Cmc01g0032861 (gene) Melon (Charmono) v1.1

Overview
NameCmc01g0032861
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Description4-coumarate--CoA ligase
LocationCMiso1.1chr01: 33174554 .. 33176605 (-)
RNA-Seq ExpressionCmc01g0032861
SyntenyCmc01g0032861
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCAAATTACAAATATATATATATATATATATATGTCCAATTTATTTTTCGAACCAACCATACACTAATTTTCTACTATTTTCTGATTTCTATAAAATCCCTTATCTTCTTTATTCAAATTCAAACCATACCCATCTCTACATTTTCTCTTCTCTCTGTTTCTACTCTGTTTTTCTTCAAACCTCAACAATGGCATTCGAATCAAACGAAACTAATGAATTCATTTTCCGTTCAAAGCTTCCCGATATTCACATTCCCAATCATCTTCCACTCCACGATTATGTTTTCCAAAATCTCTCCAAATTCGCCTCACGCCCCTGTTTGATCAATGGCGCCACCAGGGATGTTTACACCTACCATGACGTTCAGCTAACAGCCCGTCGAGTCGCCGCTGGCCTACACAACCTCGGTATTAAGAAGGGTGACGTTGTCATGAATTTACTTCCCAATTCCCCGGAGTTCGTCTTCACCTTCCTTGGCGCGTCTTACCGGGGCGCCATCATGACGGCAGCCAATCCTTTTTACACGGCTGTGGAAATAGCTAAACAGGCGAAAGCCGCCGATGCGAAATTAATCGTTACAATGGCTTGTTTTTATGATCGCGTTAAGGATTTGGGTGAAAATGGTGTCAAAATCGTGTGTGTTGATTTTGCCGTGGAGGGTTGTTTGCATTTCTCTGTTTTGAGTGGGGCTGATGAATCTCTTGCGCCGCCGGTGGATTTTTCCTCTGATGACGTGGTGGCATTACCGTACTCCTCCGGCACTACCGGTTTGCCGAAGGGAGTTATGCTGACACATAAAGGGTTAATCACAAGTGTGGCTCAACAAATGGATGGCCAAAATCCAAATCTCTATTATCACAGCAATGATGTTATCCTCTGTGTGCTGCCGTTTTTCCATATCTATTCACTCAATTCCATTTTGCTGTGTGGACTACGTGTCGGTTCTGCCATTTTGATTGTGCAAAAATTCGACATCGTTTCACTTTTACAATTGATTGAGAAACATAGAATCTCGATCATGCCCATCGTGCCGCCAATCTTTTTGGCCATCGCTAAGTCCCCAGAATTTGAAAAGTACGACGTGTCGTCCGTGAGGGTTCTAAAATCTGGTGGAGCACCACTGGGGAAGGAGTTGGAAGATGCCGTGAGGGAGAAATTTCCAACGGCGATTCTCGGACAGGGGTATGGAATGACTGAGGCAGGTCCAGTTCTGTCCATGAGCTTAGCTTTTGCGAAAGAACCGTTTCAAGTAAAAGCCGGAGCCTGTGGAACGGTGGTCCGCAATGCAGAGATGAAGATTGTTGACACCGAAACCGGCGCCTCATTGCCGGCGAATTCCTCCGGAGAAATTTGTATTAGAGGCGAACAAATCATGAAAGGATATTTGAATGATTTGGAGTCAACAAAGAGGACTATTGATAAAGAAGGATGGCTTCACACTGGCGACATTGGCTTTGTCGACAATGACAACGAGCTATTCATCGTTGATCGGCTTAAGGAACTAATAAAATTCAAGGCATTTCAAGTGGCTCCTGCTGAGCTCGAGGCCCTTCTCATCACACATCCTAAACTATCTGATGCTGCCGTCATTGGGTGAGTTCAAATGAATTTCATTCTTTATTTTAGCTAAAAATTATTTGTGGGAGTTTCACTTCTAATCAAATTTTATTTTTCTTACTGTTTTTTAGTATGCCGGATGTGGAGGCGGGAGAAGTGCCGGTGGCGTTTGTGGTAAAGGCGATCGGCGGTGCAATAACAGAAGAAGAAGTAAAGCAATTCATAACAAAACAAGTTGTTTTCTACAAAAGACTAAAACGTGTGCTTTTCGTTAACGCTATTCCAAAGGCTCCATCGGGCAAAATCCTTAGAAAGGAACTCAGAGCAAAACTTGCTTCCGGTGCTTACAATTAGTCACCCAATTTCTTCCTTTGTTGTCAATAGTATTAAGATTTTATATACTCTTTCATATGTATTATAATATTCAACATTATTTAAATGCATACTATGTATCATTCCATTACTAAATCTTAAATCACGAATTTATCTAAAGC

