CmaCh20G010840 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh20G010840
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionterpene synthase 10-like
LocationCma_Chr20: 8323844 .. 8326857 (+)
RNA-Seq ExpressionCmaCh20G010840
SyntenyCmaCh20G010840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTTTCTTTGTTAATGGCTCTTCACCAATTTTTTACTTCTTTTACGAGTCTTTTCATCAAACGAGTCTCTTTTGATCTCAATTGTTCATTTGTCCCTCCCGTTCTTAAAGTACATGGCGGTGTAAGAATGTCAAGTCAAACTATTGTACGACGGTCTGGAAATTACAAACCTCCTATTTGGAAACTTGAGTTCATTGAGTCATTAAAAAGTGAATTTAAGGTATGAATGATGATAGAAATCTTTTGATTGTTTACTCTTTTTCGTTTTATTTACATTTAATTTTGAAATGAGTTTGACAATTACTTGTGGATATGTATGATGTTTTCAGGGAGAAATGTACGTAGAACATTTGAATGAGCTAAAGGAAGAGATCGATATTATCATGAACCAAATAATAGACGACCCTTTAAAGCAACTCGAGCTGATTGATACTTTACAACGACTTGGAATCTCATATCATTTTGAGAATGAGATTAAGGATATGTTGAAAAGAACATATGAGAAGAGTTATGAAAGTGATGATTGGAAAAAGAATAATCTGCATGCAACATCACTTGAATTTAGACTTCTAAGACAACATGGATTCAATGTTTCACAAGGTATCAACTTTGTTCAACACTTAATATTACTTGTGGATATGTTTTCATGATTTTTTTTTTCTTTTTTTGCACAATATAATGGAATAGCAATTCATTGGATATCAAATTCACGAGCATGTGAAACTATCAACATGAAAATTTAGTATCTTAGTCATGCTGCATTAAACCGTGCGTATTTATCAACAACTTTTTGTATTTCAATGACAGAGGTTTTCAATAACTTCAAGGATGAGATGAAAAACTTTAGCACACGACTCTATAAAGATTTGAATGGAATGTTATCTTTGTATGAAGCTTCGTTCTTATGTATAGAAGGTGAAAACATTTTAGAGGCAGCAAAACATTTCACAATCAACCATTTGGAAAAATATATGAGATCGTGCAAGGATGAACATGAAGCATCCATTATTAGACATGCGTTGGAGTTCCCCTTGTATTGGCAAATATCAAGATTGGATACAAGATGGTTCATTGATATCTACGAGAGAAAAGTGGATATGAATCCTATTCTTCTCGAAATTGCCAAATTAGATTTCAATCAAGTTCAATTTATCCACCAAGAAGACGTGAAATATGCATCAAGGTAAGATTATTATAAACATATACATATTTAGAGCATAGACAATAATGATTTATGATGTTCTTTTATCGAAATTTTGTTAAATCACAGATCAACTCAAAAATTTTCTTTTCCTTTGAAAAAATTGATGATTTTTTTATTTTTTATTTACATTTTTAAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTTTAAATTTATAGATTGTTATAAATTTAATTATATCAATACTGGAACATTCCCTCTTCCGGTTAGAACGTTGATACAGTCGTTGAGAAATTTCTGAAAGGCTAATCCAATCTCGATATAGCCTTTACGCAATCTTAATCTAATTCATTCCTTCATTGAATTGGATTTTAATTATATTAACATGTTTACAAACTCCAAGAACAGGTGGTGGAGGAGCACTGGATTTAGCGAAAAGTTCAGCTTTGCAAGAGATAGATTGATGGAAAATTTCTTTTGGACAGTGGGTGTTGGATTTGAACCCGAGCTCTCATACTTTAGAAGAATGGCCACAAAAGTTAATGCATTAATAACAACAATAGATGATATTTATGATGTCTATGGCACATTAGATGAACTCGAGCTCTTTACTGACGCGGTAGAGAGGTAAGCAAACGATAAATCTATGACAATAAGAAAAAGTTTAATCTCGGTGTTAATTTCTATTTGTTATATTATTTTGTTTACCAATTCATTTATTTTTTAATAGATAATTAAATTTACCATATTAAAATCTCAATATTTTTTTTTTGATATATAATCCTCTTCTAAAATATGATTTCAGGTGGGACGTTGGTACAATGGATCAATTACCTGACTATATGAAAATATGTTTTTTTAGTCTCCACAATTCTATCAATGAGATGGCTTTTGAAGCACTGAGAGACAAAGGAATTAATGTGCTTAAGTACCTTAAGAAGGCGGTATGAATTTAATTATGATAAGTATTTCTAACTTTTCTTTTGATTAAGATCAACAAAATTGTTACGTAATTTATTTATATGATACTTAATTTTATAGTGGGCAGATTTGTGCCAAGCTTATATGTTGGAGGCTAAGTGGTATCACACCAGCTATAAACCAACTTTAGAAGAATATTTAGACAATGCATGGATTTCAATATCAGGACCAGTTATATTAGTTCATGCATATGCTTTCGTGACATCCCCAACGCTCCAAGACATGGAAAGCTTGGTAGAATATGCTGATATAATTCGTTGGTCTTCCACAATTTTGCGACTTGCTAATGATCTAGGAACATCATCGGTATGTAAACAAGAAGCCTTATTTAAAAGGAACTTTTTTTTTAACTACGATAATTGAAAATTAAGTATGTGAGTAGGTAACTATCTTCGAGTTAATGAAATTCCGTGCATGTTATTAAATTTAACATTTTGAAATTTTTATCAACTAATATGATCCTATGATGGATAAATTTCATTCAGGATGAGTTAGAAAGAGGTGATATTCTGAAATCGATACAATGTTACATGAATGACACGGGAGCCTCGGAGAGTAAAGCTCGTGAGCATGTAAAGCGTTTGATTGATGAGACATGGAAGAAGTTAAATAAGGCTGAAGTTGAGAATTCTATCTTCTCTCAAGTGTTTATCGAGAGAGCTAAAAATCTTGCTAGAATGGCACAATGCGTGTATCAATATGGAGATGGACATGGCATGGGATACCAAGAAATAAACGATCGTGTAATGTCTCTACTTATCCGACCCATTTCCACACATTCATATAATGAATAATGATCAGAGTTTTTCGAATTGTTATAACAGAAGCGGAAACAAAATCGATTGCAC

mRNA sequence

GTTTTTCTTTGTTAATGGCTCTTCACCAATTTTTTACTTCTTTTACGAGTCTTTTCATCAAACGAGTCTCTTTTGATCTCAATTGTTCATTTGTCCCTCCCGTTCTTAAAGTACATGGCGGTGTAAGAATGTCAAGTCAAACTATTGTACGACGGTCTGGAAATTACAAACCTCCTATTTGGAAACTTGAGTTCATTGAGTCATTAAAAAGTGAATTTAAGGGAGAAATGTACGTAGAACATTTGAATGAGCTAAAGGAAGAGATCGATATTATCATGAACCAAATAATAGACGACCCTTTAAAGCAACTCGAGCTGATTGATACTTTACAACGACTTGGAATCTCATATCATTTTGAGAATGAGATTAAGGATATGTTGAAAAGAACATATGAGAAGAGTTATGAAAGTGATGATTGGAAAAAGAATAATCTGCATGCAACATCACTTGAATTTAGACTTCTAAGACAACATGGATTCAATGTTTCACAAGAGGTTTTCAATAACTTCAAGGATGAGATGAAAAACTTTAGCACACGACTCTATAAAGATTTGAATGGAATGTTATCTTTGTATGAAGCTTCGTTCTTATGTATAGAAGGTGAAAACATTTTAGAGGCAGCAAAACATTTCACAATCAACCATTTGGAAAAATATATGAGATCGTGCAAGGATGAACATGAAGCATCCATTATTAGACATGCGTTGGAGTTCCCCTTGTATTGGCAAATATCAAGATTGGATACAAGATGGTTCATTGATATCTACGAGAGAAAAGTGGATATGAATCCTATTCTTCTCGAAATTGCCAAATTAGATTTCAATCAAGTTCAATTTATCCACCAAGAAGACGTGAAATATGCATCAAGGTGGTGGAGGAGCACTGGATTTAGCGAAAAGTTCAGCTTTGCAAGAGATAGATTGATGGAAAATTTCTTTTGGACAGTGGGTGTTGGATTTGAACCCGAGCTCTCATACTTTAGAAGAATGGCCACAAAAGTTAATGCATTAATAACAACAATAGATGATATTTATGATGTCTATGGCACATTAGATGAACTCGAGCTCTTTACTGACGCGGTAGAGAGGTGGGACGTTGGTACAATGGATCAATTACCTGACTATATGAAAATATGTTTTTTTAGTCTCCACAATTCTATCAATGAGATGGCTTTTGAAGCACTGAGAGACAAAGGAATTAATGTGCTTAAGTACCTTAAGAAGGCGTGGGCAGATTTGTGCCAAGCTTATATGTTGGAGGCTAAGTGGTATCACACCAGCTATAAACCAACTTTAGAAGAATATTTAGACAATGCATGGATTTCAATATCAGGACCAGTTATATTAGTTCATGCATATGCTTTCGTGACATCCCCAACGCTCCAAGACATGGAAAGCTTGGTAGAATATGCTGATATAATTCGTTGGTCTTCCACAATTTTGCGACTTGCTAATGATCTAGGAACATCATCGGATGAGTTAGAAAGAGGTGATATTCTGAAATCGATACAATGTTACATGAATGACACGGGAGCCTCGGAGAGTAAAGCTCGTGAGCATGTAAAGCGTTTGATTGATGAGACATGGAAGAAGTTAAATAAGGCTGAAGTTGAGAATTCTATCTTCTCTCAAGTGTTTATCGAGAGAGCTAAAAATCTTGCTAGAATGGCACAATGCGTGTATCAATATGGAGATGGACATGGCATGGGATACCAAGAAATAAACGATCGTGTAATGTCTCTACTTATCCGACCCATTTCCACACATTCATATAATGAATAATGATCAGAGTTTTTCGAATTGTTATAACAGAAGCGGAAACAAAATCGATTGCAC

Coding sequence (CDS)

ATGGCTCTTCACCAATTTTTTACTTCTTTTACGAGTCTTTTCATCAAACGAGTCTCTTTTGATCTCAATTGTTCATTTGTCCCTCCCGTTCTTAAAGTACATGGCGGTGTAAGAATGTCAAGTCAAACTATTGTACGACGGTCTGGAAATTACAAACCTCCTATTTGGAAACTTGAGTTCATTGAGTCATTAAAAAGTGAATTTAAGGGAGAAATGTACGTAGAACATTTGAATGAGCTAAAGGAAGAGATCGATATTATCATGAACCAAATAATAGACGACCCTTTAAAGCAACTCGAGCTGATTGATACTTTACAACGACTTGGAATCTCATATCATTTTGAGAATGAGATTAAGGATATGTTGAAAAGAACATATGAGAAGAGTTATGAAAGTGATGATTGGAAAAAGAATAATCTGCATGCAACATCACTTGAATTTAGACTTCTAAGACAACATGGATTCAATGTTTCACAAGAGGTTTTCAATAACTTCAAGGATGAGATGAAAAACTTTAGCACACGACTCTATAAAGATTTGAATGGAATGTTATCTTTGTATGAAGCTTCGTTCTTATGTATAGAAGGTGAAAACATTTTAGAGGCAGCAAAACATTTCACAATCAACCATTTGGAAAAATATATGAGATCGTGCAAGGATGAACATGAAGCATCCATTATTAGACATGCGTTGGAGTTCCCCTTGTATTGGCAAATATCAAGATTGGATACAAGATGGTTCATTGATATCTACGAGAGAAAAGTGGATATGAATCCTATTCTTCTCGAAATTGCCAAATTAGATTTCAATCAAGTTCAATTTATCCACCAAGAAGACGTGAAATATGCATCAAGGTGGTGGAGGAGCACTGGATTTAGCGAAAAGTTCAGCTTTGCAAGAGATAGATTGATGGAAAATTTCTTTTGGACAGTGGGTGTTGGATTTGAACCCGAGCTCTCATACTTTAGAAGAATGGCCACAAAAGTTAATGCATTAATAACAACAATAGATGATATTTATGATGTCTATGGCACATTAGATGAACTCGAGCTCTTTACTGACGCGGTAGAGAGGTGGGACGTTGGTACAATGGATCAATTACCTGACTATATGAAAATATGTTTTTTTAGTCTCCACAATTCTATCAATGAGATGGCTTTTGAAGCACTGAGAGACAAAGGAATTAATGTGCTTAAGTACCTTAAGAAGGCGTGGGCAGATTTGTGCCAAGCTTATATGTTGGAGGCTAAGTGGTATCACACCAGCTATAAACCAACTTTAGAAGAATATTTAGACAATGCATGGATTTCAATATCAGGACCAGTTATATTAGTTCATGCATATGCTTTCGTGACATCCCCAACGCTCCAAGACATGGAAAGCTTGGTAGAATATGCTGATATAATTCGTTGGTCTTCCACAATTTTGCGACTTGCTAATGATCTAGGAACATCATCGGATGAGTTAGAAAGAGGTGATATTCTGAAATCGATACAATGTTACATGAATGACACGGGAGCCTCGGAGAGTAAAGCTCGTGAGCATGTAAAGCGTTTGATTGATGAGACATGGAAGAAGTTAAATAAGGCTGAAGTTGAGAATTCTATCTTCTCTCAAGTGTTTATCGAGAGAGCTAAAAATCTTGCTAGAATGGCACAATGCGTGTATCAATATGGAGATGGACATGGCATGGGATACCAAGAAATAAACGATCGTGTAATGTCTCTACTTATCCGACCCATTTCCACACATTCATATAATGAATAA

