CmaCh20G005110 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh20G005110
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionheavy metal-associated isoprenylated plant protein 3
LocationCma_Chr20: 2488187 .. 2489325 (-)
RNA-Seq ExpressionCmaCh20G005110
SyntenyCmaCh20G005110
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCAAGGTCAGTGGTCACTCCATTCTTCTTCCTCTGCTCTAATCCATTTCCCTTACTTTCTCTCTGTTTACAGAAGAAGAACGGCAGCCATGGAAGCGACAAAGAAGAAGACAGTACCACCGCCGTGTTCAACATCGATATGCACTGCGAGGGCTGCGCCAACAAACTCAGAAGGTGTGTCCGACAAATCCAAGGTATCATCAATTCCCGATTACCCTTTTTTCTTCTCAATATTATAAAGCAGATTCCAAACCCAATCCTATTTTTCCATTTACTGAAGGCGTTGAGAGAGTGAGAGCCGATTGGGAAGCCAACAAGCTAACAGTGATCGGAAAATTCGACGCGTCGTCGCTCCGAGAGAAGCTCGCCGATAAAACTAAGAAGAAAATCGACATTGTCTCGTTGGAGGGGAAGAAAGAAAAGGGGTCAAAGAAACCAGAGGACGAGAAGCCCAAAGACGAAGAGGTGAGTGAATACCTCTCATGGTTAGAGTAAATGAGGTGAGTCAACTCGGGATTTTTAAATTTATTTGTTACTTTTTAAATTATTCCGACCAGGCTCCGGCGACGACAGCGACGTTGAAGGTGGAACTGCACTGCCAGGGTTGCATCGAGAGGATTTACAAAGTCGTATCCAGGACCAAGGGTGAGATTTAATTATTGCTCATCAAATTAATTTTGGAGTTGAAGAGAATAATTAATGGGTTTTTTTTTTTTTTTTTTGCAGGAGTGGAGGATATGGCGATGGAGACACAAAAGGATTTGGTTACGGTGAAAGGGAAAATGGACGTCAAGGCTCTGATTCACAATTTGGAAGAGAAACTGAAGCGGAAAGTTGCAGTGGTGGTACCCAAAAAGGACAAAGACGACGGAGATGGCGGCGACAAGAACAAAAGCGGCGGAGAGGTCGCTCAACAGGATGGCGGCGGAGAAACGGACGGCGACAGACTTGATTACATGGCAGTTCCGGCTCCGGGTTATGAGTACGGGTTCGGGTACGGGTACGTGTACGGTCATGGTGGATTCGTTGGAGAACATTTGCCTTCGTCTCAAATGTTTAGCGATGAGAATCCGAATGCTTGCACAGTTATGTGATAAATATAGTTTTTTTTTTCTTTTAAATAAATGTAATTCGGC

mRNA sequence

ATGGGCAAGAAGAAGAACGGCAGCCATGGAAGCGACAAAGAAGAAGACAGTACCACCGCCGTGTTCAACATCGATATGCACTGCGAGGGCTGCGCCAACAAACTCAGAAGGTGTGTCCGACAAATCCAAGGCGTTGAGAGAGTGAGAGCCGATTGGGAAGCCAACAAGCTAACAGTGATCGGAAAATTCGACGCGTCGTCGCTCCGAGAGAAGCTCGCCGATAAAACTAAGAAGAAAATCGACATTGTCTCGTTGGAGGGGAAGAAAGAAAAGGGGTCAAAGAAACCAGAGGACGAGAAGCCCAAAGACGAAGAGGCTCCGGCGACGACAGCGACGTTGAAGGTGGAACTGCACTGCCAGGGTTGCATCGAGAGGATTTACAAAGTCGTATCCAGGACCAAGGGAGTGGAGGATATGGCGATGGAGACACAAAAGGATTTGGTTACGGTGAAAGGGAAAATGGACGTCAAGGCTCTGATTCACAATTTGGAAGAGAAACTGAAGCGGAAAGTTGCAGTGGTGGTACCCAAAAAGGACAAAGACGACGGAGATGGCGGCGACAAGAACAAAAGCGGCGGAGAGGTCGCTCAACAGGATGGCGGCGGAGAAACGGACGGCGACAGACTTGATTACATGGCAGTTCCGGCTCCGGGTTATGAGTACGGGTTCGGGTACGGGTACGTGTACGGTCATGGTGGATTCGTTGGAGAACATTTGCCTTCGTCTCAAATGTTTAGCGATGAGAATCCGAATGCTTGCACAGTTATGTGATAAATATAGTTTTTTTTTTCTTTTAAATAAATGTAATTCGGC

Coding sequence (CDS)

ATGGGCAAGAAGAAGAACGGCAGCCATGGAAGCGACAAAGAAGAAGACAGTACCACCGCCGTGTTCAACATCGATATGCACTGCGAGGGCTGCGCCAACAAACTCAGAAGGTGTGTCCGACAAATCCAAGGCGTTGAGAGAGTGAGAGCCGATTGGGAAGCCAACAAGCTAACAGTGATCGGAAAATTCGACGCGTCGTCGCTCCGAGAGAAGCTCGCCGATAAAACTAAGAAGAAAATCGACATTGTCTCGTTGGAGGGGAAGAAAGAAAAGGGGTCAAAGAAACCAGAGGACGAGAAGCCCAAAGACGAAGAGGCTCCGGCGACGACAGCGACGTTGAAGGTGGAACTGCACTGCCAGGGTTGCATCGAGAGGATTTACAAAGTCGTATCCAGGACCAAGGGAGTGGAGGATATGGCGATGGAGACACAAAAGGATTTGGTTACGGTGAAAGGGAAAATGGACGTCAAGGCTCTGATTCACAATTTGGAAGAGAAACTGAAGCGGAAAGTTGCAGTGGTGGTACCCAAAAAGGACAAAGACGACGGAGATGGCGGCGACAAGAACAAAAGCGGCGGAGAGGTCGCTCAACAGGATGGCGGCGGAGAAACGGACGGCGACAGACTTGATTACATGGCAGTTCCGGCTCCGGGTTATGAGTACGGGTTCGGGTACGGGTACGTGTACGGTCATGGTGGATTCGTTGGAGAACATTTGCCTTCGTCTCAAATGTTTAGCGATGAGAATCCGAATGCTTGCACAGTTATGTGA

