Cmc01g0003571 (gene) Melon (Charmono) v1.1

Overview


Name	Cmc01g0003571
Type	gene
Organism	Cucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Description	Cysteine protease
Location	CMiso1.1chr01: 519903 .. 522732 (+)
RNA-Seq Expression	Cmc01g0003571
Synteny	Cmc01g0003571

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAATATTCTCTGTAATTTGTGGACTTCTTTTTCCCAAATCCCATTTCTTCTTCTCCACTTCCCTATTTAAATTCTCCAATCCCCCCTTCACTTTTTCCTTCATACCCATTTCTTCTTCTCTCCCAAATCCCACAAAACATGGCGATTTCTTCACCCATTTTCTTCGCTTTTCTTGCCCTTTTCCTCTGTCTTTCCCCTTTTTCATCCGCTTCCCATTCATCCACCTTCTCCATCATTGATGAAAACGCTAAACACCACTTGGGTATCCCTGAAATTCCCCATTCCGATGCCCATCAACGCACCGACGAGGAAGTTGCCGCTTTGTACGAGTCCTGGTTGGTCCATCATGGAAAAGCCTACAATGCTCTTGGTGAGAAGGAGAGACGGTTTGAGATTTTTAAGGATAATCTCATGTTTATTGATGAACATAACCGGGAATCGCGGACGTATAAAGTTGGATTAACCCGTTTTGCTGATCTTACCAATGAGGAATATCGGGCAAGGTTTTTGGGTGGCCGATTCTCCCGGAAGCCTAGCTTATCCGCCGCCAAGAGCGGAAGGTACGCGGCGGCGCTCGGTGATGATCTTCCGGATGATGTCGATTGGAGAAAAAAGGGCGCTGTTGCTAATGTTAAAGATCAAGGACAGTGTGGTGAGTTTTCTTTTTATTTTTTCTCTCTCTTTTTTTTAAACTTTTAATTTTTTTTGTTGAATTGATCCCATGAGAATGATGCTTGGATTTTGTGTTTTCTTTTCGTTTTCTCATTTGGTCTTTGATATTTTGACTATTGTGTTTGGGTCGTTGCTGATTTTCCCAGGAAATTTGTTATGAGATCCATATTTATAGGTAACATTTCTCACAAATAGATTCATTAGATATTTGTCAATACTCAACCTTGATCGTAACTTGTATGTGTTATAATTAGCTTCGCTTCATCTCTGATGATTTGTTGATGGCCATCGATAACATCAGAAAACGTATTAGAAACTTTCCGCTCAAAACTTGTTTTCCAAGTAACCTTTTATCTCGAAGGATTGTTTTAGTAATGGCTTTTGATGGATGGCGATTGACGTAATTGGGTTATTTTTGGAATGAAGGGAGCTGTTGGGCTTTCTCAACAGTTGCTGCAGTGGAAGGAATAAACCAAATTGTCACTGGCGAATTGATCTCTCTGTCAGAGCAGGAACTTGTGGATTGTGACAAATCATTTAACATGGGTTGCAATGGTGGTCTTATGGACTATGCTTTCCAGTTCATCATTGACAATGGTGGAATTGACACCGACGAAGATTACCCTTACAAGGGACGAGATGGCGCTTGCGATCCCAATCGGGTTTGTCCCCAATCAACTTCACTTGATTCATTTATCTTACCATTTATCGTAATTTGACTGGTTTTTAATGTTTTGTCCAAAGAAAAATGCCAAGGTTGTAACCATAGATGGATATGAAGATGTTCCTGAGAATGATGAGAGCTCCTTGAAAAAGGCTGTAGCAAATCAACCAGTTAGTGTTGCAATTGAAGCTGGTGGCAGAGCCTTCCAACTTTACCAATCGGTAATCTCTTCAACTTTGAGCAATTTCCTGAATGTTGTTTCACAAAGCTAAGGTTTTGACTTCTCTGGCTTTGTAATGGTGGTATCTGTTTTGGAAGCTTATTCGAACATATTTTGTTGTGTGTTAATTCATCATTCTGGTTTGGGAATCAATATTCGAGTTTAATCTGTTGTGTTTGCGTGTCTTAATGACAAAGATAATCTGTATTGATCCACCATTTAGGCATGGGAAATGTTAGCTATTTCAAACACTTTGTCCTTATGCATCATATAAGGTGTTTTGTGTGTTTTAAATACAATATGTGCTTATGCATCAATTCAGGGTGTCTTCACTGGCCGTTGCGGAACCAATCTAGATCATGGTGTTGTTGCTGTTGGATATGGAACCGACAACGGTACAGATTATTGGATTGTGAGGAATTCATGGGGTAAAGACTGGGGAGAGAATGGTTATATCAGGTTGGAGAGAAATGTGGCCAACAGTACCACAGGCAAGTGTGGTATTGCAGTAGAGCCTTCATACCCCATCAAATCCGGATCAAACCCTCCAAAACCTTCTGCTTCTCCCCCATCTCCTGTCAACCCTCCAACTGAATGCGACGAATACTTCTCGTGCGATGAAGGAAGTACCTGCTGCTGTATCTATCAGTATGGAAGCACCTGCTTTGCTTGGGGATGCTGCCCCCTCGAGTCCGCAACCTGCTGTGATGACCATTACAGTTGCTGCCCACATGAATATCCCGTTTGTGACCTTGAGGCAGGAACTTGCCGAGCGGTACGTCACATCACATAGTTTTCTAACCAATGCTCATTCTTCAACGAGTTCTTTGTCATTCAAGTTCATGTTTGATATAATCTAATCATTGTAAATGGATGAAACACAGAGCAAAGACAGCTTGATGGGCGTCAATCTGCTGAAGCGCCTTCCTGCAAATCAGACAAAGCGGATTCAAAAGCTTGGGAAGTTGTTCGTTGGTGCTTGAGAGGTTTGGCAATGGTGGTCGGTGTCGTCAGTGAAATAATCGACCATTTGAACACAGGTCCATAGTATAGAAAAGTTATTAAGAAGTGGAAAAAGGTTGATGTCCCCATTTATTGATCTTTGATATTGTGGGGCTTATTATTTTCCTTTAAAGTATTGAACTTTGATTATGATATATTATTCCCATTGCTCTTTCAAGTATTGATGGTGGCTTTGTATATAAAGTTTCCTCTGCTGAAAATTTGTAGCAAACTTCCATCTCATGTGCTTTGAAGATAAATTGTGTATATT

mRNA sequence

CAAATATTCTCTGTAATTTGTGGACTTCTTTTTCCCAAATCCCATTTCTTCTTCTCCACTTCCCTATTTAAATTCTCCAATCCCCCCTTCACTTTTTCCTTCATACCCATTTCTTCTTCTCTCCCAAATCCCACAAAACATGGCGATTTCTTCACCCATTTTCTTCGCTTTTCTTGCCCTTTTCCTCTGTCTTTCCCCTTTTTCATCCGCTTCCCATTCATCCACCTTCTCCATCATTGATGAAAACGCTAAACACCACTTGGGTATCCCTGAAATTCCCCATTCCGATGCCCATCAACGCACCGACGAGGAAGTTGCCGCTTTGTACGAGTCCTGGTTGGTCCATCATGGAAAAGCCTACAATGCTCTTGGTGAGAAGGAGAGACGGTTTGAGATTTTTAAGGATAATCTCATGTTTATTGATGAACATAACCGGGAATCGCGGACGTATAAAGTTGGATTAACCCGTTTTGCTGATCTTACCAATGAGGAATATCGGGCAAGGTTTTTGGGTGGCCGATTCTCCCGGAAGCCTAGCTTATCCGCCGCCAAGAGCGGAAGGTACGCGGCGGCGCTCGGTGATGATCTTCCGGATGATGTCGATTGGAGAAAAAAGGGCGCTGTTGCTAATGTTAAAGATCAAGGACAGTGTGGGAGCTGTTGGGCTTTCTCAACAGTTGCTGCAGTGGAAGGAATAAACCAAATTGTCACTGGCGAATTGATCTCTCTGTCAGAGCAGGAACTTGTGGATTGTGACAAATCATTTAACATGGGTTGCAATGGTGGTCTTATGGACTATGCTTTCCAGTTCATCATTGACAATGGTGGAATTGACACCGACGAAGATTACCCTTACAAGGGACGAGATGGCGCTTGCGATCCCAATCGGAAAAATGCCAAGGTTGTAACCATAGATGGATATGAAGATGTTCCTGAGAATGATGAGAGCTCCTTGAAAAAGGCTGTAGCAAATCAACCAGTTAGTGTTGCAATTGAAGCTGGTGGCAGAGCCTTCCAACTTTACCAATCGGGTGTCTTCACTGGCCGTTGCGGAACCAATCTAGATCATGGTGTTGTTGCTGTTGGATATGGAACCGACAACGGTACAGATTATTGGATTGTGAGGAATTCATGGGGTAAAGACTGGGGAGAGAATGGTTATATCAGGTTGGAGAGAAATGTGGCCAACAGTACCACAGGCAAGTGTGGTATTGCAGTAGAGCCTTCATACCCCATCAAATCCGGATCAAACCCTCCAAAACCTTCTGCTTCTCCCCCATCTCCTGTCAACCCTCCAACTGAATGCGACGAATACTTCTCGTGCGATGAAGGAAGTACCTGCTGCTGTATCTATCAGTATGGAAGCACCTGCTTTGCTTGGGGATGCTGCCCCCTCGAGTCCGCAACCTGCTGTGATGACCATTACAGTTGCTGCCCACATGAATATCCCGTTTGTGACCTTGAGGCAGGAACTTGCCGAGCGCAAAGACAGCTTGATGGGCGTCAATCTGCTGAAGCGCCTTCCTGCAAATCAGACAAAGCGGATTCAAAAGCTTGGGAAGTTGTTCGTTGGTGCTTGAGAGGTTTGGCAATGGTGGTCGGTGTCGTCAGTGAAATAATCGACCATTTGAACACAGGTCCATAGTATAGAAAAGTTATTAAGAAGTGGAAAAAGGTTGATGTCCCCATTTATTGATCTTTGATATTGTGGGGCTTATTATTTTCCTTTAAAGTATTGAACTTTGATTATGATATATTATTCCCATTGCTCTTTCAAGTATTGATGGTGGCTTTGTATATAAAGTTTCCTCTGCTGAAAATTTGTAGCAAACTTCCATCTCATGTGCTTTGAAGATAAATTGTGTATATT