Protein sequence

MALHQFFTSFTSLFIKRVSFDLNCSFVPPVLKVHGGVRMSSQTIVRRSGNYKPPIWKLEFIESLKSEFKGEMYVEHLNELKEEIDIIMNQIIDDPLKQLELIDTLQRLGISYHFENEIKDMLKRTYEKSYESDDWKKNNLHATSLEFRLLRQHGFNVSQEVFNNFKDEMKNFSTRLYKDLNGMLSLYEASFLCIEGENILEAAKHFTINHLEKYMRSCKDEHEASIIRHALEFPLYWQISRLDTRWFIDIYERKVDMNPILLEIAKLDFNQVQFIHQEDVKYASRWWRSTGFSEKFSFARDRLMENFFWTVGVGFEPELSYFRRMATKVNALITTIDDIYDVYGTLDELELFTDAVERWDVGTMDQLPDYMKICFFSLHNSINEMAFEALRDKGINVLKYLKKAWADLCQAYMLEAKWYHTSYKPTLEEYLDNAWISISGPVILVHAYAFVTSPTLQDMESLVEYADIIRWSSTILRLANDLGTSSDELERGDILKSIQCYMNDTGASESKAREHVKRLIDETWKKLNKAEVENSIFSQVFIERAKNLARMAQCVYQYGDGHGMGYQEINDRVMSLLIRPISTHSYNE
Homology
BLAST of CmaCh20G010840 vs. ExPASy Swiss-Prot
Match: B9T536 (Terpene synthase 10 OS=Ricinus communis OX=3988 GN=TPS10 PE=2 SV=1)

HSP 1 Score: 713.8 bits (1841), Expect = 1.6e-204
Identity = 337/545 (61.83%), Postives = 439/545 (80.55%), Query Frame = 0

Query: 39  MSSQTIVRRSGNYKPPIWKLEFIESLKSEFKGEMYVEHLNELKEEIDIIMNQIIDDPLKQ 98
           MS++TI+RRS NY+PPIW  +F++SLKSEF GE+ ++ +++LKE++ +++N+ +  P  Q
Sbjct: 6   MSNETIIRRSANYRPPIWDFDFVQSLKSEFVGELNIKRIDKLKEDVKMMLNKTM-APSDQ 65

Query: 99  LELIDTLQRLGISYHFENEIKDMLKRTYEKSYESDDWKKNNLHATSLEFRLLRQHGFNVS 158
            ELIDTLQRLG++YHF +EIK ++K  Y      + W K +LH  +L+FRLLRQHG+N+S
Sbjct: 66  FELIDTLQRLGLAYHFGDEIKRIVKSIYNSHRNDNTWMKEDLHTIALQFRLLRQHGYNIS 125

Query: 159 QEVFNNFKDEMKNFSTRLYKDLNGMLSLYEASFLCIEGENILEAAKHFTINHLEKYMRSC 218
           QE+F+ F+DE+ NF   L++D+ GMLSLYEAS+L  EGENILE A+ F  + L+KY++  
Sbjct: 126 QEIFDIFRDELGNFKECLHEDIEGMLSLYEASYLLEEGENILEVAREFAASCLKKYIQVN 185

Query: 219 KDEHEASIIRHALEFPLYWQISRLDTRWFIDIYERKVDMNPILLEIAKLDFNQVQFIHQE 278
           KD+  + I+ H+LE PL+W++ RL+TRWFIDIYE+K  MNP+LLE+AKLDFN VQ  + E
Sbjct: 186 KDQLLSMIVSHSLEVPLHWRMPRLETRWFIDIYEKKQGMNPLLLELAKLDFNNVQATYHE 245

Query: 279 DVKYASRWWRSTGFSEKFSFARDRLMENFFWTVGVGFEPELSYFRRMATKVNALITTIDD 338
           D+KY + WWR+TG  EK SFARDRLMENF WTVGV F P+  YFRR++TKVN+LIT IDD
Sbjct: 246 DLKYVTSWWRNTGLGEKLSFARDRLMENFLWTVGVNFPPQFGYFRRISTKVNSLITVIDD 305

Query: 339 IYDVYGTLDELELFTDAVERWDVGTMDQLPDYMKICFFSLHNSINEMAFEALRDKGINVL 398
           IYDVYGTLDEL+LFT+AVERWDV  MDQLP+YMK+CF +LHNSINEMA++ALR++G +++
Sbjct: 306 IYDVYGTLDELQLFTNAVERWDVNAMDQLPEYMKLCFLALHNSINEMAYDALREQGFHII 365

Query: 399 KYLKKAWADLCQAYMLEAKWYHTSYKPTLEEYLDNAWISISGPVILVHAYAFVTSP-TLQ 458
            YLKKAWADLC++Y++EAKWY+  Y PTL+EY+DNAWISIS PVILVHAY    SP T +
Sbjct: 366 PYLKKAWADLCKSYLVEAKWYYIGYTPTLQEYMDNAWISISAPVILVHAYFLEGSPITNE 425

Query: 459 DMESLVEYADIIRWSSTILRLANDLGTSSDELERGDILKSIQCYMNDTGASESKAREHVK 518
            ++SL EY DII+WSS ILR A+DLGTSSDEL+RGD  KSIQCY+ +TG SE KAREH++
Sbjct: 426 ALKSLKEYPDIIQWSSMILRFADDLGTSSDELKRGDNPKSIQCYIYETGVSELKAREHIQ 485

Query: 519 RLIDETWKKLNK-AEVENSIFSQVFIERAKNLARMAQCVYQYGDGHGMGYQEINDRVMSL 578
            LI ETWKK+NK  E  +S FS++FIE A NLARMAQC+YQ+GDGHG+   E  D V+SL
Sbjct: 486 YLIGETWKKINKEREYIDSPFSKIFIEVATNLARMAQCMYQHGDGHGIEDGETKDHVLSL 545

Query: 579 LIRPI 582
           L++PI
Sbjct: 546 LVKPI 549

BLAST of CmaCh20G010840 vs. ExPASy Swiss-Prot
Match: Q93X23 (Myrcene synthase, chloroplastic OS=Quercus ilex OX=58334 PE=1 SV=1)

HSP 1 Score: 701.0 bits (1808), Expect = 1.1e-200
Identity = 329/543 (60.59%), Postives = 428/543 (78.82%), Query Frame = 0

Query: 40  SSQTIVRRSGNYKPPIWKLEFIESLKSEFKGEMYVEHLNELKEEIDIIMNQIIDDPLKQL 99
           +S  I+RRS NY+P IW  ++IESL+ E+ GE     +N LKE++ +++++++ +PL+QL
Sbjct: 51  TSPDILRRSANYQPSIWNHDYIESLRIEYVGETCTRQINVLKEQVRMMLHKVV-NPLEQL 110

Query: 100 ELIDTLQRLGISYHFENEIKDMLKRTYEKSYESDDWKKNNLHATSLEFRLLRQHGFNVSQ 159
           ELI+ LQRLG+SYHFE EIK +L   Y   +  D WK  NL+AT+L+FRLLRQHG++VSQ
Sbjct: 111 ELIEILQRLGLSYHFEEEIKRILDGVYNNDHGGDTWKAENLYATALKFRLLRQHGYSVSQ 170

Query: 160 EVFNNFKDEMKNFSTRLYKDLNGMLSLYEASFLCIEGENILEAAKHFTINHLEKYMRSCK 219
           EVFN+FKDE  +F   L +D  GMLSLYEASF  IEGENILE A+ F+  HLE+Y++  K
Sbjct: 171 EVFNSFKDERGSFKACLCEDTKGMLSLYEASFFLIEGENILEEARDFSTKHLEEYVKQNK 230

Query: 220 DEHEASIIRHALEFPLYWQISRLDTRWFIDIYERKVDMNPILLEIAKLDFNQVQFIHQED 279
           +++ A+++ H+LEFPL+W++ RL+ RWFI+IY    D+NPILLE A+LDFN VQ  HQ D
Sbjct: 231 EKNLATLVNHSLEFPLHWRMPRLEARWFINIYRHNQDVNPILLEFAELDFNIVQAAHQAD 290

Query: 280 VKYASRWWRSTGFSEKFSFARDRLMENFFWTVGVGFEPELSYFRRMATKVNALITTIDDI 339
           +K  S WW+STG  E  SFARDR +ENFFWTVG+ F+P+  Y RRM TKV ALITTIDD+
Sbjct: 291 LKQVSTWWKSTGLVENLSFARDRPVENFFWTVGLIFQPQFGYCRRMFTKVFALITTIDDV 350

Query: 340 YDVYGTLDELELFTDAVERWDVGTMDQLPDYMKICFFSLHNSINEMAFEALRDKGINVLK 399
           YDVYGTLDELELFTD VERWD+  MDQLPDYMKICF +LHNS+NEMA + ++++  +++K
Sbjct: 351 YDVYGTLDELELFTDVVERWDINAMDQLPDYMKICFLTLHNSVNEMALDTMKEQRFHIIK 410

Query: 400 YLKKAWADLCQAYMLEAKWYHTSYKPTLEEYLDNAWISISGPVILVHAYAFVTSP-TLQD 459
           YLKKAW DLC+ Y++EAKWY   Y+P+L+EY++NAWISI  P ILVHAY FVT+P T + 
Sbjct: 411 YLKKAWVDLCRYYLVEAKWYSNKYRPSLQEYIENAWISIGAPTILVHAYFFVTNPITKEA 470

Query: 460 MESLVEYADIIRWSSTILRLANDLGTSSDELERGDILKSIQCYMNDTGASESKAREHVKR 519
           ++ L EY +IIRWSS I RLA+DLGTS+DEL+RGD+ K+IQCYMN+TGASE  ARE++K 
Sbjct: 471 LDCLEEYPNIIRWSSIIARLADDLGTSTDELKRGDVPKAIQCYMNETGASEEGAREYIKY 530

Query: 520 LIDETWKKLNKAEVENSIFSQVFIERAKNLARMAQCVYQYGDGHGMGYQEINDRVMSLLI 579
           LI  TWKK+NK    +S FS +FIE A NLARMAQC+YQ+GDGHG+G +E  DR++SLLI
Sbjct: 531 LISATWKKMNKDRAASSPFSHIFIEIALNLARMAQCLYQHGDGHGLGNRETKDRILSLLI 590

Query: 580 RPI 582
           +PI
Sbjct: 591 QPI 592

BLAST of CmaCh20G010840 vs. ExPASy Swiss-Prot
Match: Q6PWU2 ((-)-alpha-terpineol synthase OS=Vitis vinifera OX=29760 PE=1 SV=1)

HSP 1 Score: 676.8 bits (1745), Expect = 2.2e-193
Identity = 319/564 (56.56%), Postives = 427/564 (75.71%), Query Frame = 0

Query: 23  NCSFVPPVLKVHGGVRMSSQTIVRRSGNYKPPIWKLEFIESLKSEFKGEMYVEHLNELKE 82
           +C   P  +KV  G     + IVRR+ NY P IW  ++++SL+S++ GE Y   L++LK 
Sbjct: 26  SCKASPRGIKVKIGNSNCEEIIVRRTANYHPTIWDYDYVQSLRSDYVGETYTRRLDKLKR 85

Query: 83  EIDIIMNQIIDDPLKQLELIDTLQRLGISYHFENEIKDMLKRTYEKSYESDDWKKNNLHA 142
           ++  ++ + +  PL QLELID LQRLGI YHF++EIK +L   Y +    ++W+K++L+A
Sbjct: 86  DVKPMLGK-VKKPLDQLELIDVLQRLGIYYHFKDEIKRILNGIYNQYNRHEEWQKDDLYA 145

Query: 143 TSLEFRLLRQHGFNVSQEVFNNFKDEMKNFSTRLYKDLNGMLSLYEASFLCIEGENILEA 202
           T+LEFRLLRQHG++V Q+VF+ FKD+  +F   L +D+ GML LYEAS+LC++GE+ +E 
Sbjct: 146 TALEFRLLRQHGYDVPQDVFSRFKDDTGSFKACLCEDMKGMLCLYEASYLCVQGESTMEQ 205

Query: 203 AKHFTINHLEKYMRSCKDEHEASIIRHALEFPLYWQISRLDTRWFIDIYERKVDMNPILL 262
           A+ F   HL K +    D++ A  ++HALE PL+W++ RL+ RWFID+YE++ DMNPILL
Sbjct: 206 ARDFAHRHLGKGLEQNIDQNLAIEVKHALELPLHWRMPRLEARWFIDVYEKRQDMNPILL 265

Query: 263 EIAKLDFNQVQFIHQEDVKYASRWWRSTGFSEKFSFARDRLMENFFWTVGVGFEPELSYF 322
           E AKLDFN VQ  HQED+++ S WW ST   EK +FARDRLMENF WTVGV FEP+  Y 
Sbjct: 266 EFAKLDFNMVQATHQEDLRHMSSWWSSTRLGEKLNFARDRLMENFLWTVGVIFEPQYGYC 325

Query: 323 RRMATKVNALITTIDDIYDVYGTLDELELFTDAVERWDVGTMDQLPDYMKICFFSLHNSI 382
           RRM+TKVN LIT IDD+YDVYGT+DELELFTD V+RWD+  MD LP+YMK+CF +L+NS 
Sbjct: 326 RRMSTKVNTLITIIDDVYDVYGTMDELELFTDVVDRWDINAMDPLPEYMKLCFLALYNST 385

Query: 383 NEMAFEALRDKGINVLKYLKKAWADLCQAYMLEAKWYHTSYKPTLEEYLDNAWISISGPV 442
           NEMA++AL++ G++++ YL+KAW+DLC++Y+LEAKWY++ Y P+L+EY+ N+WISISGPV
Sbjct: 386 NEMAYDALKEHGLHIVSYLRKAWSDLCKSYLLEAKWYYSRYTPSLQEYISNSWISISGPV 445

Query: 443 ILVHAYAFVTSP-TLQDMESLVEYADIIRWSSTILRLANDLGTSSDELERGDILKSIQCY 502
           ILVHAY  V +P T + ++SL  Y +IIRWSS ILRL++DLGTS DEL+RGD+ KSIQCY
Sbjct: 446 ILVHAYFLVANPITKEALQSLERYHNIIRWSSMILRLSDDLGTSLDELKRGDVPKSIQCY 505