Protein sequence

MGKKKNGSHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKFDASSLREKLADKTKKKIDIVSLEGKKEKGSKKPEDEKPKDEEAPATTATLKVELHCQGCIERIYKVVSRTKGVEDMAMETQKDLVTVKGKMDVKALIHNLEEKLKRKVAVVVPKKDKDDGDGGDKNKSGGEVAQQDGGGETDGDRLDYMAVPAPGYEYGFGYGYVYGHGGFVGEHLPSSQMFSDENPNACTVM
Homology
BLAST of CmaCh20G005110 vs. ExPASy Swiss-Prot
Match: Q9FJH5 (Heavy metal-associated isoprenylated plant protein 3 OS=Arabidopsis thaliana OX=3702 GN=HIPP03 PE=1 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 4.4e-53
Identity = 131/279 (46.95%), Postives = 168/279 (60.22%), Query Frame = 0

Query: 3   KKKNGSHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGK 62
           KKK G +    E  S T V  +DMHCEGCA+++ +CVR  QGVE V+++    KLTV G 
Sbjct: 11  KKKGGDNKKKNETPSITVVLKVDMHCEGCASRIVKCVRSFQGVETVKSESATGKLTVTGA 70

Query: 63  FDASSLREKLADKTKKKIDIVSLEGKKEK----------------GSKKPEDEKPKDEEA 122
            D   LREKL +KTKKK+D+VS + KKEK                  KKP++   K +E 
Sbjct: 71  LDPVKLREKLEEKTKKKVDLVSPQPKKEKEKENKNKNDEDKKKSEEKKKPDNNDKKPKET 130

Query: 123 PATTATLKVELHCQGCIERIYKVVSRTKGVEDMAMETQKDLVTVKGKMDVKALIHNLEEK 182
           P TTA LK+  HCQGCI +I K V++TKGV  + M+ +K+L+TVKG MDVK L+  L EK
Sbjct: 131 PVTTAVLKLNFHCQGCIGKIQKTVTKTKGVNGLTMDKEKNLLTVKGTMDVKKLVEILSEK 190

Query: 183 LKRKVAVVVPKKDKDDGDG---GDKNKSGG------EVAQQDGGGETDGDRLDYMAVPAP 242
           LKR V +V PKK+KD  +G   G+K K GG      E     GGGE   + ++YMA   P
Sbjct: 191 LKRAVEIVPPKKEKDKENGNENGEKKKGGGGDGGGKEKTGNKGGGE-GVNMMEYMAA-QP 250

Query: 243 GYEYGFGYGYVYGHGGFVGEHLPSSQMFSDENPNACTVM 257
            Y YG+  G  YG+   +  H P  Q+FSDENPNAC VM
Sbjct: 251 AYGYGYYPGGPYGYP--IQAHAP--QIFSDENPNACVVM 283

BLAST of CmaCh20G005110 vs. ExPASy Swiss-Prot
Match: Q9SJL2 (Heavy metal-associated isoprenylated plant protein 5 OS=Arabidopsis thaliana OX=3702 GN=HIPP05 PE=1 SV=2)

HSP 1 Score: 137.1 bits (344), Expect = 2.8e-31
Identity = 118/359 (32.87%), Postives = 161/359 (44.85%), Query Frame = 0

Query: 2   GKKKNGSHGSDKEEDSTTA------VFNIDMHCEGCANKLRRCVRQIQGVERVRADWEAN 61
           GK K+G   S        A      V+ +DMHCEGCA K++R V+   GV+ V AD   N
Sbjct: 28  GKPKSGGGDSAAAAAPPVAAVVSAFVYKVDMHCEGCAKKIKRMVKHFDGVKDVTADTGGN 87

Query: 62  KLTVIGKFDASSLREKLADKTKKKIDIVS----LEG---------KKEKGSKKPEDEKPK 121
           KL V+GK D   L+EKL +KTK+K+ + +    +EG         K + G K+     P 
Sbjct: 88  KLLVVGKIDPVKLQEKLEEKTKRKVVLANPPPKVEGPVAAAVGEKKADGGDKEAAPPAPA 147

Query: 122 DEEAPATTATLKVELHCQGCIERIYKVVSRTKGVEDMAMETQKDLVTVKGKMDVKALIHN 181
                 +   LK+ LHC+GCI++I K++ + KGVE +A++  KD+VTVKG +DVK L+  
Sbjct: 148 PAAPKESVVPLKIRLHCEGCIQKIKKIILKIKGVETVAIDGAKDVVTVKGTIDVKELVPL 207

Query: 182 LEEKLKRKVAVVVP----------------------------------------KKDKDD 241
           L +KLKR V  +VP                                        +K K+ 
Sbjct: 208 LTKKLKRTVEPLVPAKKDDGAAENKKTEAAAPDAKKEAPSAGVNEAKKEGSDGGEKKKEV 267

Query: 242 GDGGDKNKSGGEVAQ------------QDGGG-------ETDGDRLDYMAVP-AP----- 257
           GDGG+K K GG+  +            +DGGG           D   Y A P AP     
Sbjct: 268 GDGGEKKKEGGDGGEKKKEAGDGGEKKKDGGGVPAPVAMVNKMDYYGYSAYPTAPMHWQE 327

BLAST of CmaCh20G005110 vs. ExPASy Swiss-Prot
Match: Q9LZF1 (Heavy metal-associated isoprenylated plant protein 6 OS=Arabidopsis thaliana OX=3702 GN=HIPP06 PE=1 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 5.2e-30
Identity = 122/392 (31.12%), Postives = 169/392 (43.11%), Query Frame = 0

Query: 1   MGKKKNGS----HGSDKEEDS--TTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEA 60
           MG+KK  +     G  K  D   TT V  +DMHCEGC  K++R  +  +GVE V+ D+++
Sbjct: 1   MGEKKEETATKPQGEKKPTDGGITTVVMKLDMHCEGCGKKIKRIFKHFKGVEDVKIDYKS 60

Query: 61  NKLTVIGKFDASSLREKLADKTKKKIDIVSL----------------------------- 120
           NKLTVIG  D   +R+K+ADK K+ +++VS                              
Sbjct: 61  NKLTVIGNVDPVEVRDKVADKIKRPVELVSTVAPPKKETPPSSGGAEKKPSPAAEEKPAE 120

Query: 121 --------EGKKEKGSKKPEDEKPKDEEAP--ATTATLKVELHCQGCIERIYKVVSRTKG 180
                    G+K++  KK E EK      P   +T  LK +LHC+GC  +I ++V++ KG
Sbjct: 121 KKPAAVEKPGEKKEEKKKEEGEKKASPPPPPKESTVVLKTKLHCEGCEHKIKRIVNKIKG 180

Query: 181 VEDMAMETQKDLVTVKGKMDVKALIHNLEEKLKRKVAVVVPKKD---------------- 240
           V  +A+++ KDLV VKG +DVK L   L EKLKR V VV  KKD                
Sbjct: 181 VNSVAIDSAKDLVIVKGIIDVKQLTPYLNEKLKRTVEVVPAKKDDGAPVAAAAAAPAGGE 240