Coding sequence (CDS)

ATGGCGATTTCTTCACCCATTTTCTTCGCTTTTCTTGCCCTTTTCCTCTGTCTTTCCCCTTTTTCATCCGCTTCCCATTCATCCACCTTCTCCATCATTGATGAAAACGCTAAACACCACTTGGGTATCCCTGAAATTCCCCATTCCGATGCCCATCAACGCACCGACGAGGAAGTTGCCGCTTTGTACGAGTCCTGGTTGGTCCATCATGGAAAAGCCTACAATGCTCTTGGTGAGAAGGAGAGACGGTTTGAGATTTTTAAGGATAATCTCATGTTTATTGATGAACATAACCGGGAATCGCGGACGTATAAAGTTGGATTAACCCGTTTTGCTGATCTTACCAATGAGGAATATCGGGCAAGGTTTTTGGGTGGCCGATTCTCCCGGAAGCCTAGCTTATCCGCCGCCAAGAGCGGAAGGTACGCGGCGGCGCTCGGTGATGATCTTCCGGATGATGTCGATTGGAGAAAAAAGGGCGCTGTTGCTAATGTTAAAGATCAAGGACAGTGTGGGAGCTGTTGGGCTTTCTCAACAGTTGCTGCAGTGGAAGGAATAAACCAAATTGTCACTGGCGAATTGATCTCTCTGTCAGAGCAGGAACTTGTGGATTGTGACAAATCATTTAACATGGGTTGCAATGGTGGTCTTATGGACTATGCTTTCCAGTTCATCATTGACAATGGTGGAATTGACACCGACGAAGATTACCCTTACAAGGGACGAGATGGCGCTTGCGATCCCAATCGGAAAAATGCCAAGGTTGTAACCATAGATGGATATGAAGATGTTCCTGAGAATGATGAGAGCTCCTTGAAAAAGGCTGTAGCAAATCAACCAGTTAGTGTTGCAATTGAAGCTGGTGGCAGAGCCTTCCAACTTTACCAATCGGGTGTCTTCACTGGCCGTTGCGGAACCAATCTAGATCATGGTGTTGTTGCTGTTGGATATGGAACCGACAACGGTACAGATTATTGGATTGTGAGGAATTCATGGGGTAAAGACTGGGGAGAGAATGGTTATATCAGGTTGGAGAGAAATGTGGCCAACAGTACCACAGGCAAGTGTGGTATTGCAGTAGAGCCTTCATACCCCATCAAATCCGGATCAAACCCTCCAAAACCTTCTGCTTCTCCCCCATCTCCTGTCAACCCTCCAACTGAATGCGACGAATACTTCTCGTGCGATGAAGGAAGTACCTGCTGCTGTATCTATCAGTATGGAAGCACCTGCTTTGCTTGGGGATGCTGCCCCCTCGAGTCCGCAACCTGCTGTGATGACCATTACAGTTGCTGCCCACATGAATATCCCGTTTGTGACCTTGAGGCAGGAACTTGCCGAGCGCAAAGACAGCTTGATGGGCGTCAATCTGCTGAAGCGCCTTCCTGCAAATCAGACAAAGCGGATTCAAAAGCTTGGGAAGTTGTTCGTTGGTGCTTGAGAGGTTTGGCAATGGTGGTCGGTGTCGTCAGTGAAATAATCGACCATTTGAACACAGGTCCATAG

Protein sequence

MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVAALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYRARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYKGRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAVEPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWGCCPLESATCCDDHYSCCPHEYPVCDLEAGTCRAQRQLDGRQSAEAPSCKSDKADSKAWEVVRWCLRGLAMVVGVVSEIIDHLNTGP

Homology

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BLAST of Cmc01g0003571 vs. NCBI nr
Match: XP_008454976.1 (PREDICTED: low-temperature-induced cysteine proteinase-like [Cucumis melo])

HSP 1 Score: 931.0 bits (2405), Expect = 4.3e-267
Identity = 448/450 (99.56%), Postives = 449/450 (99.78%), Query Frame = 0


Query: 1   MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA 60
           MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA
Sbjct: 1   MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA 60

Query: 61  ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR 120
           ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR
Sbjct: 61  ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR 120

Query: 121 ARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTV 180
           ARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTV
Sbjct: 121 ARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTV 180

Query: 181 AAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYK 240
           AAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYK
Sbjct: 181 AAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYK 240

Query: 241 GRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF 300
           GRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF
Sbjct: 241 GRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF 300

Query: 301 TGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAV 360
           TGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAV
Sbjct: 301 TGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAV 360

Query: 361 EPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWGCCPLE 420
           EPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWGCCPLE
Sbjct: 361 EPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWGCCPLE 420

Query: 421 SATCCDDHYSCCPHEYPVCDLEAGTCRAQR 451
           SATCCDDHYSCCPHEYPVCDLEAGTCRA +
Sbjct: 421 SATCCDDHYSCCPHEYPVCDLEAGTCRASK 450

[Close]

BLAST of Cmc01g0003571 vs. NCBI nr
Match: KAA0031322.1 (low-temperature-induced cysteine proteinase-like [Cucumis melo var. makuwa] >TYK06773.1 low-temperature-induced cysteine proteinase-like [Cucumis melo var. makuwa])

HSP 1 Score: 919.8 bits (2376), Expect = 9.8e-264
Identity = 447/458 (97.60%), Postives = 448/458 (97.82%), Query Frame = 0


Query: 1   MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA 60
           MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA
Sbjct: 1   MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA 60

Query: 61  ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR 120
           ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR
Sbjct: 61  ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR 120

Query: 121 ARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQ--------CG 180
           ARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQ         G
Sbjct: 121 ARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQKFVMRSIFIG 180

Query: 181 SCWAFSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGID 240
           SCWAFSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGID
Sbjct: 181 SCWAFSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGID 240

Query: 241 TDEDYPYKGRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAF 300
           TDEDYPYKGRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAF
Sbjct: 241 TDEDYPYKGRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAF 300

Query: 301 QLYQSGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANST 360
           QLYQSGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANST
Sbjct: 301 QLYQSGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANST 360

Query: 361 TGKCGIAVEPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCF 420
           TGKCGIAVEPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCF
Sbjct: 361 TGKCGIAVEPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCF 420

Query: 421 AWGCCPLESATCCDDHYSCCPHEYPVCDLEAGTCRAQR 451
           AWGCCPLESATCCDDHYSCCPHEYPVCDLEAGTCRA +
Sbjct: 421 AWGCCPLESATCCDDHYSCCPHEYPVCDLEAGTCRASK 458

[Close]

BLAST of Cmc01g0003571 vs. NCBI nr
Match: XP_004136967.1 (low-temperature-induced cysteine proteinase [Cucumis sativus] >KGN43904.1 hypothetical protein Csa_017340 [Cucumis sativus])

HSP 1 Score: 886.3 bits (2289), Expect = 1.2e-253
Identity = 426/450 (94.67%), Postives = 436/450 (96.89%), Query Frame = 0


Query: 1   MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA 60
           MAI SPIFFAFLALF CLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQR DEEVA
Sbjct: 1   MAI-SPIFFAFLALFFCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRPDEEVA 60

Query: 61  ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR 120
           ALYESWLVHHGKAYNA+GEKERRFEIFKDNL FIDEHNRESRTYKVGLTRFADLTNEEYR
Sbjct: 61  ALYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRESRTYKVGLTRFADLTNEEYR 120