mRNA sequence

CCAAATTACAAATATATATATATATATATATATGTCCAATTTATTTTTCGAACCAACCATACACTAATTTTCTACTATTTTCTGATTTCTATAAAATCCCTTATCTTCTTTATTCAAATTCAAACCATACCCATCTCTACATTTTCTCTTCTCTCTGTTTCTACTCTGTTTTTCTTCAAACCTCAACAATGGCATTCGAATCAAACGAAACTAATGAATTCATTTTCCGTTCAAAGCTTCCCGATATTCACATTCCCAATCATCTTCCACTCCACGATTATGTTTTCCAAAATCTCTCCAAATTCGCCTCACGCCCCTGTTTGATCAATGGCGCCACCAGGGATGTTTACACCTACCATGACGTTCAGCTAACAGCCCGTCGAGTCGCCGCTGGCCTACACAACCTCGGTATTAAGAAGGGTGACGTTGTCATGAATTTACTTCCCAATTCCCCGGAGTTCGTCTTCACCTTCCTTGGCGCGTCTTACCGGGGCGCCATCATGACGGCAGCCAATCCTTTTTACACGGCTGTGGAAATAGCTAAACAGGCGAAAGCCGCCGATGCGAAATTAATCGTTACAATGGCTTGTTTTTATGATCGCGTTAAGGATTTGGGTGAAAATGGTGTCAAAATCGTGTGTGTTGATTTTGCCGTGGAGGGTTGTTTGCATTTCTCTGTTTTGAGTGGGGCTGATGAATCTCTTGCGCCGCCGGTGGATTTTTCCTCTGATGACGTGGTGGCATTACCGTACTCCTCCGGCACTACCGGTTTGCCGAAGGGAGTTATGCTGACACATAAAGGGTTAATCACAAGTGTGGCTCAACAAATGGATGGCCAAAATCCAAATCTCTATTATCACAGCAATGATGTTATCCTCTGTGTGCTGCCGTTTTTCCATATCTATTCACTCAATTCCATTTTGCTGTGTGGACTACGTGTCGGTTCTGCCATTTTGATTGTGCAAAAATTCGACATCGTTTCACTTTTACAATTGATTGAGAAACATAGAATCTCGATCATGCCCATCGTGCCGCCAATCTTTTTGGCCATCGCTAAGTCCCCAGAATTTGAAAAGTACGACGTGTCGTCCGTGAGGGTTCTAAAATCTGGTGGAGCACCACTGGGGAAGGAGTTGGAAGATGCCGTGAGGGAGAAATTTCCAACGGCGATTCTCGGACAGGGGTATGGAATGACTGAGGCAGGTCCAGTTCTGTCCATGAGCTTAGCTTTTGCGAAAGAACCGTTTCAAGTAAAAGCCGGAGCCTGTGGAACGGTGGTCCGCAATGCAGAGATGAAGATTGTTGACACCGAAACCGGCGCCTCATTGCCGGCGAATTCCTCCGGAGAAATTTGTATTAGAGGCGAACAAATCATGAAAGGATATTTGAATGATTTGGAGTCAACAAAGAGGACTATTGATAAAGAAGGATGGCTTCACACTGGCGACATTGGCTTTGTCGACAATGACAACGAGCTATTCATCGTTGATCGGCTTAAGGAACTAATAAAATTCAAGGCATTTCAAGTGGCTCCTGCTGAGCTCGAGGCCCTTCTCATCACACATCCTAAACTATCTGATGCTGCCGTCATTGGTATGCCGGATGTGGAGGCGGGAGAAGTGCCGGTGGCGTTTGTGGTAAAGGCGATCGGCGGTGCAATAACAGAAGAAGAAGTAAAGCAATTCATAACAAAACAAGTTGTTTTCTACAAAAGACTAAAACGTGTGCTTTTCGTTAACGCTATTCCAAAGGCTCCATCGGGCAAAATCCTTAGAAAGGAACTCAGAGCAAAACTTGCTTCCGGTGCTTACAATTAGTCACCCAATTTCTTCCTTTGTTGTCAATAGTATTAAGATTTTATATACTCTTTCATATGTATTATAATATTCAACATTATTTAAATGCATACTATGTATCATTCCATTACTAAATCTTAAATCACGAATTTATCTAAAGC

Coding sequence (CDS)

ATGGCATTCGAATCAAACGAAACTAATGAATTCATTTTCCGTTCAAAGCTTCCCGATATTCACATTCCCAATCATCTTCCACTCCACGATTATGTTTTCCAAAATCTCTCCAAATTCGCCTCACGCCCCTGTTTGATCAATGGCGCCACCAGGGATGTTTACACCTACCATGACGTTCAGCTAACAGCCCGTCGAGTCGCCGCTGGCCTACACAACCTCGGTATTAAGAAGGGTGACGTTGTCATGAATTTACTTCCCAATTCCCCGGAGTTCGTCTTCACCTTCCTTGGCGCGTCTTACCGGGGCGCCATCATGACGGCAGCCAATCCTTTTTACACGGCTGTGGAAATAGCTAAACAGGCGAAAGCCGCCGATGCGAAATTAATCGTTACAATGGCTTGTTTTTATGATCGCGTTAAGGATTTGGGTGAAAATGGTGTCAAAATCGTGTGTGTTGATTTTGCCGTGGAGGGTTGTTTGCATTTCTCTGTTTTGAGTGGGGCTGATGAATCTCTTGCGCCGCCGGTGGATTTTTCCTCTGATGACGTGGTGGCATTACCGTACTCCTCCGGCACTACCGGTTTGCCGAAGGGAGTTATGCTGACACATAAAGGGTTAATCACAAGTGTGGCTCAACAAATGGATGGCCAAAATCCAAATCTCTATTATCACAGCAATGATGTTATCCTCTGTGTGCTGCCGTTTTTCCATATCTATTCACTCAATTCCATTTTGCTGTGTGGACTACGTGTCGGTTCTGCCATTTTGATTGTGCAAAAATTCGACATCGTTTCACTTTTACAATTGATTGAGAAACATAGAATCTCGATCATGCCCATCGTGCCGCCAATCTTTTTGGCCATCGCTAAGTCCCCAGAATTTGAAAAGTACGACGTGTCGTCCGTGAGGGTTCTAAAATCTGGTGGAGCACCACTGGGGAAGGAGTTGGAAGATGCCGTGAGGGAGAAATTTCCAACGGCGATTCTCGGACAGGGGTATGGAATGACTGAGGCAGGTCCAGTTCTGTCCATGAGCTTAGCTTTTGCGAAAGAACCGTTTCAAGTAAAAGCCGGAGCCTGTGGAACGGTGGTCCGCAATGCAGAGATGAAGATTGTTGACACCGAAACCGGCGCCTCATTGCCGGCGAATTCCTCCGGAGAAATTTGTATTAGAGGCGAACAAATCATGAAAGGATATTTGAATGATTTGGAGTCAACAAAGAGGACTATTGATAAAGAAGGATGGCTTCACACTGGCGACATTGGCTTTGTCGACAATGACAACGAGCTATTCATCGTTGATCGGCTTAAGGAACTAATAAAATTCAAGGCATTTCAAGTGGCTCCTGCTGAGCTCGAGGCCCTTCTCATCACACATCCTAAACTATCTGATGCTGCCGTCATTGGTATGCCGGATGTGGAGGCGGGAGAAGTGCCGGTGGCGTTTGTGGTAAAGGCGATCGGCGGTGCAATAACAGAAGAAGAAGTAAAGCAATTCATAACAAAACAAGTTGTTTTCTACAAAAGACTAAAACGTGTGCTTTTCGTTAACGCTATTCCAAAGGCTCCATCGGGCAAAATCCTTAGAAAGGAACTCAGAGCAAAACTTGCTTCCGGTGCTTACAATTAG

Protein sequence

MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATRDVYTYHDVQLTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAADAKLIVTMACFYDRVKDLGENGVKIVCVDFAVEGCLHFSVLSGADESLAPPVDFSSDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPFFHIYSLNSILLCGLRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVSSVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGACGTVVRNAEMKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGWLHTGDIGFVDNDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPVAFVVKAIGGAITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRKELRAKLASGAYN
Homology
BLAST of Cmc01g0032861 vs. NCBI nr
Match: XP_008461217.1 (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis melo])