Query: 241 ---------KDDGDGGDKNKSGGEVAQQD---------GGGETDGDRLD--------YMA 257
                    K+  D G+K   GG   +++         GGG  DG  +D        Y  
Sbjct: 241 KKDKVAGEKKEIKDVGEKKVDGGGEKKKEVAVGGGGGGGGGGGDGGAMDVKKSEYNGYGY 300

BLAST of CmaCh20G005110 vs. ExPASy Swiss-Prot
Match: Q9ZUV1 (Heavy metal-associated isoprenylated plant protein 1 OS=Arabidopsis thaliana OX=3702 GN=HIPP01 PE=2 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 1.5e-29
Identity = 95/254 (37.40%), Postives = 141/254 (55.51%), Query Frame = 0

Query: 13  KEEDSTTA----VFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIG-KFDASS 72
           K++++TT+    V  ID HC+GC  ++ R  R+++GVE VRAD ++NKLT+IG   D   
Sbjct: 20  KKQNNTTSPVHVVLKIDFHCDGCIARIVRLSRRLEGVETVRADPDSNKLTLIGFIMDPVK 79

Query: 73  LREKLADKTKKKIDIVSLEGKKEKGSKKPEDEKPKDEE---APATTATLKVELHCQGCIE 132
           + EKL  K+KKK++++S + KK+  +K+  ++K  D+       TT  LKV   C GCI+
Sbjct: 80  IAEKLQKKSKKKVELISPKPKKD--TKENNEKKANDKTQTVVAVTTVVLKVNCSCDGCIK 139

Query: 133 RIYKVVSRTKGVEDMAMETQKDLVTVKGKMDVKALIHNLEEKLKRKVAVV-VPKKDKDDG 192
           RI K VS TKGV  + M+ +K+ VTV G MD+K++  NL+ KLK+ V VV   KK K D 
Sbjct: 140 RIQKAVSTTKGVYQVKMDKEKETVTVMGTMDIKSVTDNLKRKLKKTVQVVPEKKKKKKDK 199

Query: 193 DGGDKNKSGGEVAQQDGGGETDGDRLDYMAVPAPGYEYGFG-YGYVYGHGGFVGEHLPSS 252
           D  + N   G   Q                 P  G  +G G Y ++ G         P +
Sbjct: 200 DNAEVNTKVGSPCQ-----------------PGNGCTHGIGPYRFMEG---------PMT 245

Query: 253 QMFSDENPNACTVM 257
             FS+E+ + C+VM
Sbjct: 260 GFFSEEDQSYCSVM 245

BLAST of CmaCh20G005110 vs. ExPASy Swiss-Prot
Match: Q9FLU5 (Heavy metal-associated isoprenylated plant protein 9 OS=Arabidopsis thaliana OX=3702 GN=HIPP09 PE=2 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 3.2e-19
Identity = 76/263 (28.90%), Postives = 121/263 (46.01%), Query Frame = 0

Query: 21  VFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKFDASSLREKLADKTKKKI 80
           +  +D+HC GCA K+ R + +I+GVE V  D   N++T+ G  D  ++  K+  KTK+  
Sbjct: 59  ILYVDLHCVGCAKKIERSILKIRGVEEVVMDMNENQVTIKGVLDPQAVCNKIKKKTKRMA 118

Query: 81  DIVSLEGKKEKGSKKPEDEKPKDEEAPATTATLKVELHCQGCIERIYKVVSRTKGVEDMA 140
            ++S     E     P            TT  L V +HCQ C +++ K + + +GV+   
Sbjct: 119 KVLSPLPAAEGEPLPP--IITSQVSGGLTTVELSVNMHCQACADQLKKKILKMRGVQTTV 178

Query: 141 METQKDLVTVKGKMDVKALIHNLEEKLKRKVAVV-------------VPKKDKDDGDGGD 200
            E     V V G MD + L+  +  + K++  +V               +K ++ G+G +
Sbjct: 179 TEHTTGKVIVTGTMDAEKLVDYVYRRTKKQARIVPQPDPEPEAPAAAQEEKKEESGEGNE 238

Query: 201 K-----------NKSGGEVAQQDGGGE----TDGDRLDYMAVPAPGYEYGFGYGYVYGHG 256
           K            K  GE   ++GGGE    T+ +R D   + A   E G      Y   
Sbjct: 239 KPPETGEEKEEEKKKEGEENGEEGGGEEAAATEEERRD-NEMTAMAQEEGMKRMMYYYQP 298

BLAST of CmaCh20G005110 vs. ExPASy TrEMBL
Match: A0A6J1J8L7 (heavy metal-associated isoprenylated plant protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111483606 PE=4 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 4.1e-139
Identity = 256/256 (100.00%), Postives = 256/256 (100.00%), Query Frame = 0

Query: 1   MGKKKNGSHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVI 60
           MGKKKNGSHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVI
Sbjct: 1   MGKKKNGSHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVI 60

Query: 61  GKFDASSLREKLADKTKKKIDIVSLEGKKEKGSKKPEDEKPKDEEAPATTATLKVELHCQ 120
           GKFDASSLREKLADKTKKKIDIVSLEGKKEKGSKKPEDEKPKDEEAPATTATLKVELHCQ
Sbjct: 61  GKFDASSLREKLADKTKKKIDIVSLEGKKEKGSKKPEDEKPKDEEAPATTATLKVELHCQ 120

Query: 121 GCIERIYKVVSRTKGVEDMAMETQKDLVTVKGKMDVKALIHNLEEKLKRKVAVVVPKKDK 180
           GCIERIYKVVSRTKGVEDMAMETQKDLVTVKGKMDVKALIHNLEEKLKRKVAVVVPKKDK
Sbjct: 121 GCIERIYKVVSRTKGVEDMAMETQKDLVTVKGKMDVKALIHNLEEKLKRKVAVVVPKKDK 180

Query: 181 DDGDGGDKNKSGGEVAQQDGGGETDGDRLDYMAVPAPGYEYGFGYGYVYGHGGFVGEHLP 240
           DDGDGGDKNKSGGEVAQQDGGGETDGDRLDYMAVPAPGYEYGFGYGYVYGHGGFVGEHLP
Sbjct: 181 DDGDGGDKNKSGGEVAQQDGGGETDGDRLDYMAVPAPGYEYGFGYGYVYGHGGFVGEHLP 240

Query: 241 SSQMFSDENPNACTVM 257
           SSQMFSDENPNACTVM
Sbjct: 241 SSQMFSDENPNACTVM 256

BLAST of CmaCh20G005110 vs. ExPASy TrEMBL
Match: A0A6J1FUL5 (heavy metal-associated isoprenylated plant protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111448569 PE=4 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 7.3e-128
Identity = 240/262 (91.60%), Postives = 247/262 (94.27%), Query Frame = 0