Query: 121 ARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTV 180
           ARFLGGRFSRKP LSAAKSGRYAAALGDDLPDDVDWRKKGAVA VKDQGQCGSCWAFS+V
Sbjct: 121 ARFLGGRFSRKPRLSAAKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFSSV 180

Query: 181 AAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYK 240
           AAVEGINQIVTGELI LSEQELVDCDKSFNMGCNGGLMDYAFQFII NGGIDT+EDYPYK
Sbjct: 181 AAVEGINQIVTGELIPLSEQELVDCDKSFNMGCNGGLMDYAFQFIIGNGGIDTEEDYPYK 240

Query: 241 GRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF 300
           GRD ACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF
Sbjct: 241 GRDAACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF 300

Query: 301 TGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAV 360
           TGRCGT+LDHGVVAVGYGTDNGTDYWIVRNSWGKDWGE+GYIRLERNVAN TTGKCGIAV
Sbjct: 301 TGRCGTDLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGESGYIRLERNVANITTGKCGIAV 360

Query: 361 EPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWGCCPLE 420
           +PSYP KSG+NPPKPSASPPSPV PPTECDEYFSC+EGSTCCCIYQ+GSTCFAWGCCPLE
Sbjct: 361 QPSYPTKSGANPPKPSASPPSPVKPPTECDEYFSCEEGSTCCCIYQFGSTCFAWGCCPLE 420

Query: 421 SATCCDDHYSCCPHEYPVCDLEAGTCRAQR 451
           SATCCDDHYSCCPHEYPVCDLEAGTCR  +
Sbjct: 421 SATCCDDHYSCCPHEYPVCDLEAGTCRVSK 449

[Close]

BLAST of Cmc01g0003571 vs. NCBI nr
Match: XP_038889032.1 (low-temperature-induced cysteine proteinase-like [Benincasa hispida])

HSP 1 Score: 845.5 bits (2183), Expect = 2.3e-241
Identity = 409/452 (90.49%), Postives = 427/452 (94.47%), Query Frame = 0


Query: 1   MAISSPIFF---AFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSD---AHQR 60
           MAISS   F   AFLALFLC SPFSSASHSSTFSIIDENAKHH+GIP+I  SD   + QR
Sbjct: 1   MAISSTFSFLTLAFLALFLCFSPFSSASHSSTFSIIDENAKHHMGIPDIADSDHLRSPQR 60

Query: 61  TDEEVAALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADL 120
           TD+EVAALYESWLVHH KAYNALGEKERRFEIFKDNL FIDEHNRESRTYKVGLTRFADL
Sbjct: 61  TDDEVAALYESWLVHHRKAYNALGEKERRFEIFKDNLRFIDEHNRESRTYKVGLTRFADL 120

Query: 121 TNEEYRARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSC 180
           TNEEYRARFLGGRF  KP LSAAKSGRYAAALGDDLPD VDWRKKGAVA VKDQGQCGSC
Sbjct: 121 TNEEYRARFLGGRFPGKPRLSAAKSGRYAAALGDDLPDHVDWRKKGAVAAVKDQGQCGSC 180

Query: 181 WAFSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTD 240
           WAFSTVAAVEGINQIVTGELISLSEQELVDCDKS+NMGCNGGLMDYAFQFIIDNGGIDT+
Sbjct: 181 WAFSTVAAVEGINQIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFIIDNGGIDTE 240

Query: 241 EDYPYKGRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQL 300
           EDYPYKG D ACDPNRKNAKVVTIDG+EDVPENDESSLKKAVANQPVSVAIEAGGRAFQL
Sbjct: 241 EDYPYKGHDAACDPNRKNAKVVTIDGFEDVPENDESSLKKAVANQPVSVAIEAGGRAFQL 300

Query: 301 YQSGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTG 360
           YQSGVFTGRCGTNLDHGVVAVGYGT+NGTDYWIVRNSWGK+WGE+GYIRLERNVAN TTG
Sbjct: 301 YQSGVFTGRCGTNLDHGVVAVGYGTENGTDYWIVRNSWGKNWGESGYIRLERNVANITTG 360

Query: 361 KCGIAVEPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAW 420
           KCGIAV+PSYPIK+GSNPPKP+ASPPSPV PPTECD+Y+SC+EG+TCCCIYQYGSTCF W
Sbjct: 361 KCGIAVQPSYPIKTGSNPPKPAASPPSPVKPPTECDDYYSCEEGTTCCCIYQYGSTCFGW 420

Query: 421 GCCPLESATCCDDHYSCCPHEYPVCDLEAGTC 447
           GCCPLESATCCDDHYSCCPHEYP+CDLEAGTC
Sbjct: 421 GCCPLESATCCDDHYSCCPHEYPICDLEAGTC 452

[Close]

BLAST of Cmc01g0003571 vs. NCBI nr
Match: XP_022147795.1 (low-temperature-induced cysteine proteinase-like [Momordica charantia])

HSP 1 Score: 785.0 bits (2026), Expect = 3.8e-223
Identity = 378/456 (82.89%), Postives = 408/456 (89.47%), Query Frame = 0


Query: 1   MAISSPIFF---AFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQ---R 60
           MAI S   F   A LALFLC   FSSAS SS+FSIIDENAKHHLG P+I  SDA     R
Sbjct: 1   MAIFSSFSFPILASLALFLCFLSFSSASDSSSFSIIDENAKHHLGFPDIAGSDAGTPPLR 60

Query: 61  TDEEVAALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADL 120
           T E+VAALY+SWLV HGKAYNALGE+ERRFEIFKDNL FIDEHNRE R+Y +GLTRFADL
Sbjct: 61  TQEQVAALYKSWLVKHGKAYNALGERERRFEIFKDNLRFIDEHNREPRSYTLGLTRFADL 120

Query: 121 TNEEYRARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSC 180
           TNEEYRARFLGGRFS KP  SAAK+GRYA++LG DLP+ VDWR+KGAV  VKDQGQCGSC
Sbjct: 121 TNEEYRARFLGGRFSPKPRPSAAKNGRYASSLGGDLPEHVDWREKGAVTAVKDQGQCGSC 180

Query: 181 WAFSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTD 240
           WAFST+AAVEGINQIVTGELISLSEQELVDCDKS+NMGCNGGLMDYAFQFI+DNGGIDT+
Sbjct: 181 WAFSTIAAVEGINQIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFIVDNGGIDTE 240

Query: 241 EDYPYKGRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQL 300
           EDYPYKGRDG CDPNR+NA VVTIDGYEDVPENDE +LKKAVA+QPVSVAIEAGGRAFQL
Sbjct: 241 EDYPYKGRDGVCDPNRRNANVVTIDGYEDVPENDEGALKKAVASQPVSVAIEAGGRAFQL 300

Query: 301 YQSGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTG 360
           YQSGVFTGRCGT+LDHGVVAVGYGT+NG DYWIVRNSWGK+WGENGYIRLERNVAN TTG
Sbjct: 301 YQSGVFTGRCGTDLDHGVVAVGYGTENGLDYWIVRNSWGKNWGENGYIRLERNVANITTG 360

Query: 361 KCGIAVEPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAW 420
           KCGIA+EPSYP+K+G NPPKP+ SPPSPV PPTECD+Y+SC EG+TCCCIYQYGSTCF W
Sbjct: 361 KCGIAIEPSYPVKTGKNPPKPAPSPPSPVKPPTECDDYYSCPEGTTCCCIYQYGSTCFGW 420

Query: 421 GCCPLESATCCDDHYSCCPHEYPVCDLEAGTCRAQR 451
           GCCPLESATCCDD YSCCP EYPVCDL  GTCR  +
Sbjct: 421 GCCPLESATCCDDQYSCCPREYPVCDLAEGTCRMSK 456

[Close]

BLAST of Cmc01g0003571 vs. ExPASy Swiss-Prot
Match: P43297 (Cysteine proteinase RD21A OS=Arabidopsis thaliana OX=3702 GN=RD21A PE=1 SV=1)

HSP 1 Score: 623.6 bits (1607), Expect = 1.9e-177
Identity = 296/435 (68.05%), Postives = 352/435 (80.92%), Query Frame = 0


Query: 14  LFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVAALYESWLVHHGKA 73
           LFL +   SSA      SII  + KH +       S    R++ EV ++YE+WLV HGKA
Sbjct: 11  LFLAMVAVSSA---VDMSIISYDEKHGV-------STTGGRSEAEVMSIYEAWLVKHGKA 70

Query: 74  --YNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYRARFLGGRFSRK 133
              N+L EK+RRFEIFKDNL F+DEHN ++ +Y++GLTRFADLTN+EYR+++LG +  +K
Sbjct: 71  QSQNSLVEKDRRFEIFKDNLRFVDEHNEKNLSYRLGLTRFADLTNDEYRSKYLGAKMEKK 130