Query: 503 MNDTGASESKAREHVKRLIDETWKKLNKAEVENSIFSQVFIERAKNLARMAQCVYQYGDG 562
           M +TGASE  AR+H   LI ETWKKLN+     S F + FI  A NLARMAQC+YQ+GDG
Sbjct: 506 MYETGASEEDARKHTSYLIGETWKKLNEDGAVESPFPETFIGIAMNLARMAQCMYQHGDG 565

Query: 563 HGMGYQEINDRVMSLLIRPISTHS 586
           HG+ Y E  DRV+SLL+ PI + S
Sbjct: 566 HGIEYGETEDRVLSLLVEPIPSLS 588

BLAST of CmaCh20G010840 vs. ExPASy Swiss-Prot
Match: B6SCF4 (Myrcene synthase, chloroplastic OS=Humulus lupulus OX=3486 PE=1 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 4.4e-173
Identity = 319/609 (52.38%), Postives = 415/609 (68.14%), Query Frame = 0

Query: 1   MALHQFFTSFTSLFIKRVSFDLNCSFVPPV-LKVHGGVRMSSQTIV-----RRSGNYKPP 60
           MA+HQF    + L   R+S D    F P    K         Q  V     RRS NY+P 
Sbjct: 4   MAVHQFAPLLSLLNCSRISSDFGRLFTPKTSTKSRSSTCHPIQCTVVNNTDRRSANYEPS 63

Query: 61  IWKLEFIESLKSEFKGEMYVEHLNELKEEIDIIMNQIIDDPLKQLELIDTLQRLGISYHF 120
           IW  ++I+SL S++KG+ Y   LNELK+E+  +M     + L QL+LIDTLQRLGISYHF
Sbjct: 64  IWSFDYIQSLTSQYKGKSYSSRLNELKKEVK-MMEDGTKECLAQLDLIDTLQRLGISYHF 123

Query: 121 ENEIKDMLKRTYEKSYESDDWKKNNLHATSLEFRLLRQHGFNVSQEVFNNFKDEMKNFST 180
           E+EI  +LKR Y  + +++     NL++T+L+FRLLRQHG+ V+QEVFN FKDE   F T
Sbjct: 124 EDEINTILKRKY-INIQNNINHNYNLYSTALQFRLLRQHGYLVTQEVFNAFKDETGKFKT 183

Query: 181 RLYKDLNGMLSLYEASFLCIEGENILEAAKHFTINHLEKYMRSCKD-------------- 240
            L  D+ G+LSLYEASF  ++ EN+LE A+ F+   L++YM   +               
Sbjct: 184 YLSDDIMGVLSLYEASFYAMKHENVLEEARVFSTECLKEYMMKMEQNKVLLDHDLDHNDN 243

Query: 241 ---EHEASIIRHALEFPLYWQISRLDTRWFIDIYERKVDMNPILLEIAKLDFNQVQFIHQ 300
               H   II HALE PL+W+I+R + RWFID+YE+K DM+  LLE AKLDFN VQ  HQ
Sbjct: 244 FNVNHHVLIINHALELPLHWRITRSEARWFIDVYEKKQDMDSTLLEFAKLDFNMVQSTHQ 303

Query: 301 EDVKYASRWWRSTGFSEKFSFARDRLMENFFWTVGVGFEPELSYFRRMATKVNALITTID 360
           ED+K+ SRWWR +   EK +FARDRLME F W VG+ FEPE SYF+R++ ++  LIT ID
Sbjct: 304 EDLKHLSRWWRHSKLGEKLNFARDRLMEAFLWEVGLKFEPEFSYFKRISARLFVLITIID 363

Query: 361 DIYDVYGTLDELELFTDAVERWDVGTMDQLPDYMKICFFSLHNSINEMAFEALRDKGINV 420
           DIYDVYGTL+ELELFT AVERWDV  +++LP+YMK+ F  LHN+INEMAF+ L D+    
Sbjct: 364 DIYDVYGTLEELELFTKAVERWDVNAINELPEYMKMPFLVLHNTINEMAFDVLGDQNFLN 423

Query: 421 LKYLKKAWADLCQAYMLEAKWYHTSYKPTLEEYLDNAWISISGPVILVHAYAFVTSP-TL 480
           ++YLKK+  DLC+ Y+ EAKWY++ Y+PTL+EY++ AW+SI GPVILVHAY   T+P T 
Sbjct: 424 IEYLKKSLVDLCKCYLQEAKWYYSGYQPTLQEYIEMAWLSIGGPVILVHAYFCFTNPITK 483

Query: 481 QDMESLVE-YADIIRWSSTILRLANDLGTSSDELERGDILKSIQCYMNDTGASESKAREH 540
           + M+   E Y +II+ S  I+RLA+D GT SDEL RGD+ KSIQCYM DTGASE +AREH
Sbjct: 484 ESMKFFTEGYPNIIQQSCLIVRLADDFGTFSDELNRGDVPKSIQCYMYDTGASEDEAREH 543

Query: 541 VKRLIDETWKKLNKAEVENSIFSQVFIERAKNLARMAQCVYQYGDGHGMGYQEINDRVMS 585
           +K LI ETWK +NK + +NS FS+ F+E  KNLAR A  +YQYGDGH        +R+ +
Sbjct: 544 IKFLICETWKDMNKNDEDNSCFSETFVEVCKNLARTALFMYQYGDGHASQNCLSKERIFA 603

BLAST of CmaCh20G010840 vs. ExPASy Swiss-Prot
Match: Q8L5K4 (Gamma-terpinene synthase, chloroplastic OS=Citrus limon OX=2708 PE=1 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 4.8e-172
Identity = 305/578 (52.77%), Postives = 416/578 (71.97%), Query Frame = 0

Query: 26  FVPPVLKVH-------GGVRMSSQTIVRRSGNYKPPIWKLEFIESLKSEFKGEMYVEHLN 85
           FVPP+ +V          ++   QTI+RRS +Y P IW  ++I+SL S++KGE Y   L 
Sbjct: 29  FVPPITQVQYPMAASTSSIKPVDQTIIRRSADYGPTIWSFDYIQSLDSKYKGESYARQLE 88

Query: 86  ELKEEIDIIM---NQIID-DPLKQLELIDTLQRLGISYHFENEIKDMLKRTYEKSYESDD 145
           +LKE++  ++   N+++D DPL QLELID L RLG+SYHFE+EIK  L R + K+     
Sbjct: 89  KLKEQVSAMLQQDNKVVDLDPLHQLELIDNLHRLGVSYHFEDEIKRTLDRIHNKN----- 148

Query: 146 WKKNNLHATSLEFRLLRQHGFNVS-QEVFNNFKDEMKNFSTRLYKD-LNGMLSLYEASFL 205
               +L+A +L+FR+LRQ+G+    +E F+ F DE  +F    + D   GML+LYEA++L
Sbjct: 149 -TNKSLYARALKFRILRQYGYKTPVKETFSRFMDEKGSFKLSSHSDECKGMLALYEAAYL 208

Query: 206 CIEGE-NILEAAKHFTINHLEKYMR-----SCKDEHEASIIRHALEFPLYWQISRLDTRW 265
            +E E +I   A  FT  +L++++         +E+  ++++HALE PL+W++ RL+ RW
Sbjct: 209 LVEEESSIFRDAIRFTTAYLKEWVAKHDIDKNDNEYLCTLVKHALELPLHWRMRRLEARW 268

Query: 266 FIDIYERKVDMNPILLEIAKLDFNQVQFIHQEDVKYASRWWRSTGFSEKFSFARDRLMEN 325
           FID+YE   DMNPILLE+AK+D+N VQ +HQED+KY SRWW+ TG  EK +FARDR++EN
Sbjct: 269 FIDVYESGPDMNPILLELAKVDYNIVQAVHQEDLKYVSRWWKKTGLGEKLNFARDRVVEN 328

Query: 326 FFWTVGVGFEPELSYFRRMATKVNALITTIDDIYDVYGTLDELELFTDAVERWDVGTMDQ 385
           FFWTVG  FEP+  Y RRM+  VN L+T+IDD+YDVYGTLDELELFTDAVERWD  T +Q
Sbjct: 329 FFWTVGDIFEPQFGYCRRMSAMVNCLLTSIDDVYDVYGTLDELELFTDAVERWDATTTEQ 388

Query: 386 LPDYMKICFFSLHNSINEMAFEALRDKGIN-VLKYLKKAWADLCQAYMLEAKWYHTSYKP 445
           LP YMK+CF +L+NS+NEM F ALRD+ +  ++ YLKKAWAD C++Y++EAKWY++ Y P
Sbjct: 389 LPYYMKLCFHALYNSVNEMGFIALRDQEVGMIIPYLKKAWADQCKSYLVEAKWYNSGYIP 448

Query: 446 TLEEYLDNAWISISGPVILVHAYAFVTSP-TLQDMESLVEYADIIRWSSTILRLANDLGT 505
           TL+EY++NAWIS++ PV+L+HAYAF  +P T + +E L +  DIIR SS I+RL +DLGT
Sbjct: 449 TLQEYMENAWISVTAPVMLLHAYAFTANPITKEALEFLQDSPDIIRISSMIVRLEDDLGT 508

Query: 506 SSDELERGDILKSIQCYMNDTGASESKAREHVKRLIDETWKKLNKAEVENSIF-SQVFIE 565
           SSDEL+RGD+ KSIQCYM++TG SE +AREH++ LI ETW K+N A   N  +   VFI 
Sbjct: 509 SSDELKRGDVPKSIQCYMHETGVSEDEAREHIRDLIAETWMKMNSARFGNPPYLPDVFIG 568

Query: 566 RAKNLARMAQCVYQYGDGHGMGYQEINDRVMSLLIRPI 582
            A NL RM+QC+Y YGDGHG+  +   DRV+SL I PI
Sbjct: 569 IAMNLVRMSQCMYLYGDGHGV-QENTKDRVLSLFIDPI 599

BLAST of CmaCh20G010840 vs. ExPASy TrEMBL
Match: A0A6J1I2T8 (terpene synthase 10-like OS=Cucurbita maxima OX=3661 GN=LOC111470415 PE=4 SV=1)

HSP 1 Score: 1196.4 bits (3094), Expect = 0.0e+00
Identity = 588/588 (100.00%), Postives = 588/588 (100.00%), Query Frame = 0

Query: 1   MALHQFFTSFTSLFIKRVSFDLNCSFVPPVLKVHGGVRMSSQTIVRRSGNYKPPIWKLEF 60
           MALHQFFTSFTSLFIKRVSFDLNCSFVPPVLKVHGGVRMSSQTIVRRSGNYKPPIWKLEF
Sbjct: 1   MALHQFFTSFTSLFIKRVSFDLNCSFVPPVLKVHGGVRMSSQTIVRRSGNYKPPIWKLEF 60

Query: 61  IESLKSEFKGEMYVEHLNELKEEIDIIMNQIIDDPLKQLELIDTLQRLGISYHFENEIKD 120
           IESLKSEFKGEMYVEHLNELKEEIDIIMNQIIDDPLKQLELIDTLQRLGISYHFENEIKD
Sbjct: 61  IESLKSEFKGEMYVEHLNELKEEIDIIMNQIIDDPLKQLELIDTLQRLGISYHFENEIKD 120

Query: 121 MLKRTYEKSYESDDWKKNNLHATSLEFRLLRQHGFNVSQEVFNNFKDEMKNFSTRLYKDL 180
           MLKRTYEKSYESDDWKKNNLHATSLEFRLLRQHGFNVSQEVFNNFKDEMKNFSTRLYKDL
Sbjct: 121 MLKRTYEKSYESDDWKKNNLHATSLEFRLLRQHGFNVSQEVFNNFKDEMKNFSTRLYKDL 180

Query: 181 NGMLSLYEASFLCIEGENILEAAKHFTINHLEKYMRSCKDEHEASIIRHALEFPLYWQIS 240
           NGMLSLYEASFLCIEGENILEAAKHFTINHLEKYMRSCKDEHEASIIRHALEFPLYWQIS
Sbjct: 181 NGMLSLYEASFLCIEGENILEAAKHFTINHLEKYMRSCKDEHEASIIRHALEFPLYWQIS 240

Query: 241 RLDTRWFIDIYERKVDMNPILLEIAKLDFNQVQFIHQEDVKYASRWWRSTGFSEKFSFAR 300
           RLDTRWFIDIYERKVDMNPILLEIAKLDFNQVQFIHQEDVKYASRWWRSTGFSEKFSFAR
Sbjct: 241 RLDTRWFIDIYERKVDMNPILLEIAKLDFNQVQFIHQEDVKYASRWWRSTGFSEKFSFAR 300

Query: 301 DRLMENFFWTVGVGFEPELSYFRRMATKVNALITTIDDIYDVYGTLDELELFTDAVERWD 360
           DRLMENFFWTVGVGFEPELSYFRRMATKVNALITTIDDIYDVYGTLDELELFTDAVERWD
Sbjct: 301 DRLMENFFWTVGVGFEPELSYFRRMATKVNALITTIDDIYDVYGTLDELELFTDAVERWD 360

Query: 361 VGTMDQLPDYMKICFFSLHNSINEMAFEALRDKGINVLKYLKKAWADLCQAYMLEAKWYH 420
           VGTMDQLPDYMKICFFSLHNSINEMAFEALRDKGINVLKYLKKAWADLCQAYMLEAKWYH
Sbjct: 361 VGTMDQLPDYMKICFFSLHNSINEMAFEALRDKGINVLKYLKKAWADLCQAYMLEAKWYH 420

Query: 421 TSYKPTLEEYLDNAWISISGPVILVHAYAFVTSPTLQDMESLVEYADIIRWSSTILRLAN 480
           TSYKPTLEEYLDNAWISISGPVILVHAYAFVTSPTLQDMESLVEYADIIRWSSTILRLAN
Sbjct: 421 TSYKPTLEEYLDNAWISISGPVILVHAYAFVTSPTLQDMESLVEYADIIRWSSTILRLAN 480

