CmaCh20G000140 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh20G000140
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionNADH-cytochrome b5 reductase
LocationCma_Chr20: 36360 .. 39041 (+)
RNA-Seq ExpressionCmaCh20G000140
SyntenyCmaCh20G000140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGGGTTGTGAAAGGTGTATCGTGAATGGTTTATTCCAAAACTAAAAGGTTGACAATCCATTATTTATTCCCTTCTTTTTATATATTTCGAAGCTACGGACGGATGTGGGGGGGATGAGATCCCATTTTCGGCTGCATCCGCATCTCCCAACAACCGCAACCATTGAAGCCCTGTGATCCGATTCGTGCTCTTCCTGCAGATGGCGACGAGCTTCGTCCACTCTCACAGACCCGAGTTTCTCACCGTCGCCGTCGCTGCGGTTGCCATCGGCGTTGCTACTACCTACTATTTCTACATCACCAAGAAATCTAAACCCCAAGGTTCTTATTCCTTCTCTTCCCTAATTCCTTCTACTCGGGCATTTTTGTTGTTTTGCTTGATTAAGATGCCGGGCTCCGGCTGTTGGATTTGGGAATACCTAGAACAAACCCTCCCCATCCCTATTTCATTTTTGCTGCTGATATCATCACTACCCTGCTGTTCGAGATGTTTCCGATTTTTACCGGGTTTTCTTCTTCTCTTTCTTATCTGTAGTTTCGTGTTATGCTAGGCTGCTTGGACCCTGATAAATTCAAAGAATTTAAGCTAGTTAAACGCACACAGCTTAGCCATAATGTAGCTAAGTTCAGATTCTCTCTTCCGACGCCAACCTCTATCTTGGGGCTGCCGATTGGGCAGCATATGAGTTGCAGGTTTTATTCGATTTGATACTTCGTGGAGTAAAGATTTATCAATGGAATGTTATGAGTCTTCAAACGATTTGTTGTTGTAAGTTATGATGTGCTTTTGTTCAGAGGAAAAGATAGCGAAGGTGAAGAAGTTGTGAAACCGTACACTCCAACCAGTTTGGATACAGATGTTGGATACTTTGATTTGGTCATAAAGGTATAGGCCTTGCACTCCCAATGGGCAATAGCTTGCAGTTTTTCTTAGTGTGTTTAACCATCTGCCCAATTCTATCCATCCAACCATAACATTTGGTATTTTGGCATACAACTACTACAATATGTAGATGTATCCTCAAGGAAGGATGTCCCACCACTTTCGTGAGATGCGTGAAGGTGATTTCTTGGCGGTGAAAGGTCCTAAGGTATTGCCAATATGTCCTACAACTGCCACTACATTAGTAGAATTGAGATTTTCATTTTTTACTCTGTTAATTTGAACCTTGAACGGTTAACTTGGTCAGAGTGAATCTTAGGCTTGAGTATACCTTTTATTTTGTCAAGTGTTCGCATTCTTTTTGAAAGGTTTATGAGTGGTTATGAGTATTCGTATGGCTGGACTGGGTTTGAATATTTTGTGGCTGCTAGTTACTAAGATGGCGTTGGATGCTTTTTAGTTGTGTTTAAGCAGTCTATTATTGAGTTTGAGGAGTTATTCTCTTTTGTTTGAATAGGTACGTTTACACAATTTTTTGGCTCGATTTTGGTTCTTTATTTGTTACTCTTGGGTGATTTATGGCTGGTTGAGTAATGTTTATTATTTATGTATATCGTTTGAGGAGGCATTGTTTTAACCACTCATCAAGTTTTAGGGTTTGAATTGGACGGAATAAAAAATTGAAGGCAAAGATTGACCGTTAAGGAAAAAATCAGTCCTTTTGGGGGATTGGGATTCATAATAAAACATAAGGTTATATTAGAACTTCGAGGAGCGCATTGAACAGCCCAACTGCTTCTGGTCTCAAATTTTCTGTTTTGGAGGAGCTAGGTGCTTAAAATATTTGATTTTTGACATTTGATGTGGGAAAATCTAAAACTGGGGCACGGGTTCTGGGGTCATATCTATTATTTATAGAGTGGGTCTCTGTTTGTGTGCGCGAGTGGTGACAGGGACGTTTCAAGTATGAAGCTAATGAAGTTGAGGCCTTTGGGATGCTTGCTGGAGGCACAGGCATAACTCCTATGTTCCAGGTATGGGTATGGGTATGGGTATGGGCATGGGCTAAGTTTGATCTTAAGTTTTGAGTAAGGATTGATTCGTATTTGTTTTCTGTTTGTCCTATAAATAAGCAGATCCCATCAACATTTCTGAACGTTTCTTTTTGTGTTTTGAGGTTGCACGAGCAATACTAGAAAACCCAGTTGATCACACCAATGCGCATCTTATATATGCTAATGCTACTTATGAAGACATTCTTCTAAAGGTGCTCATTCTCCTCTCTTAAATTTGGATTTGGAAGGTACCCCACATTGAGATTTAGATATTGCCTTGCAAAACCGAACTGAAACCTTCCAGTTTACTGGTGTTTATAGGAAGAATTGGATAGTCTTGCGTCTAGGTTCCCTTCCCGCTTTTCTGTCTACTATGTGCTCGATCAGGTAACAACTTGGGGTTTTATAATTTTGGAAATATATTCTGCAGTTCCATGTCGTTTTACAATCTCCCTCTGCTCTGTACAGCCTCCTGAAGGATGGGATGGCGGCGTTGGCTTTGTGACTGAGGAAATGATACGACTACACTGTCCTGGACCATCCCCTAATGTTCGGGTGGGTATGCATGCGTTGCTTCATTCGTTTTTCTTTCTCTCTGTAAGAGAAAGTTTGTGCTAATAGCAGGCATTATTGTTGTTCATTTTGCGACAGATATTGAGGTGCGGACCACCCCCCATGAACAAAGCTATGGGTGCTTACCTCAAAACTATTGGCTATACTTACCAAATGCAGTTCCAGTTCTAA

