CmaCh19G006810 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh19G006810
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Cysteine protease
Location	Cma_Chr19: 7076395 .. 7077268 (+)
RNA-Seq Expression	CmaCh19G006810
Synteny	CmaCh19G006810

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCGGAGTTTCTTTTTGTTCGCCGCCACCGCCGCCACCCTCTGCTCATCGGAGTCTTTGGCCTCATCGCATTCCATCGAATGCGACGGCGATACGCTGATTCGTCAAGTTATCGACGATGGAGATTTCAAGCGTCACTCTCTCGGAGCGGAACATCATTTTTCGCTCTTCAAGCGAAGGTTCGGGAAATCATATGCCACCGAGGAAGAGCACGATCTTAGGCTTAAGATCTTCAAGGCTAATATGCGACAGGCTCTGCGCCATCAGTCATTTGATCCATCCGTCATTCATGGCGTCACTCAGTTCTCCGATTTGATTCCTTCCGAGTTTAGGGAGGCCTTTCTAGGGCTCCAAAGAAATCGTCTCAGGCTTCCTGTTGATACTAATGCGGCTCCGATTCTTCCTACGGAGAACCTTCCGATTCATTTTGATTGGAGAGAACGTAGTGTTTTAACTGCTGTAAAAAATCAGGTACCGTACTATATCTCAAATCTCCCGTTTAATTGCGATCTTTGAAGTTCTAGGCGTGTTTTGATTCTTGTTCTTATTCTTCCTTTCTGTAGGATACACCTATTTCATTTGGTTACCTATGATTTCTGTTTTTGAACGAATTTTAATCTGTGGAAGTTTGAAGGACAATTGTAAATCTGAAATGCACTTATACTTGTGTCTTCATCCCTAGTTCTTACAGCATATATAGATATTTTACAAGAAGCTGATTGTATTCTAAAGCAATTTCTTTATAATTATCTGCTATCTTACCATTTGGGGTTGTTAAAAAGTCTAAAATCAGCTTTGAACATCTACATTCGATCAGGGATCTTGCGGATCCTGCTGGAGTTTCAGTACAACCGGTGCCCTAGAAGGTTAA

mRNA sequence

ATGGATCGGAGTTTCTTTTTGTTCGCCGCCACCGCCGCCACCCTCTGCTCATCGGAGTCTTTGGCCTCATCGCATTCCATCGAATGCGACGGCGATACGCTGATTCGTCAAGTTATCGACGATGGAGATTTCAAGCGTCACTCTCTCGGAGCGGAACATCATTTTTCGCTCTTCAAGCGAAGGTTCGGGAAATCATATGCCACCGAGGAAGAGCACGATCTTAGGCTTAAGATCTTCAAGGCTAATATGCGACAGGCTCTGCGCCATCAGTCATTTGATCCATCCGTCATTCATGGCGTCACTCAGTTCTCCGATTTGATTCCTTCCGAGTTTAGGGAGGCCTTTCTAGGGCTCCAAAGAAATCGTCTCAGGCTTCCTGTTGATACTAATGCGGCTCCGATTCTTCCTACGGAGAACCTTCCGATTCATTTTGATTGGAGAGAACGTAGTGTTTTAACTGCTGTAAAAAATCAGGGATCTTGCGGATCCTGCTGGAGTTTCAGTACAACCGGTGCCCTAGAAGGTTAA

Coding sequence (CDS)

ATGGATCGGAGTTTCTTTTTGTTCGCCGCCACCGCCGCCACCCTCTGCTCATCGGAGTCTTTGGCCTCATCGCATTCCATCGAATGCGACGGCGATACGCTGATTCGTCAAGTTATCGACGATGGAGATTTCAAGCGTCACTCTCTCGGAGCGGAACATCATTTTTCGCTCTTCAAGCGAAGGTTCGGGAAATCATATGCCACCGAGGAAGAGCACGATCTTAGGCTTAAGATCTTCAAGGCTAATATGCGACAGGCTCTGCGCCATCAGTCATTTGATCCATCCGTCATTCATGGCGTCACTCAGTTCTCCGATTTGATTCCTTCCGAGTTTAGGGAGGCCTTTCTAGGGCTCCAAAGAAATCGTCTCAGGCTTCCTGTTGATACTAATGCGGCTCCGATTCTTCCTACGGAGAACCTTCCGATTCATTTTGATTGGAGAGAACGTAGTGTTTTAACTGCTGTAAAAAATCAGGGATCTTGCGGATCCTGCTGGAGTTTCAGTACAACCGGTGCCCTAGAAGGTTAA

Protein sequence

MDRSFFLFAATAATLCSSESLASSHSIECDGDTLIRQVIDDGDFKRHSLGAEHHFSLFKRRFGKSYATEEEHDLRLKIFKANMRQALRHQSFDPSVIHGVTQFSDLIPSEFREAFLGLQRNRLRLPVDTNAAPILPTENLPIHFDWRERSVLTAVKNQGSCGSCWSFSTTGALEG

Homology

[Close]

BLAST of CmaCh19G006810 vs. ExPASy Swiss-Prot
Match: Q9SUL1 (Probable cysteine protease RD19C OS=Arabidopsis thaliana OX=3702 GN=RD19C PE=2 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 5.5e-47
Identity = 97/176 (55.11%), Postives = 116/176 (65.91%), Query Frame = 0