Query: 481 DLGTSSDELERGDILKSIQCYMNDTGASESKAREHVKRLIDETWKKLNKAEVENSIFSQV 540
           DLGTSSDELERGDILKSIQCYMNDTGASESKAREHVKRLIDETWKKLNKAEVENSIFSQV
Sbjct: 481 DLGTSSDELERGDILKSIQCYMNDTGASESKAREHVKRLIDETWKKLNKAEVENSIFSQV 540

Query: 541 FIERAKNLARMAQCVYQYGDGHGMGYQEINDRVMSLLIRPISTHSYNE 589
           FIERAKNLARMAQCVYQYGDGHGMGYQEINDRVMSLLIRPISTHSYNE
Sbjct: 541 FIERAKNLARMAQCVYQYGDGHGMGYQEINDRVMSLLIRPISTHSYNE 588

BLAST of CmaCh20G010840 vs. ExPASy TrEMBL
Match: A0A6J1EMY1 (terpene synthase 10-like OS=Cucurbita moschata OX=3662 GN=LOC111434992 PE=4 SV=1)

HSP 1 Score: 1105.1 bits (2857), Expect = 0.0e+00
Identity = 542/589 (92.02%), Postives = 566/589 (96.10%), Query Frame = 0

Query: 1   MALHQFFTSF-TSLFIKRVSFDLNCSFVPPVLKVHGGVRMSSQTIVRRSGNYKPPIWKLE 60
           M LHQ FTSF TSLFIKRVSFDLNCSFV P LKVHGGVRMSSQTIVRRSGNYKPPIWK E
Sbjct: 1   MTLHQPFTSFTTSLFIKRVSFDLNCSFVSPALKVHGGVRMSSQTIVRRSGNYKPPIWKAE 60

Query: 61  FIESLKSEFKGEMYVEHLNELKEEIDIIMNQIIDDPLKQLELIDTLQRLGISYHFENEIK 120
           FIESLKSEFKGE+YVE LNELKEEI IIMNQIIDDPLKQLELIDTLQRLGISYHFENEIK
Sbjct: 61  FIESLKSEFKGEIYVERLNELKEEIGIIMNQIIDDPLKQLELIDTLQRLGISYHFENEIK 120

Query: 121 DMLKRTYEKSYESDDWKKNNLHATSLEFRLLRQHGFNVSQEVFNNFKDEMKNFSTRLYKD 180
           D+LKRTYEK YE DDWKKNN++ATSLEFRLLRQHGF VSQEVFNN+KDEMKNFSTRLY+D
Sbjct: 121 DVLKRTYEKRYEGDDWKKNNMYATSLEFRLLRQHGFKVSQEVFNNYKDEMKNFSTRLYED 180

Query: 181 LNGMLSLYEASFLCIEGENILEAAKHFTINHLEKYMRSCKDEHEASIIRHALEFPLYWQI 240
           LNGMLSLYE SFL IEGENILEAAKHFTIN+L+KYMRSCKDEHEASIIRH LEFPL+W++
Sbjct: 181 LNGMLSLYEVSFLRIEGENILEAAKHFTINYLKKYMRSCKDEHEASIIRHVLEFPLHWRM 240

Query: 241 SRLDTRWFIDIYERKVDMNPILLEIAKLDFNQVQFIHQEDVKYASRWWRSTGFSEKFSFA 300
           SRLDTRWFID+YERKVDMNPILLEIAKLDFN+VQ IHQED+KYAS WWRSTGF EK SFA
Sbjct: 241 SRLDTRWFIDMYERKVDMNPILLEIAKLDFNRVQSIHQEDLKYASSWWRSTGFGEKLSFA 300

Query: 301 RDRLMENFFWTVGVGFEPELSYFRRMATKVNALITTIDDIYDVYGTLDELELFTDAVERW 360
           RDRLMENFFWTVGVGFEPELSYFRRMATKVNALITTIDDIYDVYGTLDELELFTDAVERW
Sbjct: 301 RDRLMENFFWTVGVGFEPELSYFRRMATKVNALITTIDDIYDVYGTLDELELFTDAVERW 360

Query: 361 DVGTMDQLPDYMKICFFSLHNSINEMAFEALRDKGINVLKYLKKAWADLCQAYMLEAKWY 420
           DVG +DQLPDYMKICFFSLHNS+NEMAFE LRDKGINVLKYLKKAWADLC+AYMLEAKWY
Sbjct: 361 DVGAVDQLPDYMKICFFSLHNSVNEMAFEVLRDKGINVLKYLKKAWADLCKAYMLEAKWY 420

Query: 421 HTSYKPTLEEYLDNAWISISGPVILVHAYAFVTSPTLQDMESLVEYADIIRWSSTILRLA 480
           HT+YKPTLEEYLDNAWISISGPVILVHAYAFVTSPTLQDMESLVEYADII WSSTILRLA
Sbjct: 421 HTNYKPTLEEYLDNAWISISGPVILVHAYAFVTSPTLQDMESLVEYADIIYWSSTILRLA 480

Query: 481 NDLGTSSDELERGDILKSIQCYMNDTGASESKAREHVKRLIDETWKKLNKAEVENSIFSQ 540
           +DLGTSSDELERGDI KSIQCYMNDTGASESKAREHV+RLIDETWKKLNKAEVENSIF Q
Sbjct: 481 DDLGTSSDELERGDIPKSIQCYMNDTGASESKAREHVRRLIDETWKKLNKAEVENSIFPQ 540

Query: 541 VFIERAKNLARMAQCVYQYGDGHGMGYQEINDRVMSLLIRPISTHSYNE 589
           VFIERAKNLARMAQC+YQ+GDGHGMGYQEINDRVMSLLIRPISTHSY++
Sbjct: 541 VFIERAKNLARMAQCMYQHGDGHGMGYQEINDRVMSLLIRPISTHSYSK 589

BLAST of CmaCh20G010840 vs. ExPASy TrEMBL
Match: A0A6J1EJW9 (terpene synthase 10-like OS=Cucurbita moschata OX=3662 GN=LOC111434993 PE=4 SV=1)

HSP 1 Score: 1007.3 bits (2603), Expect = 2.7e-290
Identity = 478/588 (81.29%), Postives = 539/588 (91.67%), Query Frame = 0

Query: 1   MALHQFFTSFTS-LFIKRVSFDLNCSFVPPVLKVHGGVRMSSQTIVRRSGNYKPPIWKLE 60
           MALH F TSFT+  F+KRV FDLN SF+PP +KV+    +S+QTIVRRSGNY+PPIWK E
Sbjct: 1   MALHPFLTSFTTDFFMKRVYFDLNSSFIPPTVKVNCVAIVSNQTIVRRSGNYQPPIWKHE 60

Query: 61  FIESLKSEFKGEMYVEHLNELKEEIDIIMNQIIDDPLKQLELIDTLQRLGISYHFENEIK 120
           +I+SL SEFKGE+YVE  NELK+E+D+++NQIIDDPLK  ELIDTLQRLGISYHFENEIK
Sbjct: 61  YIQSLNSEFKGEIYVERFNELKKEVDVMINQIIDDPLKNFELIDTLQRLGISYHFENEIK 120

Query: 121 DMLKRTYEKSYESDDWKKNNLHATSLEFRLLRQHGFNVSQEVFNNFKDEMKNFSTRLYKD 180
           D+LKRTYEKSYESDDWKKNNL+ATSLEFR+LRQHG NVSQEVFNNFKDE KNFS +LY+D
Sbjct: 121 DLLKRTYEKSYESDDWKKNNLYATSLEFRILRQHGLNVSQEVFNNFKDETKNFSKQLYED 180

Query: 181 LNGMLSLYEASFLCIEGENILEAAKHFTINHLEKYMRSCKDEHEASIIRHALEFPLYWQI 240
           LNG+LSLYEASFLCIEGENILE AKHFTIN+LE YMRSCKDE+EASIIRHALE P++W++
Sbjct: 181 LNGILSLYEASFLCIEGENILETAKHFTINYLENYMRSCKDEYEASIIRHALELPIHWRL 240

Query: 241 SRLDTRWFIDIYERKVDMNPILLEIAKLDFNQVQFIHQEDVKYASRWWRSTGFSEKFSFA 300
           SRLDTRWFIDIYERK DMNPILLE+AKLDFN+VQ IHQED+KYAS WWRSTGF +K SFA
Sbjct: 241 SRLDTRWFIDIYERKEDMNPILLELAKLDFNRVQSIHQEDLKYASSWWRSTGFGKKLSFA 300

Query: 301 RDRLMENFFWTVGVGFEPELSYFRRMATKVNALITTIDDIYDVYGTLDELELFTDAVERW 360
           RDRLMENFFWTVGVGFEPELSYFRRM  K+NAL T+IDD+YD+YGTLDELELFTDAVERW
Sbjct: 301 RDRLMENFFWTVGVGFEPELSYFRRMGVKINALTTSIDDVYDIYGTLDELELFTDAVERW 360

Query: 361 DVGTMDQLPDYMKICFFSLHNSINEMAFEALRDKGINVLKYLKKAWADLCQAYMLEAKWY 420
           DVG +DQLPDYMKICFF LHN+INEMAF+ALRDKGINV++YLKK W DLC+AYMLEA+WY
Sbjct: 361 DVGAVDQLPDYMKICFFVLHNAINEMAFDALRDKGINVIQYLKKLWVDLCKAYMLEARWY 420

Query: 421 HTSYKPTLEEYLDNAWISISGPVILVHAYAFVTSPTLQDMESLVEYADIIRWSSTILRLA 480
           H+ YKPTLEEYLDNAWISISGPVIL+HAY FVTSPT QDMESLVEYADIIRWSSTI+RLA
Sbjct: 421 HSRYKPTLEEYLDNAWISISGPVILIHAYVFVTSPTFQDMESLVEYADIIRWSSTIVRLA 480

Query: 481 NDLGTSSDELERGDILKSIQCYMNDTGASESKAREHVKRLIDETWKKLNKAEVENSIFSQ 540
           +DLGTSSDE++RGDI KS+QCYMNDTGASESKAREHV+ LIDETWKKLNK +VENS+F  
Sbjct: 481 DDLGTSSDEIKRGDIPKSVQCYMNDTGASESKAREHVRHLIDETWKKLNKIDVENSVFPP 540

Query: 541 VFIERAKNLARMAQCVYQYGDGHGMGYQEINDRVMSLLIRPISTHSYN 588
           VFI+ AKNLARMAQC+YQ+GDGHG+G+QE N+ + SLLIRPIS  SY+
Sbjct: 541 VFIKIAKNLARMAQCIYQHGDGHGLGHQETNEDIASLLIRPISIRSYS 588

BLAST of CmaCh20G010840 vs. ExPASy TrEMBL
Match: A0A6J1EJ26 (terpene synthase 10-like OS=Cucurbita moschata OX=3662 GN=LOC111434910 PE=4 SV=1)

HSP 1 Score: 944.9 bits (2441), Expect = 1.6e-271
Identity = 455/589 (77.25%), Postives = 522/589 (88.62%), Query Frame = 0

Query: 1   MALHQFFTSF-TSLFIKRVSFDLNCSFVPPVLKVHGGVRMSSQTIVRRSGNYKPPIWKLE 60
           MAL++F T F  +  +KRV FD N SF+ P +K +   R+S+Q IVRRSGNYKPP+WK E
Sbjct: 1   MALYKFLTPFMMNPSLKRVHFDQNSSFLCPAVKANSVARVSNQIIVRRSGNYKPPVWKHE 60

Query: 61  FIESLKSEFKGEMYVEHLNELKEEIDIIMNQIIDDPLKQLELIDTLQRLGISYHFENEIK 120
           +I+SL SEFKGE+YVE  NELKEE+D+++NQIIDDPLK LELIDTLQRLGISY FENEIK
Sbjct: 61  YIQSLNSEFKGEIYVERFNELKEEVDVMINQIIDDPLKNLELIDTLQRLGISYLFENEIK 120

Query: 121 DMLKRTYEKSYESDDWKKNNLHATSLEFRLLRQHGFNVSQEVFNNFKDEMKNFSTRLYKD 180
           D+LKRTYEKSYESDDWKKNNL+ATSLEFRLLRQHGFNVSQEVFNNF DE KNF+T LY+D
Sbjct: 121 DLLKRTYEKSYESDDWKKNNLYATSLEFRLLRQHGFNVSQEVFNNFMDETKNFNTGLYQD 180

Query: 181 LNGMLSLYEASFLCIEGENILEAAKHFTINHLEKYMRSCKDEHEASIIRHALEFPLYWQI 240
           LNGMLSLYEASFLCIEGENILEAAK+FTIN+LEKYMRSC+DE+EASIIRHALE PL+W+I
Sbjct: 181 LNGMLSLYEASFLCIEGENILEAAKYFTINYLEKYMRSCQDEYEASIIRHALELPLHWRI 240

Query: 241 SRLDTRWFIDIYERKVDMNPILLEIAKLDFNQVQFIHQEDVKYASRWWRSTGFSEKFSFA 300
           SRL+ RWFIDIYERKV+MN ILLEIAKLDFN+VQ +HQED+KYASRWWR+TGFSEK SFA
Sbjct: 241 SRLEARWFIDIYERKVNMNSILLEIAKLDFNRVQSVHQEDLKYASRWWRNTGFSEKLSFA 300

Query: 301 RDRLMENFFWTVGVGFEPELSYFRRMATKVNALITTIDDIYDVYGTLDELELFTDAVERW 360
           RDRLMENFFWTVG GFEPELSYFRRM+TK+ ALITTIDD+YDVYGTLDELELFT AVERW
Sbjct: 301 RDRLMENFFWTVGFGFEPELSYFRRMSTKIIALITTIDDVYDVYGTLDELELFTKAVERW 360

Query: 361 DVGTMDQLPDYMKICFFSLHNSINEMAFEALRDKGINVLKYLKKAWADLCQAYMLEAKWY 420
           +V  MD+LPDYMKICF  LHNSINEM F+ LRDK INV++Y KK W DLC++Y+LEAK +
Sbjct: 361 NVDAMDKLPDYMKICFLGLHNSINEMTFDVLRDKEINVIQYFKKTWIDLCKSYLLEAKCF 420