mRNA sequence

GTGGGTTGTGAAAGGTGTATCGTGAATGGTTTATTCCAAAACTAAAAGGTTGACAATCCATTATTTATTCCCTTCTTTTTATATATTTCGAAGCTACGGACGGATGTGGGGGGGATGAGATCCCATTTTCGGCTGCATCCGCATCTCCCAACAACCGCAACCATTGAAGCCCTGTGATCCGATTCGTGCTCTTCCTGCAGATGGCGACGAGCTTCGTCCACTCTCACAGACCCGAGTTTCTCACCGTCGCCGTCGCTGCGGTTGCCATCGGCGTTGCTACTACCTACTATTTCTACATCACCAAGAAATCTAAACCCCAAGGCTGCTTGGACCCTGATAAATTCAAAGAATTTAAGCTAGTTAAACGCACACAGCTTAGCCATAATGTAGCTAAGTTCAGATTCTCTCTTCCGACGCCAACCTCTATCTTGGGGCTGCCGATTGGGCAGCATATGAGTTGCAGAGGAAAAGATAGCGAAGGTGAAGAAGTTGTGAAACCGTACACTCCAACCAGTTTGGATACAGATGTTGGATACTTTGATTTGGTCATAAAGATGTATCCTCAAGGAAGGATGTCCCACCACTTTCGTGAGATGCGTGAAGGTGATTTCTTGGCGGTGAAAGGTCCTAAGGGACGTTTCAAGTATGAAGCTAATGAAGTTGAGGCCTTTGGGATGCTTGCTGGAGGCACAGGCATAACTCCTATGTTCCAGGTTGCACGAGCAATACTAGAAAACCCAGTTGATCACACCAATGCGCATCTTATATATGCTAATGCTACTTATGAAGACATTCTTCTAAAGGAAGAATTGGATAGTCTTGCGTCTAGGTTCCCTTCCCGCTTTTCTGTCTACTATGTGCTCGATCAGCCTCCTGAAGGATGGGATGGCGGCGTTGGCTTTGTGACTGAGGAAATGATACGACTACACTGTCCTGGACCATCCCCTAATGTTCGGATATTGAGGTGCGGACCACCCCCCATGAACAAAGCTATGGGTGCTTACCTCAAAACTATTGGCTATACTTACCAAATGCAGTTCCAGTTCTAA

Coding sequence (CDS)

ATGGCGACGAGCTTCGTCCACTCTCACAGACCCGAGTTTCTCACCGTCGCCGTCGCTGCGGTTGCCATCGGCGTTGCTACTACCTACTATTTCTACATCACCAAGAAATCTAAACCCCAAGGCTGCTTGGACCCTGATAAATTCAAAGAATTTAAGCTAGTTAAACGCACACAGCTTAGCCATAATGTAGCTAAGTTCAGATTCTCTCTTCCGACGCCAACCTCTATCTTGGGGCTGCCGATTGGGCAGCATATGAGTTGCAGAGGAAAAGATAGCGAAGGTGAAGAAGTTGTGAAACCGTACACTCCAACCAGTTTGGATACAGATGTTGGATACTTTGATTTGGTCATAAAGATGTATCCTCAAGGAAGGATGTCCCACCACTTTCGTGAGATGCGTGAAGGTGATTTCTTGGCGGTGAAAGGTCCTAAGGGACGTTTCAAGTATGAAGCTAATGAAGTTGAGGCCTTTGGGATGCTTGCTGGAGGCACAGGCATAACTCCTATGTTCCAGGTTGCACGAGCAATACTAGAAAACCCAGTTGATCACACCAATGCGCATCTTATATATGCTAATGCTACTTATGAAGACATTCTTCTAAAGGAAGAATTGGATAGTCTTGCGTCTAGGTTCCCTTCCCGCTTTTCTGTCTACTATGTGCTCGATCAGCCTCCTGAAGGATGGGATGGCGGCGTTGGCTTTGTGACTGAGGAAATGATACGACTACACTGTCCTGGACCATCCCCTAATGTTCGGATATTGAGGTGCGGACCACCCCCCATGAACAAAGCTATGGGTGCTTACCTCAAAACTATTGGCTATACTTACCAAATGCAGTTCCAGTTCTAA

Protein sequence

MATSFVHSHRPEFLTVAVAAVAIGVATTYYFYITKKSKPQGCLDPDKFKEFKLVKRTQLSHNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMYPQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENPVDHTNAHLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMIRLHCPGPSPNVRILRCGPPPMNKAMGAYLKTIGYTYQMQFQF
Homology
BLAST of CmaCh20G000140 vs. ExPASy Swiss-Prot
Match: Q9ZNT1 (NADH--cytochrome b5 reductase 1 OS=Arabidopsis thaliana OX=3702 GN=CBR1 PE=1 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 1.9e-113
Identity = 193/283 (68.20%), Postives = 230/283 (81.27%), Query Frame = 0

Query: 1   MATSFVHS-HRPEFLTVAVAAVAIGVATTYYFYITKKSKPQGCLDPDKFKEFKLVKRTQL 60
           M T F+ +  R   L V VA VA+G    Y  ++T   K + CLDP+ FKEFKLVKR QL
Sbjct: 1   MDTEFLRTLDRQILLGVFVAFVAVGAGAAY--FLTSSKKRRVCLDPENFKEFKLVKRHQL 60

Query: 61  SHNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKM 120
           SHNVAKF F LPT TS+LGLPIGQH+SCRGKD +GE+V+KPYTPT+LD+DVG F+LVIKM
Sbjct: 61  SHNVAKFVFELPTSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPTTLDSDVGRFELVIKM 120

Query: 121 YPQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILEN 180
           YPQGRMSHHFREMR GD LAVKGPKGRFKY+  +  AFGMLAGG+GITPMFQVARAILEN
Sbjct: 121 YPQGRMSHHFREMRVGDHLAVKGPKGRFKYQPGQFRAFGMLAGGSGITPMFQVARAILEN 180

Query: 181 PVDHTNAHLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEM 240
           P D T  HLIYAN TY+DILLKEEL+ L + +P +F ++YVL+QPPE WDGGVGFV++EM
Sbjct: 181 PTDKTKVHLIYANVTYDDILLKEELEGLTTNYPEQFKIFYVLNQPPEVWDGGVGFVSKEM 240