Query: 1   MDRSFFLFAATAATLCSS-ESLASSHSIECDGDTLIRQVIDDGDFKRHSLGAEHHFSLFK 60
           MDR  F F   A  L  S  S   S  +       IRQV+ + +     L AEHHF+LFK
Sbjct: 1   MDRVVFFFLIAATLLAGSLGSTVISGEVTDGFVNPIRQVVPE-ENDEQLLNAEHHFTLFK 60

Query: 61  RRFGKSYATEEEHDLRLKIFKANMRQALRHQSFDPSVIHGVTQFSDLIPSEFREAFLGLQ 120
            ++ K+YAT+ EHD R ++FKAN+R+A R+Q  DPS +HGVTQFSDL P EFR  FLGL+
Sbjct: 61  SKYEKTYATQVEHDHRFRVFKANLRRARRNQLLDPSAVHGVTQFSDLTPKEFRRKFLGLK 120

Query: 121 RNRLRLPVDTNAAPILPTENLPIHFDWRERSVLTAVKNQGSCGSCWSFSTTGALEG 176
           R   RLP DT  APILPT +LP  FDWRE+  +T VKNQG CGSCWSFS  GALEG
Sbjct: 121 RRGFRLPTDTQTAPILPTSDLPTEFDWREQGAVTPVKNQGMCGSCWSFSAIGALEG 175

[Close]

BLAST of CmaCh19G006810 vs. ExPASy Swiss-Prot
Match: P43296 (Cysteine protease RD19A OS=Arabidopsis thaliana OX=3702 GN=RD19A PE=1 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 9.3e-47
Identity = 94/153 (61.44%), Postives = 110/153 (71.90%), Query Frame = 0


Query: 23  SSHSIECDGDTLIRQVIDDGDFKRHSLGAEHHFSLFKRRFGKSYATEEEHDLRLKIFKAN 82
           SS  +    D +IRQV+  G  +   L +E HFSLFKR+FGK YA+ EEHD R  +FKAN
Sbjct: 21  SSSDVNDGDDLVIRQVV--GGAEPQVLTSEDHFSLFKRKFGKVYASNEEHDYRFSVFKAN 80

Query: 83  MRQALRHQSFDPSVIHGVTQFSDLIPSEFREAFLGLQRNRLRLPVDTNAAPILPTENLPI 142
           +R+A RHQ  DPS  HGVTQFSDL  SEFR+  LG+ R+  +LP D N APILPTENLP 
Sbjct: 81  LRRARRHQKLDPSATHGVTQFSDLTRSEFRKKHLGV-RSGFKLPKDANKAPILPTENLPE 140

Query: 143 HFDWRERSVLTAVKNQGSCGSCWSFSTTGALEG 176
            FDWR+   +T VKNQGSCGSCWSFS TGALEG
Sbjct: 141 DFDWRDHGAVTPVKNQGSCGSCWSFSATGALEG 170

[Close]

BLAST of CmaCh19G006810 vs. ExPASy Swiss-Prot
Match: P25804 (Cysteine proteinase 15A OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 4.6e-46
Identity = 98/179 (54.75%), Postives = 122/179 (68.16%), Query Frame = 0


Query: 1   MDRSF----FLFAATAATLCSSESLASSHSIECDGDTLIRQVIDDGDFKRHSLGAEHHFS 60
           MDR F    FLFAA A  +    +         + D +IRQV+D+ +   H L AEHHF+
Sbjct: 1   MDRRFLFALFLFAAVATAVTDDTN---------NDDFIIRQVVDNEE--DHLLNAEHHFT 60

Query: 61  LFKRRFGKSYATEEEHDLRLKIFKANMRQALRHQSFDPSVIHGVTQFSDLIPSEFREAFL 120
            FK +F KSYAT+EEHD R  +FK+N+ +A  HQ+ DP+  HG+T+FSDL  SEFR  FL
Sbjct: 61  SFKSKFSKSYATKEEHDYRFGVFKSNLIKAKLHQNRDPTAEHGITKFSDLTASEFRRQFL 120

Query: 121 GLQRNRLRLPVDTNAAPILPTENLPIHFDWRERSVLTAVKNQGSCGSCWSFSTTGALEG 176
           GL++ RLRLP     APILPT NLP  FDWRE+  +T VK+QGSCGSCW+FSTTGALEG
Sbjct: 121 GLKK-RLRLPAHAQKAPILPTTNLPEDFDWREKGAVTPVKDQGSCGSCWAFSTTGALEG 167

[Close]

BLAST of CmaCh19G006810 vs. ExPASy Swiss-Prot
Match: P43295 (Probable cysteine protease RD19B OS=Arabidopsis thaliana OX=3702 GN=RD19B PE=2 SV=2)

HSP 1 Score: 184.1 bits (466), Expect = 1.3e-45
Identity = 92/152 (60.53%), Postives = 111/152 (73.03%), Query Frame = 0