Query: 421 HTSYKPTLEEYLDNAWISISGPVILVHAYAFVTSPTLQDMESLVEYADIIRWSSTILRLA 480
           H+ YKPTLEEYLDNAWIS+ GPV+LVHAY FVTSP L+DMESL E+ DIIR SSTI+RLA
Sbjct: 421 HSGYKPTLEEYLDNAWISVGGPVVLVHAYVFVTSPMLKDMESLEEHIDIIRCSSTIVRLA 480

Query: 481 NDLGTSSDELERGDILKSIQCYMNDTGASESKAREHVKRLIDETWKKLNKAEVENSIFSQ 540
           +DL TS+DELERGD+ KSIQCYMNDTGASE+ AREH+K LIDETWKKLN  EVENSIF Q
Sbjct: 481 DDLATSTDELERGDVPKSIQCYMNDTGASENNAREHIKHLIDETWKKLNMVEVENSIFLQ 540

Query: 541 VFIERAKNLARMAQCVYQYGDGHGMGYQEINDRVMSLLIRPISTHSYNE 589
           V I+ A+NLAR AQC++Q+GDGHG+G +E N  VMSL++RPIS H Y++
Sbjct: 541 VLIKIAQNLARTAQCMFQFGDGHGIGNKETNANVMSLIVRPISIHPYSK 589

BLAST of CmaCh20G010840 vs. ExPASy TrEMBL
Match: A0A6J1I436 (terpene synthase 10-like OS=Cucurbita maxima OX=3661 GN=LOC111470418 PE=4 SV=1)

HSP 1 Score: 928.3 bits (2398), Expect = 1.6e-266
Identity = 456/583 (78.22%), Postives = 511/583 (87.65%), Query Frame = 0

Query: 1   MALHQFFTSFT-SLFIKRVSFDLNCSFVPPVLKVHGGVRMSSQTIVRRSGNYKPPIWKLE 60
           MAL++F T FT +  IKRV FD N S + P  KV    ++S+Q I RRSGNYKPPIWK E
Sbjct: 1   MALYKFLTPFTINPSIKRVYFDQNSSIICPTAKVISDAKVSNQNITRRSGNYKPPIWKYE 60

Query: 61  FIESLKSEFKGEMYVEHLNELKEEIDIIMNQIIDDPLKQLELIDTLQRLGISYHFENEIK 120
           +I++L SEFK E+YVE  NELKE+I ++MNQI DDPLK+L+LIDTLQRLGISYHFENEIK
Sbjct: 61  YIQALNSEFKEELYVERFNELKEKIGVLMNQIKDDPLKRLKLIDTLQRLGISYHFENEIK 120

Query: 121 DMLKRTYEKSYESDDWKKNNLHATSLEFRLLRQHGFNVSQEVFNNFKDEMKNFSTRLYKD 180
           D+LKR YEK ++SDD +KNNL+A SLEFRLLRQHGF+VSQ+VFNNF +E KNFST LY+D
Sbjct: 121 DVLKRIYEKRFKSDDSEKNNLYAISLEFRLLRQHGFDVSQDVFNNF-EETKNFSTGLYQD 180

Query: 181 LNGMLSLYEASFLCIEGENILEAAKHFTINHLEKYMRSCKDEHEASIIRHALEFPLYWQI 240
           LNGM+SLYEASFLC EGENILEAAKHFTIN+LEKY RSCKDE+EASIIRHALE PL+W+I
Sbjct: 181 LNGMVSLYEASFLCKEGENILEAAKHFTINYLEKYTRSCKDEYEASIIRHALELPLHWRI 240

Query: 241 SRLDTRWFIDIYERKVDMNPILLEIAKLDFNQVQFIHQEDVKYASRWWRSTGFSEKFSFA 300
           SRL+ RWFIDIY+RKVDMNPILLEIAKLDFN+VQ IHQED+KYASRWW S GFSEK SFA
Sbjct: 241 SRLEARWFIDIYQRKVDMNPILLEIAKLDFNRVQSIHQEDLKYASRWWSSIGFSEKLSFA 300

Query: 301 RDRLMENFFWTVGVGFEPELSYFRRMATKVNALITTIDDIYDVYGTLDELELFTDAVERW 360
           RDRLME FFWTVG GFEP+LSYFRRM TKVNALITTIDD+YDVYGTLDELELFTDA+ERW
Sbjct: 301 RDRLMECFFWTVGFGFEPQLSYFRRMVTKVNALITTIDDVYDVYGTLDELELFTDAIERW 360

Query: 361 DVGTMDQLPDYMKICFFSLHNSINEMAFEALRDKGINVLKYLKKAWADLCQAYMLEAKWY 420
           +V  MDQLPDYMKICFF L+NSINEMAF+ALRDKG NV+  LKK W DLC++YM EAKWY
Sbjct: 361 NVDAMDQLPDYMKICFFGLYNSINEMAFDALRDKGTNVIHDLKKTWVDLCKSYMFEAKWY 420

Query: 421 HTSYKPTLEEYLDNAWISISGPVILVHAYAFVTSPTLQDMESLVEYADIIRWSSTILRLA 480
           HT YKPTLEEYLDNAWISI GPVILVHAY FVTSP LQDMESL  + DIIR SS ILRLA
Sbjct: 421 HTDYKPTLEEYLDNAWISIGGPVILVHAYVFVTSPMLQDMESLEAHIDIIRCSSIILRLA 480

Query: 481 NDLGTSSDELERGDILKSIQCYMNDTGASESKAREHVKRLIDETWKKLNKAEVENSIFSQ 540
           +DLGTS++ELERGD+ KSIQCYMNDTGASE+ AREH+K LIDETWKKLN AEV+NSI   
Sbjct: 481 DDLGTSTNELERGDVPKSIQCYMNDTGASENNAREHIKNLIDETWKKLNTAEVKNSILPH 540

Query: 541 VFIERAKNLARMAQCVYQYGDGHGMGYQEINDRVMSLLIRPIS 583
           VFI+ AKNLARM QC+YQYGDGHG+G QEIND VMSLLI+PIS
Sbjct: 541 VFIQIAKNLARMGQCMYQYGDGHGIGRQEINDGVMSLLIQPIS 582

BLAST of CmaCh20G010840 vs. NCBI nr
Match: XP_022971747.1 (terpene synthase 10-like [Cucurbita maxima])

HSP 1 Score: 1196.4 bits (3094), Expect = 0.0e+00
Identity = 588/588 (100.00%), Postives = 588/588 (100.00%), Query Frame = 0

Query: 1   MALHQFFTSFTSLFIKRVSFDLNCSFVPPVLKVHGGVRMSSQTIVRRSGNYKPPIWKLEF 60
           MALHQFFTSFTSLFIKRVSFDLNCSFVPPVLKVHGGVRMSSQTIVRRSGNYKPPIWKLEF
Sbjct: 1   MALHQFFTSFTSLFIKRVSFDLNCSFVPPVLKVHGGVRMSSQTIVRRSGNYKPPIWKLEF 60

Query: 61  IESLKSEFKGEMYVEHLNELKEEIDIIMNQIIDDPLKQLELIDTLQRLGISYHFENEIKD 120
           IESLKSEFKGEMYVEHLNELKEEIDIIMNQIIDDPLKQLELIDTLQRLGISYHFENEIKD
Sbjct: 61  IESLKSEFKGEMYVEHLNELKEEIDIIMNQIIDDPLKQLELIDTLQRLGISYHFENEIKD 120

Query: 121 MLKRTYEKSYESDDWKKNNLHATSLEFRLLRQHGFNVSQEVFNNFKDEMKNFSTRLYKDL 180
           MLKRTYEKSYESDDWKKNNLHATSLEFRLLRQHGFNVSQEVFNNFKDEMKNFSTRLYKDL
Sbjct: 121 MLKRTYEKSYESDDWKKNNLHATSLEFRLLRQHGFNVSQEVFNNFKDEMKNFSTRLYKDL 180

Query: 181 NGMLSLYEASFLCIEGENILEAAKHFTINHLEKYMRSCKDEHEASIIRHALEFPLYWQIS 240
           NGMLSLYEASFLCIEGENILEAAKHFTINHLEKYMRSCKDEHEASIIRHALEFPLYWQIS
Sbjct: 181 NGMLSLYEASFLCIEGENILEAAKHFTINHLEKYMRSCKDEHEASIIRHALEFPLYWQIS 240

Query: 241 RLDTRWFIDIYERKVDMNPILLEIAKLDFNQVQFIHQEDVKYASRWWRSTGFSEKFSFAR 300
           RLDTRWFIDIYERKVDMNPILLEIAKLDFNQVQFIHQEDVKYASRWWRSTGFSEKFSFAR
Sbjct: 241 RLDTRWFIDIYERKVDMNPILLEIAKLDFNQVQFIHQEDVKYASRWWRSTGFSEKFSFAR 300

Query: 301 DRLMENFFWTVGVGFEPELSYFRRMATKVNALITTIDDIYDVYGTLDELELFTDAVERWD 360
           DRLMENFFWTVGVGFEPELSYFRRMATKVNALITTIDDIYDVYGTLDELELFTDAVERWD
Sbjct: 301 DRLMENFFWTVGVGFEPELSYFRRMATKVNALITTIDDIYDVYGTLDELELFTDAVERWD 360

Query: 361 VGTMDQLPDYMKICFFSLHNSINEMAFEALRDKGINVLKYLKKAWADLCQAYMLEAKWYH 420
           VGTMDQLPDYMKICFFSLHNSINEMAFEALRDKGINVLKYLKKAWADLCQAYMLEAKWYH
Sbjct: 361 VGTMDQLPDYMKICFFSLHNSINEMAFEALRDKGINVLKYLKKAWADLCQAYMLEAKWYH 420

Query: 421 TSYKPTLEEYLDNAWISISGPVILVHAYAFVTSPTLQDMESLVEYADIIRWSSTILRLAN 480
           TSYKPTLEEYLDNAWISISGPVILVHAYAFVTSPTLQDMESLVEYADIIRWSSTILRLAN
Sbjct: 421 TSYKPTLEEYLDNAWISISGPVILVHAYAFVTSPTLQDMESLVEYADIIRWSSTILRLAN 480

Query: 481 DLGTSSDELERGDILKSIQCYMNDTGASESKAREHVKRLIDETWKKLNKAEVENSIFSQV 540
           DLGTSSDELERGDILKSIQCYMNDTGASESKAREHVKRLIDETWKKLNKAEVENSIFSQV
Sbjct: 481 DLGTSSDELERGDILKSIQCYMNDTGASESKAREHVKRLIDETWKKLNKAEVENSIFSQV 540

Query: 541 FIERAKNLARMAQCVYQYGDGHGMGYQEINDRVMSLLIRPISTHSYNE 589
           FIERAKNLARMAQCVYQYGDGHGMGYQEINDRVMSLLIRPISTHSYNE
Sbjct: 541 FIERAKNLARMAQCVYQYGDGHGMGYQEINDRVMSLLIRPISTHSYNE 588

BLAST of CmaCh20G010840 vs. NCBI nr
Match: XP_023512342.1 (terpene synthase 10-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1111.7 bits (2874), Expect = 0.0e+00
Identity = 544/589 (92.36%), Postives = 566/589 (96.10%), Query Frame = 0

Query: 1   MALHQFFTSF-TSLFIKRVSFDLNCSFVPPVLKVHGGVRMSSQTIVRRSGNYKPPIWKLE 60
           MALHQ FTSF TSLFIKRVSFDLNCSFVPP LKVHG VRMSSQTIVRRSGNYKPPIWK E
Sbjct: 1   MALHQSFTSFTTSLFIKRVSFDLNCSFVPPALKVHGSVRMSSQTIVRRSGNYKPPIWKPE 60

Query: 61  FIESLKSEFKGEMYVEHLNELKEEIDIIMNQIIDDPLKQLELIDTLQRLGISYHFENEIK 120
           FIESLKSEFKGEMYVE LNELKEEI IIM+QIIDDPLKQLELID LQRLGISYHFENEIK
Sbjct: 61  FIESLKSEFKGEMYVERLNELKEEIGIIMSQIIDDPLKQLELIDILQRLGISYHFENEIK 120

Query: 121 DMLKRTYEKSYESDDWKKNNLHATSLEFRLLRQHGFNVSQEVFNNFKDEMKNFSTRLYKD 180
           D+LKRTYEK YE DDWKKNNL+ATSLEFRLLRQHGF VSQEVFNN+ DEMKNFSTRLY+D
Sbjct: 121 DVLKRTYEKRYEGDDWKKNNLYATSLEFRLLRQHGFKVSQEVFNNYNDEMKNFSTRLYED 180

Query: 181 LNGMLSLYEASFLCIEGENILEAAKHFTINHLEKYMRSCKDEHEASIIRHALEFPLYWQI 240
           LNGMLSLYEASFLCIEGENILE AKHFTIN+L+KYMRSCKDEHEASIIRHALEFPL+W++
Sbjct: 181 LNGMLSLYEASFLCIEGENILETAKHFTINYLKKYMRSCKDEHEASIIRHALEFPLHWRM 240

Query: 241 SRLDTRWFIDIYERKVDMNPILLEIAKLDFNQVQFIHQEDVKYASRWWRSTGFSEKFSFA 300
           SRLDTRWF+DIYERKVDMNPILLEIAKLDFN+VQ IHQED+KYAS WWR TGF +K SFA
Sbjct: 241 SRLDTRWFVDIYERKVDMNPILLEIAKLDFNRVQSIHQEDLKYASSWWRRTGFGQKLSFA 300

Query: 301 RDRLMENFFWTVGVGFEPELSYFRRMATKVNALITTIDDIYDVYGTLDELELFTDAVERW 360
           RDRLMENFFWTVGVGFEPELSYFRRMATKVNAL+TTIDDIYD YGTLDELELFTDAVERW
Sbjct: 301 RDRLMENFFWTVGVGFEPELSYFRRMATKVNALVTTIDDIYDFYGTLDELELFTDAVERW 360