Query: 241 IRLHCPGPSPNVRILRCGPPPMNKAMGAYLKTIGYTYQMQFQF 283
           I+ HCP P+ +++ILRCGPPPMNKAM A L+ +GY+ +MQFQF
Sbjct: 241 IQTHCPAPASDIQILRCGPPPMNKAMAANLEALGYSPEMQFQF 281

BLAST of CmaCh20G000140 vs. ExPASy Swiss-Prot
Match: Q54NC1 (NADH-cytochrome b5 reductase 1 OS=Dictyostelium discoideum OX=44689 GN=cyb5r1 PE=3 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 7.4e-70
Identity = 134/284 (47.18%), Postives = 182/284 (64.08%), Query Frame = 0

Query: 12  EFLTVAVAAVAIGVATTYYFYIT-------------KKSKPQGCLDPDKFKEFKLVKRTQ 71
           +F+ V + +VA+     Y F +T             K  + +  LDP ++++F+L ++  
Sbjct: 5   DFILVIIGSVALAAGVKYVFTLTSGSNKDKKGGEAEKGKQVEKALDPQEYRKFQLKEKFI 64

Query: 72  LSHNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIK 131
           ++HN   FRF+LP    ILGLPIGQH+S R     G+EV +PYTP S D + GYFDL+IK
Sbjct: 65  VNHNTRIFRFALPNEDDILGLPIGQHISLRAVVG-GKEVYRPYTPISSDEERGYFDLLIK 124

Query: 132 MYPQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILE 191
           +Y +G MS +   M  GD + VKGPKG+F Y+ N  ++ GMLAGGTGITPM QV +AIL+
Sbjct: 125 VYEKGAMSGYVDNMFIGDSIEVKGPKGKFNYQPNMRKSIGMLAGGTGITPMLQVIKAILK 184

Query: 192 NPVDHTNAHLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEE 251
           NP D T   L++ N T EDILLK+ELD LA + P +F VYYVL+ PP+GW  GVGFV++E
Sbjct: 185 NPSDKTEISLVFGNITEEDILLKKELDELAEKHP-QFKVYYVLNNPPKGWTQGVGFVSKE 244

Query: 252 MIRLHCPGPSPNVRILRCGPPPMNKAMGAYLKTIGYTYQMQFQF 283
           +I    P PS    ++ CGPP MNKAM  +L+TIG+     F F
Sbjct: 245 IIESRLPSPSDQTMVIMCGPPMMNKAMTGHLETIGFNESNIFTF 286

BLAST of CmaCh20G000140 vs. ExPASy Swiss-Prot
Match: A1DHW1 (NADH-cytochrome b5 reductase 1 OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=cbr1 PE=3 SV=2)

HSP 1 Score: 264.2 bits (674), Expect = 1.7e-69
Identity = 133/272 (48.90%), Postives = 178/272 (65.44%), Query Frame = 0

Query: 10  RPEFLTVAVAAVAIGVATTYYFYITKKSKPQGCLDPDKFKEFKLVKRTQLSHNVAKFRFS 69
           + EF+  AVA  AI      +   T  SKP+  L+P +F+EF L ++T +SHNV  +RF+
Sbjct: 27  KKEFVPYAVALTAILAGLKLF---TGGSKPRKVLNPTEFQEFVLKEKTDISHNVCIYRFA 86

Query: 70  LPTPTSILGLPIGQHMSCRGK-DSEGEEVVKPYTPTSLDTDVGYFDLVIKMYPQGRMSHH 129
           LP P  ILGLPIGQH+S     + + +EVV+ YTP S D + GYFDL++K YPQG +S +
Sbjct: 87  LPRPADILGLPIGQHISLAATIEGQPKEVVRSYTPISSDNEAGYFDLLVKAYPQGNISKY 146

Query: 130 FREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENPV-----DH 189
              ++ GD + V+GPKG   Y  N     GM+AGGTGITPM Q+ +A++ N       D 
Sbjct: 147 LTTLKIGDTMKVRGPKGAMVYTPNMCRHIGMIAGGTGITPMLQIIKAVIRNRPRNGGNDT 206

Query: 190 TNAHLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMIRLH 249
           T   LI+AN   +DILLKEELD LA+  P  F++YYVL+ PP+GW GGVGFVT EMI+ H
Sbjct: 207 TKLDLIFANVNPDDILLKEELDKLAAEDPD-FNIYYVLNNPPQGWTGGVGFVTPEMIKEH 266

Query: 250 CPGPSPNVRILRCGPPPMNKAMGAYLKTIGYT 276
            P P+ +V+IL CGPPPM  AM    +++GYT
Sbjct: 267 LPAPASDVKILLCGPPPMISAMKKATESLGYT 294

BLAST of CmaCh20G000140 vs. ExPASy Swiss-Prot
Match: Q6BUX2 (NADH-cytochrome b5 reductase 1 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=CBR1 PE=3 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 3.7e-69
Identity = 128/263 (48.67%), Postives = 170/263 (64.64%), Query Frame = 0

Query: 13  FLTVAVAAVAIGVATTYYFY-ITKKSKPQGCLDPDKFKEFKLVKRTQLSHNVAKFRFSLP 72
           F+  A  A  I  A  YYF+ +++K+ P   L P+ F++F L+++T++SHN   +RF LP
Sbjct: 9   FIIFATVAAIISSAVAYYFFQLSRKNAP--VLKPNDFQKFPLIEKTRVSHNTCVYRFGLP 68

Query: 73  TPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMYPQGRMSHHFRE 132
             T  LGLPIGQH++  G     +EVV+ YTP S D ++GYFDL+IK Y  G +S H   
Sbjct: 69  RSTDRLGLPIGQHIAI-GATINDKEVVRSYTPISTDDELGYFDLLIKAYENGNISRHVES 128

Query: 133 MREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENPVDHTNAHLIYA 192
            + G+ + ++GPKG F Y    VE+FGM+AGGTGITPM+Q+  AIL NP D T   L+YA
Sbjct: 129 KKIGETIDIRGPKGFFTYTPGMVESFGMIAGGTGITPMYQILTAILRNPEDKTKVSLVYA 188