Query: 24  SHSIECDGDTLIRQVIDDGDFKRHSLGAEHHFSLFKRRFGKSYATEEEHDLRLKIFKANM 83
           S S+  D D LIRQV+D+ + K   L +E HF+LFK++FGK Y + EEH  R  +FKAN+
Sbjct: 19  SVSVCGDEDVLIRQVVDETEPK--VLSSEDHFTLFKKKFGKVYGSIEEHYYRFSVFKANL 78

Query: 84  RQALRHQSFDPSVIHGVTQFSDLIPSEFREAFLGLQRNRLRLPVDTNAAPILPTENLPIH 143
            +A+RHQ  DPS  HGVTQFSDL  SEFR   LG+ +   +LP D N APILPT+NLP  
Sbjct: 79  LRAMRHQKMDPSARHGVTQFSDLTRSEFRRKHLGV-KGGFKLPKDANQAPILPTQNLPEE 138

Query: 144 FDWRERSVLTAVKNQGSCGSCWSFSTTGALEG 176
           FDWR+R  +T VKNQGSCGSCWSFSTTGALEG
Sbjct: 139 FDWRDRGAVTPVKNQGSCGSCWSFSTTGALEG 167

[Close]

BLAST of CmaCh19G006810 vs. ExPASy Swiss-Prot
Match: Q10716 (Cysteine proteinase 1 OS=Zea mays OX=4577 GN=CCP1 PE=2 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 1.0e-40
Identity = 86/166 (51.81%), Postives = 110/166 (66.27%), Query Frame = 0


Query: 14  TLCSSESLASSHSIECDGDTLIRQVIDDGDFKRHSLGAEHHFSLFKRRFGKSYATEEEHD 73
           +L S+ ++A++   E   D LIRQV+  GD     L AE HF  F +RFGKSY   +EH 
Sbjct: 10  SLASAAAVAAAVDAE---DPLIRQVVPGGDDNDLELNAESHFLSFVQRFGKSYKDADEHA 69

Query: 74  LRLKIFKANMRQALRHQSFDPSVIHGVTQFSDLIPSEFREAFLGLQRNR----LRLPVDT 133
            RL +FK N+R+A RHQ  DPS  HGVT+FSDL P+EFR  +LGL+++R      L    
Sbjct: 70  YRLSVFKDNLRRARRHQLLDPSAEHGVTKFSDLTPAEFRRTYLGLRKSRRALLRELGESA 129

Query: 134 NAAPILPTENLPIHFDWRERSVLTAVKNQGSCGSCWSFSTTGALEG 176
           + AP+LPT+ LP  FDWR+   +  VKNQGSCGSCWSFS +GALEG
Sbjct: 130 HEAPVLPTDGLPDDFDWRDHGAVGPVKNQGSCGSCWSFSASGALEG 172

[Close]

BLAST of CmaCh19G006810 vs. ExPASy TrEMBL
Match: A0A0A0K2B5 (Papain-like cysteine proteinase isoform I OS=Cucumis sativus OX=3659 GN=Csa_7G004120 PE=3 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 7.1e-74
Identity = 143/179 (79.89%), Postives = 154/179 (86.03%), Query Frame = 0


Query: 1   MDRSFFLFA---ATAATLCSSESLASSHSIECDGDTLIRQVID-DGDFKRHSLGAEHHFS 60
           MDR+FFLFA   A  ATLCSSE L S HS+E DGD LIRQV++ DGDF  H+LGAEHHFS
Sbjct: 1   MDRNFFLFAVITAVTATLCSSEPLVSQHSVEHDGDPLIRQVVENDGDFNHHALGAEHHFS 60

Query: 61  LFKRRFGKSYATEEEHDLRLKIFKANMRQALRHQSFDPSVIHGVTQFSDLIPSEFREAFL 120
           LFKRRFGKSYATEEEHD R KIFKANMR+A RHQSFDPS IHGVTQFSDL P EFR+AFL
Sbjct: 61  LFKRRFGKSYATEEEHDRRFKIFKANMRRAERHQSFDPSAIHGVTQFSDLTPFEFRKAFL 120

Query: 121 GLQRNRLRLPVDTNAAPILPTENLPIHFDWRERSVLTAVKNQGSCGSCWSFSTTGALEG 176
           GL+ +RLRLPVDTNAAPILPTENLPI FDWR+   +T VKNQGSCGSCWSFSTTGALEG
Sbjct: 121 GLRGHRLRLPVDTNAAPILPTENLPIDFDWRQHGGVTRVKNQGSCGSCWSFSTTGALEG 179

[Close]

BLAST of CmaCh19G006810 vs. ExPASy TrEMBL
Match: A0A1S3BIU9 (cysteine proteinase RD19a-like OS=Cucumis melo OX=3656 GN=LOC103490359 PE=3 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 7.1e-74
Identity = 142/177 (80.23%), Postives = 154/177 (87.01%), Query Frame = 0


Query: 1   MDRSFFLFAA-TAATLCSSESLASSHSIECDGDTLIRQVI-DDGDFKRHSLGAEHHFSLF 60
           MD +FFLFA  TAATLC SESL S HS++ D D  IRQV+ DDGDF RH+LGAEHHFSLF
Sbjct: 1   MDLNFFLFAVITAATLCLSESLTSPHSVQHDRDPFIRQVVEDDGDFNRHALGAEHHFSLF 60

