCAAAACTATGCCGGCAGCCTCTCTCACCTCCTCTCGCTCAAAGGCTTCGAACCCCTCTGGTGTCCCACTCTTACCGTCCAGCCCACTCCTCTCGCCATCAAATCTCATCTCCTTCCGCCCATTCTGCATTCCTTCTCCGCCGTCGCCTTCACTTCCCGCTCCGGCATTACAGCCCTCCTCGACGCTGCTACTGAAATTGGCGAGCCCTTGCTACCGTCGCACGGCGATACTTTCTTAATCGCAGCCCTAGGTAAGGACTCCGAGCTTCTCGACCATGAATTTCTTACCACAATTTGCCCTAACACGAGTCGAATTAGAGTTGTGGTACCTGAAATTGCAACTCCAACTGGTTTAGTTGAAGCTCTTGGAGTTGGAAACCACCGTCGGGTTTTATGTCCGGTTCCTCGCGTCGTGGGACTCAACGAACCCCCGGTGGTTCCAAACTTCCTCCGTGACCTGGAGGCGAAAGGTTGGGTTCCGGTCCGCGTCGATGCTTACGAGACCCGATGGGCCGGACCCGATTGCGCGAGAAAGCTGGTGGAGAGAGGGAAGGATGAGAAATTGGATGCCATTGTGTTTACAAGTACTGGGGAAGTGGAGGGGCTGCTTAAAAGCTTGGAGCATTTGGGTTTGGAGTGGGATATGATGAAAAAGAGATGGCCGGAAATGGTGGTGGCTGCGCATGGGCCCGTGACAGCGGCAGGAGCTGAGAGACTCGGCGTTAAGGTTGATTTGGTGAGTCCTAAGTTTGATAGCTTCAATGGTGTGGTTGATTCTCTTCATTGGAGATGGCAGAGCTTAGATTAGAACACTTTCTAAATTCTTAAACATTTCAAGTTCTTTACGGTAACTTTAATCGCCATTTTATATATGCACTTGAAGCTGAATAATCTTCTTGTATACAATAATTTAGAAGGAATCGTTTCATCTTTATTCATTCAAACCAGCGCTTTGCTTTTGAATTGCTTTGGTTATCTTCACCATTTGTATCATTCTTTTAAGAATATCATTCTTTTAATACAAAAATTTATTAGTTT

Coding sequence (CDS)

ATGAGCACCGGCGCCGCGCATCCCAACGGCCCATTTCACATCAGTCCACTCACTTCATCTCCTTCTCCCGCCATCCCCACCCACTCCTCTCCGCGTACGGTGGCCTTCACGACGCCTCAAAACTATGCCGGCAGCCTCTCTCACCTCCTCTCGCTCAAAGGCTTCGAACCCCTCTGGTGTCCCACTCTTACCGTCCAGCCCACTCCTCTCGCCATCAAATCTCATCTCCTTCCGCCCATTCTGCATTCCTTCTCCGCCGTCGCCTTCACTTCCCGCTCCGGCATTACAGCCCTCCTCGACGCTGCTACTGAAATTGGCGAGCCCTTGCTACCGTCGCACGGCGATACTTTCTTAATCGCAGCCCTAGGTAAGGACTCCGAGCTTCTCGACCATGAATTTCTTACCACAATTTGCCCTAACACGAGTCGAATTAGAGTTGTGGTACCTGAAATTGCAACTCCAACTGGTTTAGTTGAAGCTCTTGGAGTTGGAAACCACCGTCGGGTTTTATGTCCGGTTCCTCGCGTCGTGGGACTCAACGAACCCCCGGTGGTTCCAAACTTCCTCCGTGACCTGGAGGCGAAAGGTTGGGTTCCGGTCCGCGTCGATGCTTACGAGACCCGATGGGCCGGACCCGATTGCGCGAGAAAGCTGGTGGAGAGAGGGAAGGATGAGAAATTGGATGCCATTGTGTTTACAAGTACTGGGGAAGTGGAGGGGCTGCTTAAAAGCTTGGAGCATTTGGGTTTGGAGTGGGATATGATGAAAAAGAGATGGCCGGAAATGGTGGTGGCTGCGCATGGGCCCGTGACAGCGGCAGGAGCTGAGAGACTCGGCGTTAAGGTTGATTTGGTGAGTCCTAAGTTTGATAGCTTCAATGGTGTGGTTGATTCTCTTCATTGGAGATGGCAGAGCTTAGATTAG