Query: 1   MGKKKNGSHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVI 60
           MGKK+NGSHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVI
Sbjct: 1   MGKKQNGSHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVI 60

Query: 61  GKFDASSLREKLADKTKKKIDIVSLEGKKEKGSKKP----EDEKPKDEEAPATTATLKVE 120
           GKFDAS+LREKLADKT KKIDIVS EGKKEK SKKP    EDEKPKD+E P TTATLKVE
Sbjct: 61  GKFDASTLREKLADKTNKKIDIVSSEGKKEKKSKKPDEEAEDEKPKDKEIPVTTATLKVE 120

Query: 121 LHCQGCIERIYKVVSRTKGVEDMAMETQKDLVTVKGKMDVKALIHNLEEKLKRKVAVVVP 180
           LHCQGC+ERIYKVVSRTKGVEDMAME QKDLVTVKGKMDVKAL+HNLE+KLKRKVAVVVP
Sbjct: 121 LHCQGCVERIYKVVSRTKGVEDMAMERQKDLVTVKGKMDVKALVHNLEDKLKRKVAVVVP 180

Query: 181 KKDKDDGDGGDKNKSGGEVAQQDGGGETDGDRLDYMAVPAPGYEYGF--GYGYVYGHGGF 240
           KKD DDGDGGDKNKSGGEV Q+DGGGETDGDRLDYMAVPAPGYEYGF  GYGY YGHGGF
Sbjct: 181 KKDNDDGDGGDKNKSGGEVTQEDGGGETDGDRLDYMAVPAPGYEYGFGYGYGYGYGHGGF 240

Query: 241 VGEHLPSSQMFSDENPNACTVM 257
           VGEHLPSSQMFSDENPNACTVM
Sbjct: 241 VGEHLPSSQMFSDENPNACTVM 262

BLAST of CmaCh20G005110 vs. ExPASy TrEMBL
Match: A0A1S3CP34 (heavy metal-associated isoprenylated plant protein 3-like OS=Cucumis melo OX=3656 GN=LOC103502993 PE=4 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 2.1e-98
Identity = 201/268 (75.00%), Postives = 221/268 (82.46%), Query Frame = 0

Query: 10  GSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKFDASSLR 69
           G  K +D TTAVF ID+HCEGCANKLR+ VR+I GV RVRADWEANKLTVIGKFDA+ LR
Sbjct: 2   GEKKNDDITTAVFKIDIHCEGCANKLRKSVREIPGVARVRADWEANKLTVIGKFDAAKLR 61

Query: 70  EKLADKTKKKIDIVSLEGKKEKGS-KKPEDEK----------PKDEEAPATTATLKVELH 129
           + LADKTKKKIDIVS EGKKEK S KK +DEK          PKD+E P TTATLKVELH
Sbjct: 62  DNLADKTKKKIDIVSSEGKKEKESAKKQDDEKPDKKTEDKKQPKDKEIPVTTATLKVELH 121

Query: 130 CQGCIERIYKVVSRTKGVEDMAMETQKDLVTVKGKMDVKALIHNLEEKLKRKVAVVVPKK 189
           CQGCIE+IYKVVSRTKGVEDMA+E QKDLV VKGKMDVKALI NLEEKLKRKVAVVVPKK
Sbjct: 122 CQGCIEKIYKVVSRTKGVEDMAIERQKDLVMVKGKMDVKALIENLEEKLKRKVAVVVPKK 181

Query: 190 DKDD----GDGGDKNKSGGEVAQQDGGGETDGDRLDYMAVPAPGYEYGFGYGYVYGHGGF 249
           DKDD    GDGGDKNK+GGEVAQ+  GG  +G+RLDY+AVP PGY YG+GYGY YG+GGF
Sbjct: 182 DKDDGAKGGDGGDKNKTGGEVAQE--GGAMEGNRLDYVAVPVPGYGYGYGYGYGYGNGGF 241

Query: 250 VGEHLP------SSQMFSDENPNACTVM 257
           VG+HLP      S QMFSDENPNAC++M
Sbjct: 242 VGQHLPQPQPLLSPQMFSDENPNACSLM 267

BLAST of CmaCh20G005110 vs. ExPASy TrEMBL
Match: A0A0A0KDD7 (HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G147620 PE=4 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 1.5e-96
Identity = 199/268 (74.25%), Postives = 218/268 (81.34%), Query Frame = 0

Query: 10  GSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKFDASSLR 69
           G  K +D+TTAVF ID+HCEGCANKLRR VRQI GV RVRADWEANKLTVIGKFD + LR
Sbjct: 2   GEKKNDDTTTAVFKIDIHCEGCANKLRRSVRQIPGVSRVRADWEANKLTVIGKFDPAKLR 61

Query: 70  EKLADKTKKKIDIVSLEGKKEKGS-KKPEDEK----------PKDEEAPATTATLKVELH 129
           + LADK  KKIDIVS E KKEK S KK +DEK          PKD+E P TTATLKVELH
Sbjct: 62  DYLADKETKKIDIVSSESKKEKESTKKQDDEKPDKKTEDKKQPKDKEIPVTTATLKVELH 121

Query: 130 CQGCIERIYKVVSRTKGVEDMAMETQKDLVTVKGKMDVKALIHNLEEKLKRKVAVVVPKK 189
           CQGCIE+IYKVVSRTKGVEDMA+E QKDLV VKGKMDVKALI NLEEKLKRKVAVVVPKK
Sbjct: 122 CQGCIEKIYKVVSRTKGVEDMAIERQKDLVMVKGKMDVKALIENLEEKLKRKVAVVVPKK 181

Query: 190 DKDD----GDGGDKNKSGGEVAQQDGGGETDGDRLDYMAVPAPGYEYGFGYGYVYGHGGF 249
           DKD+    GDGGDKNK+GGEVAQ  GGG  +G+RLDY+AVP PGY YG+GYGY YG+GGF
Sbjct: 182 DKDEGAKGGDGGDKNKTGGEVAQ--GGGAMEGNRLDYVAVPVPGYGYGYGYGYGYGNGGF 241

Query: 250 VGEHLP------SSQMFSDENPNACTVM 257
           VG+H+P      S QMFSDENPNAC+VM
Sbjct: 242 VGQHMPPAQPLISPQMFSDENPNACSVM 267

BLAST of CmaCh20G005110 vs. ExPASy TrEMBL
Match: A0A6J1CGH5 (heavy metal-associated isoprenylated plant protein 3-like OS=Momordica charantia OX=3673 GN=LOC111011319 PE=4 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 4.8e-95
Identity = 195/268 (72.76%), Postives = 221/268 (82.46%), Query Frame = 0