HSP 1 Score: 1061.6 bits (2744), Expect = 2.3e-306
Identity = 540/542 (99.63%), Postives = 541/542 (99.82%), Query Frame = 0

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATRDVYTYHDVQ 60
           MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGAT DVYTYHDVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVM+LLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMHLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAADAKLIVTMACFYDRVKDLGENGVKIVCVDFAVEGCLHFSVLSGADESLAPPVDFSS 180
           AKAADAKLIVTMACFYDRVKDLGENGVKIVCVDFAVEGCLHFSVLSGADESLAPPVDFSS
Sbjct: 121 AKAADAKLIVTMACFYDRVKDLGENGVKIVCVDFAVEGCLHFSVLSGADESLAPPVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFVDNDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IGFVDNDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDNDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKAIGGAITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRKELRAKLASGA 540
           AFVVKAIGGAITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKAIGGAITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of Cmc01g0032861 vs. NCBI nr
Match: KAA0058679.1 (4-coumarate--CoA ligase 2-like [Cucumis melo var. makuwa] >TYK10485.1 4-coumarate--CoA ligase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 1054.3 bits (2725), Expect = 3.6e-304
Identity = 538/542 (99.26%), Postives = 539/542 (99.45%), Query Frame = 0

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATRDVYTYHDVQ 60
           MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGAT DVYTYHDVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVM+LLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMHLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAADAKLIVTMACFYDRVKDLGENGVKIVCVDFAVEGCLHFSVLSGADESLAPPVDFSS 180
           AKAADAKLIVTMACFYDRVKDLGENGVKIVCVDFAVEGCLHFSVLSGADESLA PVDFSS
Sbjct: 121 AKAADAKLIVTMACFYDRVKDLGENGVKIVCVDFAVEGCLHFSVLSGADESLALPVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFVDNDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IGFVDNDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDNDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKAIGGAITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRKELRAKLASGA 540
           AFVVKAIG AITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKAIGVAITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of Cmc01g0032861 vs. NCBI nr
Match: XP_004135894.2 (4-coumarate--CoA ligase 2 [Cucumis sativus] >KGN45189.1 hypothetical protein Csa_016278 [Cucumis sativus])

HSP 1 Score: 1033.9 bits (2672), Expect = 5.0e-298
Identity = 524/542 (96.68%), Postives = 535/542 (98.71%), Query Frame = 0

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATRDVYTYHDVQ 60
           MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGAT DVYTYHDVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAADAKLIVTMACFYDRVKDLGENGVKIVCVDFAVEGCLHFSVLSGADESLAPPVDFSS 180
           AKAA+AKLIVTMACFYDRVKDL ENGV+IVCVDFAVEGCLHFSVLSGADESLAP VDFSS
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVQIVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPFFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYH NDVILCVLPFFHIYS
Sbjct: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLRVG+AI+I+QKFDIV+LLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDTETGASLPANSSGEICIRG+QIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFVDNDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IGFVD+DNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKAIGGAITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRKELRAKLASGA 540
           AFV+KA GGAI+EEEVKQFI KQVVFYKRLKRV FVNAIPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVMKANGGAISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of Cmc01g0032861 vs. NCBI nr
Match: XP_004135893.3 (4-coumarate--CoA ligase 2 [Cucumis sativus] >KAE8646417.1 hypothetical protein Csa_015838 [Cucumis sativus])

HSP 1 Score: 1028.9 bits (2659), Expect = 1.6e-296
Identity = 521/542 (96.13%), Postives = 535/542 (98.71%), Query Frame = 0

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATRDVYTYHDVQ 60
           MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGAT DVYTYHDVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAADAKLIVTMACFYDRVKDLGENGVKIVCVDFAVEGCLHFSVLSGADESLAPPVDFSS 180
           AKAA+AKLIVTMACFYDRVKDL ENGV++VCVDFAVEGCLHFSVLSGADESLAP VDFSS
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPFFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDG+NPNLYYH++DVIL VLPFFHIYS
Sbjct: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240

Query: 241 LNSILLCGLRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLRVG+AILI+QKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFP A+LGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDTETGASLPANSSGEICIRG+QIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFVDNDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IGFVD+D+ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKAIGGAITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRKELRAKLASGA 540
           AFVVKA GGAITEEEVKQFITKQVVFYKRLKRV FVNAIPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of Cmc01g0032861 vs. NCBI nr
Match: KAA0058682.1 (4-coumarate--CoA ligase 1-like [Cucumis melo var. makuwa] >TYK10488.1 4-coumarate--CoA ligase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1021.9 bits (2641), Expect = 2.0e-294
Identity = 517/542 (95.39%), Postives = 531/542 (97.97%), Query Frame = 0

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATRDVYTYHDVQ 60
           MAFESN+T EFIFRSKLPDIHIPNHLPLH YVFQN+ KFASRPCLINGAT DVYTYHDVQ
Sbjct: 1   MAFESNQTQEFIFRSKLPDIHIPNHLPLHQYVFQNMPKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAADAKLIVTMACFYDRVKDLGENGVKIVCVDFAVEGCLHFSVLSGADESLAPPVDFSS 180
           AKAA+AKLIVTMACFYDRVKDL ENGVKIVCVDFAVEGCLHFSVLSGADESLAP VDFSS
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADESLAPVVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDG+NPNLYYH++DVIL VLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240

Query: 241 LNSILLCGLRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLRVG+ ILI+QKFDIVSLLQLIEKH+ISIMPIVPPIFLAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRVGATILIMQKFDIVSLLQLIEKHKISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFPTA+LGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDTETGASLPANSSGEICIRG+QIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFVDNDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IGFVD+D+ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKAIGGAITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRKELRAKLASGA 540
           AFVVKA GGAITEEEVKQFITKQVVFYKRLKRV FVNAIPKAPSGKILRKELRAKLA GA
Sbjct: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLALGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of Cmc01g0032861 vs. ExPASy Swiss-Prot
Match: O24146 (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)

HSP 1 Score: 792.7 bits (2046), Expect = 2.6e-228
Identity = 389/534 (72.85%), Postives = 458/534 (85.77%), Query Frame = 0

Query: 10  EFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATRDVYTYHDVQLTARRVAAG 69
           + IFRSKLPDI+IPNHLPLH Y F+N+S+F+SRPCLINGA + +YTY DV+L +R+VAAG
Sbjct: 9   DIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELNSRKVAAG 68

Query: 70  LHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAADAKLI 129
           LH  GI+  D +M LLPNSPEFVF F+GASY GAI T ANP +T  E+ KQAKA+ AK+I
Sbjct: 69  LHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKII 128