Query: 193 NATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMIRLHCPGPSPNV 252
           N T +DILLKEEL+ +A   P RF +YYVL+ PPE W GGVGFVT E++  H P  S   
Sbjct: 189 NVTEDDILLKEELNKMAREHPDRFKIYYVLNTPPENWTGGVGFVTPEIMDKHLPKASEAT 248

Query: 253 RILRCGPPPMNKAMGAYLKTIGY 275
            +L CGPPPM  AM      +GY
Sbjct: 249 NLLLCGPPPMISAMKKAAVGLGY 268

BLAST of CmaCh20G000140 vs. ExPASy Swiss-Prot
Match: Q4X0B5 (NADH-cytochrome b5 reductase 1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=cbr1 PE=3 SV=2)

HSP 1 Score: 262.3 bits (669), Expect = 6.3e-69
Identity = 132/272 (48.53%), Postives = 178/272 (65.44%), Query Frame = 0

Query: 10  RPEFLTVAVAAVAIGVATTYYFYITKKSKPQGCLDPDKFKEFKLVKRTQLSHNVAKFRFS 69
           + EF+  AVA  AI      +   T  +KP+  L+P +F+EF L ++  +SHNV  +RF+
Sbjct: 27  KKEFVPYAVAVTAILAGLKLF---TGGNKPRKVLNPTEFQEFVLKEKIDVSHNVCIYRFA 86

Query: 70  LPTPTSILGLPIGQHMSCRGK-DSEGEEVVKPYTPTSLDTDVGYFDLVIKMYPQGRMSHH 129
           LP PT ILGLPIGQH+S     + + +EVV+ YTP S D + GYFDL++K YPQG +S +
Sbjct: 87  LPRPTDILGLPIGQHISLAATIEGQPKEVVRSYTPISSDNEAGYFDLLVKAYPQGNISKY 146

Query: 130 FREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENPV-----DH 189
              ++ GD + V+GPKG   Y  N     GM+AGGTGITPM Q+ +A++ N       D 
Sbjct: 147 LTTLKIGDTMKVRGPKGAMVYTPNMCRHIGMIAGGTGITPMLQIIKAVIRNRPRNGGNDT 206

Query: 190 TNAHLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMIRLH 249
           T   LI+AN   +DILLKEELD LA+  P  F++YYVL+ PP+GW GGVGFVT EMI+ H
Sbjct: 207 TKLDLIFANVNPDDILLKEELDMLAAEDPD-FNIYYVLNNPPQGWTGGVGFVTPEMIKEH 266

Query: 250 CPGPSPNVRILRCGPPPMNKAMGAYLKTIGYT 276
            P P+ +V+IL CGPPPM  AM    +++GYT
Sbjct: 267 LPAPASDVKILLCGPPPMISAMKKATESLGYT 294

BLAST of CmaCh20G000140 vs. ExPASy TrEMBL
Match: A0A6J1J953 (NADH-cytochrome b5 reductase OS=Cucurbita maxima OX=3661 GN=LOC111483718 PE=3 SV=1)

HSP 1 Score: 587.4 bits (1513), Expect = 3.1e-164
Identity = 282/282 (100.00%), Postives = 282/282 (100.00%), Query Frame = 0

Query: 1   MATSFVHSHRPEFLTVAVAAVAIGVATTYYFYITKKSKPQGCLDPDKFKEFKLVKRTQLS 60
           MATSFVHSHRPEFLTVAVAAVAIGVATTYYFYITKKSKPQGCLDPDKFKEFKLVKRTQLS
Sbjct: 1   MATSFVHSHRPEFLTVAVAAVAIGVATTYYFYITKKSKPQGCLDPDKFKEFKLVKRTQLS 60

Query: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120
           HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY
Sbjct: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120

Query: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180
           PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP
Sbjct: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180

Query: 181 VDHTNAHLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240
           VDHTNAHLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI
Sbjct: 181 VDHTNAHLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240

Query: 241 RLHCPGPSPNVRILRCGPPPMNKAMGAYLKTIGYTYQMQFQF 283
           RLHCPGPSPNVRILRCGPPPMNKAMGAYLKTIGYTYQMQFQF
Sbjct: 241 RLHCPGPSPNVRILRCGPPPMNKAMGAYLKTIGYTYQMQFQF 282

BLAST of CmaCh20G000140 vs. ExPASy TrEMBL
Match: A0A6J1FXX1 (NADH-cytochrome b5 reductase OS=Cucurbita moschata OX=3662 GN=LOC111448782 PE=3 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 3.6e-160
Identity = 276/282 (97.87%), Postives = 276/282 (97.87%), Query Frame = 0

Query: 1   MATSFVHSHRPEFLTVAVAAVAIGVATTYYFYITKKSKPQGCLDPDKFKEFKLVKRTQLS 60
           MATSFVHSHRPEFLTVAVAAVAIGVAT YYFYITKKSKPQGCLDPD FKEFKLVKR QLS
Sbjct: 1   MATSFVHSHRPEFLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKRKQLS 60

Query: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120
           HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY
Sbjct: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120

Query: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180
           PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP
Sbjct: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180

Query: 181 VDHTNAHLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240
           VDHTN HLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI
Sbjct: 181 VDHTNVHLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240

Query: 241 RLHCPGPSPNVRILRCGPPPMNKAMGAYLKTIGYTYQMQFQF 283
           RLHCPGPSPNVRILRCGPPPMNKAM AYLKTIGYT QMQFQF
Sbjct: 241 RLHCPGPSPNVRILRCGPPPMNKAMAAYLKTIGYTSQMQFQF 282

BLAST of CmaCh20G000140 vs. ExPASy TrEMBL
Match: A0A6J1JE65 (NADH--cytochrome b5 reductase 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483718 PE=4 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 1.9e-145
Identity = 252/253 (99.60%), Postives = 253/253 (100.00%), Query Frame = 0

