CmaCh20G007450 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh20G007450
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Phytocyanin domain-containing protein
Location	Cma_Chr20: 3496253 .. 3498326 (+)
RNA-Seq Expression	CmaCh20G007450
Synteny	CmaCh20G007450

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTTGCTTAAAGATTAAACCTACTTATATCTTCTTCTCTCTCTGTGTGTGTCTGACTTAGAGAAAGGGGTTTTGGAGTGAGAAACAATGATGATGATGATGGTGAAGTTGTTGATGTTTCTTAGCTTTGGTGGACTGTCAATGTGTGAGGTGTTTCTTGTTGGAGACGAAGAAGGCTGGAACTCTGGCATCAATTTTGCTACCTGGTCTCAAAACCACAATTTCACCAAAGGAGATTTTCTAGGTATTCATCAACTTCTCTCTCTTTCTTCCATCTTCAAACCGAGTAGCTTTTAGGTCGTGCTAGAGTATTTGAACTTCTAACCTCTTTGTCGATGGATTAGTTCGTTTGTTGATTGAAACTGTTGAGTACTAGTGATTGTAAGATTCTATGTCGGTTGGAGAGGGCAACGAAACATTTCTTATAAGTGTGTGGAAACCTCTCCCTAACCAATGTGTTTTAAAAACAGTGAGACTTATAGCGATATGTAACGAGTCAAAGCAGACAATATTTGCTAGCGGTTGGCTTGGGTTGTTACAAATGGTATTAGAGCCAGAATTCGGGTGGTGTGCCAACGAAGACGTTGGGTCCCCAAAGGAAGTGTATTCTGAGATTTCACATCAGTTGGAGAAGGGAACGAAACATTCTTTATAAGGGTGCGGAAACCTCTCCCCAGCATACATGTTTTAAAAATTGTAAGGCTGACGACGATTTGTAACTGGCCAAAGCGAATAATATCTACTATTGGTGAGCTTGGGCTGTTACAAATGGTGTCAAAGCTAGACACGGAACGGTGTGCCAGCGAGGACATTGGGCCAACAAGAGGGGTGAATTGTGAGATCTCACATCGGTTGGAGAATGGAACGAAGCATTCCTTATAAGGGTGCGGAAACCTCTCCATAGCATACGCGTTTTAAAAACTGTGAGATTGATTACAATATGTAACTGGCCAAAGCAGATAATGTCTACTAGTGGTGGGCTTGAGCTGTTACAAATGGTGTCAAAGTCAGACACTGAACGGTGTGCCAGCGAGGACGTTGGGCCACTAAGAGGGGTGGATTGTGAGAACCCACATCACTGGAAGAAGGGAACGAAACATTCCTTATAAAAGTGTAGAAATTTCTCTCTAGTAAACGCATTTTAAAATCATGAAGCTGACGGCGATACATAACATGTCAAAGTAGACAATATTTTCCAGCAGTGAGTTTGGGCTGTTACAAATGGTATCAGAGCTAGACACTGAGTTGTATGCTAGCGAGGACGTTAGGCCCCTAAGGAGAGTGGATTGTGAGATCCCACATCAATGAAGAAGATAACGAAATATTTTTTATAAAGGTGTGAAAACTTATTCCTAGCAGACACGTTTTAAAACCGTGAGGCTAACGACGATACGTAACGGGTCAAAGTAGACAATATCTACTAGCGATAAGGTTGAGCTGTTATAGTGATTTTAACGTGACCTGATCTCTCGGGATTGAATATGTAGTTGTTCGATTAATGTTTTAGTTTTAATTTTTAACGATCGTGTTATAGTTTTTGATTTCGAGTTTACGAAAAATTCATGTAGTTTTCAATTATGCAAAGAGTGTACACAATGTATATGAAGTTAGTGAGGAAACATTCAGATCATGCGAGACAAGTAATGGAGTATTGGGTGAATATGAGAGTGGAAATGATCGAATTGAGCTGAAAGAAGCAAGGAAGTATTGGTTCATATGTAATGTAGCTGGTCATTGCTTGGGCGGAATGAGGTTTGGAATTGATGTCAATGAAGCCAATCCTAGGAGCCATCTCCCACTTAGCCCTGTTCAATCTCCTCCCCCACCCACCAACCATGCCTCTCACCATGGAAGCTATGCACTATGGAGCTCTTTTATGGGTATGGTAGCATTTCAAACTCTGTTCGTCGAACAGTATCGGTAGTGCAGTTTTGAGATTTGAGTTCTTACCTTGCAACAGAAGAATGTTACTGATGTCTTAACTCTTTCGAGGGTTGTTCAAATTTGTTACTTTTGACCGTCGTTGAATGAATACGAGATTTGAGTTCTTGACTTGCAACAGAAGAATGTTACTG