Query: 361 DVGTMDQLPDYMKICFFSLHNSINEMAFEALRDKGINVLKYLKKAWADLCQAYMLEAKWY 420
           DVG +DQLPDYMKICFFSLHNSINEMAFEALRDKGINVLKYLKKAWADLC+AYMLEAKWY
Sbjct: 361 DVGAVDQLPDYMKICFFSLHNSINEMAFEALRDKGINVLKYLKKAWADLCKAYMLEAKWY 420

Query: 421 HTSYKPTLEEYLDNAWISISGPVILVHAYAFVTSPTLQDMESLVEYADIIRWSSTILRLA 480
           HTSYKP LEEYLDNAWISISGPVILVHAYAFVTSPTLQDMESLVEYADIIRWSSTILRLA
Sbjct: 421 HTSYKPMLEEYLDNAWISISGPVILVHAYAFVTSPTLQDMESLVEYADIIRWSSTILRLA 480

Query: 481 NDLGTSSDELERGDILKSIQCYMNDTGASESKAREHVKRLIDETWKKLNKAEVENSIFSQ 540
           NDLGTSSDELERGDI KSIQCYMNDTGASESKAREHV+RLIDETWKKLNKAEVENSIF Q
Sbjct: 481 NDLGTSSDELERGDIPKSIQCYMNDTGASESKAREHVRRLIDETWKKLNKAEVENSIFPQ 540

Query: 541 VFIERAKNLARMAQCVYQYGDGHGMGYQEINDRVMSLLIRPISTHSYNE 589
           +FIERAKNLARMAQC+YQYGDGHGMGYQEINDRVMSL+IRPISTHSY+E
Sbjct: 541 LFIERAKNLARMAQCMYQYGDGHGMGYQEINDRVMSLVIRPISTHSYSE 589

BLAST of CmaCh20G010840 vs. NCBI nr
Match: XP_022928103.1 (terpene synthase 10-like [Cucurbita moschata])

HSP 1 Score: 1105.1 bits (2857), Expect = 0.0e+00
Identity = 542/589 (92.02%), Postives = 566/589 (96.10%), Query Frame = 0

Query: 1   MALHQFFTSF-TSLFIKRVSFDLNCSFVPPVLKVHGGVRMSSQTIVRRSGNYKPPIWKLE 60
           M LHQ FTSF TSLFIKRVSFDLNCSFV P LKVHGGVRMSSQTIVRRSGNYKPPIWK E
Sbjct: 1   MTLHQPFTSFTTSLFIKRVSFDLNCSFVSPALKVHGGVRMSSQTIVRRSGNYKPPIWKAE 60

Query: 61  FIESLKSEFKGEMYVEHLNELKEEIDIIMNQIIDDPLKQLELIDTLQRLGISYHFENEIK 120
           FIESLKSEFKGE+YVE LNELKEEI IIMNQIIDDPLKQLELIDTLQRLGISYHFENEIK
Sbjct: 61  FIESLKSEFKGEIYVERLNELKEEIGIIMNQIIDDPLKQLELIDTLQRLGISYHFENEIK 120

Query: 121 DMLKRTYEKSYESDDWKKNNLHATSLEFRLLRQHGFNVSQEVFNNFKDEMKNFSTRLYKD 180
           D+LKRTYEK YE DDWKKNN++ATSLEFRLLRQHGF VSQEVFNN+KDEMKNFSTRLY+D
Sbjct: 121 DVLKRTYEKRYEGDDWKKNNMYATSLEFRLLRQHGFKVSQEVFNNYKDEMKNFSTRLYED 180

Query: 181 LNGMLSLYEASFLCIEGENILEAAKHFTINHLEKYMRSCKDEHEASIIRHALEFPLYWQI 240
           LNGMLSLYE SFL IEGENILEAAKHFTIN+L+KYMRSCKDEHEASIIRH LEFPL+W++
Sbjct: 181 LNGMLSLYEVSFLRIEGENILEAAKHFTINYLKKYMRSCKDEHEASIIRHVLEFPLHWRM 240

Query: 241 SRLDTRWFIDIYERKVDMNPILLEIAKLDFNQVQFIHQEDVKYASRWWRSTGFSEKFSFA 300
           SRLDTRWFID+YERKVDMNPILLEIAKLDFN+VQ IHQED+KYAS WWRSTGF EK SFA
Sbjct: 241 SRLDTRWFIDMYERKVDMNPILLEIAKLDFNRVQSIHQEDLKYASSWWRSTGFGEKLSFA 300

Query: 301 RDRLMENFFWTVGVGFEPELSYFRRMATKVNALITTIDDIYDVYGTLDELELFTDAVERW 360
           RDRLMENFFWTVGVGFEPELSYFRRMATKVNALITTIDDIYDVYGTLDELELFTDAVERW
Sbjct: 301 RDRLMENFFWTVGVGFEPELSYFRRMATKVNALITTIDDIYDVYGTLDELELFTDAVERW 360

Query: 361 DVGTMDQLPDYMKICFFSLHNSINEMAFEALRDKGINVLKYLKKAWADLCQAYMLEAKWY 420
           DVG +DQLPDYMKICFFSLHNS+NEMAFE LRDKGINVLKYLKKAWADLC+AYMLEAKWY
Sbjct: 361 DVGAVDQLPDYMKICFFSLHNSVNEMAFEVLRDKGINVLKYLKKAWADLCKAYMLEAKWY 420

Query: 421 HTSYKPTLEEYLDNAWISISGPVILVHAYAFVTSPTLQDMESLVEYADIIRWSSTILRLA 480
           HT+YKPTLEEYLDNAWISISGPVILVHAYAFVTSPTLQDMESLVEYADII WSSTILRLA
Sbjct: 421 HTNYKPTLEEYLDNAWISISGPVILVHAYAFVTSPTLQDMESLVEYADIIYWSSTILRLA 480

Query: 481 NDLGTSSDELERGDILKSIQCYMNDTGASESKAREHVKRLIDETWKKLNKAEVENSIFSQ 540
           +DLGTSSDELERGDI KSIQCYMNDTGASESKAREHV+RLIDETWKKLNKAEVENSIF Q
Sbjct: 481 DDLGTSSDELERGDIPKSIQCYMNDTGASESKAREHVRRLIDETWKKLNKAEVENSIFPQ 540

Query: 541 VFIERAKNLARMAQCVYQYGDGHGMGYQEINDRVMSLLIRPISTHSYNE 589
           VFIERAKNLARMAQC+YQ+GDGHGMGYQEINDRVMSLLIRPISTHSY++
Sbjct: 541 VFIERAKNLARMAQCMYQHGDGHGMGYQEINDRVMSLLIRPISTHSYSK 589

BLAST of CmaCh20G010840 vs. NCBI nr
Match: XP_022928104.1 (terpene synthase 10-like [Cucurbita moschata])

HSP 1 Score: 1007.3 bits (2603), Expect = 5.5e-290
Identity = 478/588 (81.29%), Postives = 539/588 (91.67%), Query Frame = 0

Query: 1   MALHQFFTSFTS-LFIKRVSFDLNCSFVPPVLKVHGGVRMSSQTIVRRSGNYKPPIWKLE 60
           MALH F TSFT+  F+KRV FDLN SF+PP +KV+    +S+QTIVRRSGNY+PPIWK E
Sbjct: 1   MALHPFLTSFTTDFFMKRVYFDLNSSFIPPTVKVNCVAIVSNQTIVRRSGNYQPPIWKHE 60

Query: 61  FIESLKSEFKGEMYVEHLNELKEEIDIIMNQIIDDPLKQLELIDTLQRLGISYHFENEIK 120
           +I+SL SEFKGE+YVE  NELK+E+D+++NQIIDDPLK  ELIDTLQRLGISYHFENEIK
Sbjct: 61  YIQSLNSEFKGEIYVERFNELKKEVDVMINQIIDDPLKNFELIDTLQRLGISYHFENEIK 120

Query: 121 DMLKRTYEKSYESDDWKKNNLHATSLEFRLLRQHGFNVSQEVFNNFKDEMKNFSTRLYKD 180
           D+LKRTYEKSYESDDWKKNNL+ATSLEFR+LRQHG NVSQEVFNNFKDE KNFS +LY+D
Sbjct: 121 DLLKRTYEKSYESDDWKKNNLYATSLEFRILRQHGLNVSQEVFNNFKDETKNFSKQLYED 180

Query: 181 LNGMLSLYEASFLCIEGENILEAAKHFTINHLEKYMRSCKDEHEASIIRHALEFPLYWQI 240
           LNG+LSLYEASFLCIEGENILE AKHFTIN+LE YMRSCKDE+EASIIRHALE P++W++
Sbjct: 181 LNGILSLYEASFLCIEGENILETAKHFTINYLENYMRSCKDEYEASIIRHALELPIHWRL 240

Query: 241 SRLDTRWFIDIYERKVDMNPILLEIAKLDFNQVQFIHQEDVKYASRWWRSTGFSEKFSFA 300
           SRLDTRWFIDIYERK DMNPILLE+AKLDFN+VQ IHQED+KYAS WWRSTGF +K SFA
Sbjct: 241 SRLDTRWFIDIYERKEDMNPILLELAKLDFNRVQSIHQEDLKYASSWWRSTGFGKKLSFA 300

Query: 301 RDRLMENFFWTVGVGFEPELSYFRRMATKVNALITTIDDIYDVYGTLDELELFTDAVERW 360
           RDRLMENFFWTVGVGFEPELSYFRRM  K+NAL T+IDD+YD+YGTLDELELFTDAVERW
Sbjct: 301 RDRLMENFFWTVGVGFEPELSYFRRMGVKINALTTSIDDVYDIYGTLDELELFTDAVERW 360

Query: 361 DVGTMDQLPDYMKICFFSLHNSINEMAFEALRDKGINVLKYLKKAWADLCQAYMLEAKWY 420
           DVG +DQLPDYMKICFF LHN+INEMAF+ALRDKGINV++YLKK W DLC+AYMLEA+WY
Sbjct: 361 DVGAVDQLPDYMKICFFVLHNAINEMAFDALRDKGINVIQYLKKLWVDLCKAYMLEARWY 420

Query: 421 HTSYKPTLEEYLDNAWISISGPVILVHAYAFVTSPTLQDMESLVEYADIIRWSSTILRLA 480
           H+ YKPTLEEYLDNAWISISGPVIL+HAY FVTSPT QDMESLVEYADIIRWSSTI+RLA
Sbjct: 421 HSRYKPTLEEYLDNAWISISGPVILIHAYVFVTSPTFQDMESLVEYADIIRWSSTIVRLA 480

Query: 481 NDLGTSSDELERGDILKSIQCYMNDTGASESKAREHVKRLIDETWKKLNKAEVENSIFSQ 540
           +DLGTSSDE++RGDI KS+QCYMNDTGASESKAREHV+ LIDETWKKLNK +VENS+F  
Sbjct: 481 DDLGTSSDEIKRGDIPKSVQCYMNDTGASESKAREHVRHLIDETWKKLNKIDVENSVFPP 540

Query: 541 VFIERAKNLARMAQCVYQYGDGHGMGYQEINDRVMSLLIRPISTHSYN 588
           VFI+ AKNLARMAQC+YQ+GDGHG+G+QE N+ + SLLIRPIS  SY+
Sbjct: 541 VFIKIAKNLARMAQCIYQHGDGHGLGHQETNEDIASLLIRPISIRSYS 588

BLAST of CmaCh20G010840 vs. NCBI nr
Match: XP_023513213.1 (terpene synthase 10-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 993.8 bits (2568), Expect = 6.3e-286
Identity = 473/588 (80.44%), Postives = 536/588 (91.16%), Query Frame = 0

Query: 1   MALHQFFTSFTS-LFIKRVSFDLNCSFVPPVLKVHGGVRMSSQTIVRRSGNYKPPIWKLE 60
           MALH F TSFT+  F+KRV FDLN SF+P  +KV+    +S+QTI+RRSGNY+PPIWK E
Sbjct: 1   MALHPFLTSFTTDHFMKRVYFDLNSSFIPLTVKVNCVAIVSNQTIIRRSGNYQPPIWKHE 60

Query: 61  FIESLKSEFKGEMYVEHLNELKEEIDIIMNQIIDDPLKQLELIDTLQRLGISYHFENEIK 120
           +I+SL SEFKGE+YVE  NELK+E+D+++NQIIDDPLK LELIDTLQRLGISYHFENEI 
Sbjct: 61  YIQSLNSEFKGEIYVERFNELKKEVDVMINQIIDDPLKNLELIDTLQRLGISYHFENEIN 120

Query: 121 DMLKRTYEKSYESDDWKKNNLHATSLEFRLLRQHGFNVSQEVFNNFKDEMKNFSTRLYKD 180
           D+LKRTYEKSYESDDWKKNNL+ATSLEFR+LRQHG +VSQEVFNNFKDE K FS +LY+D
Sbjct: 121 DLLKRTYEKSYESDDWKKNNLYATSLEFRILRQHGLSVSQEVFNNFKDETKFFSKQLYED 180

Query: 181 LNGMLSLYEASFLCIEGENILEAAKHFTINHLEKYMRSCKDEHEASIIRHALEFPLYWQI 240
           LNG+LSLYEASFLC EGENILE AKHFTIN+LE YMRSCKDE+EASIIRHALE P++W++
Sbjct: 181 LNGILSLYEASFLCTEGENILETAKHFTINYLENYMRSCKDEYEASIIRHALELPIHWRL 240

Query: 241 SRLDTRWFIDIYERKVDMNPILLEIAKLDFNQVQFIHQEDVKYASRWWRSTGFSEKFSFA 300
           SRLDTRWFIDIYERK DMNPILLE+AKLDFN+VQ IHQED+KYAS WWRSTGF +K SFA
Sbjct: 241 SRLDTRWFIDIYERKEDMNPILLELAKLDFNRVQSIHQEDLKYASSWWRSTGFGKKLSFA 300