Query: 1   MATSFVHSHRPEFLTVAVAAVAIGVATTYYFYITKKSKPQGCLDPDKFKEFKLVKRTQLS 60
           MATSFVHSHRPEFLTVAVAAVAIGVATTYYFYITKKSKPQGCLDPDKFKEFKLVKRTQLS
Sbjct: 1   MATSFVHSHRPEFLTVAVAAVAIGVATTYYFYITKKSKPQGCLDPDKFKEFKLVKRTQLS 60

Query: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120
           HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY
Sbjct: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120

Query: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180
           PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP
Sbjct: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180

Query: 181 VDHTNAHLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240
           VDHTNAHLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI
Sbjct: 181 VDHTNAHLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240

Query: 241 RLHCPGPSPNVRI 254
           RLHCPGPSPNVR+
Sbjct: 241 RLHCPGPSPNVRV 253

BLAST of CmaCh20G000140 vs. ExPASy TrEMBL
Match: A0A5D3DDX7 (NADH-cytochrome b5 reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00930 PE=3 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 8.0e-144
Identity = 247/282 (87.59%), Postives = 260/282 (92.20%), Query Frame = 0

Query: 1   MATSFVHSHRPEFLTVAVAAVAIGVATTYYFYITKKSKPQGCLDPDKFKEFKLVKRTQLS 60
           M TSF    RPE LT+ VA VA+GVAT YYFY+ KKSK +GCLDPD FKEFKLVKRTQLS
Sbjct: 1   MPTSF----RPELLTITVAVVAVGVATAYYFYVAKKSKSKGCLDPDNFKEFKLVKRTQLS 60

Query: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120
           HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDS+GE+VVKPYTPT+LDTDVGYF+LVIKMY
Sbjct: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSQGEDVVKPYTPTTLDTDVGYFELVIKMY 120

Query: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180
           PQGRMSHHFREMREGD+LAVKGPKGRFKYE NEVEAFGMLAGGTGITPMFQVARAILENP
Sbjct: 121 PQGRMSHHFREMREGDYLAVKGPKGRFKYEPNEVEAFGMLAGGTGITPMFQVARAILENP 180

Query: 181 VDHTNAHLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240
           VD TN HLIYAN TYEDILLKEELDSLA+ FP+RFS+YYVL+QPPE WDGGVGFVTEEMI
Sbjct: 181 VDRTNVHLIYANVTYEDILLKEELDSLATTFPTRFSIYYVLNQPPEEWDGGVGFVTEEMI 240

Query: 241 RLHCPGPSPNVRILRCGPPPMNKAMGAYLKTIGYTYQMQFQF 283
           RLHCP PSPNVRILRCGPPPMNKAM AYL TIGYT QMQFQF
Sbjct: 241 RLHCPAPSPNVRILRCGPPPMNKAMAAYLNTIGYTSQMQFQF 278

BLAST of CmaCh20G000140 vs. ExPASy TrEMBL
Match: A0A1S3BN07 (NADH-cytochrome b5 reductase OS=Cucumis melo OX=3656 GN=LOC103491618 PE=3 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 8.0e-144
Identity = 247/282 (87.59%), Postives = 260/282 (92.20%), Query Frame = 0

Query: 1   MATSFVHSHRPEFLTVAVAAVAIGVATTYYFYITKKSKPQGCLDPDKFKEFKLVKRTQLS 60
           M TSF    RPE LT+ VA VA+GVAT YYFY+ KKSK +GCLDPD FKEFKLVKRTQLS
Sbjct: 1   MPTSF----RPELLTITVAVVAVGVATAYYFYVAKKSKSKGCLDPDNFKEFKLVKRTQLS 60

Query: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120
           HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDS+GE+VVKPYTPT+LDTDVGYF+LVIKMY
Sbjct: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSQGEDVVKPYTPTTLDTDVGYFELVIKMY 120

Query: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180
           PQGRMSHHFREMREGD+LAVKGPKGRFKYE NEVEAFGMLAGGTGITPMFQVARAILENP
Sbjct: 121 PQGRMSHHFREMREGDYLAVKGPKGRFKYEPNEVEAFGMLAGGTGITPMFQVARAILENP 180

Query: 181 VDHTNAHLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240
           VD TN HLIYAN TYEDILLKEELDSLA+ FP+RFS+YYVL+QPPE WDGGVGFVTEEMI
Sbjct: 181 VDRTNVHLIYANVTYEDILLKEELDSLATTFPTRFSIYYVLNQPPEEWDGGVGFVTEEMI 240

Query: 241 RLHCPGPSPNVRILRCGPPPMNKAMGAYLKTIGYTYQMQFQF 283
           RLHCP PSPNVRILRCGPPPMNKAM AYL TIGYT QMQFQF
Sbjct: 241 RLHCPAPSPNVRILRCGPPPMNKAMAAYLNTIGYTSQMQFQF 278

BLAST of CmaCh20G000140 vs. NCBI nr
Match: XP_022985761.1 (NADH--cytochrome b5 reductase 1-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 587.4 bits (1513), Expect = 6.5e-164
Identity = 282/282 (100.00%), Postives = 282/282 (100.00%), Query Frame = 0

Query: 1   MATSFVHSHRPEFLTVAVAAVAIGVATTYYFYITKKSKPQGCLDPDKFKEFKLVKRTQLS 60
           MATSFVHSHRPEFLTVAVAAVAIGVATTYYFYITKKSKPQGCLDPDKFKEFKLVKRTQLS
Sbjct: 1   MATSFVHSHRPEFLTVAVAAVAIGVATTYYFYITKKSKPQGCLDPDKFKEFKLVKRTQLS 60

Query: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120
           HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY
Sbjct: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120

Query: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180
           PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP
Sbjct: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180

Query: 181 VDHTNAHLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240
           VDHTNAHLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI
Sbjct: 181 VDHTNAHLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240

Query: 241 RLHCPGPSPNVRILRCGPPPMNKAMGAYLKTIGYTYQMQFQF 283
           RLHCPGPSPNVRILRCGPPPMNKAMGAYLKTIGYTYQMQFQF
Sbjct: 241 RLHCPGPSPNVRILRCGPPPMNKAMGAYLKTIGYTYQMQFQF 282

BLAST of CmaCh20G000140 vs. NCBI nr
Match: KAG6570317.1 (NADH--cytochrome b5 reductase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 574.7 bits (1480), Expect = 4.4e-160
Identity = 276/282 (97.87%), Postives = 276/282 (97.87%), Query Frame = 0