Query: 130 VTMACFYDRVKDLG-ENGVKIVCVDFAVEGCLHFSVLSGADESLAPPVDFSSDDVVALPY 189
           VT AC  ++VKD   EN VKI+C+D A EGCLHFSVL+ A+E   P V+   DDVVALPY
Sbjct: 129 VTQACHVNKVKDYAFENDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEIQPDDVVALPY 188

Query: 190 SSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPFFHIYSLNSILLCG 249
           SSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY HS DV+LCVLP FHIYSLNS+LLCG
Sbjct: 189 SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIYSLNSVLLCG 248

Query: 250 LRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVSSVRVLKSG 309
           LRVG+AILI+QKFDIVS L+LI++++++I P VPPI LAIAKSP  + YD+SSVR + SG
Sbjct: 249 LRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDLSSVRTVMSG 308

Query: 310 GAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGACGTVVRNAE 369
            APLGKELED VR KFP A LGQGYGMTEAGPVL+M LAFAKEPF++K+GACGTVVRNAE
Sbjct: 309 AAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVRNAE 368

Query: 370 MKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGWLHTGDIGFVDNDN 429
           MKIVD +TG SLP N SGEICIRG+QIMKGYLND E+T RTIDKEGWL+TGDIG++D+D+
Sbjct: 369 MKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTGDIGYIDDDD 428

Query: 430 ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPVAFVVKAIG 489
           ELFIVDRLKELIK+K FQVAPAELEALL+ HP +SDAAV+ M D +AGEVPVAFVV++ G
Sbjct: 429 ELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSNG 488

Query: 490 GAITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRKELRAKLASGAYN 543
             ITE+EVK FI+KQV+FYKR+KRV FV+AIPK+PSGKILRK+LRAKLA+G  N
Sbjct: 489 STITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAGLPN 542

BLAST of Cmc01g0032861 vs. ExPASy Swiss-Prot
Match: O24145 (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 785.0 bits (2026), Expect = 5.3e-226
Identity = 383/540 (70.93%), Postives = 453/540 (83.89%), Query Frame = 0

Query: 4   ESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATRDVYTYHDVQLTA 63
           E+ ++ + IFRSKLPDI+IP HLPLH Y F+N+S+F+SRPCLINGA   +YTY +V+LT 
Sbjct: 8   ETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTC 67

Query: 64  RRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKA 123
           R+VA GL+ LGI++ D +M LLPNSPEFVF F+GASY GAI T ANP +T  E+ KQAKA
Sbjct: 68  RKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKA 127

Query: 124 ADAKLIVTMACFYDRVKD-LGENGVKIVCVDFAVEGCLHFSVLSGADESLAPPVDFSSDD 183
           + AK+I+T +CF  +VKD   EN VK++C+D A EGCLHFS L+ +DE   P V    DD
Sbjct: 128 SSAKIIITQSCFVGKVKDYASENDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQPDD 187

Query: 184 VVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPFFHIYSLN 243
           VVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+N NLY HS DV++CVLP FHIYSLN
Sbjct: 188 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLN 247

Query: 244 SILLCGLRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVSSV 303
           SILLCGLRVG+AILI+QKFDI   L+LI+K+++SI P VPPI LAIAKSP  + YD+SSV
Sbjct: 248 SILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSSV 307

Query: 304 RVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGACGT 363
           R + SG APLGKELEDAVR KFP A LGQGYGMTEAGPVL+M LAFAKEPF +K+GACGT
Sbjct: 308 RTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGT 367

Query: 364 VVRNAEMKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGWLHTGDIG 423
           VVRNAEMKIVD +TG SLP N  GEICIRG+QIMKGYLND E+T RTIDKEGWLHTGDIG
Sbjct: 368 VVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIG 427

Query: 424 FVDNDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPVAF 483
           F+D D+ELFIVDRLKELIK+K FQVAPAE+EALL+ HP +SDAAV+ M D +AGEVPVAF
Sbjct: 428 FIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAF 487

Query: 484 VVKAIGGAITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRKELRAKLASGAYN 543
           VV++ G AITE+EVK FI+KQV+FYKR+KRV FV  +PK+PSGKILRK+LRA+LA+G  N
Sbjct: 488 VVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAGVPN 547

BLAST of Cmc01g0032861 vs. ExPASy Swiss-Prot
Match: I3PB37 (4-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1)

HSP 1 Score: 784.6 bits (2025), Expect = 7.0e-226
Identity = 384/543 (70.72%), Postives = 457/543 (84.16%), Query Frame = 0

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATRDVYTYHDVQ 60
           M  E+N+  + IFRSKLPDI+IP HLPLH Y F+N+S+F+SRPCLINGA   +YTY DV+
Sbjct: 3   METETNQ-GDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANNHIYTYADVE 62

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LT+R+VAAGL+ LGI++ D +M LLPNSPEFVF F+GASY GAI T ANP +T  E+ KQ
Sbjct: 63  LTSRKVAAGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AKAADAKLIVTMACFYDRVKDLG-ENGVKIVCVDFAVEGCLHFSVLSGADESLAPPVDFS 180
           AKA++AKLI+T ACF ++VKD   +N + ++C+D A EGC+HFS L+ ADE   P V   
Sbjct: 123 AKASNAKLIITQACFVNKVKDYAFDNNLNVICIDSAPEGCIHFSELTQADEHDIPDVKIQ 182

Query: 181 SDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPFFHIY 240
           SDDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+N NLY HS DV++CVLP FHIY
Sbjct: 183 SDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIY 242

Query: 241 SLNSILLCGLRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDV 300
           SLNS+LLCGLRVG+AILI+QKFDIV   +LIEK++++I P VPPI LAIAKSP  + YD+
Sbjct: 243 SLNSVLLCGLRVGAAILIMQKFDIVQFCELIEKYKVTIGPFVPPIVLAIAKSPVVDNYDL 302

Query: 301 SSVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGA 360
           SSVR + SG APLGKELEDAVR KFP A LGQGYGMTEAGPVL+M LAFAKEPF +K+GA
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRIKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGWLHTG 420
           CGTVVRNAEMKIVD +TG SLP N  GEICIRG+QIMKGYLND  +T RTIDKEGWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPAATTRTIDKEGWLHTG 422

Query: 421 DIGFVDNDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVP 480
           DIG++DND+ELFIVDRLKELIK+K FQVAPAELEALL+ HP +SDAAV+ M D +AGEVP
Sbjct: 423 DIGYIDNDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVP 482