Query: 301 RDRLMENFFWTVGVGFEPELSYFRRMATKVNALITTIDDIYDVYGTLDELELFTDAVERW 360
           RDRLMENFFWTVGVGFEPELSYFRRM  K+NAL T+IDD+YD+YGTLDELELFTDAVERW
Sbjct: 301 RDRLMENFFWTVGVGFEPELSYFRRMGVKINALTTSIDDVYDIYGTLDELELFTDAVERW 360

Query: 361 DVGTMDQLPDYMKICFFSLHNSINEMAFEALRDKGINVLKYLKKAWADLCQAYMLEAKWY 420
           DVG +DQLPDYMKICFF LHNSINEMAF+ALRDKG NV++YLKK W DLC+AYMLEA+WY
Sbjct: 361 DVGAVDQLPDYMKICFFVLHNSINEMAFDALRDKGTNVIQYLKKLWVDLCKAYMLEARWY 420

Query: 421 HTSYKPTLEEYLDNAWISISGPVILVHAYAFVTSPTLQDMESLVEYADIIRWSSTILRLA 480
           H+ YKPTLEEYLDNAWISISGPVIL+HAY FVTSPTLQDMESLVEYADIIRWSSTI+RLA
Sbjct: 421 HSRYKPTLEEYLDNAWISISGPVILIHAYVFVTSPTLQDMESLVEYADIIRWSSTIVRLA 480

Query: 481 NDLGTSSDELERGDILKSIQCYMNDTGASESKAREHVKRLIDETWKKLNKAEVENSIFSQ 540
           +DLGTSSDE++RGDI KS+QCYMNDTGASESKAREHV+ LIDETWKKLNK +VENS+F  
Sbjct: 481 DDLGTSSDEIKRGDIPKSVQCYMNDTGASESKAREHVRHLIDETWKKLNKIDVENSVFPP 540

Query: 541 VFIERAKNLARMAQCVYQYGDGHGMGYQEINDRVMSLLIRPISTHSYN 588
           VFI+ AKNLA+MAQC+YQ+GDGHG+G+QE N+ + SLLIRPIS  SY+
Sbjct: 541 VFIKIAKNLAKMAQCIYQHGDGHGLGHQETNEDIASLLIRPISIRSYS 588

BLAST of CmaCh20G010840 vs. TAIR 10
Match: AT3G25820.1 (terpene synthase-like sequence-1,8-cineole )

HSP 1 Score: 537.3 bits (1383), Expect = 1.5e-152
Identity = 273/549 (49.73%), Postives = 385/549 (70.13%), Query Frame = 0

Query: 46  RRSGNYKPPIWKLEFIESLKSEFKGEMYVEHLNELKEEIDIIMNQIIDDPLKQLELIDTL 105
           RRSGNY+P  W   ++ S+++++  E  V   + LK+++  +++      L+QLELID L
Sbjct: 47  RRSGNYQPSPWNHCYLLSIENKYASETEVITRDVLKKKVKSMLDDEKKSRLEQLELIDDL 106

Query: 106 QRLGISYHFENEIKDMLKRTYEK----SYESDDWKKNNLHATSLEFRLLRQHGFNVSQEV 165
           Q+LG+SYHFE EI D L   + K     ++ D  K+ +LHATSLEFRLLRQHGF+VS+ +
Sbjct: 107 QKLGVSYHFEIEINDTLTDLHLKMGRNCWKCD--KEEDLHATSLEFRLLRQHGFDVSENI 166

Query: 166 FNNFKDEMKNFSTRLYKDLNGMLSLYEASFLCIEGENIL-EAAKHFTINHLEKYM--RSC 225
           F+   D++++ +T    ++NG++SLYEAS+L  + +  L +  + F    + K++     
Sbjct: 167 FDVIIDQIES-NTFKTNNINGIISLYEASYLSTKSDTKLHKVIRPFATEQIRKFVDDEDT 226

Query: 226 KDEHEASIIRHALEFPLYWQISRLDTRWFIDIYERKVDMNPILLEIAKLDFNQVQFIHQE 285
           K+        HALE P +W++ RLDTRW+ID YE+K DMN +L+E AK+DFN VQ  HQE
Sbjct: 227 KNIEVREKAYHALEMPYHWRMRRLDTRWYIDAYEKKHDMNLVLIEFAKIDFNIVQAAHQE 286

Query: 286 DVKYASRWWRSTGFSEKFSFARDRLMENFFWTVGVGFEPELSYFRRMATKVNALITTIDD 345
           D+KY SRWW+ T  + +  F RDR++EN+FWTVG+ +EP+  Y RR+ T VNAL+TTIDD
Sbjct: 287 DLKYVSRWWKDTCLTNQLPFVRDRIVENYFWTVGLIYEPQFGYIRRIMTIVNALVTTIDD 346

Query: 346 IYDVYGTLDELELFTDAVERWDVGTMDQLPDYMKICFFSLHNSINEMAFEALRDKGINVL 405
           IYD+YGTL+ELELFT  VE WDV  + +LP+YM++CF  L+N IN +  + L+ K I+V+
Sbjct: 347 IYDIYGTLEELELFTSMVENWDVNRLGELPEYMRLCFLILYNEINGIGCDILKYKKIDVI 406

Query: 406 KYLKKAWADLCQAYMLEAKWYHTSYKPTLEEYLDNAWISISGPVILVHAY-AFVTSPTLQ 465
            YLKK+WADLC+ Y++EAKWY   YKP+LEEY+ NAWISIS P IL+H Y  F    ++Q
Sbjct: 407 PYLKKSWADLCRTYLVEAKWYKRGYKPSLEEYMQNAWISISAPTILIHFYCVFSDQISVQ 466

Query: 466 DMESLVEYAD-IIRWSSTILRLANDLGTSSDELERGDILKSIQCYMNDTGASESKAREHV 525
           ++E+L ++   I+R S+T+LRLANDLGTS  EL RGD+LKS+QCYM++TGASE +AR+HV
Sbjct: 467 NLETLSQHRQHIVRCSATVLRLANDLGTSPTELARGDVLKSVQCYMHETGASEERARDHV 526

Query: 526 KRLIDETWKKLN---KAEVENSIFSQVFIERAKNLARMAQCVYQYGDGHGMGYQ-EINDR 582
            ++I + W  +N   K    +S  S+ F E A NLARM+QC+YQYGDGHG   + +  DR
Sbjct: 527 HQMISDMWDDMNSETKTACNSSSRSRGFKEAAMNLARMSQCMYQYGDGHGCPEKAKTIDR 586

BLAST of CmaCh20G010840 vs. TAIR 10
Match: AT3G25830.1 (terpene synthase-like sequence-1,8-cineole )

HSP 1 Score: 537.3 bits (1383), Expect = 1.5e-152
Identity = 273/549 (49.73%), Postives = 385/549 (70.13%), Query Frame = 0

Query: 46  RRSGNYKPPIWKLEFIESLKSEFKGEMYVEHLNELKEEIDIIMNQIIDDPLKQLELIDTL 105
           RRSGNY+P  W   ++ S+++++  E  V   + LK+++  +++      L+QLELID L
Sbjct: 47  RRSGNYQPSPWNHCYLLSIENKYASETEVITRDVLKKKVKSMLDDEKKSRLEQLELIDDL 106

Query: 106 QRLGISYHFENEIKDMLKRTYEK----SYESDDWKKNNLHATSLEFRLLRQHGFNVSQEV 165
           Q+LG+SYHFE EI D L   + K     ++ D  K+ +LHATSLEFRLLRQHGF+VS+ +
Sbjct: 107 QKLGVSYHFEIEINDTLTDLHLKMGRNCWKCD--KEEDLHATSLEFRLLRQHGFDVSENI 166

Query: 166 FNNFKDEMKNFSTRLYKDLNGMLSLYEASFLCIEGENIL-EAAKHFTINHLEKYM--RSC 225
           F+   D++++ +T    ++NG++SLYEAS+L  + +  L +  + F    + K++     
Sbjct: 167 FDVIIDQIES-NTFKTNNINGIISLYEASYLSTKSDTKLHKVIRPFATEQIRKFVDDEDT 226

Query: 226 KDEHEASIIRHALEFPLYWQISRLDTRWFIDIYERKVDMNPILLEIAKLDFNQVQFIHQE 285
           K+        HALE P +W++ RLDTRW+ID YE+K DMN +L+E AK+DFN VQ  HQE
Sbjct: 227 KNIEVREKAYHALEMPYHWRMRRLDTRWYIDAYEKKHDMNLVLIEFAKIDFNIVQAAHQE 286

Query: 286 DVKYASRWWRSTGFSEKFSFARDRLMENFFWTVGVGFEPELSYFRRMATKVNALITTIDD 345
           D+KY SRWW+ T  + +  F RDR++EN+FWTVG+ +EP+  Y RR+ T VNAL+TTIDD
Sbjct: 287 DLKYVSRWWKDTCLTNQLPFVRDRIVENYFWTVGLIYEPQFGYIRRIMTIVNALVTTIDD 346

Query: 346 IYDVYGTLDELELFTDAVERWDVGTMDQLPDYMKICFFSLHNSINEMAFEALRDKGINVL 405
           IYD+YGTL+ELELFT  VE WDV  + +LP+YM++CF  L+N IN +  + L+ K I+V+
Sbjct: 347 IYDIYGTLEELELFTSMVENWDVNRLGELPEYMRLCFLILYNEINGIGCDILKYKKIDVI 406

Query: 406 KYLKKAWADLCQAYMLEAKWYHTSYKPTLEEYLDNAWISISGPVILVHAY-AFVTSPTLQ 465
            YLKK+WADLC+ Y++EAKWY   YKP+LEEY+ NAWISIS P IL+H Y  F    ++Q
Sbjct: 407 PYLKKSWADLCRTYLVEAKWYKRGYKPSLEEYMQNAWISISAPTILIHFYCVFSDQISVQ 466

Query: 466 DMESLVEYAD-IIRWSSTILRLANDLGTSSDELERGDILKSIQCYMNDTGASESKAREHV 525
           ++E+L ++   I+R S+T+LRLANDLGTS  EL RGD+LKS+QCYM++TGASE +AR+HV
Sbjct: 467 NLETLSQHRQHIVRCSATVLRLANDLGTSPTELARGDVLKSVQCYMHETGASEERARDHV 526

Query: 526 KRLIDETWKKLN---KAEVENSIFSQVFIERAKNLARMAQCVYQYGDGHGMGYQ-EINDR 582
            ++I + W  +N   K    +S  S+ F E A NLARM+QC+YQYGDGHG   + +  DR
Sbjct: 527 HQMISDMWDDMNSETKTACNSSSRSRGFKEAAMNLARMSQCMYQYGDGHGCPEKAKTIDR 586

BLAST of CmaCh20G010840 vs. TAIR 10
Match: AT3G25810.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 522.7 bits (1345), Expect = 3.8e-148
Identity = 266/554 (48.01%), Postives = 381/554 (68.77%), Query Frame = 0

Query: 46  RRSGNYKPPIWKLEFIESLKSEFKGEMYVEHLNELKEEIDIIMNQI-IDDPLKQLELIDT 105
           RRSGNY+P  W   ++ S+++++  E  V   + LK+++  ++ ++     L++LELID 
Sbjct: 47  RRSGNYQPSSWDHSYLLSIENKYVNEKEVITRHVLKKKVKKMLEEVETKSRLEKLELIDD 106

Query: 106 LQRLGISYHFENEIKDMLKRTYEKSYESDDW---KKNNLHATSLEFRLLRQHGFNVSQEV 165
           LQ+LG+SYHFE EI ++L   +      + W   K+ +LHAT+LEFRLLRQHGF VS+++
Sbjct: 107 LQKLGVSYHFEQEINNIL-TNFHLENGKNIWKCDKEEDLHATALEFRLLRQHGFGVSEDI 166

Query: 166 FNNFKDEMKNFSTRLYKDLNGMLSLYEASFLCIEGENIL-EAAKHFTINHLEKYMRSCKD 225
           F+   D++++ +T    ++  +++LYEAS+L  + +  L +  + F    +  ++    D
Sbjct: 167 FDVIIDKIES-NTFKSDNITSIITLYEASYLSTKSDTKLHKVIRPFATEQIRNFV---DD 226

Query: 226 EHEASIIR------HALEFPLYWQISRLDTRWFIDIYERKVDMNPILLEIAKLDFNQVQF 285
           E E   I       HALE P +W++ RL+TRW+ID YE+K DMN  L E AK+DFN VQ 
Sbjct: 227 ESETYNIMLREMAIHALEIPYHWRMRRLETRWYIDAYEKKHDMNLFLAEFAKIDFNIVQT 286

Query: 286 IHQEDVKYASRWWRSTGFSEKFSFARDRLMENFFWTVGVGFEPELSYFRRMATKVNALIT 345
            HQEDVKY S WW+ TG   +  F RDR++EN+FWTVG+ +EP+  Y RR+   V ALIT
Sbjct: 287 AHQEDVKYVSCWWKETGLGSQLHFVRDRIVENYFWTVGMIYEPQFGYIRRIVAIVAALIT 346

Query: 346 TIDDIYDVYGTLDELELFTDAVERWDVGTMDQLPDYMKICFFSLHNSINEMAFEALRDKG 405
            IDDIYD+YGT +ELELFT  V+ WD+  +D+LP+YMK+CF +L N IN M  + L+ K 
Sbjct: 347 VIDDIYDIYGTPEELELFTAMVQNWDINRLDELPEYMKLCFLTLFNEINAMGCDVLKCKN 406

Query: 406 INVLKYLKKAWADLCQAYMLEAKWYHTSYKPTLEEYLDNAWISISGPVILVHAY-AFVTS 465
           I+V+ Y KK+WADLC+AY++EAKWY   YKP++EEY+ NAWISIS P +L+H Y AF   
Sbjct: 407 IDVIPYFKKSWADLCKAYLVEAKWYKGGYKPSVEEYMQNAWISISAPTMLIHFYCAFSGQ 466