mRNA sequence

GTTTGCTTAAAGATTAAACCTACTTATATCTTCTTCTCTCTCTGTGTGTGTCTGACTTAGAGAAAGGGGTTTTGGAGTGAGAAACAATGATGATGATGATGGTGAAGTTGTTGATGTTTCTTAGCTTTGGTGGACTGTCAATGTGTGAGGTGTTTCTTGTTGGAGACGAAGAAGGCTGGAACTCTGGCATCAATTTTGCTACCTGGTCTCAAAACCACAATTTCACCAAAGGAGATTTTCTAGTTTTCAATTATGCAAAGAGTGTACACAATGTATATGAAGTTAGTGAGGAAACATTCAGATCATGCGAGACAAGTAATGGAGTATTGGGTGAATATGAGAGTGGAAATGATCGAATTGAGCTGAAAGAAGCAAGGAAGTATTGGTTCATATGTAATGTAGCTGGTCATTGCTTGGGCGGAATGAGGTTTGGAATTGATGTCAATGAAGCCAATCCTAGGAGCCATCTCCCACTTAGCCCTGTTCAATCTCCTCCCCCACCCACCAACCATGCCTCTCACCATGGAAGCTATGCACTATGGAGCTCTTTTATGGGTATGGTAGCATTTCAAACTCTGTTCGTCGAACAGTATCGGTAGTGCAGTTTTGAGATTTGAGTTCTTACCTTGCAACAGAAGAATGTTACTGATGTCTTAACTCTTTCGAGGGTTGTTCAAATTTGTTACTTTTGACCGTCGTTGAATGAATACGAGATTTGAGTTCTTGACTTGCAACAGAAGAATGTTACTG

Coding sequence (CDS)

ATGATGATGATGATGGTGAAGTTGTTGATGTTTCTTAGCTTTGGTGGACTGTCAATGTGTGAGGTGTTTCTTGTTGGAGACGAAGAAGGCTGGAACTCTGGCATCAATTTTGCTACCTGGTCTCAAAACCACAATTTCACCAAAGGAGATTTTCTAGTTTTCAATTATGCAAAGAGTGTACACAATGTATATGAAGTTAGTGAGGAAACATTCAGATCATGCGAGACAAGTAATGGAGTATTGGGTGAATATGAGAGTGGAAATGATCGAATTGAGCTGAAAGAAGCAAGGAAGTATTGGTTCATATGTAATGTAGCTGGTCATTGCTTGGGCGGAATGAGGTTTGGAATTGATGTCAATGAAGCCAATCCTAGGAGCCATCTCCCACTTAGCCCTGTTCAATCTCCTCCCCCACCCACCAACCATGCCTCTCACCATGGAAGCTATGCACTATGGAGCTCTTTTATGGGTATGGTAGCATTTCAAACTCTGTTCGTCGAACAGTATCGGTAG

Protein sequence

MMMMMVKLLMFLSFGGLSMCEVFLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSVHNVYEVSEETFRSCETSNGVLGEYESGNDRIELKEARKYWFICNVAGHCLGGMRFGIDVNEANPRSHLPLSPVQSPPPPTNHASHHGSYALWSSFMGMVAFQTLFVEQYR

Homology

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BLAST of CmaCh20G007450 vs. ExPASy Swiss-Prot
Match: Q41001 (Blue copper protein OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 2.0e-17
Identity = 47/127 (37.01%), Postives = 71/127 (55.91%), Query Frame = 0


Query: 3   MMMVKLLMFLSFGGLSMCEVFLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSVHN 62
           +++  LL  ++    S+  V+ VGD  GW  G +++TW+ +  F  GD LVFNY    H 
Sbjct: 7   LVLCFLLAIINMALPSLATVYTVGDTSGWVIGGDYSTWASDKTFAVGDSLVFNYGAGAHT 66

Query: 63  VYEVSEETFRSCETSNGVLGEYESGNDRIELKEARKYWFICNVAGHCLGGMRFGIDVNEA 122
           V EV E  ++SC + N +  +  +G   I LK+A K++FIC V GH  GGM+  I V  +
Sbjct: 67  VDEVKESDYKSCTSGNSISTD-STGATTIPLKKAGKHYFICGVPGHSTGGMKLSIKVKAS 126

Query: 123 NPRSHLP 130
           +  S  P
Sbjct: 127 SGSSAAP 132

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BLAST of CmaCh20G007450 vs. ExPASy Swiss-Prot
Match: P80728 (Mavicyanin OS=Cucurbita pepo OX=3663 PE=1 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 2.0e-14
Identity = 39/101 (38.61%), Postives = 58/101 (57.43%), Query Frame = 0


Query: 22  VFLVGDEEGWNSGI--NFATWSQNHNFTKGDFLVFNYAKSVHNVYEVSEETFRSCETSNG 81
           V  VGD  GW + +  ++A W+ ++ F  GD L+FNY    HNV +V +E F+SC +S+ 
Sbjct: 3   VHKVGDSTGWTTLVPYDYAKWASSNKFHVGDSLLFNYNNKFHNVLQVDQEQFKSCNSSSP 62