Query: 1   MATSFVHSHRPEFLTVAVAAVAIGVATTYYFYITKKSKPQGCLDPDKFKEFKLVKRTQLS 60
           MATSFVHSHRPEFLTVAVAAVAIGVAT YYFYITKKSKPQGCLDPD FKEFKLVKRTQLS
Sbjct: 1   MATSFVHSHRPEFLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKRTQLS 60

Query: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120
           HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY
Sbjct: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120

Query: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180
           PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP
Sbjct: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180

Query: 181 VDHTNAHLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240
           VDHTN HLIYANATYEDILLKEELDSL SRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI
Sbjct: 181 VDHTNVHLIYANATYEDILLKEELDSLVSRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240

Query: 241 RLHCPGPSPNVRILRCGPPPMNKAMGAYLKTIGYTYQMQFQF 283
           RLHCPGPSPNVRILRCGPPPMNKAM AYLKTIGYT QMQFQF
Sbjct: 241 RLHCPGPSPNVRILRCGPPPMNKAMAAYLKTIGYTSQMQFQF 282

BLAST of CmaCh20G000140 vs. NCBI nr
Match: XP_022944293.1 (NADH--cytochrome b5 reductase 1-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 573.9 bits (1478), Expect = 7.4e-160
Identity = 276/282 (97.87%), Postives = 276/282 (97.87%), Query Frame = 0

Query: 1   MATSFVHSHRPEFLTVAVAAVAIGVATTYYFYITKKSKPQGCLDPDKFKEFKLVKRTQLS 60
           MATSFVHSHRPEFLTVAVAAVAIGVAT YYFYITKKSKPQGCLDPD FKEFKLVKR QLS
Sbjct: 1   MATSFVHSHRPEFLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKRKQLS 60

Query: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120
           HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY
Sbjct: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120

Query: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180
           PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP
Sbjct: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180

Query: 181 VDHTNAHLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240
           VDHTN HLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI
Sbjct: 181 VDHTNVHLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240

Query: 241 RLHCPGPSPNVRILRCGPPPMNKAMGAYLKTIGYTYQMQFQF 283
           RLHCPGPSPNVRILRCGPPPMNKAM AYLKTIGYT QMQFQF
Sbjct: 241 RLHCPGPSPNVRILRCGPPPMNKAMAAYLKTIGYTSQMQFQF 282

BLAST of CmaCh20G000140 vs. NCBI nr
Match: XP_038901049.1 (NADH--cytochrome b5 reductase 1-like [Benincasa hispida])

HSP 1 Score: 534.3 bits (1375), Expect = 6.5e-148
Identity = 252/282 (89.36%), Postives = 265/282 (93.97%), Query Frame = 0

Query: 1   MATSFVHSHRPEFLTVAVAAVAIGVATTYYFYITKKSKPQGCLDPDKFKEFKLVKRTQLS 60
           M TSFV SHRPE LT AVA VAIGVAT YYFYITKKSKP+ CLDPD FKEFKL KRTQLS
Sbjct: 1   MPTSFVPSHRPELLTAAVAVVAIGVATAYYFYITKKSKPKACLDPDNFKEFKLAKRTQLS 60

Query: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120
           HNVAKFRFSLPTPTSILGLPIGQH+SCRGKDSEGE+VVKPYTPT+LDTDVGYFDLVIKMY
Sbjct: 61  HNVAKFRFSLPTPTSILGLPIGQHISCRGKDSEGEDVVKPYTPTTLDTDVGYFDLVIKMY 120

Query: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180
           PQGRMSHHFREMREGD+LAVKGPKGRFKYE NEVEAFGMLAGGTGITPMFQVARAILENP
Sbjct: 121 PQGRMSHHFREMREGDYLAVKGPKGRFKYEPNEVEAFGMLAGGTGITPMFQVARAILENP 180

Query: 181 VDHTNAHLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240
           VD TN HLIYAN TYEDILLKEELDS A+RFP+RF++YYVL+QPPEGWDGGVGFVTE+MI
Sbjct: 181 VDRTNVHLIYANVTYEDILLKEELDSFATRFPTRFTIYYVLNQPPEGWDGGVGFVTEQMI 240

Query: 241 RLHCPGPSPNVRILRCGPPPMNKAMGAYLKTIGYTYQMQFQF 283
           RLHCP PSPNVRILRCGPPPMNKAM AYL++IGYT QMQFQF
Sbjct: 241 RLHCPAPSPNVRILRCGPPPMNKAMAAYLQSIGYTSQMQFQF 282

BLAST of CmaCh20G000140 vs. NCBI nr
Match: XP_022985759.1 (NADH--cytochrome b5 reductase 1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 525.0 bits (1351), Expect = 4.0e-145
Identity = 252/253 (99.60%), Postives = 253/253 (100.00%), Query Frame = 0

Query: 1   MATSFVHSHRPEFLTVAVAAVAIGVATTYYFYITKKSKPQGCLDPDKFKEFKLVKRTQLS 60
           MATSFVHSHRPEFLTVAVAAVAIGVATTYYFYITKKSKPQGCLDPDKFKEFKLVKRTQLS
Sbjct: 1   MATSFVHSHRPEFLTVAVAAVAIGVATTYYFYITKKSKPQGCLDPDKFKEFKLVKRTQLS 60

Query: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120
           HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY
Sbjct: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120

Query: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180
           PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP
Sbjct: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180

Query: 181 VDHTNAHLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240
           VDHTNAHLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI
Sbjct: 181 VDHTNAHLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240

Query: 241 RLHCPGPSPNVRI 254
           RLHCPGPSPNVR+
Sbjct: 241 RLHCPGPSPNVRV 253

BLAST of CmaCh20G000140 vs. TAIR 10
Match: AT5G17770.1 (NADH:cytochrome B5 reductase 1 )

HSP 1 Score: 410.2 bits (1053), Expect = 1.3e-114
Identity = 193/283 (68.20%), Postives = 230/283 (81.27%), Query Frame = 0