Query: 134 PSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTVAAVEGINQIVT 193
                  S RY A +GD+LP+ +DWRKKGAVA VKDQG CGSCWAFST+ AVEGINQIVT
Sbjct: 131 G--ERRTSLRYEARVGDELPESIDWRKKGAVAEVKDQGGCGSCWAFSTIGAVEGINQIVT 190

Query: 194 GELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYKGRDGACDPNRK 253
           G+LI+LSEQELVDCD S+N GCNGGLMDYAF+FII NGGIDTD+DYPYKG DG CD  RK
Sbjct: 191 GDLITLSEQELVDCDTSYNEGCNGGLMDYAFEFIIKNGGIDTDKDYPYKGVDGTCDQIRK 250

Query: 254 NAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTGRCGTNLDHG 313
           NAKVVTID YEDVP   E SLKKAVA+QP+S+AIEAGGRAFQLY SG+F G CGT LDHG
Sbjct: 251 NAKVVTIDSYEDVPTYSEESLKKAVAHQPISIAIEAGGRAFQLYDSGIFDGSCGTQLDHG 310

Query: 314 VVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAVEPSYPIKSGSN 373
           VVAVGYGT+NG DYWIVRNSWGK WGE+GY+R+ RN+A S++GKCGIA+EPSYPIK+G N
Sbjct: 311 VVAVGYGTENGKDYWIVRNSWGKSWGESGYLRMARNIA-SSSGKCGIAIEPSYPIKNGEN 370

Query: 374 PPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWGCCPLESATCCDDHYSC 433
           PP P  SPPSP+ PPT+CD Y++C E +TCCC+++YG  CFAWGCCPLE+ATCCDD+YSC
Sbjct: 371 PPNPGPSPPSPIKPPTQCDSYYTCPESNTCCCLFEYGKYCFAWGCCPLEAATCCDDNYSC 430

Query: 434 CPHEYPVCDLEAGTC 447
           CPHEYPVCDL+ GTC
Sbjct: 431 CPHEYPVCDLDQGTC 432

[Close]

BLAST of Cmc01g0003571 vs. ExPASy Swiss-Prot
Match: Q9FMH8 (Probable cysteine protease RD21B OS=Arabidopsis thaliana OX=3702 GN=RD21B PE=1 SV=1)

HSP 1 Score: 612.1 bits (1577), Expect = 5.7e-174
Identity = 297/443 (67.04%), Postives = 345/443 (77.88%), Query Frame = 0


Query: 9   FAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVAALYESWLV 68
           F  L+  + L      S++   SII  +  HH+       +    R+D EV  +YE+W+V
Sbjct: 3   FLKLSPMILLLAMIGVSYAMDMSIISYDENHHI-------TTETSRSDSEVERIYEAWMV 62

Query: 69  HHGKA---YNALG-EKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYRARFL 128
            HGK     N LG EK++RFEIFKDNL FIDEHN ++ +YK+GLTRFADLTNEEYR+ +L
Sbjct: 63  EHGKKKMNQNGLGAEKDQRFEIFKDNLRFIDEHNTKNLSYKLGLTRFADLTNEEYRSMYL 122

Query: 129 GGRFSRKPSLSAAK-SGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTVAAV 188
           G     KP+    K S RY A +GD LPD VDWRK+GAVA+VKDQG CGSCWAFST+ AV
Sbjct: 123 GA----KPTKRVLKTSDRYQARVGDALPDSVDWRKEGAVADVKDQGSCGSCWAFSTIGAV 182

Query: 189 EGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYKGRD 248
           EGIN+IVTG+LISLSEQELVDCD S+N GCNGGLMDYAF+FII NGGIDT+ DYPYK  D
Sbjct: 183 EGINKIVTGDLISLSEQELVDCDTSYNQGCNGGLMDYAFEFIIKNGGIDTEADYPYKAAD 242

Query: 249 GACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTGR 308
           G CD NRKNAKVVTID YEDVPEN E+SLKKA+A+QP+SVAIEAGGRAFQLY SGVF G 
Sbjct: 243 GRCDQNRKNAKVVTIDSYEDVPENSEASLKKALAHQPISVAIEAGGRAFQLYSSGVFDGL 302

Query: 309 CGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAVEPS 368
           CGT LDHGVVAVGYGT+NG DYWIVRNSWG  WGE+GYI++ RN+  + TGKCGIA+E S
Sbjct: 303 CGTELDHGVVAVGYGTENGKDYWIVRNSWGNRWGESGYIKMARNI-EAPTGKCGIAMEAS 362

Query: 369 YPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWGCCPLESAT 428
           YPIK G NPP P  SPPSP+ PPT CD+YFSC E +TCCC+Y+YG  CF WGCCPLE+AT
Sbjct: 363 YPIKKGQNPPNPGPSPPSPIKPPTTCDKYFSCPESNTCCCLYKYGKYCFGWGCCPLEAAT 422

Query: 429 CCDDHYSCCPHEYPVCDLEAGTC 447
           CCDD+ SCCPHEYPVCD+  GTC
Sbjct: 423 CCDDNSSCCPHEYPVCDVNRGTC 433

[Close]

BLAST of Cmc01g0003571 vs. ExPASy Swiss-Prot
Match: P25776 (Oryzain alpha chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0650000 PE=1 SV=2)

HSP 1 Score: 601.3 bits (1549), Expect = 1.0e-170
Identity = 276/398 (69.35%), Postives = 325/398 (81.66%), Query Frame = 0


Query: 53  QRTDEEVAALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRES----RTYKVGL 112
           +R++EE   LY  W   HGK+YNA+GE+ERR+  F+DNL +IDEHN  +     ++++GL
Sbjct: 30  ERSEEEARRLYAEWKAEHGKSYNAVGEEERRYAAFRDNLRYIDEHNAAADAGVHSFRLGL 89

Query: 113 TRFADLTNEEYRARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQ 172
            RFADLTNEEYR  +LG R   KP      S RY AA  + LP+ VDWR KGAVA +KDQ
Sbjct: 90  NRFADLTNEEYRDTYLGLR--NKPRRERKVSDRYLAADNEALPESVDWRTKGAVAEIKDQ 149

Query: 173 GQCGSCWAFSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDN 232
           G CGSCWAFS +AAVEGINQIVTG+LISLSEQELVDCD S+N GCNGGLMDYAF FII+N
Sbjct: 150 GGCGSCWAFSAIAAVEGINQIVTGDLISLSEQELVDCDTSYNEGCNGGLMDYAFDFIINN 209

Query: 233 GGIDTDEDYPYKGRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAG 292
           GGIDT++DYPYKG+D  CD NRKNAKVVTID YEDV  N E+SL+KAVANQPVSVAIEAG
Sbjct: 210 GGIDTEDDYPYKGKDERCDVNRKNAKVVTIDSYEDVTPNSETSLQKAVANQPVSVAIEAG 269

Query: 293 GRAFQLYQSGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNV 352
           GRAFQLY SG+FTG+CGT LDHGV AVGYGT+NG DYWIVRNSWGK WGE+GY+R+ERN+
Sbjct: 270 GRAFQLYSSGIFTGKCGTALDHGVAAVGYGTENGKDYWIVRNSWGKSWGESGYVRMERNI 329

Query: 353 ANSTTGKCGIAVEPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYG 412
             +++GKCGIAVEPSYP+K G NPP P  +PPSP  PPT CD Y++C + +TCCCIY+YG
Sbjct: 330 -KASSGKCGIAVEPSYPLKKGENPPNPGPTPPSPTPPPTVCDNYYTCPDSTTCCCIYEYG 389

Query: 413 STCFAWGCCPLESATCCDDHYSCCPHEYPVCDLEAGTC 447
             C+AWGCCPLE ATCCDDHYSCCPHEYP+C+++ GTC
Sbjct: 390 KYCYAWGCCPLEGATCCDDHYSCCPHEYPICNVQQGTC 424

[Close]

BLAST of Cmc01g0003571 vs. ExPASy Swiss-Prot
Match: Q9LT78 (Probable cysteine protease RD21C OS=Arabidopsis thaliana OX=3702 GN=RD21C PE=1 SV=1)

HSP 1 Score: 544.7 bits (1402), Expect = 1.1e-153
Identity = 266/397 (67.00%), Postives = 311/397 (78.34%), Query Frame = 0


Query: 54  RTDEEVAALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNR-ESRTYKVGLTRFA 113
           R + E   +YE WLV + K YN LGEKERRFEIFKDNL F++EH+   +RTY+VGLTRFA
Sbjct: 34  RNEAEARRMYERWLVENRKNYNGLGEKERRFEIFKDNLKFVEEHSSIPNRTYEVGLTRFA 93

Query: 114 DLTNEEYRARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCG 173
           DLTN+E+RA +L  +  R  +    K  +Y   +GD LPD +DWR KGAV  VKDQG CG
Sbjct: 94  DLTNDEFRAIYLRSKMER--TRVPVKGEKYLYKVGDSLPDAIDWRAKGAVNPVKDQGSCG 153