Query: 481 VAFVVKAIGGAITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRKELRAKLASG 540
           VAFVV++ G  ITE+EVK F++KQV+FYKR+KRV FV  +PK+PSGKILRK+LRA+LA+G
Sbjct: 483 VAFVVRSNGSDITEDEVKDFVSKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 542

Query: 541 AYN 543
             N
Sbjct: 543 VPN 544

BLAST of Cmc01g0032861 vs. ExPASy Swiss-Prot
Match: P31685 (4-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1)

HSP 1 Score: 774.6 bits (1999), Expect = 7.2e-223
Identity = 380/543 (69.98%), Postives = 450/543 (82.87%), Query Frame = 0

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATRDVYTYHDVQ 60
           M  E+ ++ + IFRSKLPDI+IP HLPLH Y F+NLS+F SRPCLI+GA   +YTY +V+
Sbjct: 3   MDIETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVE 62

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LT+R+VA GL+ LGI++ D +M LLPN PEFVF F+GASY GAI T ANP +T  E+ KQ
Sbjct: 63  LTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AKAADAKLIVTMACFYDRVKDLG-ENGVKIVCVDFAVEGCLHFSVLSGADESLAPPVDFS 180
           AKA+ AK+++T ACF  +VKD   EN +K++CVD A EGC+HFS L  +DE   P V   
Sbjct: 123 AKASSAKIVITQACFAGKVKDYAIENDLKVICVDSAPEGCVHFSELIQSDEHEIPDVKIQ 182

Query: 181 SDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPFFHIY 240
            DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+N NLY HS+DV++CVLP FHIY
Sbjct: 183 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIY 242

Query: 241 SLNSILLCGLRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDV 300
           SLNS+LLC LRVG+AILI+QKFDI   L+LI KH+++I P VPPI LAIAKSP    YD+
Sbjct: 243 SLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVHNYDL 302

Query: 301 SSVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGA 360
           SSVR + SG APLGKELEDAVR KFP A LGQGYGMTEAGPVL+M LAFAKEPF +K+GA
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGWLHTG 420
           CGTVVRNAEMKIVD +TG SLP N  GEICIRG+QIMKGYLND E+T RTI+KEGWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTG 422

Query: 421 DIGFVDNDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVP 480
           DIGF+D+D+ELFIVDRLKELIK+K FQVAPAELEALLI HP +SDAAV+ M D +AGEVP
Sbjct: 423 DIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVP 482

Query: 481 VAFVVKAIGGAITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRKELRAKLASG 540
           VAFVV++ G  ITE+EVK FI+KQV+FYKR+KRV FV  +PK+PSGKILRK+LRA+LA+G
Sbjct: 483 VAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 542

Query: 541 AYN 543
             N
Sbjct: 543 ISN 545

BLAST of Cmc01g0032861 vs. ExPASy Swiss-Prot
Match: O24540 (4-coumarate--CoA ligase OS=Vanilla planifolia OX=51239 GN=4CL PE=3 SV=1)

HSP 1 Score: 774.6 bits (1999), Expect = 7.2e-223
Identity = 385/540 (71.30%), Postives = 455/540 (84.26%), Query Frame = 0

Query: 7   ETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATRDVYTYHDVQLTARRV 66
           +  + IFRSKLPDI+IP +LPLH Y F+N+SKF+SRPCLINGAT +++TY DV+L +RRV
Sbjct: 10  QKKDIIFRSKLPDIYIPKNLPLHSYCFENISKFSSRPCLINGATDEIFTYADVELISRRV 69

Query: 67  AAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAADA 126
            +GL  LGIK+GD +M LLPNSPEFVF FLGAS+ G+I T ANPF+T+ E+ KQAKA++A
Sbjct: 70  GSGLSKLGIKQGDTIMILLPNSPEFVFAFLGASFIGSISTMANPFFTSTEVIKQAKASNA 129

Query: 127 KLIVTMACFYDRVKDLG-ENGVKIVCVDFA-----VEGCLHFSVLSGADESLAPPVDFSS 186
           KLI+T  C+ D+VKD   ENGVKI+ +D           LHFS L+GADE+  P V+ S 
Sbjct: 130 KLIITQGCYVDKVKDYACENGVKIISIDTTTTADDAANILHFSELTGADENEMPKVEISP 189

Query: 187 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPFFHIYS 246
           D VVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY HS+DV+LCVLP FHIYS
Sbjct: 190 DGVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYMHSDDVLLCVLPLFHIYS 249

Query: 247 LNSILLCGLRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 306
           LNS+LLCGLR GS ILI+QKF+IV  L+LI+K++++I P VPPI LAIAKS   + YD+S
Sbjct: 250 LNSVLLCGLRAGSGILIMQKFEIVPFLELIQKYKVTIGPFVPPIVLAIAKSTVVDNYDLS 309

Query: 307 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 366
           SVR + SG APLGKELEDAVR KFP A LGQGYGMTEAGPVL+M LAFAKEPF +K+GAC
Sbjct: 310 SVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGAC 369

Query: 367 GTVVRNAEMKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGWLHTGD 426
           GTVVRNAEMKIVD ETG+SLP N  GEICIRG+QIMKGYLND E+T RTIDKEGWLHTGD
Sbjct: 370 GTVVRNAEMKIVDPETGSSLPRNHPGEICIRGDQIMKGYLNDPEATARTIDKEGWLHTGD 429

Query: 427 IGFVDNDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 486
           IG++D+D+ELFIVDRLKELIK+K FQVAPAELEALL+THP +SDAAV+ M D  AGEVPV
Sbjct: 430 IGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPCISDAAVVPMKDEAAGEVPV 489

Query: 487 AFVVKAIGGAITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRKELRAKLASGA 541
           AFVVK+ G  ITE+E+KQFI+KQV+FYKR+ RV FV AIPKAPSGKILRK+LRA+LA+ A
Sbjct: 490 AFVVKSNGHNITEDEIKQFISKQVIFYKRINRVFFVEAIPKAPSGKILRKDLRARLAAAA 549

BLAST of Cmc01g0032861 vs. ExPASy TrEMBL
Match: A0A1S3CEM1 (4-coumarate--CoA ligase 2-like OS=Cucumis melo OX=3656 GN=LOC103499868 PE=4 SV=1)

HSP 1 Score: 1061.6 bits (2744), Expect = 1.1e-306
Identity = 540/542 (99.63%), Postives = 541/542 (99.82%), Query Frame = 0