Query: 61  KRRFGKSYATEEEHDLRLKIFKANMRQALRHQSFDPSVIHGVTQFSDLIPSEFREAFLGL 120
           KRRFGKSYATEEEHD R KIFKANMR+A RHQSFDPS IHG+TQFSDL P EFR+AFLGL
Sbjct: 61  KRRFGKSYATEEEHDRRFKIFKANMRRAERHQSFDPSAIHGITQFSDLTPFEFRKAFLGL 120

Query: 121 QRNRLRLPVDTNAAPILPTENLPIHFDWRERSVLTAVKNQGSCGSCWSFSTTGALEG 176
           + +RLRLPVDTNAAPILPTENLPI FDWRER  +T VKNQGSCGSCWSFSTTGA+EG
Sbjct: 121 RGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVTRVKNQGSCGSCWSFSTTGAIEG 177

[Close]

BLAST of CmaCh19G006810 vs. ExPASy TrEMBL
Match: A0A5A7SS65 (Cysteine proteinase RD19a-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold142G00480 PE=3 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 3.5e-73
Identity = 141/177 (79.66%), Postives = 153/177 (86.44%), Query Frame = 0


Query: 1   MDRSFFLFAA-TAATLCSSESLASSHSIECDGDTLIRQVI-DDGDFKRHSLGAEHHFSLF 60
           MD +FFLFA  TAATLC SESL S HS++ D D  IRQV+ DD DF RH+LGAEHHFSLF
Sbjct: 1   MDLNFFLFAVITAATLCLSESLTSPHSVQHDRDPFIRQVVEDDSDFNRHALGAEHHFSLF 60

Query: 61  KRRFGKSYATEEEHDLRLKIFKANMRQALRHQSFDPSVIHGVTQFSDLIPSEFREAFLGL 120
           KRRFGKSYATEEEHD R KIFKANMR+A RHQSFDPS IHG+TQFSDL P EFR+AFLGL
Sbjct: 61  KRRFGKSYATEEEHDRRFKIFKANMRRAERHQSFDPSAIHGITQFSDLTPFEFRKAFLGL 120

Query: 121 QRNRLRLPVDTNAAPILPTENLPIHFDWRERSVLTAVKNQGSCGSCWSFSTTGALEG 176
           + +RLRLPVDTNAAPILPTENLPI FDWRER  +T VKNQGSCGSCWSFSTTGA+EG
Sbjct: 121 RGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVTRVKNQGSCGSCWSFSTTGAIEG 177

[Close]

BLAST of CmaCh19G006810 vs. ExPASy TrEMBL
Match: A0A6J1F4R7 (probable cysteine protease RD19B OS=Cucurbita moschata OX=3662 GN=LOC111440857 PE=3 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 5.1e-64
Identity = 129/178 (72.47%), Postives = 146/178 (82.02%), Query Frame = 0


Query: 1   MDRSFFLFA---ATAATLCSSESLASSHSIECDGDTLIRQVIDDGDFKRHSLGAEHHFSL 60
           MDRSFFLF+   A AA LCSSESLAS++S+      LIRQVIDDG+     L AEHHF L
Sbjct: 1   MDRSFFLFSVVIAAAAALCSSESLASTYSV----SPLIRQVIDDGESSNLPLQAEHHFLL 60

Query: 61  FKRRFGKSYATEEEHDLRLKIFKANMRQALRHQSFDPSVIHGVTQFSDLIPSEFREAFLG 120
           FKR+FGKSYATEEEH+ R +IFKANMR+ALRHQSFDPS IHGVTQFSDL  SEF++ FLG
Sbjct: 61  FKRKFGKSYATEEEHNRRFRIFKANMRRALRHQSFDPSAIHGVTQFSDLTVSEFQKTFLG 120

Query: 121 LQRNRLRLPVDTNAAPILPTENLPIHFDWRERSVLTAVKNQGSCGSCWSFSTTGALEG 176
           L+ +RL+LP+D N APILPTENLP  FDWRER  +T VKNQG+CGSCWSFSTTGALEG
Sbjct: 121 LRGHRLKLPLDANQAPILPTENLPGGFDWRERGAVTPVKNQGTCGSCWSFSTTGALEG 174

[Close]

BLAST of CmaCh19G006810 vs. ExPASy TrEMBL
Match: A0A5D3DFH2 (Cysteine protease RD19A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1428G00620 PE=3 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 2.1e-62
Identity = 125/160 (78.12%), Postives = 136/160 (85.00%), Query Frame = 0


Query: 1   MDRSFFLFAA-TAATLCSSESLASSHSIECDGDTLIRQVI-DDGDFKRHSLGAEHHFSLF 60
           MD +FFLFA  TAATLC SESL S HS++ D D  IRQV+ DD DF RH+LGAEHHFSLF
Sbjct: 1   MDLNFFLFAVITAATLCLSESLTSPHSVQHDRDPFIRQVVEDDSDFNRHALGAEHHFSLF 60