Query: 174 SCWAFSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGID 233
           SCWAFS + AVEGINQI TGELISLSEQELVDCD S+N GC GGLMDYAF+FII+NGGID
Sbjct: 154 SCWAFSAIGAVEGINQIKTGELISLSEQELVDCDTSYNDGCGGGLMDYAFKFIIENGGID 213

Query: 234 TDEDYPYKGRD-GACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRA 293
           T+EDYPY   D   C+ ++KN +VVTIDGYEDVP+NDE SLKKA+ANQP+SVAIEAGGRA
Sbjct: 214 TEEDYPYIATDVNVCNSDKKNTRVVTIDGYEDVPQNDEKSLKKALANQPISVAIEAGGRA 273

Query: 294 FQLYQSGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANS 353
           FQLY SGVFTG CGT+LDHGVVAVGYG++ G DYWIVRNSWG +WGE+GY +LERN+  S
Sbjct: 274 FQLYTSGVFTGTCGTSLDHGVVAVGYGSEGGQDYWIVRNSWGSNWGESGYFKLERNIKES 333

Query: 354 TTGKCGIAVEPSYPIK-SGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGST 413
            +GKCG+A+  SYP K SGSNPPKP A  PSPV     CD+  +C   STCCC+Y+Y   
Sbjct: 334 -SGKCGVAMMASYPTKSSGSNPPKPPA--PSPV----VCDKSNTCPAKSTCCCLYEYNGK 393

Query: 414 CFAWGCCPLESATCCDDHYSCCPHEYPVCDLEAGTCR 448
           C++WGCCP ESATCCDD  SCCP  YPVCDL+A TCR
Sbjct: 394 CYSWGCCPYESATCCDDGSSCCPQSYPVCDLKANTCR 421

[Close]

BLAST of Cmc01g0003571 vs. ExPASy Swiss-Prot
Match: P25777 (Oryzain beta chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0670200 PE=1 SV=2)

HSP 1 Score: 534.3 bits (1375), Expect = 1.5e-150
Identity = 276/454 (60.79%), Postives = 331/454 (72.91%), Query Frame = 0


Query: 10  AFLALFLCLSPFSSASHSSTFSIIDENAKHHL-GIPEIPHSDAHQRTDEEVAALYESWLV 69
           A  A FL L    +A+ +   SII  NA+H   G+ E P       T+ E  A Y+ WL 
Sbjct: 5   AAAAAFLLLLIVGAATAAPDMSIISYNAEHGARGLEEGP-------TEAEARAAYDLWLA 64

Query: 70  HH-GKAYNAL-GEKERRFEIFKDNLMFIDEHNR---ESRTYKVGLTRFADLTNEEYRARF 129
            + G + NAL GE ERRF +F DNL F+D HN    E   +++G+ RFADLTNEE+RA F
Sbjct: 65  ENGGGSPNALGGEHERRFLVFWDNLKFVDAHNARADERGGFRLGMNRFADLTNEEFRATF 124

Query: 130 LGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTVAAV 189
           LG + + +   S A   RY     ++LP+ VDWR+KGAVA VK+QGQCGSCWAFS V+ V
Sbjct: 125 LGAKVAER---SRAAGERYRHDGVEELPESVDWREKGAVAPVKNQGQCGSCWAFSAVSTV 184

Query: 190 EGINQIVTGELISLSEQELVDCD-KSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYKGR 249
           E INQ+VTGE+I+LSEQELV+C     N GCNGGLMD AF FII NGGIDT++DYPYK  
Sbjct: 185 ESINQLVTGEMITLSEQELVECSTNGQNSGCNGGLMDDAFDFIIKNGGIDTEDDYPYKAV 244

Query: 250 DGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTG 309
           DG CD NR+NAKVV+IDG+EDVP+NDE SL+KAVA+QPVSVAIEAGGR FQLY SGVF+G
Sbjct: 245 DGKCDINRENAKVVSIDGFEDVPQNDEKSLQKAVAHQPVSVAIEAGGREFQLYHSGVFSG 304

Query: 310 RCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAVEP 369
           RCGT+LDHGVVAVGYGTDNG DYWIVRNSWG  WGE+GY+R+ERN+ N TTGKCGIA+  
Sbjct: 305 RCGTSLDHGVVAVGYGTDNGKDYWIVRNSWGPKWGESGYVRMERNI-NVTTGKCGIAMMA 364

Query: 370 SYPIKSGSNPPKPSASPPSPVNPPTE------CDEYFSCDEGSTCCCIYQYGSTCFAWGC 429
           SYP KSG+NPPKPS +PP+P  PP        CD+ FSC  GSTCCC + + + C  WGC
Sbjct: 365 SYPTKSGANPPKPSPTPPTPPTPPPPSAPDHVCDDNFSCPAGSTCCCAFGFRNLCLVWGC 424

Query: 430 CPLESATCCDDHYSCCPHEYPVCDLEAGTCRAQR 451
           CP+E ATCC DH SCCP +YPVC+  AGTC A +
Sbjct: 425 CPVEGATCCKDHASCCPPDYPVCNTRAGTCSASK 447

[Close]

BLAST of Cmc01g0003571 vs. ExPASy TrEMBL
Match: A0A1S3BZW8 (low-temperature-induced cysteine proteinase-like OS=Cucumis melo OX=3656 GN=LOC103495259 PE=3 SV=1)

HSP 1 Score: 931.0 bits (2405), Expect = 2.1e-267
Identity = 448/450 (99.56%), Postives = 449/450 (99.78%), Query Frame = 0


Query: 1   MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA 60
           MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA
Sbjct: 1   MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA 60

Query: 61  ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR 120
           ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR
Sbjct: 61  ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR 120

Query: 121 ARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTV 180
           ARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTV
Sbjct: 121 ARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTV 180

Query: 181 AAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYK 240
           AAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYK
Sbjct: 181 AAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYK 240

Query: 241 GRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF 300
           GRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF
Sbjct: 241 GRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF 300

Query: 301 TGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAV 360
           TGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAV
Sbjct: 301 TGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAV 360

Query: 361 EPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWGCCPLE 420
           EPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWGCCPLE
Sbjct: 361 EPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWGCCPLE 420

Query: 421 SATCCDDHYSCCPHEYPVCDLEAGTCRAQR 451
           SATCCDDHYSCCPHEYPVCDLEAGTCRA +
Sbjct: 421 SATCCDDHYSCCPHEYPVCDLEAGTCRASK 450

[Close]

BLAST of Cmc01g0003571 vs. ExPASy TrEMBL
Match: A0A5A7SJU5 (Low-temperature-induced cysteine proteinase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00750 PE=3 SV=1)

HSP 1 Score: 919.8 bits (2376), Expect = 4.7e-264
Identity = 447/458 (97.60%), Postives = 448/458 (97.82%), Query Frame = 0


Query: 1   MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA 60
           MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA
Sbjct: 1   MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA 60

Query: 61  ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR 120
           ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR
Sbjct: 61  ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR 120

Query: 121 ARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQ--------CG 180
           ARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQ         G
Sbjct: 121 ARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQKFVMRSIFIG 180

Query: 181 SCWAFSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGID 240
           SCWAFSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGID
Sbjct: 181 SCWAFSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGID 240

Query: 241 TDEDYPYKGRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAF 300
           TDEDYPYKGRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAF
Sbjct: 241 TDEDYPYKGRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAF 300

Query: 301 QLYQSGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANST 360
           QLYQSGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANST
Sbjct: 301 QLYQSGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANST 360

Query: 361 TGKCGIAVEPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCF 420
           TGKCGIAVEPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCF
Sbjct: 361 TGKCGIAVEPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCF 420

Query: 421 AWGCCPLESATCCDDHYSCCPHEYPVCDLEAGTCRAQR 451
           AWGCCPLESATCCDDHYSCCPHEYPVCDLEAGTCRA +
Sbjct: 421 AWGCCPLESATCCDDHYSCCPHEYPVCDLEAGTCRASK 458

[Close]

BLAST of Cmc01g0003571 vs. ExPASy TrEMBL
Match: A0A0A0K4N3 (Cysteine protease OS=Cucumis sativus OX=3659 GN=Csa_7G073400 PE=3 SV=1)

HSP 1 Score: 886.3 bits (2289), Expect = 5.8e-254
Identity = 426/450 (94.67%), Postives = 436/450 (96.89%), Query Frame = 0


Query: 1   MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA 60
           MAI SPIFFAFLALF CLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQR DEEVA
Sbjct: 1   MAI-SPIFFAFLALFFCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRPDEEVA 60

Query: 61  ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR 120
           ALYESWLVHHGKAYNA+GEKERRFEIFKDNL FIDEHNRESRTYKVGLTRFADLTNEEYR
Sbjct: 61  ALYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRESRTYKVGLTRFADLTNEEYR 120