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATRDVYTYHDVQ 60
           MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGAT DVYTYHDVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVM+LLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMHLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAADAKLIVTMACFYDRVKDLGENGVKIVCVDFAVEGCLHFSVLSGADESLAPPVDFSS 180
           AKAADAKLIVTMACFYDRVKDLGENGVKIVCVDFAVEGCLHFSVLSGADESLAPPVDFSS
Sbjct: 121 AKAADAKLIVTMACFYDRVKDLGENGVKIVCVDFAVEGCLHFSVLSGADESLAPPVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFVDNDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IGFVDNDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDNDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKAIGGAITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRKELRAKLASGA 540
           AFVVKAIGGAITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKAIGGAITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of Cmc01g0032861 vs. ExPASy TrEMBL
Match: A0A5A7UYT0 (4-coumarate--CoA ligase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001350 PE=4 SV=1)

HSP 1 Score: 1054.3 bits (2725), Expect = 1.7e-304
Identity = 538/542 (99.26%), Postives = 539/542 (99.45%), Query Frame = 0

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATRDVYTYHDVQ 60
           MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGAT DVYTYHDVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVM+LLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMHLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAADAKLIVTMACFYDRVKDLGENGVKIVCVDFAVEGCLHFSVLSGADESLAPPVDFSS 180
           AKAADAKLIVTMACFYDRVKDLGENGVKIVCVDFAVEGCLHFSVLSGADESLA PVDFSS
Sbjct: 121 AKAADAKLIVTMACFYDRVKDLGENGVKIVCVDFAVEGCLHFSVLSGADESLALPVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFVDNDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IGFVDNDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDNDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKAIGGAITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRKELRAKLASGA 540
           AFVVKAIG AITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKAIGVAITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of Cmc01g0032861 vs. ExPASy TrEMBL
Match: A0A0A0K8H9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G430240 PE=4 SV=1)

HSP 1 Score: 1033.9 bits (2672), Expect = 2.4e-298
Identity = 524/542 (96.68%), Postives = 535/542 (98.71%), Query Frame = 0

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATRDVYTYHDVQ 60
           MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGAT DVYTYHDVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAADAKLIVTMACFYDRVKDLGENGVKIVCVDFAVEGCLHFSVLSGADESLAPPVDFSS 180
           AKAA+AKLIVTMACFYDRVKDL ENGV+IVCVDFAVEGCLHFSVLSGADESLAP VDFSS
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVQIVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPFFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYH NDVILCVLPFFHIYS
Sbjct: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLRVG+AI+I+QKFDIV+LLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDTETGASLPANSSGEICIRG+QIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFVDNDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IGFVD+DNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKAIGGAITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRKELRAKLASGA 540
           AFV+KA GGAI+EEEVKQFI KQVVFYKRLKRV FVNAIPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVMKANGGAISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of Cmc01g0032861 vs. ExPASy TrEMBL
Match: A0A0A0K658 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G430250 PE=4 SV=1)

HSP 1 Score: 1026.9 bits (2654), Expect = 3.0e-296
Identity = 520/542 (95.94%), Postives = 534/542 (98.52%), Query Frame = 0

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATRDVYTYHDVQ 60
           MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGAT D YTYHDVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDFYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAADAKLIVTMACFYDRVKDLGENGVKIVCVDFAVEGCLHFSVLSGADESLAPPVDFSS 180
           AKAA+AKLIVTMACFYDRVKDL ENGV++VCVDFAVEGCLHFSVLSGADESLAP VDFSS
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPFFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDG+NPNLYYH++DVIL VLPFFHIYS
Sbjct: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240

Query: 241 LNSILLCGLRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLRVG+AILI+QKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFP A+LGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDTETGASLPANSSGEICIRG+QIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFVDNDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IGFVD+D+ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKAIGGAITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRKELRAKLASGA 540
           AFVVKA GGAITEEEVKQFITKQVVFYKRLKRV FVNAIPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of Cmc01g0032861 vs. ExPASy TrEMBL
Match: A0A5A7US61 (4-coumarate--CoA ligase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001380 PE=4 SV=1)

HSP 1 Score: 1021.9 bits (2641), Expect = 9.6e-295
Identity = 517/542 (95.39%), Postives = 531/542 (97.97%), Query Frame = 0

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATRDVYTYHDVQ 60
           MAFESN+T EFIFRSKLPDIHIPNHLPLH YVFQN+ KFASRPCLINGAT DVYTYHDVQ
Sbjct: 1   MAFESNQTQEFIFRSKLPDIHIPNHLPLHQYVFQNMPKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAADAKLIVTMACFYDRVKDLGENGVKIVCVDFAVEGCLHFSVLSGADESLAPPVDFSS 180
           AKAA+AKLIVTMACFYDRVKDL ENGVKIVCVDFAVEGCLHFSVLSGADESLAP VDFSS
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADESLAPVVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDG+NPNLYYH++DVIL VLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240

Query: 241 LNSILLCGLRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLRVG+ ILI+QKFDIVSLLQLIEKH+ISIMPIVPPIFLAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRVGATILIMQKFDIVSLLQLIEKHKISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFPTA+LGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDTETGASLPANSSGEICIRG+QIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFVDNDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IGFVD+D+ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKAIGGAITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRKELRAKLASGA 540
           AFVVKA GGAITEEEVKQFITKQVVFYKRLKRV FVNAIPKAPSGKILRKELRAKLA GA
Sbjct: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLALGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of Cmc01g0032861 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 737.6 bits (1903), Expect = 7.0e-213
Identity = 364/539 (67.53%), Postives = 452/539 (83.86%), Query Frame = 0

Query: 8   TNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATRDVYTYHDVQLTARRVA 67
           +N+ IFRS+LPDI+IPNHLPLHDY+F+N+S+FA++PCLING T +VYTY DV +T+R++A
Sbjct: 18  SNDVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 77

Query: 68  AGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAADAK 127
           AGLHNLG+K+ DVVM LLPNSPE V TFL AS+ GAI T+ANPF+T  EI+KQAKA+ AK
Sbjct: 78  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 137

Query: 128 LIVTMACFYDRVKDLGENGVKIVCVDFAV--EGCLHFSVLSGADESLAP--PVDFSSDDV 187
           LIVT + + D++K+L  +GV IV  D     E CL FS L+ ++E      P   S +DV
Sbjct: 138 LIVTQSRYVDKIKNLQNDGVLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPEKISPEDV 197

Query: 188 VALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPFFHIYSLNS 247
           VALP+SSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY++ +DVILCVLP FHIY+LNS
Sbjct: 198 VALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYALNS 257

Query: 248 ILLCGLRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVSSVR 307
           I+LC LRVG+ ILI+ KF+I  LL+ I++ ++++  +VPPI LAIAKSPE EKYD+SSVR
Sbjct: 258 IMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSVR 317