Query: 82  VLGEYESGNDRIELKEARKYWFICNVAGHCLGGMRFGIDVN 121
               Y SG D I LK    ++F+C + GHC  G +  I V+
Sbjct: 63  A-ASYTSGADSIPLKRPGTFYFLCGIPGHCQLGQKVEIKVD 102

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BLAST of CmaCh20G007450 vs. ExPASy Swiss-Prot
Match: P00303 (Basic blue protein OS=Cucumis sativus OX=3659 PE=1 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 6.6e-13
Identity = 38/97 (39.18%), Postives = 54/97 (55.67%), Query Frame = 0


Query: 22  VFLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSVHNVYEVSEETFRSCETSNGVL 81
           V++VG   GW    N  +W +   F  GD L+FNY   +HNV  V++  F +C T  G  
Sbjct: 2   VYVVGGSGGWT--FNTESWPKGKRFRAGDILLFNYNPXMHNVVVVNQGGFSTCNTPAGA- 61

Query: 82  GEYESGNDRIELKEARKYWFICNVAGHCLGGMRFGID 119
             Y SG D+I+L + + Y FICN  GHC  GM+  ++
Sbjct: 62  KVYTSGRDQIKLPKGQSY-FICNFPGHCQSGMKIAVN 94

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BLAST of CmaCh20G007450 vs. ExPASy Swiss-Prot
Match: Q96316 (Uclacyanin-3 OS=Arabidopsis thaliana OX=3702 GN=UCC3 PE=2 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 8.6e-13
Identity = 44/137 (32.12%), Postives = 65/137 (47.45%), Query Frame = 0


Query: 8   LLMFLSFGGLSMCEVFLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSVHNVYEVS 67
           LL+FL+         F VGD  GW S +++  W     F  GD L F Y  S H+V  V 
Sbjct: 9   LLLFLAAVPAVFAATFKVGDISGWTSNLDYTVWLTGKTFRVGDTLEFVYGLS-HSVSVVD 68

Query: 68  EETFRSCETSNGVLGEYESGNDRIELKEARKYWFICNVAGHCLGGMRFGIDVNEANPRSH 127
           +  + +C+ S+G    +  G+ +I+L       F+C   GHC  GM+  + V  A P   
Sbjct: 69  KAGYDNCD-SSGATQNFADGDTKIDLTTVGTMHFLCPTFGHCKNGMKLAVPVLAAAPSPS 128

Query: 128 LPLSPVQSPPPPTNHAS 145
            P SP  +P  P++  S
Sbjct: 129 TPSSPPSTPSTPSSPPS 143

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BLAST of CmaCh20G007450 vs. ExPASy Swiss-Prot
Match: A0A072U307 (Blue copper protein 1b OS=Medicago truncatula OX=3880 GN=BCP1B PE=2 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 3.3e-12
Identity = 54/166 (32.53%), Postives = 84/166 (50.60%), Query Frame = 0


Query: 1   MMMMMVKLLMFLSFGGLSMCEV----FLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNY 60
           M    V L++ +S   LS   +    ++VGD++GW    ++  W+Q+  F  GD LVFNY
Sbjct: 1   MASSRVVLILSISMVLLSSVAIAATDYIVGDDKGWTVDFDYTQWAQDKVFRVGDNLVFNY 60

Query: 61  AKSVHNVYEVSEETFRSC--ETSNGVLGEYESGNDRIELKEARKYWFICNVAGHCLG-GM 120
             S HNV++V+   F+SC     N  L    +G D I+LK   + W++C VA HC    M
Sbjct: 61  DPSRHNVFKVNGTLFQSCTFPPKNEAL---STGKDIIQLKTEGRKWYVCGVADHCSARQM 120

Query: 121 RFGIDVNEANPRSHLPLSPVQSPPPPTNHASHHGSYALWSSFMGMV 160
           +  I V           +P  SPPP ++      ++++ SS  G+V
Sbjct: 121 KLVITVLAEG-------APAPSPPPSSD------AHSVVSSLFGVV 150

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BLAST of CmaCh20G007450 vs. ExPASy TrEMBL
Match: A0A6J1JF60 (basic blue protein-like OS=Cucurbita maxima OX=3661 GN=LOC111483949 PE=4 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 2.7e-94
Identity = 170/170 (100.00%), Postives = 170/170 (100.00%), Query Frame = 0


Query: 1   MMMMMVKLLMFLSFGGLSMCEVFLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSV 60
           MMMMMVKLLMFLSFGGLSMCEVFLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSV
Sbjct: 1   MMMMMVKLLMFLSFGGLSMCEVFLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSV 60