Query: 61  KRRFGKSYATEEEHDLRLKIFKANMRQALRHQSFDPSVIHGVTQFSDLIPSEFREAFLGL 120
           KRRFGKSYATEEEHD R KIFKANMR+A RHQSFDPS IHG+TQFSDL P EFR+AFLGL
Sbjct: 61  KRRFGKSYATEEEHDRRFKIFKANMRRAERHQSFDPSAIHGITQFSDLTPFEFRKAFLGL 120

Query: 121 QRNRLRLPVDTNAAPILPTENLPIHFDWRERSVLTAVKNQ 159
           + +RLRLPVDTNAAPILPTENLPI FDWRER  +T VKNQ
Sbjct: 121 RGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVTRVKNQ 160

[Close]

BLAST of CmaCh19G006810 vs. NCBI nr
Match: XP_004148554.2 (cysteine protease RD19A [Cucumis sativus] >KGN43159.1 hypothetical protein Csa_020172 [Cucumis sativus])

HSP 1 Score: 286.6 bits (732), Expect = 1.5e-73
Identity = 143/179 (79.89%), Postives = 154/179 (86.03%), Query Frame = 0


Query: 1   MDRSFFLFA---ATAATLCSSESLASSHSIECDGDTLIRQVID-DGDFKRHSLGAEHHFS 60
           MDR+FFLFA   A  ATLCSSE L S HS+E DGD LIRQV++ DGDF  H+LGAEHHFS
Sbjct: 1   MDRNFFLFAVITAVTATLCSSEPLVSQHSVEHDGDPLIRQVVENDGDFNHHALGAEHHFS 60

Query: 61  LFKRRFGKSYATEEEHDLRLKIFKANMRQALRHQSFDPSVIHGVTQFSDLIPSEFREAFL 120
           LFKRRFGKSYATEEEHD R KIFKANMR+A RHQSFDPS IHGVTQFSDL P EFR+AFL
Sbjct: 61  LFKRRFGKSYATEEEHDRRFKIFKANMRRAERHQSFDPSAIHGVTQFSDLTPFEFRKAFL 120

Query: 121 GLQRNRLRLPVDTNAAPILPTENLPIHFDWRERSVLTAVKNQGSCGSCWSFSTTGALEG 176
           GL+ +RLRLPVDTNAAPILPTENLPI FDWR+   +T VKNQGSCGSCWSFSTTGALEG
Sbjct: 121 GLRGHRLRLPVDTNAAPILPTENLPIDFDWRQHGGVTRVKNQGSCGSCWSFSTTGALEG 179

[Close]

BLAST of CmaCh19G006810 vs. NCBI nr
Match: XP_008448073.1 (PREDICTED: cysteine proteinase RD19a-like [Cucumis melo])

HSP 1 Score: 286.6 bits (732), Expect = 1.5e-73
Identity = 142/177 (80.23%), Postives = 154/177 (87.01%), Query Frame = 0


Query: 1   MDRSFFLFAA-TAATLCSSESLASSHSIECDGDTLIRQVI-DDGDFKRHSLGAEHHFSLF 60
           MD +FFLFA  TAATLC SESL S HS++ D D  IRQV+ DDGDF RH+LGAEHHFSLF
Sbjct: 1   MDLNFFLFAVITAATLCLSESLTSPHSVQHDRDPFIRQVVEDDGDFNRHALGAEHHFSLF 60

Query: 61  KRRFGKSYATEEEHDLRLKIFKANMRQALRHQSFDPSVIHGVTQFSDLIPSEFREAFLGL 120
           KRRFGKSYATEEEHD R KIFKANMR+A RHQSFDPS IHG+TQFSDL P EFR+AFLGL
Sbjct: 61  KRRFGKSYATEEEHDRRFKIFKANMRRAERHQSFDPSAIHGITQFSDLTPFEFRKAFLGL 120

Query: 121 QRNRLRLPVDTNAAPILPTENLPIHFDWRERSVLTAVKNQGSCGSCWSFSTTGALEG 176
           + +RLRLPVDTNAAPILPTENLPI FDWRER  +T VKNQGSCGSCWSFSTTGA+EG
Sbjct: 121 RGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVTRVKNQGSCGSCWSFSTTGAIEG 177

[Close]

BLAST of CmaCh19G006810 vs. NCBI nr
Match: KAA0033790.1 (cysteine proteinase RD19a-like [Cucumis melo var. makuwa])

HSP 1 Score: 284.3 bits (726), Expect = 7.3e-73
Identity = 141/177 (79.66%), Postives = 153/177 (86.44%), Query Frame = 0


Query: 1   MDRSFFLFAA-TAATLCSSESLASSHSIECDGDTLIRQVI-DDGDFKRHSLGAEHHFSLF 60
           MD +FFLFA  TAATLC SESL S HS++ D D  IRQV+ DD DF RH+LGAEHHFSLF
Sbjct: 1   MDLNFFLFAVITAATLCLSESLTSPHSVQHDRDPFIRQVVEDDSDFNRHALGAEHHFSLF 60

Query: 61  KRRFGKSYATEEEHDLRLKIFKANMRQALRHQSFDPSVIHGVTQFSDLIPSEFREAFLGL 120
           KRRFGKSYATEEEHD R KIFKANMR+A RHQSFDPS IHG+TQFSDL P EFR+AFLGL
Sbjct: 61  KRRFGKSYATEEEHDRRFKIFKANMRRAERHQSFDPSAIHGITQFSDLTPFEFRKAFLGL 120