Query: 308 VLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGACGTV 367
           ++KSG APLGKELEDA+  KFP A LGQGYGMTEAGPVL+MSL FAKEPF VK+GACGTV
Sbjct: 318 MVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTV 377

Query: 368 VRNAEMKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGWLHTGDIGF 427
           VRNAEMKI+D +TG SLP N  GEICIRG QIMKGYLND  +T  TIDK+GWLHTGD+GF
Sbjct: 378 VRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGF 437

Query: 428 VDNDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPVAFV 487
           +D+D+ELFIVDRLKELIK+K FQVAPAELE+LLI HP+++D AV+ M + +AGEVPVAFV
Sbjct: 438 IDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAFV 497

Query: 488 VKAIGGAITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRKELRAKLASGAYN 543
           V++    I+E+E+KQF++KQVVFYKR+ +V F ++IPKAPSGKILRK+LRA+LA+G  N
Sbjct: 498 VRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLANGLMN 556

BLAST of Cmc01g0032861 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 735.7 bits (1898), Expect = 2.6e-212
Identity = 368/544 (67.65%), Postives = 447/544 (82.17%), Query Frame = 0

Query: 5   SNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATRDVYTYHDVQLTAR 64
           +N  ++ IFRSKLPDI+IPNHL LHDY+FQN+S+FA++PCLING T  VYTY DV + +R
Sbjct: 17  NNNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISR 76

Query: 65  RVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAA 124
           ++AA  H LG+ + DVVM LLPN PEFV +FL AS+RGA  TAANPF+T  EIAKQAKA+
Sbjct: 77  QIAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKAS 136

Query: 125 DAKLIVTMACFYDRVKDL-GENGVKIVCVDF-----AVEGCLHFSVLSGADESLAPPVD- 184
           + KLI+T A + D++K L  ++GV IVC+D        EGCL F+ L+ +    +  +D 
Sbjct: 137 NTKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDS 196

Query: 185 --FSSDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPF 244
              S DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY+HS+DVILCVLP 
Sbjct: 197 VEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPM 256

Query: 245 FHIYSLNSILLCGLRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFE 304
           FHIY+LNSI+LCGLRVG+AILI+ KF+I  LL+LI++ ++++ P+VPPI LAIAKS E E
Sbjct: 257 FHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETE 316

Query: 305 KYDVSSVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQV 364
           KYD+SS+RV+KSG APLGKELEDAV  KFP A LGQGYGMTEAGPVL+MSL FAKEPF V
Sbjct: 317 KYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPV 376

Query: 365 KAGACGTVVRNAEMKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGW 424
           K+GACGTVVRNAEMKIVD +TG SL  N  GEICIRG QIMKGYLN+  +T  TIDK+GW
Sbjct: 377 KSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGW 436

Query: 425 LHTGDIGFVDNDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEA 484
           LHTGDIG +D+D+ELFIVDRLKELIK+K FQVAPAELEALLI HP ++D AV+ M +  A
Sbjct: 437 LHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAA 496

Query: 485 GEVPVAFVVKAIGGAITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRKELRAK 540
           GEVPVAFVVK+    ++E++VKQF++KQVVFYKR+ +V F  +IPKAPSGKILRK+LRAK
Sbjct: 497 GEVPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAK 556

BLAST of Cmc01g0032861 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 684.5 bits (1765), Expect = 7.0e-197
Identity = 342/509 (67.19%), Postives = 416/509 (81.73%), Query Frame = 0

Query: 5   SNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATRDVYTYHDVQLTAR 64
           +N  ++ IFRSKLPDI+IPNHL LHDY+FQN+S+FA++PCLING T  VYTY DV + +R
Sbjct: 17  NNNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISR 76

Query: 65  RVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAA 124
           ++AA  H LG+ + DVVM LLPN PEFV +FL AS+RGA  TAANPF+T  EIAKQAKA+
Sbjct: 77  QIAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKAS 136

Query: 125 DAKLIVTMACFYDRVKDL-GENGVKIVCVDF-----AVEGCLHFSVLSGADESLAPPVD- 184
           + KLI+T A + D++K L  ++GV IVC+D        EGCL F+ L+ +    +  +D 
Sbjct: 137 NTKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDS 196

Query: 185 --FSSDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPF 244
              S DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY+HS+DVILCVLP 
Sbjct: 197 VEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPM 256

Query: 245 FHIYSLNSILLCGLRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFE 304
           FHIY+LNSI+LCGLRVG+AILI+ KF+I  LL+LI++ ++++ P+VPPI LAIAKS E E
Sbjct: 257 FHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETE 316

Query: 305 KYDVSSVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQV 364
           KYD+SS+RV+KSG APLGKELEDAV  KFP A LGQGYGMTEAGPVL+MSL FAKEPF V
Sbjct: 317 KYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPV 376

Query: 365 KAGACGTVVRNAEMKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGW 424
           K+GACGTVVRNAEMKIVD +TG SL  N  GEICIRG QIMKGYLN+  +T  TIDK+GW
Sbjct: 377 KSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGW 436

Query: 425 LHTGDIGFVDNDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEA 484
           LHTGDIG +D+D+ELFIVDRLKELIK+K FQVAPAELEALLI HP ++D AV+ M +  A
Sbjct: 437 LHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAA 496

Query: 485 GEVPVAFVVKAIGGAITEEEVKQFITKQV 505
           GEVPVAFVVK+    ++E++VKQF++KQV
Sbjct: 497 GEVPVAFVVKSKDSELSEDDVKQFVSKQV 525

BLAST of Cmc01g0032861 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 677.2 bits (1746), Expect = 1.1e-194
Identity = 335/546 (61.36%), Postives = 427/546 (78.21%), Query Frame = 0

Query: 4   ESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLS----KFASRPCLINGATRDVYTYHDV 63
           E   +++FIFRSKLPDI IPNHLPL DYVFQ  S      +S  C+I+GAT  + TY DV
Sbjct: 20  EEEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADV 79

Query: 64  QLTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAK 123
           Q   RR+AAG+H LGI+ GDVVM LLPNSPEF  +FL  +Y GA+ T ANPFYT  EIAK
Sbjct: 80  QTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAK 139

Query: 124 QAKAADAKLIVTMACFYDRVKDLGENGVKIVCVD---------FAVEGCLHFSVLSGADE 183
           QAKA+ AK+I+T  C  D++ +L  +GV IVC+D          + +GC+ F+ L+ ADE
Sbjct: 140 QAKASAAKMIITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQADE 199