Query: 61  HNVYEVSEETFRSCETSNGVLGEYESGNDRIELKEARKYWFICNVAGHCLGGMRFGIDVN 120
           HNVYEVSEETFRSCETSNGVLGEYESGNDRIELKEARKYWFICNVAGHCLGGMRFGIDVN
Sbjct: 61  HNVYEVSEETFRSCETSNGVLGEYESGNDRIELKEARKYWFICNVAGHCLGGMRFGIDVN 120

Query: 121 EANPRSHLPLSPVQSPPPPTNHASHHGSYALWSSFMGMVAFQTLFVEQYR 171
           EANPRSHLPLSPVQSPPPPTNHASHHGSYALWSSFMGMVAFQTLFVEQYR
Sbjct: 121 EANPRSHLPLSPVQSPPPPTNHASHHGSYALWSSFMGMVAFQTLFVEQYR 170

[Close]

BLAST of CmaCh20G007450 vs. ExPASy TrEMBL
Match: A0A6J1FST3 (basic blue protein-like OS=Cucurbita moschata OX=3662 GN=LOC111448452 PE=4 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 6.0e-86
Identity = 155/164 (94.51%), Postives = 161/164 (98.17%), Query Frame = 0


Query: 3   MMMVKLLMFLSFGGLSMCEVFLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSVHN 62
           MMMVKL MFLSFGGLSMCEVFLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSVHN
Sbjct: 1   MMMVKLFMFLSFGGLSMCEVFLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSVHN 60

Query: 63  VYEVSEETFRSCETSNGVLGEYESGNDRIELKEARKYWFICNVAGHCLGGMRFGIDVNEA 122
           VYEV+EETFRSCETSNGVLGEYESGND+IELKEARKYWFICNVAGHCLGGMRFGIDVNEA
Sbjct: 61  VYEVTEETFRSCETSNGVLGEYESGNDQIELKEARKYWFICNVAGHCLGGMRFGIDVNEA 120

Query: 123 NPRSHLPLSPVQSPPPPTNHASHHGSYALWSSFMGMVAFQTLFV 167
           N RSHLPLSPVQSPPPPTNHAS+HGSYALWS+FM ++AFQTLFV
Sbjct: 121 NSRSHLPLSPVQSPPPPTNHASNHGSYALWSTFMCIIAFQTLFV 164

[Close]

BLAST of CmaCh20G007450 vs. ExPASy TrEMBL
Match: A0A1S3C699 (mavicyanin-like OS=Cucumis melo OX=3656 GN=LOC103497341 PE=4 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 6.3e-59
Identity = 118/165 (71.52%), Postives = 134/165 (81.21%), Query Frame = 0


Query: 4   MMVKL----------LMFLSFGGLSMCEVFLVGDEEGWNSGINFATWSQNHNFTKGDFLV 63
           MMVKL           +FLSF G+SMCEVF+VGDE+GWNSGINFATWSQ+HNFTKGDFLV
Sbjct: 1   MMVKLYHNCRLIYYFFLFLSFFGVSMCEVFVVGDEDGWNSGINFATWSQSHNFTKGDFLV 60

Query: 64  FNYAKSVHNVYEVSEETFRSCETSNGVLGEYESGNDRIELKEARKYWFICNVAGHCLGGM 123
           FNYAK+VHNVYEV EET+RSCE  NGVLGEY+SGND+IELKE R YWFICNV GHCLGGM
Sbjct: 61  FNYAKNVHNVYEVIEETYRSCEAKNGVLGEYDSGNDKIELKEGRNYWFICNVVGHCLGGM 120

Query: 124 RFGIDV-NEANPRSHLPLSPV-QSPPPPTNHAS-HHGSYALWSSF 156
           RFGI+V  + N  +HLPL+P+ QSPPP TNHAS   G Y +W +F
Sbjct: 121 RFGIEVKQQPNSSTHLPLNPIDQSPPPNTNHASICCGRYPMWWTF 165

[Close]

BLAST of CmaCh20G007450 vs. ExPASy TrEMBL
Match: A0A0A0LME3 (Phytocyanin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G270850 PE=4 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 2.4e-58
Identity = 111/150 (74.00%), Postives = 129/150 (86.00%), Query Frame = 0


Query: 19  MCEVFLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSVHNVYEVSEETFRSCETSN 78
           MCEVF+VGDE+GWNSG NFATWSQ+HNFTKGDFLVFNYAK+VHNVYEV EET+RSCE  N
Sbjct: 1   MCEVFVVGDEDGWNSGTNFATWSQSHNFTKGDFLVFNYAKNVHNVYEVIEETYRSCEAKN 60

Query: 79  GVLGEYESGNDRIELKEARKYWFICNVAGHCLGGMRFGIDVNEANPRSHLPLSPV-QSPP 138
           GVLGEY+SGND+IELKEAR YWFICNVAGHCLGGMRFGI V E+N  +HLPL+P+ QSPP
Sbjct: 61  GVLGEYDSGNDKIELKEARNYWFICNVAGHCLGGMRFGIVVKESNSSTHLPLNPIDQSPP 120