Query: 1   MGKKKNGSHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVI 60
           MG KKNG  G  K+EDS++ VF ID+HCEGCANK+R+CVR+I+GVE+V+A+WE NKLTVI
Sbjct: 1   MGAKKNGDDGK-KKEDSSSVVFKIDIHCEGCANKIRKCVREIEGVEKVKAEWEINKLTVI 60

Query: 61  GKFDASSLREKLADKTKKKIDIV--SLEGKKEKG----SKKPEDEKPKDEEAPATTATLK 120
           GKFDAS LREKLADKTKKKIDIV  S E KKEK      KK E++KPK++E P TTATLK
Sbjct: 61  GKFDASKLREKLADKTKKKIDIVSSSSEKKKEKDEKAEDKKAEEKKPKEKETPVTTATLK 120

Query: 121 VELHCQGCIERIYKVVSRTKGVEDMAMETQKDLVTVKGKMDVKALIHNLEEKLKRKVAVV 180
           VELHCQGCI++IYKVVSRTKGVEDMA+E QKDLV VKGKMDVKALI NLEEKLKRKVAVV
Sbjct: 121 VELHCQGCIQKIYKVVSRTKGVEDMAIERQKDLVMVKGKMDVKALIQNLEEKLKRKVAVV 180

Query: 181 VPKKDKDD----GDGGDKNKSGGEVAQQ--DGGGETDGDRLDYMAVPAPGYEYGFGYGYV 240
           VPKKDKDD    GD  D NK+ G+ AQQ   GGG T+G+R DY AVP  GY YG+GYG  
Sbjct: 181 VPKKDKDDGAKGGDAADNNKTAGDAAQQPTTGGGITEGNRPDYAAVPVQGYGYGYGYG-- 240

Query: 241 YGHGGFVGEHLPSSQMFSDENPNACTVM 257
           YG GG+VG+HLPS QMFSDENPNAC+VM
Sbjct: 241 YGPGGYVGQHLPSPQMFSDENPNACSVM 265

BLAST of CmaCh20G005110 vs. NCBI nr
Match: XP_022985576.1 (heavy metal-associated isoprenylated plant protein 3 [Cucurbita maxima])

HSP 1 Score: 503.8 bits (1296), Expect = 8.6e-139
Identity = 256/256 (100.00%), Postives = 256/256 (100.00%), Query Frame = 0

Query: 1   MGKKKNGSHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVI 60
           MGKKKNGSHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVI
Sbjct: 1   MGKKKNGSHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVI 60

Query: 61  GKFDASSLREKLADKTKKKIDIVSLEGKKEKGSKKPEDEKPKDEEAPATTATLKVELHCQ 120
           GKFDASSLREKLADKTKKKIDIVSLEGKKEKGSKKPEDEKPKDEEAPATTATLKVELHCQ
Sbjct: 61  GKFDASSLREKLADKTKKKIDIVSLEGKKEKGSKKPEDEKPKDEEAPATTATLKVELHCQ 120

Query: 121 GCIERIYKVVSRTKGVEDMAMETQKDLVTVKGKMDVKALIHNLEEKLKRKVAVVVPKKDK 180
           GCIERIYKVVSRTKGVEDMAMETQKDLVTVKGKMDVKALIHNLEEKLKRKVAVVVPKKDK
Sbjct: 121 GCIERIYKVVSRTKGVEDMAMETQKDLVTVKGKMDVKALIHNLEEKLKRKVAVVVPKKDK 180

Query: 181 DDGDGGDKNKSGGEVAQQDGGGETDGDRLDYMAVPAPGYEYGFGYGYVYGHGGFVGEHLP 240
           DDGDGGDKNKSGGEVAQQDGGGETDGDRLDYMAVPAPGYEYGFGYGYVYGHGGFVGEHLP
Sbjct: 181 DDGDGGDKNKSGGEVAQQDGGGETDGDRLDYMAVPAPGYEYGFGYGYVYGHGGFVGEHLP 240

Query: 241 SSQMFSDENPNACTVM 257
           SSQMFSDENPNACTVM
Sbjct: 241 SSQMFSDENPNACTVM 256

BLAST of CmaCh20G005110 vs. NCBI nr
Match: XP_023513163.1 (heavy metal-associated isoprenylated plant protein 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 474.9 bits (1221), Expect = 4.3e-130
Identity = 240/256 (93.75%), Postives = 247/256 (96.48%), Query Frame = 0

Query: 1   MGKKKNGSHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVI 60
           MGKK+NG+HGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVI
Sbjct: 1   MGKKQNGAHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVI 60

Query: 61  GKFDASSLREKLADKTKKKIDIVSLEGKKEKGSKKPEDEKPKDEEAPATTATLKVELHCQ 120
           GKFDAS+LREKLA KTKKKIDIVS EGKKEK SKKPEDEKPKDEEAP TTATLKVELHCQ
Sbjct: 61  GKFDASTLREKLAGKTKKKIDIVSTEGKKEKKSKKPEDEKPKDEEAPVTTATLKVELHCQ 120

Query: 121 GCIERIYKVVSRTKGVEDMAMETQKDLVTVKGKMDVKALIHNLEEKLKRKVAVVVPKKDK 180
           GC+ERIYKVVSRTKGVEDMAME Q DLVTVKGKMDVKALIHNLE+KLKRKVAVVVPKKDK
Sbjct: 121 GCVERIYKVVSRTKGVEDMAMERQMDLVTVKGKMDVKALIHNLEDKLKRKVAVVVPKKDK 180

Query: 181 DDGDGGDKNKSGGEVAQQDGGGETDGDRLDYMAVPAPGYEYGFGYGYVYGHGGFVGEHLP 240
           DDGDG DKNKSGGEV Q++GGGETDGDRLDYMAVPAPGYEYGFGYGY YGHGGFVGEHLP
Sbjct: 181 DDGDGADKNKSGGEVTQENGGGETDGDRLDYMAVPAPGYEYGFGYGYGYGHGGFVGEHLP 240

Query: 241 SSQMFSDENPNACTVM 257
           SSQMFSDENPNACTVM
Sbjct: 241 SSQMFSDENPNACTVM 256

BLAST of CmaCh20G005110 vs. NCBI nr
Match: XP_022944012.1 (heavy metal-associated isoprenylated plant protein 3 [Cucurbita moschata])

HSP 1 Score: 466.5 bits (1199), Expect = 1.5e-127
Identity = 240/262 (91.60%), Postives = 247/262 (94.27%), Query Frame = 0

Query: 1   MGKKKNGSHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVI 60
           MGKK+NGSHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVI
Sbjct: 1   MGKKQNGSHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVI 60

Query: 61  GKFDASSLREKLADKTKKKIDIVSLEGKKEKGSKKP----EDEKPKDEEAPATTATLKVE 120
           GKFDAS+LREKLADKT KKIDIVS EGKKEK SKKP    EDEKPKD+E P TTATLKVE
Sbjct: 61  GKFDASTLREKLADKTNKKIDIVSSEGKKEKKSKKPDEEAEDEKPKDKEIPVTTATLKVE 120