Query: 121 QRNRLRLPVDTNAAPILPTENLPIHFDWRERSVLTAVKNQGSCGSCWSFSTTGALEG 176
           + +RLRLPVDTNAAPILPTENLPI FDWRER  +T VKNQGSCGSCWSFSTTGA+EG
Sbjct: 121 RGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVTRVKNQGSCGSCWSFSTTGAIEG 177

[Close]

BLAST of CmaCh19G006810 vs. NCBI nr
Match: KAG7011723.1 (Cysteine protease RD19A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 283.1 bits (723), Expect = 1.6e-72
Identity = 148/191 (77.49%), Postives = 153/191 (80.10%), Query Frame = 0


Query: 1   MDRSFFLF----AATAATLCSSESLASSHSIECDGDTLIRQVIDDGDFKRHSLGAEHHFS 60
           MDRSFFL     A  AATLCSSE LAS HS+ECDGDTLIRQV++DGDFKR  LGAEHHFS
Sbjct: 1   MDRSFFLLAVIAATAAATLCSSEPLASPHSVECDGDTLIRQVVNDGDFKRLPLGAEHHFS 60

Query: 61  LFKRRFGKSYATEEEHDLRLKIFKANMRQALRHQSFDPSVIHGVTQFSDLIPSEFREAFL 120
           LFKRRFGKSYATEEEHDLR KIFKANMRQAL  QSFDPS IHGVTQFSDL PSEFREAFL
Sbjct: 61  LFKRRFGKSYATEEEHDLRFKIFKANMRQALCQQSFDPSTIHGVTQFSDLTPSEFREAFL 120

Query: 121 GLQRNRLRLPVDTNAAPILPTENLPIHFDWRERSVLTAVKNQG------------SCGSC 176
           GL+RNRLRLPVDTN A ILPTENLPIHFDWRER  +TAVKNQ                  
Sbjct: 121 GLRRNRLRLPVDTNTATILPTENLPIHFDWRERGAVTAVKNQDIRLFHLVTFDFWILRIL 180

[Close]

BLAST of CmaCh19G006810 vs. NCBI nr
Match: XP_038887474.1 (probable cysteine protease RD19B [Benincasa hispida])

HSP 1 Score: 278.1 bits (710), Expect = 5.2e-71
Identity = 142/179 (79.33%), Postives = 152/179 (84.92%), Query Frame = 0


Query: 1   MDRSFFLF---AATAATLCSSESLASSHSIECDGDTLIRQVIDDGDF-KRHSLGAEHHFS 60
           MD SF LF   AA  ATLCSSESL S  SIE DGD LIRQV+DDG+F  R  LGAEHHFS
Sbjct: 1   MDWSFSLFAVIAAATATLCSSESLTSPRSIERDGDPLIRQVVDDGNFNNRLPLGAEHHFS 60

Query: 61  LFKRRFGKSYATEEEHDLRLKIFKANMRQALRHQSFDPSVIHGVTQFSDLIPSEFREAFL 120
           LFK+RFGKSYATEEEHD R KIF+ANMR+A RHQSFDPS IHG+TQFSDL P EFR+AFL
Sbjct: 61  LFKQRFGKSYATEEEHDRRFKIFEANMRRAQRHQSFDPSAIHGITQFSDLTPFEFRKAFL 120

Query: 121 GLQRNRLRLPVDTNAAPILPTENLPIHFDWRERSVLTAVKNQGSCGSCWSFSTTGALEG 176
           GL+ +RLRLPVDTNAAPILPTENLPI FDWRER  +T VKNQGSCGSCWSFSTTGALEG
Sbjct: 121 GLRGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVTPVKNQGSCGSCWSFSTTGALEG 179

[Close]

BLAST of CmaCh19G006810 vs. TAIR 10
Match: AT4G16190.1 (Papain family cysteine protease )

HSP 1 Score: 188.7 bits (478), Expect = 3.9e-48
Identity = 97/176 (55.11%), Postives = 116/176 (65.91%), Query Frame = 0


Query: 1   MDRSFFLFAATAATLCSS-ESLASSHSIECDGDTLIRQVIDDGDFKRHSLGAEHHFSLFK 60
           MDR  F F   A  L  S  S   S  +       IRQV+ + +     L AEHHF+LFK
Sbjct: 1   MDRVVFFFLIAATLLAGSLGSTVISGEVTDGFVNPIRQVVPE-ENDEQLLNAEHHFTLFK 60

Query: 61  RRFGKSYATEEEHDLRLKIFKANMRQALRHQSFDPSVIHGVTQFSDLIPSEFREAFLGLQ 120
            ++ K+YAT+ EHD R ++FKAN+R+A R+Q  DPS +HGVTQFSDL P EFR  FLGL+
Sbjct: 61  SKYEKTYATQVEHDHRFRVFKANLRRARRNQLLDPSAVHGVTQFSDLTPKEFRRKFLGLK 120