Query: 139 PPTNHAS-HHGSYAL-WSSFMGMVAFQTLF 166
           P TNHAS   G + + W+ F+ +++F  LF
Sbjct: 121 PNTNHASICCGRFPMWWTFFICILSFNVLF 150

[Close]

BLAST of CmaCh20G007450 vs. ExPASy TrEMBL
Match: A0A6J1CYL0 (basic blue protein-like OS=Momordica charantia OX=3673 GN=LOC111015378 PE=4 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 5.3e-58
Identity = 113/161 (70.19%), Postives = 130/161 (80.75%), Query Frame = 0


Query: 8   LLMFLSFGG--LSMCEVFLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSVHNVYE 67
           L +FLSFGG  LS CEVFLVG E+GWNSGINF +WSQ HNFTKGD LVFNY+K+ HNVYE
Sbjct: 14  LFLFLSFGGILLSKCEVFLVGGEDGWNSGINFVSWSQTHNFTKGDVLVFNYSKNEHNVYE 73

Query: 68  VSEETFRSCETSNGVLGEYESGNDRIELKEARKYWFICNVAGHCLGGMRFGIDVNEANPR 127
           VSEET+RSC  S GV+GEY+SGND IELKEARKYWFICNVAGHCLGGMRFGI+VN+ N  
Sbjct: 74  VSEETYRSCNASKGVVGEYDSGNDEIELKEARKYWFICNVAGHCLGGMRFGIEVNQPNST 133

Query: 128 SHLPLSPVQSPPPPTNHASHHGSYALWSS-FMGMVAFQTLF 166
            H+P S  Q PPP TNH S+  SY +WSS  + ++AF  +F
Sbjct: 134 IHVPNSDFQPPPPTTNHGSYE-SYQIWSSTLIFIIAFGLVF 173

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BLAST of CmaCh20G007450 vs. NCBI nr
Match: XP_022986108.1 (basic blue protein-like [Cucurbita maxima] >XP_022986109.1 basic blue protein-like [Cucurbita maxima])

HSP 1 Score: 354.4 bits (908), Expect = 5.6e-94
Identity = 170/170 (100.00%), Postives = 170/170 (100.00%), Query Frame = 0


Query: 1   MMMMMVKLLMFLSFGGLSMCEVFLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSV 60
           MMMMMVKLLMFLSFGGLSMCEVFLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSV
Sbjct: 1   MMMMMVKLLMFLSFGGLSMCEVFLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSV 60

Query: 61  HNVYEVSEETFRSCETSNGVLGEYESGNDRIELKEARKYWFICNVAGHCLGGMRFGIDVN 120
           HNVYEVSEETFRSCETSNGVLGEYESGNDRIELKEARKYWFICNVAGHCLGGMRFGIDVN
Sbjct: 61  HNVYEVSEETFRSCETSNGVLGEYESGNDRIELKEARKYWFICNVAGHCLGGMRFGIDVN 120

Query: 121 EANPRSHLPLSPVQSPPPPTNHASHHGSYALWSSFMGMVAFQTLFVEQYR 171
           EANPRSHLPLSPVQSPPPPTNHASHHGSYALWSSFMGMVAFQTLFVEQYR
Sbjct: 121 EANPRSHLPLSPVQSPPPPTNHASHHGSYALWSSFMGMVAFQTLFVEQYR 170

[Close]

BLAST of CmaCh20G007450 vs. NCBI nr
Match: XP_022943841.1 (basic blue protein-like [Cucurbita moschata])

HSP 1 Score: 326.6 bits (836), Expect = 1.2e-85
Identity = 155/164 (94.51%), Postives = 161/164 (98.17%), Query Frame = 0


Query: 3   MMMVKLLMFLSFGGLSMCEVFLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSVHN 62
           MMMVKL MFLSFGGLSMCEVFLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSVHN
Sbjct: 1   MMMVKLFMFLSFGGLSMCEVFLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSVHN 60

Query: 63  VYEVSEETFRSCETSNGVLGEYESGNDRIELKEARKYWFICNVAGHCLGGMRFGIDVNEA 122
           VYEV+EETFRSCETSNGVLGEYESGND+IELKEARKYWFICNVAGHCLGGMRFGIDVNEA
Sbjct: 61  VYEVTEETFRSCETSNGVLGEYESGNDQIELKEARKYWFICNVAGHCLGGMRFGIDVNEA 120

Query: 123 NPRSHLPLSPVQSPPPPTNHASHHGSYALWSSFMGMVAFQTLFV 167
           N RSHLPLSPVQSPPPPTNHAS+HGSYALWS+FM ++AFQTLFV
Sbjct: 121 NSRSHLPLSPVQSPPPPTNHASNHGSYALWSTFMCIIAFQTLFV 164