Query: 184 SLAPPVDFSSDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVIL 243
           +       S +D VA+PYSSGTTGLPKGVM+THKGL+TS+AQ++DG+NPNL + +NDVIL
Sbjct: 200 TELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVIL 259

Query: 244 CVLPFFHIYSLNSILLCGLRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAK 303
           C LP FHIY+L++++L  +R G+A+LIV +F++  +++LI++++++++P+ PP+ LA  K
Sbjct: 260 CFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFIK 319

Query: 304 SPEFEKYDVSSVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAK 363
           SPE E+YD+SSVR++ SG A L KELEDAVR KFP AI GQGYGMTE+G V + SLAFAK
Sbjct: 320 SPETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLAFAK 379

Query: 364 EPFQVKAGACGTVVRNAEMKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTI 423
            PF+ K+GACGTV+RNAEMK+VDTETG SLP N SGEIC+RG Q+MKGYLND E+T RTI
Sbjct: 380 NPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTI 439

Query: 424 DKEGWLHTGDIGFVDNDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGM 483
           DK+GWLHTGDIGFVD+D+E+FIVDRLKELIKFK +QVAPAELEALLI+HP + DAAV+ M
Sbjct: 440 DKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAM 499

Query: 484 PDVEAGEVPVAFVVKAIGGAITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRK 537
            D  A EVPVAFV ++ G  +TE++VK ++ KQVV YKR+K V F+  IPKA SGKILRK
Sbjct: 500 KDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKILRK 559

BLAST of Cmc01g0032861 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 666.4 bits (1718), Expect = 2.0e-191
Identity = 336/527 (63.76%), Postives = 411/527 (77.99%), Query Frame = 0

Query: 12  IFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATRDVYTYHDVQLTARRVAAGLH 71
           IFRSKLPDI IPNHLPLH Y F+ LS  + +PCLI G+T   YTY +  L  RRVA+GL+
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 72  NLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAADAKLIVT 131
            LGI+KGDV+M LL NS EFVF+F+GAS  GA+ T ANPFYT+ E+ KQ K++ AKLI+T
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 132 MACFYDRVKDLGENGVKIVCVDFAVEGCLHFSVLSGADES--LAPPVDFSSDDVVALPYS 191
            + + D++K+LGEN   I   +   E CL FS L   DE+      VD   DD  ALP+S
Sbjct: 154 HSQYVDKLKNLGENLTLITTDEPTPENCLPFSTLITDDETNPFQETVDIGGDDAAALPFS 213

Query: 192 SGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPFFHIYSLNSILLCGL 251
           SGTTGLPKGV+LTHK LITSVAQQ+DG NPNLY  SNDVILCVLP FHIYSLNS+LL  L
Sbjct: 214 SGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLNSL 273

Query: 252 RVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVSSVRVLKSGG 311
           R G+ +L++ KF+I +LL LI++HR++I  +VPP+ +A+AK+P    YD+SSVR + SG 
Sbjct: 274 RSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRFVLSGA 333

Query: 312 APLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGACGTVVRNAEM 371
           APLGKEL+D++R + P AILGQGYGMTEAGPVLSMSL FAKEP   K+G+CGTVVRNAE+
Sbjct: 334 APLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVRNAEL 393

Query: 372 KIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGWLHTGDIGFVDNDNE 431
           K+V  ET  SL  N  GEICIRG+QIMK YLND E+T  TID+EGWLHTGDIG+VD D+E
Sbjct: 394 KVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVDEDDE 453

Query: 432 LFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPVAFVVKAIGG 491
           +FIVDRLKE+IKFK FQV PAELE+LLI H  ++DAAV+   D  AGEVPVAFVV++ G 
Sbjct: 454 IFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVRSNGN 513

Query: 492 AITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRKELRAKL 537
            ITEE+VK+++ KQVVFYKRL +V FV +IPK+PSGKILRK+L+AKL
Sbjct: 514 DITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008461217.12.3e-30699.63PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis melo][more]
KAA0058679.13.6e-30499.264-coumarate--CoA ligase 2-like [Cucumis melo var. makuwa] >TYK10485.1 4-coumarat... [more]
XP_004135894.25.0e-29896.684-coumarate--CoA ligase 2 [Cucumis sativus] >KGN45189.1 hypothetical protein Csa... [more]
XP_004135893.31.6e-29696.134-coumarate--CoA ligase 2 [Cucumis sativus] >KAE8646417.1 hypothetical protein C... [more]
KAA0058682.12.0e-29495.394-coumarate--CoA ligase 1-like [Cucumis melo var. makuwa] >TYK10488.1 4-coumarat... [more]
Match NameE-valueIdentityDescription
O241462.6e-22872.854-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1[more]
O241455.3e-22670.934-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1[more]
I3PB377.0e-22670.724-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1[more]
P316857.2e-22369.984-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1[more]
O245407.2e-22371.304-coumarate--CoA ligase OS=Vanilla planifolia OX=51239 GN=4CL PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3CEM11.1e-30699.634-coumarate--CoA ligase 2-like OS=Cucumis melo OX=3656 GN=LOC103499868 PE=4 SV=1[more]
A0A5A7UYT01.7e-30499.264-coumarate--CoA ligase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A0A0K8H92.4e-29896.68Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G430240 PE=4 SV=1[more]
A0A0A0K6583.0e-29695.94Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G430250 PE=4 SV=1[more]
A0A5A7US619.6e-29595.394-coumarate--CoA ligase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
Match NameE-valueIdentityDescription
AT3G21240.17.0e-21367.534-coumarate:CoA ligase 2 [more]
AT1G51680.12.6e-21267.654-coumarate:CoA ligase 1 [more]
AT1G51680.37.0e-19767.194-coumarate:CoA ligase 1 [more]
AT3G21230.11.1e-19461.364-coumarate:CoA ligase 5 [more]
AT1G65060.12.0e-19163.764-coumarate:CoA ligase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 451..526
e-value: 4.7E-16
score: 59.5
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 3..436
e-value: 1.3E-165
score: 553.3
NoneNo IPR availableGENE3D3.30.300.30coord: 437..538
e-value: 5.7E-29
score: 102.8
NoneNo IPR availablePANTHERPTHR24096LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 7..537
NoneNo IPR availablePANTHERPTHR24096:SF3824-COUMARATE--COA LIGASE 2-LIKEcoord: 7..537
NoneNo IPR availableCDDcd059044CLcoord: 22..532
e-value: 0.0
score: 741.359
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 7..537
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 35..442
e-value: 3.7E-104
score: 348.7
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 186..197

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc01g0032861.1Cmc01g0032861.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016405 CoA-ligase activity