Query: 1   MATSFVHS-HRPEFLTVAVAAVAIGVATTYYFYITKKSKPQGCLDPDKFKEFKLVKRTQL 60
           M T F+ +  R   L V VA VA+G    Y  ++T   K + CLDP+ FKEFKLVKR QL
Sbjct: 1   MDTEFLRTLDRQILLGVFVAFVAVGAGAAY--FLTSSKKRRVCLDPENFKEFKLVKRHQL 60

Query: 61  SHNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKM 120
           SHNVAKF F LPT TS+LGLPIGQH+SCRGKD +GE+V+KPYTPT+LD+DVG F+LVIKM
Sbjct: 61  SHNVAKFVFELPTSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPTTLDSDVGRFELVIKM 120

Query: 121 YPQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILEN 180
           YPQGRMSHHFREMR GD LAVKGPKGRFKY+  +  AFGMLAGG+GITPMFQVARAILEN
Sbjct: 121 YPQGRMSHHFREMRVGDHLAVKGPKGRFKYQPGQFRAFGMLAGGSGITPMFQVARAILEN 180

Query: 181 PVDHTNAHLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEM 240
           P D T  HLIYAN TY+DILLKEEL+ L + +P +F ++YVL+QPPE WDGGVGFV++EM
Sbjct: 181 PTDKTKVHLIYANVTYDDILLKEELEGLTTNYPEQFKIFYVLNQPPEVWDGGVGFVSKEM 240

Query: 241 IRLHCPGPSPNVRILRCGPPPMNKAMGAYLKTIGYTYQMQFQF 283
           I+ HCP P+ +++ILRCGPPPMNKAM A L+ +GY+ +MQFQF
Sbjct: 241 IQTHCPAPASDIQILRCGPPPMNKAMAANLEALGYSPEMQFQF 281

BLAST of CmaCh20G000140 vs. TAIR 10
Match: AT5G20080.1 (FAD/NAD(P)-binding oxidoreductase )

HSP 1 Score: 216.5 bits (550), Expect = 2.8e-56
Identity = 121/300 (40.33%), Postives = 179/300 (59.67%), Query Frame = 0

Query: 13  FLTVAVAAVAIGVATTYYFYIT------------KKSKPQGCLDPDKFKEFKLVKRTQLS 72
           F T A+AA++ G +   Y+Y+T            +++ P+  L+PDK+ EFKL    ++S
Sbjct: 34  FSTGAIAALSGGFS---YYYLTSGNNLVYLDQAKEETGPKTALNPDKWLEFKLQDTARVS 93

Query: 73  HNVAKFRFSLPTPTSILGLPIGQHMSCR---GKDSEGEE--VVKPYTPTSLDTDVGYFDL 132
           HN   FRFS   P++ LGL +   +  R   G ++EG+   V++PYTP S     GYFDL
Sbjct: 94  HNTQLFRFSF-DPSAELGLHVASCLLTRAPLGYNAEGKTKYVIRPYTPISDPEAKGYFDL 153

Query: 133 VIKMYPQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARA 192
           +IK+YP G+MS HF  ++ GD L VKGP  +FKY  N  +  GM+AGG+GITPM QV  A
Sbjct: 154 LIKVYPDGKMSQHFASLKPGDVLEVKGPVEKFKYSPNMKKHIGMIAGGSGITPMLQVIDA 213

Query: 193 ILENPVDHTNAHLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFV 252
           I++NP D+T   L+YAN + +DILLK++LD L +  P+   ++Y +D P + W GGVG++
Sbjct: 214 IVKNPEDNTQISLLYANVSPDDILLKQKLDVLQANHPN-LKIFYTVDNPTKNWKGGVGYI 273

Query: 253 TEEMIRLHCPGPSPNVRILRCGPPPMNKAMG-------------AYLKTIGYTYQMQFQF 283
           +++M     P P+ +  IL CGPP M + +                LK +GYT +M F+F
Sbjct: 274 SKDMALKGLPLPTDDTLILVCGPPGMMEHISGGKAPDWSQGEVKGILKELGYTEEMVFKF 328

BLAST of CmaCh20G000140 vs. TAIR 10
Match: AT1G37130.1 (nitrate reductase 2 )

HSP 1 Score: 180.6 bits (457), Expect = 1.7e-45
Identity = 94/230 (40.87%), Postives = 140/230 (60.87%), Query Frame = 0

Query: 52  KLVKRTQLSHNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVG 111
           +LV++T +SH+V KFRF+LP    +LGLP+G+H+      ++ +  ++ YTP+S    VG
Sbjct: 666 QLVEKTSISHDVRKFRFALPVEDMVLGLPVGKHIFLCATIND-KLCLRAYTPSSTVDVVG 725

Query: 112 YFDLVIKMY-----PQ----GRMSHHFREMREGDFLAVKGPKGRFKY----------EAN 171
           YF+LV+K+Y     P+    G MS +   +  G  L +KGP G  +Y          +  
Sbjct: 726 YFELVVKIYFGGVHPRFPNGGLMSQYLDSLPIGSTLEIKGPLGHVEYLGKGSFTVHGKPK 785

Query: 172 EVEAFGMLAGGTGITPMFQVARAILENPVDHTNAHLIYANATYEDILLKEELDSLASRFP 231
             +   MLAGGTGITP++Q+ +AIL++P D T  ++IYAN T EDILL+EELD  A ++P
Sbjct: 786 FADKLAMLAGGTGITPVYQIIQAILKDPEDETEMYVIYANRTEEDILLREELDGWAEQYP 845

Query: 232 SRFSVYYVLDQPPEGWDGGVGFVTEEMIRLHCP-GPSPNVRILRCGPPPM 262
            R  V+YV++   EGW    GF++E ++R H P G   +   + CGPPPM
Sbjct: 846 DRLKVWYVVESAKEGWAYSTGFISEAIMREHIPDGLDGSALAMACGPPPM 894

BLAST of CmaCh20G000140 vs. TAIR 10
Match: AT1G77760.1 (nitrate reductase 1 )

HSP 1 Score: 176.8 bits (447), Expect = 2.5e-44
Identity = 93/244 (38.11%), Postives = 144/244 (59.02%), Query Frame = 0