Query: 121 ARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTV 180
           ARFLGGRFSRKP LSAAKSGRYAAALGDDLPDDVDWRKKGAVA VKDQGQCGSCWAFS+V
Sbjct: 121 ARFLGGRFSRKPRLSAAKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFSSV 180

Query: 181 AAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYK 240
           AAVEGINQIVTGELI LSEQELVDCDKSFNMGCNGGLMDYAFQFII NGGIDT+EDYPYK
Sbjct: 181 AAVEGINQIVTGELIPLSEQELVDCDKSFNMGCNGGLMDYAFQFIIGNGGIDTEEDYPYK 240

Query: 241 GRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF 300
           GRD ACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF
Sbjct: 241 GRDAACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF 300

Query: 301 TGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAV 360
           TGRCGT+LDHGVVAVGYGTDNGTDYWIVRNSWGKDWGE+GYIRLERNVAN TTGKCGIAV
Sbjct: 301 TGRCGTDLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGESGYIRLERNVANITTGKCGIAV 360

Query: 361 EPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWGCCPLE 420
           +PSYP KSG+NPPKPSASPPSPV PPTECDEYFSC+EGSTCCCIYQ+GSTCFAWGCCPLE
Sbjct: 361 QPSYPTKSGANPPKPSASPPSPVKPPTECDEYFSCEEGSTCCCIYQFGSTCFAWGCCPLE 420

Query: 421 SATCCDDHYSCCPHEYPVCDLEAGTCRAQR 451
           SATCCDDHYSCCPHEYPVCDLEAGTCR  +
Sbjct: 421 SATCCDDHYSCCPHEYPVCDLEAGTCRVSK 449

[Close]

BLAST of Cmc01g0003571 vs. ExPASy TrEMBL
Match: A0A6J1D149 (low-temperature-induced cysteine proteinase-like OS=Momordica charantia OX=3673 GN=LOC111016644 PE=3 SV=1)

HSP 1 Score: 785.0 bits (2026), Expect = 1.8e-223
Identity = 378/456 (82.89%), Postives = 408/456 (89.47%), Query Frame = 0


Query: 1   MAISSPIFF---AFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQ---R 60
           MAI S   F   A LALFLC   FSSAS SS+FSIIDENAKHHLG P+I  SDA     R
Sbjct: 1   MAIFSSFSFPILASLALFLCFLSFSSASDSSSFSIIDENAKHHLGFPDIAGSDAGTPPLR 60

Query: 61  TDEEVAALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADL 120
           T E+VAALY+SWLV HGKAYNALGE+ERRFEIFKDNL FIDEHNRE R+Y +GLTRFADL
Sbjct: 61  TQEQVAALYKSWLVKHGKAYNALGERERRFEIFKDNLRFIDEHNREPRSYTLGLTRFADL 120

Query: 121 TNEEYRARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSC 180
           TNEEYRARFLGGRFS KP  SAAK+GRYA++LG DLP+ VDWR+KGAV  VKDQGQCGSC
Sbjct: 121 TNEEYRARFLGGRFSPKPRPSAAKNGRYASSLGGDLPEHVDWREKGAVTAVKDQGQCGSC 180

Query: 181 WAFSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTD 240
           WAFST+AAVEGINQIVTGELISLSEQELVDCDKS+NMGCNGGLMDYAFQFI+DNGGIDT+
Sbjct: 181 WAFSTIAAVEGINQIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFIVDNGGIDTE 240

Query: 241 EDYPYKGRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQL 300
           EDYPYKGRDG CDPNR+NA VVTIDGYEDVPENDE +LKKAVA+QPVSVAIEAGGRAFQL
Sbjct: 241 EDYPYKGRDGVCDPNRRNANVVTIDGYEDVPENDEGALKKAVASQPVSVAIEAGGRAFQL 300

Query: 301 YQSGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTG 360
           YQSGVFTGRCGT+LDHGVVAVGYGT+NG DYWIVRNSWGK+WGENGYIRLERNVAN TTG
Sbjct: 301 YQSGVFTGRCGTDLDHGVVAVGYGTENGLDYWIVRNSWGKNWGENGYIRLERNVANITTG 360

Query: 361 KCGIAVEPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAW 420
           KCGIA+EPSYP+K+G NPPKP+ SPPSPV PPTECD+Y+SC EG+TCCCIYQYGSTCF W
Sbjct: 361 KCGIAIEPSYPVKTGKNPPKPAPSPPSPVKPPTECDDYYSCPEGTTCCCIYQYGSTCFGW 420

Query: 421 GCCPLESATCCDDHYSCCPHEYPVCDLEAGTCRAQR 451
           GCCPLESATCCDD YSCCP EYPVCDL  GTCR  +
Sbjct: 421 GCCPLESATCCDDQYSCCPREYPVCDLAEGTCRMSK 456

[Close]

BLAST of Cmc01g0003571 vs. ExPASy TrEMBL
Match: A0A6J1EI11 (low-temperature-induced cysteine proteinase-like OS=Cucurbita moschata OX=3662 GN=LOC111434545 PE=3 SV=1)

HSP 1 Score: 773.1 bits (1995), Expect = 7.2e-220
Identity = 374/449 (83.30%), Postives = 400/449 (89.09%), Query Frame = 0


Query: 1   MAISSPIFFA---FLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDE 60
           MAIS+   F+   FL LFLC S  SSAS SS+FSIIDENA+HHL I       + QR+DE
Sbjct: 1   MAISTTFCFSIVVFLPLFLCFSSSSSASDSSSFSIIDENARHHLDI----QIGSSQRSDE 60

Query: 61  EVAALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNE 120
           EVAALYESWLVH+GKAYNALGEKERRFEIFKDNL FIDEHNRESR+YKVGL RFADL+NE
Sbjct: 61  EVAALYESWLVHNGKAYNALGEKERRFEIFKDNLRFIDEHNRESRSYKVGLNRFADLSNE 120

Query: 121 EYRARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAF 180
           EYRA FL GR SRK  LSA KS RYA ALGDDLPD VDWRKKGAV +VKDQGQCGSCWAF
Sbjct: 121 EYRAMFLSGRLSRKNRLSATKSRRYAIALGDDLPDHVDWRKKGAVTDVKDQGQCGSCWAF 180

Query: 181 STVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDY 240
           STVAAVEGINQI TGELISLSEQELVDCD+S+NMGCNGGLMDYAFQFIIDNGGIDT+EDY
Sbjct: 181 STVAAVEGINQITTGELISLSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGIDTEEDY 240

Query: 241 PYKGRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQS 300
           PYKGRDG CDP RKNAK VTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQS
Sbjct: 241 PYKGRDGTCDPIRKNAKAVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQS 300

Query: 301 GVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCG 360
           GVF G CGT LDHGVVAVGYGT++G DYWIVRNSWGK+WGENGYI+LERNV N TTGKCG
Sbjct: 301 GVFNGLCGTELDHGVVAVGYGTEDGKDYWIVRNSWGKNWGENGYIKLERNVGNITTGKCG 360

Query: 361 IAVEPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWGCC 420
           IA+E SYP K+G NPPKP+ SPPSPV+PP +CDEYFSC EG+TCCCIY+YGSTCF WGCC
Sbjct: 361 IAIEASYPTKTGKNPPKPAPSPPSPVSPPNQCDEYFSCQEGTTCCCIYKYGSTCFGWGCC 420

Query: 421 PLESATCCDDHYSCCPHEYPVCDLEAGTC 447
           PLESATCCDDHYSCCPHEYP+CDL+AGTC
Sbjct: 421 PLESATCCDDHYSCCPHEYPICDLDAGTC 445

[Close]

BLAST of Cmc01g0003571 vs. TAIR 10
Match: AT1G47128.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 623.6 bits (1607), Expect = 1.4e-178
Identity = 296/435 (68.05%), Postives = 352/435 (80.92%), Query Frame = 0


Query: 14  LFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVAALYESWLVHHGKA 73
           LFL +   SSA      SII  + KH +       S    R++ EV ++YE+WLV HGKA
Sbjct: 11  LFLAMVAVSSA---VDMSIISYDEKHGV-------STTGGRSEAEVMSIYEAWLVKHGKA 70

Query: 74  --YNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYRARFLGGRFSRK 133
              N+L EK+RRFEIFKDNL F+DEHN ++ +Y++GLTRFADLTN+EYR+++LG +  +K
Sbjct: 71  QSQNSLVEKDRRFEIFKDNLRFVDEHNEKNLSYRLGLTRFADLTNDEYRSKYLGAKMEKK 130

Query: 134 PSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTVAAVEGINQIVT 193
                  S RY A +GD+LP+ +DWRKKGAVA VKDQG CGSCWAFST+ AVEGINQIVT
Sbjct: 131 G--ERRTSLRYEARVGDELPESIDWRKKGAVAEVKDQGGCGSCWAFSTIGAVEGINQIVT 190