[Close]

BLAST of CmaCh20G007450 vs. NCBI nr
Match: KAG6571015.1 (hypothetical protein SDJN03_29930, partial [Cucurbita argyrosperma subsp. sororia] >KAG7010846.1 hypothetical protein SDJN02_27642, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 318.9 bits (816), Expect = 2.6e-83
Identity = 151/164 (92.07%), Postives = 158/164 (96.34%), Query Frame = 0


Query: 3   MMMVKLLMFLSFGGLSMCEVFLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSVHN 62
           MMMVKL MFLSFGGLSMCEVFLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSVHN
Sbjct: 1   MMMVKLFMFLSFGGLSMCEVFLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSVHN 60

Query: 63  VYEVSEETFRSCETSNGVLGEYESGNDRIELKEARKYWFICNVAGHCLGGMRFGIDVNEA 122
           VYEV+EETFRSCETSNGVLGEYESGND+IELKEARKYWFICNVAGHCLGGMRFGIDV + 
Sbjct: 61  VYEVTEETFRSCETSNGVLGEYESGNDQIELKEARKYWFICNVAGHCLGGMRFGIDVKQP 120

Query: 123 NPRSHLPLSPVQSPPPPTNHASHHGSYALWSSFMGMVAFQTLFV 167
           N  SHLPLSPVQSPPPPTNHAS+HGSYALWS+FM ++AFQTLFV
Sbjct: 121 NSSSHLPLSPVQSPPPPTNHASNHGSYALWSTFMCIIAFQTLFV 164

[Close]

BLAST of CmaCh20G007450 vs. NCBI nr
Match: XP_023513085.1 (basic blue protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 317.8 bits (813), Expect = 5.8e-83
Identity = 151/166 (90.96%), Postives = 159/166 (95.78%), Query Frame = 0


Query: 1   MMMMMVKLLMFLSFGGLSMCEVFLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSV 60
           MMMMMVKLLMFLSFGGLSMCEVFLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSV
Sbjct: 1   MMMMMVKLLMFLSFGGLSMCEVFLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSV 60

Query: 61  HNVYEVSEETFRSCETSNGVLGEYESGNDRIELKEARKYWFICNVAGHCLGGMRFGIDVN 120
           HNVYEV+EETFRSCETSNGVLGEYESGND+IELKEARKYWFICNVAGHCLGGMRFGIDV 
Sbjct: 61  HNVYEVTEETFRSCETSNGVLGEYESGNDQIELKEARKYWFICNVAGHCLGGMRFGIDVK 120

Query: 121 EANPRSHLPLSPVQSPPPPTNHASHHGSYALWSSFMGMVAFQTLFV 167
           + N  SHLP SPVQSPPPPTNH S+HGSYA+WS+FM ++AFQTLFV
Sbjct: 121 QPNSTSHLPPSPVQSPPPPTNHGSNHGSYAIWSTFMCIIAFQTLFV 166

[Close]

BLAST of CmaCh20G007450 vs. NCBI nr
Match: XP_038902627.1 (mavicyanin-like [Benincasa hispida])

HSP 1 Score: 263.8 bits (673), Expect = 9.9e-67
Identity = 121/163 (74.23%), Postives = 146/163 (89.57%), Query Frame = 0


Query: 4   MMVKLLMFLSFGGLSMCEVFLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSVHNV 63
           +++   +FLSF G+S+CEVFLVGDE+GWNSGINFATWSQNH FTKGDFLVFNYAK+VHNV
Sbjct: 11  LIIHFFLFLSFCGISICEVFLVGDEDGWNSGINFATWSQNHTFTKGDFLVFNYAKNVHNV 70

Query: 64  YEVSEETFRSCETSNGVLGEYESGNDRIELKEARKYWFICNVAGHCLGGMRFGIDVNEAN 123
           YEV+EET+RSCETSNGVLG+Y+SGND+IELKEAR YWFICNVAGHCLGGMRFGI+V EAN
Sbjct: 71  YEVTEETYRSCETSNGVLGKYDSGNDKIELKEARNYWFICNVAGHCLGGMRFGIEVKEAN 130

Query: 124 PRSHLPLSPVQSPPPPTNHAS-HHGSYALWSSFMGMVAFQTLF 166
              HLPL+P+QSPPP TN+AS + G Y++W+ F+ ++AF+ LF
Sbjct: 131 SSIHLPLNPIQSPPPNTNYASINCGRYSMWTPFICILAFKVLF 173

[Close]

BLAST of CmaCh20G007450 vs. TAIR 10
Match: AT3G17675.1 (Cupredoxin superfamily protein )

HSP 1 Score: 84.3 bits (207), Expect = 1.0e-16
Identity = 39/96 (40.62%), Postives = 49/96 (51.04%), Query Frame = 0