Query: 52  KLVKRTQLSHNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVG 111
           +L+++T +SH+V KFRF+LP+    LGLP+G+H+      ++ +  ++ YTPTS    VG
Sbjct: 666 RLIEKTSISHDVRKFRFALPSEDQQLGLPVGKHVFVCANIND-KLCLRAYTPTSAIDAVG 725

Query: 112 YFDLVIKMY-----PQ----GRMSHHFREMREGDFLAVKGPKGRFKYEANE--------- 171
           + DLV+K+Y     P+    G MS H   +  G  + +KGP G  +Y+            
Sbjct: 726 HIDLVVKVYFKDVHPRFPNGGLMSQHLDSLPIGSMIDIKGPLGHIEYKGKGNFLVSGKPK 785

Query: 172 -VEAFGMLAGGTGITPMFQVARAILENPVDHTNAHLIYANATYEDILLKEELDSLASRFP 231
             +   MLAGGTGITP++Q+ ++IL +P D T  +++YAN T +DIL++EEL+  AS+  
Sbjct: 786 FAKKLAMLAGGTGITPIYQIIQSILSDPEDETEMYVVYANRTEDDILVREELEGWASKHK 845

Query: 232 SRFSVYYVLDQPPEGWDGGVGFVTEEMIRLHCP-GPSPNVRILRCGPPPMNK-AMGAYLK 275
            R  ++YV++   EGW    GF+TE ++R H P G       L CGPPPM + A+   L+
Sbjct: 846 ERLKIWYVVEIAKEGWSYSTGFITEAVLREHIPEGLEGESLALACGPPPMIQFALQPNLE 905

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZNT11.9e-11368.20NADH--cytochrome b5 reductase 1 OS=Arabidopsis thaliana OX=3702 GN=CBR1 PE=1 SV=... [more]
Q54NC17.4e-7047.18NADH-cytochrome b5 reductase 1 OS=Dictyostelium discoideum OX=44689 GN=cyb5r1 PE... [more]
A1DHW11.7e-6948.90NADH-cytochrome b5 reductase 1 OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3... [more]
Q6BUX23.7e-6948.67NADH-cytochrome b5 reductase 1 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS... [more]
Q4X0B56.3e-6948.53NADH-cytochrome b5 reductase 1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / A... [more]
Match NameE-valueIdentityDescription
A0A6J1J9533.1e-164100.00NADH-cytochrome b5 reductase OS=Cucurbita maxima OX=3661 GN=LOC111483718 PE=3 SV... [more]
A0A6J1FXX13.6e-16097.87NADH-cytochrome b5 reductase OS=Cucurbita moschata OX=3662 GN=LOC111448782 PE=3 ... [more]
A0A6J1JE651.9e-14599.60NADH--cytochrome b5 reductase 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A5D3DDX78.0e-14487.59NADH-cytochrome b5 reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3BN078.0e-14487.59NADH-cytochrome b5 reductase OS=Cucumis melo OX=3656 GN=LOC103491618 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022985761.16.5e-164100.00NADH--cytochrome b5 reductase 1-like isoform X3 [Cucurbita maxima][more]
KAG6570317.14.4e-16097.87NADH--cytochrome b5 reductase 1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022944293.17.4e-16097.87NADH--cytochrome b5 reductase 1-like isoform X3 [Cucurbita moschata][more]
XP_038901049.16.5e-14889.36NADH--cytochrome b5 reductase 1-like [Benincasa hispida][more]
XP_022985759.14.0e-14599.60NADH--cytochrome b5 reductase 1-like isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G17770.11.3e-11468.20NADH:cytochrome B5 reductase 1 [more]
AT5G20080.12.8e-5640.33FAD/NAD(P)-binding oxidoreductase [more]
AT1G37130.11.7e-4540.87nitrate reductase 2 [more]
AT1G77760.12.5e-4438.11nitrate reductase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001709Flavoprotein pyridine nucleotide cytochrome reductasePRINTSPR00371FPNCRcoord: 196..207
score: 44.16
coord: 252..260
score: 37.29
coord: 158..177
score: 41.26
IPR001834NADH:cytochrome b5 reductase-likePRINTSPR00406CYTB5RDTASEcoord: 135..149
score: 49.44
coord: 196..207
score: 64.76
coord: 99..106
score: 57.29
coord: 77..88
score: 53.47
coord: 158..177
score: 66.67
coord: 252..260
score: 59.26
IPR008333Flavoprotein pyridine nucleotide cytochrome reductase-like, FAD-binding domainPFAMPF00970FAD_binding_6coord: 51..149
e-value: 7.6E-32
score: 109.6
IPR039261Ferredoxin-NADP reductase (FNR), nucleotide-binding domainGENE3D3.40.50.80coord: 142..279
e-value: 5.3E-41
score: 141.7
IPR039261Ferredoxin-NADP reductase (FNR), nucleotide-binding domainSUPERFAMILY52343Ferredoxin reductase-like, C-terminal NADP-linked domaincoord: 138..275
IPR001433Oxidoreductase FAD/NAD(P)-bindingPFAMPF00175NAD_binding_1coord: 159..264
e-value: 5.2E-29
score: 101.1
NoneNo IPR availableGENE3D2.40.30.10Translation factorscoord: 41..141
e-value: 6.9E-35
score: 121.7
NoneNo IPR availablePANTHERPTHR19370:SF204NADH-CYTOCHROME B5 REDUCTASEcoord: 6..282
NoneNo IPR availablePANTHERPTHR19370NADH-CYTOCHROME B5 REDUCTASEcoord: 6..282
NoneNo IPR availableCDDcd06183cyt_b5_reduct_likecoord: 51..282
e-value: 7.80798E-113
score: 322.979
IPR017927FAD-binding domain, ferredoxin reductase-typePROSITEPS51384FAD_FRcoord: 46..150
score: 14.769834
IPR017938Riboflavin synthase-like beta-barrelSUPERFAMILY63380Riboflavin synthase domain-likecoord: 42..150

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh20G000140.1CmaCh20G000140.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0022900 electron transport chain
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004128 cytochrome-b5 reductase activity, acting on NAD(P)H
molecular_function GO:0016491 oxidoreductase activity