Query: 194 GELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYKGRDGACDPNRK 253
           G+LI+LSEQELVDCD S+N GCNGGLMDYAF+FII NGGIDTD+DYPYKG DG CD  RK
Sbjct: 191 GDLITLSEQELVDCDTSYNEGCNGGLMDYAFEFIIKNGGIDTDKDYPYKGVDGTCDQIRK 250

Query: 254 NAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTGRCGTNLDHG 313
           NAKVVTID YEDVP   E SLKKAVA+QP+S+AIEAGGRAFQLY SG+F G CGT LDHG
Sbjct: 251 NAKVVTIDSYEDVPTYSEESLKKAVAHQPISIAIEAGGRAFQLYDSGIFDGSCGTQLDHG 310

Query: 314 VVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAVEPSYPIKSGSN 373
           VVAVGYGT+NG DYWIVRNSWGK WGE+GY+R+ RN+A S++GKCGIA+EPSYPIK+G N
Sbjct: 311 VVAVGYGTENGKDYWIVRNSWGKSWGESGYLRMARNIA-SSSGKCGIAIEPSYPIKNGEN 370

Query: 374 PPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWGCCPLESATCCDDHYSC 433
           PP P  SPPSP+ PPT+CD Y++C E +TCCC+++YG  CFAWGCCPLE+ATCCDD+YSC
Sbjct: 371 PPNPGPSPPSPIKPPTQCDSYYTCPESNTCCCLFEYGKYCFAWGCCPLEAATCCDDNYSC 430

Query: 434 CPHEYPVCDLEAGTC 447
           CPHEYPVCDL+ GTC
Sbjct: 431 CPHEYPVCDLDQGTC 432

[Close]

BLAST of Cmc01g0003571 vs. TAIR 10
Match: AT5G43060.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 612.1 bits (1577), Expect = 4.1e-175
Identity = 297/443 (67.04%), Postives = 345/443 (77.88%), Query Frame = 0


Query: 9   FAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVAALYESWLV 68
           F  L+  + L      S++   SII  +  HH+       +    R+D EV  +YE+W+V
Sbjct: 3   FLKLSPMILLLAMIGVSYAMDMSIISYDENHHI-------TTETSRSDSEVERIYEAWMV 62

Query: 69  HHGKA---YNALG-EKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYRARFL 128
            HGK     N LG EK++RFEIFKDNL FIDEHN ++ +YK+GLTRFADLTNEEYR+ +L
Sbjct: 63  EHGKKKMNQNGLGAEKDQRFEIFKDNLRFIDEHNTKNLSYKLGLTRFADLTNEEYRSMYL 122

Query: 129 GGRFSRKPSLSAAK-SGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTVAAV 188
           G     KP+    K S RY A +GD LPD VDWRK+GAVA+VKDQG CGSCWAFST+ AV
Sbjct: 123 GA----KPTKRVLKTSDRYQARVGDALPDSVDWRKEGAVADVKDQGSCGSCWAFSTIGAV 182

Query: 189 EGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYKGRD 248
           EGIN+IVTG+LISLSEQELVDCD S+N GCNGGLMDYAF+FII NGGIDT+ DYPYK  D
Sbjct: 183 EGINKIVTGDLISLSEQELVDCDTSYNQGCNGGLMDYAFEFIIKNGGIDTEADYPYKAAD 242

Query: 249 GACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTGR 308
           G CD NRKNAKVVTID YEDVPEN E+SLKKA+A+QP+SVAIEAGGRAFQLY SGVF G 
Sbjct: 243 GRCDQNRKNAKVVTIDSYEDVPENSEASLKKALAHQPISVAIEAGGRAFQLYSSGVFDGL 302

Query: 309 CGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAVEPS 368
           CGT LDHGVVAVGYGT+NG DYWIVRNSWG  WGE+GYI++ RN+  + TGKCGIA+E S
Sbjct: 303 CGTELDHGVVAVGYGTENGKDYWIVRNSWGNRWGESGYIKMARNI-EAPTGKCGIAMEAS 362

Query: 369 YPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWGCCPLESAT 428
           YPIK G NPP P  SPPSP+ PPT CD+YFSC E +TCCC+Y+YG  CF WGCCPLE+AT
Sbjct: 363 YPIKKGQNPPNPGPSPPSPIKPPTTCDKYFSCPESNTCCCLYKYGKYCFGWGCCPLEAAT 422

Query: 429 CCDDHYSCCPHEYPVCDLEAGTC 447
           CCDD+ SCCPHEYPVCD+  GTC
Sbjct: 423 CCDDNSSCCPHEYPVCDVNRGTC 433

[Close]

BLAST of Cmc01g0003571 vs. TAIR 10
Match: AT3G19390.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 544.7 bits (1402), Expect = 8.0e-155
Identity = 266/397 (67.00%), Postives = 311/397 (78.34%), Query Frame = 0


Query: 54  RTDEEVAALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNR-ESRTYKVGLTRFA 113
           R + E   +YE WLV + K YN LGEKERRFEIFKDNL F++EH+   +RTY+VGLTRFA
Sbjct: 34  RNEAEARRMYERWLVENRKNYNGLGEKERRFEIFKDNLKFVEEHSSIPNRTYEVGLTRFA 93

Query: 114 DLTNEEYRARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCG 173
           DLTN+E+RA +L  +  R  +    K  +Y   +GD LPD +DWR KGAV  VKDQG CG
Sbjct: 94  DLTNDEFRAIYLRSKMER--TRVPVKGEKYLYKVGDSLPDAIDWRAKGAVNPVKDQGSCG 153

Query: 174 SCWAFSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGID 233
           SCWAFS + AVEGINQI TGELISLSEQELVDCD S+N GC GGLMDYAF+FII+NGGID
Sbjct: 154 SCWAFSAIGAVEGINQIKTGELISLSEQELVDCDTSYNDGCGGGLMDYAFKFIIENGGID 213

Query: 234 TDEDYPYKGRD-GACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRA 293
           T+EDYPY   D   C+ ++KN +VVTIDGYEDVP+NDE SLKKA+ANQP+SVAIEAGGRA
Sbjct: 214 TEEDYPYIATDVNVCNSDKKNTRVVTIDGYEDVPQNDEKSLKKALANQPISVAIEAGGRA 273

Query: 294 FQLYQSGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANS 353
           FQLY SGVFTG CGT+LDHGVVAVGYG++ G DYWIVRNSWG +WGE+GY +LERN+  S
Sbjct: 274 FQLYTSGVFTGTCGTSLDHGVVAVGYGSEGGQDYWIVRNSWGSNWGESGYFKLERNIKES 333

Query: 354 TTGKCGIAVEPSYPIK-SGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGST 413
            +GKCG+A+  SYP K SGSNPPKP A  PSPV     CD+  +C   STCCC+Y+Y   
Sbjct: 334 -SGKCGVAMMASYPTKSSGSNPPKPPA--PSPV----VCDKSNTCPAKSTCCCLYEYNGK 393

Query: 414 CFAWGCCPLESATCCDDHYSCCPHEYPVCDLEAGTCR 448
           C++WGCCP ESATCCDD  SCCP  YPVCDL+A TCR
Sbjct: 394 CYSWGCCPYESATCCDDGSSCCPQSYPVCDLKANTCR 421

[Close]

BLAST of Cmc01g0003571 vs. TAIR 10
Match: AT4G36880.1 (cysteine proteinase1 )

HSP 1 Score: 443.4 bits (1139), Expect = 2.5e-124
Identity = 228/370 (61.62%), Postives = 279/370 (75.41%), Query Frame = 0


Query: 12  LALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVAALYESWLVHHG 71
           L+L L     S AS     SII++    HL +P    SD   RTDEEV ++Y  W   HG
Sbjct: 8   LSLLLLYVVVSLASGDE--SIIND----HLQLP----SDGKWRTDEEVRSIYLQWSAEHG 67

Query: 72  KAYN----ALGEKERRFEIFKDNLMFIDEHNRESR--TYKVGLTRFADLTNEEYRARFLG 131
           K  N     + ++++RF IFKDNL FID HN  ++  TYK+GLT+F DLTN+EYR  +LG
Sbjct: 68  KTNNNNNGIINDQDKRFNIFKDNLRFIDLHNENNKNATYKLGLTKFTDLTNDEYRKLYLG 127

Query: 132 GRFSRKPSLSAAK--SGRYAAAL-GDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTVAA 191
            R      ++ AK  + +Y+AA+ G ++P+ VDWR+KGAV  +KDQG CGSCWAFST AA
Sbjct: 128 ARTEPARRIAKAKNVNQKYSAAVNGKEVPETVDWRQKGAVNPIKDQGTCGSCWAFSTTAA 187

Query: 192 VEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYKGR 251
           VEGIN+IVTGELISLSEQELVDCDKS+N GCNGGLMDYAFQFI+ NGG++T++DYPY+G 
Sbjct: 188 VEGINKIVTGELISLSEQELVDCDKSYNQGCNGGLMDYAFQFIMKNGGLNTEKDYPYRGF 247