Query: 24  LVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSVHNVYEVSEETFRSCETSNGVLGE 83
           +VGD  GW    N+  W+Q   F  GD LVFNY    HNV +V+   +  C   N     
Sbjct: 10  IVGDSNGWELFTNYTNWTQGREFHVGDVLVFNYKSDQHNVMQVNSTAYTDCGLDN-YTTL 69

Query: 84  YESGNDRIELKEARKYWFICNVAGHCLGGMRFGIDV 120
           +  GND I L E  K WFIC V  HC+ G +  I+V
Sbjct: 70  FTKGNDSIILSEVGKLWFICGVDDHCVNGQKLSINV 104

[Close]

BLAST of CmaCh20G007450 vs. TAIR 10
Match: AT5G26330.1 (Cupredoxin superfamily protein )

HSP 1 Score: 82.0 bits (201), Expect = 5.0e-16
Identity = 53/157 (33.76%), Postives = 75/157 (47.77%), Query Frame = 0


Query: 17  LSMCEVFLVGDEEGWN--SGINFATWSQNHNFTKGDFLVFNYAKSVHNVYEVSEETFRSC 76
           LS   V+ VGD  GW   + +++  W+    F  GD ++F Y    HNV  V+   +RSC
Sbjct: 18  LSEAAVYKVGDSAGWTTIANVDYKLWASTKTFHIGDTVLFEYNPQFHNVMRVTHPMYRSC 77

Query: 77  ETSNGVLGEYESGNDRIELKEARKYWFICNVAGHCLGGMRFGIDV---NEANPRSHLPLS 136
            TS  +   + +GND I L     ++F C V GHCL G +  + V     + P S  P S
Sbjct: 78  NTSKPI-STFTTGNDSITLTNHGHHFFFCGVPGHCLAGQKLDLHVLLPASSTPLSDPPTS 137

Query: 137 PVQSPPPPTNHAS--HHGSYALWSSFMGMVAFQTLFV 167
              SPP  T  A+     S +L +S   MV  Q + V
Sbjct: 138 SSSSPPSTTIPAAGVPGPSPSLAASLPSMVTAQIVAV 173

[Close]

BLAST of CmaCh20G007450 vs. TAIR 10
Match: AT2G27035.1 (early nodulin-like protein 20 )

HSP 1 Score: 75.5 bits (184), Expect = 4.7e-14
Identity = 39/119 (32.77%), Postives = 61/119 (51.26%), Query Frame = 0


Query: 26  GDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSVHNVYEVSEETFRSCETSNGVLGEYE 85
           G    WNS +NF+ W+ +  F  GD+L F + ++ HN+ +V++ ++  C  ++ +     
Sbjct: 32  GGRYTWNSDVNFSDWANHQRFYSGDWLYFGFNRTRHNILQVNKSSYEQCVDNDYIFNITR 91

Query: 86  SGNDRIELKEARKYWFICNVAGHCLGGMRFGIDVNEANPRSHLPLSPVQSPPPPTNHAS 145
            G D  +L E + Y+FIC   G+CL GM+  I V        LP  P   P  PTN  S
Sbjct: 92  GGRDVFQLLEPKPYYFICG-RGYCLKGMKLAITV--------LPQPP---PSAPTNFTS 138

[Close]

BLAST of CmaCh20G007450 vs. TAIR 10
Match: AT3G60280.1 (uclacyanin 3 )

HSP 1 Score: 75.1 bits (183), Expect = 6.1e-14
Identity = 44/137 (32.12%), Postives = 65/137 (47.45%), Query Frame = 0


Query: 8   LLMFLSFGGLSMCEVFLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYAKSVHNVYEVS 67
           LL+FL+         F VGD  GW S +++  W     F  GD L F Y  S H+V  V 
Sbjct: 9   LLLFLAAVPAVFAATFKVGDISGWTSNLDYTVWLTGKTFRVGDTLEFVYGLS-HSVSVVD 68

Query: 68  EETFRSCETSNGVLGEYESGNDRIELKEARKYWFICNVAGHCLGGMRFGIDVNEANPRSH 127
           +  + +C+ S+G    +  G+ +I+L       F+C   GHC  GM+  + V  A P   
Sbjct: 69  KAGYDNCD-SSGATQNFADGDTKIDLTTVGTMHFLCPTFGHCKNGMKLAVPVLAAAPSPS 128

Query: 128 LPLSPVQSPPPPTNHAS 145
            P SP  +P  P++  S
Sbjct: 129 TPSSPPSTPSTPSSPPS 143

[Close]

BLAST of CmaCh20G007450 vs. TAIR 10
Match: AT3G27200.1 (Cupredoxin superfamily protein )

HSP 1 Score: 74.7 bits (182), Expect = 7.9e-14
Identity = 50/164 (30.49%), Postives = 84/164 (51.22%), Query Frame = 0


Query: 3   MMMVKLLMFLSFGGL-----SMCEVFLVGDEEGWNSGINFATWSQNHNFTKGDFLVFNYA 62
           M M  +L+ L F GL     ++    ++G  +GW   ++F +WS + +F  GD +VF Y+
Sbjct: 1   MKMQAVLVILVFSGLLSVKTALAARHVIGGSQGWEQSVDFDSWSSDQSFKVGDQIVFKYS 60

Query: 63  KSVHNVYEVSEET-FRSCETSNGVLGEYESGNDRIELKEARKYWFICNVAGHCLGGMRFG 122
           + +H+V E+  ET ++SC+    V     SGND ++L +    +F C   GHC  GM+  
Sbjct: 61  E-LHSVVELGSETAYKSCDLGTSV-NSLSSGNDVVKLSKTGTRYFACGTVGHCEQGMKIK 120

Query: 123 IDVNEANPRSHLPLSPVQSPPPPTNHASHHGSYALWSSFMGMVA 161
           ++V  ++ +S        S P  +   S  GS +  SS  G+ A
Sbjct: 121 VNVVSSDSKS-------ASSPSGSGSGSDSGSGSGSSSGHGLRA 155

The following BLAST results are available for this feature:

BLAST of CmaCh20G007450 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q41001	2.0e-17	37.01	Blue copper protein OS=Pisum sativum OX=3888 PE=2 SV=1	[more]
P80728	2.0e-14	38.61	Mavicyanin OS=Cucurbita pepo OX=3663 PE=1 SV=1	[more]
P00303	6.6e-13	39.18	Basic blue protein OS=Cucumis sativus OX=3659 PE=1 SV=1	[more]
Q96316	8.6e-13	32.12	Uclacyanin-3 OS=Arabidopsis thaliana OX=3702 GN=UCC3 PE=2 SV=1	[more]
A0A072U307	3.3e-12	32.53	Blue copper protein 1b OS=Medicago truncatula OX=3880 GN=BCP1B PE=2 SV=1	[more]

BLAST of CmaCh20G007450 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JF60	2.7e-94	100.00	basic blue protein-like OS=Cucurbita maxima OX=3661 GN=LOC111483949 PE=4 SV=1	[more]
A0A6J1FST3	6.0e-86	94.51	basic blue protein-like OS=Cucurbita moschata OX=3662 GN=LOC111448452 PE=4 SV=1	[more]
A0A1S3C699	6.3e-59	71.52	mavicyanin-like OS=Cucumis melo OX=3656 GN=LOC103497341 PE=4 SV=1	[more]
A0A0A0LME3	2.4e-58	74.00	Phytocyanin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G270850...	[more]
A0A6J1CYL0	5.3e-58	70.19	basic blue protein-like OS=Momordica charantia OX=3673 GN=LOC111015378 PE=4 SV=1	[more]

BLAST of CmaCh20G007450 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022986108.1	5.6e-94	100.00	basic blue protein-like [Cucurbita maxima] >XP_022986109.1 basic blue protein-li...	[more]
XP_022943841.1	1.2e-85	94.51	basic blue protein-like [Cucurbita moschata]	[more]
KAG6571015.1	2.6e-83	92.07	hypothetical protein SDJN03_29930, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023513085.1	5.8e-83	90.96	basic blue protein-like [Cucurbita pepo subsp. pepo]	[more]
XP_038902627.1	9.9e-67	74.23	mavicyanin-like [Benincasa hispida]	[more]

BLAST of CmaCh20G007450 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G17675.1	1.0e-16	40.63	Cupredoxin superfamily protein	[more]
AT5G26330.1	5.0e-16	33.76	Cupredoxin superfamily protein	[more]
AT2G27035.1	4.7e-14	32.77	early nodulin-like protein 20	[more]
AT3G60280.1	6.1e-14	32.12	uclacyanin 3	[more]
AT3G27200.1	7.9e-14	30.49	Cupredoxin superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 19..124 e-value: 5.5E-32 score: 112.1
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 20..119
IPR003245	Phytocyanin domain	PFAM	PF02298	Cu_bind_like	coord: 31..113 e-value: 2.9E-22 score: 78.5
IPR003245	Phytocyanin domain	PROSITE	PS51485	PHYTOCYANIN	coord: 21..121 score: 35.208031
IPR039391	Phytocyanin	PANTHER	PTHR33021	BLUE COPPER PROTEIN	coord: 3..132
None	No IPR available	PANTHER	PTHR33021:SF179	BLUE COPPER BINDING PROTEIN-LIKE	coord: 3..132
None	No IPR available	CDD	cd04216	Phytocyanin	coord: 22..119 e-value: 1.49453E-42 score: 135.466

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh20G007450.1	CmaCh20G007450.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0022900	electron transport chain
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0009055	electron transfer activity