Protein sequence

MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWCPTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIAALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLNEPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEGLLKSLEHLGLEWDMMKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLHWRWQSLD
Homology
BLAST of Cmc02g0048101 vs. NCBI nr
Match: XP_008444040.1 (PREDICTED: uncharacterized protein LOC103487492 [Cucumis melo] >KAA0050031.1 Tetrapyrrole biosynthesis, uroporphyrinogen III synthase [Cucumis melo var. makuwa])

HSP 1 Score: 628.6 bits (1620), Expect = 2.8e-176
Identity = 307/307 (100.00%), Postives = 307/307 (100.00%), Query Frame = 0

Query: 1   MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWC 60
           MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWC
Sbjct: 1   MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWC 60

Query: 61  PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA 120
           PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA
Sbjct: 61  PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA 120

Query: 121 ALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLN 180
           ALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLN
Sbjct: 121 ALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLEHLGLEWDMMKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLH 300
           LLKSLEHLGLEWDMMKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLH
Sbjct: 241 LLKSLEHLGLEWDMMKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLH 300

Query: 301 WRWQSLD 308
           WRWQSLD
Sbjct: 301 WRWQSLD 307

BLAST of Cmc02g0048101 vs. NCBI nr
Match: TYK03635.1 (Tetrapyrrole biosynthesis, uroporphyrinogen III synthase [Cucumis melo var. makuwa])

HSP 1 Score: 627.1 bits (1616), Expect = 8.0e-176
Identity = 306/307 (99.67%), Postives = 307/307 (100.00%), Query Frame = 0

Query: 1   MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWC 60
           MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWC
Sbjct: 1   MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWC 60

Query: 61  PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA 120
           PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA
Sbjct: 61  PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA 120

Query: 121 ALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLN 180
           ALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLN
Sbjct: 121 ALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLEHLGLEWDMMKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLH 300
           LLKSLEHLGLEWD+MKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLH
Sbjct: 241 LLKSLEHLGLEWDIMKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLH 300

Query: 301 WRWQSLD 308
           WRWQSLD
Sbjct: 301 WRWQSLD 307

BLAST of Cmc02g0048101 vs. NCBI nr
Match: XP_011648476.1 (uncharacterized protein LOC105434481 [Cucumis sativus] >KGN65221.1 hypothetical protein Csa_019699 [Cucumis sativus])

HSP 1 Score: 599.7 bits (1545), Expect = 1.4e-167
Identity = 292/307 (95.11%), Postives = 299/307 (97.39%), Query Frame = 0

Query: 1   MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWC 60
           MSTGAAHPNGPFH++ LTSSPSPAIPTH SPRT AFTTP NYAGSLSHLLSLKGFEPLWC
Sbjct: 1   MSTGAAHPNGPFHLNSLTSSPSPAIPTHFSPRTAAFTTPPNYAGSLSHLLSLKGFEPLWC 60

Query: 61  PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA 120
           PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA
Sbjct: 61  PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA 120

Query: 121 ALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLN 180
           ALGKDSELLDHEFLTTIC NTSRIRVVVPEIATPTGLVEALGVGNHR VLCPVPRVVGLN
Sbjct: 121 ALGKDSELLDHEFLTTICHNTSRIRVVVPEIATPTGLVEALGVGNHRSVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLEHLGLEWDMMKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLH 300
           LLKSL HLGLEWD+MK+RWPEMVVAAHGPVTAAGAERLGVKVDLVS KFDSFNGVVD+LH
Sbjct: 241 LLKSLAHLGLEWDVMKRRWPEMVVAAHGPVTAAGAERLGVKVDLVSSKFDSFNGVVDALH 300

Query: 301 WRWQSLD 308
           WRWQ+L+
Sbjct: 301 WRWQNLE 307

BLAST of Cmc02g0048101 vs. NCBI nr
Match: XP_038878808.1 (uncharacterized protein LOC120070948 [Benincasa hispida] >XP_038878809.1 uncharacterized protein LOC120070948 [Benincasa hispida] >XP_038878810.1 uncharacterized protein LOC120070948 [Benincasa hispida])

HSP 1 Score: 566.2 bits (1458), Expect = 1.7e-157
Identity = 276/307 (89.90%), Postives = 290/307 (94.46%), Query Frame = 0