Query: 466 PTLQDMESLV-EYADIIRWSSTILRLANDLGTSSDELERGDILKSIQCYMNDTGASESKA 525
            ++Q +ESLV +  D++R S+T+LRLANDL TS DEL RGD+LKS+QCYM++TG SE +A
Sbjct: 467 ISVQILESLVQQQQDVVRCSATVLRLANDLATSPDELARGDVLKSVQCYMHETGVSEEEA 526

Query: 526 REHVKRLIDETWKKLN----KAEVENSIFSQVFIERAKNLARMAQCVYQYGDGHGM-GYQ 582
           R HV+++I  TW ++N     A   +S+ S+ F+E A NLARM+QC+YQ+GDGHG     
Sbjct: 527 RTHVQQMISHTWDEMNYEARTAARSSSLLSRRFVETAMNLARMSQCMYQHGDGHGCPDKA 586

BLAST of CmaCh20G010840 vs. TAIR 10
Match: AT4G16740.1 (terpene synthase 03 )

HSP 1 Score: 515.4 bits (1326), Expect = 6.1e-146
Identity = 264/553 (47.74%), Postives = 376/553 (67.99%), Query Frame = 0

Query: 40  SSQTIVRRSGNYKPPIWKLEFIESLKSEFKGEMYVEHLNELKEEIDIIMNQIIDDPLKQL 99
           S   + RRS NY+P +W+ E++ SL + +  E  VE +  LK+E+  ++N+  +  L+QL
Sbjct: 20  SQPLVSRRSANYQPSLWQHEYLLSLGNTYVKEDNVERVTLLKQEVSKMLNE-TEGLLEQL 79

Query: 100 ELIDTLQRLGISYHFENEIKDMLKRTYEKSYES-----DDWKKNNLHATSLEFRLLRQHG 159
           ELIDTLQRLG+SYHFE EIK  L   + K+  +     D  +  +L+AT+LEFRLLRQHG
Sbjct: 80  ELIDTLQRLGVSYHFEQEIKKTLTNVHVKNVRAHKNRIDRNRWGDLYATALEFRLLRQHG 139

Query: 160 FNVSQEVFNNFKDEMKNFSTRL-YKDLNGMLSLYEASFLCIEGENILEAAKHFTINHLEK 219
           F+++Q+VF+       N    L  KD+ G+LSLYEAS+L    +  L+ + ++T   L K
Sbjct: 140 FSIAQDVFDG------NIGVDLDDKDIKGILSLYEASYLSTRIDTKLKESIYYTTKRLRK 199

Query: 220 YMRSCKDEHEASIIR----HALEFPLYWQISRLDTRWFIDIYERKVDMNPILLEIAKLDF 279
           ++   K+E ++  +R    HALE P + ++ RL+ RW+I++Y  + DMNPILLE+AKLDF
Sbjct: 200 FVEVNKNETKSYTLRRMVIHALEMPYHRRVGRLEARWYIEVYGERHDMNPILLELAKLDF 259

Query: 280 NQVQFIHQEDVKYASRWWRSTGFSEKFSFARDRLMENFFWTVGVGFEPELSYFRRMATKV 339
           N VQ IHQ+++K  S WW  TG ++   F RDR+ E +F +VGV +EPE +Y R+M TKV
Sbjct: 260 NFVQAIHQDELKSLSSWWSKTGLTKHLDFVRDRITEGYFSSVGVMYEPEFAYHRQMLTKV 319

Query: 340 NALITTIDDIYDVYGTLDELELFTDAVERWDVGTMDQLPDYMKICFFSLHNSINEMAFEA 399
             LITTIDDIYD+YGTL+EL+LFT  VE+WDV  +++LP+YMK+CF  L N IN++ +  
Sbjct: 320 FMLITTIDDIYDIYGTLEELQLFTTIVEKWDVNRLEELPNYMKLCFLCLVNEINQIGYFV 379

Query: 400 LRDKGINVLKYLKKAWADLCQAYMLEAKWYHTSYKPTLEEYLDNAWISISGPVILVHAYA 459
           LRDKG NV+ YLK++WAD+C  ++ EAKWY + YKP  EEY+ N WIS S P IL+H + 
Sbjct: 380 LRDKGFNVIPYLKESWADMCTTFLKEAKWYKSGYKPNFEEYMQNGWISSSVPTILLHLFC 439

Query: 460 FVTSPTLQDMESLVEYADIIRWSSTILRLANDLGTSSDELERGDILKSIQCYMNDTGASE 519
            ++  TL  + S      ++R S+TILRLANDL TSS+EL RGD +KS+QC+M++TGASE
Sbjct: 440 LLSDQTLDILGSYNH--SVVRSSATILRLANDLATSSEELARGDTMKSVQCHMHETGASE 499

Query: 520 SKAREHVKRLIDETWKKLNKAEVENSIFSQVFIERAKNLARMAQCVYQYGDGHGM-GYQE 579
           +++R +++ +I   W  LN  E ++    Q F+E A NL R+AQCVYQYGDGHG     +
Sbjct: 500 AESRAYIQGIIGVAWDDLN-MEKKSCRLHQGFLEAAANLGRVAQCVYQYGDGHGCPDKAK 559

Query: 580 INDRVMSLLIRPI 582
             + V SLL+ P+
Sbjct: 560 TVNHVRSLLVHPL 562

BLAST of CmaCh20G010840 vs. TAIR 10
Match: AT2G24210.1 (terpene synthase 10 )

HSP 1 Score: 513.8 bits (1322), Expect = 1.8e-145
Identity = 264/553 (47.74%), Postives = 379/553 (68.54%), Query Frame = 0

Query: 41  SQTIVRRSGNYKPPIWKLEFIESLKSEFKGEMYVEHLNELKEEIDIIMNQIIDDPLKQLE 100
           S T+ RRS NY+P  W    + S++++F  +  V   + LKE++  ++N      L QLE
Sbjct: 41  SPTVQRRSANYQPSRWDHHHLLSVENKFAKDKRVRERDLLKEKVRKMLNDEQKTYLDQLE 100

Query: 101 LIDTLQRLGISYHFENEIKDMLKRTYEKSYESDDWKKNNLHATSLEFRLLRQHGFNVSQE 160
            ID LQ+LG+SYHFE EI ++L  +Y+K  +  + ++++LHAT+LEFRL RQHGFNVS++
Sbjct: 101 FIDDLQKLGVSYHFEAEIDNILTSSYKK--DRTNIQESDLHATALEFRLFRQHGFNVSED 160

Query: 161 VFNNFKDEMKNFSTRLYKDLNGMLSLYEASFLCIE-GENILEAAKHFTINHLEKYMRSCK 220
           VF+ F +    F      D+ G++SLYEAS+L  +  +N+    + F    L  ++ +  
Sbjct: 161 VFDVFMENCGKFDR---DDIYGLISLYEASYLSTKLDKNLQIFIRPFATQQLRDFVDTHS 220

Query: 221 DEHEAS-----IIRHALEFPLYWQISRLDTRWFIDIY-ERKVDMNPILLEIAKLDFNQVQ 280
           +E   S     I+  AL+ P YWQ+ RL TRW+ID+Y +R+   N +++E AK+DFN VQ
Sbjct: 221 NEDFGSCDMVEIVVQALDMPYYWQMRRLSTRWYIDVYGKRQNYKNLVVVEFAKIDFNIVQ 280

Query: 281 FIHQEDVKYASRWWRSTGFSEKFSFARDRLMENFFWTVGVGFEPELSYFRRMATKVNALI 340
            IHQE++K  S WW  TG  ++  FARDR++EN+FWT+G   EP+  Y R+  TK+NAL+
Sbjct: 281 AIHQEELKNVSSWWMETGLGKQLYFARDRIVENYFWTIGQIQEPQYGYVRQTMTKINALL 340

Query: 341 TTIDDIYDVYGTLDELELFTDAVERWDVGTMDQLPDYMKICFFSLHNSINEMAFEALRDK 400
           TTIDDIYD+YGTL+EL+LFT A E WD+  +D+LP+YM++CF  ++N +N +A E LR K
Sbjct: 341 TTIDDIYDIYGTLEELQLFTVAFENWDINRLDELPEYMRLCFLVIYNEVNSIACEILRTK 400

Query: 401 GINVLKYLKKAWADLCQAYMLEAKWYHTSYKPTLEEYLDNAWISISGPVILVHAY-AFVT 460
            INV+ +LKK+W D+ +AY++EAKWY + +KP LEEY+ NA ISIS P I VH Y  F  
Sbjct: 401 NINVIPFLKKSWTDVSKAYLVEAKWYKSGHKPNLEEYMQNARISISSPTIFVHFYCVFSD 460

Query: 461 SPTLQDMESLVEY-ADIIRWSSTILRLANDLGTSSDELERGDILKSIQCYMNDTGASESK 520
             ++Q +E+L ++  +++R SS++ RLANDL TS DEL RGD+ KSIQCYM++TGASE K
Sbjct: 461 QLSIQVLETLSQHQQNVVRCSSSVFRLANDLVTSPDELARGDVCKSIQCYMSETGASEDK 520

Query: 521 AREHVKRLIDETWKKLN--KAEVENSIFSQVFIERAKNLARMAQCVYQYGDGHGMGYQ-E 580
           AR HV+++I++ W ++N  K    +SI    F+E   NLARM+QC+YQYGDGHG   + +
Sbjct: 521 ARSHVRQMINDLWDEMNYEKMAHSSSILHHDFMETVINLARMSQCMYQYGDGHGSPEKAK 580

Query: 581 INDRVMSLLIRPI 582
           I DRVMSLL  PI
Sbjct: 581 IVDRVMSLLFNPI 588

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
B9T5361.6e-20461.83Terpene synthase 10 OS=Ricinus communis OX=3988 GN=TPS10 PE=2 SV=1[more]
Q93X231.1e-20060.59Myrcene synthase, chloroplastic OS=Quercus ilex OX=58334 PE=1 SV=1[more]
Q6PWU22.2e-19356.56(-)-alpha-terpineol synthase OS=Vitis vinifera OX=29760 PE=1 SV=1[more]
B6SCF44.4e-17352.38Myrcene synthase, chloroplastic OS=Humulus lupulus OX=3486 PE=1 SV=1[more]
Q8L5K44.8e-17252.77Gamma-terpinene synthase, chloroplastic OS=Citrus limon OX=2708 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1I2T80.0e+00100.00terpene synthase 10-like OS=Cucurbita maxima OX=3661 GN=LOC111470415 PE=4 SV=1[more]
A0A6J1EMY10.0e+0092.02terpene synthase 10-like OS=Cucurbita moschata OX=3662 GN=LOC111434992 PE=4 SV=1[more]
A0A6J1EJW92.7e-29081.29terpene synthase 10-like OS=Cucurbita moschata OX=3662 GN=LOC111434993 PE=4 SV=1[more]
A0A6J1EJ261.6e-27177.25terpene synthase 10-like OS=Cucurbita moschata OX=3662 GN=LOC111434910 PE=4 SV=1[more]
A0A6J1I4361.6e-26678.22terpene synthase 10-like OS=Cucurbita maxima OX=3661 GN=LOC111470418 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022971747.10.0e+00100.00terpene synthase 10-like [Cucurbita maxima][more]
XP_023512342.10.0e+0092.36terpene synthase 10-like [Cucurbita pepo subsp. pepo][more]
XP_022928103.10.0e+0092.02terpene synthase 10-like [Cucurbita moschata][more]
XP_022928104.15.5e-29081.29terpene synthase 10-like [Cucurbita moschata][more]
XP_023513213.16.3e-28680.44terpene synthase 10-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G25820.11.5e-15249.73terpene synthase-like sequence-1,8-cineole [more]
AT3G25830.11.5e-15249.73terpene synthase-like sequence-1,8-cineole [more]
AT3G25810.13.8e-14848.01Terpenoid cyclases/Protein prenyltransferases superfamily protein [more]
AT4G16740.16.1e-14647.74terpene synthase 03 [more]
AT2G24210.11.8e-14547.74terpene synthase 10 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005630Terpene synthase, metal-binding domainPFAMPF03936Terpene_synth_Ccoord: 262..526
e-value: 4.2E-99
score: 331.2
IPR008949Isoprenoid synthase domain superfamilyGENE3D1.10.600.10Farnesyl Diphosphate Synthasecoord: 53..571
e-value: 4.7E-213
score: 710.2
IPR008949Isoprenoid synthase domain superfamilySUPERFAMILY48576Terpenoid synthasescoord: 257..581
IPR036965Terpene synthase, N-terminal domain superfamilyGENE3D1.50.10.130coord: 93..257
e-value: 4.7E-213
score: 710.2
IPR001906Terpene synthase, N-terminal domainPFAMPF01397Terpene_synthcoord: 56..231
e-value: 1.4E-51
score: 174.9
NoneNo IPR availableSFLDSFLDS00005Isoprenoid_Synthase_Type_Icoord: 257..582
e-value: 0.0
score: 395.2
NoneNo IPR availablePANTHERPTHR31225OS04G0344100 PROTEIN-RELATEDcoord: 25..583
NoneNo IPR availablePANTHERPTHR31225:SF114TERPENE SYNTHASE 10-LIKEcoord: 25..583
IPR034741Terpene cyclase-like 1, C-terminal domainSFLDSFLDG01019Terpene_Cyclase_Like_1_C_Termcoord: 257..582
e-value: 0.0
score: 395.2
IPR044814Terpene cyclases, class 1, plantCDDcd00684Terpene_cyclase_plant_C1coord: 46..580
e-value: 0.0
score: 671.983
IPR008930Terpenoid cyclases/protein prenyltransferase alpha-alpha toroidSUPERFAMILY48239Terpenoid cyclases/Protein prenyltransferasescoord: 53..254

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh20G010840.1CmaCh20G010840.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016102 diterpenoid biosynthetic process
biological_process GO:0032784 regulation of DNA-templated transcription, elongation
biological_process GO:0006357 regulation of transcription by RNA polymerase II
biological_process GO:0006368 transcription elongation from RNA polymerase II promoter
cellular_component GO:0032044 DSIF complex
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0003729 mRNA binding
molecular_function GO:0010333 terpene synthase activity
molecular_function GO:0016829 lyase activity