Query: 121 RNRLRLPVDTNAAPILPTENLPIHFDWRERSVLTAVKNQGSCGSCWSFSTTGALEG 176
           R   RLP DT  APILPT +LP  FDWRE+  +T VKNQG CGSCWSFS  GALEG
Sbjct: 121 RRGFRLPTDTQTAPILPTSDLPTEFDWREQGAVTPVKNQGMCGSCWSFSAIGALEG 175

[Close]

BLAST of CmaCh19G006810 vs. TAIR 10
Match: AT4G39090.1 (Papain family cysteine protease )

HSP 1 Score: 188.0 bits (476), Expect = 6.6e-48
Identity = 94/153 (61.44%), Postives = 110/153 (71.90%), Query Frame = 0


Query: 23  SSHSIECDGDTLIRQVIDDGDFKRHSLGAEHHFSLFKRRFGKSYATEEEHDLRLKIFKAN 82
           SS  +    D +IRQV+  G  +   L +E HFSLFKR+FGK YA+ EEHD R  +FKAN
Sbjct: 21  SSSDVNDGDDLVIRQVV--GGAEPQVLTSEDHFSLFKRKFGKVYASNEEHDYRFSVFKAN 80

Query: 83  MRQALRHQSFDPSVIHGVTQFSDLIPSEFREAFLGLQRNRLRLPVDTNAAPILPTENLPI 142
           +R+A RHQ  DPS  HGVTQFSDL  SEFR+  LG+ R+  +LP D N APILPTENLP 
Sbjct: 81  LRRARRHQKLDPSATHGVTQFSDLTRSEFRKKHLGV-RSGFKLPKDANKAPILPTENLPE 140

Query: 143 HFDWRERSVLTAVKNQGSCGSCWSFSTTGALEG 176
            FDWR+   +T VKNQGSCGSCWSFS TGALEG
Sbjct: 141 DFDWRDHGAVTPVKNQGSCGSCWSFSATGALEG 170

[Close]

BLAST of CmaCh19G006810 vs. TAIR 10
Match: AT2G21430.1 (Papain family cysteine protease )

HSP 1 Score: 184.1 bits (466), Expect = 9.6e-47
Identity = 92/152 (60.53%), Postives = 111/152 (73.03%), Query Frame = 0


Query: 24  SHSIECDGDTLIRQVIDDGDFKRHSLGAEHHFSLFKRRFGKSYATEEEHDLRLKIFKANM 83
           S S+  D D LIRQV+D+ + K   L +E HF+LFK++FGK Y + EEH  R  +FKAN+
Sbjct: 19  SVSVCGDEDVLIRQVVDETEPK--VLSSEDHFTLFKKKFGKVYGSIEEHYYRFSVFKANL 78

Query: 84  RQALRHQSFDPSVIHGVTQFSDLIPSEFREAFLGLQRNRLRLPVDTNAAPILPTENLPIH 143
            +A+RHQ  DPS  HGVTQFSDL  SEFR   LG+ +   +LP D N APILPT+NLP  
Sbjct: 79  LRAMRHQKMDPSARHGVTQFSDLTRSEFRRKHLGV-KGGFKLPKDANQAPILPTQNLPEE 138

Query: 144 FDWRERSVLTAVKNQGSCGSCWSFSTTGALEG 176
           FDWR+R  +T VKNQGSCGSCWSFSTTGALEG
Sbjct: 139 FDWRDRGAVTPVKNQGSCGSCWSFSTTGALEG 167

[Close]

BLAST of CmaCh19G006810 vs. TAIR 10
Match: AT3G54940.2 (Papain family cysteine protease )

HSP 1 Score: 128.6 bits (322), Expect = 4.8e-30
Identity = 71/155 (45.81%), Postives = 90/155 (58.06%), Query Frame = 0


Query: 25  HSIECDGDTLIRQVIDDGDFKRHSLGAEH---HFSLFKRRFGKSYATEEEHDLRLKIFKA 84
           H +    D  IRQV  D    R +L   H    F LF   +GK+Y+T EE+  RL IF  
Sbjct: 18  HVVASVEDLTIRQVTADNRRIRPNLLGTHTESKFRLFMSDYGKNYSTREEYIHRLGIFAK 77

Query: 85  NMRQALRHQSFDPSVIHGVTQFSDLIPSEFREAFLGL-QRNRLRLPVDTNAAPILPTENL 144
           N+ +A  HQ  DPS +HGVTQFSDL   EF+  + G+      R       AP++  + L
Sbjct: 78  NVLKAAEHQMMDPSAVHGVTQFSDLTEEEFKRMYTGVADVGGSRGGTVGAEAPMVEVDGL 137

Query: 145 PIHFDWRERSVLTAVKNQGSCGSCWSFSTTGALEG 176
           P  FDWRE+  +T VKNQG+CGSCW+FSTTGA EG
Sbjct: 138 PEDFDWREKGGVTEVKNQGACGSCWAFSTTGAAEG 172

[Close]

BLAST of CmaCh19G006810 vs. TAIR 10
Match: AT1G20850.1 (xylem cysteine peptidase 2 )

HSP 1 Score: 89.4 bits (220), Expect = 3.2e-18
Identity = 61/172 (35.47%), Postives = 87/172 (50.58%), Query Frame = 0