Query: 121 LHCQGCIERIYKVVSRTKGVEDMAMETQKDLVTVKGKMDVKALIHNLEEKLKRKVAVVVP 180
           LHCQGC+ERIYKVVSRTKGVEDMAME QKDLVTVKGKMDVKAL+HNLE+KLKRKVAVVVP
Sbjct: 121 LHCQGCVERIYKVVSRTKGVEDMAMERQKDLVTVKGKMDVKALVHNLEDKLKRKVAVVVP 180

Query: 181 KKDKDDGDGGDKNKSGGEVAQQDGGGETDGDRLDYMAVPAPGYEYGF--GYGYVYGHGGF 240
           KKD DDGDGGDKNKSGGEV Q+DGGGETDGDRLDYMAVPAPGYEYGF  GYGY YGHGGF
Sbjct: 181 KKDNDDGDGGDKNKSGGEVTQEDGGGETDGDRLDYMAVPAPGYEYGFGYGYGYGYGHGGF 240

Query: 241 VGEHLPSSQMFSDENPNACTVM 257
           VGEHLPSSQMFSDENPNACTVM
Sbjct: 241 VGEHLPSSQMFSDENPNACTVM 262

BLAST of CmaCh20G005110 vs. NCBI nr
Match: KAG7010652.1 (Heavy metal-associated isoprenylated plant protein 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 454.1 bits (1167), Expect = 7.8e-124
Identity = 233/259 (89.96%), Postives = 243/259 (93.82%), Query Frame = 0

Query: 4   KKNGSHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKF 63
           K+N  HGSDK+EDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVR DWEANKLTVIGKF
Sbjct: 1   KQNSGHGSDKQEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRVDWEANKLTVIGKF 60

Query: 64  DASSLREKLADKTKKKIDIVSLEGKKEKGSKKP----EDEKPKDEEAPATTATLKVELHC 123
           DAS+LREKLADKTKKKIDIVS EGKKEK SKKP    EDEKPKD+E P TTATLKVELHC
Sbjct: 61  DASTLREKLADKTKKKIDIVSSEGKKEKKSKKPDEEAEDEKPKDKEIPVTTATLKVELHC 120

Query: 124 QGCIERIYKVVSRTKGVEDMAMETQKDLVTVKGKMDVKALIHNLEEKLKRKVAVVVPKKD 183
           QGC+ERIYKVVSRTKGVEDMAME QKDLVTVKGKMDVKAL+HNLE+KLKRKVAVVVPKKD
Sbjct: 121 QGCVERIYKVVSRTKGVEDMAMERQKDLVTVKGKMDVKALVHNLEDKLKRKVAVVVPKKD 180

Query: 184 KDDGDGGDKNKSGGEVAQQDGGGETDGDRLDYMAVPAPGYE--YGFGYGYVYGHGGFVGE 243
            DDGDGGDKNKSGGEVAQ++GGGETDGDRLDYMAVPAPGYE  YG+GYGY YGHGGFVGE
Sbjct: 181 SDDGDGGDKNKSGGEVAQENGGGETDGDRLDYMAVPAPGYEYGYGYGYGYGYGHGGFVGE 240

Query: 244 HLPSSQMFSDENPNACTVM 257
           HLPSSQMFSDENPNACTVM
Sbjct: 241 HLPSSQMFSDENPNACTVM 259

BLAST of CmaCh20G005110 vs. NCBI nr
Match: KAG6570811.1 (Heavy metal-associated isoprenylated plant protein 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 382.1 bits (980), Expect = 3.8e-102
Identity = 199/220 (90.45%), Postives = 206/220 (93.64%), Query Frame = 0

Query: 43  QGVERVRADWEANKLTVIGKFDASSLREKLADKTKKKIDIVSLEGKKEKGSKKP----ED 102
           +GVERVRADWEANKLTVIGKFDAS+LREKLADKTKKKIDIVS EGKKEK SKKP    ED
Sbjct: 96  EGVERVRADWEANKLTVIGKFDASTLREKLADKTKKKIDIVSSEGKKEKKSKKPDEEAED 155

Query: 103 EKPKDEEAPATTATLKVELHCQGCIERIYKVVSRTKGVEDMAMETQKDLVTVKGKMDVKA 162
           EKPKD+E P TTATLKVELHCQGC+ERIYKVVSRTKGVEDMAME QKDLVTVKGKMDVKA
Sbjct: 156 EKPKDKEIPVTTATLKVELHCQGCVERIYKVVSRTKGVEDMAMERQKDLVTVKGKMDVKA 215

Query: 163 LIHNLEEKLKRKVAVVVPKKDKDDGDGGDKNKSGGEVAQQDGGGETDGDRLDYMAVPAPG 222
           L+HNLE+KLKRKVAVVVPKKD DDGDGGDKNKSGGEV Q+DGGGETDGDRLDYMAVPAPG
Sbjct: 216 LVHNLEDKLKRKVAVVVPKKDSDDGDGGDKNKSGGEVTQEDGGGETDGDRLDYMAVPAPG 275

Query: 223 YEYGF--GYGYVYGHGGFVGEHLPSSQMFSDENPNACTVM 257
           YEYGF  GYGY YGHGGFVGEHLPSSQMFSDENPNACTVM
Sbjct: 276 YEYGFGYGYGYGYGHGGFVGEHLPSSQMFSDENPNACTVM 315

BLAST of CmaCh20G005110 vs. TAIR 10
Match: AT5G60800.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 209.5 bits (532), Expect = 3.1e-54
Identity = 131/279 (46.95%), Postives = 168/279 (60.22%), Query Frame = 0

Query: 3   KKKNGSHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGK 62
           KKK G +    E  S T V  +DMHCEGCA+++ +CVR  QGVE V+++    KLTV G 
Sbjct: 11  KKKGGDNKKKNETPSITVVLKVDMHCEGCASRIVKCVRSFQGVETVKSESATGKLTVTGA 70

Query: 63  FDASSLREKLADKTKKKIDIVSLEGKKEK----------------GSKKPEDEKPKDEEA 122
            D   LREKL +KTKKK+D+VS + KKEK                  KKP++   K +E 
Sbjct: 71  LDPVKLREKLEEKTKKKVDLVSPQPKKEKEKENKNKNDEDKKKSEEKKKPDNNDKKPKET 130

Query: 123 PATTATLKVELHCQGCIERIYKVVSRTKGVEDMAMETQKDLVTVKGKMDVKALIHNLEEK 182
           P TTA LK+  HCQGCI +I K V++TKGV  + M+ +K+L+TVKG MDVK L+  L EK
Sbjct: 131 PVTTAVLKLNFHCQGCIGKIQKTVTKTKGVNGLTMDKEKNLLTVKGTMDVKKLVEILSEK 190