Query: 1   MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWC 60
           MSTGAAHPN P H++PL+ SPS AIPTHS+ RTVAFTTPQNYA SLSHLLSLK FEPLWC
Sbjct: 1   MSTGAAHPNRPIHLNPLSLSPSAAIPTHSTRRTVAFTTPQNYADSLSHLLSLKSFEPLWC 60

Query: 61  PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA 120
           PTLTV PTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPS GDTFLIA
Sbjct: 61  PTLTVHPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSRGDTFLIA 120

Query: 121 ALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLN 180
           ALGKDSELLDH FL+  CPNTSRIRVVVPEIATPTGLVEALG+GN RRVLCPVPRVVGLN
Sbjct: 121 ALGKDSELLDHGFLSRFCPNTSRIRVVVPEIATPTGLVEALGIGNQRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDLE KGWVPVRV AYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLETKGWVPVRVAAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLEHLGLEWDMMKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLH 300
           LLKSL HLGL+W++M+KRWPEMVVAA+GPVTAAGAERLG+KVDLVS KFDSFNGVVD+LH
Sbjct: 241 LLKSLGHLGLKWEVMRKRWPEMVVAANGPVTAAGAERLGIKVDLVSSKFDSFNGVVDALH 300

Query: 301 WRWQSLD 308
           WRWQSL+
Sbjct: 301 WRWQSLE 307

BLAST of Cmc02g0048101 vs. NCBI nr
Match: KAG6587847.1 (hypothetical protein SDJN03_16412, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035661.1 hypothetical protein SDJN02_02459, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 529.6 bits (1363), Expect = 1.7e-146
Identity = 258/307 (84.04%), Postives = 281/307 (91.53%), Query Frame = 0

Query: 1   MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWC 60
           MSTGAAHP+   H++PL SSPSPAIPT+S  RTVAFTTPQNYAGSLS+LLSLKGF+PLWC
Sbjct: 1   MSTGAAHPSPIHHLNPLASSPSPAIPTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC 60

Query: 61  PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA 120
           PT+TV PTPLAIKSHLLPP LH +SAVAFTSRSGITALLDAATEI EPLL   GDTFLIA
Sbjct: 61  PTVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDEPLLSPQGDTFLIA 120

Query: 121 ALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLN 180
           ALGKDSELLDH  L+  CPN SRIRVVVP+IA+P+GLVEALG+GNHRRVLCPVPRVVGLN
Sbjct: 121 ALGKDSELLDHGVLSKFCPNASRIRVVVPKIASPSGLVEALGIGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDL A GWVPVRVDAYETRW GP+CARKL ERG+DEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLAASGWVPVRVDAYETRWVGPECARKLAERGEDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLEHLGLEWDMMKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLH 300
           LLKSL  LGL+W+MM+K+WPEMV+AAHGPVTAAGAERLGVK+DLVS KFDSFNGVVD+LH
Sbjct: 241 LLKSLRALGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH 300

Query: 301 WRWQSLD 308
            RWQSL+
Sbjct: 301 SRWQSLE 307

BLAST of Cmc02g0048101 vs. ExPASy Swiss-Prot
Match: Q8KCJ3 (Uroporphyrinogen-III synthase OS=Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) OX=194439 GN=hemD PE=3 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 2.0e-04
Identity = 56/263 (21.29%), Postives = 108/263 (41.06%), Query Frame = 0

Query: 32  RTVAFTTPQNYAGSLSHLLSLKGFEPLWCPTLTVQPTPLAIKSHLLPPILHSFSAVAFTS 91
           +TV  T P++ A      L+  G + +  PT+ ++P      +    P L  F+ + FTS
Sbjct: 2   KTVLVTRPKHQAEPFVRELAQYGLDSVVFPTIEIRPV-----TGWSVPDLTRFAGIFFTS 61

Query: 92  RSGITALLDAATEIGEPLLPSHGDTFLIAALGKDSELLDHEFLTTICPNTSRIRVVVPEI 151
            + +   L+   E     LP+      + A+GK +     +   +I P        +P+ 
Sbjct: 62  PNSVQFFLERLLEESPDELPNLQQA-RVWAVGKTTGGDLEKHGVSIEP--------LPKS 121

Query: 152 ATPTGLVEALGVGN-HRRVLCPVPRVVGLNEPPVVPNFLRDLEAKGWVPVRVDAYETRWA 211
           A    L+  +       +    V   + L   P V      +  +G + V +  Y+    
Sbjct: 122 ADAVSLMSGIDASEIEGKTFLFVRGSLSLGTIPEV------IAKRGGICVELTVYDNIQP 181

Query: 212 GPDCARKLVERGKDEKLDAIVFTSTGEVEGLLKSLEHLGLEWDMMKKRWPEMVVAAHGPV 271
             +  +K+     + K+D + FTS        ++++         K+   ++++AA G  
Sbjct: 182 SLEETQKIKSLLTEGKIDCLSFTSPSTAINFFEAID--------SKEVPSDVLIAAIGTT 236

Query: 272 TAAGAERLGVKVDLVSPKFDSFN 294
           T++  E+LGVKVD++   FD  N
Sbjct: 242 TSSALEKLGVKVDIIPEYFDGPN 236

BLAST of Cmc02g0048101 vs. ExPASy TrEMBL
Match: A0A5A7U8V3 (Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00050 PE=3 SV=1)

HSP 1 Score: 628.6 bits (1620), Expect = 1.3e-176
Identity = 307/307 (100.00%), Postives = 307/307 (100.00%), Query Frame = 0

Query: 1   MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWC 60
           MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWC
Sbjct: 1   MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWC 60

Query: 61  PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA 120
           PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA
Sbjct: 61  PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA 120

Query: 121 ALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLN 180
           ALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLN
Sbjct: 121 ALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLEHLGLEWDMMKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLH 300
           LLKSLEHLGLEWDMMKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLH
Sbjct: 241 LLKSLEHLGLEWDMMKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLH 300

Query: 301 WRWQSLD 308
           WRWQSLD
Sbjct: 301 WRWQSLD 307

BLAST of Cmc02g0048101 vs. ExPASy TrEMBL
Match: A0A1S3BA78 (Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis melo OX=3656 GN=LOC103487492 PE=3 SV=1)

HSP 1 Score: 628.6 bits (1620), Expect = 1.3e-176
Identity = 307/307 (100.00%), Postives = 307/307 (100.00%), Query Frame = 0

Query: 1   MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWC 60
           MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWC
Sbjct: 1   MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWC 60

Query: 61  PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA 120
           PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA
Sbjct: 61  PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA 120

Query: 121 ALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLN 180
           ALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLN
Sbjct: 121 ALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLEHLGLEWDMMKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLH 300
           LLKSLEHLGLEWDMMKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLH
Sbjct: 241 LLKSLEHLGLEWDMMKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLH 300

Query: 301 WRWQSLD 308
           WRWQSLD
Sbjct: 301 WRWQSLD 307

BLAST of Cmc02g0048101 vs. ExPASy TrEMBL
Match: A0A5D3BX13 (Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1369G00630 PE=3 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 3.9e-176
Identity = 306/307 (99.67%), Postives = 307/307 (100.00%), Query Frame = 0

Query: 1   MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWC 60
           MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWC
Sbjct: 1   MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWC 60

Query: 61  PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA 120
           PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA
Sbjct: 61  PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA 120

Query: 121 ALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLN 180
           ALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLN
Sbjct: 121 ALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLEHLGLEWDMMKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLH 300
           LLKSLEHLGLEWD+MKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLH
Sbjct: 241 LLKSLEHLGLEWDIMKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLH 300

Query: 301 WRWQSLD 308
           WRWQSLD
Sbjct: 301 WRWQSLD 307

BLAST of Cmc02g0048101 vs. ExPASy TrEMBL
Match: A0A0A0LZ08 (Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis sativus OX=3659 GN=Csa_1G266180 PE=3 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 6.7e-168
Identity = 292/307 (95.11%), Postives = 299/307 (97.39%), Query Frame = 0

Query: 1   MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWC 60
           MSTGAAHPNGPFH++ LTSSPSPAIPTH SPRT AFTTP NYAGSLSHLLSLKGFEPLWC
Sbjct: 1   MSTGAAHPNGPFHLNSLTSSPSPAIPTHFSPRTAAFTTPPNYAGSLSHLLSLKGFEPLWC 60

Query: 61  PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA 120
           PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA
Sbjct: 61  PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA 120

Query: 121 ALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLN 180
           ALGKDSELLDHEFLTTIC NTSRIRVVVPEIATPTGLVEALGVGNHR VLCPVPRVVGLN
Sbjct: 121 ALGKDSELLDHEFLTTICHNTSRIRVVVPEIATPTGLVEALGVGNHRSVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLEHLGLEWDMMKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLH 300
           LLKSL HLGLEWD+MK+RWPEMVVAAHGPVTAAGAERLGVKVDLVS KFDSFNGVVD+LH
Sbjct: 241 LLKSLAHLGLEWDVMKRRWPEMVVAAHGPVTAAGAERLGVKVDLVSSKFDSFNGVVDALH 300