Query: 6   FLFAATAATLCSSESLASSHSIECDGDTLIRQVIDDGDFKRHSLGAEHHFSLFKRRFGKS 65
           F  A +AA+L  S S ASSH     G           D + H    E  F  +   F K+
Sbjct: 12  FALALSAASL--SLSFASSHDYSIVG-------YSPEDLESHDKLIE-LFENWISNFEKA 71

Query: 66  YATEEEHDLRLKIFKANMRQALRHQSFDPSVIHGVTQFSDLIPSEFREAFLGLQRNRLRL 125
           Y T EE  LR ++FK N++          S   G+ +F+DL   EF++ +LGL+ + +R 
Sbjct: 72  YETVEEKFLRFEVFKDNLKHIDETNKKGKSYWLGLNEFADLSHEEFKKMYLGLKTDIVRR 131

Query: 126 PVDTNAAPIL--PTENLPIHFDWRERSVLTAVKNQGSCGSCWSFSTTGALEG 176
             + + A       E +P   DWR++  +  VKNQGSCGSCW+FST  A+EG
Sbjct: 132 DEERSYAEFAYRDVEAVPKSVDWRKKGAVAEVKNQGSCGSCWAFSTVAAVEG 173

The following BLAST results are available for this feature:

BLAST of CmaCh19G006810 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SUL1	5.5e-47	55.11	Probable cysteine protease RD19C OS=Arabidopsis thaliana OX=3702 GN=RD19C PE=2 S...	[more]
P43296	9.3e-47	61.44	Cysteine protease RD19A OS=Arabidopsis thaliana OX=3702 GN=RD19A PE=1 SV=1	[more]
P25804	4.6e-46	54.75	Cysteine proteinase 15A OS=Pisum sativum OX=3888 PE=2 SV=1	[more]
P43295	1.3e-45	60.53	Probable cysteine protease RD19B OS=Arabidopsis thaliana OX=3702 GN=RD19B PE=2 S...	[more]
Q10716	1.0e-40	51.81	Cysteine proteinase 1 OS=Zea mays OX=4577 GN=CCP1 PE=2 SV=1	[more]

BLAST of CmaCh19G006810 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K2B5	7.1e-74	79.89	Papain-like cysteine proteinase isoform I OS=Cucumis sativus OX=3659 GN=Csa_7G00...	[more]
A0A1S3BIU9	7.1e-74	80.23	cysteine proteinase RD19a-like OS=Cucumis melo OX=3656 GN=LOC103490359 PE=3 SV=1	[more]
A0A5A7SS65	3.5e-73	79.66	Cysteine proteinase RD19a-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s...	[more]
A0A6J1F4R7	5.1e-64	72.47	probable cysteine protease RD19B OS=Cucurbita moschata OX=3662 GN=LOC111440857 P...	[more]
A0A5D3DFH2	2.1e-62	78.13	Cysteine protease RD19A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]

BLAST of CmaCh19G006810 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004148554.2	1.5e-73	79.89	cysteine protease RD19A [Cucumis sativus] >KGN43159.1 hypothetical protein Csa_0...	[more]
XP_008448073.1	1.5e-73	80.23	PREDICTED: cysteine proteinase RD19a-like [Cucumis melo]	[more]
KAA0033790.1	7.3e-73	79.66	cysteine proteinase RD19a-like [Cucumis melo var. makuwa]	[more]
KAG7011723.1	1.6e-72	77.49	Cysteine protease RD19A, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_038887474.1	5.2e-71	79.33	probable cysteine protease RD19B [Benincasa hispida]	[more]

BLAST of CmaCh19G006810 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16190.1	3.9e-48	55.11	Papain family cysteine protease	[more]
AT4G39090.1	6.6e-48	61.44	Papain family cysteine protease	[more]
AT2G21430.1	9.6e-47	60.53	Papain family cysteine protease	[more]
AT3G54940.2	4.8e-30	45.81	Papain family cysteine protease	[more]
AT1G20850.1	3.2e-18	35.47	xylem cysteine peptidase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013201	Cathepsin propeptide inhibitor domain (I29)	SMART	SM00848	Inhibitor_I29_2	coord: 55..111 e-value: 1.3E-15 score: 67.9
IPR013201	Cathepsin propeptide inhibitor domain (I29)	PFAM	PF08246	Inhibitor_I29	coord: 55..111 e-value: 5.0E-9 score: 36.5
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 140..175 e-value: 7.8E-12 score: 45.7
None	No IPR available	GENE3D	1.10.287.2250		coord: 40..120 e-value: 1.7E-17 score: 65.1
None	No IPR available	GENE3D	3.90.70.10	Cysteine proteinases	coord: 134..175 e-value: 1.7E-13 score: 53.2
None	No IPR available	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 29..175
None	No IPR available	PANTHER	PTHR12411:SF783	CYSTEINE PROTEASE RD19C-RELATED	coord: 29..175
IPR000169	Cysteine peptidase, cysteine active site	PROSITE	PS00139	THIOL_PROTEASE_CYS	coord: 158..169
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 53..175

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh19G006810.1	CmaCh19G006810.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005615	extracellular space
cellular_component	GO:0005764	lysosome
molecular_function	GO:0004197	cysteine-type endopeptidase activity
molecular_function	GO:0008234	cysteine-type peptidase activity