Query: 183 LKRKVAVVVPKKDKDDGDG---GDKNKSGG------EVAQQDGGGETDGDRLDYMAVPAP 242
           LKR V +V PKK+KD  +G   G+K K GG      E     GGGE   + ++YMA   P
Sbjct: 191 LKRAVEIVPPKKEKDKENGNENGEKKKGGGGDGGGKEKTGNKGGGE-GVNMMEYMAA-QP 250

Query: 243 GYEYGFGYGYVYGHGGFVGEHLPSSQMFSDENPNACTVM 257
            Y YG+  G  YG+   +  H P  Q+FSDENPNAC VM
Sbjct: 251 AYGYGYYPGGPYGYP--IQAHAP--QIFSDENPNACVVM 283

BLAST of CmaCh20G005110 vs. TAIR 10
Match: AT5G60800.2 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 208.0 bits (528), Expect = 9.1e-54
Identity = 130/279 (46.59%), Postives = 168/279 (60.22%), Query Frame = 0

Query: 3   KKKNGSHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGK 62
           KKK G +    E  S T V  +DMHCEGCA+++ +CVR  QGVE V+++    KLTV G 
Sbjct: 11  KKKGGDNKKKNETPSITVVLKVDMHCEGCASRIVKCVRSFQGVETVKSESATGKLTVTGA 70

Query: 63  FDASSLREKLADKTKKKIDIVSLEGKKEK----------------GSKKPEDEKPKDEEA 122
            D   LREKL +KTKKK+D+VS + KKEK                  KKP++   K +E 
Sbjct: 71  LDPVKLREKLEEKTKKKVDLVSPQPKKEKEKENKNKNDEDKKKSEEKKKPDNNDKKPKET 130

Query: 123 PATTATLKVELHCQGCIERIYKVVSRTKGVEDMAMETQKDLVTVKGKMDVKALIHNLEEK 182
           P TTA LK+  HCQGCI +I K V++TKGV  + M+ +K+L+TVKG MDVK L+  L EK
Sbjct: 131 PVTTAVLKLNFHCQGCIGKIQKTVTKTKGVNGLTMDKEKNLLTVKGTMDVKKLVEILSEK 190

Query: 183 LKRKVAVVVPKKDKDDGDG---GDKNKSGG------EVAQQDGGGETDGDRLDYMAVPAP 242
           LKR V +V PKK+KD  +G   G+K K GG      E     GGGE   + ++YMA   P
Sbjct: 191 LKRAVEIVPPKKEKDKENGNENGEKKKGGGGDGGGKEKTGNKGGGE-GVNMMEYMAA-QP 250

Query: 243 GYEYGFGYGYVYGHGGFVGEHLPSSQMFSDENPNACTVM 257
            Y YG+  G  YG+   +  H P  Q+FSDENPNAC V+
Sbjct: 251 AYGYGYYPGGPYGYP--IQAHAP--QIFSDENPNACVVI 283

BLAST of CmaCh20G005110 vs. TAIR 10
Match: AT2G36950.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 137.1 bits (344), Expect = 2.0e-32
Identity = 118/359 (32.87%), Postives = 161/359 (44.85%), Query Frame = 0

Query: 2   GKKKNGSHGSDKEEDSTTA------VFNIDMHCEGCANKLRRCVRQIQGVERVRADWEAN 61
           GK K+G   S        A      V+ +DMHCEGCA K++R V+   GV+ V AD   N
Sbjct: 28  GKPKSGGGDSAAAAAPPVAAVVSAFVYKVDMHCEGCAKKIKRMVKHFDGVKDVTADTGGN 87

Query: 62  KLTVIGKFDASSLREKLADKTKKKIDIVS----LEG---------KKEKGSKKPEDEKPK 121
           KL V+GK D   L+EKL +KTK+K+ + +    +EG         K + G K+     P 
Sbjct: 88  KLLVVGKIDPVKLQEKLEEKTKRKVVLANPPPKVEGPVAAAVGEKKADGGDKEAAPPAPA 147

Query: 122 DEEAPATTATLKVELHCQGCIERIYKVVSRTKGVEDMAMETQKDLVTVKGKMDVKALIHN 181
                 +   LK+ LHC+GCI++I K++ + KGVE +A++  KD+VTVKG +DVK L+  
Sbjct: 148 PAAPKESVVPLKIRLHCEGCIQKIKKIILKIKGVETVAIDGAKDVVTVKGTIDVKELVPL 207

Query: 182 LEEKLKRKVAVVVP----------------------------------------KKDKDD 241
           L +KLKR V  +VP                                        +K K+ 
Sbjct: 208 LTKKLKRTVEPLVPAKKDDGAAENKKTEAAAPDAKKEAPSAGVNEAKKEGSDGGEKKKEV 267

Query: 242 GDGGDKNKSGGEVAQ------------QDGGG-------ETDGDRLDYMAVP-AP----- 257
           GDGG+K K GG+  +            +DGGG           D   Y A P AP     
Sbjct: 268 GDGGEKKKEGGDGGEKKKEAGDGGEKKKDGGGVPAPVAMVNKMDYYGYSAYPTAPMHWQE 327

BLAST of CmaCh20G005110 vs. TAIR 10
Match: AT5G03380.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 132.9 bits (333), Expect = 3.7e-31
Identity = 122/392 (31.12%), Postives = 169/392 (43.11%), Query Frame = 0

Query: 1   MGKKKNGS----HGSDKEEDS--TTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEA 60
           MG+KK  +     G  K  D   TT V  +DMHCEGC  K++R  +  +GVE V+ D+++
Sbjct: 1   MGEKKEETATKPQGEKKPTDGGITTVVMKLDMHCEGCGKKIKRIFKHFKGVEDVKIDYKS 60

Query: 61  NKLTVIGKFDASSLREKLADKTKKKIDIVSL----------------------------- 120
           NKLTVIG  D   +R+K+ADK K+ +++VS                              
Sbjct: 61  NKLTVIGNVDPVEVRDKVADKIKRPVELVSTVAPPKKETPPSSGGAEKKPSPAAEEKPAE 120

Query: 121 --------EGKKEKGSKKPEDEKPKDEEAP--ATTATLKVELHCQGCIERIYKVVSRTKG 180
                    G+K++  KK E EK      P   +T  LK +LHC+GC  +I ++V++ KG
Sbjct: 121 KKPAAVEKPGEKKEEKKKEEGEKKASPPPPPKESTVVLKTKLHCEGCEHKIKRIVNKIKG 180