Query: 301 WRWQSLD 308
           WRWQ+L+
Sbjct: 301 WRWQNLE 307

BLAST of Cmc02g0048101 vs. ExPASy TrEMBL
Match: A0A6J1KXQ2 (Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucurbita maxima OX=3661 GN=LOC111497286 PE=3 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 3.2e-146
Identity = 259/307 (84.36%), Postives = 283/307 (92.18%), Query Frame = 0

Query: 1   MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWC 60
           MSTGAAHP+   H++PL SSPSPAIPT+S  RTVAFTTPQNYAGSLS+LLSLKGF+PLWC
Sbjct: 1   MSTGAAHPS-LIHLNPLASSPSPAIPTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC 60

Query: 61  PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA 120
           PT+TV PTPLAIKSHLLPP LH +SAVAFTSRSGITALLDAATEI +PLL   GDTFLIA
Sbjct: 61  PTVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDDPLLSPQGDTFLIA 120

Query: 121 ALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLN 180
           ALGKDSELLDH  L+  CPNTSRIRVVVP+IATP+GLVEALG+GNHRRVLCPVPRVVGLN
Sbjct: 121 ALGKDSELLDHGVLSKFCPNTSRIRVVVPKIATPSGLVEALGIGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDL A GWVPVRVDAYETRWAGP+CAR LV+RG+DEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLAASGWVPVRVDAYETRWAGPECARMLVKRGEDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLEHLGLEWDMMKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLH 300
           LLKSL  LGL+W+MM+K+WPEMV+AAHGPVTAAGAERLGVK+DLVS KFDSFNGVVD+LH
Sbjct: 241 LLKSLRTLGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH 300

Query: 301 WRWQSLD 308
            RWQSL+
Sbjct: 301 SRWQSLE 306

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008444040.12.8e-176100.00PREDICTED: uncharacterized protein LOC103487492 [Cucumis melo] >KAA0050031.1 Tet... [more]
TYK03635.18.0e-17699.67Tetrapyrrole biosynthesis, uroporphyrinogen III synthase [Cucumis melo var. maku... [more]
XP_011648476.11.4e-16795.11uncharacterized protein LOC105434481 [Cucumis sativus] >KGN65221.1 hypothetical ... [more]
XP_038878808.11.7e-15789.90uncharacterized protein LOC120070948 [Benincasa hispida] >XP_038878809.1 unchara... [more]
KAG6587847.11.7e-14684.04hypothetical protein SDJN03_16412, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
Q8KCJ32.0e-0421.29Uroporphyrinogen-III synthase OS=Chlorobaculum tepidum (strain ATCC 49652 / DSM ... [more]
Match NameE-valueIdentityDescription
A0A5A7U8V31.3e-176100.00Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3BA781.3e-176100.00Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis melo OX=3656 GN=LOC1034874... [more]
A0A5D3BX133.9e-17699.67Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis melo var. makuwa OX=119469... [more]
A0A0A0LZ086.7e-16895.11Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis sativus OX=3659 GN=Csa_1G2... [more]
A0A6J1KXQ23.2e-14684.36Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003754Tetrapyrrole biosynthesis, uroporphyrinogen III synthasePFAMPF02602HEM4coord: 46..293
e-value: 2.7E-26
score: 92.3
IPR003754Tetrapyrrole biosynthesis, uroporphyrinogen III synthaseCDDcd06578HemDcoord: 34..299
e-value: 6.88125E-27
score: 103.541
IPR036108Tetrapyrrole biosynthesis, uroporphyrinogen III synthase superfamilyGENE3D3.40.50.10090coord: 185..303
e-value: 3.3E-14
score: 54.7
coord: 28..163
e-value: 3.4E-6
score: 28.8
IPR036108Tetrapyrrole biosynthesis, uroporphyrinogen III synthase superfamilySUPERFAMILY69618HemD-likecoord: 32..290
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..31
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 13..31
NoneNo IPR availablePANTHERPTHR38020UROPORPHYRINOGEN-III SYNTHASEcoord: 17..305

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc02g0048101.1Cmc02g0048101.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006782 protoporphyrinogen IX biosynthetic process
biological_process GO:0033014 tetrapyrrole biosynthetic process
molecular_function GO:0004852 uroporphyrinogen-III synthase activity