Query: 252 DGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTG 311
            G C+   KN++VV+IDGYEDVP  DE++LKKA++ QPVSVAIEAGGR FQ YQSG+FTG
Sbjct: 248 GGKCNSFLKNSRVVSIDGYEDVPTKDETALKKAISYQPVSVAIEAGGRIFQHYQSGIFTG 307

Query: 312 RCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAVEP 371
            CGTNLDH VVAVGYG++NG DYWIVRNSWG  WGE GYIR+ERN+A S +GKCGIAVE 
Sbjct: 308 SCGTNLDHAVVAVGYGSENGVDYWIVRNSWGPRWGEEGYIRMERNLAASKSGKCGIAVEA 367

Query: 372 SYPIKSGSNP 373
           SYP+K   NP
Sbjct: 368 SYPVKYSPNP 367

[Close]

BLAST of Cmc01g0003571 vs. TAIR 10
Match: AT1G09850.1 (xylem bark cysteine peptidase 3 )

HSP 1 Score: 439.1 bits (1128), Expect = 4.7e-123
Identity = 211/393 (53.69%), Postives = 270/393 (68.70%), Query Frame = 0


Query: 55  TDEEVAALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNR-ESRTYKVGLTRFAD 114
           + ++++ L++ W   HGK Y +  E+++R +IFKDN  F+ +HN   + TY + L  FAD
Sbjct: 24  SSDDISELFDDWCQKHGKTYGSEEERQQRIQIFKDNHDFVTQHNLITNATYSLSLNAFAD 83

Query: 115 LTNEEYRARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGS 174
           LT+ E++A  LG   S    + A+K      ++   +PD VDWRKKGAV NVKDQG CG+
Sbjct: 84  LTHHEFKASRLGLSVSAPSVIMASKGQSLGGSV--KVPDSVDWRKKGAVTNVKDQGSCGA 143

Query: 175 CWAFSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDT 234
           CW+FS   A+EGINQIVTG+LISLSEQEL+DCDKS+N GCNGGLMDYAF+F+I N GIDT
Sbjct: 144 CWSFSATGAMEGINQIVTGDLISLSEQELIDCDKSYNAGCNGGLMDYAFEFVIKNHGIDT 203

Query: 235 DEDYPYKGRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQ 294
           ++DYPY+ RDG C  ++   KVVTID Y  V  NDE +L +AVA QPVSV I    RAFQ
Sbjct: 204 EKDYPYQERDGTCKKDKLKQKVVTIDSYAGVKSNDEKALMEAVAAQPVSVGICGSERAFQ 263

Query: 295 LYQSGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTT 354
           LY SG+F+G C T+LDH V+ VGYG+ NG DYWIV+NSWGK WG +G++ ++RN  NS  
Sbjct: 264 LYSSGIFSGPCSTSLDHAVLIVGYGSQNGVDYWIVKNSWGKSWGMDGFMHMQRNTENS-D 323

Query: 355 GKCGIAVEPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFA 414
           G CGI +  SYPIK+  NPP P  SPP     PT+C+ +  C  G TCCC  +    CF+
Sbjct: 324 GVCGINMLASYPIKTHPNPPPP--SPPG----PTKCNLFTYCSSGETCCCARELFGLCFS 383

Query: 415 WGCCPLESATCCDDHYSCCPHEYPVCDLEAGTC 447
           W CC +ESA CC D   CCPH+YPVCD     C
Sbjct: 384 WKCCEIESAVCCKDGRHCCPHDYPVCDTTRSLC 407

The following BLAST results are available for this feature:

BLAST of Cmc01g0003571 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008454976.1	4.3e-267	99.56	PREDICTED: low-temperature-induced cysteine proteinase-like [Cucumis melo]	[more]
KAA0031322.1	9.8e-264	97.60	low-temperature-induced cysteine proteinase-like [Cucumis melo var. makuwa] >TYK...	[more]
XP_004136967.1	1.2e-253	94.67	low-temperature-induced cysteine proteinase [Cucumis sativus] >KGN43904.1 hypoth...	[more]
XP_038889032.1	2.3e-241	90.49	low-temperature-induced cysteine proteinase-like [Benincasa hispida]	[more]
XP_022147795.1	3.8e-223	82.89	low-temperature-induced cysteine proteinase-like [Momordica charantia]	[more]

BLAST of Cmc01g0003571 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P43297	1.9e-177	68.05	Cysteine proteinase RD21A OS=Arabidopsis thaliana OX=3702 GN=RD21A PE=1 SV=1	[more]
Q9FMH8	5.7e-174	67.04	Probable cysteine protease RD21B OS=Arabidopsis thaliana OX=3702 GN=RD21B PE=1 S...	[more]
P25776	1.0e-170	69.35	Oryzain alpha chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0650000 PE=...	[more]
Q9LT78	1.1e-153	67.00	Probable cysteine protease RD21C OS=Arabidopsis thaliana OX=3702 GN=RD21C PE=1 S...	[more]
P25777	1.5e-150	60.79	Oryzain beta chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0670200 PE=1...	[more]

BLAST of Cmc01g0003571 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BZW8	2.1e-267	99.56	low-temperature-induced cysteine proteinase-like OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A5A7SJU5	4.7e-264	97.60	Low-temperature-induced cysteine proteinase-like OS=Cucumis melo var. makuwa OX=...	[more]
A0A0A0K4N3	5.8e-254	94.67	Cysteine protease OS=Cucumis sativus OX=3659 GN=Csa_7G073400 PE=3 SV=1	[more]
A0A6J1D149	1.8e-223	82.89	low-temperature-induced cysteine proteinase-like OS=Momordica charantia OX=3673 ...	[more]
A0A6J1EI11	7.2e-220	83.30	low-temperature-induced cysteine proteinase-like OS=Cucurbita moschata OX=3662 G...	[more]

BLAST of Cmc01g0003571 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G47128.1	1.4e-178	68.05	Granulin repeat cysteine protease family protein	[more]
AT5G43060.1	4.1e-175	67.04	Granulin repeat cysteine protease family protein	[more]
AT3G19390.1	8.0e-155	67.00	Granulin repeat cysteine protease family protein	[more]
AT4G36880.1	2.5e-124	61.62	cysteine proteinase1	[more]
AT1G09850.1	4.7e-123	53.69	xylem bark cysteine peptidase 3	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000668	Peptidase C1A, papain C-terminal	PRINTS	PR00705	PAPAIN	coord: 168..183 score: 66.81 coord: 310..320 score: 57.41 coord: 325..331 score: 73.06
IPR000668	Peptidase C1A, papain C-terminal	SMART	SM00645	pept_c1	coord: 150..366 e-value: 3.2E-128 score: 441.9
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 150..366 e-value: 1.0E-85 score: 287.3
IPR013201	Cathepsin propeptide inhibitor domain (I29)	SMART	SM00848	Inhibitor_I29_2	coord: 63..119 e-value: 3.0E-25 score: 99.9
IPR013201	Cathepsin propeptide inhibitor domain (I29)	PFAM	PF08246	Inhibitor_I29	coord: 63..119 e-value: 1.8E-17 score: 63.5
IPR000118	Granulin	SMART	SM00277	GRAN_2	coord: 389..446 e-value: 4.7E-26 score: 102.6
IPR000118	Granulin	PFAM	PF00396	Granulin	coord: 400..446 e-value: 4.3E-10 score: 39.8
IPR037277	Granulin superfamily	GENE3D	2.10.25.160	Granulin	coord: 386..449 e-value: 5.9E-13 score: 50.9
None	No IPR available	GENE3D	3.90.70.10	Cysteine proteinases	coord: 45..368 e-value: 4.6E-123 score: 412.8
None	No IPR available	PANTHER	PTHR12411:SF749	CYSTEINE PROTEASE	coord: 44..396
None	No IPR available	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 44..396
None	No IPR available	SUPERFAMILY	57277	Granulin repeat	coord: 386..420
IPR025660	Cysteine peptidase, histidine active site	PROSITE	PS00639	THIOL_PROTEASE_HIS	coord: 308..318
IPR025661	Cysteine peptidase, asparagine active site	PROSITE	PS00640	THIOL_PROTEASE_ASN	coord: 325..344
IPR000169	Cysteine peptidase, cysteine active site	PROSITE	PS00139	THIOL_PROTEASE_CYS	coord: 168..179
IPR039417	Papain-like cysteine endopeptidase	CDD	cd02248	Peptidase_C1A	coord: 151..365 e-value: 1.58911E-112 score: 329.585
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 55..366

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cmc01g0003571.1	Cmc01g0003571.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005615	extracellular space
cellular_component	GO:0005764	lysosome
molecular_function	GO:0032440	2-alkenal reductase [NAD(P)+] activity
molecular_function	GO:0004197	cysteine-type endopeptidase activity
molecular_function	GO:0008234	cysteine-type peptidase activity