Query: 181 VEDMAMETQKDLVTVKGKMDVKALIHNLEEKLKRKVAVVVPKKD---------------- 240
           V  +A+++ KDLV VKG +DVK L   L EKLKR V VV  KKD                
Sbjct: 181 VNSVAIDSAKDLVIVKGIIDVKQLTPYLNEKLKRTVEVVPAKKDDGAPVAAAAAAPAGGE 240

Query: 241 ---------KDDGDGGDKNKSGGEVAQQD---------GGGETDGDRLD--------YMA 257
                    K+  D G+K   GG   +++         GGG  DG  +D        Y  
Sbjct: 241 KKDKVAGEKKEIKDVGEKKVDGGGEKKKEVAVGGGGGGGGGGGDGGAMDVKKSEYNGYGY 300

BLAST of CmaCh20G005110 vs. TAIR 10
Match: AT2G28090.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 131.3 bits (329), Expect = 1.1e-30
Identity = 95/254 (37.40%), Postives = 141/254 (55.51%), Query Frame = 0

Query: 13  KEEDSTTA----VFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIG-KFDASS 72
           K++++TT+    V  ID HC+GC  ++ R  R+++GVE VRAD ++NKLT+IG   D   
Sbjct: 20  KKQNNTTSPVHVVLKIDFHCDGCIARIVRLSRRLEGVETVRADPDSNKLTLIGFIMDPVK 79

Query: 73  LREKLADKTKKKIDIVSLEGKKEKGSKKPEDEKPKDEE---APATTATLKVELHCQGCIE 132
           + EKL  K+KKK++++S + KK+  +K+  ++K  D+       TT  LKV   C GCI+
Sbjct: 80  IAEKLQKKSKKKVELISPKPKKD--TKENNEKKANDKTQTVVAVTTVVLKVNCSCDGCIK 139

Query: 133 RIYKVVSRTKGVEDMAMETQKDLVTVKGKMDVKALIHNLEEKLKRKVAVV-VPKKDKDDG 192
           RI K VS TKGV  + M+ +K+ VTV G MD+K++  NL+ KLK+ V VV   KK K D 
Sbjct: 140 RIQKAVSTTKGVYQVKMDKEKETVTVMGTMDIKSVTDNLKRKLKKTVQVVPEKKKKKKDK 199

Query: 193 DGGDKNKSGGEVAQQDGGGETDGDRLDYMAVPAPGYEYGFG-YGYVYGHGGFVGEHLPSS 252
           D  + N   G   Q                 P  G  +G G Y ++ G         P +
Sbjct: 200 DNAEVNTKVGSPCQ-----------------PGNGCTHGIGPYRFMEG---------PMT 245

Query: 253 QMFSDENPNACTVM 257
             FS+E+ + C+VM
Sbjct: 260 GFFSEEDQSYCSVM 245

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FJH54.4e-5346.95Heavy metal-associated isoprenylated plant protein 3 OS=Arabidopsis thaliana OX=... [more]
Q9SJL22.8e-3132.87Heavy metal-associated isoprenylated plant protein 5 OS=Arabidopsis thaliana OX=... [more]
Q9LZF15.2e-3031.12Heavy metal-associated isoprenylated plant protein 6 OS=Arabidopsis thaliana OX=... [more]
Q9ZUV11.5e-2937.40Heavy metal-associated isoprenylated plant protein 1 OS=Arabidopsis thaliana OX=... [more]
Q9FLU53.2e-1928.90Heavy metal-associated isoprenylated plant protein 9 OS=Arabidopsis thaliana OX=... [more]
Match NameE-valueIdentityDescription
A0A6J1J8L74.1e-139100.00heavy metal-associated isoprenylated plant protein 3 OS=Cucurbita maxima OX=3661... [more]
A0A6J1FUL57.3e-12891.60heavy metal-associated isoprenylated plant protein 3 OS=Cucurbita moschata OX=36... [more]
A0A1S3CP342.1e-9875.00heavy metal-associated isoprenylated plant protein 3-like OS=Cucumis melo OX=365... [more]
A0A0A0KDD71.5e-9674.25HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G147620 PE=4 SV... [more]
A0A6J1CGH54.8e-9572.76heavy metal-associated isoprenylated plant protein 3-like OS=Momordica charantia... [more]
Match NameE-valueIdentityDescription
XP_022985576.18.6e-139100.00heavy metal-associated isoprenylated plant protein 3 [Cucurbita maxima][more]
XP_023513163.14.3e-13093.75heavy metal-associated isoprenylated plant protein 3 [Cucurbita pepo subsp. pepo... [more]
XP_022944012.11.5e-12791.60heavy metal-associated isoprenylated plant protein 3 [Cucurbita moschata][more]
KAG7010652.17.8e-12489.96Heavy metal-associated isoprenylated plant protein 3, partial [Cucurbita argyros... [more]
KAG6570811.13.8e-10290.45Heavy metal-associated isoprenylated plant protein 3, partial [Cucurbita argyros... [more]
Match NameE-valueIdentityDescription
AT5G60800.13.1e-5446.95Heavy metal transport/detoxification superfamily protein [more]
AT5G60800.29.1e-5446.59Heavy metal transport/detoxification superfamily protein [more]
AT2G36950.12.0e-3232.87Heavy metal transport/detoxification superfamily protein [more]
AT5G03380.13.7e-3131.12Heavy metal transport/detoxification superfamily protein [more]
AT2G28090.11.1e-3037.40Heavy metal transport/detoxification superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006121Heavy metal-associated domain, HMAPFAMPF00403HMAcoord: 21..75
e-value: 3.1E-9
score: 37.1
coord: 112..169
e-value: 4.7E-9
score: 36.5
IPR006121Heavy metal-associated domain, HMAPROSITEPS50846HMA_2coord: 26..59
score: 9.167534
IPR006121Heavy metal-associated domain, HMACDDcd00371HMAcoord: 26..78
e-value: 2.86263E-7
score: 44.5189
IPR006121Heavy metal-associated domain, HMACDDcd00371HMAcoord: 112..165
e-value: 4.66777E-4
score: 35.6593
NoneNo IPR availableGENE3D3.30.70.100coord: 17..91
e-value: 6.3E-16
score: 60.2
coord: 108..181
e-value: 5.2E-14
score: 54.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 174..189
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 174..209
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 87..109
NoneNo IPR availablePANTHERPTHR46413:SF2HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 3coord: 1..256
IPR044594Heavy metal-associated isoprenylated plant protein 1/3/5/6PANTHERPTHR46413HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 6coord: 1..256
IPR036163Heavy metal-associated domain superfamilySUPERFAMILY55008HMA, heavy metal-associated domaincoord: 16..74
IPR036163Heavy metal-associated domain superfamilySUPERFAMILY55008HMA, heavy metal-associated domaincoord: 108..165

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh20G005110.1CmaCh20G005110.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding